Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,660,262	T→C	V344A (GTC→GCC)	mdtE →	multidrug efflux pump membrane fusion protein MdtE

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,660,262	0	T	C	100.0%	220.6 / NA	61	V344A (GTC→GCC)	mdtE	multidrug efflux pump membrane fusion protein MdtE
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (33/28);  total (33/28)

TGGTGCCGCAGGAAGGCGTCACCCACAACGCCCAGGGTAAAGCAACGGCGCTCATTCTGGATAAAGACGATGTCGTGCAGCTACGCGAAATTGAAGCCAGCAAAGCCATCGGCGACCAGTGGGTCGTCACCTCTGGCTTGCAGGCTGGCGATCGGGTGATCGTTTCCGGTTTGCAACGCATTCGTCCGGGTATCAAAGCACGAGCAATTTCCTCCAGCCAGGAAAACGCCAGCACCGAATCGAAACAATAACGTTGCA  >  NC_000913/3660139‑3660396                                                                                                                            |                                                                                                                                       tGGTGCCGCAGGAAGGCGTCACCCACAACGCCCAGGGTAAAGCAACGGCGCTCATTCTGGATAAAGACGATGTCGTGCAGCTACGCGAAATTGAAGCCAGCAAAGCCATCGGCGACCAGTGGGCCGTCACCTCTGGCTTg                                                                                                                        <  1:85286/140‑1 (MQ=255) tGGTGCCGCAGGAAGGCGTCACCCACAACGCCCAGGGTAAAGCAACGGCGCTCATTCTGGATAAAGACGATGTCGTGCAGCTACGCGAAATTGAAGCCAGCAAAGCCATCGGCGACCAGTGGGCCGTCACCTCTGGCTTg                                                                                                                        >  2:689909/1‑140 (MQ=255)   gTGCCGCAGGAAGGCGTCACCCACAACGCCCAGGGTAAAGCAACGGCGCTCATTCTGGATAAAGACGATGTCGTGCAGCTACGCGAAATTGAAGCCAGCAAAGCCATCGGCGACCAGTGGgccgt                                                                                                                                     <  1:60718/125‑1 (MQ=255)   gTGCCGCAGGAAGGCGTCACCCACAACGCCCAGGGTAAAGCAACGGCGCTCATTCTGGATAAAGACGATGTCGTGCAGCTACGCGAAATTGAAGCCAGCAAAGCCATCGGCGACCAGTGGgccgt                                                                                                                                     >  2:60718/1‑125 (MQ=255)   gTGCCGCAGGAAGGCGTCACCCACAACGCCCAGGGTAAAGCAACGGCGCTCATTCTGGATAAAGACGATGTCGTGCAGCTACGCGAAATTGAAGCCAGCAAAGCCATCGGCGACCAGTGGGCCGTCACCTCTGGCTTGCa                                                                                                                      >  2:836981/1‑140 (MQ=255)   gTGCCGCAGGAAGGCGTCACCCACAACGCCCAGGGTAAAGCAACGGCGCTCATTCTGGATAAAGACGATGTCGTGCAGCTACGCGAAATTGAAGCCAGCAAAGCCATCGGCGACCAGTGGGCCGTCACCTCTGGCTTGCa                                                                                                                      >  2:613181/1‑140 (MQ=255)     gCCGCAGGAAGGCGTCACCCACAACGCCCAGGGTAAAGCAACGGCGCTCATTCTGGATAAAGACGATGTCGTGCAGCTACGCGAAATTGAAGCCAGCAAAGCCATCGGCGACCAGTGGGCCGTCACCTCTGGCTTGCAgg                                                                                                                    >  2:39637/1‑140 (MQ=255)     gCCGCAGGAAGGCGTCACCCACAACGCCCAGGGTAAAGCAACGGCGCTCATTCTGGATAAAGACGATGTCGTGCAGCTACGCGAAATTGAAGCCAGCAAAGCCATCGGCGACCAGTGGGCCGTCACCTCTGGCTTGCAgg                                                                                                                    >  1:495900/1‑140 (MQ=255)     gCCGCAGGAAGGCGTCACCCACAACGCCCAGGGTAAAGCAACGGCGCTCATTCTGGATAAAGACGATGTCGTGCAGCTACGCGAAATTGAAGCCAGCAAAGCCATCGGCGACCAGTGGGCCGTCACCTCTGGCTTGCAgg                                                                                                                    >  2:223028/1‑140 (MQ=255)      ccGCAGGAAGGCGTCACCCACAACGCCCAGGGTAAAGCAACGGCGCTCATTCTGGATAAAGACGATGTCGTGCAGCTACGCGAAATTGAAGCCAGCAAAGCCATCGGCGACCAGTGGGCCGTCACCTCTGGCTTGCAGGc                                                                                                                   <  1:779056/140‑1 (MQ=255)      ccGCAGGAAGGCGTCACCCACAACGCCCAGGGTAAAGCAACGGCGCTCATTCTGGATAAAGACGATGTCGTGCAGCTACGCGAAATTGAAGCCAGCAAAGCCATCGGCGACCAGTGGGCCGTCACCTCTGGCTTGCAGGc                                                                                                                   <  1:689909/140‑1 (MQ=255)          aGGAAGGCGTCACCCACAACGCCCAGGGTAAAGCAACGGCGCTCATTCTGGATAAAGACGATGTCGTGCAGCTACGCGAAATTGAAGCCAGCAAAGCCATCGGCGACCAGTGGGCCGTCCCCTCTGGCTTGCAGGCTGGc                                                                                                               <  1:236668/140‑1 (MQ=255)                   tCACCCACAACGCCCAGGGTAAAGCAACGGCGCTCATTCTGGATAAAGACGATGTCGTGCAGCTACGCGAAATTGAAGCCAGCAAAGCCATCGGCGACCAGTGGGCCGTCACCTCTGGCTTGCAGGCTGGCGATCGGGTg                                                                                                      >  1:572964/1‑140 (MQ=255)                      cccACAACGCCCAGGGTAAAGCAACGGCGCTCATTCTGGATAAAGACGATGTCGTGCAGCTACGCGAAATTGAAGCCAGCAAAGCCATCGGCGACCAGTGGGCCGTCACCTCTGGCTTGCAGGCTGGCGATCGGGTGATc                                                                                                   >  1:288690/1‑140 (MQ=255)                          caACGCCCAGGGTAAAGCAACGGCGCTCATTCTGGATAAAGACGATGTCGTGCAGCTACGCGAAATTGAAGCCAGCAAAGCCATCGGCGACCAGTGGGCCGTCACCTCTGGCTTGCAGGCTGGCGATCGGGTGATCGttt                                                                                               >  2:1047227/1‑140 (MQ=255)                            aCGCCCAGGGTAAAGCAACGGCGCTCATTCTGGATAAAGACGATGTCGTGCAGCTACGCGAAATTGAAGCCAGCAAAGCCATCGGCGACCAGTGGGCCGTCACCTCTGGCTTGCAGGCTGGCGATCGGGTGATCGTTTcc                                                                                             >  1:581970/1‑140 (MQ=255)                            aCGCCCAGGGTAAAGCAACGGCGCTCATTCTGGATAAAGACGATGTCGTGCAGCTACGCGAAATTGAAGCCAGCAAAGCCATCGGCGACCAGTGGGCCGTCACCTCTGGCTTGCAGGCTGGCGATCGGGTGATCGTTTcc                                                                                             <  2:495900/140‑1 (MQ=255)                                ccAGGGTAAAGCAACGGCGCTCATTCTGGATAAAGACGATGTCGTGCAGCTACGCGAAATTGAAGCCAGCAAAGCCATCGGCGACCAGTGGGCCGTCACCTCTGGCTTGCAGGCTGGCGATCGGGTGATCGTTTCCGGtt                                                                                         >  2:882672/1‑140 (MQ=255)                                ccAGGGTAAAGCAACGGCGCTCATTCTGGATAAAGACGATGTCGTGCAGCTACGCGAAATTGAAGCCAGCAAAGCCATCGGCGACCAGTGGGCCGTCACCTCTGGCTTGCAGGCTGGCGATCGGGTGATCGTTTCCGGtt                                                                                         >  2:548234/1‑140 (MQ=255)                                 cAGGGTAAAGCAACGGCGCTCATTCTGGATAAAGACGATGTCGTGCAGCTACGCGAAATTGAAGCCAGCAAAGCCATCGGCGACCAGTGGGCCGTCACCTCTGGCTTGCAGGCTGGCGATCGGGTGATCGTTTCCGGttt                                                                                        >  1:158269/1‑140 (MQ=255)                                         aGCAACGGCGCTCATTCTGGATAAAGACGATGTCGTGCAGCTACGCGAAATTGAAGCCAGCAAAGCCATCGGCGACCAGTGGGCCGTCACCTCTGGCTTGCAGGCTGGCGATCGGGTGATCGTTTCCGGttt                                                                                        >  2:672422/1‑132 (MQ=255)                                         aGCAACGGCGCTCATTCTGGATAAAGACGATGTCGTGCAGCTACGCGAAATTGAAGCCAGCAAAGCCATCGGCGACCAGTGGGCCGTCACCTCTGGCTTGCAGGCTGGCGATCGGGTGATCGTTTCCGGttt                                                                                        <  1:672422/132‑1 (MQ=255)                                         aGCAACGGCGCTCATTCTGGATAAAGACGATGTCGTGCAGCTACGCGAAATTGAAGCCAGCAAAGCCATCGGCGACCAGTGGGCCGTCACCTCTGGCTTGCAGGCTGGCGATCGGGTGATCGTTTCCGGTTTGCAACGCa                                                                                >  2:1019236/1‑140 (MQ=255)                                            aaCGGCGCTCATTCTGGATAAAGACGATGTCGTGCAGCTACGCGAAATTGAAGCCAGCAAAGCCATCGGCGACCAGTGGGCCGTCACCTCTGGCTTGCAGGCTGGCGATCGGGTGATCGTTTCCGGTTTGCAACGCATTc                                                                             <  2:200833/140‑1 (MQ=255)                                              cGGCGCTCATTCTGGATAAAGACGATGTCGTGCAGCTACGCGAAATTGAAGCCAGCAAAGCCATCGGCGACCAGTGGGCCGTCACCTCTGGCTTGCAGGCTGGCGATCGGGTGATCGTTTCCGGtt                                                                                         <  2:812982/126‑1 (MQ=255)                                              cGGCGCTCATTCTGGATAAAGACGATGTCGTGCAGCTACGCGAAATTGAAGCCAGCAAAGCCATCGGCGACCAGTGGGCCGTCACCTCTGGCTTGCAGGCTGGCGATCGGGTGATCGTTTCCGGtt                                                                                         >  1:812982/1‑126 (MQ=255)                                              cGGCGCTCATTCTGGATAAAGACGATGTCGTGCAGCTACGCGAAATTGAAGCCAGCAAAGCCATCGGCGACCAGTGGGCCGTCACCTCTGGCTTGCAGGCTGGCGATCGGGTGATCGTTTCCGGTTTGCAACGCATTCGt                                                                           >  2:5141/1‑140 (MQ=255)                                              cGGCGCTCATTCTGGATAAAGACGATGTCGTGCAGCTACGCGAAATTGAAGCCAGCAAAGCCATCGGCGACCAGTGGGCCGTCACCTCTGGCTTGCAGGCTGGCGATCGGGTGATCGTTTCCGGTTTGCAACGCATTCGt                                                                           >  2:938244/1‑140 (MQ=255)                                              cGGCGCTCATTCTGGATAAAGACGATGTCGTGCAGCTACGCGAAATTGAAGCCAGCAAAGCCATCGGCGACCAGTGGGCCGTCACCTCTGGCTTGCAGGCTGGCGATCGGGTGATCGTTTCCGGTTTGCAACGCATTCGt                                                                           >  1:838671/1‑140 (MQ=255)                                              cGGCGCTCATTCTGGATAAAGACGATGTCGTGCAGCTACGCGAAATTGAAGCCAGCAAAGCCATCGGCGACCAGTGGGCCGTCACCTCTGGCTTGCAGGCTGGCGATCGGGTGATCGTTTCCGGTTTGCAACGCATTCGt                                                                           <  1:524669/140‑1 (MQ=255)                                              cGGCGCTCATTCTGGATAAAGACGATGTCGTGCAGCTACGCGAAATTGAAGCCAGCAAAGCCATCGGCGACCAGTGGGCCGTCACCTCTGGCTTGCAGGCTGGCGATCGGGTGATCGTTTCCGGTTTGCAACGCATTCGt                                                                           <  2:838671/140‑1 (MQ=255)                                                 cgcTCATTCTGGATAAAGACGATGTCGTGCAGCTACGCGAAATTGAAGCCAGCAAAGCCATCGGCGACCAGTGGGCCGTCACCTCTGGCTTGCAGGCTGGCGATCGGGTGATCGTTTCCGGTTTGCAACGCATTCGTCCg                                                                        <  2:581970/140‑1 (MQ=255)                                                  gcTCATTCTGGATAAAGACGATGTCGTGCAGCTACGCGAAATTGAAGCCAGCAAAGCCATCGGCGACCAGTGGGCCGTCACCTCTGGCTTGCAGGCTGGCGATCGGGTGATCGTTTCCGGTTTGCAACGCATTCGTCCgg                                                                       <  1:280415/140‑1 (MQ=255)                                                  gcTCATTCTGGATAAAGACGATGTCGTGCAGCTACGCGAAATTGAAGCCAGCAAAGCCATCGGCGACCAGTGGGCCGTCACCTCTGGCTTGCAGGCTGGCGATCGGGTGATCGTTTCCGGTTTGCAACGCATTCGTCCgg                                                                       <  2:288690/140‑1 (MQ=255)                                                        tCTGGATAAAGACGATGTCGTGCAGCTACGCGAAATTGAAGCCAGCAAAGCCATCGGCGACCAGTGGGCCGTCACCTCTGGCTTGCAGGCTGGCGATCGGGTGATCGTTTCCGGTTTGCAACGCATTCGTCCGGGTATCa                                                                 >  2:519514/1‑140 (MQ=255)                                                              tAAAGACGATGTCGTGCAGCTACGCGAAATTGAAGCCAGCAAAGCCATCGGCGACCAGTGGGCCGTCACCTCTGGCTTGCAGGCTGGCGATCTGGTGATCGTTTCCGGCTTGCAACGCATTCGTCCGGGTATCAAAGCAc                                                           >  2:188796/1‑140 (MQ=255)                                                                 aGACGATGTCGTGCAGCTACGCGAAATTGAAGCCAGCAAAGCCATCGGCGACCAGTGGGCCGTCACCTCTGGCTTGCAGGCTGGCGATCGGGTGATCGTTTCCGGTTTGCAACGCATTCGTCCGGGTATCAAAGCACGAg                                                        <  1:39637/140‑1 (MQ=255)                                                                  gACGATGTCGTGCAGCTACGCGAAATTGAAGCCAGCAAAGCCATCGGCGACCAGTGGGCCGTCACCTCTGGCTTGCAGGCTGGCGATCGGGTGATCGTTTCCGGTTTGCAACGCATTCGTCCGGGTATCAAAGCACGAGc                                                       >  2:1005900/1‑140 (MQ=255)                                                                     gATGTCGTGCAGCTACGCGAAATTGAAGCCAGCAAAGCCATCGGCGACCAGTGGGCCGTCACCTCTGGCTTGCAGGCTGGCGATCGGGTGATCGTTTCCGGTTTGCAACGCATTCGTCCGGGTATCAAAGCACGAGCAAt                                                    <  1:882672/140‑1 (MQ=255)                                                                       tGTCGTGCAGCTACGCGAAATTGAAGCCAGCAAAGCCATCGGCGACCAGTGGGCCGTCACCTCTGGCTTGCAGGCTGGCGATCGGGTGATCGTTTCCGGTTTGCAACGCATTCGTCCGGGTATCAAAGCACGAGCAAttt                                                  >  2:78202/1‑140 (MQ=255)                                                                          cGTGCAGCTACGCGAAATTGAAGCCAGCAAAGCCATCGGCGACCAGTGGGCCGTCACCTCTGGCTTGCAGGCTGGCGATCGGGTGATCGTTTCCGGTTTGCAACGCATTCGTCCGGGTATCAAAGCACGAGCAATTtcct                                               <  1:938244/140‑1 (MQ=255)                                                                          cGTGCAGCTACGCGAAATTGAAGCCAGCAAAGCCATCGGCGACCAGTGGGCCGTCACCTCTGGCTTGCAGGCTGGCGATCGGGTGATCGTTTCCGGTTTGCAACGCATTCGTCCGGGTATCAAAGCACGAGCAATTtcct                                               <  1:1005900/140‑1 (MQ=255)                                                                           gTGCAGCTACGCGAAATTGAAGCCAGCAAAGCCATCGGCGACCAGTGGGCCGTCACCTCTGGCTTGCAGGCTGGCGATCGGGTGATCGTTTCCGGTTTGCAACGCATTCGTCCGGGTATCAAAGCACGAGCAATTtcctc                                              >  2:870036/1‑140 (MQ=255)                                                                            tGCAGCTACGCGAAATTGAAGCCAGCAAAGCCATCGGCGACCAGTGGGCCGTCACCTCTGGCTTGCAGGCTGGCGATCGGGTGATCGTTTCCGGTTTGCAACGCATTCGTCCGGGTATCAAAGCACGAGCAATTtcctcc                                             >  2:806775/1‑140 (MQ=255)                                                                             gCAGCTACGCGAAATTGAAGCCAGCAAAGCCATCGGCGACCAGTGGGCCGTCACCTCTGGCTTGCAGGCTGGCGATCGGGTGATCGTTTCCGGTTTGCAACGCATTCGTCCGGGTATCAAAGCACGAGCAATTTCCTcca                                            >  1:709691/1‑140 (MQ=255)                                                                              cAGCTACGCGAAATTGAAGCCAGCAAAGCCATCGGCGACCAGTGGGCCGTCACCTCTGGCTTGCAGGCTGGCGATCGGGTGATCGTTTCCGGTTTGCAACGCATTCGTCCGGGTATCAAAGCACGAGCAATTTCCTccag                                           <  1:1033906/140‑1 (MQ=255)                                                                                 cTACGCGAAATTGAAGCCAGCAAAGCCATCGGCGACCAGTGGGCCGTCACCTCTGGCTTGCAGGCTGGCGATCGGGTGATCGTTTCCGGTTTGCAACGCATTCGTCCGGGTATCAAAGCACGAGCAATTTCCTccagcca                                        >  1:595548/1‑140 (MQ=255)                                                                                 cTACGCGAAATTGAAGCCAGCAAAGCCATCGGCGACCAGTGGGCCGTCACCTCTGGCTTGCAGGCTGGCGATCGGGTGATCGTTTCCGGTTTGCAACGCATTCGTCCGGGTATCAAAGCACGAGCAATTTCCTccagcca                                        >  2:783215/1‑140 (MQ=255)                                                                                 cTACGCGAAATTGAAGCCAGCAAAGCCATCGGCGACCAGTGGGCCGTCACCTCTGGCTTGCAGGCTGGCGATCGGGTGATCGTTTCCGGTTTGCAACGCATTCGTCCGGGTATCAAAGCACGAGCAATTTCCTccagcca                                        >  1:151365/1‑140 (MQ=255)                                                                                   aCGCGAAATTGAAGCCAGCAAAGCCATCGGCGACCAGTGGGCCGTCACCTCTGGCTTGCAGGCTGGCGATCGGGTGATCGTTTCCGGTTTGCAACGCATTCGTCCGGGTATCAAAGCACGAGCAATTTCCTCCAGCCAgg                                      >  1:1047593/1‑140 (MQ=255)                                                                                     gcgAAATTGAAGCCAGCAAAGCCATCGGCGACCAGTGGGCCGTCACCTCTGGCTTGCAGGCTGGCGATCGGGTGATCGTTTCCGGTTTGCAACGCATTCGTCCGGGTATCAAAGCACGAGCAATTTCCTCCAGCCAGGaa                                    <  2:595548/140‑1 (MQ=255)                                                                                     gcgAAATTGAAGCCAGCAAAGCCATCGGCGACCAGTGGGCCGTCACCTCTGGCTTGCAGGCTGGCGATCGGGTGATCGTTTCCGGTTTGCAACGCATTCGGCCGGGTATCAAAGCACGAGCAATTTCCTCCAGCCAGGaa                                    <  1:548234/140‑1 (MQ=255)                                                                                                   aGCAAAGCCATCGGCGACCAGTGGGCCGTCACCTCTGGCTTGCAGGCTGGCGATCGGGTGATCGTTTCCGGTTTGCAACGCATTCGTCCGGGTATCAAAGCACGAGCAATTTCCTCCAGCCAGGAAAACGCCAGCACCGa                      >  1:310974/1‑140 (MQ=255)                                                                                                     cAAAGCCATCGGCGACCAGTGGGCCGTCACCTCTGGCTTGCAGGCTGGCGATCGGGTGATCGTTTCCGGTTTGCAACGCATTCGTCCGGGTATCAAAGCACGAGCAATTTCCTCCAGCCAGGAAAACGCCAGCACCGAAt                    >  1:485608/1‑140 (MQ=255)                                                                                                        aGCCATCGGCGACCAGTGGGCCGTCACCTCTGGCTTGCAGGCTGGCGATCGGGTGATCGTTTCCGGTTTGCAACGCATTCGTCCGGGTATCAAAGCACGAGCAATTTCCTCCAGCCAGGAAAACGCCAGCACCGAATCGa                 <  2:485608/140‑1 (MQ=255)                                                                                                             tCGGCGACCAGTGGGCCGTCACCTCTGGCTTGCAGGCTGGCGATCGGGTGATCGTTTCCGGTTTGCAACGCATTCGTCCGGGTATCAAAGCACGAGCAATTTCCTCCAGCCAGGAAAACGCCAGCACCGAATCGAAACaa            <  1:1019236/140‑1 (MQ=255)                                                                                                              cGGCGACCAGTGGGCCGTCACCTCTGGCTTGCAGGCTGGCGATCGGGTGATCGTTTCCGGTTTGCAACGCATTCGTCCGGGTATCAAAGCACGAGCAATTTCCTCCAGCCAGGAAAACGCCAGCACCGAATCg                  >  2:1024422/1‑133 (MQ=255)                                                                                                              cGGCGACCAGTGGGCCGTCACCTCTGGCTTGCAGGCTGGCGATCGGGTGATCGTTTCCGGTTTGCAACGCATTCGTCCGGGTATCAAAGCACGAGCAATTTCCTCCAGCCAGGAAAACGCCAGCACCGAATCg                  <  1:1024422/133‑1 (MQ=255)                                                                                                                gCGACCAGTGGGCCGTCACCTCTGGCTTGCAGGCTGGCGATCGGGTGATCGTTTCCGGTTTGCAACGCATTCGTCCGGGTATCAAAGCACGAGCAATTTCCTCCAGCCAGGAAAACGCCAGCACCGAATCGAAACAATaa         <  2:310974/140‑1 (MQ=255)                                                                                                                  gACCAGTGGGCCGTCACCTCTGGCTTGCAGGCTGGCGATCGGGTGATCGTTTCCGGTTTGCAACGCATTCGTCCGGGTATCAAAGCACGAGCAATTTCCTCCAGCCAGGAAAACGCCAGCACCGAATCGAAACAATAACg       <  2:709691/140‑1 (MQ=255)                                                                                                                     cAGTGGGCCGTCACCTCTGGCTTGCAGGCTGGCGATCGGGTGATCGTTTCCGGTTTGCAACGCATTCGTCCGGGTATCAAAGCACGAGCAATTTCCTCCAGCCAGGAAAACGCCAGCACCGAATCGAAACAATAACGTTg    <  1:1047227/140‑1 (MQ=255)                                                                                                                      aGTGGGCCGTCACCTCTGGCTTGCAGGCTGGCGATCGGGTGATCGTTTCCGGTTTGCAACGCATTCGTCCGGGTATCAAAGCACGAGCAATTTCCTCCAGCCAGGAAAACGCCAGCACCGAATCGAAACAATAACGTTGc   <  1:78202/140‑1 (MQ=255)                                                                                                                       gTGGGCCGTCACCTCTGGCTTGCAGGCTGGCGATCGGGTGATCGTTTCCGGTTTGCAACGCATTCGTCCGGGTATCAAAGCACGAGCAATTTCCTCCAGCCAGGAAAACGCCAGCACCGAATCGAAACAATAACGTTGCa  <  1:5141/140‑1 (MQ=255)                                                                                                                            |                                                                                                                                       TGGTGCCGCAGGAAGGCGTCACCCACAACGCCCAGGGTAAAGCAACGGCGCTCATTCTGGATAAAGACGATGTCGTGCAGCTACGCGAAATTGAAGCCAGCAAAGCCATCGGCGACCAGTGGGTCGTCACCTCTGGCTTGCAGGCTGGCGATCGGGTGATCGTTTCCGGTTTGCAACGCATTCGTCCGGGTATCAAAGCACGAGCAATTTCCTCCAGCCAGGAAAACGCCAGCACCGAATCGAAACAATAACGTTGCA  >  NC_000913/3660139‑3660396

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑