Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	517,337	T→C	V252A (GTG→GCG)	fetB →	putative iron ABC exporter membrane subunit FetB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	517,337	0	T	C	100.0%	143.2 / NA	40	V252A (GTG→GCG)	fetB	putative iron ABC exporter membrane subunit FetB
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (21/19);  total (21/19)

TTGATCCGGTGAAGGCGATTAAATATCAGATTATGGTGACCTTTATGCTGCTCTCAACCGCCAGCTTGTCGACCATTATTGCCTGCTATTTAACCTATCGTAAGTTTTATAATTCGCGCCACCAGTTGGTGGTGACGCAATTGAAGAAGAAATGATGCGATGCCGGATGCGCAGCATCGCATCCGGCGTTGTGGTTCATGTGCCGATCAACGGAATATCAATACAACAATGCATACAGCTGGCGGCGATACTTC  >  NC_000913/517208‑517461                                                                                                                                  |                                                                                                                             ttGATCCGGTGAAGGCGATTAAATATCAGATTATGGTGACCTTTATGCTGCTCTCAACCGCCAGCTTGTCGACCATTATTGCCTGCTATTTAACCTATCGTAAGTTTTATAATTCGCGCCACCAGTTGGCGGTGACGCaa                                                                                                                    <  1:13137/140‑1 (MQ=255)         gTGAAGGCGATTAAATATCAGATTATGGTGACCTTTATGCTGCTCTCAACCGCCAGCTTGTCGACCATTATTGCCTGCTATTTAACCTATCGTAAGTTTTATAATTCGCGCCACCAGTTGGCGGTGACGCAATTgaagaa                                                                                                            >  1:12138/1‑140 (MQ=255)         gTGAAGGCGATTAAATATCAGATTATGGTGACCTTTATGCTGCTCTCAACCGCCAGCTTGTCGACCATTATTGCCTGCTATTTAACCTATCGTAAGTTTTATAATTCGCGCCACCAGTTGGCGGTGACGCAATTgaagaa                                                                                                            >  1:900824/1‑140 (MQ=255)         gTGAAGGCGATTAAATATCAGATTATGGTGACCTTTATGCTGCTCTCAACCGCCAGCTTGTCGACCATTATTGCCTGCTATTTAACCTATCGTAAGTTTTATAATTCGCGCCACCAGTTGGCGGTGACGCAATTgaagaa                                                                                                            >  1:418591/1‑140 (MQ=255)                 gATTAAATATCAGATTATGGTGACCTTTATGCTGCTCTCAACCGCCAGCTTGTCGACCATTATTGCCTGCTATTTAACCTATCGTAAGTTTTATAATTCGCGCCACCAGTTGGCGGTGACGCAATTGAAGAAGAAatgat                                                                                                    <  1:876237/140‑1 (MQ=255)                                   ggTGACCTTTATGCTGCTCTCAACCGCCAGCTTGTCGACCATTATTGCCTGCTATTTAACCTATCGTAAGTTTTATAATTCGCGCCACCAGTTGGCGGTGACGCAATTGAAGAAGAAATGATGCGATGCCGGATGCgcag                                                                                  >  2:767508/1‑140 (MQ=255)                                            tATGCTGCTCTCAACCGCCAGCTTGTCGACCATTATTGCCTGCTATTTAACCTATCGTAAGTTTTATAATTCGCGCCACCAGTTGGCGGTGACGCAATTGAAGAAGAAATGATGCGATGCCGGATGCGCAgcatcgcatc                                                                         >  2:546749/1‑140 (MQ=255)                                                  gctCTCAACCGCCAGCTTGTCGACCATTATTGCCTGCTATTTAACCTATCGTAAGTTTTATAATTCGCGCCACCAGTTGGCGGTGACGCAATTGAAGAAGAAATGATGCGATGCCGGATGCGCAGCATCGCATCCGGCGt                                                                   <  2:141600/140‑1 (MQ=255)                                                  gctCTCAACCGCCAGCTTGTCGACCATTATTGCCTGCTATTTAACCTATCGTAAGTTTTATAATTCGCGCCACCAGTTGGCGGTGACGCAATTGAAGAAGAAATGATGCGATGCCGGATGCGCAGCATCGCATCCGGCGt                                                                   <  2:12138/140‑1 (MQ=255)                                                   ctctCAACCGCCAGCTTGTCGACCATTATTGCCTGCTATTTAACCTATCGTAAGTTTTATAATTCGCGCCACCAGTTGGCGGTGACGCAATTGAAGAAGAAATGATGCGATGCCGGATGCGCAGCATCGCATCCGGCGtt                                                                  >  2:788625/1‑140 (MQ=255)                                                   ctctCAACCGCCAGCTTGTCGACCATTATTGCCTGCTATTTAACCTATCGTAAGTTTTATAATTCGCGCCACCAGTTGGCGGTGACGCAATTGAAGAAGAAATGATGCGATGCCGGATGCGCAGCATCGCATCCGGCGtt                                                                  >  2:927546/1‑140 (MQ=255)                                                     ctcAACCGCCAGCTTGTCGACCATTATTGCCTGCTATTTAACCTATCGTAAGTTTTATAATTCGCGCCACCAGTTGGCGGTGACGCAATTGAAGAAGAAATGATGCGATGCCGGATGCGCAGCATCGCATCCGGCGTtgt                                                                <  1:600701/140‑1 (MQ=255)                                                          ccGCCAGCTTGTCGACCATTATTGCCTGCTATTTAACCTATCGTAAGTTTTATAATTCGCGCCACCAGTTGGCGGTGACGCAATTGAAGAAGAAATGATGCGATGCCGGATGCGCAGCATCGCATCCGGCGTTGTGGTTc                                                           <  1:788625/140‑1 (MQ=255)                                                             ccAGCTTGTCGACCATTATTGCCTGCTATTTAACCTATCGTAAGTTTTATAATTCGCGCCACCAGTTGGCGGTGACGCAATTGAAGAAGAAATGATGCGATGCCGGATGCGCAGCATCGCATCCGGCGTTGTGGTTCAtg                                                        >  1:6312/1‑140 (MQ=255)                                                             ccAGCTTGTCGACCATTATTGCCTGCTATTTAACCTATCGTAAGTTTTATAATTCGCGCCACCAGTTGGCGGTGACGCAATTGAAGAAGAAATGATGCGATGCCGGATGCGCAGCATCGCATCCGGCGTTGTGGTTCAtg                                                        >  2:279042/1‑140 (MQ=255)                                                                       gACCATTATTGCCTGCTATTTAACCTATCGTAAGTTTTATAATTCGCGCCACCAGTTGGCGGTGACGCAATTGAAGAAGAAATGATGCGATGCCGGATGCGCAGCATCGCATCCGGCGTTGTGGTTCATGTGCCGATCaa                                              <  2:900824/140‑1 (MQ=255)                                                                            ttattGCCTGCTATTTAACCTATCGTAAGTTTTATAATTCGCGCCACCAGTTGGCGGTGACGCAATTGAAGAAGAAATGATGCGATGCCGGATGCGCAGCATCGCATCCGGCGTTGTGGTTCATGTGCCGATCAACGGaa                                         >  1:749532/1‑140 (MQ=255)                                                                             tattGCCTGCTATTTAACCTATCGTAAGTTTTATAATTCGCGCCACCAGTTGGCGGTGACGCAATTGAAGAAGAAATGATGCGATGCCGGATGCGCAGCATCGCATCCGGCGTTGTGGTTCATGTGCCGATCAACGGAat                                        <  1:546749/140‑1 (MQ=255)                                                                               ttGCCTGCTATTTAACCTATCGTAAGTTTTATAATTCGCGCCACCAGTTGGCGGTGACGCAATTGAAGAAGAAATGATGCGATGCCGGATGCGCAGCATCGCATCCGGCGTTGTGGTTCATGTGCCGATCAACGGAatat                                      <  2:6312/140‑1 (MQ=255)                                                                               ttGCCTGCTATTTAACCTATCGTAAGTTTTATAATTCGCGCCACCAGTTGGCGGTGACGCAATTGAAGAAGAAATGATGCGATGCCGGATGCGCAGCATCGCATCCGGCGTTGTGGTTCATGTGCCGATCAACGGAatat                                      <  1:958446/140‑1 (MQ=255)                                                                               ttGCCTGCTATTTAACCTATCGTAAGTTTTATAATTCGCGCCACCAGTTGGCGGTGACGCAATTGAAGAAGAAATGATGCGATGCCGGATGCGCAGCATCGCATCCGGCGTTGTGGTTCATGTGCCGATCAACGGAatat                                      <  1:23307/140‑1 (MQ=255)                                                                                  ccTGCTATTTAACCTATCGTAAGTTTTATAATTCGCGCCACCAGTTGGCGGTGACGCAATTGAAGAAGAAATGATGCGATGCCGGATGCGCAGCATCGCATCCGGCGTTGTGGTTCATGTGCCGATCAACGGAATATCaa                                   >  2:816423/1‑140 (MQ=255)                                                                                   cTGCTATTTAACCTATCGTAAGTTTTATAATTCGCGCCACCAGTTGGCGGTGACGCAATTGAAGAAGAAATGATGCGATGCCGGATGCGCAGCATCGCATCCGGCGTTGTGGTTCATGTGCCGATCAACGGAATATCAAt                                  >  2:314977/1‑140 (MQ=255)                                                                                    tGCTATTTAACCTATCGTAAGTTTTATAATTCGCGCCACCAGTTGGCGGTGACGCAATTGAAGAAGAAATGATGCGATGCCGGATGCGCAGCATCGCATCCGGCGTTGTGGTTCATGTGCCGATCAACGGAATATCAATa                                 <  1:927546/140‑1 (MQ=255)                                                                                      cTATTTAACCTATCGTAAGTTTTATAATTCGCGCCACCAGTTGGCGGTGACGCAATTGAAGAAGAAATGATGCGATGCCGGATGCGCGGCATCGCATCCGGCGTTGTGGTTCATGTGCCGATCAACGGAATATCAATaca                               >  1:940935/1‑140 (MQ=255)                                                                                      cTATTTAACCTATCGTAAGTTTTATAATTCGCGCCACCAGTTGGCGGTGACGCAATTGAAGAAGAAATGATGCGATGCCGGATGCGCAGCATCGCATCCGGCGTTGTGGTTCATGTGCCGATCAACGGAATATCAATaca                               <  1:314977/140‑1 (MQ=255)                                                                                      cTATTTAACCTATCGTAAGTTTTATAATTCGCGCCACCAGTTGGCGGTGACGCAATTGAAGAAGAAATGATGCGATGCCGGATGCGCAGCATCGCATCCGGCGTTGTGGTTCATGTGCCGATCAACGGAATATCAATaca                               >  1:428644/1‑140 (MQ=255)                                                                                       tATTTAACCTATCGTAAGTTTTATAATTCGCGCCACCAGTTGGCGGTGACGCAATTGAAGAAGAAATGATGCGATGCCGGATGCGCAGCATCGCATCCGGCGTTGTGGTTCATGTGCCGATCAACGGAATATCAATaca                               <  1:763720/139‑1 (MQ=255)                                                                                       tATTTAACCTATCGTAAGTTTTATAATTCGCGCCACCAGTTGGCGGTGACGCAATTGAAGAAGAAATGATGCGATGCCGGATGCGCAGCATCGCATCCGGCGTTGTGGTTCATGTGCCGATCAACGGAATATCAATaca                               >  2:763720/1‑139 (MQ=255)                                                                                         tttAACCTATCGTAAGTTTTATAATTCGCGCCACCAGTTGGCGGTGACGCAATTGAAGAAGAAATGATGCGATGCCGGATGCGCGGCATCGCATCCGGCGTTGTGGTTCATGTGCCGATCAACGGAATATCAATacaaca                            <  2:940935/140‑1 (MQ=255)                                                                                           tAACCTATCGTAAGTTTTATAATTCGCGCCACCAGTTGGCGGTGACGCAATTGAAGAAGAAATGATGCGATGCCGGATGCGCAGCATCGCATCCGGCGTTGTGGTTCATGTGCCGATCAACGGAATATCAATACAACAAt                          <  2:749532/140‑1 (MQ=255)                                                                                             aCCTATCGTAAGTTTTATAATTCGCGCCACCAGTTGGCGGTGACGCAATTGAAGAAGAAATGATGCGATGCCGGATGCGCAGCATCGCATCCGGCGTTGTGGTTCATGTGCCGATCAACGGAATATCAATACAACAATGc                        <  2:418591/140‑1 (MQ=255)                                                                                                 aTCGTAAGTTTTATAATTCGCGCCACCAGTTGGCGGTGACGCAATTGAAGAAGAAATGATGCGATGCCGGATGCGCAGCATCGCATCCGGCGTTGTGGTTCATGTGCCGATCAACGGAATATCAATa                                 >  1:194421/1‑127 (MQ=255)                                                                                                 aTCGTAAGTTTTATAATTCGCGCCACCAGTTGGCGGTGACGCAATTGAAGAAGAAATGATGCGATGCCGGATGCGCAGCATCGCATCCGGCGTTGTGGTTCATGTGCCGATCAACGGAATATCAATa                                 <  2:194421/127‑1 (MQ=255)                                                                                                 aTCGTAAGTTTTATAATTCGCGCCACCAGTTGGCGGTGACGCAATTGAAGAAGAAATGATGCGATGCCGGATGCGCAGCATCGCATCCGGCGTTGTGGTTCATGTGCCGATCAACGGAATATCAATACAACAATGCATAc                    >  1:526302/1‑140 (MQ=255)                                                                                                   cGTAAGTTTTATAATTCGCGCCACCAGTTGGCGGTGACGCAATTGAAGAAGAAATGATGCGATGCCGGATGCGCAGCATCGCATCCGGCGTTGTGGTTCATGTGCCGATCAACGGAATATCAATACAACAATGCATACAg                  >  1:82396/1‑140 (MQ=255)                                                                                                            tataATTCGCGCCACCAGTTGGCGGTGACGCAATTGAAGAAGAAATGATGCGATGCCGGATGCGCAGCATCGCATCCGGCGTTGTGGTTCATGTGCCGATCAACGGAATATCAATACAACAATGCATACAGCTGgcggcg         >  1:51868/1‑140 (MQ=255)                                                                                                            tataATTCGCGCCACCAGTTGGCGGTGACGCAATTGAAGAAGAAATGATGCGATGCCGGATGCGCAGCATCGCATCCGGCGTTGTGGTTCATGTGCCGATCAACGGAATATCAATACAACAATGCATACAGCTGgcggcg         >  2:430804/1‑140 (MQ=255)                                                                                                                aTTCGCGCCACCAGTTGGCGGTGACGCAATTGAAGAAGAAATGATGCGATGCCGGATGCGCAGCATCGCATCCGGCGTTGTGGTTCATGTGCCGATCAACGGAATATCAATACAACAATGCATACAGCTGGCGGCGATAc     <  2:428644/140‑1 (MQ=255)                                                                                                                   cgcgCCACCAGTTGGCGGTGACGCAATTGAAGAAGAAATGATGCGATGCCGGATGCGCAGCATCGCATCCGGCGTTGTGGTTCATGTGCCGATCAACGGAATATCAATACAACAATGCATACAGCTGGCGGCGATACTTc  >  1:967320/1‑140 (MQ=255)                                                                                                                                  |                                                                                                                             TTGATCCGGTGAAGGCGATTAAATATCAGATTATGGTGACCTTTATGCTGCTCTCAACCGCCAGCTTGTCGACCATTATTGCCTGCTATTTAACCTATCGTAAGTTTTATAATTCGCGCCACCAGTTGGTGGTGACGCAATTGAAGAAGAAATGATGCGATGCCGGATGCGCAGCATCGCATCCGGCGTTGTGGTTCATGTGCCGATCAACGGAATATCAATACAACAATGCATACAGCTGGCGGCGATACTTC  >  NC_000913/517208‑517461

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑