Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,296,535	A→G	T43A (ACC→GCC)	yraP →	divisome‑associated lipoprotein YraP

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,296,535	0	A	G	100.0%	179.2 / NA	50	T43A (ACC→GCC)	yraP	divisome‑associated lipoprotein YraP
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (25/25);  total (25/25)

AAGGAGAATACATGAAGGCATTATCGCCAATCGCAGTCCTTATTTCCGCGCTGCTGTTGCAAGGTTGTGTTGCCGCTGCCGTAGTGGGTACCGCTGCTGTGGGTACCAAAGCCGCAACTGACCCACGCAGTGTCGGCACCCAGGTGGACGATGGTACCCTGGAAGTGCGCGTGAACAGCGCATTGTCGAAAGACGAACAGATTAAGAAAGAAGCGCGCATTAATGTAACGGCCTATCAGGGCAAAGTGCTGCTGGTTGGGCAGTCACCAA  >  NC_000913/3296398‑3296667                                                                                                                                          |                                                                                                                                     aaGGAGAATACATGAAGGCATTATCGCCAATCGCAGTCCTTATTTCCGCGCTGCTGTTGCAAGGTTGTGTTGCCGCTGCCGTAGTGGGTACCGCTGCTGTGGGTACCAAAGCCGCAACTGACCCACGCAGTGTCGGCGcc                                                                                                                                    >  1:800419/1‑140 (MQ=255)    gagaATACATGAAGGCATTATCGCCAATCGCAGTCCTTATTTCCGCGCTGCTGTTGCAAGGTTGTGTTGCCGCTGCCGTAGTGGGTACCGCTGCTGTGGGTACCAAAGCCGCAACTGACCCACGCAGTGTCGGCGCCCAg                                                                                                                                 >  2:482326/1‑140 (MQ=255)    gagaATACATGAAGGCATTATCGCCAATCGCAGTCCTTATTTCCGCGCTGCTGTTGCAAGGTTGTGTTGCCGCTGCCGTAGTGGGTACCGCTGCTGTGGGTACCAAAGCCGCAACTGACCCACGCAGTGTCGGCGCCCAg                                                                                                                                 >  2:452311/1‑140 (MQ=255)        aTACATGAAGGCATTATCGCCAATCGCAGTCCTTATTTCCGCGCTGCTGTTGCAAGGTTGTGTTGCCGCTGCCGTAGTGGGTACCGCTGCTGTGGGTACCAAAGCCGCAACTGACCCACGCAGTGTCGGCGCCCAGGTgg                                                                                                                             >  2:132195/1‑140 (MQ=255)        aTACATGAAGGCATTATCGCCAATCGCAGTCCTTATTTCCGCGCTGCTGTTGCAAGGTTGTGTTGCCGCTGCCGTAGTGGGTACCGCTGCTGTGGGTACCAAAGCCGCAACTGACCCACGCAGTGTCGGCGCCCAGGTgg                                                                                                                             >  2:933879/1‑140 (MQ=255)         tACATGAAGGCATTATCGCCAATCGCAGTCCTTATTTCCGCGCTGCTGTTGCAAGGTTGTGTTGCCGCTGCCGTAGTGGGTACCGCTGCTGTGGGTACCAAAGCCGCAACTGACCCACGCAGTGTCGGCGCCCAGGTggg                                                                                                                            >  2:278014/1‑139 (MQ=255)               aaGGCATTATCGCCAATCGCAGTCCTTATTTCCGCGCTGCTGTTGCAAGGTTGTGTTGCCGCTGCCGTAGTGGGTACCGCTGCTGTGGGTACCAAAGCCGCAACTGACCCACGCAGTGTCGGCGCCCAGGTGGACGATgg                                                                                                                      <  1:132195/140‑1 (MQ=255)               aaGGCATTATCGCCAATCGCAGTCCTTATTTCCGCGCTGCTGTTGCAAGGTTGTGTTGCCGCTGCCGTAGTGGGTACCGCTGCTGTGGGTACCAAAGCCGCAACTGACCCACGCAGTGTCGGCGCCCAGGTGGACGATgg                                                                                                                      <  1:278014/140‑1 (MQ=255)               aaGGCATTATCGCCAATCGCAGTCCTTATTTCCGCGCTGCTGTTGCAAGGTTGTGTTGCCGCTGCCGTAGTGGGTACCGCTGCTGTGGGTACCAAAGCCGCAACTGACCCACGCAGTGTCGGCGCCCAGGTGGACGATgg                                                                                                                      <  1:405059/140‑1 (MQ=255)               aaGGCATTATCGCCAATCGCAGTCCTTATTTCCGCGCTGCTGTTGCAAGGTTGTGTTGCCGCTGCCGTAGTGGGTACCGCTGCTGTGGGTACCAAAGCCGCAACTGACCCACGCAGTGTCGGCGCCCAGGTGGACGATgg                                                                                                                      <  1:939133/140‑1 (MQ=255)                 ggCATTATCGCCAATCGCAGTCCTTATTTCCGCGCTGCTGTTGCAAGGTTGTGTTGCCGCTGCCGTAGTGGGTACCGCTGCTGTGGGTACCAAAGCCGCAACTGACCCACGCAGTGTCGGCGCCCAGGTGGACGATGGTa                                                                                                                    >  2:368801/1‑140 (MQ=255)                   cATTATCGCCAATCGCAGTCCTTATTTCCGCGCTGCTGTTGCAAGGTTGTGTTGCCGCTGCCGTAGTGGGTACCGCTGCTGTGGGTACCAAAGCCGCAACTGACCCACGCAGTGTCGGCGCCCAGGTGGACGATGGTAcc                                                                                                                  >  2:431434/1‑140 (MQ=255)                      tATCGCCAATCGCAGTCCTTATTTCCGCGCTGCTGTTGCAAGGTTGTGTTGCCGCTGCCGTAGTGGGTACCGCTGCTGTGGGTACCAAAGCCGCAACTGACCCACGCAGTGTCGGCGCCCAGGTGGACGATGGTACCCTg                                                                                                               >  2:239415/1‑140 (MQ=255)                            cAATCGCAGTCCTTATTTCCGCGCTGCTGTTGCAAGGTTGTGTTGCCGCTGCCGTAGTGGGTACCGCTGCTGTGGGTACCAAAGCCGCAACTGACCCACGCAGTGTCGGCGCCCAGGTGGACGATGGTACCCTGGAAGTg                                                                                                         >  1:614889/1‑140 (MQ=255)                                    gTCCTTATTTCCGCGCTGCTGTTGCAAGGTTGTGTTGCCGCTGCCGTAGTGGGTACCGCTGCTGTGGGTACCAAAGCCGCAACTGACCCACGCAGTGTCGGCGCCCAGGTGGACGATGGTACCCTGGAAGTGCGCGTGaa                                                                                                 >  2:848548/1‑140 (MQ=255)                                           tttCCGCGCTGCTGTTGCAAGGTTGTGTTGCCGCTGCCGTAGTGGGTACCGCTGCTGTGGGTACCAAAGCCGCAACTGACCCACGCAGTGTCGGCGCCCAGGTGGACGATGGTACCCTGGAAGTGCGCGTGAACAGCGCa                                                                                          <  1:933879/140‑1 (MQ=255)                                                          tGCAAGGTTGTGTTGCCGCTGCCGTAGTGGGTACCGCTGCTGTGGGTACCAAAGCCGCAACTGACCCACGCAGTGTCGGCGCCCAGGTGGACGATGGTACCCTGGAAGTGCGCGTGAACAGCGCATTGTCGAAAGACGaa                                                                           <  2:767523/140‑1 (MQ=255)                                                          tGCAAGGTTGTGTTGCCGCTGCCGTAGTGGGTACCGCTGCTGTGGGTACCAAAGCCGCAACTGACCCACGCAGTGTCGGCGCCCAGGTGGACGATGGTACCCTGGAAGTGCGCGTGAACAGCGCATTGTCGAAAGACGaa                                                                           <  1:720579/140‑1 (MQ=255)                                                          tGCAAGGTTGTGTTGCCGCTGCCGTAGTGGGTACCGCTGCTGTGGGTACCAAAGCCGCAACTGACCCACGCAGTGTCGGCGCCCAGGTGGACGATGGTACCCTGGAAGTGCGCGTGAACAGCGCATTGTCGAAAGACGaa                                                                           <  1:239415/140‑1 (MQ=255)                                                          tGCAAGGTTGTGTTGCCGCTGCCGTAGTGGGTACCGCTGCTGTGGGTACCAAAGCCGCAACTGACCCACGCAGTGTCGGCGCCCAGGTGGACGATGGTACCCTGGAAGTGCGCGTGAACAGCGCATTGTCGAAAGACGaa                                                                           <  1:452311/140‑1 (MQ=255)                                                          tGCAAGGTTGTGTTGCCGCTGCCGTAGTGGGTACCGCTGCTGTGGGTACCAAAGCCGCAACTGACCCACGCAGTGTCGGCGCCCAGGTGGACGATGGTACCCTGGAAGTGCGCGTGAACAGCGCATTGTCGAAAGACGaa                                                                           <  1:32751/140‑1 (MQ=255)                                                          tGCAAGGTTGTGTTGCCGCTGCCGTAGTGGGTACCGCTGCTGTGGGTACCAAAGCCGCAACTGACCCACGCAGTGTCGGCGCCCAGGTGGACGATGGTACCCTGGAAGTGCGCGTGAACAGCGCATTGTCGAAAGACGaa                                                                           <  1:431434/140‑1 (MQ=255)                                                             aaGGTTGTGTTGCCGCTGCCGTAGTGGGTACCGCTGCTGTGGGTACCAAAGCCGCAACTGACCCACGCAGTGTCGGCGCCCAGGTGGACGATGGTACCCTGGAAGTGCGCGTGAACAGCGCATTGTCGAAAGACGAACAg                                                                        <  1:848548/140‑1 (MQ=255)                                                               ggTTGTGTTGCCGCTGCCGTAGTGGGTACCGCTGCTGTGGGTACCAAAGCCGCAACTGACCCACGCAGTGTCGGCGCCCAGGTGGACGATGGTACCCTGGAAGTGCGCGTGAACAGCGCATTGTCGAAAGACGAACAGAt                                                                      <  2:256313/140‑1 (MQ=255)                                                                gTTGTGTTGCCGCTGCCGTAGTGGGTACCGCTGCTGTGGGTACCAAAGCCGCAACTGACCCACGCAGTGTCGGCGCCCAGGTGGACGATGGTACCCTGGAAGTGCGCGTGAACAGCGCATTGTCGAAAGACGAACAGAtt                                                                     >  2:876052/1‑140 (MQ=255)                                                                           gCTGCCGTAGTGGGTACCGCTGCTGTGGGTACCAAAGCCGCAACTGACCCACGCAGTGTCGGCGCCCAGGTGGACGATGGTACCCTGGAAGTGCGCGTGAACAGCGCATTGTCGAAAGACGAACAGAt                                                                      >  2:943055/1‑128 (MQ=255)                                                                           gCTGCCGTAGTGGGTACCGCTGCTGTGGGTACCAAAGCCGCAACTGACCCACGCAGTGTCGGCGCCCAGGTGGACGATGGTACCCTGGAAGTGCGCGTGAACAGCGCATTGTCGAAAGACGAACAGATTAAGAAAGAAgc                                                          >  2:418604/1‑140 (MQ=255)                                                                           gCTGCCGTAGTGGGTACCGCTGCTGTGGGTACCAAAGCCGCAACTGACCCACGCAGTGTCGGAGCCCAGGTGGACGATGGTACCCTGGAAGTGCGCGTGAACAGCGCATTGTCGAAAGACGAACAGAt                                                                      <  1:943055/128‑1 (MQ=255)                                                                            cTGCCGTAGTGGGTACCGCTGCTGTGGGTACCAAAGCCGCAACTGACCCACGCAGTGTCGGCGCCCAGGTGGACGATGGTACCCTGGAAGTGCGCGTGAACAGCGCATTGTCGAAAGACGAACAGATTAAGAAAGAAgcg                                                         <  1:674913/140‑1 (MQ=255)                                                                            cTGCCGTAGTGGGTACCGCTGCTGTGGGTACCAAAGCCGCAACTGACCCACGCAGTGTCGGCGCCCAGGTGGACGATGGTACCCTGGAAGTGCGCGTGAACAGCGCATTGTCGAAAGACGAACAGATTAAGAAAGAAgcg                                                         <  1:171546/140‑1 (MQ=255)                                                                                 gTAGTGGGTACCGCTGCTGTGGGTACCAAAGCCGCAACTGACCCACGCAGTGTCGGCGCCCAGGTGGACGATGGTACCCTGGAAGTGCGCGTGAACAGCGCATTGTCGAAAGACGAACAGATTAAGAAAGAAGCGCGCAt                                                    <  1:482326/140‑1 (MQ=255)                                                                                       ggTACCGCTGCTGTGGGTACCAAAGCCGCAACTGACCCACGCAGTGTCGGCGCCCAGGTGGACGATGGTACCCTGGAAGTGCGCGTGAACAGCGCATTGTCGAAAGACGAACAGATTAAGAAAGAAGCGCGCATTAATGt                                              <  1:186671/140‑1 (MQ=255)                                                                                       ggTACCGCTGCTGTGGGTACCAAAGCCGCAACTGACCCACGCAGTGTCGGCGCCCAGGTGGACGATGGTACCCTGGAAGTGCGCGTGAACAGCGCATTGTCGAAAGACGAACAGATTAAGAAAGAAGCGCGCATTAATGt                                              <  2:614889/140‑1 (MQ=255)                                                                                         tACCGCTGCTGTGGGTACCAAAGCCGCAACTGACCCACGCAGTGTCGGCGCCCAGGTGGACGATGGTACCCTGGAAGTGCGCGTGAACAGCGCATTGTCGAAAGACGAACAGATTAAGAAAGAAGCGCGCATTAATGTaa                                            >  2:275109/1‑140 (MQ=255)                                                                                              ctgctgTGGGTACCAAAGCCGCAACTGACCCACGCAGTGTCGGCGCCCAGGTGGACGATGGTACCCTGGAAGTGCGCGTGAACAGCGCATTGTCGAAAGACGAACAGATTAAGAAAGAAGCGCGCATTAATGTAACGGcc                                       >  1:63574/1‑140 (MQ=255)                                                                                              ctgctgTGGGTACC‑AAGCCGCAACTGACCCACGCAGTGTCGGCGCCCAGGTGGACGATGGTACCCTGGAAGTGCGCGTGAACAGCGCATTGTCGAAAGACGAACAGATTAAGAAAGAAGCGCGCATTAATGt                                              >  2:895221/1‑132 (MQ=255)                                                                                              ctgctgTGGGTACC‑AAGCCGCAACTGACCCACGCAGTGTCGGCGCCCAGGTGGACGATGGTACCCTGGAAGTGCGCGTGAACAGCGCATTGTCGAAAGACGAACAGATTAAGAAAGAAGCGCGCATTAATGt                                              <  1:895221/132‑1 (MQ=255)                                                                                                 ctgTGGGTACCAAAGCCGCAACTGACCCACGCAGTGTCGGCGCCCAGGTGGACGATGGTACCCTGGAAGTGCGCGTGAACAGCGCATTGTCGAAAGACGAACAGATTAAGAAAGAAGCGCGCATTAATGTAACGGCCTAt                                    >  1:496665/1‑140 (MQ=255)                                                                                                   gtgGGTACCAAAGCCGCAACTGACCCACGCAGTGTCGGCGCCCAGGTGGACGATGGTACCCTGGAAGTGCGCGTGAACAGCGCATTGTCGAAAGACGAACAGATTAAGAAAGAAGCGCGCATTAATGTAACGGCCTATCa                                  >  1:402673/1‑140 (MQ=255)                                                                                                         aCCAAAGCCGCAACTGACCCACGCAGTGTCGGCGCCCAGGTGGACGATGGTACCCTGGAAGTGCGCGTGAACAGCGCATTGTCGAAAGACGAACAGATTAAGAAAGAAGCGCGCATTAATGTAACGGcc                                       <  1:433026/129‑1 (MQ=255)                                                                                                         aCCAAAGCCGCAACTGACCCACGCAGTGTCGGCGCCCAGGTGGACGATGGTACCCTGGAAGTGCGCGTGAACAGCGCATTGTCGAAAGACGAACAGATTAAGAAAGAAGCGCGCATTAATGTAACGGCCTATCAGGGCaa                            >  2:15747/1‑140 (MQ=255)                                                                                                         aCCAAAGCCACAACTGACCCACGCAGTGTCGGCGCCCAGGTGGACGATGGTACCCTGGAAGTGCGCGTGAACAGCGCATTGTCGAAAGACGAACAGATTAAGAAAGAAGCGCGCATTAATGTAACGGcc                                       >  2:433026/1‑129 (MQ=255)                                                                                                           cAAAGCCGCAACTGACCCACGCAGTGTCGGCGCCCAGGTGGACGATGGTACCCTGGAAGTGCGCGTGAACAGCGCATTGTCGAAAGACGAACAGATTAAGAAAGAAGCGCGCATTAATGTAACGGCCTATCAGGGCAAAg                          <  2:402673/140‑1 (MQ=255)                                                                                                               gCCGCAACTGACCCACGCAGTGTCGGCGCCCAGGTGGACGATGGTACCCTGGAAGTGCGCGTGAACAGCGCATTGTCGAAAGACGAACAGATTAAGAAAGAAGCGCGCATTAATGTAACGGCCTATCAGGGCAAAGtgct                      <  2:496665/140‑1 (MQ=255)                                                                                                                ccGCAACTGACCCACGCAGTGTCGGCGCCCAGGTGGACGATGGTACCCTGGAAGTGCGCGTGAACAGCGCATTGTCGAAAGACGAACAGATTAAGAAAGAAGCGCGCATTAATGTAACGGCCTATCAGGGCAAAGtgctg                     >  2:943795/1‑140 (MQ=255)                                                                                                                         aCCCACGCAGTGTCGGCGCCCAGGTGGACGATGGTACCCTGGAAGTGCGCGTGAACAGCGCATTGTCGAAAGCCGAACAGATTAAGAAAGAAGCGCGCCTTAATGTAACGGCCTATCAGGGCAAAGTGCTGCTGGTTggg            >  2:708962/1‑140 (MQ=255)                                                                                                                         aCCCACGCAGTGTCGGCGCCCAGGTGGACGATGGTACCCTGGAAGTGCGCGTGAACAGCGCATTGTCGAAAGACGAACAGATTAAGAAAGAAGCGCGCATTAATGTAACGGCCTATCAGGGCAAAGTGCTGCTGGTTggg            <  2:1072297/140‑1 (MQ=255)                                                                                                                                   tgtCGGCGCCCAGGTGGACGATGGTACCCTGGAAGTGCGCGTGAACAGCGCATTGTCGAAAGACGAACAGATTAAGAAAGAAGCGCGCATTAATGTAACGGCCTATCAGGGCAAAGTGCTGCTGGTTGGGc           >  2:218353/1‑131 (MQ=255)                                                                                                                                   tgtCGGCGCCCAGGTGGACGATGGTACCCTGGAAGTGCGCGTGAACAGCGCATTGTCGAAAGACGAACAGATTAAGAAAGAAGCGCGCATTAATGTAACGGCCTATCAGGGCAAAGTGCTGCTGGTTGGGc           <  1:218353/131‑1 (MQ=255)                                                                                                                                   tgtCGGCGCCCAGGTGGACGATGGTACCCTGGAAGTGCGCGTGAACAGCGCATTGTCGAAAGACGAACAGATTAAGAAAGAAGCGCGCATTAATGTAACGGCCTATCAGGGCAAAGTGCTGCTGGTTGGGCAGTCACCaa  >  1:979749/1‑140 (MQ=255)                                                                                                                                          |                                                                                                                                     AAGGAGAATACATGAAGGCATTATCGCCAATCGCAGTCCTTATTTCCGCGCTGCTGTTGCAAGGTTGTGTTGCCGCTGCCGTAGTGGGTACCGCTGCTGTGGGTACCAAAGCCGCAACTGACCCACGCAGTGTCGGCACCCAGGTGGACGATGGTACCCTGGAAGTGCGCGTGAACAGCGCATTGTCGAAAGACGAACAGATTAAGAAAGAAGCGCGCATTAATGTAACGGCCTATCAGGGCAAAGTGCTGCTGGTTGGGCAGTCACCAA  >  NC_000913/3296398‑3296667

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑