| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 2,518,016 | (C)5→6 | intergenic (‑184/+25) | pdeA ← / ← alaX | putative c‑di‑GMP phosphodiesterase PdeA/tRNA‑Ala |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,518,011 | 1 | . | C | 98.0% | 179.7 / ‑3.3 | 51 | intergenic (‑179/+30) | pdeA/alaX | putative c‑di‑GMP phosphodiesterase PdeA/tRNA‑Ala |
| Reads supporting (aligned to +/- strand): ref base . (0/1); new base C (21/29); total (21/30) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
AATAAGTTTATTCGCATCGCGAAGCAAACAGGGGGCCAGCCTGTTGCCGATTTTTCATCCAATGATGCTTTCACCAGGCGGCACTAACCCGGACGTAGACTGTATTACAAAAGCGGCAAAAAGCAGAGACAAAAA‑A‑CCCCCGCTTTGCAGCGAGGGTTGGAAATTTGGTGGAGCTAAGCGGGATCGAACCGCTGACCTCTTGCATGCCATGCAAGCGCTCTCCCAGCTGAGCTATAGCCCCACGATGCGTTTACGTACCAAGTTTGCT > NC_000913/2517876‑2518143 | aataaGTTTATTCGCATCGCGAAGCAAACAGGGGGCCAGCCTGTTGCCGATTTTTCATCCAATGATGCTTTCACCAGGCGGCACTAACCCGGACGTAGACTGTATTACAAAAGCGGCAAAAAGCAGAGACAAAAA‑A‑cccc > 1:460873/1‑140 (MQ=255) aGTTTATTCGCATCGCGAAGCAAACAGGGGGCCAGCCTGTTGCCGATTTTTCATCCAATGATGCTTTCACCAGGCGGCACTAACCCGGACGTAGACTGTATTACAAAAGCGGCAAAAAGCAGAGACAAAAA‑A‑ccccccgc > 1:683020/1‑137 (MQ=255) tATTCGCATCGCGAAGCAAACAGGGGGCCAGCCTGTTGCCGATTTTTCATCCAATGATGCTTTCACCAGGCGGCACTAACCCGGACGTAGACTGTATTACAAAAGCGGCAAAAAGCAGAGACAAAAA‑ACCCCCCGCTTTg > 2:846624/1‑140 (MQ=255) ttCGCATCGCGAAGCAAACAGGGGGCCAGCCTGTTGCCGATTTTTCATCCAATGATGCTTTCACCAGGCGGCACTAACCCGGACGTAGACTGTATTACAAAAGCGGCAAAAAGCAGAGACAAAAA‑ACCCCCCGCTTTGCa > 2:573519/1‑140 (MQ=255) gCATCGCGAAGCAAACAGGGGGCCAGCCTGTTGCCGATTTTTCATCCAATGATGCTTTCACCAGGCGGCACTAACCCGGACGTAGACTGTATTACAAAAGCGGCAAAAAGCAGAGACAAAAA‑ACCCCCCGCTTTGCAGCg > 1:555166/1‑140 (MQ=255) gCATCGCGAAGCAAACAGGGGGCCAGCCTGTTGCCGATTTTTCATCCAATGATGCTTTCACCAGGCGGCACTAACCCGGACGTAGACTGTATTACAAAAGCGGCAAAAAGCAGAGACAAAAA‑ACCCCCCGCTTTGCAGCg < 2:555166/140‑1 (MQ=255) cATCGCGAAGCAAACAGGGGGCCAGCCTGTTGCCGATTTTTCATCCAATGATGCTTTCACCAGGCGGCACTAACCCGGACGTAGACTGTATTACAAAAGCGGCAAAAAGCAGAGACAAAAA‑ACCCCCCGCTTTGCAGCGa < 1:290869/140‑1 (MQ=255) tCGCGAAGCAAACAGGGGGCCAGCCTGTTGCCGATTTTTCATCCAATGATGCTTTCACCAGGCGGCACTAACCCGGACGTAGACTGTATTACAAAAGCGGCAAAAAGCAGAGACAAAAA‑ACCCCCCGCTTTGCAGCGAgg > 1:57793/1‑140 (MQ=255) gcgAAGCAAACAGGGGGCCAGCCTGTTGCCGATTTTTCATCCAATGATGCTTTCACCAGGCGGCACTAACCCGGACGTAGACTGTATTACAAAAGCGGCAAAAAGCAGAGACAAAAA‑ACCCCCCGCTTTGCAGCGAGGGt < 1:573519/140‑1 (MQ=255) cgAAGCAAACAGGGGGCCAGCCTGTTGCCGATTTTTCATCCAATGATGCTTTCACCAGGCGGCACTAACCCGGACGTAGACTGTATTACAAAAGCGGCAAAAAGCAGAGACAAAAA‑ACCCCCCGCTTTGCAGCGAGGGtt < 2:720349/140‑1 (MQ=255) gCAAACAGGGGGCCAGCCTGTTGCCGATTTTTCATCCAATGATGCTTTCACCAGGCGGCACTAACCCGGACGTAGACTGTATTACAAAAGCGGCAAAAAGCAGAGACAAAAA‑ACCCCCCGCTTTGCAGCGAGGGTTGGaa > 2:930316/1‑140 (MQ=255) gCCAGCCTGTTGCCGATTTTTCATCCAATGATGCTTTCACCAGGCGGCACTAACCCGGACGTAGACTGTATTACAAAAGCGGCAAAAAGCAGAGACAAAAA‑ACCCCCCGCTTTGCAGCGAGGGTTGGAAATTTGGTGGAg < 1:538621/140‑1 (MQ=255) gCCAGCCTGTTGCCGATTTTTCATCCAATGATGCTTTCACCAGGCGGCACTAACCCGGACGTAGACTGTATTACAAAAGCGGCAAAAAGCAGAGACAAAAA‑ACCCCCCGCTTTGCAGCGAGGGTTGGAAATTTGGTGGAg > 2:538621/1‑140 (MQ=255) gCCAGCCTGTTGCCGATTTTTCATCCAATGATGCTTTCACCAGGCGGCACTAACCCGGACGTAGACTGTATTACAAAAGCGGCAAAAAGCAGAGACAAAAA‑ACCCCCCGCTTTGCAGCGAGGGTTGGAAATTTGGTGGAg > 2:19586/1‑140 (MQ=255) ccTGTTGCCGATTTTTCATCCAATGATGCTTTCACCAGGCGGCACTAACCCGGACGTAGACTGTATTACAAAAGCGGCAAAAAGCAGAGACAAAAA‑ACCCCCCGCTTTGCAGCGAGGGTTGGAAATTTGGTGGAGCTAAg < 2:889247/140‑1 (MQ=255) cGATTTTTCATCCAATGATGCTTTCACCAGGCGGCACTAACCCGGACGTAGACTGTATTACAAAAGCGGCAAAAAGCAGAGACAAAAA‑ACCCCCCGCTTTGCAGCGAGGGTTGGAAATTTGGTGGAGCTAAGCGGGATCg < 1:158420/140‑1 (MQ=255) gATTTTTCATCCAATGATGCTTTCACCAGGCGGCACTAACCCGGACGTAGACTGTATTACAAAAGCGGCAAAAAGCAGAGACAAAAA‑ACCCCCCGCTTTGCAGCGAGGGTTGGAAATTTGGTGGAGCTAAGCGGGATCGa < 1:238489/140‑1 (MQ=255) aTTTTTCATCCAATGATGCTTTCACCAGGCGGCACTAACCCGGACGTAGACTGTATTACAAAAGCGGCAAAAAGCAGAGACAAAAA‑ACCCCCCGCTTTGCAGCGAGGGTTGGAAATTTGGTGGAGCTAAGCGGGATCGaa > 2:627347/1‑140 (MQ=255) tttCATCCAATGATGCTTTCACCAGGCGGCACTAACCCGGACGTAGACTGTATTACAAAAGCGGCAAAAAGCAGAGACAAAAA‑ACCCCCCGCTTTGCAGCGAGGGTTGGAAATTTGGTGGAGCTAAGCGGGATCGAACCg < 1:930316/140‑1 (MQ=255) tttCATCCAATGATGCTTTCACCAGGCGGCACTAACCCGGACGTAGACTGTATTACAAAAGCGGCAAAAAGCAGAGACAAAAA‑ACCCCCCGCTTTGCAGCGAGGGTTGGAAATTTGGTGGAGCTAAGCGGGATCGAACCg < 1:627347/140‑1 (MQ=255) tCATCCAATGATGCTTTCACCAGGCGGCACTAACCCGGACGTAGACTGTATTACAAAAGCGGCAAAAAGCAGAGACAAAAA‑ACCCCCCGCTTTGCAGCGAGGGTTGGAAATTTGGTGGAGCTAAGCGGGATCGAACCGCt < 2:813117/140‑1 (MQ=255) tCATCCAATGATGCTTTCACCAGGCGGCACTAACCCGGACGTAGACTGTATTACAAAAGCGGCAAAAAGCAGAGACAAAAA‑ACCCCCCGCTTTGCAGCGAGGGTTGGAAATTTGGTGGAGCTAAGCGGGATCGAACCGCt < 2:57793/140‑1 (MQ=255) cATCCAATGATGCTTTCACCAGGCGGCACTAACCCGGACGTAGACTGTATTACAAAAGCGGCAAAAAGCAGAGACAAAAA‑ACCCCCCGCTTTGCAGCGAGGGTTGGAAATTTGGTGGAGCTAAGCGGGATCGAACCGCTg < 1:383764/140‑1 (MQ=255) cATCCAATGATGCTTTCACCAGGCGGCACTAACCCGGACGTAGACTGTATTACAAAAGCGGCAAAAAGCAGAGACAAAAA‑ACCCCCCGCTTTGCAGCGAGGGTTGGAAATTTGGTGGAGCTAAGCGGGATCGAACCGCTg < 1:503353/140‑1 (MQ=255) atgatgCTTTCACCAGGCGGCACTAACCCGGACGTAGACTGTATTACAAAAGCGGCAAAAAGCAGAGACAAAAA‑ACCCCCCGCTTTGCAGCGAGGGTTGGAAATTTGGTGGAGCTAAGCGGGATCGAACCGCt > 1:896104/1‑133 (MQ=255) atgatgCTTTCACCAGGCGGCACTAACCCGGACGTAGACTGTATTACAAAAGCGGCAAAAAGCAGAGACAAAAA‑ACCCCCCGCTTTGCAGCGAGGGTTGGAAATTTGGTGGAGCTAAGCGGGATCGAACCGCt < 2:896104/133‑1 (MQ=255) tgatgCTTTCACCAGGCGGCACTAACCCGGACGTAGACTGTATTACAAAAGCGGCAAAAAGCAGAGACAAAAA‑ACCCCCCGCTTTGCAGCGAGGGTTGGAAATTTGGTGGAGCTAAGCGGGATCGAACCGCTGACCTCtt > 2:501852/1‑140 (MQ=255) tgCTTTCACCAGGCGGCACTAACCCGGACGTAGACTGTATTACAAAAGCGGCAAAAAGCAGAGACAAAAA‑ACCCCCCGCTTTGCAGCGAGGGTTGGAAATTTGGTGGAGCTAAGCGGGATCGAACCGCTGACCTCTTGca < 1:845242/140‑1 (MQ=255) cccGGACGTAGACTGTATTACAAAAGCGGCAAAAAGCAGAGACAAAAA‑ACCCCCCGCTTTGCAGCGAGGGTTGGAAATTTGGTGGAGCTAAGCGGGATCGAACCGCTGACCTCTTGCATGCCATGCAAGCGCTCTCCCAg < 2:158529/140‑1 (MQ=255) cccGGACGTAGACTGTATTACAAAAGCGGCAAAAAGCAGAGACAAAAA‑ACCCCCCGCTTTGCAGCGAGGGTTGGAAATTTGGTGGAGCTAAGCGGGATCGAACCGCTGACCTCTTGCATGCCATGCAAGCGCTCTCCCAg > 1:158529/1‑140 (MQ=255) ccGGACGTAGACTGTATTACAAAAGCGGCAAAAAGCAGAGACAAAAA‑ACCCCCCGCTTTGCAGCGAGGGTTGGAAATTTGGTGGAGCTAAGCGGGATCGAACCGCTGACCTCTTGCATGCCATGCAAGCGCTCTCCCAGc < 1:501852/140‑1 (MQ=255) cGGACGTAGACTGTATTACAAAAGCGGCAAAAAGCAGAGACAAAAAGC‑CCCCCGCTTTGCAGCGAGGGTTGGAATTTTGGTGGAGCTAAGCGGGATCGAACCGCTGACCTCTTGCATGCCATGCAAGCGCTCTCCCAGCt < 1:846624/140‑1 (MQ=255) cGGACGTAGACTGTATTACAAAAGCGGCAAAAAGCAGAGACAAAAA‑ACCCCCCGCTTTGCAGCGAGGGTTGGAAATTTGGTGGAGCTAAGCGGGATCGAACCGCTGACCTCTTGCATGCCATGCAAGTGCTCTCCCAGCt < 1:698930/140‑1 (MQ=255) cGGACGTAGACTGTATTACAAAAGCGGCAAAAAGCAGAGACAAAAA‑ACCCCCCGCTTTGCAGCGAGGGTTGGAAATTTGGTGGAGCTAAGCGGGATCGAACCGCTGACCTCTTGCATGCCATGCAAGCGCTCTCCCAGCt < 1:19586/140‑1 (MQ=255) gACGTAGACTGTATTACAAAAGCGGCAAAAAGCAGAGACAAAAA‑ACCCCCCGCTTTGCAGCGAGGGTTGGAAATTTGGTGGAGCTAAGCGGGATCGAACCGCTGACCTCTTGCATGCCATGCAAGCGCTCTCCCAGCTGa > 1:932358/1‑140 (MQ=255) gACTGTATTACAAAAGCGGCAAAAAGCAGAGACAAAAA‑ACCCCCCGCTTTGCAGCGAGGGTTGGAAATTTGGTGGAGCTAAGCGGGATCGAACCGCTGACCTCTTGCATGCCATGCAAGCGCTCTCCCAg > 2:290517/1‑130 (MQ=255) gACTGTATTACAAAAGCGGCAAAAAGCAGAGACAAAAA‑ACCCCCCGCTTTGCAGCGAGGGTTGGAAATTTGGTGGAGCTAAGCGGGATCGAACCGCTGACCTCTTGCATGCCATGCAAGCGCTCTCCCAg < 1:290517/130‑1 (MQ=255) aCTGTATTACAAAAGCGGCAAAAAGCAGAGACAAAAA‑ACCCCCCGCTTTGCAGCGAGGGTTGGAAATTTGGTGGAGCTAAGCGGGATCGAACCGCTGACCTCTTGCATGCCATGCAAGCGCTCTCCCAGCTGAGCTATAg > 2:865477/1‑140 (MQ=255) tATTACAAAAGCGGCAAAAAGCAGAGACAAAAA‑ACCCCCCGCTTTGCAGCGAGGGTTGGAAATTTGGTGGAGCTAAGCGGGATCGAACCGCTGACCTCTTGCATGCCATGCAAGCGCTCTCCCAGCTGAGCta < 2:743821/133‑1 (MQ=255) tATTACAAAAGCGGCAAAAAGCAGAGACAAAAA‑ACCCCCCGCTTTGCAGCGAGGGTTGGAAATTTGGTGGAGCTAAGCGGGATCGAACCGCTGACCTCTTGCATGCCATGCAAGCGCTCTCCCAGCTGAGCta > 1:743821/1‑133 (MQ=255) tATTACAAAAGCGGCAAAAAGCAGAGACAAAAA‑ACCCCCCGCTTTGCAGCGAGGGTTGGAAATTTGGTGGAGCTAAGCGGGATCGAACCGCTGACCTCTTGCATGCCATGCAAGCGCTCTCCCAGCTGAGCTATAGcccc > 1:92377/1‑140 (MQ=255) aTTACAAAAGCGGCAAAAAGCAGAGACAAAAA‑ACCCCCCGCTTTGCAGCGAGGGTTGGAAATTTGGTGGAGCTAAGCGGGATCGAACCGCTGACCTCTTGCATGCCATGCAAGCGCTCTCCCAGc < 2:793584/125‑1 (MQ=255) aTTACAAAAGCGGCAAAAAGCAGAGACAAAAA‑ACCCCCCGCTTTGCAGCGAGGGTTGGAAATTTGGTGGAGCTAAGCGGGATCGAACCGCTGACCTCTTGCATGCCATGCAAGCGCTCTCCCAGc > 1:793584/1‑125 (MQ=255) ttACAAAAGCGGCAAAAAGCAGAGACAAAAA‑ACCCCCCGCTTTGCAGCGAGGGTTGGAAATTTGGTGGAGCTAAGCGGGATCGAACCGCTGACCTCTTGCATGCCATGCAAGCGCTCTCCCAGCTGAGCTATAGCCCCAc > 2:901208/1‑140 (MQ=255) tACAAAAGCGGCAAAAAGCAGAGACAAAAA‑ACCCCCCGCTTTGCAGCGAGGGTTGGAAATTTGGTGGAGCTAAGCGGGATCGAACCGCTGACCTCTTGCATGCCATGCAAGCGCTCTCCCAGCTGAGCTATAGCCCCACg < 1:901208/140‑1 (MQ=255) aCAAAAGCGGCAAAAAGCAGAGACAAAAA‑ACCCCCCGCTTTGCAGCGAGGGTTGGAAATTTGGTGGAGCTAAGCGGGATCGAACCGCTGACCTCTTGCATGCCATGCAAGCGCTCTCCCAGCTGAGCTATAGCCCCACGa < 2:460873/140‑1 (MQ=255) aaaaGCGGCAAAAAGCAGAGACAAAAA‑ACCCCCCGCTTTGCAGCGAGGGTTGGAAATTTGGTGGAGCTAAGCGGGATCGAACCGCTGACCTCTTGCATGCCATGCAAGCGCTCTCCCAGCTGAGCTATAGCCCCACGATg < 2:683020/140‑1 (MQ=255) aaaaGCGGCAAAAAGCAGAGACAAAAA‑ACCCCCCGCTTTGCAGCGAGGGTTGGAAATTTGGTGGAGCTAAGCGGGATCGAACCGCTGACCTCTTGCATGCCATGCAAGCGCTCTCCCAGCTGAGCTATAGCCCCACGATg < 1:865477/140‑1 (MQ=255) gCGGCAAAAAGCAGAGACAAAAA‑ACCCCCCGCTTTGCAGCGAGGGTTGGAAATTTGGTGGAGCTAAGCGGGATCGAACCGCTGACCTCTTGCATGCCATGCAAGCGCTCTCCCAGCTGAGCTATAGCCCCAc > 2:590844/1‑132 (MQ=255) gCGGCAAAAAGCAGAGACAAAAA‑ACCCCCCGCTTTGCAGCGAGGGTTGGAAATTTGGTGGAGCTAAGCGGGATCGAACCGCTGACCTCTTGCATGCCATGCAAGCGCTCTCCCAGCTGAGCTATAGCCCCAc < 1:590844/132‑1 (MQ=255) gCGGCAAAAAGCAGAGACAAAAA‑ACCCCCCGCTTTGCAGCGAGGGTTGGAAATTTGGTGGAGCTAAGCGGGATCGAACCGCTGACCTCTTGCATGCCATGCAAGCGCTCTCCCAGCTGAGCTATAGCCCCACGATGCGtt > 1:549281/1‑140 (MQ=255) gCGGCAAAAAGCAGAGACAAAAA‑ACCCCCCGCTTTGCAGCGAGGGTTGGAAATTTGGTGGAGCTAAGCGGGATCGAACCGCTGACCTCTTGCATGCCATGCAAGCGCTCTCCCAGCTGAGCTATAGCCCCACGATGCGtt > 2:459292/1‑140 (MQ=255) gCGGCAAAAAGCAGAGACAAAAA‑ACCCCCCGCTTTGCAGCGAGGGTTGGAAATTTGGTGGAGCTAAGCGGGATCGAACCGCTGACCTCTTGCATGCCATGCAAGCGCTCTCCCAGCTGAGCTATAGCCCCACGATGCGtt > 2:913454/1‑140 (MQ=255) aaaGCAGAGACAAAAA‑ACCCCCCGCTTTGCAGCGAGGGTTGGAAATTTGGTGGAGCTAAGCGGGATCGAACCGCTGACCTCTTGCATGCCATGCAAGCGCTCTCCCAGCTGAGCTATAGCCCCACGATGCGTTTACGTAc < 2:549281/140‑1 (MQ=255) caaaaaa‑c‑CCCCCGCTTTGCAGCGAGGGTTGGAAATTTGGTGGAGCTAAGCGGGATCGAACCGCTGACCTCTTGCATGCCATGCAAGCGCTCTCCCAGCTGAGCTATAGCCCCACGATGCGTTTACGTACCAAGTTTGCt < 2:814944/138‑1 (MQ=255) | AATAAGTTTATTCGCATCGCGAAGCAAACAGGGGGCCAGCCTGTTGCCGATTTTTCATCCAATGATGCTTTCACCAGGCGGCACTAACCCGGACGTAGACTGTATTACAAAAGCGGCAAAAAGCAGAGACAAAAA‑A‑CCCCCGCTTTGCAGCGAGGGTTGGAAATTTGGTGGAGCTAAGCGGGATCGAACCGCTGACCTCTTGCATGCCATGCAAGCGCTCTCCCAGCTGAGCTATAGCCCCACGATGCGTTTACGTACCAAGTTTGCT > NC_000913/2517876‑2518143 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |