Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,387,282	T→C	T303A (ACG→GCG)	aaeB ←	aromatic carboxylic acid efflux pump subunit AaeB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,387,282	0	T	C	100.0%	211.9 / NA	58	T303A (ACG→GCG)	aaeB	aromatic carboxylic acid efflux pump subunit AaeB
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (30/28);  total (30/28)

TTTTACTTCCGGTTCGCCTTGCAGGATCTCTTCTTCGGTGGCGTTGATTTTTGTGTTACTGATAACGCCGCGCTTGAGAAGCTGATAACGCGTTGCCGCCGCGACCCAGCTATAAATGGTGACAGGCGTTTCCCGTTCCCCGGTCCAGGCGATAACTCTCCGCAGGCGTTTGAGCTGCTTGTGGACGTCCTGCGCGGTTTCTACCGGCGTGTCAAAAAATTCGCGGAAAGTATCAGTGATCAATTCCGGGCGCGTATTCTGA  >  NC_000913/3387154‑3387415                                                                                                                                 |                                                                                                                                      ttttACTTCCGGTTCGCCTTGCAGGATCTCTTCTTCGGTGGCGTTGATTTTTGTGTTACTGATAACGCCGCGCTTGAGAAGCTGATAACGCGTTGCCGCCGCGACCCAGCTATAAATGGTGACAGGCGCTTCCCGTTccc                                                                                                                            <  1:276112/140‑1 (MQ=255)    tACTTCCGGTTCGCCTTGCAGGATCTCTTCTTCGGTGGCGTTGATTTTTGTGTTACTGATAACGCCGCGCTTGAGAAGCTGATAACGCGTTGCCGCCGCGACCCAGCTATAAATGGTGACAGGCGCTTCCCGTTCCCCgg                                                                                                                         >  2:378319/1‑140 (MQ=255)     aCTTCCGGTTCGCCTTGCAGGATCTCTTCTTCGGTGGCGTTGATTTTTGTGTTACTGATAACGCCGCGCTTGAGAAGCTGATAACGCGTTGCCGCCGCGACCCAGCTATAAATGGTGACAGGCGCTTCCCGTTc                                                                                                                              <  1:172322/134‑1 (MQ=255)     aCTTCCGGTTCGCCTTGCAGGATCTCTTCTTCGGTGGCGTTGATTTTTGTGTTACTGATAACGCCGCGCTTGAGAAGCTGATAACGCGTTGCCGCCGCGACCCAGCTATAAATGGTGACAGGCGCTTCCCGTTc                                                                                                                              >  2:172322/1‑134 (MQ=255)          cGGTTCGCCTTGCAGGATCTCTTCTTCGGTGGCGTTGATTTTTGTGTTACTGATAACGCCGCGCTTGAGAAGCTGATAACGCGTTGCCGCCGCGACCCAGCTATAAATGGTGACAGGCGCTTCCCGTTCCCCGGTCCAgg                                                                                                                   >  2:996356/1‑140 (MQ=255)          cGGTTCGCCTTGCAGGATCTCTTCTTCGGTGGCGTTGATTTTTGTGTTACTGATAACGCCGCGCTTGAGAAGCTGATAACGCGTTGCCGCCGCGACCCAGCTATAAATGGTGACAGGCGCTTCCCGTTCCCCGGTCCAgg                                                                                                                   >  2:321245/1‑140 (MQ=255)                 ccTTGCAGGATCTCTTCTTCGGTGGCGTTGATTTTTGTGTTACTGATAACGCCGCGCTTGAGAAGCTGATAACGCGTTGCCGCCGCGACCCAGCTATAAATGGTGACAGGCGCTTCCCGTTCCCCGGTCCAGGCGATAAc                                                                                                            >  2:629384/1‑140 (MQ=255)                  cTTGCAGGATCTCTTCTTCGGTGGCGTTGATTTTTGTGTTACTGATAACGCCGCGCTTGAGAAGCTGATAACGCGTTGCCGCCGCGACCCAGCTATAAATGGTGACAGGCGCTTCCCGTTCCCCGGTCCAGGCGATAAct                                                                                                           <  1:378319/140‑1 (MQ=255)                              cttcttCGGTGGCGTTGATTTTTGTGTTACTGATAACGCCGCGCTTGAGAAGCTGATAACGCGTTGCCGCCGCGACCCAGCTATAAATGGTGACAGGCGCTTCCCGTTCCCCGGTCCAGGCGATAACTCTCCGCAGGCGt                                                                                               >  1:789659/1‑140 (MQ=255)                              cttcttCGGTGGCGTTGATTTTTGTGTTACTGATAACGCCGCGCTTGAGAAGCTGATAACGCGTTGCCGCCGCGACCCAGCTATAAATGGTGACAGGCGCTTCCCGTTCCCCGGTCCAGGCGATAACTCTCCGCAGGCGt                                                                                               >  2:1074370/1‑140 (MQ=255)                              cttcttCGGTGGCGTTGATTTTTGTGTTACTGATAACGCCGCGCTTGAGAAGCTGATAACGCGTTGCCGCCGCGACCCAGCTATAAATGGTGACAGGCGCTTCCCGTTCCCCGGTCCAGGCGATAACTCTCCGCAGGCGt                                                                                               >  2:630855/1‑140 (MQ=255)                               ttcttcGGTGGCGTTGATTTTTGTGTTACTGATAACGCCGCGCTTGAGAAGCTGATAACGCGTTGCCGCCGCGACCCAGCTATAAATGGTGACAGGCGCTTCCCGTTCCCCGGTCCAGGCGATAACTCTCCGCAGGCGtt                                                                                              <  2:2206/140‑1 (MQ=255)                                   tcGGTGGCGTTGATTTTTGTGTTACTGATAACGCCGCGCTTGAGAAGCTGATAACGCGTTGCCGCCGCGACCCAGCTATAAATGGTGACAGGCGCTTCCCGTTCCCCGGTCCAGGCGATAACTCTCCGCAGGCGTTTGAg                                                                                          <  1:772060/140‑1 (MQ=255)                                   tcGGTGGCGTTGATTTTTGTGTTACTGATAACGCCGCGCTTGAGAAGCTGATAACGCGTTGCCGCCGCGACCCAGCTATAAATGGTGACAGGCGCTTCCCGTTCCCCGGTCCAGGCGATAACTCTCCGCAGGCGTTTGAg                                                                                          <  1:59681/140‑1 (MQ=255)                                   tcGGTGGCGTTGATTTTTGTGTTACTGATAACGCCGCGCTTGAGAAGCTGATAACGCGTTGCCGCCGCGACCCAGCTATAAATGGTGACAGGCGCTTCCCGTTCCCCGGTCCAGGCGATAACTCTCCGCAGGCGTTTGAg                                                                                          <  1:131623/140‑1 (MQ=255)                                    cgctGGCGTTGATTTTTGTGTTACTGATAACGCCGCGCTTGAGAAGCTGATAACGCGTTGCCGCCGCGACCCAGCTATAAATGGTGACAGGCGCTTCCCGTTCCCCGGTCCAGGCGATAACTCTCCGCAGGCGTTTGAgc                                                                                         >  1:264445/4‑140 (MQ=255)                                    cGGTGGCGTTGATTTTTGTGTTACTGATAACGCCGCGCTTGAGAAGCTGATAACGCGTTGCCGCCGCGACCCAGCTATAAATGGTGACAGGCGCTTCCCGTTCCCCGGTCCAGGCGATAACTCTCCGCAGGCGTTTGAgc                                                                                         >  2:518493/1‑140 (MQ=255)                                    cGGTGGCGTTGATTTTTGTGTTACTGATAACGCCGCGCTTGAGAAGCTGATAACGCGTTGCCGCCGCGACCCAGCTATAAATGGTGACAGGCGCTTCCCGTTCCCCGGTCCAGGCGATAACTCTCCGCAGGCGTTTGAg                                                                                          <  2:428693/139‑1 (MQ=255)                                    cGGTGGCGTTGATTTTTGTGTTACTGATAACGCCGCGCTTGAGAAGCTGATAACGCGTTGCCGCCGCGACCCAGCTATAAATGGTGACAGGCGCTTCCCGTTCCCCGGTCCAGGCGATAACTCTCCGCAGGCGTTTGAg                                                                                          >  1:428693/1‑139 (MQ=255)                                      gTGGCGTTGATTTTTGTGTTACTGATAACGCCGCGCTTGAGAAGCTGATAACGCGTTGCCGCCGCGACCCAGCTATAAATGGTGACAGGCGCTTCCCGTTCCCCGGTCCAGGCGATAACTCTCCGCAGGCGTTTGAgctg                                                                                       >  2:148017/1‑140 (MQ=255)                                      gTGGCGTTGATTTTTGTGTTACTGATAACGCCGCGCTTGAGAAGCTGATAACGCGTTGCCGCCGCGACCCAGCTATAAATGGTGACAGGCGCTTCCCGTTCCCCGGTCCAGGCGATAACTCTCCGCAGGCGTTTGAgctg                                                                                       <  1:1072251/140‑1 (MQ=255)                                      gTGGCGTTGATTTTTGTGTTACTGATAACGCCGCGCTTGAGAAGCTGATAACGCGTTGCCGCCGCGACCCAGCTATAAATGGTGACAGGCGCTTCCCGTTCCCCGGTCCAGGCGATAACTCTCCGCAGGCGTTTGAactg                                                                                       <  1:1074370/140‑1 (MQ=255)                                         gCGTTGATTTTTGTGTTACTGATAACGCCGCGCTTGAGAAGCTGATAACGCGTTGCCGCCGCGACCCAGCTATAAATGGTGACAGGCGCTTCCCGTTCCCCGGTCCAGGCGATAACTCTCCGCAGGCGTTTGAGCTGCtt                                                                                    >  2:755063/1‑140 (MQ=255)                                         gCGTTGATTTTTGTGTTACTGATAACGCCGCGCTTGAGAAGCTGATAACGCGTTGCCGCCGCGACCCAGCTATAAATGGTGACAGGCGCTTCCCGTTCCCCGGTCCAGGCGATAACTCTCCGCAGGCGTTTGAGCTGCtt                                                                                    >  1:952629/1‑140 (MQ=255)                                         gCGTTGATTTTTGTGTTACTGATAACGCCGCGCTTGAGAAGCTGATAACGCGTTGCCGCCGCGACCCAGCTATAAATGGTGACAGGCGCTTCCCGTTCCCCGGTCCAGGCGATAACTCTCCGCAGGCGTTTGAGCTGCtt                                                                                    >  1:1067107/1‑140 (MQ=255)                                           gTTGATTTTTGTGTTACTGATAACGCCGCGCTTGAGAAGCTGATAACGCGTTGCCGCCGCGACCCAGCTATAAATGGTGACAGGCGCTTCCCGTTCCCCGGTCCAGGCGATAACTCTCCGCAGGCGTTTGAgc                                                                                         <  2:375897/133‑1 (MQ=255)                                           gTTGATTTTTGTGTTACTGATAACGCCGCGCTTGAGAAGCTGATAACGCGTTGCCGCCGCGACCCAGCTATAAATGGTGACAGGCGCTTCCCGTTCCCCGGTCCAGGCGATAACTCTCCGCAGGCGTTTGAgc                                                                                         >  1:375897/1‑133 (MQ=255)                                                  tttGTGTTACTGATAACGCCGCGCTTGAGAAGCTGATAACGCGTTGCCGCCGCGACCCAGCTATAAATGGTGACAGGCGCTTCCCGTTCCCCGGTCCAGGCGATAACTCTCCGCAGGCGTTTGAGCTGCTTGTGGACGTc                                                                           <  2:709478/140‑1 (MQ=255)                                                      tgtTACTGATAACGCCGCGCTTGAGAAGCTGATAACGCGTTGCCGCCGCGACCCAGCTATAAATGGTGACAGGCGCTTCCCGTTCCCCGGTCCAGGCGATAACTCTCCGCAGGCGTTTGAGCTGCTTGTGGACGTCCTgc                                                                       >  1:234963/1‑140 (MQ=255)                                                       gtTACTGATAACGCCGCGCTTGAGAAGCTGATAACGCGTTGCCGCCGCGACCCAGCTATAAATGGTGACAGGCGCTTCCCGTTCCCCGGTCCAGGCGATAACTCTCCGCAGGCGTTTGAGCTGCTTGTGGACGTCCTgcg                                                                      <  1:629384/140‑1 (MQ=255)                                                         tACTGATAACGCCGCGCTTGAGAAGCTGATAACGCGTTGCCGCCGCGACCCAGCTATAAATGGTGACAGGCGCTTCCCGTTCCCCGGTCCAGGCGATAACTCTCCGCAGGCGTTTGAGCTGCTTGTGGACGTCCTGcgcg                                                                    <  2:234963/140‑1 (MQ=255)                                                          aCTGATAACGCCGCGCTTGAGAAGCTGATAACGCGTTGCCGCCGCGACCCAGCTATAAATGGTGACAGGCGCTTCCCGTTCCCCGGTCCAGGCGATAACTCTCCGCAGGCGTTTGAGCTGCTTGTGGACGTCCTGCGCgg                                                                   >  2:1015480/1‑140 (MQ=255)                                                           cTGATAACGCCGCGCTTGAGAAGCTGATAACGCGTTGCCGCCGCGACCCAGCTATAAATGGTGACAGGCGCTTCCCGTTCCTCGGTCCAGGCGATAACTCTCCGCAGGCGTTTGAGCTGCTTGTGGACGTCCTGCGCGGt                                                                  <  2:561413/140‑1 (MQ=255)                                                              aTAACGCCGCGCTTGAGAAGCTGATAACGCGTTGCCGCCGCGACCCAGCTATAAATGGTGACAGGCGCTTCCCGTTCCCCGGTCCAGGCGATAACTCTCCGCAGGCGTTTGAGCTGCTTGTGGACGTCCTGCGCGGTTTc                                                               >  2:463520/1‑140 (MQ=255)                                                                     cgcgCTTGAGAAGCTGATAACGCGTTGCCGCCGCGACCCAGCTATAAATGGTGACAGGCGCTTCCCGTTCCCCGGTCCAGGCGATAACTCTCCGCAGGCGTTTGAGCTGCTTGTGGACGTCCTGCGCGGTTTCTACCGGc                                                        >  1:326180/1‑140 (MQ=255)                                                                     cgcgCTTGAGAAGCTGATAACGCGTTGCCGCCGCGACCCAGCTATAAATGGTGACAGGCGCTTCCCGTTCCCCGGTCCAGGCGATAACTCTCCGCAGGCGTTTGAGCTGCTTGTGGACGTCCTGCGCGGTTTCTACCGGc                                                        >  1:322620/1‑140 (MQ=255)                                                                      gcgcTTGAGAAGCTGATAACGCGTTGCCGCCGCGACCCAGCTATAAATGGTGACAGGCGCTTCCCGTTCCCCGGTCCAGGCGATAACTCTCCGCAGGCGTTTGAGCTGCTTGTGGACGTCCTGCGCGGTTTCTACCGGCg                                                       >  1:890371/1‑140 (MQ=255)                                                                                    gATAACGCGTTGCCGCCGCGACCCAGCTATAAATGGTGACAGGCGCTTCCCGTTCCCCGGTCCAGGCGATAACTCTCCGCAGGCGTTTGAGCTGCTTGTGGACGTCCTGCGCGGTTTCTACCGGCGTGTCAAAAAATTcg                                         >  1:129672/1‑140 (MQ=255)                                                                                    gATAACGCGTTGCCGCCGCGACCCAGCTATAAATGGTGACAGGCGCTTCCCGTTCCCCGGTCCAGGCGATAACTCTCCGCAGGCGTTTGAGCTGCTTGTGGACGTCCTGCGCGGTTTCTACCGGCGTGTCAAAAAATTcg                                         >  1:631744/1‑140 (MQ=255)                                                                                      tAACGCGTTGCCGCCGCGACCCAGCTATAAATGGTGACAGGCGCTTCCCGTTCCCCGGTCCAGGCGATAACTCTCCGCAGGCGTTTGAGCTGCTTGTGGACGTCCTGCGCGGTTTCTACCGGCGTGTCAAAAAATTcgcg                                       <  2:789659/140‑1 (MQ=255)                                                                                      tAACGCGTTGCCGCCGCGACCCAGCTATAAATGGTGACAGGCGCTTCCCGTTCCCCGGTCCAGGCGATAACTCTCCGCAGGCGTTTGAGCTGCTTGTGGACGTCCTGCGCGGTTTCTACCGGCGTGTCAAAAAATTcgcg                                       <  2:1067107/140‑1 (MQ=255)                                                                                        aCGCGTTGCCGCCGCGACCCAGCTATAAATGGTGACAGGCGCTTCCCGTTCCCCGGTCCAGGCGATAACTCTCCGCAGGCGTTTGAGCTGCTTGTGGACGTCCTGCGCGGTTTCTACCGGCGTGTCAAAAAATTCGCGGa                                     <  2:169634/140‑1 (MQ=255)                                                                                          gcgTTGCCGCCGCGACCCAGCTATAAATGGTGACAGGCGCTTCCCGTTCCCCGGTCCAGGCGATAACTCTCCGCAGGCGTTTGAGCTGCTTGTGGACGTCCTGCGCGGTTTCTACCGGCGTGTCAAAAAATTCGCGGaaa                                   <  1:148017/140‑1 (MQ=255)                                                                                              tGCCGCCGCGACCCAGCTATAAATGGTGACAGGCGCTTCCCGTTCCCCGGTCCAGGCGATAACTCTCCGCAGGCGTTTGAGCTGCTTGTGGACGTCCTGCGCGGTTTCTACCGGCGTGTCAAAAAATTCGCGGAAAGTAt                               <  1:463520/140‑1 (MQ=255)                                                                                               gccgccGCGACCCAGCTATAAATGGTGACAGGCGCTTCCCGTTCCCCGGTCCAGGCGATAACTCTCCGCAGGCGTTTGAGCTGCTTGTGGACGTCCTGCGCGGTTTCTACCGGCGTGTCAAAAAATTCGCGGAAAGTATc                              >  2:110108/1‑140 (MQ=255)                                                                                               gccgccGCGACCCAGCTATAAATGGTGACAGGCGCTTCCCGTTCCCCGGTCCAGGCGATAACTCTCCGCAGGCGTTTGAGCTGCTTGTGGACGTCCTGCGCGGTTTCTACCGGCGTGTCAAAAAATTCGCGGAAAGTATc                              >  1:322501/1‑140 (MQ=255)                                                                                                ccgccgCGACCCAGCTATAAATGGTGACAGGCGCTTCCCGTTCCCCGGTCCAGGCGATAACTCTCCGCAGGCGTTTGAGCTGCTTGTGGACGTCCTGCGCGGTTTCTACCGGCGTGTCAAAAAATTCGCGGAAAGTATCa                             <  1:996356/140‑1 (MQ=255)                                                                                                 cgccgcGACCCAGCTATAAATGGTGACAGGCGCTTCCCGTTCCCCGGTCCAGGCGATAACTCTCCGCAGGCGTTTGAGCTGCTTGTGGACGTCCTGCGCGGTTTCTACCGGCGTGTCAAAAAATTCGCGGAAAGTATCAg                            <  1:1015480/140‑1 (MQ=255)                                                                                                 cgccgcGACCCAGCTATAAATGGTGACAGGCGCTTCCCGTTCCCCGGTCCAGGCGATAACTCTCCGCAGGCGTTTGAGCTGCTTGTGGACGTCCTGCGCGGTTTCTACCGGCGTGTCAAAAAATTCGCGGAAAGTATCAg                            <  2:264445/140‑1 (MQ=255)                                                                                                             gCTATAAATGGTGACAGGCGCTTCCCGTTCCCCGGTCCAGGCGATAACTCTCCGCAGGCGTTTGAGCTGCTTGTGGACGTCCTGCGCGGTTTCTACCGGCGTGTCAAAAAATTCGCGGAAAGTATCAGTGATCAATTCCg                <  2:631744/140‑1 (MQ=255)                                                                                                                  aaaTGGTGACAGGCGCTTCCCGTTCCCCGGTCCAGGCGATAACTCTCCGCAGGCGTTTGAGCTGCTTGTGGACGTCCTGCGCGGTTTCTACCGGCGTGTCAAAAAATTCGCGGAAAGTATCAGTGATCAATTCCGGgcgc           <  1:321245/140‑1 (MQ=255)                                                                                                                  aaaTGGTGACAGGCGCTTCCCGTTCCCCGGTCCAGGCGATAACTCTCCGCAGGCGTTTGAGCTGCTTGTGGACGTCCTGCGCGGTTTCTACCGGCGTGTCAAAAAATTCGCGGAAAGTATCAGTGATCAATTCCGGgcgc           <  1:57973/140‑1 (MQ=255)                                                                                                                   aaTGGTGACAGGCGCTTCCCGTTCCCCGGTCCAGGCGATAACTCTCCGCAGGCGTTTGAGCTGCTTGTGGACGTCCTGCGCGGTTTCTACCGGCGTGTCAAAAAATTCGCGGAAAGTATCAGTGATCAATTCCGGGcgcg          >  2:446837/1‑140 (MQ=255)                                                                                                                       gTGACAGGCGCTTCCCGTTCCCCGGTCCAGGCGATAACTCTCCGCAGGCGTTTGAGCTGCTTGTGGACGTCCTGCGCGGTTTCTACCGGCGTGTCAAAAAATTCGCGGAAAGTATCAGTGATCAATTCCGGGCGCGTAtt      >  2:591571/1‑140 (MQ=255)                                                                                                                       gTGACAGGCGCTTCCCGTTCCCCGGTCCAGGCGATAACTCTCCGCAGGCGTTTGAGCTGCTTGTGGACGTCCTGCGCGGTTTCTACCGGCGTGTCAAAAAATTCGCGGAAAGTATCAGTGATCAATTCCGGGCGCGTAtt      <  2:326180/140‑1 (MQ=255)                                                                                                                        tGACAGGCGCTTCCCGTTCCCCGGTCCAGGCGATAACTCTCCGCAGGCGTTTGAGCTGCTTGTGGACGTCCTGCGCGGTTTCTACCGGCGTGTCAAAAAATTCGCGGAAAGTATCAGTGATCAATTCCGGGCGCGTATTc     >  1:376691/1‑140 (MQ=255)                                                                                                                        tGACAGGCGCTTCCCGTTCCCCGGTCCAGGCGATAACTCTCCGCAGGCGTTTGAGCTGCTTGTGGACGTCCTGCGCGGTTTCTACCGGCGTGTCAAAAAATTCGCGGAAAGTATCAGTGATCAATTCCGGGCGCGTATTc     >  2:819196/1‑140 (MQ=255)                                                                                                                           cAGGCGCTTCCCGTTCCCCGGTCCAGGCGATAACTCTCCGCAGGCGTTTGAGCTGCTTGTGGACGTCCTGCGCGGTTTCTACCGGCGTGTCAAAAAATTCGCGGAAAGTATCAGTGATCAATTCCGGGCGCGTATTCTGa  <  1:446837/140‑1 (MQ=255)                                                                                                                                 |                                                                                                                                      TTTTACTTCCGGTTCGCCTTGCAGGATCTCTTCTTCGGTGGCGTTGATTTTTGTGTTACTGATAACGCCGCGCTTGAGAAGCTGATAACGCGTTGCCGCCGCGACCCAGCTATAAATGGTGACAGGCGTTTCCCGTTCCCCGGTCCAGGCGATAACTCTCCGCAGGCGTTTGAGCTGCTTGTGGACGTCCTGCGCGGTTTCTACCGGCGTGTCAAAAAATTCGCGGAAAGTATCAGTGATCAATTCCGGGCGCGTATTCTGA  >  NC_000913/3387154‑3387415

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑