Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,727,494	(G)6→7	coding (836/1098 nt)	nemA →	N‑ethylmaleimide reductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,727,488	1	.	G	100.0%	150.1 / NA	42	A277G (GCG→GGG)	nemA	N‑ethylmaleimide reductase
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base G (22/20);  total (22/20)

TCGCGTTTCACCAATCGGTACTTTCCAGAACACAGATAACGGCCCGAATGAAGAAGCCGATGCACTGTATCTGATTGAACAACTGGGTAAACGCGGCATTGCTTATCTGCATATGTCAGAACCAGATTGGGC‑GGGGGGTGAACCGTATACTGATGCGTTCCGCGAAAAAGTACGCGCCCGTTTCCACGGTCCGATTATCGGCGCAGGTGCATACACAGTAGAAAAAGCTGAAACGCTGATCGGCAAAGGGTTAATTGATGCGGTG  >  NC_000913/1727357‑1727621                                                                                                                                     |                                                                                                                                      tCGCGTTTCACCAATCGGTACTTTCCAGAACACAGATAACGGCCCGAATGAAGAAGCCGATGCACTGTATCTGATTGAACAACTGGGTAAACGCGGCATTGCTTATCTGCATATGTCAGAACCAGATTGGGC‑gggggggt                                                                                                                               <  1:663368/140‑3 (MQ=255)    cgTTTCACCAATCGGTACTTTCCAGAACACAGATAACGGCCCGAATGAAGAAGCCGATGCACTGTATCTGATTGAACAACTGGGTAAACGCGGCATTGCTTATCTGCATATGTCAGAACCAGATTGGGC‑gggggggtgaa                                                                                                                            <  2:648884/140‑6 (MQ=255)    cgTTTCACCAATCGGTACTTTCCAGAACACAGATAACGGCCCGAATGAAGAAGCCGATGCACTGTATCTGATTGAACAACTGGGTAAACGCGGCATTGCTTATCTGCATATGTCAGAACCAGATTGGGC‑gggggggtgaa                                                                                                                            <  2:307694/140‑6 (MQ=255)         cACCAATCGGTACTTTCCAGAACACAGATAACGGCCCGAATGAAGAAGCCGATGCACTGTATCTGATTGAACAACTGGGTAAACGCGGCATTGCTTATCTGCATATGTCAGAACCAGATTGGGCGGGGGGGTGAACCGta                                                                                                                        <  1:928689/140‑1 (MQ=255)         cACCAATCGGTACTTTCCAGAACACAGATAACGGCCCGAATGAAGAAGCCGATGCACTGTATCTGATTGAACAACTGGGTAAACGCGGCATTGCTTATCTGCATATGTCAGAACCAGATTGGGCGGGGGGGTGAACCGta                                                                                                                        <  1:271358/140‑1 (MQ=255)                 ggTACTTTCCAGAACACAGATAACGGCCCGAATGAAGAAGCCGATGCACTGTATCTGATTGAACAACTGGGTAAACGCGGCATTGCTTATCTGCATATGTCAGAACCAGATTGGGCGGGGGGGTGAACCGTATACTGATg                                                                                                                >  1:708513/1‑140 (MQ=255)                           aGAACACAGATAACGGCCCGAATGAAGAAGCCGATGCACTGTATCTGATTGAACAACTGGGTAAACGCGGCATTGCTTATCTGCATATGTCAGAACCAGATTGGGCGGGGGGGTGAACCGTATACTGATGCGTTCCGCGa                                                                                                      >  2:135839/1‑140 (MQ=255)                             aaCACAGATAACGGCCCGAATGAAGAAGCCGATGCACTGTATCTGATTGAACAACTGGGTAAACGCGGCATTGCTTATCTGCATATGTCAGAACCAGATTGGGCGGGGGGGTGAACCGTATACTGATGCGTTCCGCGaaa                                                                                                    >  2:534801/1‑140 (MQ=255)                                acaGATAACGGCCCGAATGAAGAAGCCGATGCACTGTATCTGATTGAACAACTGGGTAAACGCGGCATTGCTTATCTGCATATGTCAGAACCAGATTGGGCGGGGGGGTGAACCGTATACTGATGCGTTCCGCGAAAAAg                                                                                                 >  1:622094/1‑140 (MQ=255)                                  aGATAACGGCCCGAATGAAGAAGCCGATGCACTGTATCTGATTGAACAACTGGGTAAACGCGGCATTGCTTATCTGCATATGTCAGAACCAGATTGGGCGGGGGGGTGAACCGTATACTGATGCGTTCCGCGAAAAAGTa                                                                                               >  1:374609/1‑140 (MQ=255)                                  aGATAACGGCCCGAATGAAGAAGCCGATGCACTGTATCTGATTGAACAACTGGGTAAACGCGGCATTGCTTATCTGCATATGTCAGAACCAGATTGGGCGGGGGGGTGAACCGTATACTGATGCGTTCCGCGAAAAAGTa                                                                                               >  1:402716/1‑140 (MQ=255)                                  aGATAACGGCCCGAATGAAGAAGCCGATGCACTGTATCTGATTGAACAACTGGGTAAACGCGGCATTGCTTATCTGCATATGTCAGAACCAGATTGGGCGGGGGGGTGAACCGTATACTGATGCGTTCCGCGAAAAAGTa                                                                                               >  2:211561/1‑140 (MQ=255)                                  aGATAACGGCCCGAATGAAGAAGCCGATGCACTGTATCTGATTGAACAACTGGGTAAACGCGGCATTGCTTATCTGCATATGTCAGAACCAGATTGGGCGGGGGGGTGAACCGTATACTGATGCGTTCCGCGAAAAAGTa                                                                                               >  1:680601/1‑140 (MQ=255)                                      aaCGGCCCGAATGAAGAAGCCGATGCACTGTATCTGATTGAACAACTGGGTAAACGCGGCATTGCTTATCTGCATATGTCAGAACCAGATTGGGCGGGGGGGTGAACCGTATACTGATGCGTTCCGCGAAAAAGTAcgcg                                                                                           <  1:648249/140‑1 (MQ=255)                                       aCGGCCCGAATGAAGAAGCCGATGCACTGTATCTGATTGAACAACTGGGTAAACGCGGCATTGCTTATCTGCATATGTCAGAACCAGATTGGGCGGGGGGGTGAACCGTATACTGATGCGTTCCGCGAAAAAGTACgcgc                                                                                          >  1:946111/1‑140 (MQ=255)                                        cGGCCCGAATGAAGAAGCCGATGCACTGTATCTGATTGAACAACTGGGTAAACGCGGCATTGCTTATCTGCATATGTCAGAACCAGATTGGGCGGGGGGGTGAACCGTATACTGATGCGTTCCGCGAAAAAGTACGCGcc                                                                                         <  1:534801/140‑1 (MQ=255)                                              gAATGAAGAAGCCGATGCACTGTATCTGATTGAACAACTGGGTAAACGCGGCATTGCTTATCTGCATATGTCAGAACCAGATTGGGCGGGGGGGTGAACCGTATACTGATGCGTTCCGCGAAAAAGTACGCGCCCGTTTc                                                                                   >  2:795696/1‑140 (MQ=255)                                               aaTGAAGAAGCCGATGCACTGTATCTGATTGAACAACTGGGTAAACGCGGCATTGCTTATCTGCATATGTCAGAACCAGATTGGGCGGGGGGGTGAACCGTATACTGATGCGTTCCGCGAAAAAGTACGCGCCCGTTTcc                                                                                  <  2:946111/140‑1 (MQ=255)                                                aTGAAGAAGCCGATGCACTGTATCTGATTGAACAACTGGGTAAACGCGGCATTGCTTATCTGCATATGTCAGAACCAGATTGGGCGGGGGGGTGAACCGTATACTGATGCGTTCCGCGAAAAAGTACGCGCCCGTTTCCa                                                                                 <  1:795696/140‑1 (MQ=255)                                                            aTGCACTGTATCTGATTGAACAACTGGGTAAACGCGGCATTGCTTATCTGCATATGTCAGAACCAGATTGGGCGGGGGGGTGAACCGTATACTGATGCGTTCCGCGAAAAAGTACGCGCCCGTTTCCACGGTCCGATTAt                                                                     <  2:402716/140‑1 (MQ=255)                                                             tGCACTGTATCTGATTGAACAACTGGGTAAACGCGGCATTGCTTATCTGCATATGTCAGAACCAGATTGGGCGGGGGGGTGAACCGTATACTGATGCGTTCCGCGAAAAAGTACGCGCCCGTTTCCa                                                                                 >  1:781434/1‑127 (MQ=255)                                                             tGCACTGTATCTGATTGAACAACTGGGTAAACGCGGCATTGCTTATCTGCATATGTCAGAACCAGATTGGGCGGGGGGGTGAACCGTATACTGATGCGTTCCGCGAAAAAGTACGCGCCCGTTTCCa                                                                                 <  2:781434/127‑1 (MQ=255)                                                                   gTATCTGATTGAACAACTGGGTAAACGCGGCATTGCTTATCTGCATATGTCAGAACCAGATTGGGCGGGGGGGTGAACCGTATACTGATGCGTTCCGCGAAAAAGTACGCGCCCGTTTCCACGGTCCGATTATCGGCGCa                                                              <  1:211561/140‑1 (MQ=255)                                                                           ttGAACAACTGGGTAAACGCGGCATTGCTTATCTGCATATGTCAGAACCAGATTGGGCGGGGGGGTGAACCGTATACTGATGCGTTCCGCGAAAAAGTACGCGCCCGTTTCCACGGTCCGATTATCGGCGCAGGTGCATa                                                      <  2:374609/140‑1 (MQ=255)                                                                              aacaacTGGGTAAACGCGGCATTGCTTATCTGCATATGTCAGAACCAGATTGGGCGGGGGGGTGAACCGTATACTGATGCGTTCCGCGAAAAAGTACGCGCCCGTTTCCACGGTCCGATTATCGgcg                                                                >  2:291162/1‑127 (MQ=255)                                                                              aacaacTGGGTAAACGCGGCATTGCTTATCTGCATATGTCAGAACCAGATTGGGCGGGGGGGTGAACCGTATACTGATGCGTTCCGCGAAAAAGTACGCGCCCGTTTCCACGGTCCGATTATCGgcg                                                                <  1:291162/127‑1 (MQ=255)                                                                              aacaacTGGGTAAACGCGGCATTGCTTATCTGCATATGTCAGAACCAGATTGGGCGGGGGGGTGAACCGTATACTGATGCGTTCCGCGAAAAAGTACGCGCCCGTTTCCACGGTCCGATTATCGGCGCAGGTGCATacac                                                   >  2:99110/1‑140 (MQ=255)                                                                              aacaacTGGGTAAACGCGGCATTGCTTATCTGCATATGTCAGAACCAGATTGGGCGGGGGGGTGAACCGTATACTGATGCGTTCCGCGAAAAAGTACGCGCCCGTTTCCACGGTCCGATTATCGGCGCAGGTGCATacac                                                   <  2:968/140‑1 (MQ=255)                                                                              aacaacTGGGTAAACGCGGCATTGCTTATCTGCATATGTCAGAACCAGATTGGGCGGGGGGGTGAACCGTATACTGATGCGTTCCGCGAAAAAGTACGCGCCCGTTTCCACGGTCCGATTATCGGCGCAGGTGCATacac                                                   >  1:968/1‑140 (MQ=255)                                                                              aacaacTGGGTAAACGCGGCATTGCTTATCTGCATATGTCAGAACCAGATTGGGCGGGGGGGTGAACCGTATACTGATGCGTTCCGCGAAAAAGTACGCGCCCGTTTCCACGGTCCGATTATCGGCGCAGGTGCATa                                                      <  1:525892/137‑1 (MQ=255)                                                                              aacaacTGGGTAAACGCGGCATTGCTTATCTGCATATGTCAGAACCAGATTGGGCGGGGGGGTGAACCGTATACTGATGCGTTCCGCGAAAAAGTACGCGCCCGTTTCCACGGTCCGATTATCGGCGCAGGTGCATa                                                      >  2:525892/1‑137 (MQ=255)                                                                                          aaCGCGGCATTGCTTATCTGCATATGTCAGAACCAGATTGGGCGGGGGGGTGAACCGTATACTGATGCGTTCCGCGAAAAAGTACGCGCCCATTTCCACGGTCCGATTATCGGCGCAGGTGCATACACAGTAGAAAAAGc                                       >  2:886890/1‑140 (MQ=255)                                                                                                gCATTGCTTATCTGCATATGTCAGAACCAGATTGGGCGGGGGGGTGAACCGTATACTGATGCGTTCCGCGAAAAAGTACGCGCCCGTTTCCACGGTCCGATTATCGGCGCAGGTGCATACACAGTAGAAAAAGCTGAAAc                                 >  2:21273/1‑140 (MQ=255)                                                                                                gCATTGCTTATCTGCATATGTCAGAACCAGATTGGGCGGGGGGGTGAACCGTATACTGATGCGTTCCGCGAAAAAGTACGCGCCCGTTTCCACGGTCCGATTATCGGCGCAGGTGCATACACAGTAGAAAAAGCTGAAAc                                 >  2:164399/1‑140 (MQ=255)                                                                                                  aTTGCTTATCTGCATATGTCAGAACCAGATTGGGCGGGGGGGTGAACCGTATACTGATGCGTTCCGCGAAAAAGTACGCGCCCGTTTCCACGGTCCGATTATCGGCGCAGGTGCATACACAGTAGAAAAAGCTGAAACGc                               >  1:590912/1‑140 (MQ=255)                                                                                                          tCTGCATATGTCAGAACCAGATTGGGCGGGGGGGTGAACCGTATACTGATGCGTTCCGCGAAAAAGTACGCGCCCGTTTCCACGGTCCGATTATCGGCGCAGGTGCATACACAGTAGAAAAAGCTGAAACGCTGATCGGc                       <  1:124491/140‑1 (MQ=255)                                                                                                            tGCATATGTCAGAACCAGATTGGGCGGGGGGGTGAACCGTATACTGATGCGTTCCGCGAAAAAGTACGCGCCCGTTTCCACGGTCCGATTATCGGCGCAGGTGCATACACAGTAGAAAAAGCTGAAACGCTGATCGGCaa                     <  1:99110/140‑1 (MQ=255)                                                                                                            tGCATATGTCAGAACCAGATTGGGCGGGGGGGTGAACCGTATACTGATGCGTTCCGCGAAAAAGTACGCGCCCGTTTCCACGGTCCGATTATCGGCGCAGGTGCATACACAGTAGAAAAAGCTGAAACGCTGATCGGCaa                     <  1:21273/140‑1 (MQ=255)                                                                                                              cATATGTCAGAACCAGATTGGGCGGGGGGGTGAACCGTATACTGATGCGTTCCGCGAAAAAGTACGCGCCCGTTTCCACGGTCCGATTATCGGCGCAGGTGCATACACAGTAGAAAAAGCTGAAACGCTGa                            >  2:516969/1‑131 (MQ=255)                                                                                                              cATATGTCAGAACCAGATTGGGCGGGGGGGTGAACCGTATACTGATGCGTTCCGCGAAAAAGTACGCGCCCGTTTCCACGGTCCGATTATCGGCGCAGGTGCATACACAGTAGAAAAAGCTGAAACGCTGa                            <  1:516969/131‑1 (MQ=255)                                                                                                                tatGTCAGAACCAGATTGGGCGGGGGGGTGAACCGTATACTGATGCGTTCCGCGAAAAAGTACGCGCCCGTTTCCACGGTCCGATTATCGGCGCAGGTGCATACACAGTAGAAAAAGCTGAAACGCTGATCGGCAAAggg                 >  1:207476/1‑140 (MQ=255)                                                                                                                     cAGAACCAGATTGGGCGGGGGGGTGAACCGTATACTGATGCGTTCCGCGAAAAAGTACGCGCCCGTTTCCACGGTCCGATTATCGGCGCAGGTGCATACACAGTAGAAAAAGCTGAAACGCTGATCGGCAAAGGGTTAAt            <  2:708513/140‑1 (MQ=255)                                                                                                                        aaCCAGATTGGGCGGGGGGGTGAACCGTATACTGATGCGTTCCGCGAAAAAGTACGCGCCCGTTTCCACGGTCCGATTATCGGCGCAGGTGCATACACAGTAGAAAAAGCTGAAACGCTGATCGGCAAAGGGTTAATTGa         >  2:718645/1‑140 (MQ=255)                                                                                                                             gATTGGGCGGGGGGGTGAACCGTATACTGATGCGTTCCGCGAAAAAGTACGCGCCCGTTTCCACGGTCCGATTATCGGCGCAGGTGCATACACAGTAGAAAAAGCTGAAACGCTGATCGGCAAAGGGTTAATTGATGCgg    >  2:21420/1‑140 (MQ=255)                                                                                                                             gATTGGGCGGGGGGGTGAACCGTATACTGATGCGTTCCGCGAAAAAGTACGCGCCCATTTCCACGGTCCGATTATCGGCGCAGGTGCATACACAGTAGAAAAAGCTGAAACGCTGATCGGCAAAGGGTTAATTGATGCgg    <  1:886890/140‑1 (MQ=255)                                                                                                                               ttGGGCGGGGGGGTGAACCGTATACTGATGCGTTCCGCGAAAAAGTACGCGCCCGTTTCCACGGTCCGATTATCGGCGCAGGTGCATACACAGTAGAAAAAGCTGAAACGCTGATCGGCAAAGGGTTAATTGATGCGGTg  <  2:590912/140‑1 (MQ=255)                                                                                                                                     |                                                                                                                                      TCGCGTTTCACCAATCGGTACTTTCCAGAACACAGATAACGGCCCGAATGAAGAAGCCGATGCACTGTATCTGATTGAACAACTGGGTAAACGCGGCATTGCTTATCTGCATATGTCAGAACCAGATTGGGC‑GGGGGGTGAACCGTATACTGATGCGTTCCGCGAAAAAGTACGCGCCCGTTTCCACGGTCCGATTATCGGCGCAGGTGCATACACAGTAGAAAAAGCTGAAACGCTGATCGGCAAAGGGTTAATTGATGCGGTG  >  NC_000913/1727357‑1727621

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑