Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,804,059	A→G	F27L (TTC→CTC)	waaB ←	UDP‑D‑galactose:(glucosyl)lipopolysaccharide‑1, 6‑D‑galactosyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,804,059	0	A	G	100.0%	152.2 / NA	42	F27L (TTC→CTC)	waaB	UDP‑D‑galactose:(glucosyl)lipopolysaccharide‑1, 6‑D‑galactosyltransferase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (26/16);  total (26/16)

TGTTTCGCCCGACGCAAAAAACCTAAACGAATATTGGAAAATGAACATGAGTATTTAATACCTTCCAGCCATCCCTTATCCATTTTGTCATTACGACAAAAGAAAAACATTTCACTTTGGATATGTTGTTGCCTGAAGGTAGTAATAACATCACGGATGACAGTTTCCATGCCGCCAAATCCAGAAACAGCTTCGCCGATAAACGCTATTTTCATAGAAACTCTCATTAATTGAGTAAAACTCACTGTTTTAACGGTTTTATTTGTCTATA  >  NC_000913/3803923‑3804193                                                                                                                                         |                                                                                                                                       tGTTTCGCCCGACGCAAAAAACCTAAACGAATATTGGAAAATGAACATGAGTATTTAATACCTTCCAGCCATCCCTTATCCATTTTGTCATTACGACAAAAGAAAAACATTTCACTTTGGATATGTTGTTGCCTGAGGgt                                                                                                                                     >  1:874453/1‑140 (MQ=255)   tttCGCCCGACGCAAAAAACCTAAACGAATATTGGAAAATGAACATGAGTATTTAATACCTTCCAGCCATCCCTTATCCATTTTGTCATTACGACAAAAGAAAAACATTTCACTTTGGATATGTTGTTGCCTGAGGgtag                                                                                                                                   >  2:227978/1‑140 (MQ=255)        cccGACGCAAAAAACCTAAACGAATATTGGAAAATGAACATGAGTATTTAATACCTTCCAGCCATCCCTTATCCATTTTGTCATTACGACAAAAGAAAAACATTTCACTTTGGATATGTTGTTGCCTGAGGGTAGtaata                                                                                                                              >  2:873749/1‑140 (MQ=255)        cccGACGCAAAAAACCTAAACGAATATTGGAAAATGAACATGAGTATTTAATACCTTCCAGCCATCCCTTATCCATTTTGTCATTACGACAAAAGAAAAACATTTCACTTTGGATATGTTGTTGCCTGAGGGTAGtaata                                                                                                                              >  2:284935/1‑140 (MQ=255)         ccGACGCAAAAAACCTAAACGAATATTGGAAAATGAACATGAGTATTTAATACCTTCCAGCCATCCCTTATCCATTTTGTCATTACGACAAAAGAAAAACATTTCACTTTGGATATGTTGTTGCCTGAGGGTAGtaataa                                                                                                                             >  1:326688/1‑140 (MQ=255)            aCGCAAAAAACCTAAACGAATATTGGAAAATGAACATGAGTATTTAATACCTTCCAGCCATCCCTTATCCATTTTGTCATTACGACAAAAGAAAAACATTTCACTTTGGATATGTTGTTGCCTGAGGGTAGTAATAACaa                                                                                                                          >  1:152479/1‑139 (MQ=255)             cGCAAAAAACCTAAACGAATATTGGAAAATGAACATGAGTATTTAATACCTTCCAGCCATCCCTTATCCATTTTGTCATTACGACAAAAGAAAAACATTTCACTTTGGATATGTTGTTGCCTGAGGGTAGTAGTAACATc                                                                                                                         <  2:258196/140‑1 (MQ=255)             cGCAAAAAACCTAAACGAATATTGGAAAATGAACATGAGTATTTAATACCTTCCAGCCATCCCTTATCCATTTTGTCATTACGACAAAAGAAAAACATTTCACTTTGGATATGTTGTTGCCTGAGGGTAGTAATAACATc                                                                                                                         <  2:41914/140‑1 (MQ=255)             cGCAAAAAACCTAAACGAATATTGGAAAATGAACATGAGTATTTAATACCTTCCAGCCATCCCTTATCCATTTTGTCATTACGACAAAAGAAAAACATTTCACTTTGGATATGTTGTTGCCTGAGGGTAGTAATAACATc                                                                                                                         <  1:873749/140‑1 (MQ=255)              gCAAAAAACCTAAACGAATATTGGAAAATGAACATGAGTATTTAATACCTTCCAGCCATCCCTTATCCATTTTGTCATTACGACAAAAGAAAAACATTTCACTTTGGATATGTTGTTGCCTGAGGGTAGTAATAACATCa                                                                                                                        <  1:77959/140‑1 (MQ=255)                  aaaaCCTAAACGAATATTGGAAAATGAACATGAGTATTTAATACCTTCCAGCCATCCCTTATCCATTTTGTCATTACGACAAAAGAAAAACATTTCACTTTGGATATGTTGTTGCCTGAGGGTAGTAATAACATCACGGa                                                                                                                    <  2:315063/140‑1 (MQ=255)                                  ttGGAAAATGAACATGAGTATTTAATACCTTCCAGCCATCCCTTATCCATTTTGTCATTACTACAAAAGAAAAACATTTCACTTTGGATATGTTGTTGCCTGAGGGTAGTAATAACATCACGGATGACAGTTTCCATgcc                                                                                                    <  2:273901/140‑1 (MQ=255)                                                 gAGTATTTATTACCTTCCAGCCATCCCTTATCCATTTTGTCATTACGACAAAAGAAAAACATTTCACTTTGGATATGTTGTTGCCTGAGGGTAGTAATAACATCACGGATGACAGTTTCCATGCCGCCAAATCCAGAAAc                                                                                     >  2:951202/1‑140 (MQ=255)                                                           tACCTTCCAGCCATCCCTTATCCATTTTGTCATTACGACAAAAGAAAAACATTTCACTTTGGATATGTTGTTGCCTGAGGGTAGTAATAACATCACGGATGACAGTTTCCATGCCGCCAAATCCAGAAACAGCTTCGCCg                                                                           >  1:821/1‑140 (MQ=255)                                                                tCCAGCCATCCCTTATCCATTTTGTCATTACGACAAAAGAAAAACATTTCACTTTGGATATGTTGTTGCCTGAGGGTAGTAATAACATCACGGATGACAGTTTCCATGCCGCCAAATCCAGAAACAGCTTCGCCGATaaa                                                                      >  2:721448/1‑140 (MQ=255)                                                                tCCAGCCATCCCTTATCCATTTTGTCATTACGACAAAAGAAAAACATTTCACTTTGGATATGTTGTTGCCTGAGGGTAGTAATAACATCACGGATGACAGTTTCCATGCCGCCAAATCCAGAAACAGCTTCGCCGATaaa                                                                      >  2:716750/1‑140 (MQ=255)                                                                tCCAGCCATCCCTTATCCATTTTGTCATTACGACAAAAGAAAAACATTTCACTTTGGATATGTTGTTGCCTGAGGGTAGTAATAACATCACGGATGACAGTTTCCATGCCGCCAAATCCAGAAACAGCTTCGCCGATaaa                                                                      >  2:530777/1‑140 (MQ=255)                                                                tCCAGCCATCCCTTATCCATTTTGTCATTACGACAAAAGAAAAACATTTCACTTTGGATATGTTGTTGCCTGAGGGTAGTAATAACATCACGGATGACAGTTTCCATGCCGCCAAATCCAGAAACAGCTTCGCCGATaaa                                                                      >  2:430423/1‑140 (MQ=255)                                                                               tCCATTTTGTCATTACGACAAAAGAAAAACATTTCACTTTGGATATGTTGTTGCCTGAGGGTAGTAATAACATCACGGATGACAGTTTCCATGCCGCCAAATCCAGAAACAGCTTCGCCGATAAACGCTATTTTCATAGa                                                       >  1:678051/1‑140 (MQ=255)                                                                                 cATTTTGTCATTACGACAAAAGAAAAACATTTCACTTTGGATATGTTGTTGCCTGAGGGTAGTAATAACATCACGGATGACAGTTTCCATGCCGCCAAATCCAGAAACAGCTTCGCCGATAAACGCTATTTTCATAGaaa                                                     <  2:821/140‑1 (MQ=255)                                                                                 cATTTTGTCATTACGACAAAAGAAAAACATTTCACTTTGGATATGTTGTTGCCTGAGGGTAGTAATAACATCACGGATGACAGTTTCCATGCCGCCAAATCCAGAAACAGCTTCGCCGATAAACGCTATTTTCATAGaaa                                                     <  1:247075/140‑1 (MQ=255)                                                                                 cATTTTGTCATTACGACAAAAGAAAAACATTTCACTTTGGATATGTTGTTGCCTGAGGGTAGTAATAACATCACGGATGACAGTTTCCATGCCGCCAAATCCAGAAACAGCTTCGCCGATAAACGCTATTTTCATAGaaa                                                     <  1:227978/140‑1 (MQ=255)                                                                                   ttttGTCATTACGACAAAAGAAAAACATTTCACTTTGGATATGTTGTTGCCTGAGGGTAGTAATAACATCACGGATGACAGTTTCCATGCCGCCAAATCCAGAAACAGCTTCGCCGATAAACGCTATTTTCATAGAAAct                                                   >  1:558764/1‑140 (MQ=255)                                                                                   ttttGTCATTACGACAAAAGAAAAACATTTCACTTTGGATATGTTGTTGCCTGAGGGTAGTAATAACATCACGGATGACAGTCTCCATGCCGCCAAATCCAGAAACAGCTTCGCCGATAAACGCTATTTTCATAGAAAct                                                   <  1:284935/140‑1 (MQ=255)                                                                                    tttGTCATTACGACAAAAGAAAAACATTTCACTTTGGATATGTTGTTGCCTGAGGGTAGTAATAACATCACGGATGACAGTTTCCATGCCGCCAAATCCAGAAATAGCTTCGCCGATAAACGCTATTTTCATAGAAActc                                                  >  2:670741/1‑140 (MQ=255)                                                                                    tttGTCATTACGACAAAAGAAAAACATTTCACTTTGGATATGTTGTTGCCTGAGGGTAGTAATAACATCACGGATGACAGTTTCCATGCCGCCAAATCCAGAAACAGCTTCGCCGATAAACGCTATTTTCATAGAAActc                                                  >  1:940578/1‑140 (MQ=255)                                                                                     ttGTCATTACGACAAAAGAAAAACATTTCACTTTGGATATGTTGTTGCCTGAGGGTAGTAATAACATCACGGATGACAGTTTCCATGCCGCCAAATCCAGAAACAGCTTCGCCGATAAACGCTATTTTCATAGAAActct                                                 >  1:587382/1‑140 (MQ=255)                                                                                        tCATTACGACAAAAGAAAAACATTTCACTTTGGATATGTTGTTGCCTGAGGGTAGTAATAACATCACGGATGACAGTTTCCATGCCGCCAAATCCAGAAACAGCTTCGCCGATAAACGCTATTTTCATAGAAACTCTCAt                                              >  1:102556/1‑140 (MQ=255)                                                                                           ttACGACAAAAGAAAAACATTTCACTTTGGATATGTTGTTGCCTGAGGGTAGTAATAACATCACGGATGACAGTTTCCATGCCGCCAAATCCAGAAACAGCTTCGCCGATAAACGCTATTTTCATAGAAACTCTCATTaa                                           <  2:558764/140‑1 (MQ=255)                                                                                            tACGACAAAAGAAAAACATTTCACTTTGGATATGTTGTTGCCTGAGGGTAGTAATAACATCACGGATGACAGTTTCCATGCCGCCAAATCCAGAAACAGCTTCGCCGATAAACGCTATTTTCATAGAAACTCTCATTAAt                                          >  2:827463/1‑140 (MQ=255)                                                                                             aCGACAAAAGAAAAACATTTCACTTTGGATATGTTGTTGCCTGAGGGTAGTAATAACATCACGGATGACAGTTTCCATGCCGCCAAATCCAGAAACAGCTTCGCCGATAAACGCTATTTTCATAGAAACTCTCATTaa                                           <  1:857797/138‑1 (MQ=255)                                                                                             aCGACAAAAGAAAAACATTTCACTTTGGATATGTTGTTGCCTGAGGGTAGTAATAACATCACGGATGACAGTTTCCATGCCGCCAAATCCAGAAACAGCTTCGCCGATAAACGCTATTTTCATAGAAACTCTCATTaa                                           >  2:857797/1‑138 (MQ=255)                                                                                                          aaCATTTCACTTTGGATATGTTGTTGCCTGAGGGTAGTAATAACATCACGGATGACAGTTTCCATGCCGCCAAATCCAGAAACAGCTTCGCCGATAAACGCTATTTTCATAGAAACTCTCATTAATTGAGTAAAACTCAc                            <  1:827463/140‑1 (MQ=255)                                                                                                               ttCACTTTGGATATGTTGTTGCCTGAGGGTAGTAATAACATCACGGATGACAGTTTCCATGCCGCCAAATCCAGAAACAGCTTCGCCGATAAACGCTATTTTCATAGAAACTCTCATTAATTGAGTAAAACTCACTGttt                       >  2:11133/1‑140 (MQ=255)                                                                                                               ttCACTTTGGATATGTTGTTGCCTGAGGGTAGTAATAACATCACGGATGACAGTTTCCATGCCGCCAAATCCAGAAACAGCTTCGCCGATAAACGCTATTTTCATAGAAACTCTCATTAATTGAGTAAAACTCACTGttt                       >  2:809166/1‑140 (MQ=255)                                                                                                                 cACTTTGGATATGTTGTTGCCTGAGGGTAGTAATAACATCACGGATGACAGTTTCCATGCCGCCAAATCCAGAAACAGCTTCGCCGATAAACGCTATTTTCATAGAAACTCTCATTAATTGAGTAAAACTCACTGTTTTa                     <  1:11133/140‑1 (MQ=255)                                                                                                                             gttgttGCCTGAGGGTAGTAATAACATCACGGATGACAGTTTCCATGCCGCCAAATCCAGAAACAGCTTCGCCGATAAACGCTATTTTCATAGAAACTCTCATTAATTGAGTAAAACTCACTGTTTTAACGGTTTTAttt         >  2:376664/1‑140 (MQ=255)                                                                                                                                  tgCCTGAGGGTAGTAATAACATCACGGATGACAGTTTCCATGCCGCCAAATCCAGAAACAGCTTCGCCGATAAACGCTATTTTCATAGAAACTCTCATTAATTGAGTAAAACTCACTGTTTTaa                    >  1:279062/1‑124 (MQ=255)                                                                                                                                  tgCCTGAGGGTAGTAATAACATCACGGATGACAGTTTCCATGCCGCCAAATCCAGAAACAGCTTCGCCGATAAACGCTATTTTCATAGAAACTCTCATTAATTGAGTAAAACTCACTGTTTTaa                    <  2:279062/124‑1 (MQ=255)                                                                                                                                  tgCCTGAGGGTAGTAATAACATCACGGATGACAGTTTCCATGCCGCCAAATCCAGAAACAGCTTCGCCGATAAACGCTATTTTCATAGAAACTCTCATTAATTGAGTAAAACTCACTGTTTTAACGGTTTTAt           <  2:317846/133‑1 (MQ=255)                                                                                                                                  tgCCTGAGGGTAGTAATAACATCACGGATGACAGTTTCCATGCCGCCAAATCCAGAAACAGCTTCGCCGATAAACGCTATTTTCATAGAAACTCTCATTAATTGAGTAAAACTCACTGTTTTAACGGTTTTAt           >  1:317846/1‑133 (MQ=255)                                                                                                                                    ccTGAGGGTAGTAATAACATCACGGATGACAGTTTCCATGCCGCCAAATCCAGAAACAGCTTCGCCGATAAACGCTATTTTCATAGAAACTCTCATTAATTGAGTAAAACTCACTGTTTTAACGGTTTTATTTGTCtata  >  1:488180/1‑140 (MQ=255)                                                                                                                                         |                                                                                                                                       TGTTTCGCCCGACGCAAAAAACCTAAACGAATATTGGAAAATGAACATGAGTATTTAATACCTTCCAGCCATCCCTTATCCATTTTGTCATTACGACAAAAGAAAAACATTTCACTTTGGATATGTTGTTGCCTGAAGGTAGTAATAACATCACGGATGACAGTTTCCATGCCGCCAAATCCAGAAACAGCTTCGCCGATAAACGCTATTTTCATAGAAACTCTCATTAATTGAGTAAAACTCACTGTTTTAACGGTTTTATTTGTCTATA  >  NC_000913/3803923‑3804193

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑