Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,565,981	(T)7→8	coding (601/2448 nt)	glgP ←	glycogen phosphorylase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,565,974	1	.	T	100.0%	183.8 / NA	52	T203K (ACG→AAG)	glgP	glycogen phosphorylase
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base T (29/23);  total (29/23)

CTGGTTGAATTTACCGAGGTTAATTTCGCTACTGGCTTGCGCACTCCACAAACGCAGCGTGTTGGTCGCGTCGGTGTCGTAACCAGGGATTATCTGATCGTAAGCGACTCCCAGAATCTCTTCGGTTTCAATCCAGCGCG‑TTTTTTTACCTTCCTGCTGAATGCGACCGCCAAAACGGACTTTATAGCGCGTGTTGTGGCGTTTGAATTCCCACGGGTTACCGTATTCCAGCCAGTAGTCTGGCGACTCTTTCTGGCTACCGTTAACGATGT  >  NC_000913/3565835‑3566106                                                                                                                                             |                                                                                                                                     cTGGTTGAATTTACCGAGGTTAATTTCGCTACTGGCTTGCGCACTCCACAAACGCAGCGTGTTGGTCGCGTCGGTGTCGTAACCAGGGATTATCTGATCGTAAGCGACTCCCAGAATCTCTTCGGTTTCAATCCAGcgcg                                                                                                                                       <  1:654518/140‑1 (MQ=255) cTGGTTGAATTTACCGAGGTTAATTTCGCTACTGGCTTGCGCACTCCACAAACGCAGCGTGTTGGTCGCGTCGGTGTCGTAACCAGGGATTATCTGATCGTAAGCGACTCCCAGAATCTCTTCGGTTTCAATCCAGcgcg                                                                                                                                       >  1:552102/1‑140 (MQ=255)   ggTTGAATTTACCGAGGTTAATTTCGCTACTGGCTTGCGCACTCCACAAACGCAGCGTGTTGGTCGCGTCGGTGTCGTAACCAGGGATTATCTGATCGTAAGCGACTCCCAGAATCTCTTCGGTTTCAATCCAGCGCG‑tt                                                                                                                                    >  1:471055/1‑140 (MQ=255)          tttACCGAGGTTAATTTCGCTACTGGCTTGCGCACTCCACAAACGCAGCGTGTTGGTCGCGTCGGTGTCGTAACCAGGGATTATCTGATCGTAAGCGACTCCCAGAATCTCTTCGGTTTCAATCCAGCGCG‑tttttttta                                                                                                                             <  1:258931/140‑3 (MQ=255)          tttACCGAGGTTAATTTCGCTACTGGCTTGCGCACTCCACAAACGCAGCGTGTTGGTCGCGTCGGTGTCGTAACCAGGGATTATCTGATCGTAAGCGACTCCCAGAATCTCTTCGGTTTCAATCCAGCGCG‑tttttttta                                                                                                                             <  2:471055/140‑3 (MQ=255)            tACCGAGGTTAATTTCGCTACTGGCTTGCGCACTCCACAAACGCAGCGTGTTGGTCGCGTCGGTGTCGTAACCAGGGATTATCTGATCGTAAGCGACTCCCAGAATCTCTTCGGTTTCAATCCAGCGCG‑ttttttttacc                                                                                                                           <  1:202132/140‑5 (MQ=255)               cGAGGTTAATTTCGCTACTGGCTTGCGCACTCCACAAACGCAGCGTGTTGGTCGCGTCGGTGTCGTAACCAGGGATTATCTGATCGTAAGCGACTCCCAGAATCTCTTCGGTTTCAATCCAGCGCGTTTTTTTTACCTTc                                                                                                                         <  2:731243/140‑1 (MQ=255)                             cTACTGGCTTGCGCACTCCACAAACGCAGCGTGTTGGTCGCGTCGGTGTCGTAACCAGGGATTATCTGATCGTAAGCGACTCCCAGAATCTCTTCGGTTTCAATCCAGCGCGTTTTTTTTACCTTCCTGCTGAATGCGAc                                                                                                           >  1:54815/1‑140 (MQ=255)                                    cTTGCGCACTCCACAAACGCAGCGTGTTGGTCGCGTCGGTGTCGTAACCAGGGATTATCTGATCGTAAGCGACTCCCAGAATCTCTTCGGTTTCAATCCAGCGCGTTTTTTTTACCTTCCTGCTGAATGCGACCGCCaaa                                                                                                    <  1:295658/140‑1 (MQ=255)                                        cgcACTCCACAAACGCAGCGTGTTGGTCGCGTCGGTGTCGTAACCAGGGATTATCTGATCGTAAGCGACTCCCAGAATCTCTTCGGTTTCAATCCAGCGCGTTTTTTTTACCTTCCTGCTGAATGCGACCGCCAAAACgg                                                                                                >  2:332036/1‑140 (MQ=255)                                             tCCACAAACGCAGCGTGTTGGTCGCGTCGGTGTCGTAACCAGGGATTATCTGATCGTAAGCGACTCCCAGAATCTCTTCGGTTTCAATCCAGCGCGTTTTTTTTACCTTCCTGCTGAATGCGACCGCCAAAAc                                                                                                  >  1:292425/1‑133 (MQ=255)                                             tCCACAAACGCAGCGTGTTGGTCGCGTCGGTGTCGTAACCAGGGATTATCTGATCGTAAGCGACTCCCAGAATCTCTTCGGTTTCAATCCAGCGCGTTTTTTTTACCTTCCTGCTGAATGCGACCGCCAAAAc                                                                                                  <  2:292425/133‑1 (MQ=255)                                             tCCACAAACGCAGCGTGTTGGTCGCGTCGGTGTCGTAACCAGGGATTATCTGATCGTAAGCGACTCCCAGAATCTCTTCGGTTTCAATCCAGCGCGTTTTTTTTACCTTCCTGCTGAATGCGACCGCCAAAACGGACttt                                                                                           >  2:320176/1‑140 (MQ=255)                                                  aaaCGCAGCGTGTTGGTCGCGTCGGTGTCGTAACCAGGGATTATCTGATCGTAAGCGACTCCCAGAATCTCTTCGGTTTCAATCCAGCGCGTTTTTTTTACCTTCCTGCTGAATGCGACCGCCAAAACGGACTTTATAgc                                                                                      >  1:588527/1‑140 (MQ=255)                                                       cAGCGTGTTGGTCGCGTCGGTGTCGTAACCAGGGATTATCTGATCGTAAGCGACTCCCAGAATCTCTTCGGTTTCAATCCAGCGCGTTTTTTTTACCTTCCTGCTGAATGCGACCGCCAAAACGGACTTTATAGCGCgtg                                                                                 >  1:482193/1‑140 (MQ=255)                                                       cAGCGTGTTGGTCGCGTCGGTGTCGTAACCAGGGATTATCTGATCGTAAGCGACTCCCAGAATCTCTTCGGTTTCAATCCAGCGCGTTTTTTTTACCTTCCTGCTGAATGCGACCGCCAAAACGGACTTTATAGCGCgtg                                                                                 >  2:720529/1‑140 (MQ=255)                                                                      gTCGGTGTCGTAACCAGGGATTATCTGATCGTAAGCGACTCCCAGAATCTCTTCGGTTTCAATCCAGCGCGTTTTTTTTACCTTCCTGCTGAATGCGACCGCCAAAACGGACTTTATAGCGCGTGTTGTGGCGTTTGAAt                                                                  >  1:455134/1‑140 (MQ=255)                                                                         ggTGTCGTAACCAGGGATTATCTGATCGTAAGCGACTCCCAGAATCTCTTCGGTTTCAATCCAGCGCGTTTTTTTTACCTTCCTGCTGAATGCGACCGCCAAAACGGACTTTATAGCGCGTGTTGTGGCGTTTg                                                                     >  2:809245/1‑134 (MQ=255)                                                                         ggTGTCGTAACCAGGGATTATCTGATCGTAAGCGACTCCCAGAATCTCTTCGGTTTCAATCCAGCGCGTTTTTTTTACCTTCCTGCTGAATGCGACCGCCAAAACGGACTTTATAGCGCGTGTTGTGGCGTTTg                                                                     <  1:809245/134‑1 (MQ=255)                                                                         ggTGTCGTAACCAGGGATTATCTGATCGTAAGCGACTCCCAGAATCTCTTCGGTTTCAATCCAGCGCGTTTTTTTTACCTTCCTGCTGAATGCGACCGCCAAAACGGACTTTATAGCGCGTGTTGTGGCGTTTGAATTcc                                                               >  1:552581/1‑140 (MQ=255)                                                                            gtCGTAACCAGGGATTATCTGATCGTAAGCGACTCCCAGAATCTCTTCGGTTTCAATCCAGCGCGTTTTTTTTACCTTCCTGCTGAATGCGACCGCCAAAACGGACTTTATAGCGCGTGTTGTGGCGTTTGAATTCCCAc                                                            >  1:275937/1‑140 (MQ=255)                                                                               gTAACCAGGGATTATCTGATCGTAAGCGACTCCCAGAATCTCTTCGGTTTCAATCCAGCGCGTTTTTTTTCCCTTCCTGCTGAATGCGACCGCCAAAACGGACTTTATAGCGCGTGTTGTGGCGTTTGAATTCCCACggg                                                         <  1:199869/140‑1 (MQ=255)                                                                               gTAACCAGGGATTATCTGATCGTAAGCGACTCCCAGAATCTCTTCGGTTTCAATCCAGCGCGTTTTTTTTACCTTCCTGCTGAATGCGACCGCCAAAACGGACTTTATAGCGCGTGTTGTGGCGTTTGAATTCCCACggg                                                         <  2:552102/140‑1 (MQ=255)                                                                                tAACCAGGGATTATCTGATCGTAAGCGACTCCCAGAATCTCTTCGGTTTCAATCCAGCGCGTTTTTTTTACCTTCCTGCGAAATGCGACCGCCAAAACGGACTTTATAGCGCGTGTTGTGGCGTTTGAATTCCCACGGGt                                                        <  1:243390/140‑1 (MQ=255)                                                                                     aGGGATTATCTGATCGTAAGCGACTCCCAGAATCTCTTCGGTTTCAATCCAGCGCGTTTTTTTTACCTTCCTGCTGAATGCGACCGCCAAAACGGACTTTATAGCGCGTGTTGTGGCGTTTGAATTCCCACGGGTTACCg                                                   >  2:870714/1‑140 (MQ=255)                                                                                     aGGGATTATCTGATCGTAAGCGACTCCCAGAATCTCTTCGGTTTCAATCCAGCGCGTTTTTTTTACCTTCCTGCTGAATGCGACCGCCAAAACGGACTTTATAGCGCGTGTTGTGGCGTTTGAATTCCCACGGGTTACCg                                                   >  1:744469/1‑140 (MQ=255)                                                                                     aGGGATTATCTGATCGTAAGCGACTCCCAGAATCTCTTCGGTTTCAATCCAGCGCGTTTTTTTTACCTTCCTGCTGAATGCGACCGCCAAAACGGACTTTATAGCGCGTGTTGTGGCGTTTGAATTCCCACGGGTTACCg                                                   >  2:900498/1‑140 (MQ=255)                                                                                       ggATTATCTGATCGTAAGCGACTCCCAGAATCTCTTCGGTTTCAATCCAGCGCGTTTTTTTTACCTTCCTGCTGAATGCGACCGCCAAAACGGACTTTATAGCGCGTGTTGTGGCGTTTGAATTCCCACGGGTTACCGt                                                  <  1:259856/139‑1 (MQ=255)                                                                                       ggATTATCTGATCGTAAGCGACTCCCAGAATCTCTTCGGTTTCAATCCAGCGCGTTTTTTTTACCTTCCTGCTGAATGCGACCGCCAAAACGGACTTTATAGCGCGTGTTGTGGCGTTTGAATTCCCACGGGTTACCGt                                                  >  2:259856/1‑139 (MQ=255)                                                                                       ggATTATCTGATCGTAAGCGACTCCCAGAATCTCTTCGGTTTCAATCCAGCGCGTTTTTTTTACCTTCCTGCTGAATGCGACCGCCAAAACGGACTTTATAGCGCGTGTTGTGGCGTTTGAATTCCCACGGGTTACCGTa                                                 >  2:70969/1‑140 (MQ=255)                                                                                       ggATTATCTGATCGTAAGCGACTCCCAGAATCTCTTCGGTTTCAATCCAGCGCGTTTTTTTTACCTTCCTGCTGAATGCGACCGCCAAAACGGACTTTATAGCGCGTGTTGTGGCGTTTGAATTCCCACGGGTTACCGTa                                                 >  2:528215/1‑140 (MQ=255)                                                                                           tATCTGATCGTAAGCGACTCCCAGAATCTCTTCGGTTTCAATCCAGCGCGTTTTTTTTACCTTCCTGCTGAATGCGACCGCCAAAACGGACTTTATAGCGCGTGTTGTGGCGTTTGAATTCCCACGGGTTACCGTATTcc                                             <  2:275937/140‑1 (MQ=255)                                                                                            aTCTGATCGTAAGCGACTCCCAGAATCTCTTCGGTTTCAATCCAGCGCGTTTTTTTTACCTTCCTGCTGAATGCGACCGCCAAAACGGACTTTATAGCGCGTGTTGTGGCGTTTGAATTCCCACGGGTTACCGTATTcca                                            <  1:785083/140‑1 (MQ=255)                                                                                             tCTGATCGTAAGCGACTCCCAGAATCTCTTCGGTTTCAATCCAGCGCGTTTTTTTTACCTTCCTGCTGAATGCGACCGCCAAAACGGACTTTATAGCGCGTGTTGTGGCGTTTGAATTCCCACGGGTTACCGTATTccag                                           >  2:388148/1‑140 (MQ=255)                                                                                                 aTCGTAAGCGACTCCCAGAATCTCTTCGGTTTCAATCCAGCGCGTTTTTTTTACCTTCCTGCTGAATGCGACCGCCAAAACGGACTTTATAGCGCGTGTTGTGGCGTTTGAATTCCCACGGGTTACCGTATTccagccag                                       >  1:72391/1‑140 (MQ=255)                                                                                                     tAAGCGACTCCCAGAATCTCTTCGGTTTCAATCCAGCGCGTTTTTTTTACCTTCCTGCTGAATGCGACCACCAAAACGGACTTTATAGCGCGTGTTGTGGCGTTTGAATTCCCACGGGTTACCGTATTCCAGCCagtagt                                   >  2:898313/1‑140 (MQ=255)                                                                                                       aGCGACTCCCAGAATCTCTTCGGTTTCAATCCAGCGCGTTTTTTTTACCTTCCTGCTGAATGCGACCGCCAAAACGGACTTTATAGCGCGTGTTGTGGCGTTTGAATTCCCACGGGTTACCGTATTCCAGCCAGTAGTCt                                 >  1:778069/1‑140 (MQ=255)                                                                                                       aGCGACTCCCAGAATCTCTTCGGTTTCAATCCAGCGCGTTTTTTTTACCTTCCTGCTGAATGCGACCGCCAAAACGGACTTTATAGCGCGTGTTGTGGCGTTTGAATTCCCACGGGTTACCGTATTCCAGCCAGTAGTCt                                 >  1:483872/1‑140 (MQ=255)                                                                                                        gCGACTCCCAGAATCTTTTCGGTTTCAATCCAGCGCGTTTTTTTTACCTTCCTGCTGAATGCGACCGCCAAAACGGACTTTATAGCGCGTGTTGTGGCGTTTGAATTCCCACGGGTTACCGTATTCCAGCCAGTAGTCTg                                <  2:778069/140‑1 (MQ=255)                                                                                                        gCGACTCCCAGAATCTCTTCGGTTTCAATCCAGCGCGTTTTTTTTACCTTCCTGCTGAATGCGACCGCCAAAACGGACTTTATAGCGCGTGTTGTGGCGTTTGAATTCCCACGGGTTACCGTATTCCAGCCAGTAGTCTg                                <  2:483872/140‑1 (MQ=255)                                                                                                          gACTCCCAGAATCTCTTCGGTTTCAATCCAGCGCGTTTTTTTTACCTTCCTGCTGAATGCGACCGCCAAAACGGACTTTATAGCGCGTGTTGTGGCGTTTGAATTCCCACGGGTTACCGTATTCCAGCCAGTAGTCTGGc                              <  2:699408/140‑1 (MQ=255)                                                                                                           aCTCCCAGAATCTCTTCGGTTTCAATCCAGCGCGTTTTTTTTACCTTCCTGCTGAATGCGACCGCCAAAACGGACTTTATAGCGCGTGTTGTGGCGTTTGAATTCCCACGGGTTACCGTATTCCAGCCAGTAGTCTGGCg                             >  2:87510/1‑140 (MQ=255)                                                                                                            cTCCCAGAATCTCTTCGGTTTCAATCCAGCGCGTTTTTTTTACCTTCCTGCTGAATGCGACCGCCAAAACGGACTTTATAGCGCGTGTTGTGGCGTTTGAATTCCCACGGGTTACCGTATTCCAGCCAGTAGTCTGGCGa                            <  1:388148/140‑1 (MQ=255)                                                                                                            cTCCCAGAATCTCTTCGGTTTCAATCCAGCGCGTTTTTTTTACCTTCCTGCTGAATGCGACCGCCAAAACGGACTTTATAGCGCGTGTTGTGGCGTTTGAATTCCCACGGGTTACCGTATTCCAGCCAGTAGTCTGGCGa                            >  1:695532/1‑140 (MQ=255)                                                                                                             tCCCAGAATCTCTTCGGTTTCAATCCAGCGCGTTTTTTTTACCTTCCTGCTGAATGCGACCGCCAAAACGGACTTTATAGCGCGTGTTGTGGCGTTTGAATTCCCACGGGTTACCGTATTCCAGCCAGTAGTCTGGCGAc                           <  1:870714/140‑1 (MQ=255)                                                                                                             tCCCAGAATCTCTTCGGTTTCAATCCAGCGCGTTTTTTTTACCTTCCTGCTGAATGCGACCGCCAAAACGGACTTTATAGCGCGTGTTGTGGCGTTTGAATTCCCACGGGTTACCGTATTCCAGCCAGTAGTCTGGCGAc                           <  1:70969/140‑1 (MQ=255)                                                                                                             tCCCAGAATCTCTTCGGTTTCAATCCAGCGCGTTTTTTTTACCTTCCTGCTGAATGCGACCGCCAAAACGGACTTTATAGCGCGTGTTGTGGCGTTTGAATTCCCACGGGTTACCGTATTCCAGCCAGTAGTCTGGCGAc                           >  1:791233/1‑140 (MQ=255)                                                                                                               ccAGAATCTCTTCGGTTTCAATCCAGCGCGTTTTTTTTACCTTCCTGCTGAATGCGACCGCCAAAACGGACTTTATAGCGCGTGTTGTGGCGTTTGAATTCCCACGGGTTACCGTATTCCAGCCAGTAGTCTGGCGActc                         >  2:947937/1‑140 (MQ=255)                                                                                                                      ctctTCGGTTTCAATCCAGCGCGTTTTTTTTACCTTCCTGCTGAATGCGACCGCCAAAACGGACTTTATAGCGCGTGTTGTGGCGTTTGAATTCCCACGGGTTACCGTATTCCAGCCAGTAGTCTGGCGACTCTTTCTgg                  <  2:72391/140‑1 (MQ=255)                                                                                                                       tctTCGGTTTCAATCCAGCGCGTTTTTTTTACCTTCCTGCTGAATGCGACCGCCAAAACGGACTTTATAGCGCGTGTTGTGGCGTTTGAATTCCCACGGGTTACCGTATTCCAGCCAGTAGTCTGGCGACTCTTTCTGGc                 >  1:752222/1‑140 (MQ=255)                                                                                                                       tctTCGGTTTCAATCCAGCGCGTTTTTTTTACCTTCCTGCTGAATGCGACCGCCAAAACGGACTTTATAGCGCGTGTTGTGGCGTTTGAATTCCCACGGGTTACCGTATTCCAGCCAGTAGTCTGGCGACTCTTTCTGGc                 <  1:947937/140‑1 (MQ=255)                                                                                                                        ctTCGGTTTCAATCCAGCGCGTTTTTTTTACCTTCCTGCTGAATGCGACCGCCAAAACGGACTTTATAGCGCGTGTTGTGGCGTTTGAATTCCCACGGGTTACCGTATTCCAGCCAGTAGTCTGGCGACTCTTTCTGGCt                <  2:54815/140‑1 (MQ=255)                                                                                                                        ctTCGGTTTCAATCCAGCGCGTTTTTTTTACCTTCCTGCTGAATGCGACCGCCAAAACGGACTTTATAGCGCGTGTTGTGGCGTTTGAATTCCCACGGGTTACCGTATTCCAGCCAGTAGTCTGGCGACTCTTTCTGGCt                <  1:523039/140‑1 (MQ=255)                                                                                                                        ctTCGGTTTCAATCCAGCGCGTTTTTTTTACCTTCCTGCTGAATGCGACCGCCAAAACGGACTTTATAGCGCGTGTTGTGGCGTTTGAATTCCCACGGGTTACCGTATTCCAGCCAGTAGTCTGGCGACTCTTTCTGGCt                <  2:791233/140‑1 (MQ=255)                                                                                                                        ctTCGGTTTCAATCCAGCGCGTTTTTTTTACCTTCCTGCTGAATGCGACCGCCAAAACGGACTTTATAGCGCGTGTTGTGGCGTTTGAATTCCCACGGGTTACCGTATTCCAGCCAGTAGTCTGGCGACTCTTTCTGGCt                >  1:181511/1‑140 (MQ=255)                                                                                                                        ctTCGGTTTCAATCCAGCGCGTTTTTTTTACCTTCCTGCTGAATGCGACCGCCAAAACGGACTTTATAGCGCGTGTTGTGGCGTTTGAATTCCCACGGGTTACCGTATTCCAGCCAGTAGTCTGGCGACTCTTTCTGGCt                >  1:297748/1‑140 (MQ=255)                                                                                                                             gTTTCAATCCAGCGCGTTTTTTTTACCTTCCTGCTGAATGCGACCGCCAAAACGGACTTTATAGCGCGTGTTGTGGCGTTTGAATTCCCACGGGTTACCGTATTCCAGCCAGTAGTCTGGCGACTCTTTCTGGCTACCGt           <  1:720529/140‑1 (MQ=255)                                                                                                                             gTTTCAATCCAGCGCGTTTTTTTTACCTTCCTGCTGAATGCGACCGCCAAAACGGACTTTATAGCGCGTGTTGTGGCGTTTGAATTCCCACGGGTTACCGTATTCCAGCCAGTAGTCTGGCGACTCTTTCTGGCTACCGt           <  1:320176/140‑1 (MQ=255)                                                                                                                                      cAGCGCGTTTTTTTTACCTTCCCGTCGACGGCGTCCCGCAAAACGGACTTTATAGCGCGTGTTGTGGCGTTTGAATTCCCACGGGTTACCGTATTCCAGCCAGTAGTCTGGCGCCTCTTTCTGGCTACCGTTAACGATGt  <  2:679694/140‑1 (MQ=255)                                                                                                                                             |                                                                                                                                     CTGGTTGAATTTACCGAGGTTAATTTCGCTACTGGCTTGCGCACTCCACAAACGCAGCGTGTTGGTCGCGTCGGTGTCGTAACCAGGGATTATCTGATCGTAAGCGACTCCCAGAATCTCTTCGGTTTCAATCCAGCGCG‑TTTTTTTACCTTCCTGCTGAATGCGACCGCCAAAACGGACTTTATAGCGCGTGTTGTGGCGTTTGAATTCCCACGGGTTACCGTATTCCAGCCAGTAGTCTGGCGACTCTTTCTGGCTACCGTTAACGATGT  >  NC_000913/3565835‑3566106

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑