Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,347,218	T→C	V35A (GTG→GCG)	rapZ →	RNase adaptor protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,347,218	0	T	C	100.0%	162.2 / NA	45	V35A (GTG→GCG)	rapZ	RNase adaptor protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (26/19);  total (26/19)

ACGTTGTGAGGAGAAACAGTACATGGTACTGATGATCGTCAGCGGACGTTCAGGTTCAGGTAAATCTGTCGCCCTGCGTGCGCTGGAAGATATGGGTTTTTACTGCGTGGATAACCTTCCCGTAGTGTTGTTACCCGATCTGGCTCGAACCCTGGCCGATCGCGAGATTTCTGCCGCCGTCAGCATTGATGTTCGTAATATGCCGGAGTCACCAGAAATATTCGAACAGGCGATGAGTAACCTGCCTGACGCTTTCTCAC  >  NC_000913/3347093‑3347352                                                                                                                              |                                                                                                                                       aCGTTGTGAGGAGAAACAGTACATGGTACTGATGATCGTCAGCGGACGTTCAGGTTCAGGTAAATCTGTCGCCCTGCGTGCGCTGGAAGATATGGGTTTTTACTGCGTGGATAACCTTCCCGTAGCGTTGTTACCCGAt                                                                                                                           <  1:193017/139‑1 (MQ=255) aCGTTGTGAGGAGAAACAGTACATGGTACTGATGATCGTCAGCGGACGTTCAGGTTCAGGTAAATCTGTCGCCCTGCGTGCGCTGGAAGATATGGGTTTTTACTGCGTGGATAACCTTCCCGTAGCGTTGTTACCCGAt                                                                                                                           >  2:193017/1‑139 (MQ=255)      gtgAGGAGAAACAGTACATGGTACTGATGATCGTCAGCGGACGTTCAGGTTCAGGTAAATCTGTCGCCCTGCGTGCGCTGGAAGATATGGGTTTTTACTGCGTGGATAACCTTCCCGTAGCGTTGTTACCCGATCTGGCt                                                                                                                     <  1:281346/140‑1 (MQ=255)             gaAACAGTACATGGTACTGATGATCGTCAGCGGACGTTCAGGTTCAGGTAAATCTGTCGCCCTGCGTGCGCTGGAAGATATGGGTTTTTACTGCGTGGATAACCTTCCCGTAGCGTTGTTACCCGATCTGGCTCGAAccc                                                                                                              >  1:203982/1‑140 (MQ=255)                              tgatgaTCGTCAGCGGACGTTCAGGTTCAGGTAAATCTGTCGCCCTGCGTGCGCTGGAAGATATGGGTTTTTACTGCGTGGATAACCTTCCCGTAGCGTTGTTACCCGATCTGGCTCGAACCCTGGCCGATCGCGAGAtt                                                                                             >  1:248058/1‑140 (MQ=255)                              tgatgaTCGTCAGCGGACGTTCAGGTTCAGGTAAATCTGTCGCCCTGCGTGCGCTGGAAGATATGGGTTTTTACTGCGTGGATAACCTTCCCGTAGCGTTGTTACCCGATCTGGCTCGAACCCTGGCCGATCGCGAGAtt                                                                                             >  2:152705/1‑140 (MQ=255)                                  gatCGTCAGCGGACGTTCAGGTTCAGGTAAATCTGTCGCCCTGCGTGCGCTGGAAGATATGGGTTTTTACTGCGTGGATAACCTTCCCGTAGCGTTGTTACCCGATCTGGCTCGAACCCTGGCCGATCGCGAGATTTCTg                                                                                         <  2:203982/140‑1 (MQ=255)                                   atCGTCAGCGGACGTTCAGGTTCAGGTAAATCTGTCGCCCTGCGTGCGCTGGAAGATATGGGTTTTTACTGCGTGGATAACCTTCCCGTAGCGTTGTTACCCGATCTGGCTCGAACCCTGGCCGATCGCGAGATTTCTgc                                                                                        >  2:176993/1‑140 (MQ=255)                                     cGTCAGCGGACGTTCAGGTTCAGGTAAATCTGTCGCCCTGCGTGCGCTGGAAGATATGGGTTTTTACTGCGTGGATAACCTTCCCGTAGCGTTGTTACCCGATCTGGCTCGAACCCTGGCCGATCGCGAGATTTCTgccg                                                                                      >  2:965615/1‑140 (MQ=255)                                     cGTCAGCGGACGTTCAGGTTCAGGTAAATCTGTCGCCCTGCGTGCGCTGGAAGATATGGGTTTTTACTGCGTGGATAACCTTCCCGTAGCGTTGTTACCCGATCTGGCTCGAACCCTGGCCGATCGCGAGATTTCTgccg                                                                                      >  2:482473/1‑140 (MQ=255)                                     cGTCAGCGGACGTTCAGGTTCAGGTAAATCTGTCGCCCTGCGTGCGCTGGAAGATATGGGTTTTTACTGCGTGGATAACCTTCCCGTAGCGTTGTTACCCGATCTGGCTCGAACCCTGGCCGATCGCGAGATTTCTgccg                                                                                      >  1:198952/1‑140 (MQ=255)                                     cGTCAGCGGACGTTCAGGTTCAGGTAAATCTGTCGCCCTGCGTGCGCTGGAAGATATGGGTTTTTACTGCGTGGATAACCTTCCCGTAGCGTTGTTACCCGATCTGGCTCGAACCCTGGCCGATCGCGAGATTTCTgccg                                                                                      >  1:329528/1‑140 (MQ=255)                                     cGTCAGCGGACGTTCAGGTTCAGGTAAATCTGTCGCCCTGCGTGCGCTGGAAGATATGGGTTTTTACTGCGTGGATAACCTTCCCGTAGCGTTGTTACCCGATCTGGCTCGAACCCTGGCCGATCGCGAGATTTCTgccg                                                                                      >  1:43190/1‑140 (MQ=255)                                     cGTCAGCGGACGTTCAGGTTCAGGTAAATCTGTCGCCCTGCGTGCGCTGGAAGATATGGGTTTTTACTGCGTGGATAACCTTCCCGTAGCGTTGTTACCCGATCTGGCTCGAACCCTGGCCGATCGCGAGATTTCTgc                                                                                        >  1:17092/1‑138 (MQ=255)                                     cGTCAGCGGACGTTCAGGTTCAGGTAAATCTGTCGCCCTGCGTGCGCTGGAAGATATGGGTTTTTACTGCGTGGATAACCTTCCCGTAGCGTTGTTACCCGATCTGGCTCGAACCCTGGCCGATCGCGAGATTTCTgc                                                                                        <  2:17092/138‑1 (MQ=255)                                       tCAGCGGACGTTCAGGTTCAGGTAAATCTGTCGCCCTGCGTGCGCTGGAAGATATGGGTTTTTACTGCGTGGATAACCTTCCCGTAGCGTTGTTACCCGATCTGGCTCGAACCCTGGCCGATcgcg                                                                                                  >  2:769329/1‑126 (MQ=255)                                       tCAGCGGACGTTCAGGTTCAGGTAAATCTGTCGCCCTGCGTGCGCTGGAAGATATGGGTTTTTACTGCGTGGATAACCTTCCCGTAGCGTTGTTACCCGATCTGGCTCGAACCCTGGCCGATcgcg                                                                                                  <  1:769329/126‑1 (MQ=255)                                       tCAGCGGACGTTCAGGTTCAGGTAAATCTGTCGCCCTGCGTGCGCTGGAAGATATGGGTTTTTACTGCGTGGATAACCTTCCCGTAGCGTTGTTACCCGATCTGGCTCGAACCCTGGCCGATCGCGAGATTTCTgccgcc                                                                                    <  1:894320/140‑1 (MQ=255)                                        cAGCGGACGTTCAGGTTCAGGTAAATCTGTCGCCCTGCGTGCGCTGGAAGATATGGGTTTTTACTGCGTGGATAACCTTCCCGTAGCGTTGTTACCCGATCTGGCTCGAACCCTGGCCGATCGCGAGATTTCTGccgccg                                                                                   <  1:664854/140‑1 (MQ=255)                                         aGCGGACGTTCAGGTTCAGGTAAATCTGTCGCCCTGCGTGCGCTGGAAGATATGGGTTTTTACTGCGTGGATAACCTTCCCGTAGCGTTGTTACCCGATCTGGCTCGAACCCTGGCCGATCGCGAGATTTCTGCCGCCGt                                                                                  <  1:965615/140‑1 (MQ=255)                                         aGCGGACGTTCAGGTTCAGGTAAATCTGTCGCCCTGCGTGCGCTGGAAGATATGGGTTTTTACTGCGTGGATAACCTTCCCGTAGCGTTGTTACCCGATCTGGCTCGAACCCTGGCCGATCGCGAGATTTCTGCCGCCGt                                                                                  <  1:482473/140‑1 (MQ=255)                                           cGGACGTTCAGGTTCAGGTAAATCTGTCGCCCTGCGTGCGCTGGAAGATATGGGTTTTTACTGCGTGGATAACCTTCCCGTAGCGTTGTTACCCGATCTGGCTCGAACCCTGGCCGATCGCGAGATTTCTGCCGCCGTCa                                                                                <  2:688200/140‑1 (MQ=255)                                               cGTTCAGGTTCAGGTAAATCTGTCGCCCTGCGTGCGCTGGAAGATATGGGTTTTTACTGCGTGGATAACCTTCCCGTAGCGTTGTTACCCGATCTGGCTCGAACCCTGGCCGATCGCGAGATTTCTGCCGCCGTCAGCAt                                                                            >  2:127024/1‑140 (MQ=255)                                                   caggttcaggtAAATCTGTCGCCCTGCGTGCGCTGGAAGATATGGGTTTTTACTGCGTGGATAACCTTCCCGTAGCGTTGTTACCCGATCTGGCTCGAACCCTGGCCGATCGCGAGATTTCTGCCGCCGTCAGCATTGAt                                                                        <  1:176993/140‑1 (MQ=255)                                                          aggtAAATCTGTCGCCCTGCGTGCGCTGGAAGATATGGGTTTTTACTGCGTGGATAACCTTCCCGTAGCGTTGTTACCCGATCTGGCTCGAACCCTGGCCGATCGCGAGATTTCTGCCGCCGTCAGCATTGATGTTCGTa                                                                 <  1:127024/140‑1 (MQ=255)                                                                         ccTGCGTGCGCTGGAAGATATGGGTTTTTACTGCGTGGATAACCTTCCCGTAGCGTTGTTACCCGATCTGGCTCGAACCCTGGCCGATCGCGAGATTTCTGCCGCCGTCAGCATTGATGTTCGTAATATGCCGGAGTCAc                                                  >  1:450325/1‑140 (MQ=255)                                                                                       aaGATATGGGTTTTTACTGCGTGGATAACCTTCCCGTAGCGTTGTTACCCGATCTGGCTCGAACCCTGGCCGATCGCGAGATTTCTGCCGCCGTCAGCATTGATGTTCGTAATATGCCGGAGTCACCAGAAATATTCGaa                                    >  1:965987/1‑140 (MQ=255)                                                                                         gATATGGGTTTTTACTGCGTGGATAACCTTCCCGTAGCGTTGTTACCCGATCTGGCTCGAACCCTGGCCGATCGCGAGATTTCTGCCGCCGTCAGCATTGATGTTCGTAATATGCCGGAGTCACCAGAAATATTCGAACa                                  <  2:43190/140‑1 (MQ=255)                                                                                           tatGGGTTTTTACTGCGTGGATAACCTTCCCGTAGCGTTGTTACCCGATCTGGCTCGAACCCTGGCCGATCGCGAGATTTCTGCCGCCGTCAGCATTGATGTTCGTAATATGCCGGAGTCACCAGAAATATTCGAACAgg                                <  2:383552/140‑1 (MQ=255)                                                                                           tatGGGTTTTTACTGCGTGGATAACCTTCCCGTAGCGTTGTTACCCGATCTGGCTCGAACCCTGGCCGATCGCGAGATTTCTGCCGCCGTCAGCATTGATGTTCGTAATATGCCGGAGTCACCAGAAATATTCGAACAgg                                >  2:622932/1‑140 (MQ=255)                                                                                            atGGGTTTTTACTGCGTGGATAACCTTCCCGTAGCGTTGTTACCCGATCTGGCTCGAACCCTGGCCGATCGCGAGATTTCTGCCGCCGTCAGCATTGATGTTCGTAATATGCCGGAGTCACCAGAAATATTCGAACAGGc                               <  2:198952/140‑1 (MQ=255)                                                                                                gTTTTTACTGCGTGGATAACCTTCCCGTAGCGTTGTTACCCGATCTGGCTCGAACCCTGGCCGATCGCGAGATTTCTGCCGCCGTCAGCATTGATGTTCGTAATATGCCGGAGTCACCAGAAATATTCGAACAGGCGATg                           <  2:248058/140‑1 (MQ=255)                                                                                                gTTTTTACTGCGTGGATAACCTTCCCGTAGCGTTGTTACCCGATCTGGCTCGAACCCTGGCCGATCGCGAGATTTCTGCCGCCGTCAGCATTGATGTTCGTAATATGCCGGAGTCACCAGAAATATTCGAACAGGCGATg                           >  1:735430/1‑140 (MQ=255)                                                                                                          cGTGGATAACCTTCCCGTAGCGTTGTTACCCGATCTGGCTCGAACCCTGGCCGATCGCGAGATTTCTGCCGCCGTCAGCATTGATGTTCGTAATATGCCGGAGTCACCAGAAATATTCGAACAGGCGATGAGTAAcctgc                 >  2:228917/1‑140 (MQ=255)                                                                                                          cGTGGATAACCTTCCCGTAGCGTTGTTACCCGATCTGGCTCGAACCCTGGCCGATCGCGAGATTTCTGCCGCCGTCAGCATTGATGTTCGTAATATGCCGGAGTCACCAGAAATATTCGAACAGGCGATGAGTAAcctgc                 >  2:294583/1‑140 (MQ=255)                                                                                                          cGTGGATAACCTTCCCGTAGCGTTGTTACCCGATCTGGCTCGAACCCTGGCCGATCGCGAGATTTCTGCCGCCGTCAGCATTGATGTTCGTAATATGCCGGAGTCACCAGAAATATTCGAACAGGCGATGAGTAAcctgc                 >  2:472948/1‑140 (MQ=255)                                                                                                              gATAACCTTCCCGTAGCGTTGTTACCCGATCTGGCTCGAACCCTGGCCGATCGCGAGATTTCTGCCGCCGTCAGCATTGATGTTCGTAATATGCCGGAGTCACCAGAAATATTCGAACAGGCGATGAGTAACCTGCCTGa             >  1:503649/1‑140 (MQ=255)                                                                                                              gATAACCTTCCCGTAGCGTTGTTACCCGATCTGGCTCGAACCCTGGCCGATCGCGAGATTTCTGCCGCCGTCAGCATTGATGTTCGTAATATGCCGGAGTCACCAGAAATATTCGAACAGGCGATGAGTAACCTGCCTGa             <  2:735430/140‑1 (MQ=255)                                                                                                                    cTTCCCGTAGCGTTGTTACCCGATCTGGCTCGAACCCTGGCCGATCGCGAGATTTCTGCCGCCGTCAGCATTGATGTTCGTAATATGCCGGAGTCACCAGAAATATTCGAACAGGCGATGAGTAAcctgc                 >  1:309136/1‑130 (MQ=255)                                                                                                                    cTTCCCGTAGCGTTGTTACCCGATCTGGCTCGAACCCTGGCCGATCGCGAGATTTCTGCCGCCGTCAGCATTGATGTTCGTAATATGCCGGAGTCACCAGAAATATTCGAACAGGCGATGAGTAAcctgc                 <  2:309136/130‑1 (MQ=255)                                                                                                                      tCCCGTAGCGTTGTTACCCGATCTGGCTCGAACCCTGGCCGATCGCGAGATTTCTGCCGCCGTCAGCATTGATGTTCGTAATATGCCGGAGTCACCAGAAATATTCGAACAGGCGATGAGTAACCTGCCTGACGCTTtct     >  1:604097/1‑140 (MQ=255)                                                                                                                      tCCCGTAGCGTTGTTACCCGATCTGGCTCGAACCCTGGCCGATCGCGAGATTTCTGCCGCCGTCAGCATTGATGTTCGTAATATGCCGGAGTCACCAGAAATATTCGAACAGGCGATGAGTAACCTGCCTGACGCTTtct     >  1:784885/1‑140 (MQ=255)                                                                                                                       cccGTAGCGTTGTTACCCGATCTGGCTCGAACCCTGGCCGATCGCGAGATTTCTGCCGCCGTCAGCATTGATGTTCGTAATATGCCGGAGTCACCAGAAATATTCGAACAGGCGATGAGTAACCTGCCTGACGCTTtctc    <  2:604097/140‑1 (MQ=255)                                                                                                                       cccGTAGCGTTGTTACCCGATCTGGCTCGAACCCTGGCCGATCGCGAGATTTCTGCCGCCGTCAGCATTGATGTTCGTAATATGCCGGAGTCACCAGAAATATTCGAACAGGCGATGAGTAACCTGCCTGACGCTTtctc    >  2:252491/1‑140 (MQ=255)                                                                                                                         cGTAGCGTTGTTACCCGATCTGGCTCGAACCCTGGCCGATCGCGAGATTTCTGCCGCCGTCAGCATTGATGTTCGTAATATGCCGGAGTCACCAGAAATATTCGAACAGGCGATGAGTAACCTGCCTGACGCTTTCTCAc  >  1:512874/1‑140 (MQ=255)                                                                                                                              |                                                                                                                                       ACGTTGTGAGGAGAAACAGTACATGGTACTGATGATCGTCAGCGGACGTTCAGGTTCAGGTAAATCTGTCGCCCTGCGTGCGCTGGAAGATATGGGTTTTTACTGCGTGGATAACCTTCCCGTAGTGTTGTTACCCGATCTGGCTCGAACCCTGGCCGATCGCGAGATTTCTGCCGCCGTCAGCATTGATGTTCGTAATATGCCGGAGTCACCAGAAATATTCGAACAGGCGATGAGTAACCTGCCTGACGCTTTCTCAC  >  NC_000913/3347093‑3347352

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑