| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 923,255 | (G)5→6 | intergenic (+22/‑9) | clpS → / → clpA | specificity factor for ClpA‑ClpP chaperone‑protease complex/ATP‑dependent Clp protease ATP‑binding subunit ClpA |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 923,250 | 1 | . | G | 93.5% | 155.2 / 3.4 | 46 | intergenic (+17/‑14) | clpS/clpA | specificity factor for ClpA‑ClpP chaperone‑protease complex/ATP‑dependent Clp protease ATP‑binding subunit ClpA |
| Reads supporting (aligned to +/- strand): ref base . (2/1); new base G (22/21); total (24/22) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TACCAGGGGAAGGCCATTTGCGGAGTCTTTACCGCCGAGGTTGCAGAAACCAAAGTGGCGATGGTGAACAAGTACGCGAGGGAGAATGAGCATCCATTGCTGTGTACGCTAGAAAAAGCCTGAATGCAGGCATAAAAATT‑GG‑GGGAGGTGCCTATGCTCAATCAAGAACTGGAACTCAGTTTAAATATGGCTTTCGCCAGAGCGCGCGAGCACCGTCATGAGTTTATGACCGTCGAGCACTTGTTACTGGCGCTGCTCAGTAACCCAT > NC_000913/923111‑923378 | tACCAGGGGAAGGCCATTTGCGGAGTCTTTACCGCCGAGGTTGCAGAAACCAAAGTGGCGATGGTGAACAAGTACGCGAGGGAGAATGAGCATCCATTGCTGTGTACGCTAGAAAAAGCCTGAATGCAGGCATAAAAAtt > 2:549855/1‑140 (MQ=255) ggggAAGGCCATTTGCGGAGTCTTTACCGCCGAGGTTGCAGAAACCAAAGTGGCGATGGTGAACAAGTACGCGAGGGAGAATGAGCATCCATTGCTGTGTACGCTAGAAAAAGCCTGAATGCAGGCATAAAAATT‑gg‑ggg > 1:229855/1‑140 (MQ=255) ggAAGGCCATTTGCGGAGTCTTTACCGCCGAGGTTGCAGAAACCAAAGTGGCGATGGTGAACAAGTACGCGAGGGAGAATGAGCATCCATTGCTGTGTACGCTAGAAAAAGCCTGAATGCAGGCATAAAAATT‑gg‑gggga < 1:549855/140‑3 (MQ=255) gAAGGCCATTTGCGGAGTCTTTACCGCCGAGGTTGCAGAAACCAAAGTGGCGATGGTGAACAAGTACGCGAGGGAGAATGAGCATCCATTGCTGTGTACGCTAGAAAAAGCCTGAATGCAGGCATAAAAATT‑gg‑ggggag < 1:215331/140‑4 (MQ=255) gAAGGCCATTTGCGGAGTCTTTACCGCCGAGGTTGCAGAAACCAAAGTGGCGATGGTGAACAAGTACGCGAGGGAGAATGAGCATCCATTGCTGTGTACGCTAGAAAAAGCCTGAATGCAGGCATAAAAATT‑gg‑ggggag < 1:805184/140‑4 (MQ=255) aaGGCCATTTGCGGAGTCTTTACCGCCGAGGTTGCAGAAACCAAAGTGGCGATGGTGAACAAGTACGCGAGGGAGAATGAGCATCCATTGCTGTGTACGCTAGAAAAAGCCTGAATGCAGGCATAAAAATT‑gg‑ggggagg < 1:498889/140‑5 (MQ=255) aGGCCATTTGCGGAGTCTTTACCGCCGAGGTTGCAGAAACCAAAGTGGCGATGGTGAACAAGTACGCGAGGGAGAATGAGCATCCATTGCTGTGTACGCTAGAAAAAGCCTGAATGCAGGCATAAAAATT‑gg‑ggggaggt > 2:54039/1‑135 (MQ=255) gCCATTTGCGGAGTCTTTACCGCCGAGGTTGCAGAAACCAAAGTGGCGATGGTGAACAAGTACGCGAGGGAGAATGAGCATCCATTGCTGTGTACGCTAGAAAAAGCCTGAATGCAGGCATAAAAATTGGG‑GGGAGGTGc > 1:938152/1‑140 (MQ=255) gCCATTTGCGGAGTCTTTACCGCCGAGGTTGCAGAAACCAAAGTGGCGATGGTGAACAAGTACGCGAGGGAGAATGAGCATCCATTGCTGTGTACGCTAGAAAAAGCCTGAATGCAGGCATAAAAATTGGG‑GGGAGGTGc < 1:112551/140‑1 (MQ=255) gCCATTTGCGGAGTCTTTACCGCCGAGGTTGCAGAAACCAAAGTGGCGATGGTGAACAAGTACGCGAGGGAGAATGAGCATCCATTGCTGTGTACGCTAGAAAAAGCCTGAATGCAGGCATAAAAATTGGG‑GGGAGGTGc > 1:647568/1‑140 (MQ=255) gCCATTTGCGGAGTCTTTACCGCCGAGGTTGCAGAAACCAAAGTGGCGATGGTGAACAAGTACGCGAGGGAGAATGAGCATCCATTGCTGTGTACGCTAGAAAAAGCCTGAATGCAGGCATAAAAATTGGG‑GGGAGGTGc > 1:626033/1‑140 (MQ=255) gCCATTTGCGGAGTCTTTACCGCCGAGGTTGCAGAAACCAAAGTGGCGATGGTGAACAAGTACGCGAGGGAGAATGAGCATCCATTGCTGTGTACGCTAGAAAAAGCCTGAATGCAGGCATAAAAATTGGG‑GGGAGGTGc > 2:372666/1‑140 (MQ=255) tttGCGGAGTCTTTACCGCCGAGGTTGCAGAAACCAAAGTGGCGATGGTGAACAAGTACGCGAGGGAGAATGAGCATCCATTGCTGTGTACGCTAGAAAAAGCCTGAATGCAGGCATAAAAATTGGG‑GGGAGGTGCCTAt > 2:66257/1‑140 (MQ=255) ttGCGGAGTCTTTACCGCCGAGGTTGCAGAAACCAAAGTGGCGATGGTGAACAAGTACGCGAGGGAGAATGAGCATCCATTGCTGTGTACGCTAGAAAAAGCCTGAATGCAGGCATAAAAATTGGG‑GGGAGGTGCCTATg > 1:72380/1‑140 (MQ=255) gAGTCTTTACCGCCGAGGTTGCAGAAACCAAAGTGGCGATGGTGAACAAGTACGCGAGGGAGAATGAGCATCCATTGCTGTGTACGCTAGAAAAAGCCTGAATGCAGGCATAAAAATTGGG‑GGGAGGTGCCTATGCtcaa > 1:777841/1‑140 (MQ=255) tCTTTACCGCCGAGGTTGCAGAAACCAAAGTGGCGATGGTGAACAAGTACGCGAGGGAGAATGAGCATCCATTGCTGTGTACGCTAGAAAAAGCCTGAATGCAGGCATAAAAATTGGG‑GGGAGGTGCCTATGCtcaatca < 1:54039/140‑1 (MQ=255) tttACCGCCGAGGTTGCAGAAACCAAAGTGGCGATGGTGAACAAGTACGCGAGGGAGAATGAGCATCCATTGCTGTGTACGCTAGAAAAAGCCTGAATGCAGGCATAAAAATTGGG‑GGGAGGTGCCTATGCTCAATCAAg < 2:751600/140‑1 (MQ=255) cgAGGTTGCAGAAACCAAAGTGGCGATGGTGAACAAGTACGCGAGGGAGAATGAGCATCCATTGCTGTATACGCTAGAAAAAGCCTGAATGCAGGCATAAAAATTGGG‑GGGAGGTGCCTATGCTCAATCAAGAACTGGaa < 2:764281/140‑1 (MQ=255) cgAGGTTGCAGAAACCAAAGTGGCGATGGTGAACAAGTACGCGAGGGAGAATGAGCATCCATTGCTGTATACGCTAGAAAAAGCCTGAATGCAGGCATAAAAATTGGG‑GGGAGGTGCCTATGCTCAATCAAGAACTGGaa > 1:764281/1‑140 (MQ=255) aGGTTGCAGAAACCAAAGTGGCGATGGTGAACAAGTACGCGAGGGAGAATGAGCATCCATTGCTGTGTACGCTAGAAAAAGCCTGAATGCAGGCATAAAAATTGGG‑GGGAGGTGCCTATGCTCAATCAAGAACTGGAACt < 1:370835/140‑1 (MQ=255) aGGTTGCAGAAACCAAAGTGGCGATGGTGAACAAGTACGCGAGGGAGAATGAGCATCCATTGCTGTGTACGCTAGAAAAAGCCTGAATGCAGGCATAAAAATTGGG‑GGGAGGTGCCTATGCTCAATCAAGAACTGGAACt < 2:229855/140‑1 (MQ=255) aGGTTGCAGAAACCAAAGTGGCGATGGTGAACAAGTACGCGAGGGAGAATGAGCATCCATTGCTGTGTACGCTAGAAAAAGCCTGAATGCAGGCATAAAAATTGGG‑GGGAGGTGCCTATGCTCAATCAAGAACTGGAACt < 2:938152/140‑1 (MQ=255) tGCAGAAACCAAAGTGGCGATGGTGAACAAGTACGCGAGGGAGAATGAGCATCCATTGCTGTGTACGCTAGAAAAAGCCTGAATGCAGGCATAAAAATTGGG‑GGGAGGTGCCTATGCTCAATCAAGAACTGGAACTCAGt < 2:777841/140‑1 (MQ=255) aCCAAAGTGGCGATGGTGAACAAGTACGCGAGGGAGAATGAGCATCCATTGCTGTGTACGCTAGAAAAAGCCTGAATGCAGGCATAAAAATTGGG‑GGGAGGTGCCTATGCTCAATCAAGAACTGGAACTCAGTTTAAata < 2:647568/140‑1 (MQ=255) aaGTGGCGATGGTGAACAAGTACGCGAGGGAGAATGAGCATCCATTGCTGTGTACGCTAGAAAAAGCCTGAATGCAGGCATAAAAATTGGG‑GGGAGGTGCCTATGCTCAATCAAGAACTGGAACTCAGTTTAAATATGGc < 2:626033/140‑1 (MQ=255) tACGCGAGGGAGAATGAGCATCCATTGCTGTGTACGCTAGAAAAAGCCTGAATGCAGGCATAAAAATTGGG‑GGGAGGTGCCTATGCTCAATCAAGAACTGGAACTCAGTTTAAATATGGCTTTCGCCAGAGCGCGCGAGc < 2:837538/140‑1 (MQ=255) aCGCGAGGGAGAATGAGCATCCATTGCTGTGTACGCTAGAAAAAGCCTGAATGCAGGCATAAAAATTGGG‑GGGAGGTGCCTATGCTCAATCAAGAACTGGAACTCAGTTTAAATATGGCTTTCGCCAGAGCGCGCGAGCa < 1:66257/140‑1 (MQ=255) aCGCGAGGGAGAATGAGCATCCATTGCTGTGTACGCTAGAAAAAGCCTGAATGCAGGCATAAAAATTGGG‑GGGAGGTGCCTATGCTCAATCAAGAACTGGAACTCAGTTTAAATATGGCTTTCGCCAGAGCGCGCGAGCa > 2:393643/1‑140 (MQ=255) cgAGGGAGAATGAGCATCCATTGCTGTGTACGCTAGAAAAAGCCTGAATGCAGGCATAAAAATTGGG‑GGGAGGTGCCTATGCTCAATCAAGAACTGGAACTCAGTTTAAATATGGCTTTCGCCAGAGCGCGCGAGCACCg > 1:406054/1‑140 (MQ=255) cgAGGGAGAATGAGCATCCATTGCTGTGTACGCTAGAAAAAGCCTGAATGCAGGCATAAAAATT‑GG‑GGGAGGTGCCTATGCTCAATCAAGAACTGGAACTCAGTTTAAATATGGCTTTCGCCagag < 1:831159/126‑1 (MQ=255) cgAGGGAGAATGAGCATCCATTGCTGTGTACGCTAGAAAAAGCCTGAATGCAGGCATAAAAATT‑GG‑GGGAGGTGCCTATGCTCAATCAAGAACTGGAACTCAGTTTAAATATGGCTTTCGCCagag > 2:831159/1‑126 (MQ=255) gggAGAATGAGCATCCATTGCTGTGTACGCTAGAAAAAGCCTGAATGCAGGCATAAAAATT‑GGTGGGAGGTGCCTATGCTCAATCAAGAACTGGAACTCAGTTTAAATATGGCTTTCGCCAGAGCGCGCGAGCACCGTCa > 2:615533/1‑140 (MQ=255) gCATCCATTGCTGTGTACGCTAGAAAAAGCCTGAATGCAGGCATAAAAATTGGG‑GGGAGGTGCCTATGCTCAATCAAGAACTGGAACTCAGTTTAAATATGGCTTTCGCCAGAGCGCGCGAGCACCGTCATGAGTTTATg > 2:845544/1‑140 (MQ=255) cATCCATTGCTGTGTACGCTAGAAAAAGCCTGAATGCAGGCATAAAAATTGGG‑GGGAGGTGCCTATGCTCAATCAAGAACTGGAACTCAGTTTAAATATGGCTTTCGCCAGAGCGCGCGAGCACCGTCATGAGTTTATGa > 2:609280/1‑140 (MQ=255) cATCCATTGCTGTGTACGCTAGAAAAAGCCTGAATGCAGGCATAAAAATTGGG‑GGGAGGTGCCTATGCTCAATCAAGAACTGGAACTCAGTTTAAATATGGCTTTCGCCAGAGCGCGCGAGCACCGTCATGAGTTTATGa > 1:66733/1‑140 (MQ=255) gtgtACGCTAGAAAAAGCCTGAATGCAGGCATAAAAATTGGG‑GGGAGGTGCCTATGCTCAATCAAGAACTGGAACTCAGTTTAAATATGGCTTTCGCCAGAGCGCGCGAGCACCGTCATGAGTTTATGACCGTCGAGCAc > 2:951680/1‑140 (MQ=255) gtACGCTAGAAAAAGCCTGAATGCAGGCATAAAAATTGGG‑GGGAGGTGCCTATGCTCAATCAAGAACTGGAACTCAGTTTAAATATGGCTTTCGCCAGAGCGCGCGAGCACCGTCATGAGTTTATGACCGTCGAGCACtt < 1:372666/140‑1 (MQ=255) gtACGCTAGAAAAAGCCTGAATGCAGGCATAAAAATTGGG‑GGGAGGTGCCTATGCTCAATCAAGAACTGGAACTCAGTTTAAATATGGCTTTCGCCAGAGCGCGCGAGCACCGTCATGAGTTTATGACCGTCGAGCACtt > 2:830446/1‑140 (MQ=255) tACGCTAGAAAAAGCCTGAATGCAGGCATAAAAATTGGG‑GGGAGGTGCCTATGCTCAATCAAGAACTGGAACTCAGTTTAAATATGGCTTTCGCCAGAGCGCGCGAGCACCGTCATGAGTTTATGACCGTCGAGCACTTg > 1:559818/1‑140 (MQ=255) cGCTAGAAAAAGCCTGAATGCAGGCATAAAAATTGGG‑GGGAGGTGCCTATGCTCAATCAAGAACTGGAACTCAGTTTAAATATGGCTTTCGCCAGAGCGCGCGAGCACCGTCATGAGTTTATGACCGTCGAGCACTTGtt > 1:321345/1‑140 (MQ=255) gCTAGAAAAAGCCTGAATGCAGGCATAAAAATTGGG‑GGGAGGTGCCTATGCTCAATCAAGAACTGGAACTCAGTTTAAATATGGCTTTCGCCAGAGCGCGCGAGCACCGTCATGAGTTTATGACCg > 1:8083/1‑126 (MQ=255) gCTAGAAAAAGCCTGAATGCAGGCATAAAAATTGGG‑GGGAGGTGCCTATGCTCAATCAAGAACTGGAACTCAGTTTAAATATGGCTTTCGCCAGAGCGCGCGAGCACCGTCATGAGTTTATGACCg < 2:8083/126‑1 (MQ=255) cTAGAAAAAGCCTGAATGCAGGCATAAAAATTGGG‑GGGAGGTGCCTATGCTCAATCAAGAACTGGAACTCAGTTTAAATATGGCTTTCGCCAGAGCGCGCGAGCACCGTCATGAGTTTATGACCGTCGAGCACTTGTTAc < 1:830446/140‑1 (MQ=255) aaaaGCCTGAATGCAGGCATAAAAATTGGG‑GGGAGGTGCCTATGCTCAATCAAGAACTGGAACTCAGTTTAAATATGGCTTTCGCCAGAGCGCGCGAGCACCGTCATGAGTTTATGACCGTCGAGCACTTGTTACTGgcg < 2:559818/140‑1 (MQ=255) aGCCTGAATGCAGGCATAAAAATTGGG‑GGGAGGTGCCTATGCTCAATCAAGAACTGGAACTCAGTTTAAATATGGCTTTCGCCAGAGCGCGCGAGCACCGTCATGAGTTTATGACCGTCGAGCACTTGTTACTGGCgctg < 2:66733/140‑1 (MQ=255) aGCCTGAATGCAGGCATAAAAATTGGG‑GGGAGGTGCCTATGCTCAATCAAGAACTGGAACTCAGTTTAAATATGGCTTTCGCCAGAGCGCGCGAGCACCGTCATGAGTTTATGACCGTCGAGCACTTGTTACTGGCgctg > 2:209662/1‑140 (MQ=255) gAATGCAGGCATAAAAATTGGG‑GGGAGGTGCCTATGCTCAATCAAGAACTGGAACTCAGTTTAAATATGGCTTTCGCCAGAGCGCGCGAGCACCGTCATGAGTTTATGACCGTCGAGCACTTGTTACTGGCGCTGCTCAg > 1:353061/1‑140 (MQ=255) tGCAGGCATAAAAATTGGG‑GGGAGGTGCCTATGCTCAATCAAGAACTGGAACTCAGTTTAAATATGGCTTTCGCCAGAGCGCGCGAGCACCGTCATGAGTTTATGACCGTCGAGCACTTGTTACTGGCGCTGCTCAGTaa < 1:609280/140‑1 (MQ=255) ggCATAAAAATTGGG‑GGGAGGTGCCTATGCTCAATCAAGAACTGGAACTCAGTTTAAATATGGCTTTCGCCAGAGCGCGCGAGCACCGTCATGAGTTTATGACCGTCGAGCACTTGTTACTGgcg > 1:854086/1‑125 (MQ=255) ggCATAAAAATTGGG‑GGGAGGTGCCTATGCTCAATCAAGAACTGGAACTCAGTTTAAATATGGCTTTCGCCAGAGCGCGCGAGCACCGTCATGAGTTTATGACCGTCGAGCACTTGTTACTGgcg < 2:854086/125‑1 (MQ=255) gCATAAAAATTGGG‑GGGAGGTGCCTATGCTCAATCAAGAACTGGAACTCAGTTTAAATATGGCTTTCGCCAGAGCGCGCGAGCACCGTCATGAGTTTATGACCGTCGAGCACTTGTTACTGGCGCTGCTCAGTGACCCAt < 2:353061/140‑1 (MQ=255) gCATAAAAATTGGG‑GGGAGGTGCCTATGCTCAATCAAGAACTGGAACTCAGTTTAAATATGGCTTTCGCCAGAGCGCGCGAGCACCGTCATGAGTTTATGACCGTCGAGCACTTGTTACTGGCGCTGCTCAGTAACCCAt < 1:468463/140‑1 (MQ=255) gCATAAAAATTGGG‑GGGAGGTGCCTATGCTCAATCAAGAACTGGAACTCAGTTTAAATATGGCTTTCGCCAGAGCGCGCGAGCACCGTCATGAGTTTATGACCGTCGAGCACTTGTTACTGGCGCTGCTCAGTAACCCAt > 1:383851/1‑140 (MQ=255) | TACCAGGGGAAGGCCATTTGCGGAGTCTTTACCGCCGAGGTTGCAGAAACCAAAGTGGCGATGGTGAACAAGTACGCGAGGGAGAATGAGCATCCATTGCTGTGTACGCTAGAAAAAGCCTGAATGCAGGCATAAAAATT‑GG‑GGGAGGTGCCTATGCTCAATCAAGAACTGGAACTCAGTTTAAATATGGCTTTCGCCAGAGCGCGCGAGCACCGTCATGAGTTTATGACCGTCGAGCACTTGTTACTGGCGCTGCTCAGTAACCCAT > NC_000913/923111‑923378 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |