Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	436,703	A→G	T39A (ACG→GCG)	pgpA →	phosphatidylglycerophosphatase A

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	436,703	0	A	G	100.0%	191.9 / NA	53	T39A (ACG→GCG)	pgpA	phosphatidylglycerophosphatase A
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (30/23);  total (30/23)

ACTGGAAAGGATATGACCATTTTGCCACGCCATAAAGATGTCGCGAAAAGTCGCCTGAAGATGAGTAATCCGTGGCATCTACTTGCTGTCGGATTCGGAAGTGGATTAAGCCCGATCGTTCCTGGGACGATGGGCTCGCTGGCAGCGATTCCGTTCTGGTATCTGATGACCTTTTTGCCCTGGCAGCTCTACTCGCTGGTGGTGATGCTGGGGATCTGTATCGGCGTCTATCTTTGTCATCAAACGGCGAAAGACATGGGTG  >  NC_000913/436577‑436838                                                                                                                               |                                                                                                                                        aCTGGAAAGGATATGACCATTTTGCCACGCCATAAAGATGTCGCGAAAAGTCGCCTGAAGATGAGTAATCCGTGGCATCTACTTGCTGTCGGATTCGGAAGTGGATTAAGCCCGATCGTTCCTGGGGCGATGGGCTCGCt                                                                                                                            >  1:672059/1‑140 (MQ=255)     gAAAGGATATGACCATTTTGCCACGCCATAAAGATGTCGCGAAAAGTCGCCTGAAGATGAGTAATCCGTGGCATCTACTTGCTGTCGGATTCGGAAGTGGATTAAGCCCGATCGTTCCTGGGGCGATGGGCTCGCTGGCa                                                                                                                        >  1:493350/1‑140 (MQ=255)          gATATGACCATTTTGCCACGCCATAAAGATGTCGCGAAAAGTCGCCTGAAGATGAGTAATCCGTGGCATCTACTTGCTGTCGGATTCGGAAGTGGATTAAGCCCGATCGTTCCTGGGGCGATGGGCTCGCTGGCAGCGAt                                                                                                                   >  2:618648/1‑140 (MQ=255)             atGACCATTTTGCCACGCCATAAAGATGTCGCGAAAAGTCGCCTGAAGATGAGTAATCCGTGGCATCTACTTGCTGTCGGATTCGGAAGTGGATTAAGCCCGATCGTTCCTGGGGCGATGGGCTCGCTGGCAGCGATTcc                                                                                                                >  1:861965/1‑140 (MQ=255)             atGACCATTTTGCCACGCCATAAAGATGTCGCGAAAAGTCGCCTGAAGATGAGTAATCCGTGGCATCTACTTGCTGTCGGATTCGGAAGTGGATTAAGCCCGATCGTTCCTGGGGCGATGGGCTCGCTGGCAGCGATTcc                                                                                                                <  1:965427/140‑1 (MQ=255)               gACCATTTTGCCACGCCATAAAGATGTCGCGAAAAGTCGCCTGAAGATGAGTAATCCGTGGCATCTACTTGCTGTCGGATTCGGAAGTGGATTAAGCCCGATCGTTCCTGGGGCGATGGGCTCGCTGGCAGCGATTCCGt                                                                                                              >  2:965907/1‑140 (MQ=255)                    ttttGCCACGCCATAAAGATGTCGCGAAAAGTCGCCTGAAGATGAGTAATCCGTGGCATCTACTTGCTGTCGGATTCGGAAGTGGATTAAGCCCGATCGTTCCTGGGGCGATGGGCTCGCTGGCAGCGATTCCGTTCTgg                                                                                                         >  1:168302/1‑140 (MQ=255)                    ttttGCCACGCCATAAAGATGTCGCGAAAAGTCGCCTGAAGATGAGTAATCCGTGGCATCTACTTGCTGTCGGATTCGGAAGTGGATTAAGCCCGATCGTTCCTGGGGCGATGGGCTCGCTGGCAGCGATTCCGTTCTgg                                                                                                         >  1:795901/1‑140 (MQ=255)                     tttGCCACGCCATAAAGATGTCGCGAAAAGTCGCCTGAAGATGAGTAATCCGTGGCATCTACTTGCTGTCGGATTCGGAAGTGGATTAAGCCCGATCGTTCCTGGGGCGATGGGCTCGCTGGCAGCGATTCCGTTCTGGt                                                                                                        <  2:493350/140‑1 (MQ=255)                     tttGCCACGCCATAAAGATGTCGCGAAAAGTCGCCTGAAGATGAGTAATCCGTGGCATCTACTTGCTGTCGGATTCGGAAGTGGATTAAGCCCGATCGTTCCTGGGGCGATGGGCTCGCTGGCAGCGATTCCGTTCTGGt                                                                                                        >  2:840285/1‑140 (MQ=255)                                  aaaGATGTCGCGAAAAGTCGCCTGAAGATGAGTAATCCGTGGCATCTACTTGCTGTCGGATTCGGAAGTGGATTAAGCCCGATCGTTCCTGGGGCGATGGGCTCGCTGGCAGCGATTCCGTTCTGGTATCTGATGACCtt                                                                                           <  2:625848/140‑1 (MQ=255)                                   aaGATGTCGCGAAAAGTCGCCTGAAGATGAGTAATACGTGGCATCTACTTGCTGTCGGATTCGGAAGTGGATTAAGCCCGTTCGTTCCTGGGGCGATGGGCTCGCTGGCAGCGATTCCGTTCTGGTATCTGATGACCttt                                                                                          <  2:672059/140‑1 (MQ=255)                                       tGTCGCGAAAAGTCGCCTGAAGATGAGTAATCCGTGGCATCTACTTGCTGTCGGATTCGGAAGTGGATTAAGCCCGATCGTTCCTGGGGCGATGGGCTCGCTGGCAGCGATTCCGTTCTGGTATCTGATGACCTTTTTGc                                                                                      <  2:795901/140‑1 (MQ=255)                                       tGTCGCGAAAAGTCGCCTGAAGATGAGTAATCCGTGGCATCTACTTGCTGTCGGATTCGGAAGTGGATTAAGCCCGATCGTTCCTGGGGCGATGGGCTCGCTGGCAGCGATTCCGTTCTGGTATCTGATGACCTTTTTGc                                                                                      <  1:618648/140‑1 (MQ=255)                                         tCGCGAAAAGTCGCCTGAAGATGAGTAATCCGTGGCATCTACTTGCTGTCGGATTCGGAAGTGGATTAAGCCCGATCGTTCCTGGGGCGATGGGCTCGCTGGCAGCGATTCCGTTCTGGTATCTGATGACCTTTTTGccc                                                                                    >  2:183005/1‑140 (MQ=255)                                         tCGCGAAAAGTCGCCTGAAGATGAGTAATCCGTGGCATCTACTTGCTGTCGGATTCGGAAGTGGATTAAGCCCGATCGTTCCTGGGGCGATGGGCTCGCTGGCAGCGATTCCGTTCTGGTATCTGATGACCTTTTTGccc                                                                                    >  1:668807/1‑140 (MQ=255)                                              aaaaGTCGCCTGAAGATGAGTAATCCGTGGCATCTACTTGCTGTCGGATTCGGAAGTGGATTAAGCCCGATCGTTCCTGGGGCGATGGGCTCGCTGGCAGCGATTCCGTTCTGGTATCTGATGACCTTTTTGCCCTGGCa                                                                               <  1:618119/140‑1 (MQ=255)                                                   tCGCCTGAAGATGAGTAATCCGTGGCATCTACTTGCTGTCGGATTCGGAAGTGGATTAAGCCCGATCGTTCCTGGGGCGATGGGCTCGCTGGCAGCGATTCCGTTCTGGTATCTGATGACCTTTTTGCCCTGGCAGctct                                                                          >  2:698512/1‑140 (MQ=255)                                                    cGCCTGAAGATGAGTAATCCGTGGCATCTACTTGCTGTCGGATTCGGAAGTGGATTAAGCCCGATCGTTCCTGGGGCGATGGGCTCGCTGGCAGCGATTCCGTTCTGGTATCTGATGACCTTTTTGCCCTGGCAGCTCTa                                                                         <  1:965907/140‑1 (MQ=255)                                                         gAAGATGAGTAATCCGTGGCATCTACTTGCTGTCGGATTCGGAAGTGGATTAAGCCCGATCGTTCCTGGGGCGATGGGCTCGCTGGCAGCGATTCCGTTCTGGTATCTGATGACCTTTTTGCCCTGGCAGCTCTACTCGc                                                                    <  1:183005/140‑1 (MQ=255)                                                                aGTAATCCGTGGCATCTACTTGCTGTCGGATTCGGAAGTGGATTAAGCCCGATCGTTCCTGGGGCGATGGGCTCGCTGGCAGCGATTCCGTTCTGGTATCTGATGACCTTTTTGCCCTGGCAGCTCTACTCGCTggtggt                                                             <  1:698512/140‑1 (MQ=255)                                                                 gTAATCCGTGGCATCTACTTGCTGTCGGATTCGGAAGTGGATTAAGCCCGATCGTTCCTGGGGCGATGGGCTCGCTGGCAGCGATTCCGTTCTGGTATCTGATGACCTTTTTGCCCTGGCAGCTCTACTCGCTGgtggtg                                                            >  1:47959/1‑140 (MQ=255)                                                                      ccGTGGCATCTACTTGCTGTCGGATTCGGAAGTGGATTAAGCCCGATCGTTCCTGGGGCGATGGGCTCGCTGGCAGCGATTCCGTTCTGGTATCTGATGACCTTTTTGCCCTGGCAGCTCTACTCGCTGGTGGTGATGCt                                                       >  1:623312/1‑140 (MQ=255)                                                                         tGGCATCTACTTGCTGTCGGATTCGGAAGTGGATTAAGCCCGATCGTTCCTGGGGCGATGGGCTCGCTGGCAGCGATTCCGTTCTGGTATCTGATGACCTTTTTGCCCTGGCAGCTCTACTCGCTGGTGGTGATGCTggg                                                    >  1:238596/1‑140 (MQ=255)                                                                         tGGCATCTACTTGCTGTCGGATTCGGAAGTGGATTAAGCCCGATCGTTCCTGGGGCGATGGGCTCGCTGGCAGCGATTCCGTTCTGGTATCTGATGACCTTTTTGCCCTGGCAGCTCTACTCGCTGGTGGTGATGCTggg                                                    >  2:551944/1‑140 (MQ=255)                                                                          ggCATCTACTTGCTGTCGGATTCGGAAGTGGATTAAGCCCGATCGTTCCTGGGGCGATGGGCTCGCTGGCAGCGATTCCGTTCTGGTATCTGATGACCTTTTTGCCCTGGCAGCTCTACTCGCTGGTGGTGATGCTgggg                                                   >  2:583732/1‑140 (MQ=255)                                                                          ggCATCTACTTGCTGTCGGATTCGGAAGTGGATTAAGCCCGATCGTTCCTGGGGCGATGGGCTCGCTGGCAGCGATTCCGTTCTGGTATCTGATGACCTTTTTGCCCTGGCAGCTCTACTCGCTGGTGGTGATGCTgggg                                                   >  2:48435/1‑140 (MQ=255)                                                                          ggCATCTACTTGCTGTCGGATTCGGAAGTGGATTAAGCCCGATCGTTCCTGGGGCGATGGGCTCGCTGGCAGCGATTCCGTTCTGGTATCTGATGACCTTTTTGCCCTGGCAGCTCTACTCGCTGGTGGTGATGCTgggg                                                   >  2:46151/1‑140 (MQ=255)                                                                            cATCTACTTGCTGTCGGATTCGGAAGTGGATTAAGCCCGATCGTTCCTGGGGCGATGGGCTCGCTGGCAGCGATTCCGTTCTGGTATCTGATGACCTTTTTGCCCTGGCAGCTCTACTCGCTGGTGGTGATGCTGGGGAt                                                 >  1:862636/1‑140 (MQ=255)                                                                                  cTTGCTGTCGGATTCGGAAGTGGATTAAGCCCGATCGTTCCTGGGGCGATGGGCTCGCTGGCAGCGATTCCGTTCTGGTATCTGATGACCTTTTTGCCCTGGCAGCTCTACTCGCTGGTGGTGATGCTGGGGATCTGTAt                                           <  1:551944/140‑1 (MQ=255)                                                                                   ttGCTGTCGGATTCGGAAGTGGATTAAGCCCGATCGTTCCTGGGGCGATGGGCTCGCTGGCAGCGATTCCGTTCTGGTATCTGATGACCTTTTTGCCCTGGCAGCTCTACTCGCTGGTGGTGATGCTGGGGATCTGTATc                                          <  1:583732/140‑1 (MQ=255)                                                                                   ttGCTGTCGGATTCGGAAGTGGATTAAGCCCGATCGTTCCTGGGGCGATGGGCTCGCTGGCAGCGATTCCGTTCTGGTATCTGATGACCTTTTTGCCCTGGCAGCTCTACTCGCTGGTGGTGATGCTGGGGATCTGTATc                                          <  1:48435/140‑1 (MQ=255)                                                                                      cTGTCGGATTCGGAAGTGGATTAAGCCCGATCGTTCCTGGGGCGATGGGCTCGCTGGCAGCGATTCCGTTCTGGTATCTGATGACCTTTTTGCCCTGGCAGCTCTACTCGCTGGTGGTGATGCTGGGGATCTGTATCGGc                                       <  1:840285/140‑1 (MQ=255)                                                                                         tCGGATTCGGAAGTGGATTAAGCCCGATCGTTCCTGGGGCGATGGGCTCGCTGGCAGCGATTCCGTTCTGGTATCTGATGACCTTTTTGCCCTGGCAGCTCTACTCGCTGGTGGTGATGCTGGGGATCTGTATCGGCGTc                                    >  1:342618/1‑140 (MQ=255)                                                                                         tCGGATTCGGAAGTGGATTAAGCCCGATCGTTCCTGGGGCGATGGGCTCGCTGGCAGCGATTCCGTTCTGGTATCTGATGACCTTTTTGCCCTGGCAGCTCTACTCGCTGGTGGTGATGCTGGGGATCTGTATCGGCGTc                                    >  1:175203/1‑140 (MQ=255)                                                                                          cGGATTCGGAAGTGGATTAAGCCCGATCGTTCCTGGGGCGATGGGCTCGCTGGCAGCGATTCCGTTCTGGTATCTGATGACCTTTTTGCCCTGGCAGCTCTACTCGCTGGTGGTGATGCTGGGGATCTGTATCGGCGTCt                                   >  1:731820/1‑140 (MQ=255)                                                                                            gATTCGGAAGTGGATTAAGCCCGATCGTTCCTGGGGCGATGGGCTCGCTGGCAGCGATTCCGTTCTGGTATCTGATGACCTTTTTGCCCTGGCAGCTCTACTCGCTGGTGGTGATGCTGGGGATCTGTATCGGCGTCTAt                                 >  2:173381/1‑140 (MQ=255)                                                                                              ttCGGAAGTGGATTAAGCCCGATCGTTCCTGGGGCGATGGGCTCGCTGGCAGCGATTCCGTTCTGGTATCTGATGACCTTTTTGCCCTGGCAGCTCTACTCGCTGGTGGTGATGCTGGGGATCTGTATCGGCGTCTATCt                               >  1:60859/1‑140 (MQ=255)                                                                                              ttCGGAAGTGGATTAAGCCCGATCGTTCCTGGGGCGATGGGCTCGCTGGCAGCGATTCCGTTCTGGTATCTGATGACCTTTTTGCCCTGGCAGCTCTACTCGCTGGTGGTGATGCTGGGGATCTGTATCGGCGTCTATCt                               >  2:70206/1‑140 (MQ=255)                                                                                                        gATTAAGCCCGATCGTTCCTGGGGCGATGGGCTCGCTGGCAGCGATTCCGTTCTGGTATCTGATGACCTTTTTGCCCTGGCAGCTCTACTCGCTGGTGGTGATGCTGGGGATCTGTATCGGCGTCTATCTTTGTCATCaa                     >  2:39847/1‑140 (MQ=255)                                                                                                         aTTAAGCCCGATCGTTCCTGGGGCGATGGGCTCGCTGGCAGCGATTCCGTTCTGGTATCTGATGACCTTTTTGCCCTGGCAGCTCTACTCGCTGGTGGTGATGCTGGGGATCTGTATCGGCGTCTATCTTTGTCATCaaa                    <  2:238596/140‑1 (MQ=255)                                                                                                            aaGCCCGATCGTTCCTGGGGCGATGGGCTCGCTGGCAGCGATTCCGTTCTGGTATCTGATGACCTTTTTGCCCTGGCAGCTCTACTCGCTGGTGGTGATGCTGGGGATCTGTATCGGCGTCTATCTTTGTCATCAAACgg                 <  1:46151/140‑1 (MQ=255)                                                                                                              gCCCGATCGTTCCTGGGGCGATGGGCTCGCTGGCAGCGATTCCGTTCTGGTATCTGATGACCTTTTTGCCCTGGCAGCTCTACTCGCTGGTGGTGATGCTGGGGATCTGTATCGGCGTCTATCTTTGTCATCAAACGGCg               <  2:502463/140‑1 (MQ=255)                                                                                                              gCCCGATCGTTCCTGGGGCGATGGGCTCGCTGGCAGCGATTCCGTTCTGGTATCTGATGACCTTTTTGCCCTGGCAGCTCTACTCGCTGGTGGTGATGCTGGGGATCTGTATCGGCGTCTATCTTTGTCATCAAACGGCg               <  2:175203/140‑1 (MQ=255)                                                                                                              gCCCGATCGTTCCTGGGGCGATGGGCTCGCTGGCAGCGATTCCGTTCTGGTATCTGATGACCTTTTTGCCCTGGCAGCTCTACTCGCTGGTGGTGATGCTGGGGATCTGTATCGGCGTCTATCTTTGTCATCAAACGGCg               <  2:168302/140‑1 (MQ=255)                                                                                                                ccGATCGTTCCTGGGGCGATGGGCTCACTGGCAGCGATTCCGTTCTGGTATCTGATGACCTTTTTGCCCTGGCAGCTCTACTCGCTGGTGGTGATGCTGGGGATCTGTATCGGCGTCTATCTTTGTCATCAAACGGCGaa             >  2:254126/1‑140 (MQ=255)                                                                                                                  gATCGTTCCTGGGGCGATGGGCTCGCTGGCAGCGATTCCGTTCTGGTATCTGATGACCTTTTTGCCCTGGCAGCTCTACTCGCTGGTGGTGATGCTGGGGATCTGTATCGGCGTCTATCTTTGTCATCAAACGGCGAAAg           >  1:11114/1‑140 (MQ=255)                                                                                                                   aTCGTTCCTGGGGCGATGGGCTCGCTGGCAGCGATTCCGTTCTGGTATCTGATGACCTTTTTGCCCTGGCAGCTCTACTCGCTGGTGGTGATGCTGGGGATCTGTATCGGCGTCTATCTTTGTCATCAAACgg                 >  1:165781/1‑133 (MQ=255)                                                                                                                   aTCGTTCCTGGGGCGATGGGCTCGCTGGCAGCGATTCCGTTCTGGTATCTGATGACCTTTTTGCCCTGGCAGCTCTACTCGCTGGTGGTGATGCTGGGGATCTGTATCGGCGTCTATCTTTGTCATCAAACgg                 <  2:165781/133‑1 (MQ=255)                                                                                                                   aTCGTTCCTGGGGCGATGGGCTCGCTGGCAGCGATTCCGTTCTGGTATCTGATGACCTTTTTGCCCTGGCAGCTCTACTCGCTGGTGGTGATGCTGGGGATCTGTATCGGCGTCTATCTTTGTCATCAAACGGCGAAAGa          >  2:60349/1‑140 (MQ=255)                                                                                                                        tCCTGGGGCGATGGGCTCGCTGGCAGCGATTCCGTTCTGGTATCTGATGACCTTTTTGCCCTGGCAGCTCTACTCGCTGGTGGTGATGCTGGGGATCTGTATCGGCGTCTATCTTTGTCATCAAACGGCGAAAGACATgg     <  2:60859/140‑1 (MQ=255)                                                                                                                        tCCTGGGGCGATGGGCTCGCTGGCAGCGATTCCGTTCTGGTATCTGATGACCTTTTTGCCCTGGCAGCTCTACTCGCTGGTGGTGATGCTGGGGATCTGTATCGGCGTCTATCTTTGTCATCAAACGGCGAAAGACATgg     <  2:342618/140‑1 (MQ=255)                                                                                                                           tGGGGCGATGGGCTCGCTGGCAGCGATTCCGTTCTGGTATCTGATGACCTTTTTGCCCTGGCAGCTCTACTCGCTGGTGGTGATGCTGGGGATCTGTATCGGCGTCTATCTTTGTCATCAAACGGCGAAAGACATGGgtg  <  2:862636/140‑1 (MQ=255)                                                                                                                               |                                                                                                                                        ACTGGAAAGGATATGACCATTTTGCCACGCCATAAAGATGTCGCGAAAAGTCGCCTGAAGATGAGTAATCCGTGGCATCTACTTGCTGTCGGATTCGGAAGTGGATTAAGCCCGATCGTTCCTGGGACGATGGGCTCGCTGGCAGCGATTCCGTTCTGGTATCTGATGACCTTTTTGCCCTGGCAGCTCTACTCGCTGGTGGTGATGCTGGGGATCTGTATCGGCGTCTATCTTTGTCATCAAACGGCGAAAGACATGGGTG  >  NC_000913/436577‑436838

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑