Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,395,056	G→A	G331S (GGC→AGC)	nnr →	NAD(P)HX epimerase/NAD(P)HX dehydratase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,395,056	0	G	A	100.0%	214.6 / NA	60	G331S (GGC→AGC)	nnr	NAD(P)HX epimerase/NAD(P)HX dehydratase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (33/27);  total (33/27)

AGTACTGACCCGCAGTGAAAACATTGCGCCGCTGCTGACTGCACGACCGGAATTGATGGTGCATGAACTGACGATGGACTCTCTTACCGAAAGCCTGGAATGGGCCGATGTGGTGGTGATTGGTCCCGGTCTGGGCCAGCAAGAGTGGGGGAAAAAAGCACTGCAAAAAGTTGAGAATTTTCGCAAACCGATGTTGTGGGATGCCGATGCATTGAACCTGCTGGCAATCAATCCCGATAAGCGTCACAATCGCGTGATCACGCCGCA  >  NC_000913/4394923‑4395189                                                                                                                                      |                                                                                                                                      aGTACTGACCCGCAGTGAAAACATTGCGCCGCTGCTGACTGCACGACCGGAATTGATGGTGCATGAACTGACGATGGACTCTCTTACCGAAAGCCTGGAATGGGCCGATGTGGTGGTGATTGGTCCCGGTCTGAGCCAGc                                                                                                                                 >  1:649136/1‑140 (MQ=255) aGTACTGACCCGCAGTGAAAACATTGCGCCGCTGCTGACTGCACGACCGGAATTGATGGTGCATGAACTGACGATGGACTCTCTTACCGAAAGCCTGGAATGGGCCGATGTGGTGGTGATTGGTCCCGGTCTGAGCCAGc                                                                                                                                 >  2:232897/1‑140 (MQ=255)    aCTGACCCGCAGTGAAAACATTGCGCCGCTGCTGACTGCACGACCGGAATTGATGGTGCATGAACTGACGATGGACTCTCTTACCGAAAGCCTGGAATGGGCCGATGTGGTGGTGATTGGTCCCGGTCTGAGCCAGCAag                                                                                                                              <  2:159168/140‑1 (MQ=255)    aCTGACCCGCAGTGAAAACATTGCGCCGCTGCTGACTGCACGACCGGAATTGATGGTGCATGAACTGACGATGGACTCTCTTACCGAAAGCCTGGAATGGGCCGATGTGGTGGTGATTGGTCCCGGTCTGAGCCAGCAag                                                                                                                              <  2:487346/140‑1 (MQ=255)        aCCCGCAGTGAAAACATTGCGCCGCTGCTGACTGCACGACCGGAATTGATGGTGCATGAACTGACGATGGACTCTCTTACCGAAAGCCTGGAATGGGCCGATGTGGTGGTGATTGGTCCCGGTCTGAGCCAGCAAGAGTg                                                                                                                          >  2:355874/1‑140 (MQ=255)        aCCCGCAGTGAAAACATTGCGCCGCTGCTGACTGCACGACCGGAATTGATGGTGCATGAACTGACGATGGACTCTCTTACCGAAAGCCTGGAATGGGCCGATGTGGTGGTGATTGGTCCCGGTCTGAGCCAGCAAGAGTg                                                                                                                          >  1:425355/1‑140 (MQ=255)         cccGCAGTGAAAACATTGCGCCGCTGCTGACTGCACGACCGGAATTGATGGTGCATGAACTGACGATGGACTCTCTTACCGAAAGCCTGGAATGGGCCGATGTGGTGGTGATTGGTCCCGGTCTGAGCCAGCAAGAGTgg                                                                                                                         <  1:55866/140‑1 (MQ=255)          ccGCAGTGAAAACATTGCGCCGCTGCTGACTGCACGACCGGAATTGATGGTGCATGAACTGACGATGGACTCTCTTACCGCAAGCCTGGAATGGGCCGATGTGGTGGTGATTGGTCCCGGTCTGAGCCAGCAAGAGTggg                                                                                                                        <  2:672823/140‑1 (MQ=255)           cGCAGTGAAAACATTGCGCCGCTGCTGACTGCACGACCGGAATTGATGGTGCATGAACTGACGATGGACTCTCTTACCGAAAGCCTGGAATGGGCCGATGTGGTGGTGATTGGTCCCGGTCTGAGCCAGCAAGAGTgggg                                                                                                                       >  1:687169/1‑140 (MQ=255)             cAGTGAAAACATTGCGCCGCTGCTGACTGCACGACCGGAATTGATGGTGCATGAACTGACGATGGACTCTCTTACCGAAAGCCTGGAATGGGCCGATGTGGTGGTGATTGGTCCCGGTCTGAGCCAGCAAGAGTGGGGGa                                                                                                                     >  2:720030/1‑140 (MQ=255)                     aCATTGCGCCGCTGCTGACTGCACGACCGGAATTGATGGTGCATGAACTGACGATGGACTCTCTTACCGAAAGCCTGGAATGGGCCGATGTGGTGGTGATTGGTCCCGGTCTGAGCCAGCAAGAGTGGGGGAAAAAAGCa                                                                                                             <  2:767501/140‑1 (MQ=255)                     aCATTGCGCCGCTGCTGACTGCACGACCGGAATTGATGGTGCATGAACTGACGATGGACTCTCTTACCGAAAGCCTGGAATGGGCCGATGTGGTGGTGATTGGTCCCGGTCTGAGCCAGCAAGAGTGGGGGAAAAAAGCa                                                                                                             <  2:469599/140‑1 (MQ=255)                          gcgcCGCTGCTGACTGCACGACCGGAATTGATGGTGCATGAACTGACGATGGACTCTCTTACCGAAAGCCTGGAATGGGCCGATGTGGTGGTGATTGGTCCCGGTCTGAGCCAGCAAGAGTGGGGGAAAAAAGCACTGCa                                                                                                        >  2:52058/1‑140 (MQ=255)                          gcgcCGCTGCTGACTGCACGACCGGAATTGATGGTGCATGAACTGACGATGGACTCTCTTACCGAAAGCCTGGAATGGGCCGATGTGGTGGTGATTGGTCCCGGTCTGAGCCAGCAAGAGTGGGGGAAAAAAGCACTGCa                                                                                                        >  2:655756/1‑140 (MQ=255)                            gccgcTGCTGACTGCACGACCGGAATTGATGGTGCATGAACTGACGATGGACTCTCTTACCGAAAGCCTGGAATGGGCCGATGTGGTGGTGATTGGTCCCGGTCTGAGCCAGCAAGAGTGGGGGAAAAAAGCACTGCaaa                                                                                                      >  2:138439/1‑140 (MQ=255)                                          cACGACCGGAATTGATGGTGCATGAACTGACGATGGACTCTCTTACCGAAAGCCTGGAATGGGCCGATGTGGTGGTGATTGGTCCCGGTCTGAGCCAGCAAGAGTGGGGGAAAAAAGCACTGCAAAAAGTTGAGAAtttt                                                                                        >  2:605840/1‑140 (MQ=255)                                          cACGACCGGAATTGATGGTGCATGAACTGACGATGGACTCTCTTACCGAAAGCCTGGAATGGGCCGATGTGGTGGTGATTGGTCCCGGTCTGAGCCAGCAAGAGTGGGGGAAAAAAGCACTGCAAAAAGTTGAGAAtttt                                                                                        >  1:608600/1‑140 (MQ=255)                                             gACCGGAATTGATGGTGCATGAACTGACGATGGACTCTCTTACCGAAAGCCTGGAATGGGCCGATGTGGTGGTGATTGGTCCCGGTCTGAGCCAGCAAGAGTGGGGGAAAAAAGCACTGCAAAAAGTTGAGAATTTTCGc                                                                                     >  1:339431/1‑140 (MQ=255)                                             gACCGGAATTGATGGTGCATGAACTGACGATGGACTCTCTTACCGAAAGCCTGGAATGGGCCGATGTGGTGGTGATTGGTCCCGGTCTGAGCCAGCAAGAGTGGGGGAAAAAAGCACTGCAAAAAGTTGAGAATTTTCGc                                                                                     >  2:210273/1‑140 (MQ=255)                                             gACCGGAATTGATGGTGCATGAACTGACGATGGACTCTCTTACCGAAAGCCTGGAATGGGCCGATGTGGTGGTGATTGGTCCCGGTCTGAGCCAGCAAGAGTGAGGGAAAAAAGCACTGCAAAAAGTTGAGAATTTTCGc                                                                                     >  1:667725/1‑140 (MQ=255)                                                 ggAATTGATGGTGCATGAACTGACGATGGACTCTCTTACCGAAAGCCTGGAATGGGCCGATGTGGTGGTGATTGGTCCCGGTCTGAGCCAGCAAGAGTGGGGGAAAAAAGCACTGCAAAAAGTTGAGAATTTTCGCAAAc                                                                                 <  2:425355/140‑1 (MQ=255)                                                 ggAATTGATGGTGCATGAACTGACGATGGACTCTCTTACCGAAAGCCTGGAATGGGCCGATGTGGTGGTGATTGGTCCCGGTCTGAGCCAGCAAGAGTGGGGGAAAAAAGCACTGCAAAAAGTTGAGAATTTTCGCAAAc                                                                                 <  2:347523/140‑1 (MQ=255)                                                    aTTGATGGTGCATGAACTGACGATGGACTCTCTTACCGAAAGCCTGGAATGGGCCGATGTGGTGGTGATTGGTCCCGGTCTGAGCCAGCAAGAGTGGGGGAAAAAAGCACTGCAAAAAGTTGAGAATTTTCGCAAACCGa                                                                              <  1:232897/140‑1 (MQ=255)                                                      tGATGGTGCATGAACTGACGATGGACTCTCTTACCGAAAGCCTGGAATGGGCCGATGTGGTGGTGATTGGTCCCGGTCTGAGCCAGCAAGAGTGGGGGAAAAAAGCACTGCAAAAAGTTGAGAATTTTCGCAAACCGAtg                                                                            <  1:605840/140‑1 (MQ=255)                                                       gATGGTGCATGAACTGACGATGGACTCTCTTACCGAAAGCCTGGAATGGGCCGATGTGGTGGTGATTGGTCCCGGTCTGAGCCAGCAAGAGTGGGGGAAAAAAGCACTGCAAAAAGTTGAGAATTTTCGCAAACCGAtgt                                                                           <  1:720030/140‑1 (MQ=255)                                                            tGCATGAACTGACGATGGACTCTCTTACCGAAAGCCTGGAATGGGCCGATGTGGTGGTGATTGGTCCCGGTCTGAGCCAGCAAGAGTGGGGGAAAAAAGCACTGCAAAAAGTTGAGAATTTTCGCAAACCGATGTTGTgg                                                                      <  1:138439/140‑1 (MQ=255)                                                             gCATGAACTGACGATGGACTCTCTTACCGAAAGCCTGGAATGGGCCGATGTGGTGGTGATTGGTCCCGGTCTGAGCCAGCAAGTGTGGGGGAAAAAAGCACTGCAAAAAGTTGAGAATTTTCGCAAACCGATGTTGTggg                                                                     >  1:131652/1‑140 (MQ=255)                                                             gCATGAACTGACGATGGACTCTCTTACCGAAAGCCTGGAATGGGCCGATGTGGTGGTGATTGGTCCCGGTCTGAGCCAGCAAGAGTGGGGGAAAAAAGCACTGCAAAAAGTTGAGAATTTTCGCAAACCGATGTTGTggg                                                                     >  1:144546/1‑140 (MQ=255)                                                             gCATGAACTGACGATGGACTCTCTTACCGAAAGCCTGGAATGGGCCGATGTGGTGGTGATTAGTCCCGGTCTGAGCCAGCAAGAGTGGGGGAAAAAAGCACTGCAAAAAGTTGAGAATTTTCGCAAACCGATGTTGTggg                                                                     >  2:866879/1‑140 (MQ=255)                                                              cATGAACTGACGATGGACTCTCTTACCGAAAGCCTGGAATGGGCCGATGTGGTGGTGATTGGTCCCGGTCTGAGCCAGCAAGAGTGGGGGAAAAAAGCACTGCAAAAAGTTGAGAATTTTCGCAAACCGATGTTGTGGGa                                                                    >  2:526160/1‑140 (MQ=255)                                                                  aaCTGACGATGGACTCTCTTACCGAAAGCCTGGAATGGGCCGATGTGGTGGTGATTGGTCCCGGTCTGAGCCAGCAAGAGTGGGGGAAAAAAGCACTGCAAAAAGTTGAGAATTTTCGCAAACCGATGTTGTGGGATGcc                                                                >  2:356369/1‑140 (MQ=255)                                                                   aCTGACGATGGACTCTCTTACCGAAAGCCTGGAATGGGCCGATGTGGTGGTGATTGGTCCCGGTCTGAGCCAGCAAGAGTGGGGGAAAAAAGCACTGCAAAAAGTTGAGAATTTTCGCAAACCGATGTTGTGGGATGCCg                                                               <  1:526160/140‑1 (MQ=255)                                                                   aCTGACGATGGACTCTCTTACCGAAAGCCTGGAATGGGCCGATGTGGTGGTGATTGGTCCCGGTCTGAGCCAGCAAGAGTGGGGGAAAAAAGCACTGCAAAAAGTTGAGAATTTTCGCAAACCGATGTTGTGGGATGCCg                                                               <  2:649136/140‑1 (MQ=255)                                                                      gACGATGGACTCTCTTACCGAAAGCCTGGAATGGGCCGATGTGGTGGTGATTGGTCCCGGTCTGAGCCAGCAAGAGTGGGGGAAAAAAGCACTGCAAAAAGTTGAGAATTTTCGCAAACCGATGTTGTGGGa                                                                    >  1:578298/1‑132 (MQ=255)                                                                      gACGATGGACTCTCTTACCGAAAGCCTGGAATGGGCCGATGTGGTGGTGATTGGTCCCGGTCTGAGCCAGCAAGAGTGGGGGAAAAAAGCACTGCAAAAAGTTGAGAATTTTCGCAAACCGATGTTGTGGGa                                                                    <  2:578298/132‑1 (MQ=255)                                                                      gACGATGGACTCTCTTACCGAAAGCCTGGAATGGGCCGATGTGGTGGTGATTGGTCCCGGTCTGAGCCAGCAAGAGTGGGGGAAAAAAGCACTGCAAAAAGTTGAGAATTTTCGCAAACCGATGTTGTGGGATGCCGa                                                              >  2:362912/1‑138 (MQ=255)                                                                      gACGATGGACTCTCTTACCGAAAGCCTGGAATGGGCCGATGTGGTGGTGATTGGTCCCGGTCTGAGCCAGCAAGAGTGGGGGAAAAAAGCACTGCAAAAAGTTGAGAATTTTCGCAAACCGATGTTGTGGGATGCCGa                                                              <  1:362912/138‑1 (MQ=255)                                                                      gACGATGGACTCTCTTACCGAAAGCCTGGAATGGGCCGATGTGGTGGTGATTGGTCCCGGTCTGAGCCAGCAAGAGTGGGGGAAAAAAGCACTGCAAAAAGTTGAGAATTTTCGCAAACCGATGTTGTGGGATGCCGATg                                                            >  1:586628/1‑140 (MQ=255)                                                                      gACGATGGACTCTCTTACCGAAAGCCTGGAATGGGCCGATGTGGTGGTGATTGGTCCCGGTCTGAGCCAGCAAGAGTGGGGGAAAAAAGCACTGCAAAAAGTTGAGAATTTTCGCAAACCGATGTTGTGGGATGCCGATg                                                            <  1:356369/140‑1 (MQ=255)                                                                             gACTCTCTTACCGAAAGCCTGGAATGGGCCGATGTGGTGGTGATTGGTCCCGGTCTGAGCCAGCAAGAGTGGGGGAAAAAAGCACTGCAAAAAGTTGAGAATTTTCGCAAACCGATGTTGTGGGATGCCGATGCATTGaa                                                     >  1:261240/1‑140 (MQ=255)                                                                                 ctctTACCGAAAGCCTGGAATGGGCCGATGTGGTGGTGATTGGTCCCGGTCTGAGCCAGCAAGAGTGGGGGAAAAAAGCACTGCAAAAAGTTGAGAATTTTCGCAAACCGATGTTGTGGGATGCCGATGCATTGAACctg                                                 >  2:336132/1‑140 (MQ=255)                                                                                       ccGAAAGCCTGGAATGGGCCGATGTGGTGGTGATTGGTCCCGGTCTGAGCCAGCAAGAGTGGGGGAAAAAAGCACTGCAAAAAGTTGAGAATTTTCGCAAACCGATGTTGTGGGATGCCGATGCATTGAACCTGCTGGca                                           <  1:355874/140‑1 (MQ=255)                                                                                         gAAAGCCTGGAATGGGCCGATGTGGTGGTGATTGGTCCCGGTCTGAGCCAGCAAGAGTGGGGGAAAAAAGCACTGCAAAAAGTTGAGAATTTTCGCAAACCGATGTTGTGGGATGCCGATGCATTGAACCTGCTGGcaat                                         <  1:210273/140‑1 (MQ=255)                                                                                         gAAAGCCTGGAATGGGCCGATGTGGTGGTGATTGGTCCCGGTCTGAGCCAGCAAGAGTGGGGGAAAAAAGCACTGCAAAAAGTTGAGAATTTTCGCAAACCGATGTTGTGGGATGCCGATGCATTGAACCTGCTGGcaat                                         <  2:687169/140‑1 (MQ=255)                                                                                            aGCCTGGAATGGGCCGATGTGGTGGTGATTGGTCCCGGTCTGAGCCAGCAAGAGTGGGGGAAAAAAGCACTGCAAAAAGTTGAGAATTTTCGCAAACCGATGTTGTGGGATGCCGATGCATTGAACCTGCTGGcaatcaa                                      >  1:647604/1‑140 (MQ=255)                                                                                            aGCCTGGAATGGGCCGATGTGGTGGTGATTGGTCCCGGTCTGAGCCAGCAAGAGTGGGGGAAAAAAGCACTGCAAAAAGTTGAGAATTTTCGCAAACCGATGTTGTGGGATGCCGATGCATTGAACCTGCTGGcaat                                         >  1:802839/1‑137 (MQ=255)                                                                                            aGCCTGGAATGGGCCGATGTGGTGGTGATTGGTCCCGGTCTGAGCCAGCAAGAGTGGGGGAAAAAAGCACTGCAAAAAGTTGAGAATTTTCGCAAACCGATGTTGTGGGATGCCGATGCATTGAACCTGCTGGcaat                                         <  2:802839/137‑1 (MQ=255)                                                                                                    aTGGGCCGATGTGGTGGTGATTGGTCCCGGTCTGAGCCAGCAAGTGTGGGGGAAAAAAGCACTGCAAAAAGTTGAGAATTTTCGCAAACCGATGTTGTGGGATGCCGATGCATTGAACCTGCTGGCAATCAATCCCGATa                              <  2:131652/140‑1 (MQ=255)                                                                                                    aTGGGCCGATGTGGTGGTGATTGGTCCCGGTCTGAGCCAGCAAGAGTGGGGGAAAAAAGCACTGCAAAAAGTTGAGAATTTTCGCAAACCGATGTTGTGGGATGCCGATGCATTGAACCTGCTGGCAATCAATCCCGATa                              <  1:336132/140‑1 (MQ=255)                                                                                                       ggCCGATGTGGTGGTGATTGGTCCCGGTCTGAGCCAGCAAGAGTGGGGGAAAAAAGCACTGCAAAAAGTTGAGAATTTTCGCAAACCGATGTTGTGGGATGCCGATGCATTGAACCTGCTGGcaat                                         <  1:772276/126‑1 (MQ=255)                                                                                                       ggCCGATGTGGTGGTGATTGGTCCCGGTCTGAGCCAGCAAGAGTGGGGGAAAAAAGCACTGCAAAAAGTTGAGAATTTTCGCAAACCGATGTTGTGGGATGCCGATGCATTGAACCTGCTGGcaat                                         >  2:772276/1‑126 (MQ=255)                                                                                                        gCCGATGTGGTGGTGATTGGTCCCGGTCTGAGCCAGCAAGAGTGGGGGAAAAAAGCACTGCAAAAAGTTGAGAATTTTCGCAAACCGATGTTGTGGGATGCCGATGCATTGAACCTGCTGGCAATCAATCCCGATAAGCg                          >  1:130561/1‑140 (MQ=255)                                                                                                           gATGTGGTGGTGATTGGTCCCGGTCTGAGCCAGCAAGAGTGGGGGAAAAAAGCACTGCAAAAAGTTGAGAATTTTCGCAAACCGATGTTGTGGGATGCCGATGCATTGAACCTGCTGGCAATCAATCCCGATAAGCGTca                       <  2:144546/140‑1 (MQ=255)                                                                                                                       aTTGGTCCCGGTCTGAGCCAGCAAGAGTGGGGGAAAAAAGCACTGCAAAAAGTTGAGAATTTTCGCAAACCGATGTTGTGGGATGCCGATGCATTGAACCTGCTGGCAATCAATCCCGATAAGCGTCACAATCGCGTGAt           >  1:945704/1‑140 (MQ=255)                                                                                                                       aTTGGTCCCGGTCTGAGCCAGCAAGAGTGGGGGAAAAAAGCACTGCAAAAAGTTGAGAATTTTCGCAAACCGATGTTGTGGGATGCCGATGCATTGAACCTGCTGGCAATCAATCCCGATAAGCGTCACAATCGCGTGAt           <  2:261240/140‑1 (MQ=255)                                                                                                                        ttGGTCCCGGTCTGAGCCAGCAAGAGTGGGGGAAAAAAGCACTGCAAAAAGTTGAGAATTTTCGCAAACCGATGTTGTGGGATGCCGATGCATTGAACCTGCTGGCAATCAATCCCGATAAGCGTCACAATCGCGTGATc          <  1:866879/140‑1 (MQ=255)                                                                                                                           gTCCCGGTCTGAGCCAGCAAGAGTGGGGGAAAAAAGCACTGCAAAAAGTTGAGAATTTTCGCAAACCGATGTTGTGGGATGCCGATGCATTGAACCTGCTGGCAATCAATCCCGATAAGCGTCACAATCACGTGATCAcg       >  2:378173/1‑140 (MQ=255)                                                                                                                            tCCCGGTCTGAGCCAGCAAGAGTGGGGGAAAAAAGCACTGCAAAAAGTTGAGAATTTTCGCAAACCGATGTTGTGGGATGCCGATGCATTGAACCTGCTGGCAATCAATCCCGATAAGCGTCACAATCGCGTGATCAcgc      >  1:269575/1‑140 (MQ=255)                                                                                                                            tCCCGGTCTGAGCCAGCAAGAGTGGGGGAAAAAAGCACTGCAAAAAGTTGAGAATTTTCGCAAACCGATGTTGTGGGATGCCGATGCATTGAACCTGCTGGCAATCAATCCCGATAAGCGTCACAATCGCGTGATCAcgc      >  2:16446/1‑140 (MQ=255)                                                                                                                                ggTCTGAGCCAGCAAGAGTGGGGGAAAAAAGCACTGCGAAAAGTTGAGAATTTTCGCAAACCGATGTTGTGGGATGCCGATGCATTGAACCTGCTGGCAATCAATCCCGATAAGCGTCACAATCGCGTGATCACGCCGCa  >  2:332585/1‑140 (MQ=255)                                                                                                                                      |                                                                                                                                      AGTACTGACCCGCAGTGAAAACATTGCGCCGCTGCTGACTGCACGACCGGAATTGATGGTGCATGAACTGACGATGGACTCTCTTACCGAAAGCCTGGAATGGGCCGATGTGGTGGTGATTGGTCCCGGTCTGGGCCAGCAAGAGTGGGGGAAAAAAGCACTGCAAAAAGTTGAGAATTTTCGCAAACCGATGTTGTGGGATGCCGATGCATTGAACCTGCTGGCAATCAATCCCGATAAGCGTCACAATCGCGTGATCACGCCGCA  >  NC_000913/4394923‑4395189

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑