Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	414,639	A→G	F372S (TTC→TCC)	sbcC ←	ATP dependent, structure specific DNA nuclease ‑ SbcC subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	414,639	0	A	G	100.0%	96.0 / NA	29	F372S (TTC→TCC)	sbcC	ATP dependent, structure specific DNA nuclease ‑ SbcC subunit
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (15/14);  total (15/14)

CGCCAGGGCGGTAGCAACTTCATCGGCGGTTAACGTCAACGTGATCGCCGCAAGCGCATTAAGTTTTTGCTCAGCATGGGTTAACTGTTGCTGCCATTGCCGCAGATGCTCGCGATCGCTGGTTTGTTGGGAGAACTGCGCACGCCAACCCGCCGGTTCGTTGTTCCACTGACGGAAGCGGTCGTGTTCCTGTAACCAGGTATTCAGGCTTTGTTGCTGCTGCTGTAATTCTGCTGACTGCTTCGCCGCGTGGTGGCGAATGCTCGCGCG  >  NC_000913/414506‑414775                                                                                                                                      |                                                                                                                                         cgcCAGGGCGGTAGCAACTTCATCGGCGGTTAACGTCAACGTGATCGCCGCAAGCGCATTAAGTTTTTGCTCAGCATGGGTTAACTGTTGCTGCCATTGCCGCAGATGCTCGCGATCGCTGGTTTGTTGGGAGGACTgcg                                                                                                                                    <  2:554233/140‑1 (MQ=255)                   ttCATCGGCGGTTAACGTCAACGTGATCGCCGCAAGCGCATTAAGTTTTTGCTCAGCATGGGTTAACTGTTGCTGCCATTGCCGCAGATGCTCGCGATCGCTGGTTTGTTGGGAGGACTGCGCACGCCAACCCGCCGGtt                                                                                                                  <  2:817850/140‑1 (MQ=255)                   ttCATCGGCGGTTAACGTCAACGTGATCGCCGCAAGCGCATTAAGTTTTTGCTCAGCATGGGTTAACTGTTGCTGCCATTGCCGCAGATGCTCGCGATCGCTGGTTTGTTGGGAGGACGGCGCACGCCAACCCGCCGGtt                                                                                                                  <  1:135314/140‑1 (MQ=255)                                       aCGTGATCGCCGCAAGCGCATTAAGTTTTTGCTCAGCATGGGTTAACTGTTGCTGCCATTGCCGCAGATGCTCGCGATCGCTGGTTTGTTGGGAGGACTGCGCACGCCAACCCGCCGGTTCGTTGTTCCACTGACGGAAg                                                                                              <  1:600089/140‑1 (MQ=255)                                        cGTGATCGCCGCAAGCGCATTAAGTTTTTGCTCAGCATGGGTTAACTGTTGCTGCCATTGCCGCAGATGCTCGCGATCGCTGGTTTGTTGGGAGGACTGCGCACGCCAACCCGCCGGTTCGTTGTTCCACTGACGGAAGc                                                                                             >  1:814430/1‑140 (MQ=255)                                        cGTGATCGCCGCAAGCGCATTAAGTTTTTGCTCAGCATGGGTTAACTGTTGCTGCCATTGCCGCAGATGCTCGCGATCGCTGGTTTGTTGGGAGGACTGCGCACGCCAACCCGCCGGTTCGTTGTTCCACTGACGGAAGc                                                                                             >  2:123682/1‑140 (MQ=255)                                          tGATCGCCGCAAGCGCATTAAGTTTTTGCTCAGCATGGGTTAACTGTTGCTGCCATTGCCGCAGATGCTCGCGATCGCTGGTTTGTTGGGAGGACTGCGCACGCCAACCCGCCGGTTCGTTGTTCCACTGACGGAAGCgg                                                                                           <  1:208485/140‑1 (MQ=255)                                            aTCGCCGCAAGCGCATTAAGTTTTTGCTCAGCATGGGTTAACTGTTGCTGCCATTGCCGCAGATGCTCGCGATCGCTGGTTTGTTGGGAGGACTGCGCACGCCAACCCGCCGGTTCGTTGTTCCACTGACGGAAGCGGTc                                                                                         >  1:557630/1‑140 (MQ=255)                                               gccgcAAGCGCATTAAGTTTTTGCTCAGCATGGGTTAACTGTTGCTGCCATTGCCGCAGATGCTCGCGATCGCTGGTTTGTTGGGAGGACTGCGCACGCCAACCCGCCGGTTCGTTGTTCCACTGACGGAAGCGGTCgtg                                                                                      >  1:507479/1‑140 (MQ=255)                                                    aaGCGCTTTAGGTTTTGGCTCAGGAGGGTTAAACTGTGGCTGCCATTGCCGCAGATGCTCGCGACCGTTGGTTTTTTGGGAGGCCTGCGCACGCAAACCCGCCGGTTCGTTGTTCCACTGACGGAAGCGGTCGTGTTCCt                                                                                 <  2:557630/140‑1 (MQ=255)                                                     aGCGCATTAAGTTTTTGCTCAGCATGGGTTAACTGTTGCTGCCATTGCCGCAGATGCTCGCGATCGCTGGTTTGTTGGGAGGACTGCGCACGCCAACCCGCCGGTTCGTTGTTCCACTGACGGAAGCGGTCGTGTTCCTg                                                                                <  1:310839/140‑1 (MQ=255)                                                     aGCGCATTAAGTTTTTGCTCAGCATGGGTTAACTGTTGCTGCCATTGCCGCAGATGCTCGCGATCGCTGGTTTGTTGGGAGGACTGCGCACGCCAACCCGCCGGTTCGTTGTTCCACTGACGGAAGCGGTCGTGTTCCTg                                                                                <  1:300028/140‑1 (MQ=255)                                                          aTTAAGTTTTTGCTCAGCATGGGTTAACTGTTGCTGCCATTGCCGCAGATGCTCGCGATCGCTGGTTTGTTGGGAGGACTGCGCACGCCAACCCGCCGGTTCGTTGTTCCACTGACGGAAGCGGTCGTGTTCCTGTAAcc                                                                           >  1:323613/1‑140 (MQ=255)                                                           ttAAGTTTTTGCTCAGCATGGGTTAACTGTTGCTGCCATTGCCGCAGATGCTCGCGATCGCTGGTTTGTTGGGAGGACTGCGCACGCCAACCCGCCGGTTCGTTGTTCCACTGACGGAAGCGGTCGTGTTCCTGTAACCa                                                                          >  2:34338/1‑140 (MQ=255)                                                                           cATGGGTTAACTGTTGCTGCCATTGCCGCAGATGCTCGCGATCGCTGGTTTGTTGGGAGGACTGCGCACGCCAACCCGCCAGTTCGTTGTTCCACTGACGGAAGCGGTCGTGTTCCTGTAACCAGGTATTCAGGCTttgt                                                          <  2:814430/140‑1 (MQ=255)                                                                                     cTGTTGCTGCCATTGCCGCAGATGCTCGCGATCGCTGGTTTGTTGGGAGGACTGCGCACGCCAACCCGCCGGTTCGTTGTTCCACTGACGGAAGCGGTCGTGTTCCTGTAACCAGGTATTCAGGCTTTGTTgctgctgct                                                >  2:477457/1‑140 (MQ=255)                                                                                     cTGTTGCTGCCATTGCCGCAGATGCTCGCGATCGCTGGTTTGTTGGGAGGACTGCGCACGCCAACCCGCCGGTTCGTTGTTCCACTGACGGAAGCGGTCGTGTTCCTGTAACCAGGTATTCAGGCTTTGTTgctgctgct                                                >  2:154236/1‑140 (MQ=255)                                                                                         tgctgcCATTGCCGCAGATGCTCGCGATCGCTGGTTTGTTGGGAGGACTGCGCACGCCAACCCGCCGGTTCGTTGTTCCACTGACGGAAGCGGTCGTGTTCCTGTAACCAGGTATTCAGGCTTTGTTGCTGCTGCTGTaa                                            >  1:34441/1‑140 (MQ=255)                                                                                         tgctgcCATTGCCGCAGATGCTCGCGATCGCTGGTTTGTTGGGAGGACTGCGCACGCCAACCCGCCGGTTCGTTGTTCCACTGACGGAAGCGGTCGTGTTCCTGTAACCAGGTATTCAGGCTTTGTTGCTGCTGCTGTaa                                            >  2:564041/1‑140 (MQ=255)                                                                                         tgctgcCATTGCCGCAGATGCTCGCGATCGCTGGTTTGTTGGGAGGACTGCGCACGCCAACCCGCCGGTTCGTTGTTCCACTGACGGAAGCGGTCGTGTTCCTGTAACCAGGTATTCAGGCTTTGTTGCTGCTGCTGTaa                                            <  2:471894/140‑1 (MQ=255)                                                                                          gctgcCATTGCCGCAGATGCTCGCGATCGCTGGTTTGTTGGGAGGACTGCGCACGCCAACCCGCCGGTTCGTTGTTCCACTGACGGAAGCGGTCGTGTTCCTGTAACCAGGTATTCAGGCTTTGTTGCTGCTGCTGTaa                                            <  1:910644/139‑1 (MQ=255)                                                                                          gctgcCATTGCCGCAGATGCTCGCGATCGCTGGTTTGTTGGGAGGACTGCGCACGCCAACCCGCCGGTTCGTTGTTCCACTGACGGAAGCGGTCGTGTTCCTGTAACCAGGTATTCAGGCTTTGTTGCTGCTGCTGTaa                                            >  2:910644/1‑139 (MQ=255)                                                                                          gctgcCATTGCCGCAGATGCTCGCGATCGCTGGTTTGTTGGGAGGACTGCGCACGCCAACCCGCCGGTTCGTTGTTCCACTGACGGAAGCGGTCGTGTTCCTGTAACCAGGTATTCAGGCTTTGTTGCTGCTGCTGTAAt                                           >  1:808354/1‑140 (MQ=255)                                                                                                tttGCCGCAGATGCTCGCGATCGCTGGTTTGTTGGGAGGACTGCGCACGCCAACCCGCCGGTTCGTTGTTCCACTGACGGAAGCGGTCGTGTTCCTGTAACCAGGTATTCAGGCTTTGTTGCTGCTGCTGTAATTctgct                                     <  2:748434/139‑1 (MQ=255)                                                                                                        aGATGCTCGCGATCGCTGGTTTGTTGGGAGGACTGCGCACGCCAACCCGCCGGTTCGTTGTTCCACTGACGGAAGCGGTCGTGTTCCTGTAACCAGGTATTCAGGCTTTGTTGCTGCTGCTGTAATTCTGCTGACTGCtt                             <  1:123682/140‑1 (MQ=255)                                                                                                             cTCGCGATCGCTGGTTTGTTGGGAGGACTGCGCACGCCAACCCGCCGGTTCGTTGTTCCACTGACGGAAGCGGTCGTGTTCCTGTAACCAGGTATTCAGGCTTTGTTGCTGCTGCTGTAATTCTGCTGACTGCTTcgccg                        >  1:532324/1‑140 (MQ=255)                                                                                                              tCGCGATCGCTGGTTTGTTGGGAGGACTGCGCACGCCAACCCGCCGGTTCGTTGTTCCACTGACGGAAGCGGTCGTGTTCCTGTAACCAGGTATTCAGGCTTTGTTGCTGCTGCTGTAATTCTGCTGACTGCTTcgccgc                       >  1:599188/1‑140 (MQ=255)                                                                                                               cgcgATCGCTGGTTTGTTGGGAGGACTGCGCACGCCAACCCGCCGGTTCGTTGTTCCACTGACGGAAGCGGTCGTGTTCCTGTAACCAGGTATTCAGGCTTTGTTGCTGCTGCTGTAATTCTGCTGACTGCTTCGCcgcg                      <  1:6105/140‑1 (MQ=255)                                                                                                               cgcgATCGCTGGTTTGTTGGGAGGACTGCGCACGCCAACCCGCCGGTTCGTTGTTCCACTGACGGAAGCGGTCGTGTTCCTGTAACCAGGTATTCAGGCTTTGTTGCTGCTGCTGTAATTCTGCTGACTGCTTCGCcgcg                      <  1:154236/140‑1 (MQ=255)                                                                                                                            ttgttgGGAGGACTGCGCACGCCAACCCGCCGGTTCGTTGTTCCACTGACGGAAGCGGTCGTGTTCCTGTAACCAGGTATTCAGGCTTTGTTGCTGCTGCTGTAATTCTGCTGACTGCTTCGCCGCGTGGTGGCGAATGc         >  2:66913/1‑140 (MQ=255)                                                                                                                                   gaggACTGCGCACGCCAACCCGCCGGTTCGTTGTTCCACTGACGGAAGCGGTCGTGTTCCTGTAACCAGGTATTCAGGCTTTGTTGCTGCTGCTGTAATTCTGCTGACTGCTTCGCCGCGTGGTGGCGAATGCTcgcgcg  <  2:599188/140‑1 (MQ=255)                                                                                                                                      |                                                                                                                                         CGCCAGGGCGGTAGCAACTTCATCGGCGGTTAACGTCAACGTGATCGCCGCAAGCGCATTAAGTTTTTGCTCAGCATGGGTTAACTGTTGCTGCCATTGCCGCAGATGCTCGCGATCGCTGGTTTGTTGGGAGAACTGCGCACGCCAACCCGCCGGTTCGTTGTTCCACTGACGGAAGCGGTCGTGTTCCTGTAACCAGGTATTCAGGCTTTGTTGCTGCTGCTGTAATTCTGCTGACTGCTTCGCCGCGTGGTGGCGAATGCTCGCGCG  >  NC_000913/414506‑414775

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑