Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,790,055	(C)5→6	coding (1190/1437 nt)	ydiU ←	UPF0061 family protein YdiU

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,790,050	1	.	C	100.0%	136.7 / NA	38	R399G (CGT→GGT)	ydiU	UPF0061 family protein YdiU
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base C (16/22);  total (16/22)

GGTGCAATTCCGTCATATCACCCTTTTCTGCCGCTTCAATCGCCCGTTGCGCCAACCAGTTGCGCAATACCAGAGCGGGGTTAACGCTTTGCATCAGTTGCTGACGCTCACTATCGCTAACCTCGTCTTGTTGCAAACG‑CCCCCGATAACGGGCAAACCAGTCATCAAATGCCGCACGATCAATAAACTCATCACGTAGCGGTGACGCCGCGCTGTGCTGCTCGGTCAGACTCAGCATGCGGAATGTGCGGGTATAATCGCTGCGCTCTCG  >  NC_000913/1789912‑1790182                                                                                                                                            |                                                                                                                                     ggTGCAATTCCGTCATATCACCCTTTTCTGCCGCTTCAATCGCCCGTTGCGCCAACCAGTTGCGCAATACCAGAGCGGGGTTAACGCTTTGCATCAGTTGCTGACGCTCACTATCGCTAACCTCGTCTTGTTGCAAACG‑c                                                                                                                                     >  1:850259/1‑140 (MQ=255) ggTGCAATTCCGTCATATCACCCTTTTCTGCCGCTTCAATCGCCCGTTGCGCCAACCAGTTGCGCAATACCAGAGCGGGGTTAACGCTTTGCATCAGTTGCTGACGCTCACTATCGCTAACCTCGTCTTGTTGCAAACG‑c                                                                                                                                     >  1:818566/1‑140 (MQ=255)  gTGCAATTCCGTCATATCACCCTTTTCTTCCGCTTCAATCGCCCGTTGCGCCAACCAGTTGCGCAATACCAGAGCGGGGTTAACGCTTTGCATCAGTTGCTGACGCTCACTATCGCTAACCTCGTCTTGTTGCAAACG‑cc                                                                                                                                    >  1:583169/1‑140 (MQ=255)   tGCAATTCCGTCATATCACCCTTTTCTGCCGCTTCAATCGCCCGTTGCGCCAACCAGTTGCGCAATACCAGAGCGGGGTTAACGCTTTGCATCAGTTGCTGACGCTCACTATCGCTAACCTCGTCTTGTTGCAAACG‑ccc                                                                                                                                   >  2:334368/1‑140 (MQ=255)            gTCATATCACCCTTTTCTGCCGCTTCAATCGCCCGTTGCGCCAACCAGTTGCGCAATACCAGAGCGGGGTTAACGCTTTGCATCAGTTGCTGACGCTCACTATCGCTAACCTCGTCTTGTTGCAAACGCCCCCCGATAAc                                                                                                                           <  1:610012/140‑1 (MQ=255)                  tCACCCTTTTCTGCCGCTTCAATCGCCCGTTGCGCCAACCAGTTGCGCAATACCAGAGCGGGGTTAACGCTTTGCATCAGTTGCTGACGCTCACTATCGCTAACCTCGTCTTGTTGCAAACGCCCCCCGATAACGGGCaa                                                                                                                     >  1:341796/1‑140 (MQ=255)                     cccTTTTCTGCCGCTTCAATCGCCCGTTGCGCCAACCAGTTGCGCAATACCAGAGCGGGGTTAACGCTTTGCATCAGTTGCTGACGCTCACTATCGCTAACCTCGTCTTGTTGCAAACGCCCCCCGATAACGGGCAAAcc                                                                                                                  >  2:759091/1‑140 (MQ=255)                           tCTGCCGCTTCAATCGCCCGTTGCGCCAACCAGTTGCGCAATACCAGAGCGGGGTTAACGCTTTGCATCAGTTGCTGACGCTCACTATCGCTAACCTCGTCTTGTTGCAAACGCCCCCCGATAACGGGCAAACCAGtcat                                                                                                            <  2:818566/140‑1 (MQ=255)                                       aTCGCCCGTTGCGCCAACCAGTTGCGCAATACCAGAGCGGGGTTAACGCTTTGCATCAGTTGCTGACGCTCACTATCGCTAACCTCGTCTTGTTGCAAACGCCCCCCGATAACGGGCAAACCAGTCATCAAATGCCGCAc                                                                                                <  2:850259/140‑1 (MQ=255)                                       aTCGCCCGTTGCGCCAACCAGTTGCGCAATACCAGAGCGGGGTTAACGCTTTGCATCAGTTGCTGACGCTCACTATCGCTAACCTCGTCTTGTTGCAAACGCCCCCCGATAACGGGCAAACCAGTCATCAAATGCCGCAc                                                                                                <  1:518795/140‑1 (MQ=255)                                       aTCGCCCGTTGCGCCAACCAGTTGCGCAATACCAGAGCGGGGTTAACGCTTTGCATCAGTTGCTGACGCTCACTATCGCTAACCTCGTCTTGTTGCAAACGCCCCCCGATAACGGGCAAACCAGTCATCAAATGCCGCAc                                                                                                <  1:295446/140‑1 (MQ=255)                                        tCGCCCGTTGCGCCAACCAGTTGCGCAATACCAGAGCGGGGTTAACGCTTTGCATCAGTTGCTGACGCTCACTATCGCTAACCTCGTCTTGTTGCAAACGCCCCCCGATAACGGGCAAACCAGTCATCAAATGCCGCACg                                                                                               <  1:279595/140‑1 (MQ=255)                                         cGCCCGTTGCGCCAACCAGTTGCGCAATACCAGAGCGGGGTTAACGCTTTGCATCAGTTGCTGACGCTCACTATCGCTAACCTCGTCTTGTTGCAAACGCCCCCCGATAACGGGCAAACCAGTCATCAAATGCCGCACGa                                                                                              <  1:352217/140‑1 (MQ=255)                                             cGTTGCGCCAACCAGTTGCGCAATACCAGAGCGGGGTTAACGCTTTGCATCAGTTGCTGACGCTCACTATCGCTAACCTCGTCTTGTTGCAAACGCCCCCCGATAACGGGCAAACCAGTCATCAAATGCCGCACGATCaa                                                                                          >  1:761468/1‑140 (MQ=255)                                              gTTGCGCCAACCAGTTGCGCAATACCAGAGCGGGGTTAACGCTTTGCATCAGTTGCTGACGCTCACTATCGCTAACCTCGTCTTGTTGCAAACGCCCCCCGATAACGGGCAAACCAGTCATCAAATGCCGCACGATCAAt                                                                                         <  1:334368/140‑1 (MQ=255)                                                      aaCCAGTTGCGCAATACCAGAGCGGGGTTAACGCTTTGCATCAGTTGCTGACGCTCACTATCGCTAACCTCGTCTTGTTGCAAACGCCCCCCGATAACGGGCAAACCAGTCATCAAATGCCGCACGATCAATAAACtcat                                                                                 <  1:759091/140‑1 (MQ=255)                                                                gcAATACCAGAGCGGGGTTAACGCTTTGCATCAGTTGCTGACGCTCACTATCGCTAACCTCGTCTTGTTGCAAACGCCCCCCGATAACGGGCAAACCAGTCATCAAATGCCGCACGATCAATAAACTCATCACGTAGCgg                                                                       <  2:583169/140‑1 (MQ=255)                                                                   aTACCAGAGCGGGGTTAACGCTTTGCATCAGTTGCTGACGCTCACTATCGCTAACCTCGTCTTGTTGCAAACGCCCCCCGATAACGGGCAAACCAGTCATCAAATGCCGCACGATCAATAAACTCATCACGTAGCGGTGa                                                                    >  2:370266/1‑140 (MQ=255)                                                                   aTACCAGAGCGGGGTTAACGCTTTGCATCAGTTGCTGACGCTCACTATCGCTAACCTCGTCTTGTTGCAAACGCCCCCCGATAACGGGCAAACCAGTCATCAAATGCCGCACGATCAATAAACTCATCACGTAGCGGTGa                                                                    >  2:751855/1‑140 (MQ=255)                                                                    tACCAGAGCGGTGTTAACGCTTTGCATCAGTTGCTGACGCTCACTATCGCTAACCTCGTCTTGTTGCAAACGCCCCCCGATAACGGGCAAACCAGTCATCAAATGCCGCACGATCAATAAACTCATCACGTAGCGGTGAc                                                                   >  1:714680/1‑140 (MQ=255)                                                                     aCCAGAGCGGGGTTAACGCTTTGCATCAGTTGCTGACGCTCACTATCGCTAACCTCGTCTTGTTGCAAACGCCCCCCGATAACGGGCAAACCAGTCATCAAATGCCGCACGATCAATAAACTCATCACGTAGCGGTGAcg                                                                  <  1:239560/140‑1 (MQ=255)                                                                     aCCAGAGCGGGGTTAACGCTTTGCATCAGTTGCTGACGCTCACTATCGCTAACCTCGTCTTGTTGCAAACGCCCCCCGATAACGGGCAAACCAGTCATCAAATGCCGCACGATCAATAAACTCATCACGTAGCGGTGAcg                                                                  <  2:761468/140‑1 (MQ=255)                                                                      ccAGAGCGGGGTTAACGCTTTGCATCAGTTGCTGACGCTCACTATCGCTAACCTCGTCTTGTTGCAAACGCCCCCCGATAACGGGCAAACCAGTCATCAAATGCCGCACGATCAATAAACTCATCACGTAGCGGTGAcgc                                                                 >  2:441764/1‑140 (MQ=255)                                                                      ccAGAGCGGGGTTAACGCTTTGCATCAGTTGCTGACGCTCACTATCGCTAACCTCGTCTTGTTGCAAACGCCCCCCGATAACGGGCAAACCAGTCATCAAATGCCGCACGATCAATAAACTCATCACGTAGCGGTGAcgc                                                                 >  1:954873/1‑140 (MQ=255)                                                                              gggTTAACGCTTTGCATCAGTTGCTGACGCTCACTATCGCTAACCTCGTCTTGTTGCAAACGCCCCCCGATAACGGGCAAACCAGTCATCAAATGCCGCACGATCAATAAACTCATCACGTAGCGGTGACGCCGCGCtgt                                                         <  1:441764/140‑1 (MQ=255)                                                                                gTTAACGCTTTGCATCAGTTGCTGACGCTCACTATCGCTAACCTCGTCTTGTTGCAAACGCCCCCCGATAACGGGCAAACCAGTCATCAAATGCCGCACGATCAATAAACTCATCACGTAGCGGTGACGCCGCGCTGtgc                                                       <  1:457109/140‑1 (MQ=255)                                                                                        tttGCATCAGTTGCTGACGCTCACTATCGCTAACCTCGTCTTGTTGCAAACGCCCCCCGATAACGGGCAAACCAGTCATCAAATGCCGCACGATCAATAAACTCATCACGTAGCGGTGACGCCGCGCTGTGCTGCTCGGt                                               <  2:341796/140‑1 (MQ=255)                                                                                         ttGCATCAGTTGCTGACGCTCACTATCGCTAACCTCGTCTTGTTGCAAACGCCCCCCGATAACGGGCAAACCAGTCATCAAATGCCGCACGATCAATAAACTCATCACGTAGCGGTGACGCCGCGCTGTGCTGCTCGGTc                                              >  2:426741/1‑140 (MQ=255)                                                                                                 gTTGCTGACGCTCACTATCGCTAACCTCGTCTTGTTGCAAACGCCCCCCGATAACGGGCAAACCAGTCATCAAATGCCGCACGATCAATAAACTCATCACGTAGCGGTGACGCCGCGCTGTGCTGCTCGGTCAGACTCAg                                      >  2:762572/1‑140 (MQ=255)                                                                                                    gCTGACGCTCACTATCGCTAACCTCGTCTTGTTGCAAACGCCCCCCGATAACGGGCAAACCAGTCATCAAATGCCGCACGATCAATAAACTCATCACGTAGCGGTGACGCCGCGCTGTGCTGCTCGGTCAGACTCAGCAt                                   >  1:317543/1‑140 (MQ=255)                                                                                                         cGCTCACTATCGCTAACCTCGTCTTGTTGCAAACGCCCCCCGATAACGGGCAAACCAGTCATCAAATGCCGCACGATCAATAAACTCATCACGTAGCGGTGACGCCGCGCTGTGCTGCTCGGTCAGACTCAGCATGCGGa                              <  1:426741/140‑1 (MQ=255)                                                                                                            tCACTATCGCTAACCTCGTCTTGTTGCAAACGCCCCCCGATAACGGGCAAACCAGTCATCAAATGCCGCACGATCAATAAACTCATCACGTAGCGGTGACGCCGCGCTGTGCTGCTCGGTCAGACTCAGCATGCGGAAtg                           >  2:627629/1‑140 (MQ=255)                                                                                                              aCTATCGCTAACCTCGTCTTGTTGCAAACGCCCCCCGATAACGGGCAAACCAGTCATCAAATGCCGCACGATCAATAAACTCATCACGTAGCGGTGACGCCGCGCTGTGCTGCTCGGTCAGACTCAGCATGCGGAAtgtg                         <  2:272565/140‑1 (MQ=255)                                                                                                              aCTATCGCTAACCTCGTCTTGTTGCAAACGCCCCCCGATAACGGGCAAACCAGTCATCAAATGCCGCACGATCAATAAACTCATCACGTAGCGGTGACGCCGCGCTGTGCTGCTCGGTCAGACTCAGCATGCGGAAtgtg                         <  2:192838/140‑1 (MQ=255)                                                                                                              aCTATCGCTAACCTCGTCTTGTTGCAAACGCCCCCCGATAACGGGCAAACCAGTCATCAAATGCCGCACGATCAATAAACTCATCACGTAGCGGTGACGCCGCGCTGTGCTGCTCGGTCAGACTCAGCATGCGGAAtgtg                         <  1:751855/140‑1 (MQ=255)                                                                                                                        aCCTCGTCTTGTTGCAAACGCCCCCCGATAACGGGCAAACCAGTCATCAAATGCCGCACGATCAATAAACTCATCACGTAGCGGTGACGCCGCGCTGTGCTGCTCGGTCAGACTCAGCATGCGGAATGTGCGGGTATAAt               >  2:31851/1‑140 (MQ=255)                                                                                                                            cGTCTTGTTGCAAACGCCCCCCGATAACGGGCAAACCAGTCATCAAATGCCGCACGATCAATAAACTCATCACGTAGCGGTGACGCCGCGCTGTGCTGCTCGGTCAGACTCAGCATGCGGAATGTGCGGGTATAATCGCt           >  1:609734/1‑140 (MQ=255)                                                                                                                              tCTTGTTGCAAACGCCCCCCGATAACGGGCAAACCAGTCATCAAATGCCGCACGATCAATAAACTCATCACGTAGCGGTGACGCCGCGCTGTGCTGCTCGGTCAGACTCAGCATGCGGAATGTGCGGGTATAATCGCTgc         >  1:836721/1‑140 (MQ=255)                                                                                                                              tCTTGTTGCAAACGCCCCCCGATAACGGGCAAACCAGTCATCAAATGCCGCACGATCAATAAACTCATCACGTAGCGGTGACGCCGCGCTGTGCTGCTCGGTCAGACTCAGCATGCGGAATGTGCGGGTATAATCGCTgc         >  2:84526/1‑140 (MQ=255)                                                                                                                                 tgttgCAAACGCCCCCCGATAACGGGCAAACCAGTCATCAAATGCCGCACGATCAATAAACTCATCACGTAGCGGTGACGCCGCGCTGTGCTGCTCGGTCAGACTCAGCATGCGGAATGTGCGGGTATAATCGCTGCGct      <  1:103088/140‑1 (MQ=255)                                                                                                                                     gCAAACGCCCCCCGATAACGGGCAAACCAGTCATCAAATGCCGCACGATCAATAAACTCATCACGTAGCGGTGACGCCGCGCTGTGCTGCTCGGTCAGACTCAGCATGCGGAATGTGCGGGTATAATCGCTGCGCTCTcg  <  1:762572/140‑1 (MQ=255)                                                                                                                                     gCAAACGCCCCCCGATAACGGGCAAACCAGTCATCAAATGCCGCACGATCAATAAACTCATCACGTAGCGGTGACGCCGCGCTGTGCTGCTCGGTCAGACTCAGCATGCGGAATGTGCGGGTATAATCGCTGCGCTCTcg  <  1:652779/140‑1 (MQ=255)                                                                                                                                            |                                                                                                                                     GGTGCAATTCCGTCATATCACCCTTTTCTGCCGCTTCAATCGCCCGTTGCGCCAACCAGTTGCGCAATACCAGAGCGGGGTTAACGCTTTGCATCAGTTGCTGACGCTCACTATCGCTAACCTCGTCTTGTTGCAAACG‑CCCCCGATAACGGGCAAACCAGTCATCAAATGCCGCACGATCAATAAACTCATCACGTAGCGGTGACGCCGCGCTGTGCTGCTCGGTCAGACTCAGCATGCGGAATGTGCGGGTATAATCGCTGCGCTCTCG  >  NC_000913/1789912‑1790182

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑