| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,168,966 | 0 | A | C | 30.9% | 100.8 / 17.4 | 55 | I85I (ATA→ATC) | yegS | lipid kinase |
| Reads supporting (aligned to +/- strand): ref base A (30/8); new base C (0/17); total (30/25) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.58e-08 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
TCACCTGGGAGAAAGGCGATGCCGCACGATATGTAGAGGAGGCCCGGAAGTTTGGCGTCGCAACGGTGATTGCCGGTGGTGGCGATGGCACCATTAATGAAGTTTCTACGGCGTTGATTCAGTGTGAGGGGGATGACATACCCGCGCTGGGAATTTTGCCATTAGGAA‑CCGCCAATGATTTTGCCACCAGTGTAGGGATTCCTGAGGCACTGGATAAGGCGCTGAAACTGGCAATTGCCGGTGACGCCATTGCGATAGATATGGCGCAGGTCAACAAAC > NC_000913/2168827‑2169105 | tCACCTGGGAGAAAGGCGATGCCGCACGATATGTAGAGGAGGCCCGGAAGTTTGGCGTCGCAACGGTGATTGCCGGTGGTGGCGATGGCACCATTAATGAAGTTTCTACGGCGTTGATTCAGTGTGAGGGGGATGACATa > 1:137280/1‑140 (MQ=255) ccTGGGAGAAAGGCGATGCCGCACGATATGTAGAGGAGGCCCGGAAGTTTGGCGTCGCAACGGTGATTGCCGGTGGTGGCGATGGCACCATTAATGAAGTTTCTACGGCGTTGATTCAGTGTGAGGGGGATGACATAccc < 2:379042/140‑1 (MQ=255) aaGGCGATGCCGCACGATATGTAGAGGAGGCCCGGAAGTTTGGCGTCGCAACGGTGATTGCCGGTGGTGGCGATGGCACCATTAATGAAGTTTCTACGGCGTTGATTCAGTGTGAGGGGGATGACATACCCGCGCTGGGa > 2:548366/1‑140 (MQ=255) aGGCGATGCCGCACGATATGTAGAGGAGGCCCGGAAGTTTGGCGTCGCAACGGTGATTGCCGGTGGTGGCGATGGCACCATTAATGAAGTTTCTACGGCGTTGATTCAGTGTGAGGGGGATGACATACCCGCGCTGGGaa > 1:798284/1‑140 (MQ=255) aGGCGATGCCGCACGATATGTAGAGGAGGCCCGGAAGTTTGGCGTCGCAACGGTGATTGCCGGTGGTGGCGATGGCACCATTAATGAAGTTTCTACGGCGTTGATTCAGTGTGAGGGGGATGACATACCCGCGCTGGGaa > 1:756766/1‑140 (MQ=255) ggCGATGCCGCACGATATGTAGAGGAGGCCCGGAAGTTTGGCGTCGCAACGGTGATTGCCGGTGGTGGCGATGGCACCATTAATGAAGTTTCTACGGCGTTGATTCAGTGTGAGGGGGATGACATACCCGCGCTGGGAAt > 1:555128/1‑140 (MQ=255) cGATGCCGCACGATATGTAGAGGAGGCCCGGAAGTTTGGCGTCGCAACGGTGATTGCCGGTGGTGGCGATGGCACCATTAATGAAGTTTCTACGGCGTTGATTCAGTGTGAGGGGGATGACATACCCGCGCTGGGAAttt < 1:548366/140‑1 (MQ=255) aTGCCGCACGATATGTAGAGGAGGCCCGGAAGTTTGGCGTCGCAACGGTGATTGCCGGTGGTGGCGATGGCACCATTAATGAAGTTTCTACGGCGTTGATTCAGTGTGAGGGGGATGACATACCCGCGCTGGGAAttt < 1:268562/138‑1 (MQ=255) aTGCCGCACGATATGTAGAGGAGGCCCGGAAGTTTGGCGTCGCAACGGTGATTGCCGGTGGTGGCGATGGCACCATTAATGAAGTTTCTACGGCGTTGATTCAGTGTGAGGGGGATGACATACCCGCGCTGGGAAttt > 2:268562/1‑138 (MQ=255) aTGCCGCACGATATGTAGAGGAGGCCCGGAAGTTTGGCGTCGCAACGGTGATTGCCGGTGGTGGCGATGGCACCATTAATGAAGTTTCTACGGCGTTGATTCAGTGTGAGGGGGATGACATACCCGCGCTGGGAATTTTg < 2:137280/140‑1 (MQ=255) tatGTAGAGGAGGCCCGGAAGTTTGGCGTCGCAACGGTGATTGCCGGTGGTGGCGATGGCACCATTAATGAAGTTTCTACGGCGTTGATTCAGTGTGAGGGGGATGACATCCCCGCGCTGGGAATTTTGCCATTAGGAA‑c < 2:798284/140‑1 (MQ=255) ggaggCCGGGAAGTTTGGCGTCGAAACGGTGATTGCGGGTGGTGGCGATGGCACCATTAATGAAGTTTCTACGGCGTTGATTCAGTGTGAGGGGGATGACATCCCCGCGCGGGGATTTTTGCCATTAGGAA‑CCGCCAATg < 2:453037/140‑1 (MQ=255) ggaggCCCGGAAGTTTGGCGTCGCAACGGTGATTGCGGGTGGTGGCGATGGCACAATTAATGAAGTTTCTACGGCGTTGATTCAGTGTGGGGGGGATGACATCCCCGCGCTGGGAATTTTGCCATTAGGAA‑CCGCCAATg < 2:532594/140‑1 (MQ=255) ggaggCCCGGAAGTTTGGCGTCGCAACGGTGATTGCCGGTGGTGGCGATGGCACCATTAATGAAGTTTCTACGGCGTTGATTCAGTGTGAGGGGGATGACATACCCGCGCTGGGAATTTTGCCATTAGGAA‑CCGCCAATg > 1:563477/1‑140 (MQ=255) aggCCCGGAAGTTTGGCGTCGCAACGGTGATTGCCGGTGGTGGCGATGGTACCATTAATGAAGTTTCTACGGCGTTGATTCAGTGTGAGGGGGATGACATACCCGCGCTGGGAATTTTGCCATTAGGAA‑CCGCCAATGAt > 1:54979/1‑140 (MQ=255) aggCCCGGAAGTTTGGCGTCGCAACGGTGATTGCCGGTGGTGGCGATGGCACCATTAATGAAGTTTCTACGGCGTTGATTCAGTGTGAGGGGGATGACATACCCGCGCTGGGAATTTTGCCATTAGGAA‑CCGCCAATGAt > 1:488865/1‑140 (MQ=255) aggCCCGGAAGTTTGGCGTCGCAACGGTGATTGCCGGTGGTGGCGATGGCACCATTAATGAAGTTTCTACGGCGTTGATTCAGTGTGAGGGGGATGACATACCCGCGCTGGGAATTTTGCCATTAGGAA‑CCGCCAATGAt > 1:576460/1‑140 (MQ=255) aggCCCGGAAGTTTGGCGTCGCAACGGTGATTGCCGGTGGTGGCGATGGCACCATTAATGAAGTTTCTACGGCGTTGATTCAGTGTGAGGGGGATGACATACCCGCGCTGGGAATTTTGCCATTAGGAA‑CCGCCAATGAt > 1:644265/1‑140 (MQ=255) aggCCCGGAAGTTTGGCGTCGCAACGGTGATTGCCGGTGGTGGCGATGGCACCATTAATGAAGTTTCTACGGCGTTGATTCAGTGTGAGGGGGATGACATACCCGCGCTGGGAATTTTGCCATTAGGAA‑CCGCCAATGAt > 2:797767/1‑140 (MQ=255) gCCCGGAAGTTTGGCGTCGCAACGGTGATTGCCGGTGGTGGCGATGGCACCATTAATGAAGTTTCTACGGCGTTGATTCAGTGTGAGGGGGATGACATACCCGCGCTGGGAATTTTGCCATTAGGAA‑CCGCCAATGAttt > 2:342309/1‑140 (MQ=255) gCCCGGAAGTTTGGCGTCGCAACGGTGATTGCCGGTGGTGGCGATGGCACCATTAATGAAGTTTCTACGGCGTTGATTCAGTGTGAGGGGGATGACATACCCGCGCTGGGAATTTTGCCATTAGGAA‑CCGCCAATGAttt > 1:323808/1‑140 (MQ=255) gCCCGGAAGTTTGGCGTCGCAACGGTGATTGCCGGTGGTGGCGATGGCACCATTAATGAAGTTTCTACGGCGTTGATTCAGTGTGAGGGGGATGACATACCCGCGCTGGGAATTTTGCCATTAGGAA‑CCGCCAATGAttt > 1:8463/1‑140 (MQ=255) cccGGAAGTTTGGCGTCGCAACGGTGATTGCCGGTGGTGGCGATGGCACCATTAATGAAGTTTCTACGGCGTTGATTCAGTGTGAGGGGGATGACATACCCGCGCTGGGAATTTTGCCATTAGGAA‑CCGCCAATGAtttt < 1:797767/140‑1 (MQ=255) cccGGAAGTTTGGCGTCGCAACGGTGATTGCCGGTGGTGGCGATGGCACCATTAATGAAGTTTCTACGGCGTTGATTCAGTGTGAGGGGGATGACATACCCGCGCTGGGAATTTTGCCATTAGGAA‑CCGCCAATGAtttt > 2:315246/1‑140 (MQ=255) cGGAAGTTTGGCGTCGCAAGGGTGATTGCCGGTGGTGGCGAGGGCCCCATTAATGAAGTTTCTACGGCGTTGATTCAGTGTGAGGGGGATGACATCCCCGCGCGGGGAATTTTGCCATTAGGAA‑CCGCCAATGATTTTGc < 2:777573/140‑1 (MQ=255) gAAGTTTGGCGTCGCAACGGTGATTGCCGGTGGTGGCGATGGCACCATTAATGAAGTTTCTACGGCGTTGATTCAGTGTGAGGGGGATGACATACCCGCGCTGGGAATTTTGCCATTAGGAA‑CCGCCAATGATTTTGcca > 1:777573/1‑140 (MQ=255) aaGTTTGGCGTCGCAACGGTGTTTGCCGGTGGTGGCGATGGCACCATTAATGAAGTTTCTACGGCGTTGATTCAGTGTGAGGGGGATGACATCCCCGCGCTGGGAATTTTGCCATTAGGAA‑CCGCCAATGATTTTGccac < 2:358254/140‑1 (MQ=255) aaGTTTGGCGTCGCAACGGTGATTGCCGGTGGTGGCGATGGTACCATTAATGAAGTTTCTACGGCGTTGATTCAGTGTGAGGGGGATGACATCCCCGCGCTGGGAATTTTGCCATTAGGAA‑CCGCCAATGATTTTGccac < 2:54979/140‑1 (MQ=255) aaGTTTGGCGTCGCAACGGTGATTGCCGGTGGTGGCGATGGCACCTTTAATGAAGTTTCTACGGCGTTGATTCAGTGTGAGGGGGATGACATACCCGCGCTGGGAATTTTGCCATTAGGAA‑CCGCCAATGATTTTGccac < 2:488865/140‑1 (MQ=255) aaGTTTGGCGTCGCAACGGTGATTGCCGGTGGTGGCGATGGCACCATTAATGAAGTTTCTACGGCGTTGATTCAGTGTGAGGGGGATGACATCCCCGCGCTGGGAATTTTGCCATTAGGAA‑CCGCCAATGATTTTGccac < 1:212277/140‑1 (MQ=255) aaGTTTGGCGTCGCAACGGTGATTGCCGGTGGTGGCGATGGCACCATTAATGAAGTTTCTACGGCGTTGATTCAGTGTGAGGGGGATGACATCCCCGCGCTGGGAATTTTGCCATTAGGAA‑CCGCCAATGATTTTGccac < 1:342309/140‑1 (MQ=255) aaGTTTGGCGTCGCAACGGTGATTGCCGGTGGTGGCGATGGCACCATTAATGAAGTTTCTACGGCGTTGATTCAGTGTGAGGGGGATGACATCCCCGCGCTGGGAATTTTGCCATTAGGAA‑CCGCCAATGATTTTGccac < 1:822984/140‑1 (MQ=255) aaGTTTGGCGTCGCAACGGTGATTGCCGGTGGTGGCGATGGCACCATTAATGAAGTTTCTACGGCGTTGATTCAGTGTGAGGGGGATGACATCCCCGCGCGGGGATTTTTGCCATTAGGAA‑CCGCCAATGATTTTGccac < 2:402468/140‑1 (MQ=255) aaGTTGGGCGTCGCAGCGGTGATTGCCGGTGGCGGCGATGGCCCCATTAATAAAGTTTCTACGGCGTTGATTCAGGGTGAGGGGGATGACATCCCCGCCGTGGGAATTTTCCCATTAGGAA‑CCGCCAATGATTTTGccac < 2:576460/140‑1 (MQ=255) ttGGCGTCGCAACGGTGATTGCCGGTGGTGGCGATGGCACCATTAATGAAGTTTCTACGGCGTTGATTCAGTGTGAGGGGGATGACATACCCGCGCTGGGAATTTTGCCATTAGGAA‑CCGCCAATGATTTTGCCACCAgt > 2:535360/1‑140 (MQ=255) cAACGGTGATTGCCGGTGGTGGCGATGGCACCATTAATGAAGTTTCTACGGCGTTGATTCAGTGTGAGGGGGATGACATACCCGCGCTGGGAATTTTGCCATTAGGAA‑CCGCCAATGATTTTGCCACCAGTGTAGGGAtt > 1:533139/1‑140 (MQ=255) tGATTGCCGGTGGTGGCGATGGCACCATTAATGAAGTTTCTACGGCGTTGATTCAGTGTGAGGGGGATGACATACCCGCGCTGGGAATTTTGCCATTAGGAA‑CCGCCAATGATTTTGCCACCAGTGTAGGGATTCCTGAg < 2:533139/140‑1 (MQ=255) tGATTCCCGGTGGGGGCGTGGCCACCATTAATGAAGTTTCTACGGCGTTGATTGAGTGTGAGGGGGATGAAATCCCCGCCGGGGAAATTTTGCCATGGGCAA‑CCGCCAATGATTTTGCCACCAGTGTAGGGATTCCTGAg < 2:8463/140‑1 (MQ=255) gtggtgGCGATGGCACCATTAATGAAGTTTCTACGGCGTTGATTCAGTGTGAGGGGGATGACATACCCGCGCTGGGAATTTTGCCATTAGGAA‑CCGCCAATGATTTTGCCACCAGTGTAGGGATTCCTGAGGCACTGGAt > 1:585525/1‑140 (MQ=255) cGATGGCACCATTAATGAAGTTTCTACGGCGTTGATTCAGTGTGAGGGGGATGACATACCCGCGCTGGGAATTTTGCCATTAGGAA‑CCGCCAATGATTTTGCCACCAGTGTAGGGATTCCTGAGGCACTGGATAAGgcgc > 2:805460/1‑140 (MQ=255) aaTGAAGTTTCTAGGGCGTTGTTTCATTGTGAGGGGGATGACTTGCCGGGGGT‑GGAAGTTTTCCTTTAAGAATCCGCCAATGATTTTGCCACCAGTGTAGGGATTCCTGAGGCACTGGATAAGGCGCTGAAACTGGCAAt < 2:36516/140‑1 (MQ=255) gTTTCTACGGCGTTGATTCAGTGTGAGGGGGATGACATCCCCGCGCTGGGAATTTTGCCATTAGGAA‑CCGCCAATGATTTTGCCACCAGTGTAGGGATTCCTGAGGCACTGGATAAGGCGCTGAAACTGGCAATTGCCgg < 2:435771/140‑1 (MQ=255) gTTTCTACGGCGTTGATTCAGTGTGAGGGGGATGACATACCCGCGCTGGGAATTTTGCCATTAGGAA‑CCGCCAATGATTTTGCCACCAGTGTAGGGATTCCTGAGGCACTGGATAAGGCGCTGAAACTGGCAATTGCCgg > 1:624545/1‑140 (MQ=255) ttCTACGGCGTTGATTCAGTGTGAGGGGGATGACATACCCGCGCTGGGAATTTTGCCATTAGGAA‑CCGCCAATGATTTTGCCACCAGTGTAGGGATTCCTGAGGCACTGGATAAGGCGCTGAAACTGGCAATTGCCGGTg > 1:36516/1‑140 (MQ=255) ttCTACGGCGTTGATTCAGTGTGAGGGGGATGACATACCCGCGCTGGGAATTTTGCCATTAGGAA‑CCGCCAATGATTTTGCCACCAGTGTAGGGATTCCTGAGGCACTGGATAAGGCGCTGAAACTGGCAATTGCCGGTg > 2:42327/1‑140 (MQ=255) gcGGCGTTGATTCAGTGGGGGGGGGATGACATCCCCGCGCGGGGATTTTTGCCATTAGGAA‑CCGCCAATGATTTTGCCACCAGTGTAGGGATTCCTGAGGCACTGGATAAGGCGCTGAAACTGGCAATTGCCGGTGACGc < 2:922482/139‑1 (MQ=255) cGGCGTTGATTCAGTGTGAGGGGGATGACATCCCCGCGCTGGGAATTTTGCCATTAGGAA‑CCGCCAATGATTTTGCCACCAGTGTAGGGATTCCTGAGGCACTGGATAAGGCGCTGAAACTGGCAATTGCCGGTGACGcc < 1:805460/140‑1 (MQ=255) ggCGTTGATTCAGTGTGAGGGGGATGACATCCCCGCGCTGGGAATTTTGCCATTAGGAA‑CCGCCAATGATTTTGCCACCAGTGTAGGGATTCCTGAGGCACTGGATAAGGCGCTGAAACTGGCAATTGCCGGTGACGCCa < 2:563477/140‑1 (MQ=255) ttGATTCAGTGTGAGGGGGATGACATCCCCGCGCTGGGAATTTTGCCATTAGGAA‑CCGCCAATGATTTTGCCACCAGTGTAGGGATTCCTGAGGCACTGGATAAGGCGCTGAAACTGGCAATTGCCGGTGACGCCATTGc < 2:323808/140‑1 (MQ=255) tGATTCAGTGTGGGGGGGATGACATCCCCGCGCGGGGAATTTTGCCATTAGAAA‑CCGCCAATGATTTTGCCACCAGTGTAGGGATTCCTGAGGCACTGGATAAGGCGCTGAAACTGGCAATTGCCGGTGACGCCATTGCg < 1:448014/140‑1 (MQ=255) gtgtGAGGGGGATGACATCCCCGCGCTGGGAATTTTGCCATTAGGAA‑CCGCCAATGATTTTGCCACCAGTGTAGGGATTCCTGAGGCACTGGATAAGGCGCTGAAACTGGCAATTGCCGGTGACGCCATTGCgatagata < 2:585525/140‑1 (MQ=255) gtgtGAGGGGGATGACATACCCGCGCTGGGAATTTTGCCATTAGGAA‑CCGCCAATGATTTTGCCACCAGTGTAGGGATTCCTGAGGCACTGGATAAGGCGCTGAAACTGGCAATTGCCGGTGACGCCATTGCgatagata < 1:315246/140‑1 (MQ=255) aGGGGGATGACATACCCGCGCTGGGAATTTTGCCATTAGGAA‑CCGCCAATGATTTTGCCACCAGTGTAGGGATTCCTGAGGCACTGGATAAGGCGCTGAAACTGGCAATTGCCGGTGACGCCATTGCGATAGATATGgcg > 1:48303/1‑140 (MQ=255) aGGGGGATGACATACCCGCGCTGGGAATTTTGCCATTAGGAA‑CCGCCAATGATTTTGCCACCAGTGTAGGGATTCCTGAGGCACTGGATAAGGCGCTGAAACTGGCAATTGCCGGTGACGCCATTGCGATAGATATGgcg > 2:128152/1‑140 (MQ=255) gggggATGACATCCCCGCGCTGGGAATTTTGCCATTAGGAA‑CCGCCAATGATTTTGCCACCAGTGTAGGGATTCCTGAGGCACTGGATAAGGCGCTGAAACTGGCAATTGCCGGTGACGCCATTGCGATAGATATGgcgc < 1:535360/140‑1 (MQ=255) gggggATGACATACCCGCGCTGGGAATTTTGCCATTAGGAA‑CCGCCAATGATTTTGCCACCAGTGTAGGGATTCCTGAGGCACTGGATAAGGCGCTGAAACTGGCAATTGCCGGTGACGCCATTGCGATAGATATGgcgc > 1:805650/1‑140 (MQ=255) gATGACATACCCGCGCTGGGAATTTTGCCATTAGGAA‑CCGCCAATGATTTTGCCACCAGTGTAGGGATTCCTGAGGCACTGGATAAGGCTCTGAAACTGGCAATTGCCGGTGACGCCATTGCGATAGATATGGCGCAGGt > 1:875050/1‑140 (MQ=255) aTGACATACCCGCGCTGGGAATTTTGCCATTAGGAA‑CCGCCAATGATTTTGCCACCAGTGTAGGGATTCCTGAGGCACTGGATAAGGCGCTGAAACTGGCAATTGCCGGTGACGCCATTGCGATAGATATGGCGCAGGTc > 1:394709/1‑140 (MQ=255) gACATACCCGCGCTGGGAATTTTGCCATTAGGAA‑CCGCCAATGATTTTGCCACCAGTGTAGGGATTCCTGAGGCACTGGATAAGGCGCTGAAACTGGCAATTGCCGGTGACGCCATTGCGATAGATATGGCGCAGGTcaa > 1:834807/1‑140 (MQ=255) tACCCGCGCTGGGAATTTTGCCATTAGGAA‑CCGCCAATGATTTTGCCACCAGTGTAGGGATTCCTGAGGCACTGGATAAGGCGCTGAAAGTGGCAATTGCCGGTGACGCCATTGCGATAGATATGGCGCAGGTCaacaaa > 2:868195/1‑140 (MQ=255) aCCCGCGCTGGGAATTTTGCCATTAGGAA‑CCGCCAATGATTTTGCCACCAGTGTAGGGATTCCTGAGGCACTGGATAAGGCGCTGAAACTGGCAATTGCCGGTGACGCCATTGCGATAGATATGGCGCAGGTCaacaaac > 1:314223/1‑140 (MQ=255) | TCACCTGGGAGAAAGGCGATGCCGCACGATATGTAGAGGAGGCCCGGAAGTTTGGCGTCGCAACGGTGATTGCCGGTGGTGGCGATGGCACCATTAATGAAGTTTCTACGGCGTTGATTCAGTGTGAGGGGGATGACATACCCGCGCTGGGAATTTTGCCATTAGGAA‑CCGCCAATGATTTTGCCACCAGTGTAGGGATTCCTGAGGCACTGGATAAGGCGCTGAAACTGGCAATTGCCGGTGACGCCATTGCGATAGATATGGCGCAGGTCAACAAAC > NC_000913/2168827‑2169105 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |