Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,409,589	A→T	I174F (ATC→TTC)	prmA →	methyltransferase for 50S ribosomal subunit protein L11

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,409,589	0	A	T	100.0%	277.8 / NA	78	I174F (ATC→TTC)	prmA	methyltransferase for 50S ribosomal subunit protein L11
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (36/42);  total (36/42)

CGTGATGTTAGATCCAGGGCTGGCGTTTGGTACGGGTACCCATCCAACCACCTCTCTGTGCCTGCAATGGCTCGACAGCCTCGATTTAACCGGTAAAACAGTCATCGACTTTGGCTGTGGTTCCGGCATTCTGGCGATCGCGGCGCTGAAACTGGGTGCAGCAAAAGCCATTGGTATTGATATCGATCCGCAGGCGATTCAGGCCAGCCGCGATAACGCCGAACGTAATGGCGTTTCTGACCGTCTGGAACTCTACTTACCGAAAGATC  >  NC_000913/3409453‑3409721                                                                                                                                         |                                                                                                                                     cGTGATGTTAGATCCAGGGCTGGCGTTTGGTACGGGTACCCATCCAACCACCTCTCTGTGCCTGCAATGGCTCGACAGCCTCGATTTAACCGGTAAAACAGTCATCGACTTTGGCTGTGGTTCCGGCATTCTGGCGTTcg                                                                                                                                   <  2:641678/140‑1 (MQ=255)    gATGTTAGATCCAGGGCTGGCGTTTGGTACGGGTACCCATCCAACCACCTCTCTGTGCCTGCAATGGCTCGACAGCCTCGATTTAACCGGTAAAACAGTCATCGACTTTGGCTGTGGTTCCGGCATTCTGGCGTTCgcgg                                                                                                                                <  2:449192/140‑1 (MQ=255)      tGTTAGATCCAGGGCTGGCGTTTGGTACGGGTACCCATCCAACCACCTCTCTGTGCCTGCAATGGCTCGACAGCCTCGATTTAACCGGTAAAACAGTCATCGACTTTGGCTGTGGTTCCGGCATTCTGGCGTTCgcggcg                                                                                                                              <  1:451398/140‑1 (MQ=255)            aTCCAGGGCTGGCGTTTGGTACGGGTACCCATCCAACCACCTCTCTGTGCCTGCAATGGCTCGACAGCCTCGATTTAACCGGTAAAACAGTCATCGACTTTGGCTGTGGTTCCGGCATTCTGGCGTTCGCGGCGCTGaaa                                                                                                                        >  1:786830/1‑140 (MQ=255)              ccAGGGCTGGCGTTTGGTACGGGTACCCATCCAACCACCTCTCTGTGCCTGCAATGGCTCGACAGCCTCGATTTAACCGGTAAAACAGTCATCGACTTTGGCTGTGGTTCCGGCATTCTGGCGTTCGCGGCGCTGAAACt                                                                                                                      <  2:2312/140‑1 (MQ=255)               cAGGGCTGGCGTTTGGTACGGGTACCCATCCAACCACCTCTCTGTGCCTGCAATGGCTCGACAGCCTCGATTTAACCGGTAAAACAGTCATCGACTTTGGCTGTGGTTCCGGCATTCTGGCGTTCGCGGCGCTGAAACTg                                                                                                                     >  1:833917/1‑140 (MQ=255)                aGGGCTGGCGTTTGGTACGGGTACCCATCCAACCACCTCTCTGTGCCTGCAATGGCTCGACAGCCTCGATTTAACCGGTAAAACAGTCATCGACTTTGGCTGTGGTTCCGGCATTCTGGCGTTCGCGGCGCTGAAACTgg                                                                                                                    >  1:2085/1‑140 (MQ=255)                 gggCTGGCGTTTGGTACGGGTACCCATCCAACCACCTCTCTGTGCCTGCAATGGCTCGACAGCCTCGATTTAACCGGTAAAACAGTCATCGACTTTGGCTGTGGTTCCGGCATTCTGGCGTTCGCGGCGCTGAAACTggg                                                                                                                   >  2:252477/1‑140 (MQ=255)                  ggCTGGCGTTTGGTACGGGTACCCATCCAACCACCTCTCTGTGCCTGCAATGGCTCGACAGCCTCGATTTAACCGGTAAAACAGTCATCGACTTTGGCTGTGGTTCCGGCATTCTGGCGTTCGCGGCGCTGAAACTGGGt                                                                                                                  >  1:574653/1‑140 (MQ=255)                     tGGCGTTTGGTACGGGTACCCATCCAACCACCTCTCTGTGCCTGCAATGGCTCGACAGCCTCGATTTAACCGGTAAAACAGTCATCGACTTTGGCTGTGGTTCCGGCATTCTGGCGTTCGCGGCGCTGAAACTGGGTgca                                                                                                               >  1:61198/1‑140 (MQ=255)                     tGGCGTTTGGTACGGGTACCCATCCAACCACCTCTCTGTGCCTGCAATGGCTCGACAGCCTCGATTTAACCGGTAAAACAGTCATCGACTTTGGCTGTGGTTCCGGCATTCTGGCGTTCGCGGCGCTGAAACTGGGTgca                                                                                                               >  2:117171/1‑140 (MQ=255)                       gCGTTTGGTACGGGTACCCATCCAACCACCTCTCTGTGCCTGCAATGGCTCGACAGCCTCGATTTAACCGGTAAAACAGTCATCGACTTTGGCTGTGGTTCCGTCATTCTGGCGTTCGCGGCGCTGAAACTGGGTgcagc                                                                                                             >  1:70432/1‑140 (MQ=255)                       gCGTTTGGTACGGGTACCCATCCAACCACCTCTCTGTGCCTGCAATGGCTCGACAGCCTCGATTTAACCGGTAAAACAGTCATCGACTTTGGCTGTGGTTCCGGCATTCTGGCGTTCGCGGCGCTGAAACTGGGTgcagc                                                                                                             >  1:274114/1‑140 (MQ=255)                       gCGTTTGGTACGGGTACCCATCCAACCACCTCTCTGTGCCTGCAATGGCTCGACAGCCTCGATTTAACCGGTAAAACAGTCATCGACTTTGGCTGTGGTTCCGGCATTCTGGCGTTCGCGGCGCTGAAACTGGGTgcagc                                                                                                             >  1:633477/1‑140 (MQ=255)                       gCGTTTGGTACGGGTACCCATCCAACCACCTCTCTGTGCCTGCAATGGCTCGACAGCCTCGATTTAACCGGTAAAACAGTCATCGACTTTGGCTGTGGTTCCGGCATTCTGGCGTTCGCGGCGCTGAAACTGGGTgcagc                                                                                                             >  2:334902/1‑140 (MQ=255)                       gCGTTTGGTACGGGTACCCATCCAACCACCTCTCTGTGCCTGCAATGGCTCGACAGCCTCGATTTAACCGGTAAAACAGTCATCGACTTTGGCTGTGGTTCCGGCATTCTGGCGTTCGCGGCGCTGAAACTGGGTgcagc                                                                                                             >  1:306382/1‑140 (MQ=255)                         gTTTGGTACGGGTACCCATCCAACCACCTCTCTGTGCCTGCAATGGCTCGACAGCCTCGATTTAACCGGTAAAACAGTCATCGACTTTGGCTGTGGTTCCGGCATTCTGGCGTTCGCGGCGCTGAAACTGGGTGCAGCaa                                                                                                           <  1:252477/140‑1 (MQ=255)                         gTTTGGTACGGGTACCCATCCAACCACCTCTCTGTGCCTGCAATGGCTCGACAGCCTCGATTTAACCGGTAAAACAGTCATCGACTTTGGCTGTGGTTCCGGCATTCTGGCGTTCGCGGCGCTGAAACTGGGTGCAGCaa                                                                                                           <  1:679004/140‑1 (MQ=255)                         gTTTGGTACGGGTACCCATCCAACCACCTCTCTGTGCCTGCAATGGCTCGACAGCCTCGATTTAACCGGTAAAACAGTCATCGACTTTGGCTGTGGTTCCGACATTCTGGCGTTCGCGGCGCTGAAACTGGGTGCAGCaa                                                                                                           <  2:918830/140‑1 (MQ=255)                            tGGTACGGGTACCCATCCAACCACCTCTCTGTGCCTGCAATGGCTCGACAGCCTCGATTTAACCGGTAAAACAGTCATCGACTTTGGCTGTGGTTCCGGCATTCTGGCGTTCGCGGCGCTGAAACTGGGTGCAGCAAAAg                                                                                                        >  1:331717/1‑140 (MQ=255)                             ggTACGGGTACCCATCCAACCACCTCTCTGTGCCTGCAATGGCTCGACAGCCTCGATTTAACCGGTAAAACAGTCATCGACTTTGGCTGTGGTTCCGGCATTCTGGCGTTCGCGGCGCTGAAACTGGGTGCAGCAAAAGc                                                                                                       <  1:493216/140‑1 (MQ=255)                             ggTACGGGTACCCATCCAACCACCTCTCTGTGCCTGCAATGGCTCGACAGCCTCGATTTAACCGGTAAAACAGTCATCGACTTTGGCTGTGGTTCCGGCATTCTGGCGTTCGCGGCGCTGAAACTGGGTGCAGCAAAAGc                                                                                                       <  2:331717/140‑1 (MQ=255)                             ggTACGGGTACCCATCCAACCACCTCTCTGTGCCTGCAATGGCTCGACAGCCTCGATTTAACCGGTAAAACAGTCATCGACTTTGGCTGTGGTTCCGGCATTCTGGCGTTCGCGGCGCTGAAACTGGGTGCAGCAAAAGc                                                                                                       <  2:541540/140‑1 (MQ=255)                               tACGGGTACCCATCCAACCACCTCTCTGTGCCTGCAATGGCTCGACAGCCTCGATTTAACCGGTAAAACAGTCATCGACTTTGGCTGTGGTTCCGGCATTCTGGCGTTCGCGGCGCTGAAACTGGGTGCAGCAAAAGCCa                                                                                                     <  2:2085/140‑1 (MQ=255)                               tACGGGTACCCATCCAACCACCTCTCTGTGCCTGCAATGGCTCGACAGCCTCGATTTAACCGGTAAAACAGTCATCGACTTTGGCTGTGGTTCCGGCATTCTGGCGTTCGCGGCGCTGAAACTGGGTGCAGCAAAAGCCa                                                                                                     <  1:31321/140‑1 (MQ=255)                               tACGGGTACCCATCCAACCACCTCTCTGTGCCTGCAATGGCTCGACAGCCTCGATTTAACCGGTAAAACAGTCATCGACTTTGGCTGTGGTTCCGGCATTCTGGCGTTCGCGGCGCTGAAACTGGGTGCAGCAAAAGCCa                                                                                                     <  2:264221/140‑1 (MQ=255)                                aCGGGTACCCATCCAACCACCTCTCTGTGCCTGCAATGGCTCGACAGCCTCGATTTAACCGGTAAAACAGTCATCGACTTTGGCTGTGGTTCCGGCATTCTGGCGTTCGCGGCGCTGAAACTGGGTGCAGCAAAAGCCAt                                                                                                    <  2:786830/140‑1 (MQ=255)                                 cGGGTACCCATCCAACCACCTCTCTGTGCCTGCAATGGCTCGACAGCCTCGATTTAACCGGTAAAACAGTCATCGACTTTGGCTGTGGTTCCGGCATTCTGGCGTTCGCGGCGCTGAAACTGGGTGCAGCAAAAGCCAtt                                                                                                   <  2:274114/140‑1 (MQ=255)                                 cGGGTACCCATCCAACCACCTCTCTGTGCCTGCAATGGCTCGACAGCCTCGATTTAACCGGTAAAACAGTCATCGACTTTGGCTGTGGTTCCGGCATTCTGGCGTTCGCGGCGCTGAAACTGGGTGCAGCAAAAGCCAtt                                                                                                   <  1:67138/140‑1 (MQ=255)                                       cccATCCAACCACCTCTCTGTGCCTGCAATGGCTCGACAGCCTCGATTTAACCGGTAAAACAGTCATCGACTTTGGCTGTGGTTCCGGCATTCTGGCGTTCGCGGCGCTGAAACTGGGTGCAGCAAAAGCCATTGGTAtt                                                                                             >  1:321788/1‑140 (MQ=255)                                            ccAACCACCTCTCTGTGCCTGCAATGGCTCGACAGCCTCGATTTAACCGGTAAAACAGTCATCGACTTTGGCTGTGGTTCCGTCATTCTGGCGTTCGCGGCGCTGAAACTGGGTGCAGCAAAAGCCATTGGTATTGatat                                                                                        <  2:70432/140‑1 (MQ=255)                                            ccAACCACCTCTCTGTGCCTGCAATGGCTCGACAGCCTCGATTTAACCGGTAAAACAGTCATCGACTTTGGCTGTGGTTCCGGCATTCTGGCGTTCGCGGCGCTGAAACTGGGTGCAGCAAAAGCCATTGGTATTGatat                                                                                        <  2:61198/140‑1 (MQ=255)                                             cAACCACCTCTCTGTGCCTGCAATGGCTCGACAGCCTCGATTTAACCGGTAAAACAGTCATCGACTTTGGCTGTGGTTCCGGCATTCTGGCGTTCGCGGCGCTGAAACTGGGTGCAGCAAAAGCCATTGGTATTGATATc                                                                                       >  2:680677/1‑140 (MQ=255)                                              aaCCACCTCTCTGTGCCTGCAATGGCTCGACAGCCTCGATTTAACCGGTAAAACAGTCATCGACTTTGGCTGTGGTTCCGGCATTCTGGCGTTCGCGGCGCTGAAACTGGGTGCAGCAAAAGc                                                                                                       >  1:589520/1‑123 (MQ=255)                                              aaCCACCTCTCTGTGCCTGCAATGGCTCGACAGCCTCGATTTAACCGGTAAAACAGTCATCGACTTTGGCTGTGGTTCCGGCATTCTGGCGTTCGCGGCGCTGAAACTGGGTGCAGCAAAAGc                                                                                                       <  2:589520/123‑1 (MQ=255)                                                  accTCTCTGTGCCTGCAATGGCTCGACAGCCTCGATTTAACCGGTAAAACAGTCATCGACTTTGGCTGTGGTTCCGGCATTCTGGCGTTCGCGGCGCTGAAACTGGGTGCAGCAAAAGCCATTGGTATTGATATCGATcc                                                                                  >  1:269691/1‑140 (MQ=255)                                                             ccTGCAATGGCTCAACAGCCTCGATTTAACCGGTAAAACAGTCATCGACTTTGGCTGTGGTTCCGGCATTCTGGCGTTCGCGGCGCTGAAACTGGGTGCAGCAAAAGCCATTGGTATTGATATCGATCCGCAGGCGATTc                                                                       >  2:862611/1‑140 (MQ=255)                                                               tGCAATGGCTCGACAGCCTCGATTTAACCGGTAAAACAGTCATCGACTTTGGCTGTGGTTCCGGCATTCTGGCGTTCGCGGCGCTGAAACTGGGTGCAGCAAAAGCCATTGGTATTGATATCGATCCGCAGGCGATTCAg                                                                     >  1:217735/1‑140 (MQ=255)                                                                gCAATGGCTCGACAGCCTCGATTTAACCGGTAAAACAGTCATCGACTTTGGCTGTGGTTCCGGCATTCTGGCGTTCGCGGCGCTGAAACTGGGTGCAGCAAAAGCCATTGGTATTGATATCGATCCGCAGGCGATTCAgg                                                                    <  2:217735/140‑1 (MQ=255)                                                                 cAATGGCTCGACAGCCTCGATTTAACCGGTAAAACAGTCATCGACTTTGGCTGTGGTTCCGGCATTCTGGCGTTCGCGGCGCTGAAACTTGGTGCAGCAAAAGCCATTGGTATTGATATCGATCCGCAgg                                                                             <  1:674832/130‑1 (MQ=255)                                                                 cAATGGCTCGACAGCCTCGATTTAACCGGTAAAACAGTCATCGACTTTGGCTGTGGTTCCGGCATTCTGGCGTTCGCGGCGCTGAAACTTGGTGCAGCAAAAGCCATTGGTATTGATATCGATCCGCAgg                                                                             >  2:674832/1‑130 (MQ=255)                                                                 cAATGGCTCGACAGCCTCGATTTAACCGGTAAAACAGTCATCGACTTTGGCTGTGGTTCCGGCATTCTGGCGTTCGCGGCGCTGAAACTGGGTGCAGCAAAAGCCATTGGTATTGATATCGATCCGCAGGCGATTCAGGc                                                                   >  2:542063/1‑140 (MQ=255)                                                                 cAATGGCTCGACAGCCTCGATTTAACCGGTAAAACAGTCATCGACTTTGGCTGTGGTTCCGGCATTCTGGCGTTCGCGGCGCTGAAACTGGGTGCAGCAAAAGCCATTGGTATTGATATCGATCCGCAGGCGATTCAGGc                                                                   >  1:101596/1‑140 (MQ=255)                                                                 cAATGGCTCGACAGCCTCGATTTAACCGGTAAAACAGTCATCGACTTTGGCTGTGGTTCCGGCATTCTGGCGTTCGCGGCGCTGAAACTGGGTGCAGCAAAAGCCATTGGTATTGATATCGATCCGCAGGCGATTCAGGc                                                                   >  1:451011/1‑140 (MQ=255)                                                                   aTGGCTCGACAGCCTCGATTTAACCGGTAAAACAGTCATCGACTTTGGCTGTGGTTCCGGCATTCTGGCGTTCGCGGCGCTGAAACTGGGTGCAGCAAAAGCCATTGGTATTGATATCGATCCGCAGGCGATTCAGGCCa                                                                 >  2:125344/1‑140 (MQ=255)                                                                     ggCTCGACAGCCTCGATTTAACCGGTAAAACAGTCATCGACTTTGGCTGTGGTTCCGGCATTCTGGCGTTCGCGGCGCTGAAACTGGGTGCAGCAAAAGCCATTGGTATTGATATCGATCCGCAGGCGATTCAGGCCAGc                                                               >  2:567059/1‑140 (MQ=255)                                                                       cTCGACAGCCTCGATTTAACCGGTAAAACAGTCATCGACTTTGGCTGTGGTTCCGGCATTCTGGCGTTCGCGGCGCTGAAACTGGGTGCAGCAAAAGCCATTGGTATTGATATCGATCCGCAGGCGATTCAGGCCAGCcg                                                             <  1:117171/140‑1 (MQ=255)                                                                           aCAGCCTCGATTTAACCGGTAAAACAGTCATCGACTTTGGCTGTGGTTCCGGCATTCTGGCGTTCGCGGCGCTGAAACTGGGTGCAGCAAAAGCCATTGGTATTGATATCGATCCGCAGGCGATTCAGGCCAGCCGCGAt                                                         <  2:101596/140‑1 (MQ=255)                                                                            cAGCCTCGATTTAACCGGTAAAACAGTCATCGACTTTGGCTGTGGTTCCGGCATTCTGGCGTTCGCGGCGCTGAAACTGGGTGCAGCAAAAGCCATTGGTATTGATATCGATCCGCAGGCGATTCAGGCCAGCCGCGATa                                                        <  2:574653/140‑1 (MQ=255)                                                                                cTCGATTTAACCGGTAAAACAGTCATCGACTTTGGCTGTGGTTCCGGCATTCTGGCGTTCGCGGCGCTGAAACTGGGTGCAGCAAAAGCCATTGGTATTGATATCGATCCGCAGGCGATTCAGGCCAGCCGCGATAACGc                                                    >  1:39152/1‑140 (MQ=255)                                                                                cTCGATTTAACCGGTAAAACAGTCATCGACTTTGGCTGTGGTTCCGGCATTCTGGCGTTCGCGGCGCTGAAACTGGGTGCAGCAAAAGCCATTGGTATTGATATCGATCCGCAGGCGATTCAGGCCAGCCGCGATAACGc                                                    >  1:602876/1‑140 (MQ=255)                                                                                   gATTTAACCGGTAAAACAGTCATCGACTTTGGCTGTGGTTCCGGCATTCTGGCGTTCGCGGCGCTGAAACTGGGTGCAGCAAAAGCCATTGGTATTGATATCGATCCGCAGGCGATTCAGGCCAGCCGCGATAACGCCGa                                                 <  2:833917/140‑1 (MQ=255)                                                                                    aTTTAACCGGTAAAACAGTCATCGACTTTGGCTGTGGTTCCGGCATTCTGGCGTTCGCGGCGCTGAAACTGGGTGCAGCAAAAGCCATTGGTATTGATATCGATCCGCAGGCGATTCAGGCCAGCCGCGATAACGCCGaa                                                <  2:602876/140‑1 (MQ=255)                                                                                       tAACCGGTAAAACAGTCATCGACTTTGGCTGTGGTTCCGGCATTCTGGCGTTCGCGGCGCTGAAACTGGGTGCAGCAAAAGCCATTGGTATTGATATCGATCCGCAGGCGATTCAGGCCAGCCGCGATAACGCCGAACGt                                             <  2:321788/140‑1 (MQ=255)                                                                                         aCCGGTAAAACAGTCATCGACTTTGGCTGTGGTTCCGGCATTCTGGCGTTCGCGGCGCTGAAACTGGGTGCAGCAAAAGCCATTGGTATTGATATCGATCCGCAGGCGATTCAGGCCAGCCGCGATAACGCCGAACGTaa                                           >  2:922450/1‑140 (MQ=255)                                                                                           cGGTAAAACAGTCATCGACTTTGGCTGTGGTTCCGGCATTCTGGCGTTCGCGGCGCTGAAACTGGGTGCAGCAAAAGCCATTGGTATTGATATCGATCCGCAGGCGATTCAGGCCAGCCGCGATAACGCCGAACGTAATg                                         <  1:125344/140‑1 (MQ=255)                                                                                            ggTAAAACAGTCATCGACTTTGGCTGTGGTTCCGGCATTCTGGCGTTCGCGGCGCTGAAACTGGGTGCAGCAAAAGCCATTGGTATTGATATCGATCCGCAGGCGATTCAGGCCAGCCGCGATAACGCCGAACGTAATgg                                        <  2:647119/140‑1 (MQ=255)                                                                                                     gTCATCGACTTTGGCTGTGGTTCCGGCATTCTGGCGTTCGCGGCGCTGAAACTGGGTGCAGCAAAAGCCATTGGTATTGATATCGATCCGCAGGCGATTCAGGCCAGCCGCGATAACGCCGAACGTAATGGCGTTTCTGa                               <  2:306382/140‑1 (MQ=255)                                                                                                       cATCGACTTTGGCTGTGGTTCCGGCATTCTGGCGTTCGCGGCGCTGAAACTGGGTGCAGCAAAAGCCATTGGTATTGATATCGATCCGCAGGCGATTCAGGCCAGCCGCGATAACGCCGAACGTAATGGCGTTTCTGAcc                             >  1:565966/1‑140 (MQ=255)                                                                                                        aTCGACTTTGGCTGTGGTTCCGGCATTCTGGCGTTCGCGGCGCTGAAACTGGGTGCAGCAAAAGCCATTGGTATTGATATCGATCCGCAGGCGATTCAGGCCAGCCGCGATAACGCCGAACGTAATGGCGTTTCTGAcc                             <  2:21070/139‑1 (MQ=255)                                                                                                        aTCGACTTTGGCTGTGGTTCCGGCATTCTGGCGTTCGCGGCGCTGAAACTGGGTGCAGCAAAAGCCATTGGTATTGATATCGATCCGCAGGCGATTCAGGCCAGCCGCGATAACGCCGAACGTAATGGCGTTTCTGAcc                             >  1:21070/1‑139 (MQ=255)                                                                                                        aTCGACTTTGGCTGTGGTTCCGGCATTCTGGCGTTCGCGGCGCTGAAACTGGGTGCAGCAAAAGCCATTGGTATTGATATCGATCCGCAGGCGATTCAGGCCAGCCGCGATAACGCCGAACGTAATGGCGTTTCTGACCg                            >  1:643040/1‑140 (MQ=255)                                                                                                        aTCGACTTTGGCTGTGGTTCCGACATTCTGGCGTTCGCGGCGCTGAAACTGGGTGCAGCAAAAGCCATTGGTATTGATATCGATCCGCAGGCGATTCAGGCCAGCCGCGATAACGCCGAACGTAATGGCGTTTCTGACCg                            >  2:59820/1‑140 (MQ=255)                                                                                                          cGACTTTGGCTGTGGTTCCGGCATTCTGGCGTTCGCGGCGCTGAAACTGGGTGCAGCAAAAGCCATTGGTATTGATATCGATCCGCAGGCGATTCAGGCCAGCCGCGATAACGCCGAACGTAATGGCGTTTCTGACCGTc                          <  2:39152/140‑1 (MQ=255)                                                                                                            aCTTTGGCTGTGGTTCCGGCATTCTGGCGTTCGCGGCGCTGAAACTGGGTGCAGCAAAAGCCATTGGTATTGATATCGATCCGCAGGCGATTCAGGCCAGCCGCGATAACGCCGAACGTAATGGCGTTTCTGACCGTCTg                        <  1:567059/140‑1 (MQ=255)                                                                                                               ttGGCTGTGGTTCCGGCATTCTGGCGTTCGCGGCGCTGAAACTGGGTGCAGCAAAAGCCATTGGTATTGATATCGATCCGCAGGCGATTCAGGCCAGCCGCGATAACGCCGAACGTAATGGCGTTTCTGACCGTCTGGaa                     <  1:862611/140‑1 (MQ=255)                                                                                                                tGGCTGTGGTTCCGGCATTCTGGCGTTCGCGGCGCTGAAACTGGGTGCAGCAAAAGCCATTGGTATTGATATCGATCCGCAGGCGATTCAGGCCAGCCGCGATAACGCCGAACGTAATGGCGTTTCTGACCGTc                          <  2:708293/134‑1 (MQ=255)                                                                                                                tGGCTGTGGTTCCGGCATTCTGGCGTTCGCGGCGCTGAAACTGGGTGCAGCAAAAGCCATTGGTATTGATATCGATCCGCAGGCGATTCAGGCCAGCCGCGATAACGCCGAACGTAATGGCGTTTCTGACCGTc                          >  1:708293/1‑134 (MQ=255)                                                                                                                      tgGTTCCGGCATTCTGGCGTTCGCGGCGCTGAAACTGGGTGCAGCAAAAGCCATTGGTATTGATATCGATCCGCAGGCGATTCAGGCCAGCCGCGATAACGCCGAACGTAATGGCGTTTCTGACCGTCTGGAACTCTACt              <  1:407791/140‑1 (MQ=255)                                                                                                                       ggTTCCGGCATTCTGGCGTTCGCGGCGCTGAAACTGGGTGCAGCAAAAGCCATTGGTATTGATATCGATCCGCAGGCGATTCAGGCCAGCCGCGATAACGCCGAACGTAATGGCGTTTCTGACCGTCTGGAACTCTACtt             >  2:944735/1‑140 (MQ=255)                                                                                                                           ccGGCATTCTGGCGTTCGCGGCGCTGAAACTGGGTGCAGCAAAAGCCATTGGTATTGATATCGATCCGCAGGCGATTCAGGCCAGCCGCGATAACGCCGAACGTAATGGCGTTTCTGACCGTCTGGAACTCTACTTACCg         <  1:542063/140‑1 (MQ=255)                                                                                                                           ccGACATTCTGGCGTTCGCGGCGCTGAAACTGGGTGCAGCAAAAGCCATTGGTATTGATATCGATCCGCAGGCGATTCAGGCCAGCCGCGATAACGCCGAACGTAATGGCGTTTCTGACCGTCTGGAACTCTACTTACCg         <  1:59820/140‑1 (MQ=255)                                                                                                                            cGGCATTCTGGCGTTCGCGGCGCTGAAACTGGGTGCAGCAAAAGCCATTGGTATTGATATCGATCCGCAGGCGATTCAGGCCAGCCGCGATAACGCCGAACGTAATGGCGTTTCTGACCGTCTGGAACTCTACTTACCGa        <  2:633477/140‑1 (MQ=255)                                                                                                                                aTTCTGGCGTTCGCGGCGCTGAAACTGGGTGCAGCAAAAGCCATTGGTATTGATATCGATCCGCAGGCGATTCAGGCCAGCCGCGATAACGCCGAACGTAATGGCGTTTCTGACCGTCTGGAACTCTACTTACCGAAAGa    <  2:565966/140‑1 (MQ=255)                                                                                                                                 ttCTGGCGTTCGCGGCGCTGAAACTGGGTGCAGCAAAAGCCATTGGTATTGATATCGATCCGCAGGCGATTCAGGCCAGCCGCGATAACGCCGAACGTAATGGCGTTTCTGACCGTCTGGAACTCTACTTACCGAAAGAt   <  1:334902/140‑1 (MQ=255)                                                                                                                                 ttCTGGCGTTCGCGGCGCTGAAACTGGGTGCAGCAAAAGCCATTGGTATTGATATCGATCCGCAGGCGATTCAGGCCAGCCGCGATAACGCCGAACGTAATGGCGTTTCTGACCGTCTGGAACTCTACTTACCGAAAGAt   <  1:213532/140‑1 (MQ=255)                                                                                                                                  tCTGGCGTTCGCGGCGCTGAAACTGGGTGCAGCAAAAGCCATTGGTATTGATATCGATCCGCAGGCGATTCAGGCCAGCCGCGATAACGCCGAACGTAATGGCGTTTCTGACCGTCTGGAACTCTACTTACCGAAAGATc  >  1:777502/1‑140 (MQ=255)                                                                                                                                  tCTGGCGTTCGCGGCGCTGAAACTGGGTGCAGCAAAAGCCATTGGTATTGATATCGATCCGCAGGCGATTCAGGCCAGCCGCGATAACGCCGAACGTAATGGCGTTTCTGACCGTCTGGAACTCTACTTACCGAAAGATc  >  2:602609/1‑140 (MQ=255)                                                                                                                                         |                                                                                                                                     CGTGATGTTAGATCCAGGGCTGGCGTTTGGTACGGGTACCCATCCAACCACCTCTCTGTGCCTGCAATGGCTCGACAGCCTCGATTTAACCGGTAAAACAGTCATCGACTTTGGCTGTGGTTCCGGCATTCTGGCGATCGCGGCGCTGAAACTGGGTGCAGCAAAAGCCATTGGTATTGATATCGATCCGCAGGCGATTCAGGCCAGCCGCGATAACGCCGAACGTAATGGCGTTTCTGACCGTCTGGAACTCTACTTACCGAAAGATC  >  NC_000913/3409453‑3409721

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑