Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	19,615	C→T	R301C (CGT→TGT)	nhaR →	DNA‑binding transcriptional activator NhaR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	19,615	0	C	T	100.0%	223.1 / NA	64	R301C (CGT→TGT)	nhaR	DNA‑binding transcriptional activator NhaR
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (30/34);  total (30/34)

TCGTAGAAATTGGTCGCGTCGAGAATGTGATGGAAGAGTACCATGCTATTTTTGCTGAGCGGATGATTCAGCACCCGGCGGTACAGCGAATCTGCAATACGGATTATTCTGCGCTTTTTAGTCCAGCGGTGCGTTAATCGGCAGCTCCCCCAAAGTTAAGGTGGGGGAGATAGATTAGTTGTACATTACCACGATTTTGACTCGGCTCATTATTTGCCCGCTTGAGACATTGTTTCCATATGTACGCGGGCGAATAAATAGAGGAAT  >  NC_000913/19484‑19750                                                                                                                                    |                                                                                                                                        tCGTAGAAATTGGTCGCGTCGAGAATGTGATGGAAGAGTACCATGCTATTTTTGCTGAGCGGATGATTCAGCACCCGGCGGTACAGCGAATCTGCAATACGGATTATTCTGCGCTTTTTAGTCCAGCGGTGTGTTAATCg                                                                                                                                 >  2:857073/1‑140 (MQ=255)  cGTAGAAATTGGTCGCGTCGAGAATGTGATGGAAGAGTACCATGCTATTTTTGCTGAGCGGATGATTCAGCACCCGGCGGTACAGCGAATCTGCAATACGGATTATTCTGCGCTTTTTAGTCCAGCGGTGTGTTAATCgg                                                                                                                                >  2:319806/1‑140 (MQ=255)     aGAAATTGGTCGCGTCGAGAATGTGATGGAAGAGTACCATGCTATTTTTGCTGAGCGGATGATTCAGCACCCGGCGGTACAGCGAATCTGCAATACGGATTATTCTGCGCTTTTTAGTCCAGCGGTGTGTTAATCGGCAg                                                                                                                             <  2:194855/140‑1 (MQ=255)     aGAAATTGGTCGCGTCGAGAATGTGATGGAAGAGTACCATGCTATTTTTGCTGAGCGGATGATTCAGCACCCGGCGGTACAGCGAATCTGCAATACGGATTATTCTGCGCTTTTTAGTCCAGCGGTGTGTTAATCGGCAg                                                                                                                             <  2:548189/140‑1 (MQ=255)           tGGTCGCGTCGAGAATGTGATGGAAGAGTACCATGCTATTTTTGCTGAGCGGATGATTCAGCACCCGGCGGTACAGTGAATCTGCAATACGGATTATTCTGCGCTTTTTAGTCCAGCGGTGTGTTAATCGGCAGCTcccc                                                                                                                       >  2:151311/1‑140 (MQ=255)           tGGTCGCGTCGAGAATGTGATGGAAGAGTACCATGCTATTTTTGCTGAGCGGATGATTCAGCACCCGGCGGTACAGCGAATCTGCAATACGGATTATTCTGCGCTTTTTAGTCCAGCGGTGTGTTAATCGGCAGCTcccc                                                                                                                       >  2:920421/1‑140 (MQ=255)           tGGTCGCGTCGAGAATGTGATGGAAGAGTACCATGCTATTTTTGCTGAGCGGATGATTCAGCACCCGGCGGTACAGCGAATCTGCAATACGGATTATTCTGCGCTTTTTAGTCCAGCGGTGTGTTAATCGGCAGCTcccc                                                                                                                       >  1:234397/1‑140 (MQ=255)           tGGTCGCGTCGAGAATGTGATGGAAGAGTACCATGCTATTTTTGCTGAGCGGATGATTCAGCACCCGGCGGTACAGCGAATCTGCAATACGGATTATTCTGCGCTTTTTAGTCCAGCGGTGTGTTAATCGGCAGCTcccc                                                                                                                       >  1:428413/1‑140 (MQ=255)            ggTCGCGTCGAGAATGTGATGGAAGAGTACCATGCTATTTTTGCTGAGCGGATGATTCAGCACCCGGCGGTACAGCGAATCTGCAATACGGATTATTCTGCGCTTTTTAGTCCAGCGGTGTGTTAATCGGCAGCTccccc                                                                                                                      <  2:428413/140‑1 (MQ=255)               cgcgTCGAGAATGTGATGGAAGAGTACCATGCTATTTTTGCTGAGCGGATGATTCAGCACCCGGCGGTACAGCGAATCTGCAATACGGATTATTCTGCGCTTTTTAGTCCAGCGGTGTGTTAATCGGCAGCTCCCCCaaa                                                                                                                   >  2:100135/1‑140 (MQ=255)                     gagaATGTGATGGAAGAGTACCATGCTATTTTTGCTGAGCGGATGATTCAGCACCCGGCGGTACAGCGAATCTGCAATACGGATTATTCTGCGCTTTTTAGTCCAGCGGTGTGTTAATCGGCAGCTCCCCCAAAGTTAAg                                                                                                             <  1:100135/140‑1 (MQ=255)                     gagaATGTGATGGAAGAGTACCATGCTATTTTTGCTGAGCGGATGATTCAGCACCCGGCGGTACAGCGAATCTGCAATACGGATTATTCTGCGCTTTTTAGTCCAGCGGTGTGTTAATCGGCAGCTCCCCCAAAGTTAAg                                                                                                             <  1:319806/140‑1 (MQ=255)                     gagaATGTGATGGAAGAGTACCATGCTATTTTTGCTGAGCGGATGATTCAGCACCCGGCGGTACAGCGAATCTGCAATACGGATTATTCTGCGCTTTTTAGTCCAGCGGTGTGTTAATCGGCAGCTCCCCCAAAGTTAAg                                                                                                             <  1:266189/140‑1 (MQ=255)                          tgtgATGGAAGAGTACCATGCTATTTTTGCTGAGCGGATGATTCAGCACCCGGCGGTACAGCGAATCTGCAATACGGATTATTCTTCGCTTTTTAGTCCAGCGGTGTGTTAATCGGCAGCTCCCCCAAAGTTAAGGTggg                                                                                                        <  1:863010/140‑1 (MQ=255)                          tgtgATGGAAGAGTACCATGCTATTTTTGCTGAGCGGATGATTCAGCACCCGGCGGTACAGCGAATCTGCAATACGGATTATTCTGCGCTTTTTAGTCCAGCGGTGTGTTAATCGGCAGCTCCCCCAAAGTTAAGGTggg                                                                                                        <  1:857073/140‑1 (MQ=255)                            tgATGGAAGAGTACCATGCTATTTTTGCTGAGCGGATGATTCAGCACCCGGCGGTACAGCGAATCTGCAATACGGATTATTCTGCGCTTTTTAGTCCAGCGGTGTGTTAATCGGCAGCTCCCCCAAAGTTAAGGTggggg                                                                                                      >  1:750432/1‑140 (MQ=255)                                  aaGAGTACCATGCTATTTTTGCTGAGCGGATGATTCAGCACCCGGCGGTACAGCGAATCTGCAATACGGATTATTCTGCGCTTTTTAGTCCAGCGGTGTGTTAATCGGCAGCTCCCCCAAAGTTAAGGTGGGGGagatag                                                                                                >  1:316285/1‑140 (MQ=255)                                  aaGAGTACCATGCTATTTTTGCTGAGCGGATGATTCAGCACCCGGCGGTACAGCGAATCTGCAATACGGATTATTCTGCGCTTTTTAGTCCAGCGGTGTGTTAATCGGCAGCTCCCCCAAAGTTAAGGTGGGGGagatag                                                                                                <  1:784492/140‑1 (MQ=255)                                    gagTACCATGCTATTTTTGCTGAGCGGATGATTCAGCACCCGGCGGTACAGCGAATCTGCAATACGGATTATTCTGCGCTTTTTAGTCCAGCGGTGTGTTAATCGGCAGCTCCCCCAAAGtt                                                                                                                <  2:816296/122‑1 (MQ=255)                                    gagTACCATGCTATTTTTGCTGAGCGGATGATTCAGCACCCGGCGGTACAGCGAATCTGCAATACGGATTATTCTGCGCTTTTTAGTCCAGCGGTGTGTTAATCGGCAGCTCCCCCAAAGtt                                                                                                                >  1:816296/1‑122 (MQ=255)                                    gagTACCATGCTATTTTTGCTGAGCGGATGATTCAGCACCCGGCGGTACAGCGAATCTGCAATACGGATTATTCTGCGCTTTTTAGTCCAGCGGTGTGTTAATCGGCAGCTCCCCCAAAGTTAAGGTGGGGGagatagat                                                                                              <  2:234397/140‑1 (MQ=255)                                    gagTACCATGCTATTTTTGCTGAGCGGATGATTCAGCACCCGGCGGTACAGCGAATCTGCAATACGGATTATTCTGCGCTTTTTAGTCCAGCGGTGTGTTAATCGGCAGCTCCCCCAAAGTTAAGGTGGGGGagatagat                                                                                              <  1:878065/140‑1 (MQ=255)                                      gTACCATGCTATTTTTGCTGAGCGGATGATTCAGCACCCGGCGGTACAGCGAATCTGCAATACGGATTATTCTGCGCTTTTTAGTCCAGCGGTGTGTTAATCGGCAGCTCCCCCAAAGTTAAGGTGGGGGAGATAGATTa                                                                                            >  1:591514/1‑140 (MQ=255)                                      gTACCATGCTATTTTTGCTGAGCGGATGATTCAGCACCCGGCGGTACAGCGAATCTGCAATACGGATTATTCTGCGCTTTTTAGTCCAGCGGTGTGTTAATCGGCAGCTCCCCCAAAGTTAAGGTGGGGGAGATAGATTa                                                                                            >  1:276705/1‑140 (MQ=255)                                       tACCATGCTATTTTTGCTGAGCGGATGATTCAGCACCCGGCGGTACAGCGAATCTGCAATACGGATTATTCTGCGCTTTTTAGTCCAGCGGTGTGTTAATCGGCAGCTCCCCCAAAGTTAAGGTGGGGGAGATAGATTAg                                                                                           >  1:492726/1‑140 (MQ=255)                                       tACCATGCTATATTTGCTGAGCGGATGATTCAGCACCCGGCGGTACAGCGAATCTGCAATACGGATTATTCTGCGCTTTTTAGTCCAGCGGTGTGTTAATCGGCAGCTCCCCCGAAGTTAAGGTGGGGGAGATAGATTAg                                                                                           >  1:680270/1‑140 (MQ=255)                                         ccATGCTATTTTTGCTGAGCGGATGATTCAGCACCCGGCGGTACAGTGAATCTGCAATACGGATTATTCTGCGCTTTTTAGTCCAGCGGTGTGTTAATCGGCAGCTCCCCCAAAGTTAAGGTGGGGGAGATAGATTAGtt                                                                                         <  1:151311/140‑1 (MQ=255)                                         ccATGCTATTTTTGCTGAGCGGATGATTCAGCACCCGGCGGTACAGCGAATCTGCAATACGGATTATTCTGCGCTTTTTAGTCCAGCGGTGTGTTAATCGGCAGCTCCCCCAAAGTTAAGGTGGGGGAGATAGATTAGtt                                                                                         <  1:920421/140‑1 (MQ=255)                                           aTGCTATTTTTGCTGAGCGGATGATTCAGCACCCGGCGGTACAGCGAATCTGCAATACGGATTATTCTGCGCTTTTTAGTCCAGCGGTGTGTTAATCGGCAGCTCCCCCAAAGTTAAGGTGGGGGAGATAGATTAGTTGt                                                                                       >  2:213832/1‑140 (MQ=255)                                                                      aGCACCCGGCGGTACAGCGAATCTGCAATACGGATTATTCTGCGCTTTTTAGTCCAGCGGTGTGTTAATCGGCAGCTCCCCCAAAGTTAAGGTGGGGGAGATAGATTAGTTGTACATTACCACGATTTTGACTCGGCTCa                                                            >  1:710178/1‑140 (MQ=255)                                                                      aGCACCCGGCGGTACAGCGAATCTGCAATACGGATTATTCTGCGCTTTTTAGTCCAGCGGTGTGTTAATCGGCAGCTCCCCCAAAGTTAAGGTGGGGGAGATAGATTAGTTGTACATTACCACGATTTTGACTCGGCTCa                                                            >  2:713819/1‑140 (MQ=255)                                                                         aCCCGGCGGTACAGCGAATCTGCAATACGGATTATTCTGCGCTTTTTAGTCCAGCGGTGTGTTAATCGGCAGCTCCCCCAAAGTTAAGGTGGGGGAGATAGATTAGTTGTACATTACCACGATTTTGACTCGGCTCatta                                                         >  1:613949/1‑140 (MQ=255)                                                                             ggcggTACAGCGAATCTGCAATACGGATTATTCTGCGCTTTTTAGTCCAGCGGTGTGTTAATCGGCAGCTCCCCCAAAGTTAAGGTGGGGGAGATAGATTAGTTGTACATTACCACGATTTTGACTCGGCTCATTATTTg                                                     >  2:68646/1‑140 (MQ=255)                                                                              gcggTACAGCGAATCTGCAATACGGATTATTCTGCGCTTTTTAGTCCAGCGGTGTGTTAATCGGCAGCTCCCCCAAAGTTAAGGTGGGGGAGATAGATTAGTTGTACATTACCACGATTTTGACTCGGCTCATTATTTGc                                                    <  2:316285/140‑1 (MQ=255)                                                                                  tACAGCGAATCTGCAATACGGATTATTCTGCGCTTTTTAGTCCAGCGGTGTGTTAATCGGCAGCTCCCCCAAAGTTAAGGTGGGGGAGATAGATTAGTTGTACATTACCACGATTTTGACTCGGCTCatt                                                          <  1:35696/130‑1 (MQ=255)                                                                                  tACAGCGAATCTGCAATACGGATTATTCTGCGCTTTTTAGTCCAGCGGTGTGTTAATCGGCAGCTCCCCCAAAGTTAAGGTGGGGGAGATAGATTAGTTGTACATTACCACGATTTTGACTCGGCTCatt                                                          >  2:35696/1‑130 (MQ=255)                                                                                       cGAATCTGCAATACGGATTATTGTGCGCTTTTTAGTCCAGCGGTTTGGTAATCGGCCGCTCCCCCAAAGTTAAGGTGGGGGAGGTAGATTAGTTGTACCTTATCCCGATTTTGACTCGGCCCATTTTTTGGCCGCTTgga                                           >  2:708493/1‑138 (MQ=255)                                                                                       cGAATCTGCAATACGGATTATTCTGCGCTTTTTAGTCCAGCGGTGTGTTAATCGGCAGCTCCCCCAAAGTTAAGGTGGGGGAGATAGATTAGTTGTACATTACCACGATTTTGACTCGGCTCATTATTTGCCCGCTTgag                                           <  1:900962/140‑1 (MQ=255)                                                                                         aaTCTGCAATACGGATTATTCTGCGCTTTTTAGTCCAGCGGTGTGTTAATCGGCAGCTCCCCCAAAGTTAAGGTGGGGGAGATAGATTAGTTGTACATTACCACGATTTTGACTCGGCTCATTATTTGCCCGCTTGAGAc                                         >  2:744605/1‑140 (MQ=255)                                                                                         aaTCTGCAATACGGATTATTCTGCGCTTTTTAGTCCAGCGGTGTGTTAATCGGCAGCTCCCCCAAAGTTAAGGTGGGGGAGATAGATTAGTTGTACATTACCACGATTTTGACTCGGCTCATTATTTGCCCGCTTGAGAc                                         <  2:591514/140‑1 (MQ=255)                                                                                          aTCTGCAATACGGATTATTCTGCGCTTTTTAGTCCAGCGGTGTGTTAATCGGCAGCTCCCCCAAAGTTAAGGTGGGGGAGATAGATTAGTTGTACATTACCACGATTTTGACTCGGCTCATTATTTGCCCGCTTGAGACa                                        <  2:629474/140‑1 (MQ=255)                                                                                          aTCTGCAATACGGATTATTCTGCGCTTTTTAGTCCAGCGGTGTGTTAATCGGCAGCTCCCCCAAAGTTAAGGTGGGGGAGATAGATTAGTTGTACATTACCACGATTTTGACTCGGCTCATTATTTGCCCGCTTGAGACa                                        <  2:613949/140‑1 (MQ=255)                                                                                           tCTGCAATACGGATTATTCTGCGCTTTTTAGTCCAGCGGTGTGTTAATCGGCAGCTCCCCCAAAGTTAAGGTGGGGGAGATAGATTAGTTGTACATTACCACGATTTTGACTCGGCTCATTATTTGcc                                                   >  2:375243/1‑128 (MQ=255)                                                                                           tCTGCAATACGGATTATTCTGCGCTTTTTAGTCCAGCGGTGTGTTAATCGGCAGCTCCCCCAAAGTTAAGGTGGGGGAGATAGATTAGTTGTACATTACCACGATTTTGACTCGGCTCATTATTTGcc                                                   <  1:375243/128‑1 (MQ=255)                                                                                            cTGCAATACGGATTATTCTGCGCTTTTTAGTCCAGCGGTGTGTTAATCGGCAGCTCCCCCAAAGTTAAGGTGGGGGAGATAGATTAGTTGTACATTTCCACGATTTTGACTCGGCTCATTATTTGCCCGCTTGAGACAtt                                      <  1:708493/140‑1 (MQ=255)                                                                                                  tACGGATTATTCTGCGCTTTTTAGTCCAGCGGTGTGTTAATCGGCAGCTCCCCCAAAGTTAAGGTGGGGGAGATAGATTAGTTGTACATTACCACGATTTTGACTCGGCTCATTATTTGCCCGCTTGAGACATTGTTTcc                                <  2:492726/140‑1 (MQ=255)                                                                                                  tACGGATTATTCTGCGCTTTTTAGTCCAGCGGTGTGTTAATCGGCAGCCCCCCCAAAGTTAAGGTGGGGGAGATAGATTAGTTGTACATTACCACGATTTTGACTCGGCTCATTATTTGCCCGCTTGAGACATTGTTTcc                                <  2:658029/140‑1 (MQ=255)                                                                                                    cGGATTATTCTGCGCTTTTTAGTCCAGCGGTGTGTTAATCGGCAGCTCCCCCAAAGTTAAGGTGGGGGAGATAGATTAGTTGTACATTACCACGATTTTGACTCGGCTCATTATTTGCCCGCTTGAGACATTGTTTCCat                              <  2:276705/140‑1 (MQ=255)                                                                                                         tattCTGCGCTTTTTAGTCCAGCGGTGTGTTAATCGGCAGCTCCCCCAAAGTTAAGGTGGGGGAGATAGATTAGTTGTACATTACCACGATTTTGACTCGGCTCATTATTTGCCCGCTTGAGACATTGTTTCCat                              <  2:322914/135‑1 (MQ=255)                                                                                                         tattCTGCGCTTTTTAGTCCAGCGGTGTGTTAATCGGCAGCTCCCCCAAAGTTAAGGTGGGGGAGATAGATTAGTTGTACATTACCACGATTTTGACTCGGCTCATTATTTGCCCGCTTGAGACATTGTTTCCat                              >  1:322914/1‑135 (MQ=255)                                                                                                          attCTGCGCTTTTTAGTCCAGCGGTGTGTTAATCGGCAGCTCCCCCAAAGTTAAGGTGGGGGAGATAGATTAGTTGTACATTACCACGATTTTGACTCGGCTCATTATTTGCCCGCTTGAGACATTGTTTCCATATGTAc                        >  1:672635/1‑140 (MQ=255)                                                                                                           ttCTGCGCTTTTTAGTCCAGCGGTGTGTTAATCGGCAGCTCCCCCAAAGTTAAGGTGGGGGAGATAGATTAGTTGTACATTACCACGATTTTGACTCGGCTCATTATTTGCCCGCTTGAGACATTGTTTCCATATGTAcg                       <  2:479687/140‑1 (MQ=255)                                                                                                            tCTGCGCTTTTTAGTCCAGCGGTGTGTTAATCGGCAGCTCCCCCAAAGTTAAGGTGGGGGAGATAGATTAGTTGTACATTACCACGATTTTGACTCGGCTCATTATTTGCCCGCTTGAGACATTGTTTCCATATGTAcgc                      >  2:387306/1‑140 (MQ=255)                                                                                                            tCTGCGCTTTTTAGTCCAGCGGTGTGTTAATCGGCAGCTCCCCCAAAGTTAAGGTGGGGGAGATAGATTAGTTGTACATTACCACGATTTTGACTCGGCTCATTATTTGCCCGCTTGAGACATTGTTTCCATATGTAcgc                      >  1:403755/1‑140 (MQ=255)                                                                                                                          tCCAGCGGTGTGTTAATCGGCAGCTCCCCCAAAGTTAAGGTGGGGGAGATAGATTAGTTGTACATTACCACGATTTTGACTCGGCTCATTATTTGCCCGCTTGAGACATTGTTTCCATATGTACGCGGGCGAATAAATag        <  2:672635/140‑1 (MQ=255)                                                                                                                           ccAGCGGTGTGTTAATCGGCAGCTCCCCCAAAGTTAAGGTGGGGGAGATAGATTAGTTGTACATTACCACGATTTTGACTCGGCTCATTATTTGCCCGCTTGAGACATTGTTTCCATATGTACGCGGGCGAATAAATaga       <  1:213832/140‑1 (MQ=255)                                                                                                                             aGCGGTGTGTTAATCGGCAGCTCCCCCAAAGTTAAGGTGGGGGAGATAGATTAGTTGTACATTACCACGATTTTGACTCGGCTCATTATTTGCCCGCTTGAGACATTGTTTCCATATGTACGCGGGCGAATAAATAGAgg     <  2:750432/140‑1 (MQ=255)                                                                                                                               cGGTGTGTTAATCGGCAGCTCCCCCAAAGTTAAGGTGGGGGAGATAGATTAGTTGTACATTACCACGATTTTGACTCGGCTCATTATTTGCCCGCTTGAGACATTGTTTCCATATGTACGCGGGCGa                <  2:701347/127‑1 (MQ=255)                                                                                                                               cGGTGTGTTAATCGGCAGCTCCCCCAAAGTTAAGGTGGGGGAGATAGATTAGTTGTACATTACCACGATTTTGACTCGGCTCATTATTTGCCCGCTTGAGACATTGTTTCCATATGTACGCGGGCGa                >  1:701347/1‑127 (MQ=255)                                                                                                                               cGGTGTGTTAATCGGCAGCTCCCCCAAAGTTAAGGTGGGGGAGATAGATTAGTTGTACATTACCACGATTTTGACTCGGCTCATTATTTGCCCGCTTGAGACATTGTTTCCATATGTACGCGGGCGAATAAATaga       >  2:787343/1‑136 (MQ=255)                                                                                                                               cGGTGTGTTAATCGGCAGCTCCCCCAAAGTTAAGGTGGGGGAGATAGATTAGTTGTACATTACCACGATTTTGACTCGGCTCATTATTTGCCCGCTTGAGACATTGTTTCCATATGTACGCGGGCGAATAAATaga       <  1:787343/136‑1 (MQ=255)                                                                                                                               cGGTGTGTTAATCGGCAGCTCCCCCAAAGTTAAGGTGGGGGAGATAGATTAGTTGTACATTACCACGATTTTGACTCGGCTCATTATTTGCCCGCTTGAGACATTGTTTCCATATGTACGCGGGCGAATAAATAGAGGaa   <  1:713819/140‑1 (MQ=255)                                                                                                                               cGGTGTGTTAATCGGCAGCTCCCCCAAAGTTAAGGTGGGGGAGATAGATTAGTTGTACATTACCACGATTTTGACTCGGCTCATTATTTGCCCGCTTGAGACATTGTTTCCATATGTACGCGGGCGAATAAATAGAGGaa   >  1:279204/1‑140 (MQ=255)                                                                                                                               cGGTGTGTTAATCGGCAGCTCCCCCAAAGTTAAGGTGGGGGAGATAGATTAGTTGTACATTACCACGATTTTGACTCGGCTCATTATTTGCCCGCTTGAGACATTGTTTCCATATGTACGCGGGCGAATAAATAGAGGaa   >  2:859465/1‑140 (MQ=255)                                                                                                                                ggTGTGTTAATCGGCAGCTCCCCCAAAGTTAAGGTGGGGGAGATAGATTAGTTGTACATTACCACGATTTTGACTCGGCTCATTATTTGCCCGCTTGAGACATTGTTTCCATATGTACGCGGGCGAATAAATAGAGGAAt  <  1:68646/140‑1 (MQ=255)                                                                                                                                    |                                                                                                                                        TCGTAGAAATTGGTCGCGTCGAGAATGTGATGGAAGAGTACCATGCTATTTTTGCTGAGCGGATGATTCAGCACCCGGCGGTACAGCGAATCTGCAATACGGATTATTCTGCGCTTTTTAGTCCAGCGGTGCGTTAATCGGCAGCTCCCCCAAAGTTAAGGTGGGGGAGATAGATTAGTTGTACATTACCACGATTTTGACTCGGCTCATTATTTGCCCGCTTGAGACATTGTTTCCATATGTACGCGGGCGAATAAATAGAGGAAT  >  NC_000913/19484‑19750

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑