Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,695,809	(A)6→5	coding (9/1557 nt)	mltF →	membrane‑bound lytic murein transglycosylase F

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,695,804	0	A	.	100.0%	166.0 / NA	39	coding (4/1557 nt)	mltF	membrane‑bound lytic murein transglycosylase F
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base . (23/16);  total (23/16)

CCGCCAATGGCTGTAATAAGTTGCCATCTGGCGCAGGTTTACGCAAAATGCCGCTCATTTATGAGTAAACCTTTCACTATTATTACGTTTTTTCAAGCTGGGACGCGCACGACACAGAGAATTAACTAATTGAAAAAATTAAAGATTAATTATCTGTTCATCGGCATTCTGGCACTGCTGCTCGCGGTCGCTCTCTGGCCATCCATTCCCTGGTTTGGTAAAGCCGACAACCGTATCGCCGCCATTCAAGCGCGGGGAGAGTTG  >  NC_000913/2695672‑2695935                                                                                                                                     |                                                                                                                                    ccgccAATGGCTGTAATAAGTTGCCATCTGGCGCAGGTTTACGCAAAATGCCGCTCATTTATGAGTAAACCTTTCACTATTATTACGTTTTTTCAAGCTGGGACGCGCACGACACAGAGAATTAACTAATTGaaaaatta                                                                                                                              >  2:180046/1‑137 (MQ=255)        tGGCTGTAATAAGTTGCCATCTGGCGCAGGTTTACGCAAAATGCCGCTCATTTATGAGTAAACCTTTCACTATTATTACGTTTTTTCAAGCTGGGACGCGCACGACACAGAGAATTAACTAATTG‑AAAAATTAAAGatta                                                                                                                      >  2:96157/1‑140 (MQ=255)                    gTTGCCATCTGGCGCAGGTTTACGCAAAATGCCGCTCATTTATGAGTAAACCTTTCACTATTATTACGTTTTTTCAAGCTGGGACGCGCACGACACAGAGAATTAACTAATTG‑AAAAATTAAAGATTAATTATCTGTTCa                                                                                                          >  1:852508/1‑140 (MQ=255)                     ttGCCATCTGGCGCAGGTTTACGCAAAATGCCGCTCATTTATGAGTAAACCTTTCACTATTATTACGTTTTTTCAAGCTGGGACGCGCACGACACAGAGAATTAACTAATTG‑AAAAATTAAAGATTAATTATCTGTTCAt                                                                                                         >  2:235715/1‑140 (MQ=255)                     ttGCCATCTGGCGCAGGTTTACGCAAAATGCCGCTCATTTATGAGTAAACCTTTCACTATTATTACGTTTTTTCAAGCTGGGACGCGCACGACACAGAGAATTAACTAATTG‑AAAAATTAAAGATTAATTATCTGTTCAt                                                                                                         >  1:13284/1‑140 (MQ=255)                        ccATCTGGCGCAGGTTTACGCAAAATGCCGCTCATTTATGAGTAAACCTTTCACTATTATTACGTTTTTTCAAGCTGGGACGCGCACGACACAGAGAATTAACTAATTG‑AAAAATTAAAGATTAATTATCTGTTCATCgg                                                                                                      <  2:323148/140‑1 (MQ=255)                        ccATCTGGCGCAGGTTTACGCAAAATGCCGCTCATTTATGAGTAAACCTTTCACTATTATTACGTTTTTTCAAGCTGGGACGCGCACGACACAGAGAATTAACTAATTG‑AAAAATTAAAGATTAATTATCTGTTCATCgg                                                                                                      <  1:756492/140‑1 (MQ=255)                               gcgcAGGTTTACGCAAAATGCCGCTCATTTATGAGTAAACCTTTCACTATTATTACGTTTTTTCAAGCTGGGACGCGCACGACACAGAGAATTAACTAATTG‑AAAAATTAAAGATTAATTATCTGTTCATCGGCATTCTg                                                                                               >  2:867107/1‑140 (MQ=255)                                                     gCTCATTTATGAGTAAACCTTTCACTATTATTACGTTTTTTCAAGCTGGGACGCGCACGACACAGAGAATTAACTAATTG‑AAAAATTAAAGATTAATTATCTGTTCATCGGCATTCTGGCACTGCTGCTCGCGGt                                                                              >  1:708147/1‑135 (MQ=255)                                                     gCTCATTTATGAGTAAACCTTTCACTATTATTACGTTTTTTCAAGCTGGGACGCGCACGACACAGAGAATTAACTAATTG‑AAAAATTAAAGATTAATTATCTGTTCATCGGCATTCTGGCACTGCTGCTCGCGGt                                                                              <  2:708147/135‑1 (MQ=255)                                                      cTCATTTATGAGTAAACCTTTCACTATTATTACGTTTTTTCAAGCTGGGACGCGCACGACACAGAGAATTAACTCATTG‑AAAAATTAAAGATTAATTATCTGTTCATCGGCATTCTGGCACTGCTGCTCGCGGTCGctct                                                                        <  2:852508/140‑1 (MQ=255)                                                                     aCCTTTCACTATTATTACGTTTTTTCAAGCTGGGACGCGCACGACACAGAGAATTAACTAATTG‑AAAAATTAAAGATTAATTATCTGTTCATCGGCATTCTGGCACTGCTGCTCGCGGTCGCTCTCTGGccat                                                                >  1:544736/1‑133 (MQ=255)                                                                     aCCTTTCACTATTATTACGTTTTTTCAAGCTGGGACGCGCACGACACAGAGAATTAACTAATTG‑AAAAATTAAAGATTAATTATCTGTTCATCGGCATTCTGGCACTGCTGCTCGCGGTCGCTCTCTGGccat                                                                <  2:544736/133‑1 (MQ=255)                                                                         ttCACTATTATTACGTTTTTTCAAGCTGGGACGCGCACGACACAGAGAATTAACTAATTG‑AAAAATTAAAGATTAATTATCTGTTCATCGGCATTCTGGCACTGCTGCTCGCGGTCGCTCTCTGGCCATCCATTCCCTgg                                                     <  1:96157/140‑1 (MQ=255)                                                                           cACTATTATTACGTTTTTTCAAGCTGGGACGCGCACGACACAGAGAATTAACTAATTG‑AAAAATTAAAGATTAATTATCTGTTCATCGGCATTCTGGCACTGCTGCTCGCGGTCGCTCTCTGGCCATCCATTCCCTGGtt                                                   >  2:774323/1‑140 (MQ=255)                                                                                ttattaCGTTTTTTCAAGCTGGGACGCGCACGACACAGAGAATTAACTAATTG‑AAAAATTAAAGATTAATTATCTGTTCATCGGCATTCTGGCACTGCTGCTCGCGGTCGCTCTCTGGCCATCCATTCCCTGGTTTGGTa                                              >  1:367212/1‑140 (MQ=255)                                                                                 tattaCGTTTTTTCAAGCTGGGACGCGCACGACACAGAGAATTAACTAATTG‑AAAAATTAAAGATTAATTATCTGTTCATCGGCATTCTGGCACTGCTGCTCGCGGTCGCTCTCTGGCCATCCATTCCCTGGTTTGGTaa                                             >  1:149346/1‑140 (MQ=255)                                                                                   ttaCGTTTTTTCAAGCTGGGACGCGCACGACACAGAGAATTAACTAATTG‑AAAAATTAAAGATTAATTATCTGTTCATCGGCATTCTGGCACTGCTGCTCGCGGTCGCTCTCTGTCCATCCATTCCCTGGTTTGGTAAAg                                           >  2:261982/1‑140 (MQ=255)                                                                                   ttaCGTTTTTTCAAGCTGGGACGCGCACGACACAGAGAATTAACTAATTG‑AAAAATTAAAGATTAATTATCTGTTCATCGGCATTCTGGCACTGCTGCTCGCGGTCGCTCTCTGGCCATCCATTCCCTGGTTTGGTAAAg                                           >  1:617571/1‑140 (MQ=255)                                                                                       gTTTTTTCAAGCTGGGACGCGCACGACACAGAGAATTAACTAATTG‑AAAAATTAAAGATTAATTATCTGTTCATCGGCATTCTGGCACTGCTGCTCGCGGTCGCTCTCTGGCCATCCATTCCCTGGTTTGGTAAAGCCGa                                       <  1:235715/140‑1 (MQ=255)                                                                                        ttttttCAAGCTGGGACGCGCACGACACAGAGAATTAACTAATTG‑AAAAATTAAAGATTAATTATCTGTTCATCGGCATTCTGGCACTGCTGCTCGCGGTCGCTCTCTGGCCATCCATTCCCTGGTTTGGTAAAGCCGAc                                      >  2:791685/1‑140 (MQ=255)                                                                                          ttttCAAGCTGGGACGCGCACGACACAGAGAATTAACTAATTG‑AAAAATTAAAGATTAATTATCTGTTCATCGGCATTCTGGCACTGCTGCTCGCGGTCGCTCTCTGGCCATCCATTCCCTGGTTTGGTAAAGCCGACaa                                    >  1:409203/1‑140 (MQ=255)                                                                                            ttCAAGCTGGGACGCGCACGACACAGAGAATTAACTAATTG‑AAAAATTAAAGATTAATTATCTGTTCATCGGCATTCTGGCACTGCTGCTCGCGGTCGCTCTCTGGCCATCCATTCCCTGGTTTGGTAAAGCCGACAAcc                                  >  2:757849/1‑140 (MQ=255)                                                                                                   tGGGACGCGCACGACACAGAGAATTAACTAATTG‑AAAAATTAAAGATTAATTATCTGTTCATCGGCATTCTGGCACTGCTGCTCGCGGTCGCTCTCTGGCCATCCATTCCCTGGTTTGGTAAAGCCGACAACCGTATcgc                           <  2:617571/140‑1 (MQ=255)                                                                                                     ggACGCGCACGACACAGAGAATTAACTAATTG‑AAAAATTAAAGATTAATTATCTGTTCATCGGCATTCTGGCACTGCTGCTCGCGGTCGCTCTCTGGCCATCCATTCCCTGGTTTGGTAAAGCCGACAACCGTATcgccg                         >  2:220088/1‑140 (MQ=255)                                                                                                          cgcACGACACAGAGAATTAACTAATTG‑AAAAATTAAAGATTAATTATCTGTTCATCGGCATTCTGGCACTGCTGCTCGCGGTCGCTCTCTGGCCATCCATTCCCTGGTTTGGTAAAGCCGACAACCGTATCGCCGCCAtt                    >  1:812562/1‑140 (MQ=255)                                                                                                          cgcACGACACAGAGAATTAACTAATTG‑AAAAATTAAAGATTAATTATCTGTTCATCGGCATTCTGGCACTGCTGCTCGCGGTCGCTCTCTGGCCATCCATTCCCTGGTTTGGTAAAGCCGACAACCGTATCGCCGCCAtt                    <  1:774323/140‑1 (MQ=255)                                                                                                           gcACGACACAGAGAATTAACTAATTG‑AAAAATTAAAGATTAATTATCTGTTCATCGGCATTCTGGCACTGCTGCTCGCGGTCGCTCTCTGGCCATCCATTCCCTGGTTTGGTAAAGCCGACAACCGTATcgccg                         >  1:785816/1‑134 (MQ=255)                                                                                                           gcACGACACAGAGAATTAACTAATTG‑AAAAATTAAAGATTAATTATCTGTTCATCGGCATTCTGGCACTGCTGCTCGCGGTCGCTCTCTGGCCATCCATTCCCTGGTTTGGTAAAGCCGACAACCGTATcgccg                         <  2:785816/134‑1 (MQ=255)                                                                                                              cGACACAGAGAATTAACTAATTG‑AAAAATTAAAGATTAATTATCTGTTCATCGGCATTCTGGCACTGCTGCTCGCGGTCGCTCTCTGGCCATCCATTCCCTGGTTTGGTAAAGCCGACAACCGTATCGCCGCCATTCAAg                <  2:367212/140‑1 (MQ=255)                                                                                                               gACACAGAGAATTAACTAATTG‑AAAAATTAAAGATTAATTATCTGTTCATCGGCATTCTGGCACTGCTGCTCGCGGTCGCTCTCTGGCCATCCATTCCCTGGTTTGGTAAAGCCGACAACCGTATCGCCGCCATTCa                  <  1:780621/137‑1 (MQ=255)                                                                                                               gACACAGAGAATTAACTAATTG‑AAAAATTAAAGATTAATTATCTGTTCATCGGCATTCTGGCACTGCTGCTCGCGGTCGCTCTCTGGCCATCCATTCCCTGGTTTGGTAAAGCCGACAACCGTATCGCCGCCATTCa                  >  2:780621/1‑137 (MQ=255)                                                                                                               gACACAGAGAATTAACTAATTG‑AAAAATTAAAGATTAATTATCTGTTCATCGGCATTCTGGCACTGCTGCTCGCGGTCGCTCTCTGGCCATCCATTCCCTGGTTTGGTAAAGCCGACAACCGTATCGCCGCCATTCAAgc               <  2:560874/140‑1 (MQ=255)                                                                                                               gACACAGAGAATTAACTAATTG‑AAAAATTAAAGATTAATTATCTGTTCATCGGCATTCTGGCACTGCTGCTCGCGGTCGCTCTCTGGCCATCCATTCCCTGGTTTGGTAAAGCCGACAACCGTATCGCCGCCATTCAAgc               <  1:757849/140‑1 (MQ=255)                                                                                                                     gagaATTAACTAATTG‑AAAAATTAAAGATTAATTATCTGTTCATCGGCATTCTGGCACTGCTGCTCGCGGTCGCTCTCTGGCCATCCATTCCCTGGTTTGGTAAAGCCGACAACCGTATCGCCGCCATTCAAGCGCgggg         >  1:397188/1‑140 (MQ=255)                                                                                                                         aTTAACTAATTG‑AAAAATTAAAGATTAATTATCTGTTCATCGGCATTCTGGCACTGCTGCTCGCGGTCGCTCTCTGGCCATCCATTCCCTGGTTTGGTAAAGCCGACAACCGTATCGCCGCCATTCAAGCGCGGGGagag     >  1:289754/1‑140 (MQ=255)                                                                                                                           tAACTAATTG‑AAAAATTAAAGATTAATTATCTGTTCATCGGCATTCTGGCACTGCTGCTCGCGGTCGCTCTCTGGCCATCCATTCCCTGGTTTGGTAAAGCCGACAACCGTATCGCCGCCATTCAAGCGCGGGGAGAGtt   >  2:626791/1‑140 (MQ=255)                                                                                                                           tAACTAATTG‑AAAAATTAAAGATTAATTATCTGTTCATCGGCATTCTGGCACTGCTGCTCGCGGTCGCTCTCTGGCCATCCATTCCCTGGTTTGGTAAAGCCGACAACCGTATCGCCGCCATTCAAGCGCGGGGAGAGtt   >  1:113018/1‑140 (MQ=255)                                                                                                                            aaCTAATTG‑AAAAATTAAAGATTAATTATCTGTTCATCGGCATTCTGGCACTGCTGCTCGCGGTCGCTCTCTGGCCATCCATTCCCTGGTTTGGTAAAGCCGACAACCGTATCGCCGCCATTCAAGCGCGGGGAGAGTTg  <  2:113018/140‑1 (MQ=255)                                                                                                                            aaCTAATTG‑AAAAATTAAAGATTAATTATCTGTTCATCGGCATTCTGGCACTGCTGCTCGCGGTCGCTCTCTGGCCATCCATTCCCTGGTTTGGTAAAGCCGACAACCGTATCGCCGCCATTCAAGCGCGGGGAGAGTTg  <  2:812562/140‑1 (MQ=255)                                                                                                                                     |                                                                                                                                    CCGCCAATGGCTGTAATAAGTTGCCATCTGGCGCAGGTTTACGCAAAATGCCGCTCATTTATGAGTAAACCTTTCACTATTATTACGTTTTTTCAAGCTGGGACGCGCACGACACAGAGAATTAACTAATTGAAAAAATTAAAGATTAATTATCTGTTCATCGGCATTCTGGCACTGCTGCTCGCGGTCGCTCTCTGGCCATCCATTCCCTGGTTTGGTAAAGCCGACAACCGTATCGCCGCCATTCAAGCGCGGGGAGAGTTG  >  NC_000913/2695672‑2695935

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑