Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,757,182	C→T	A216T (GCA→ACA)	yiaY ←	L‑threonine dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,757,182	0	C	T	100.0%	282.6 / NA	82	A216T (GCA→ACA)	yiaY	L‑threonine dehydrogenase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (34/48);  total (34/48)

CCCAGAGAAGCATTATTGAACGCCATACCGGCGAGGAACTGGGCATAAGCCATTGCTTCACGCGCTTTCGCATTACTGCCATCTTCAACGGCTAACGGCAGGTTTTCGGCAATCATGGTCACGGCTTTCAGTGCACAAGCGTCAGTGATCGGCGTGGCGGCAATAGAAACATATGCTTCGATAGCGTGCGTTAAGGCATCCATACCCGTTGCGGCGGTCAGTGACTTCGGCATACCAATCATCAGAGAGGAGTCATTGACAGAAAGCAG  >  NC_000913/3757049‑3757317                                                                                                                                      |                                                                                                                                        cccAGAGAAGCATTATTGAACGCCATACCGGCGAGGAACTGGGCATAAGCCATTGCTTCACGCGCTTTCGCATTACTGCCATCTTCAACGGCTAACGGCAGGTTTTCGGCAATCATGGTCACGGCTTTCAGTGTACAAGc                                                                                                                                   >  1:600808/1‑140 (MQ=255) cccAGAGAAGCATTATTGAACGCCATACCGGCGAGGAACTGGGCATAAGCCATTGCTTCACGCGCTTTCGCATTACTGCCATCTTCAACGGCTAACGGCAGGTTTTCGGCAATCATGGTCACGGCTTTCAGTGTACAAGc                                                                                                                                   >  2:388488/1‑140 (MQ=255)    agagAAGCATTATTGAACGCCATACCGGCGAGGAACTGGGCATAAGCCATTGCTTCACGCGCTTTCGCATTACTGCCATCTTCAACGGCTAACGGCAGGTTTTCGGCAATCATGGTCACGGCTTTCAGTGtaca                                                                                                                                      <  1:227495/134‑1 (MQ=255)    agagAAGCATTATTGAACGCCATACCGGCGAGGAACTGGGCATAAGCCATTGCTTCACGCGCTTTCGCATTACTGCCATCTTCAACGGCTAACGGCAGGTTTTCGGCAATCATGGTCACGGCTTTCAGTGtaca                                                                                                                                      >  2:227495/1‑134 (MQ=255)    agagAAGCATTATTGAACGCCATACCGGCGAGGAACTGGGCATAAGCCATTGCTTCACGCGCTTTCGCATTACTGCCATCTTCAACGGCTAACGGCAGGTTTTCGGCAATCATGGTCACGGCTTTCAGTGTACAAGCGTc                                                                                                                                >  1:594181/1‑140 (MQ=255)     gagaAGCATTATTGAACGCCATACCGGCGAGGAACTGGGCATAAGCCATTGCTTCACGCGCTTTCGCATTACTGCCATCTTCAACGGCTAACGGCAGGTTTTCGGCAATCATGGTCACGGCTTTCAGTGTACAAGCGTCa                                                                                                                               <  1:388488/140‑1 (MQ=255)      agaAGCATTATTGAACGCCATACCGGCGAGGAACTGGGCATAAGCCATTGCTTCACGCGCTTTCGCATTACTGCCATCTTCAACGGCTAACGGCAGGTTTTCGGCAATCATGGTCACGGCTTTTAGTGTACAAGCGTCAg                                                                                                                              >  2:658981/1‑140 (MQ=255)      agaAGCATTATTGAACGCCATACCGGCGAGGAACTGGGCATAAGCCATTGCTTCACGCGCTTTCGCATTACTGCCATCTTCAACGGCTAACGACAGGTTTTCGGCAATCATGGTCACGGCTTTCAGTGTACAAGCGTCAg                                                                                                                              >  2:328589/1‑140 (MQ=255)           cATTATTGAACGCCATACCGGCGAGGAACTGGGCATAAGCCATTGCTTCACGCGCTTTCGCATTACTGCCATCTTCAACGGCTAACGGCAGGTTTTCGGCAATCATGGTCACGGCTTTCAGTGTACAAGCGTCAGTGATc                                                                                                                         >  1:361897/1‑140 (MQ=255)            attattGAACGCCATACCGGCGAGGAACTGGGCATAAGCCATTGCTTCACGCGCTTTCGCATTACTGCCATCTTCAACGGCTAACGGCAGGTTTTCGGCAATCATGGTCACGGCTTTCAGTGTACAAGCGTCAGTGATCg                                                                                                                        >  2:832887/1‑140 (MQ=255)                ttGAACGCCATACCGGCGAGGAACTGGGCATAAGCCATTGCTTCACGCGCTTTCGCATTACTGCCATCTTCAACGGCTAACGGCAGGTTTTCGGCAATCATGGTCACGGCTTTCAGTGTACAAGCGTCAGTGATCGGCGt                                                                                                                    >  1:123040/1‑140 (MQ=255)                   aaCGCCATACCGGCGAGGAACTGGGCATAAGCCATTGCTTCACGCGCTTTCGCATTACTGCCATCTTCAACGGCTAACGGCAGGTTTTCGGCAATCATGGTCACGGCTTTCAGTGTACAAGCGTCAGTGATCGGCGTggc                                                                                                                 <  1:696623/140‑1 (MQ=255)                   aaCGCCATACCGGCGAGGAACTGGGCATAAGCCATTGCTTCACGCGCTTTCGCATTACTGCCATCTTCAACGGCTAACGGCAGGTTTTCGGCAATCATGGTCACGGCTTTCAGTGTACAAGCGTCAGTGATCGGCGTggc                                                                                                                 <  2:124518/140‑1 (MQ=255)                   aaCGCCATACCGGCGAGGAACTGGGCATAAGCCATTGCTTCACGCGCTTTCGCATTACTGCCATCTTCAACGGCTAACGGCAGGTTTTCGGCAATCATGGTCACGGCTTTCAGTGTACAAGCGTCAGTGATCGGCGTggc                                                                                                                 >  1:87914/1‑140 (MQ=255)                   aaCGCCATACCGGCGAGGAACTGGGCATAAGCCATTGCTTCACGCGCTTTCGCATTACTGCCATCTTCAACGGCTAACGGCAGGTTTTCGGCAATCATGGTCACGGCTTTCAGTGTACAAGCGTCAGTGATCGGCGTggc                                                                                                                 <  2:514360/140‑1 (MQ=255)                   aaCGCCATACCGGCGAGGAACTGGGCATAAGCCATTGCTTCACGCGCTTTCGCATTACTGCCATCTTCAACGGCTAACGGCAGGTTTTCGGCAATCATGGTCACGGCTTTCAGTGTACAAGCGTCAGTGATCGGCGTggc                                                                                                                 <  1:85215/140‑1 (MQ=255)                    aCGCCATACCGGCGAGGAACTGGGCATAAGCCATTGCTTCACGCGCTTTCGCATTACTGCCATCTTCAACGGCTAACGGCAGGTTTTCGGCAATCATGGTCACGGCTTTCAGTGTACAAGCGTCAGTGATCGGCGTggcg                                                                                                                <  1:898290/140‑1 (MQ=255)                     cGCCATACCGGCGAGGAACTGGGCATAAGCCATTGCTTCACGCGCTTTCGCATTACTGCCATCTTCAACGGCTAACGGCAGGTTTTCGGCAATCATGGTCACGGCTTTCAGTGTACAAGCGTCAGTGATCGGCGTggcgg                                                                                                               <  2:738386/140‑1 (MQ=255)                     cGCCATACCGGCGAGGAACTGGGCATAAGCCATTGCTTCACGCGCTTTCGCATTACTGCCATCTTCAACGGCTAACGGCAGGTTTTCGGCAATCATGGTCACGGCTTTCAGTGTACAAGCGTCAGTGATCGGCGTggcgg                                                                                                               >  2:370884/1‑140 (MQ=255)                      gCCATACCGGCGAGGAACTGGGCATAAGCCATTGCTTCACGCGCTTTCGCATTACTGCCATCTTCAACGGCTAACGGCAGGTTTTCGGCAATCATGGTCACGGCTTTCAGTGTACAAGCGTCAGTGATCGGCGTggcggc                                                                                                              <  2:871442/140‑1 (MQ=255)                        cATACCGGCGAGGAACTGGGCATAAGCCATTGCTTCACGCGCTTTCGCATTACTGCCATCTTCAACGGCTAACGGCAGGTTTTCGGCAATCATGGTCACGGCTTTCAGTGTACAAGCGTCAGTGATCGGCGTGGCGGCaa                                                                                                            >  1:757216/1‑140 (MQ=255)                        cATACCGGCGAGGAACTGGGCATAAGCCATTGCTTCACGCGCTTTCGCATTACTGCCATCTTCAACGGCTAACGGCAGGTTTTCGGCAATCATGGTCACGGCTTTCAGTGTACAAGCGTCAGTGATCGGCGTGGCGGCaa                                                                                                            >  1:56825/1‑140 (MQ=255)                        cATACCGGCGAGGAACTGGGCATAAGCCATTGCTTCACGCGCTTTCGCATTACTGCCATCTTCAACGGCTAACGGCAGGTTTTCGGCAATCATGGTCACGGCTTTCAGTGTACAAGCGTCAGTGATCGGCGTGGCGGCaa                                                                                                            <  2:744434/140‑1 (MQ=255)                          tACCGGCGAGGAACTGGGCATAAGCCATTGCTTCACGCGCTTTCGCATTACTGCCATCTTCAACGGCTAACGGCAGGTTTTCGGCAATCATGGTCACGGCTTTCAGTGTACAAGCGTCAGTGATCGGCGTGGCGGCAATa                                                                                                          <  1:895962/140‑1 (MQ=255)                          tACCGGCGAGGAACTGGGCATAAGCCATTGCTTCACGCGCTTTCGCATTACTGCCATCTTCAACGGCTAACGGCAGGTTTTCGGCAATCATGGTCACGGCTTTCAGTGTACAAGCGTCAGTGATCGGCGTGGCGGCAATa                                                                                                          >  1:40/1‑140 (MQ=255)                          tACCGGCGAGGAACTGGGCATAAGCCATTGCTTCACGCGCTTTCGCATTACTGCCATCTTCAACGGCTAACGGCAGGTTTTCGGCAATCATGGTCACGGCTTTCAGTGTACAAGCGTCAGTGATCGGCGTGGCGGCAATa                                                                                                          <  2:123040/140‑1 (MQ=255)                           aCCGGCGAGGAACTGGGCATAAGCCATTGCTTCACGCGCTTTCGCATTACTGCCATCTTCAACGGCTAACGGCAGGTTTTCGGCAATCATGGTCACGGCTTTCAGTGTACAAGCGTCAGTGATCGGCGTGGCGGCAATAg                                                                                                         <  1:199602/140‑1 (MQ=255)                            ccGGCGAGGAACTGGGCATAAGCCATTGCTTCACGCGCTTTCGCATTACTGCCATCTTCAACGGCTAACGGCAGGTTTTCGGCAATCATGGTCACGGCTTTCAGTGTACAAGCGTCAGTGATCGGCGTGGCGGCAATAGa                                                                                                        <  1:884537/140‑1 (MQ=255)                               gCGAGGAACTGGGCATAAGCCATTGCTTCACGCGCTTTCGCATTACTGCCATCTTCAACGGCTAACGGCAGGTTTTCGGCAATCATGGTCACGGCTTTCAGTGTACAAGCGTCAGTGATCGGCGTGGCGGCAATAGAAAc                                                                                                     >  1:530499/1‑140 (MQ=255)                                cGAGGAACTGGGCATAAGCCATTGCTTCACGCGCTTTCGCATTACTGCCATCTTCAACGGCTAACGGCAGGTTTTCGGCAATCATGGTCACGGCTTTCAGTGTACAAGCGTCAGTGATCGGCGTGGCGGCAATAGAAACa                                                                                                    <  1:945412/140‑1 (MQ=255)                                cGAGGAACTGGGCATAAGCCATTGCTTCACGCGCTTTCGCATTACTGCCATCTTCAACGGCTAACGGCAGGTTTTCGGCAATCATGGTCACGGCTTTCAGTGTACAAGCGTCAGTGATCGGCGTGGCGGCAATAGAAACa                                                                                                    <  2:56825/140‑1 (MQ=255)                                cGAGGAACTGGGCATAAGCCATTGCTTCACGCGCTTTCGCATTACTGCCATCTTCAACGGCTAACGGCAGGTTTTCGGCAATCATGGTCACGGCTTTCAGTGTACAAGCGTCAGTGATCGGCGTGGCGGCAATAGAAACa                                                                                                    <  2:832959/140‑1 (MQ=255)                                cGAGGAACTGGGCATAAGCCATTGCTTCACGCGCTTTCGCATTACTGCCATCTTCAACGGCTAACGGCAGGTTTTCGGCAATCATGGTCACGGCTTTCAGTGTACAAGCGTCAGTGATCGGCGTGGCGGCAATAGAAACa                                                                                                    <  2:87914/140‑1 (MQ=255)                                cGAGGAACTGGGCATAAGCCATTGCTTCACGCGCTTTCGCATTACTGCCATCTTCAACGGCTAACGGCAGGTTTTCGGCAATCATGGTCACGGCTTTCAGTGTACAAGCGTCAGTGATCGGCGTGGCGGCAATAGAAACa                                                                                                    <  1:871082/140‑1 (MQ=255)                                  aGGAACTGGGCATAAGCCATTGCTTCACGCGCTTTCGCATTACTGCCATCTTCAACGGCTAACGGCAGGTTTTCGGCAATCATGGTCACGGCTTTCAGTGTACAAGCGTCAGTGATCGGCGTGGCGGCAATAGAAACata                                                                                                  >  1:332357/1‑140 (MQ=255)                                  aGGAACTGGGCATAAGCCATTGCTTCACGCGCTTTCGCATTACTGCCATCTTCAACGGCTAACGGCAGGTTTTCGGCAATCATGGTCACGGCTTTCAGGGTACAAGCGTCAGGGATCGGCGGGGCGGCAATAGAAAAata                                                                                                  >  2:100367/1‑140 (MQ=255)                                  aGGAACTGGGCATAAGCCATTGCTTCACGCGCTTTCGCATTACTGCCATCTTCAACGGCTAACGGCAGGTTTTCGGCAATCATGGATACGGCTTTCAGTGCACAAACGTACGTGATCGGCCTGGCGGCAAAAGAAACata                                                                                                  >  2:871082/1‑140 (MQ=255)                                         gggCATAAGCCATTGCTTCACGCGCTTTCGCATTACTGCCATCTTCAACGGCTAACGGCAGGTTTTCGGCAATCATGGTCACGGCTTTCAGTGTACAAGCGTCAGTGATCGGCGTGGCGGCAATAGAAACATATGCTTCg                                                                                           <  1:421163/140‑1 (MQ=255)                                          ggCATAAGCCATTGCTTCACGCGCTTTCGCATTACTGCCATCTTCAACGGCTAACGGCAGGTTTTCGGCAATCATGGTCACGGCTTTCAGTGTACAAGCGTCAGTGATCGGCGTGGCGGCAATAGAAACa                                                                                                    >  1:647697/1‑130 (MQ=255)                                          ggCATAAGCCATTGCTTCACGCGCTTTCGCATTACTGCCATCTTCAACGGCTAACGGCAGGTTTTCGGCAATCATGGTCACGGCTTTCAGTGTACAAGCGTCAGTGATCGGCGTGGCGGCAATAGAAACa                                                                                                    <  2:647697/130‑1 (MQ=255)                                              tAAGCCATTGCTTCACGCGCTTTCGCATTACTGCCATCTTCAACGGCTAACGGCAGGTTTTCGGCAATCATGGTCACGGCTTTCAGTGTACAAGCGTCAGTGATCGGCGTGGCGGCAATAGAAACATATGCTTCGATAgc                                                                                      <  2:332357/140‑1 (MQ=255)                                              tAAGCCATTGCTTCACGCGCTTTCGCATTACTGCCATCTTCAACGGCTAACGGCAGGTTTTCGGCAATCATGGTCACGGCTTTCAGTGTACAAGCGTCAGTGATCGGCGTGGCGGCAATAGAAACATATGCTTCGATAgc                                                                                      <  2:333191/140‑1 (MQ=255)                                              tAAGCCATTGCTTCACGCGCTTTCGCATTACTGCCATCTTCAACGGCTAACGGCAGGTTTTCGGCAATCATGGTCACGGCTTTCAGTGTACAAGCGTCAGTGATCGGCGTGGCGGCAATAGAAACATATGCTTCGATAgc                                                                                      <  1:958213/140‑1 (MQ=255)                                               aaGCCATTGCTTCACGCGCTTTCGCATTACTGCCATCTTCAACGGCTAACGGCAGGTTTTCGGCAATCATGGTCACGGCTTTCAGTGTACAAGCGTCAGTGATCGGCGTGGCGGCAATAGAAACATATGCTTCGATAgc                                                                                      <  2:926243/139‑1 (MQ=255)                                               aaGCCATTGCTTCACGCGCTTTCGCATTACTGCCATCTTCAACGGCTAACGGCAGGTTTTCGGCAATCATGGTCACGGCTTTCAGTGTACAAGCGTCAGTGATCGGCGTGGCGGCAATAGAAACATATGCTTCGATAgc                                                                                      >  1:926243/1‑139 (MQ=255)                                                      tGCTTCACGCGCTTTCGCATTACTGCCATCTTCAACGGCTAACGGCAGGTTTTCGGCAATCATGGTCACGGCTTTCAGTGTACAAGCGTCAGTGATCGGCGTGGCGGCAATAGAAACATATGCTTCGATAGCGTGCGTTa                                                                              >  1:864942/1‑140 (MQ=255)                                                        cTTCACGCGCTTTCGCATTACTGCCATCTTCAACGGCTAACGGCAGGTTTTCGGCAATCATGGTCACGGCTTTCAGTGTACAAGCGTCAGTGATCGGCGTGGCGGCAATAGAAACATATGCTTCGATAGCGTGCGTTAAg                                                                            <  2:361897/140‑1 (MQ=255)                                                         ttCACGCGCTTTCGCATTACTGCCATCTTCAACGGCTAACGGCAGGTTTTCGGCAATCATGGTCACGGCTTTCAGTGTACAAGCGTCAGTGATCGGCGTGGCGGCAATAGAAACATATGCTTCGATAGCGTGCGTTAAgg                                                                           <  1:370884/140‑1 (MQ=255)                                                         ttCACGCGCTTTCGCATTACTGCCATCTTCAACGGCTAACGGCAGGTTTTCGGCAATCATGGTCACGGCTTTCAGTGTACAAGCGTCAGTGATCGGCGTGGCGGCAATAGAAACATATGCTTCGATAGCGTGCGTTAAgg                                                                           <  2:393949/140‑1 (MQ=255)                                                         ttCACGCGCTTTCGCATTACTGCCATCTTCAACGGCTAACGGCAGGTTTTCGGCAATCATGGTCACGGCTTTCAGTGTACAAGCGTCAGTGATCGGCGTGGCGGCAATAGAAACATATGCTTCGATAGCGTGCGTTAAgg                                                                           <  2:235987/140‑1 (MQ=255)                                                         ttCACGCGCTTTCGCATTACTGCCATCTTCAACGGCTAACGGCAGGTTTTCGGCAATCATGGTCACGGCTTTCAGTGTACAAGCGTCAGTGATCGGCGTGGCGGCAATAGAAACATATGCTTCGATAGCGTGCGTTAAgg                                                                           <  2:757216/140‑1 (MQ=255)                                                           cACGCGCTTTCGCATTACTGCCATCTTCAACGGCTAACGGCAGGTTTTCGGCAATCATGGTCACGGCTTTCAGTGTACAAGCGTCAGTGATCGGCGTGGCGGCAATAGAAACATATGCTTCGATAGCGTGCGTTAAGGCa                                                                         >  2:71415/1‑140 (MQ=255)                                                            aCGCGCTTTCGCATTACTGCCATCTTCAACGGCTAACGGCAGGTTTTCGGCAATCATGGTCACGGCTTTCAGTGTACAAGCGTCAGTGATCGGCGTGGCGGCAATAGAAACATATGCTTCGATAGCGTGCGTTAAg                                                                            >  2:515781/1‑136 (MQ=255)                                                            aCGCGCTTTCGCATTACTGCCATCTTCAACGGCTAACGGCAGGTTTTCGGCAATCATGGTCACGGCTTTCAGTGTACAAGCGTCAGTGATCGGCGTGGCGGCAATAGAAACATATGCTTCGATAGCGTGCGTTAAg                                                                            <  1:515781/136‑1 (MQ=255)                                                            aCGCGCTTTCGCATTACTGCCATCTTCAACGGCTAACGGCAGGTTTTCGGCAATCATGGTCACGGCTTTCAGTGTACAAGCGTCAGTGATCGGCGTGGCGGCAATAGAAACATATGCTTCGATAGCGTGCGTTAAGGCAt                                                                        >  1:610944/1‑140 (MQ=255)                                                            aCGCGCTTTCGCATTACTGCCATCTTCAACGGCTAACGGCAGGTTTTCGGCAATCATGGTCACGGCTTTCAGTGTACAAGCGTCAGTGATCGGCGTGGCGGCAATAGAAACATATGCTTCGATAGCGTGCGTTAAGGCAt                                                                        >  2:875166/1‑140 (MQ=255)                                                                  tttCGCATTACTGCCATCTTCAACGGCTAACGGCAGGTTTTCGGCAATCATGGTCACGGCTTTCAGTGTACAAGCGTCAGTGATCGGCGTGGCGGCAATAGAAACATATGCTTCGATAGCGTGCGtt                                                                               <  1:648554/127‑1 (MQ=255)                                                                  tttCGCATTACTGCCATCTTCAACGGCTAACGGCAGGTTTTCGGCAATCATGGTCACGGCTTTCAGTGTACAAGCGTCAGTGATCGGCGTGGCGGCAATAGAAACATATGCTTCGATAGCGTGCGtt                                                                               >  2:648554/1‑127 (MQ=255)                                                                            cTGCCATCCTCAACGGCTAACGGCAGGTTTTCGGCAATCATGGTCACGGCTTTCAGTGTACAAGCGTCAGTGATCGGCGTGGCGGCAATAGAAACATATGCTTCGATAGCGTGCGTTAAGGCATCCATACCCGTTgcggc                                                        >  2:423572/1‑140 (MQ=255)                                                                                   cTTCAACGGCTAACGGCAGGTTTTCGGCAATCATGGTCACGGCTTTCAGTGTACAAGCGTCAGTGATCGGCGTGGCGGCAATAGAAACATATGCTTCGATAGCGTGCGTTAAGGCATCCATACCCGTTGCGGCGGTCAGt                                                 <  2:864942/140‑1 (MQ=255)                                                                                    ttCAACGGCTAACGGCAGGTTTTCGGCAATCATGGTCACGGCTTTCAGTGTACAAGCGTCAGTGATCGGCGTGGCGGCAATAGAAACATATGCTTCGATAGCGTGCGTTAAGGCATCCATACCCGTTGCGGCGGTCAGTg                                                >  2:896543/1‑140 (MQ=255)                                                                                    ttCAACGGCTAACGGCAGGTTTTCGGCAATCATGGTCACGGCTTTCAGTGTACAAGCGTCAGTGATCGGCGTGGCGGCAATAGAAACATATGCTTCGATAGCGTGCGTTAAGGCATCCATACCCGTTGCGGCGGTCAGTg                                                >  1:250153/1‑140 (MQ=255)                                                                                    ttCAACGGCTAACGGCAGGTTTTCGGCAATCATGGTCACGGCTTTCAGTGTACAAGCGTCAGTGATCGGCGTGGCGGCAATAGAAACATATGCTTCGATAGCGTGCGTTAAGGCATCCATACCCGTTGCGGCGGTCAGTg                                                >  1:699424/1‑140 (MQ=255)                                                                                    ttCAACGGCTAACGGCAGGTTTTCGGCAATCATGGTCACGGCTTTCAGTGTACAAGCGTCAGTGATCGGCGTGGCGGCAATAGAAACATATGCTTCGATAGCGTGCGTTAAGGCATCCATACCCGTTGCGGCGGTCAGTg                                                >  1:876798/1‑140 (MQ=255)                                                                                    ttCAACGGCTAACGGCAGGTTTTCGGCAATCATGGTCACGGCTTTCAGTGTACAAGCGTCAGTGATCGGCGTGGCGGCAATAGAAACATATGCTTCGATAGCGGGCGTTAAGGCATCCATACCCGTTGcggcgggccggg                                                >  2:294061/1‑134 (MQ=255)                                                                                     tCAACGGCTAACGGCAGGTTTTCGGCAATCATGGTCACGGCTTTCAGTGTACAAGCGTCAGTGATCGGCGTGGCGGCAATAGAAACATATGCTTCGATAGCGTGCGTTAAGGCATCCATACCCGTTGCGGCGGTCAGTGa                                               <  1:875166/140‑1 (MQ=255)                                                                                     tCAACGGCTAACGGCAGGTTTTCGGCAATCATGGTCACGGCTTTCAGTGTACAAGCGTCAGTGATCGGCGTGGCGGCAATAGAAACATATGCTTCGATAGCGTGCGTTAAGGCATCCATACCCGTTGCGGCGGTCAGTGa                                               <  2:594181/140‑1 (MQ=255)                                                                                      cAACGGCTAACGGCAGGTTTTCGGCAATCATGGTCACGGCTTTCAGTGTACAAGCGTCAGTGATCGGCGTGGCGGCAATAGAAACATATGCTTCGATAGCGTGCGTTAAGGCATCCATACCCGTTGCGGCGGTCAGTGAc                                              <  1:530202/140‑1 (MQ=255)                                                                                       aaCGGCTAACGGCAGGTTTTCGGCAATCATGGTCACGGCTTTCAGTGTACAAGCGTCAGTGATCGGCGTGGCGGCAATAGAAACATATGCTTCGATAGCGTGCGTTAAGGCATCCATACCCGTTGCGGCGGGCAggggat                                             >  2:530202/1‑135 (MQ=255)                                                                                        aCGGCTAACGGCAGGTTTTCGGCAATCATGGTCACGGCTTTCAGTGTACAAGCGTCAGTGATCGGCGTGGCGGCAATAGAAACATATGCTTCGATAGCGTGCGTTAAGGCATCCATACCCGTTGCGGCGGTCAGTGACtt                                            <  2:40/140‑1 (MQ=255)                                                                                          ggCTAACGGCAGGTTTTCGGCAATCATGGTCACGGCTTTCAGTGTACAAGCGTCAGTGATCGGCGTGGCGGCAATAGAAACATATGCTTCGATAGCGTGCGTTAAGGCATCCATACCCGTTGCGGCGGTCAGTGACTTCg                                          <  1:71415/140‑1 (MQ=255)                                                                                          ggCTAACGGCAGGTTTTCGGCAATCATGGTCACGGCTTTCAGTGTACAAGCGTCAGTGATCGGCGTGGCGGCAATAGAAACATATGCTTCGATAGCGTGCGTTAAGGCATCCATACCCGTTGCGGCGGTCAGTGACTTCg                                          <  1:896543/140‑1 (MQ=255)                                                                                            cTAACGGCAGGTTTTCGGCAATCATGGTCACGGCTTTCAGTGTACAAGCGTCAGTGATCCGCGTGGCGGCAATAGAAACATATGCTTCCATAGCGGGCGTTAAAGCATCCCTACCCGTTGCGGCGGGCAGTGACTTCggg                                        >  2:590996/1‑139 (MQ=255)                                                                                             tAACGGCAGGTTTTCGGCAATCATGGTCACGGCTTTCAGTGTACAAGCGTCAGTGATCGGCGTGGCGGCAATAGAAACATATGCTTCGATAGCGTGCGTTAAGGCATCCATACCCGTTGCGGCGGTCAGTGACTTCGGCa                                       <  1:832887/140‑1 (MQ=255)                                                                                             tAACGGCAGGTTTTCGGCAATCATGGTCACGGCTTTCAGTGTACAAGCGTCAGTGATCGGCGTGGCGGCAATAGAAACATATGCTTCGATAGCGTGCGTTAAGGCATCCATACCCGTTGCGGCGGTCAGTGACTTCGGCa                                       <  1:100367/140‑1 (MQ=255)                                                                                              aaCGGCAGGTTTTCGGCAATCATGGTCACGGCTTTCAGTGTACAAGCGTCAGTGATCGGCGTGGCGGCAATAGAAACATATGCTTCGATAGCGTGCGTTAAGGCATCCATACCCGTTGCGGCGGGCAGGGGATTTGGCAt                                      >  2:535392/1‑140 (MQ=255)                                                                                                  gCAGGTTTTCGGCAATCATGGTCACGGCTTTCAGTGTACAAGCGTCAGTGATCGGCGTGGCGGCAATAGAAACATATGCTTCGATAGCGTGCGTTAAGGCATCCATACCCGTTGCGGCGGTCAGTGACTTCGGCATACCa                                  <  2:876798/140‑1 (MQ=255)                                                                                                  gCAGGTTTTCGGCAATCATGGTCACGGCTTTCAGTGTACAAGCGTCAGTGATCGGCGTGGCGGCAATAGAAACATATGCTTCGATAGCGTGCGTTAAGGCATCCATACCCGTTGCGGCGGTCAGTGACTTCGGCATACCa                                  <  1:294061/140‑1 (MQ=255)                                                                                                  gCAGGTTTTCGGCAATCATGGTCACGGCTTTCAGTGTACAAGCGTCAGTGATCGGCGTGGCGGCAATAGAAACATATGCTTCGATAGCGTGCGTTAAGGCATCCATACCCGTTGCGGCGGTCAGTGACTTCGGCATACCa                                  <  2:576694/140‑1 (MQ=255)                                                                                                    aGGTTTTCGGCAATCATGGTCACGGCTTTCAGTGTACAAGCGTCAGTGATCGGCGTGGCGGCAATAGAAACATATGCTTCGATAGCGTGCGTTAAGGCATCCATACCCGTTGCGGCGGTCAGTGAc                                              >  1:794273/1‑126 (MQ=255)                                                                                                    aGGTTTTCGACAATCATGGTCACGGCTTTCAGTGTACAAGCGTCAGTGATCGGCGTGGCGGCAATAGAAACATATGCTTCGATAGCGTGCGTTAAGGCATCCATACCCGTTGCGGCGGTCAGTGAc                                              <  2:794273/126‑1 (MQ=255)                                                                                                                aTCATGGTCACGGCTTTCAGTGTACAAGCGTCAGTGATCGGCGTGGCGGCAATAGAAACATATGCTTCGATAGCGTGCGTTAAGGCATCCATACCCGTTGCGGCGGTCAGTGACTTCGGCATACCAATCATCAGAgagga                    <  1:328589/140‑1 (MQ=255)                                                                                                                 tCATGGTCACGGCTTTCAGTGTACAAGCGTCAGTGATCGGCGTGGCGGCAATAGAAACATATGCTTCGATAGCGTGCGTTAAGGCATCCATACCCGTTGCGGCGGTCAGTGACTTCGGCATACCAATCATCAGAgaggag                   >  1:274878/1‑140 (MQ=255)                                                                                                                               ttCAGTGTACAAGCGTCAGTGATCGGCGTGGCGGCAATAGAAACATATGCTTCGATAGCGTGCGTTAAGGCATCCATACCCGTTGCGGCGGTCAGTGACTTCGGCATACCAATCATCAGAGAGGAGTCATTGACAGAAag     <  2:553544/140‑1 (MQ=255)                                                                                                                                tCAGTGTACAAGCGTCAGTGATCGGCGTGGCGGCAATAGAAACATATGCTTCGATAGCGTGCGTTAAGGCATCCATACCCGTTGCGGCGGTCAGTGACTTCGGCATACCAATCATCAGAGAGGAGTCATTGACAGAAagc    >  1:242906/1‑140 (MQ=255)                                                                                                                                 cAGTGTACAAGCGTCAGTGATCGGCGTGGCGGCAATAGAAACATATGCTTCGATAGCGTGCGTTAAGGCATCCATACCCGTTGCGGCGGTCAGTGACTTCGGCATACCAATCATCAGAGAGGAGTCATTGACAGAAagca   <  1:535392/140‑1 (MQ=255)                                                                                                                                  aGTGTACAAGCGTCAGTGATCGGCGTGGCGGCAATAGAAACATATGCTTCGATAGCGTGCGTTAAGGCATCCATTCCCGTTGCGGCGGTCAGTGACTTCGGCATACCAATCATCAGAGAGGAGTCATTGACAGAAagcag  <  1:886554/140‑1 (MQ=255)                                                                                                                                  aGTGTACAAGCGTCAGTGATCGGCGTGGCGGCAATAGAAACATATGCTTCGATAGCGTGCGTTAAGGCATCCATACCCGTTGCGGCGGTCAGTGACTTCGGCATACCAATCATCAGAGAGGAGTCATTGACAGAAagcag  <  1:770760/140‑1 (MQ=255)                                                                                                                                      |                                                                                                                                        CCCAGAGAAGCATTATTGAACGCCATACCGGCGAGGAACTGGGCATAAGCCATTGCTTCACGCGCTTTCGCATTACTGCCATCTTCAACGGCTAACGGCAGGTTTTCGGCAATCATGGTCACGGCTTTCAGTGCACAAGCGTCAGTGATCGGCGTGGCGGCAATAGAAACATATGCTTCGATAGCGTGCGTTAAGGCATCCATACCCGTTGCGGCGGTCAGTGACTTCGGCATACCAATCATCAGAGAGGAGTCATTGACAGAAAGCAG  >  NC_000913/3757049‑3757317

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑