Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,541,606	T→C	G400G (GGT→GGC)	feoB →	Fe(2(+)) transporter FeoB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,541,606	0	T	C	100.0%	279.0 / NA	77	G400G (GGT→GGC)	feoB	Fe(2(+)) transporter FeoB
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (42/35);  total (42/35)

CCGCAGATTGGCATGATGTACCTGTTCCTCTCCTTCCTTGAGGACTCCGGGTATATGGCGCGTGCGGCGTTTGTGATGGACCGTCTGATGCAGGCGCTGGGCTTGCCGGGGAAATCCTTTGTGCCGCTGATCGTCGGTTTCGGTTGTAACGTACCGTCGGTAATGGGTGCACGTACGCTTGATGCACCGCGTGAACGTCTGATGACCATCATGATGGCACCGTTTATGTCCTGCGGCGCGCGTCTGGCTATCTTCGCAGTATTCGCGGCTG  >  NC_000913/3541469‑3541739                                                                                                                                          |                                                                                                                                      ccGCAGATTGGCATGATGTACCTGTTCCTCTCCTTCCTTGAGGACTCCGGGTATATGGCGCGTGCGGCGTTTGTGATGGACCGTCTGATGCAGGCGCTGGGCTTGCCGGGGAAATCCTTTGTGCCGCTGATCGtcggctt                                                                                                                                     <  2:913251/140‑1 (MQ=255)  cGCAGATTGGCATGATGTACCTGTTCCTCTCCTTCCTTGAGGACTCCGGGTATATGGCGCGTGCGGCGTTTGTGATGGACCGTCTGATGCAGGCGCTGGGCTTGCCGGGGAAATCCTTTGTGCCGCTGATCGtcggcttc                                                                                                                                    >  1:948370/1‑140 (MQ=255)    cAGATTGGCATGATGTACCTGTTCCTCTCCTTCCTTGAGGACTCCGGGTATATGGCGCGTGCGGCGTTTGTGATGGACCGTCTGATGCAGGCGCTGGGCTTGCCGGGGAAATCCTTTGTGCCGCTGATCGtcggcttcgg                                                                                                                                  <  2:131792/140‑1 (MQ=255)       aTTGGCATGATGTACCTGTTCCTCTCCTTCCTTGAGGACTCCGGGTATATGGCGCGTGCGGCGTTTGTGATGGACCGTCTGATGCAGGCGCTGGGCTTGCCGGGGAAATCCTTTGTGCCGCTGATCGTCGGCTTCGGTTg                                                                                                                               <  1:220568/140‑1 (MQ=255)            cATGATGTACCTGTTCCTCTCCTTCCTTGAGGACTCCGGGTATATGGCGCGTGCGGCGTTTGTGATGGACCGTCTGATGCAGGCGCTGGGCTTGCCGGGGAAATCCTTTGTGCCGCTGATCGtcggcttcgg                                                                                                                                  >  1:15370/1‑132 (MQ=255)            cATGATGTACCTGTTCCTCTCCTTCCTTGAGGACTCCGGGTATATGGCGCGTGCGGCGTTTGTGATGGACCGTCTGATGCAGGCGCTGGGCTTGCCGGGGAAATCCTTTGTGCCGCTGATCGtcggcttcgg                                                                                                                                  <  2:15370/132‑1 (MQ=255)            cATGATGTACCTGTTCCTCTCCTTCCTTGAGGACTCCGGGTATATGGCGCGTGCGGCGTTTGTGATGGACCGTCTGATGCAGGCGCTGGGCTTGCCGGGGAAATCCTTTGTGCCGCTGATCGTCGGCTTCGGTTGTAACg                                                                                                                          >  1:914512/1‑140 (MQ=255)              tgatgTACCTGTTCCTCTCCTTCCTTGAGGACTCCGGGTATATGGCGCGTGCGGCGTTTGTGATGGACCGTCTGATGCAGGCGCTGGGCTTGCCGGGGAAATCCTTTGTGCCGCTGATCGTCGGCTTCGGTTGTAACGTa                                                                                                                        <  2:37194/140‑1 (MQ=255)                 tgTACCTGTTCCTCTCCTTCCTTGAGGACTCCGGGTATATGGCGCGTGCGGCGTTTGTGATGGACCGTCTGATGCAGGCGCTGGGCTTGCCGGGGAAATCCTTTGTGCCGCTGATCGTCGGCTTCGGTTGTAACGTACCg                                                                                                                     <  2:357342/140‑1 (MQ=255)                 tgTACCTGTTCCTCTCCTTCCTTGAGGACTCCGGGTATATGGCGCGTGCGGCGTTTGTGATGGACCGTCTGATGCAGGCGCTGGGCTTGCCGGGGAAATCCTTTGTGCCGCTGATCGTCGGCTTCGGTTGTAACGTACCg                                                                                                                     <  2:182634/140‑1 (MQ=255)                     ccTGTTCCTCTCCTTCCTTGAGGACTCCGGGTATATGGCGCGTGCGGCGTTTGTGATGGACCGTCTGATGCAGGCGCTGGGCTTGCCGGGGAAATCCTTTGTGCCGCTGATCGTCGGCTTCGGTTGTAACGTACCGTCgg                                                                                                                 >  1:127085/1‑140 (MQ=255)                     ccTGTTCCTCTCCTTCCTTGAGGACTCCGGGTATATGGCGCGTGCGGCGTTTGTGATGGACCGTCTGATGCAGGCGCTGGGCTTGCCGGGGAAATCCTTTGTGCCGCTGATCGTCGGCTTCGGTTGTAACGTACCGTCgg                                                                                                                 >  1:140968/1‑140 (MQ=255)                      cTGTTCCTCTCCTTCCTTGAGGACTCCGGGTATATGGCGCGTGCGGCGTTTGTGATGGACCGTCTGATGCAGGCGCTGGGCTTGCCGGGGAAATCCTTTGTGCCGCTGATCGTCGGCTTCGGTTGTAACGTACCGTCGGt                                                                                                                <  2:140968/140‑1 (MQ=255)                       tGTTCCTCTCCTTCCTTGAGGACTCCGGGTATATGGCGCGTGCGGCGTTTGTGATGGACCGTCTGATGCAGGCGCTGGGCTTGCCGGGGAAATCCTTTGTGCCGCTGATCGTCGGCTTCGGTTGTAACGTACCGTCGGTa                                                                                                               >  1:819005/1‑140 (MQ=255)                         ttCCTCTCCTTCCTTGAGGACTCCGGGTATATGGCGCGTGCGGCGTTTGTGATGGACCGTCTGATGCAGGCGCTGGGCTTGCCGGGGAAATCCTTTGTGCCGCTGATCGTCGGCTTCGGTTGTAACGTACCGTCGGTAAt                                                                                                             <  2:432417/140‑1 (MQ=255)                         ttCCTCTCCTTCCTTGAGGACTCCGGGTATATGGCGCGTGCGGCGTTTGTGATGGACCGTCTGATGCAGGCGCTGGGCTTGCCGGGGAAATCCTTTGTGCCGCTGATCGTCGGCTTCGGTTGTAACGTACCGTCGGTAAt                                                                                                             <  2:480181/140‑1 (MQ=255)                          tCCTCTCCTTCCTTGAGGACTCCGGGTATATGGCGCGTGCGGCGTTTGTGATGGACCGTCTGATGCAGGCGCTGGGCTTGCCGGGGAAATCCTTTGTGCCGCTGATCGTCGGCTTCGGTTGTAACGTACCGTCGGTAATg                                                                                                            >  1:244988/1‑140 (MQ=255)                           ccTCTCCTTCCTTGAGGACTCCGGGTATATGGCGCGTGCGGCGTTTGTGATGGACCGTCTGATGCAGGCGCTGGGCTTGCCGGGGAAATCCTTTGTGCCGCTGATCGTCGGCTTCGGTTGTAACGTACCGTCGGTAATgg                                                                                                           >  1:161699/1‑140 (MQ=255)                           ccTCTCCTTCCTTGAGGACTCCGGGTATATGGCGCGTGCGGCGTTTGTGATGGACCGTCTGATGCAGGCGCTGGGCTTGCCGGGGAAATCCTTTGTGCCGCTGATCGTCGGCTTCGGTTGTAACGTACCGTCGGTAATgg                                                                                                           >  1:73425/1‑140 (MQ=255)                           ccTCTCCTTCCTTGAGGACTCCGGGTATATGGCGCGTGCGGCGTTTGTGATGGACCGTCTGATGCAGGCGCTGGGCTTGCCGGGGAAATCCTTTGTGCCGCTGATCGTCGGCTTCGGTTGTAACGTACCGTCGGTAATgg                                                                                                           >  1:517646/1‑140 (MQ=255)                            ctctCCTTCCTTGAGGACTCCGGGTATATGGCGCGTGCGGCGTTTGTGATGGACCGTCTGATGCAGGCGCTGGGCTTGCCGGGGAAATCCTTTGTGCCGCTGATCGTCGGCTTCGGTTGTAACGTACCGTCGGTAATggg                                                                                                          <  2:490768/140‑1 (MQ=255)                             tctcCTTCCTTGAGGACTCCGGGTATATGGCGCGTGCGGCGTTTGTGATGGACCGTCTGATGCAGGCGCTGGGTTTGCCGGGGAAATCCTTTGTGCCGCTGATCGTCGGCTTCGGTTGTAACGTACCGTCGGTAATgggg                                                                                                         >  2:247234/1‑139 (MQ=255)                             tctcCTTCCTTGAGGACTCCGGGTATATGGCGCGTGCGGCGTTTGTGATGGACCGTCTGATGCAGGCGCTGGGCTTGCCGGGGAAATCCTTTGTGCCGCTGATCGTCGGCTTCGGTTGTAACGTACCGTCGGTAATGGGt                                                                                                         <  2:517646/140‑1 (MQ=255)                              ctcCTTCCTTGAGGACTCCGGGTATATGGCGCGTGCGGCGTTTGTGATGGACCGTCTGATGCAGGCGCTGGGCTTGCCGGGGAAATCCTTTGTGCCGCTGATCGTCGGCTTCGGTTGTAACGTACCGTCGGTAATGGGTg                                                                                                        >  2:735471/1‑140 (MQ=255)                               tccttcctTGAGGACTCCGGGTATATGGCGCGTGCGGCGTTTGTGATGGACCGTCTGATGCAGGCGCTGGGCTTGCCGGGGAAATCCTTTGTGCCGCTGATCGTCGGCTTCGGTTGTAACGTACCGTCGGTAATGGGTGc                                                                                                       >  2:235627/1‑140 (MQ=255)                                     cttGAGGACTCCGGGTATATGGCGCGTGCGGCGTTTGTGATGGACCGTCTGATGCAGGCGCTGGGCTTGCCGGGGAAATCCTTTGTGCCGCTGATCGTCGGCTTCGGTTGTAACGTACCGTCGGTAATGGGTGCACGTAc                                                                                                 <  2:897254/140‑1 (MQ=255)                                         aGGACTCCGGGTATATGGCGCGTGCGGCGTTTGTGATGGACCGTCTGATGCAGGCGCTGGGCTTGCCGGGGAAATCCTTTGTGCCGCTGATCGTCGGCTTCGGTTGTAACGTACCGTCGGTAATGGGTGCACGTACGCtt                                                                                             >  2:157335/1‑140 (MQ=255)                                           gACTCCGGGTATATGGCGCGTGCGGCGTTTGTGATGGACCGTCTGATGCAGGCGCTGGGCTTGCCGGGGAAATCCTTTGTGCCGCTGACCGTCGGCTTCGGTTGTAACGTACCGTCGGTAATGGGTGCACGTACGCTTGa                                                                                           >  1:5028/1‑140 (MQ=255)                                             cTCCGGGTATATGGCGCGTGCGGCGTTTGTGATGGACCGTCTGATGCAGGCGCTGGGCTTGCCGGGGAAATCCTTTGTGCCGCTGATCGTCGGCTTCGGTTGTAACGTACCGTCGGTAATGGGTGCACGTACGCTTGATg                                                                                         <  2:244988/140‑1 (MQ=255)                                             cTCCGGGTATATGGCGCGTGCGGCGTTTGTGATGGACCGTCTGATGCAGGCGCTGGGCTTGCCGGGGAAATCCTTTGTGCCGCTGATCGTCGGCTTCGGTTGTAACGTACCGTCGGTAATGGGTGCACGTACGCTTGATg                                                                                         >  1:183656/1‑140 (MQ=255)                                              tCCGGGTATATGGCGCGTGCGGCGTTTGTGATGGACCGTCTGATGCAGGCGCTGGGCTTGCCGGGGAAATCCTTTGTGCCGCTGATCGTCGGCTTCGGTTGTAACGTACCGTCGGTAATGGGTGCACGTACGCTTGATGc                                                                                        >  2:426441/1‑140 (MQ=255)                                              tCCGGGTATATGGCGCGTGCGGCGTTTGTGATGGACCGTCTGATGCAGGCGCTGGGCTTGCCGGGGAAATCCTTTGTGCCGCTGATCGTCGGCTTCGGTTGTAACGTACCGTCGGTAATGGGTGCACGTACGCTTGATGc                                                                                        >  1:844545/1‑140 (MQ=255)                                              tCCGGGTATATGGCGCGTGCGGCGTTTGTGATGGACCGTCTGATGCAGGCGCTGGGCTTGCCGGGGAAATCCTTTGTGCCGCTGATCGTCGGCTTCGGTTGTAACGTACCGTCGGTAATGGGTACACGTACGCTTGATGc                                                                                        >  1:347894/1‑140 (MQ=255)                                                cGGGTATATGGCGCGTGCGGCGTTTGTGATGGACCGTCTGATGCAGGCGCTGGGCTTGCCGGGGAAATCCTTTGTGCCGCTGATCGTCGGCTTCGGTTGTAACGTACCGTCGGTAATGGGTGCACGTACGCTTGATGCAc                                                                                      >  1:776348/1‑140 (MQ=255)                                                    tataTGGCGCGTGCGGCGTTTGTGATGGACCGTCTGATGCAGGCGCTGGGCTTGCCGGGGAAATCCTTTGTGCCGCTGATCGTCGGCTTCGGTTGTAACGTACCGTCGGTAATGGGTGCACGTACGCTTGATGCACcgcg                                                                                  <  2:543837/140‑1 (MQ=255)                                                    tataTGGCGCGTGCGGCGTTTGTGATGGACCGTCTGATGCAGGCGCTGGGCTTGCCGGGGAAATCCTTTGTGCCGCTGACCGTCGGCTTCGGTTGTAACGTACCGTCGGTAATGGGTGCACGTACGCTTGATGCACcgcg                                                                                  <  2:5028/140‑1 (MQ=255)                                                        tGGCGCGTGCGGCGTTTGTGATGGACCGTCTGATGCAGGCGCTGGGCTTGCCGGGGAAATCCTTTGTGCCGCTGATCGTCGGCTTCGGTTGTAACGTACCGTCGGTAATGGGTGCACGTACGCTTGATGCACCGCGTGaa                                                                              <  1:470667/140‑1 (MQ=255)                                                        tGGCGCGTGCGGCGTTTGTGATGGACCGTCTGATGCAGGCGCTGGGCTTGCCGGGGAAATCCTTTGTGCCGCTGATCGTCGGCTTCGGTTGTAACGTACCGTCGGTAATGGGTGCACGTACGCTTGATGCACCGCGTGaa                                                                              >  1:189050/1‑140 (MQ=255)                                                        tGGCGCGTGCGGCGTTTGTGATGGACCGTCTGATGCAGGCGCTGGGCTTGCCGGGGAAATCCTTTGTGCCGCTGATCGTCGGCTTCGGTTGTAACGTACCGTCGGTAATGGGTGCACGTACGCTTGATGCACCGCGTGaa                                                                              <  2:695173/140‑1 (MQ=255)                                                        tGGCGCGTGCGGCGTTTGTGATGGACCGTCTGATGCAGGCGCTGGGCTTGCCGGGGAAATCCTTTGTGCCGCTGATCGTCGGCTTCGGTTGTAACGTACCGTCGGTAATGGGTGCACGTACGCTTGATGCACCGCGTGaa                                                                              >  2:912485/1‑140 (MQ=255)                                                         ggCGCGTGCGGCGTTTGTGATGGACCGTCTGATGCAGGCGCTGGGCTTGCCGGGGAAATCCTTTGTGCCGCTGATCGTCGGCTTCGGTTGTAACGTACCGTCGGTAATGGGTGCACGTACGCTTGATGCACCGCGTGAAc                                                                             <  1:912485/140‑1 (MQ=255)                                                            gcgTGCGGCGTTTGTGATGGACCGTCTGATGCAGGCGCTGGGCTTGCCGGGGAAATCCTTTGTGCCGCTGATCGTCGGCTTCGGTTGTAACGTACCGTCGGTAATGGGTGCACGTACGCTTGATGCACCGCGTGaa                                                                              >  1:947024/1‑136 (MQ=255)                                                            gcgTGCGGCGTTTGTGATGGACCGTCTGATGCAGGCGCTGGGCTTGCCGGGGAAATCCTTTGTGCCGCTGATCGTCGGCTTCGGTTGTAACGTACCGTCGGTAATGGGTGCACGTACGCTTGATGCACCGCGTGaa                                                                              <  2:947024/136‑1 (MQ=255)                                                            gcgTGCGGCGTTTGTGATGGACCGTCTGATGCAGGCGCTGGGCTTGCCGGGGAAATCCTTTGTGCCGCTGATCGTCGGCTTCGGTTGTAACGTACCGTCGGTAATGGGTGCACGTACGCTTGATGCACCGCGTGAACGTc                                                                          >  1:705579/1‑140 (MQ=255)                                                             cgTGCGGCGTTTGTGATGGACCGTCTGATGCAGGCGCTGGGCTTGCCGGGGAAATCCTTTGTGCCGCTGATCGTCGGCTTCGGTTGTAACGTACCGTCGGTAATGGGTGCACGTACGCTTGATGCACCGCGTGAACGTCt                                                                         >  1:72741/1‑140 (MQ=255)                                                             cgTGCGGCGTTTGTGATGGACCGTCTGATGCAGGCGCTGGGCTTGCCGGGGAAATCCTTTGTGCCGCTGATCGTCGGCTTCGGTTGTAACGTACCGTCGGTAATGGGTGCACGTACGCTTGATGCACCGCGTGAACGTCt                                                                         >  1:238941/1‑140 (MQ=255)                                                             cgTGCGGCGTTTGTGATGGACCGTCTGATGCAGGCGCTGGGCTTGCCGGGGAAATCCTTTGTGCCGCTGATCGTCGGCTTCGGTTGTAACGTACCGTCGGTAATGGGTGCACGTACGCTTGATGCACCGCGTGAACGTCt                                                                         >  1:908785/1‑140 (MQ=255)                                                              gTGCGGCGTTTGTGATGGACCGTCTGATGCAGGCGCTGGGCTTGCCGGGGAAATCCTTTGTGCCGCTGATCGTCGGCTTCGGTTGTAACGTACCGTCGGTAATGGGTGCACGTACGCTTGATGCACCGCGTGaa                                                                              <  1:627759/134‑1 (MQ=255)                                                              gTGCGGCGTTTGTGATGGACCGTCTGATGCAGGCGCTGGGCTTGCCGGGGAAATCCTTTGTGCCGCTGATCGTCGGCTTCGGTTGTAACGTACCGTCGGTAATGGGTGCACGTACGCTTGATGCACCGCGTGaa                                                                              >  2:627759/1‑134 (MQ=255)                                                                gcggcgTTTGTGATGGACCGTCTGATGCAGGCGCTGGGCTTGCCGGGGAAATCCTTTGTGCCGCTGATCGTCGGCTTCGGTTGTAACGTACCGTCGGTAATGGGTGCACGTACGCTTGATGCACCGCGTGAACGTCtgat                                                                      >  2:11083/1‑140 (MQ=255)                                                                gcggcgTTTGTGATGGACCGTCTGATGCAGGCGCTGGGCTTGCCGGGGAAATCCTTTGTGCCGCTGATCGTCGGCTTCGGTTGTAACGTACCGTCGGTAATGGGTGCACGTACGCTTGATGCACCGCGTGAACGTCtgat                                                                      <  1:235627/140‑1 (MQ=255)                                                                 cggcgTTTGTGATGGACCGTCTGATGCAGGCGCTGGGCTTGCCGGGGAAATCCTTTGTGCCGCTGATCGTCGGCTTCGGTTGTAACGTACCGTCGGTAATGGGTGCACGTACGCTTGATGCACCGCGTGAACGTCtgatg                                                                     >  2:872700/1‑140 (MQ=255)                                                                   gcgTTTGTGATGGACCGTCTGATGCAGGCGCTGGGCTTGCCGGGGAAATCCTTTGTGCCGCTGATCGTCGGCTTCGGTTGTAACGTACCGTCGGTAATGGGTGCACGTACGCTTGATGCACCGCGTGAACGTCTGATGAc                                                                   <  2:776348/140‑1 (MQ=255)                                                                        tgtgATGGACCGTCTGATGCAGGCGCTGGGCTTGCCGGGGAAATCCTTTGTGCCGCTGATCGTCGGCTTCGGTTGTAACGTACCGTCGGTAATGGGTGCACGTACGCTTGATGCACCGCGTGAACGTCTGATGACcatca                                                              >  1:20732/1‑140 (MQ=255)                                                                          tgATGGACCGTCTGATGCAGGCGCTGGGCTTGCCGGGGAAATCCTTTGTGCCGCTGATCGTCGGCTTCGGTTGTAACGTACCGTCGGTAATGGGTGCACGTACGCTTGATGCACCGCGTGAACGTCTGATGACCATCatg                                                            <  1:735471/140‑1 (MQ=255)                                                                           gATGGACCGTCTGATGCAGGCGCTGGGCTTGCCGGGGAAATCCTTTGTGCCGCTGATCGTCGGCTTCGGTTGTAACGTACCGTCGGTAATGGGTGCACGTACGCTTGATGCACCGCGTGAACGTCTGATGACCATCatga                                                           <  1:652027/140‑1 (MQ=255)                                                                              ggACCGTCTGATGCAGGCGCTGGGCTTGCCGGGGAAATCCTTTGTGCCGCTGATCGTCGGCTTCGGTTGTAACGTACCGTCGGTAATGGGTGCACGTACGCTTGATGCACCGCGTGAACGTCTGATGACCATCATGATgg                                                        <  2:705579/140‑1 (MQ=255)                                                                              ggACCGTCTGATGCAGGCGCTGGGCTTGCCGGGGAAATCCTTTGTGCCGCTGATCGTCGGCTTCGGTTGTAACGTACCGTCGGTAATGGGTGCACGTACGCTTGATGCACCGCGTGAACGTCTGATGACCATCATGATgg                                                        <  2:908785/140‑1 (MQ=255)                                                                              ggACCGTCTGATGCAGGCGCTGGGCTTGCCGGGGAAATCCTTTGTGCCGCTGATCGTCGGCTTCGGTTGTAACGTACCGTCGGTAATGGGTGCACGTACGCTTGATGCACCGCGTGAACGTCTGATGACCATCATGATgg                                                        <  2:819005/140‑1 (MQ=255)                                                                               gACCGTCTGATGCAGGCGCTGGGCTTGCCGGGGAAATCCTTTGTGCCGCTGATCGTCGGCTTCGGTTGTAACGTACCGTCGGTAATGGGTGCACGTACGCTTGATGCACCGCGTGAACGTCTGATGACCATCATGATGGc                                                       <  2:948370/140‑1 (MQ=255)                                                                               gACCGTCTGATGCAGGCGCTGGGCTTGCCGGGGAAATCCTTTGTGCCGCTGATCGTCGGCTTCGGTTGTAACGTACCGTCGGTAATGGGTGCACGTACGCTTGATGCACCGCGTGAACGTCTGATGACCATCATGATGGc                                                       <  1:872700/140‑1 (MQ=255)                                                                               gACCGTCTGATGCAGGCGCTGGGCTTGCCGGGGAAATCCTTTGTGCCGCTGATCGTCGGCTTCGGTTGTAACGTACCGTCGGTAATGGGTGCACGTACGCTTGATGCACCGCGTGAACGTCTGATGACCATCATGATGGc                                                       <  2:183656/140‑1 (MQ=255)                                                                               gACCGTCTGATGCAGGCGCTGGGCTTGCCGGGGAAATCCTTTGTGCCGCTGATCGTCGGCTTCGGTTGTAACGTACCGTCGGTAATGGGTGCACGTACGCTTGATGCACCGCGTGAACGTCTGATGACCATCATGATGGc                                                       <  2:189050/140‑1 (MQ=255)                                                                                aCCGTCTGATGCAGGCGCTGGGCTTGCCGGGGAAATCCTTTGTGCCGCTGATCGTCGGCTTCGGTTGTAACGTACCGTCGGTAATGGGTGCACGTACGCTTGATGCACCGCGTGAACGTCTGATGACCATCATGATGGCa                                                      >  2:819065/1‑140 (MQ=255)                                                                                 ccGTCTGATGCAGGCGCTGGGCTTGCCGGGGAAATCCTTTGTGCCGCTGATCGTCGGCTTCGGTTGTAACGTACCGTCGGTAATGGGTGCACGTACGCTTGATGCACCGCGTGAACGTCTGATGACCATCATGATGGCAc                                                     <  2:73425/140‑1 (MQ=255)                                                                                 ccGTCTGATGCAGGCGCTGGGCTTGCCGGGGAAATCCTTTGTGCCGCTGATCGTCGGCTTCGGTTGTAACGTACCGTCGGTAATGGGTACACGTACGCTTGATGCACCGCGTGAACGTCTGATGACCATCATGATGGCAc                                                     <  2:347894/140‑1 (MQ=255)                                                                                   gTCTGATGCAGGCGCTGGGCTTGCCGGGGAAATCCTTTGTGCCGCTGATCGTCGGCTTCGGTTGTAACGTACCGTCGGTAATGGGTGCACGTACGCTTGATGCACCGCGTGAACGTCTGATGACCATCATGATGGCACCg                                                   <  2:161699/140‑1 (MQ=255)                                                                                      tGATGCAGGCGCTGGGCTTGCCGGGGAAATCCTTTGTGCCGCTGATCGTCGGCTTCGGTTGTAACGTACCGTCGGTAATGGGTGCACGAACGCTTGGTGCACCGCGTGAACGTCTGATGACCATCATGATGGCACCGttt                                                >  2:900623/1‑140 (MQ=255)                                                                                      tGATGCAGGCGCTGGGCTTGCCGGGGAAATCCTTTGTGCCGCTGATCGTCGGCTTCGGTTGTAACGTACCGTCGGTAATGGGTGCACGAACGCTTGATGCACCGCGTGAACGTCTGATGACCATCATGATGGCACCGttt                                                <  1:900623/140‑1 (MQ=255)                                                                                              gcgcTGGGCTTGCCGGGGAAATCCTTTGTGCCGCTGATCGTCGGCTTCGGTTGTAACGTACCGTCGGTAATGGGTGCACGTACGCTTGATGCACCGCGTGAACGTCTGATGACCATCATGATGGCACCGTTTATGTCCTg                                        >  1:686136/1‑140 (MQ=255)                                                                                              gcgcTGGGCTTGCCGGGGAAATCCTTTGTGCCGCTGATCGTCGGCTTCGGTTGTAACGTACCGTCGGTAATGGGTGCACGTACGCTTGATGCACCGCGTGAACGTCTGATGACCATCATGATGGCACCGTTTATGTCCTg                                        >  1:328601/1‑140 (MQ=255)                                                                                               cgcTGGGCTTGCCGGGGAAATCCTTTGTGCCGCTGATCGTCGGCTTCGGTTGTAACGTACCGTCGGTAATGGGTGCACGTACGCTTGATGCACCGCGTGAACGTCTGATGACCATCATGATGGCACCGTTTATGTCCTgc                                       >  2:754214/1‑140 (MQ=255)                                                                                               cgcTGGGCTTGCCGGGGAAATCCTTTGTGCCGCTGATCGTCGGCTTCGGTTGTAACGTACCGTCGGTAATGGGTGCACGTACGCTTGATGCACCGCGTGAACGTCTGATGACCATCATGATGGCACCGTTTATGTCCTgc                                       >  2:500051/1‑140 (MQ=255)                                                                                                   gggCTTGCCGGGGAAATCCTTTGTGCCGCTGATCGTCGGCTTCGGTTGTAACGTACCGTCGGTAATGGGTGCACGTACGCTTGATGCACCGCGTGAACGTCTGATGACCATCATGATGGCACCGTTTATGTCCTgcggcg                                   >  2:725808/1‑140 (MQ=255)                                                                                                   gggCTTGCCGGGGAAATCCTTTGTGCCGCTGATCGTCGGCTTCGGTTGTAACGTACCGTCGGTAATGGGTGCACGTACGCTTGATGCACCGCGTGAACGTCTGATGACCATCATGATGGCACCGTTTATGTCCTgcggcg                                   >  2:467770/1‑140 (MQ=255)                                                                                                         gCCGGGGAAATCCTTTGTGCCGCTGATCGTCGGCTTCGGTTGTAACGTACCGTCGGTAATGGGTGCACGTACGCTTGATGCACCGCGTGAACGTCTGATGACCATCATGATGGCACCGTTTATGTCCTGCGGCGCGCGTc                             >  2:414647/1‑140 (MQ=255)                                                                                                                   tCCTTTGTGCCGCTGATCGTCGGCTTCGGTTGTAACGTACCGTCGGTAATGGGTGCACGTACGCTTGATGCACCGCGTGAACGTCTGATGACCATCATGATGGCACCGTTTATGTCCTGCGGCGCGCGTCTGGCTATCtt                   >  2:181590/1‑140 (MQ=255)                                                                                                                       ttGTGCCGCTGATCGTCGGCTTCGGTTGTAACGTACCGTCGGTAATGGGTGCATGTACGCTTGATGCACCGCGTGAACGTCTGATGACCATCATGATGGCACCGTTTATGTCCTGCGGCGCGCGTCTGGCTATCTTCGCa               <  2:844545/140‑1 (MQ=255)                                                                                                                          tgCCGCTGATCGTCGGCTTCGGTTGTAACGTACCGTCGGTAATGGGTGCACGTACGCTTGATGCACCGCGTGAACGTCTGATGACCATCATGATGGCACCGTTTATGTCCTGCGGCGCGCGTCTGGCTATCTTCGCAGTa            >  1:897405/1‑140 (MQ=255)                                                                                                                               cTGATCGTCGGCTTCGGTTGTAACGTACCGTCGGTAATGGGTGCACGTACGCTTGATGCACCGCGTGAACGTCTGATGACCATCATGATGGCACCGTTTATGTCCTGCGGCGCGCGTCTGGCTATCTTCGCa               <  1:51787/132‑1 (MQ=255)                                                                                                                               cTGATCGTCGGCTTCGGTTGTAACGTACCGTCGGTAATGGGTGCACGTACGCTTGATGCACCGCGTGAACGTCTGATGACCATCATGATGGCACCGTTTATGTCCTGCGGCGCGCGTCTGGCTATCTTCGCa               >  2:51787/1‑132 (MQ=255)                                                                                                                                    cgtcgGCTTCGGTTGTAACGTACCGTCGGTAATGGGTGCACGTACGCTTGATGCACCGCGTGAACGTCTGATGACCATCATGATGGCACCGTTTATGTCCTGCGGCGCGCGTCTGGCTATCTTCGCAGTATTCGCGGCTg  >  2:313907/1‑140 (MQ=255)                                                                                                                                          |                                                                                                                                      CCGCAGATTGGCATGATGTACCTGTTCCTCTCCTTCCTTGAGGACTCCGGGTATATGGCGCGTGCGGCGTTTGTGATGGACCGTCTGATGCAGGCGCTGGGCTTGCCGGGGAAATCCTTTGTGCCGCTGATCGTCGGTTTCGGTTGTAACGTACCGTCGGTAATGGGTGCACGTACGCTTGATGCACCGCGTGAACGTCTGATGACCATCATGATGGCACCGTTTATGTCCTGCGGCGCGCGTCTGGCTATCTTCGCAGTATTCGCGGCTG  >  NC_000913/3541469‑3541739

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑