Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,453,419	A→G	L283L (CTA→CTG)	ytfT →	galactofuranose ABC transporter putative membrane subunit YtfT

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,453,419	0	A	G	100.0%	128.8 / NA	37	L283L (CTA→CTG)	ytfT	galactofuranose ABC transporter putative membrane subunit YtfT
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (12/25);  total (12/25)

TTGCGGGCATTATCGTGGCGGCGGATATTCGCGGTGCCGATGCCAACAACGCTGGGTTATGGCTGGAGCTGGACGCCATTCTCGCGGTGGTGATTGGCGGCGGATCGCTGATGGGCGGGCGCTTTAACCTGCTACTTTCGGTGGTGGGGGCGCTGATTATTCAGGGGATGAACACCGGAATTTTGCTTTCGGGCTTTCCGCCAGAGATGAACCAGGTGGTGAAAGCGGTGGTGGTGCTTTGCGTGCTGATTGTTCAGTCGC  >  NC_000913/4453286‑4453546                                                                                                                                      |                                                                                                                                ttGCGGGCATTATCGTGGCGGCGGATATTCGCGGTGCCGATGCCAACAACGCTGGGTTATGGCTGGAGCTGGACGCCATTCTCGCGGTGGTGATTGGCGGCGGATCGCTGATGGGCGGGCGCTTTAACCTGCTGCTTTc                                                                                                                            <  2:522873/139‑1 (MQ=255) ttGCGGGCATTATCGTGGCGGCGGATATTCGCGGTGCCGATGCCAACAACGCTGGGTTATGGCTGGAGCTGGACGCCATTCTCGCGGTGGTGATTGGCGGCGGATCGCTGATGGGCGGGCGCTTTAACCTGCTGCTTTc                                                                                                                            >  1:522873/1‑139 (MQ=255) ttGCGGGCATTATCGTGGCGGCGGATATTCGCGGTGCCGATGCCAACAACGCTGGGTTATGGCTGGAGCTGGACGCCATTCTCGCGGTGGTGATTGGCGGCGGATCGCTGATGGGCGGGCGCTTTAACCTGCTGCTTTCg                                                                                                                           <  1:243884/140‑1 (MQ=255)            aTCGTGGCGGCGGATATTCGCGGTGCCGATGCCAACAACGCTGGGTTATGGCTGGAGCTGGACGCCATTCTCGCGGTGGTGATTGGCGGCGGATCGCTGATGGGCGGGCGCTTTAACCTGCTGCTTTCGGTGGTGGGGgc                                                                                                                <  2:9998/140‑1 (MQ=255)            aTCGTGGCGGCGGATATTCGCGGTGCCGATGCCAACAACGCTGGGTTATGGCTGGAGCTGGACGCCATTCTCGCGGTGGTGATTGGCGGCGGATCGCTGATGGGCGGGCGCTTTAACCTGCTGCTTTCGGTGGTGGGGgc                                                                                                                <  2:52759/140‑1 (MQ=255)            aTCGTGGCGGCGGATATTCGCGGTGCCGATGCCAACAACGCTGGGTTATGGCTGGAGCTGGACGCCATTCTCGCGGTGGTGATTGGCGGCGGATCGCTGATGGGCGGGCGCTTTAACCTGCTGCTTTCGGTGGTGGGGgc                                                                                                                <  2:494310/140‑1 (MQ=255)               gTGGCGGCGGATATTCGCGGTGCCGATGCCAACAACGCTGGGTTATGGCTGGAGCTGGACGCCATTCTCGCGGTGGTGATTGGCGGCGGATCGCTGATGGGCGGGCGCTTTAACCTGCTGCTTTCGGTGGTGGGGGCGCt                                                                                                             <  2:47820/140‑1 (MQ=255)                  gcggcgGATATTCGCGGTGCCGATGCCAACAACGCTGGGTTATGGCTGGAGCTGGACGCCATTCTCGCGGTGGTGATTGGCGGCGGATCGCTGATGGGCGGGCGCTTTAACCTGCTGCTTTCGGTGGTGGGGGCGCTGat                                                                                                          >  1:66431/1‑140 (MQ=255)                  gcggcgGATATTCGCGGTGCCGATGCCAACAACGCTGGGTTATGGCTGGAGCTGGACGCCATTCTCGCGGTGGTGATTGGCGGCGGATCGCTGATGGGCGGGCGCTTTAACCTGCTGCTTTCGGTGGTGGGGGCGCTGat                                                                                                          <  2:700923/140‑1 (MQ=255)                       ggATATTCGCGGTGCCGATGCCAACAACGCTGGGTTATGGCTGGAGCTGGACGCCATTCTCGCGGTGGTGATTGGCGGCGGATCGCTGATGGGCGGGCGCTTTAACCTGCTGCTTTCGGTGGTGGGGGCGCTGATTATTc                                                                                                     <  2:528108/140‑1 (MQ=255)                       ggATATTCGCGGTGCCGATGCCAACAACGCTGGGTTATGGCTGGAGCTGGACGCCATTCTCGCGGTGGTGATTGGCGGCGGATCGCTGATGGGCGGGCGCTTTAACCTGCTGCTTTCGGTGGTGGGGGCGCTGATTATTc                                                                                                     <  1:18795/140‑1 (MQ=255)                       ggATATTCGCGGTGCCGATGCCAACAACGCTGGGTTATGGCTGGAGCTGGACGCCATTCTCGCGGTGGTGATTGGCGGCGGATCGCTGATGGGCGGGCGCTTTAACCTGCTGCTTTCGGTGGTGGGGGCGCTGATTATTc                                                                                                     <  2:398954/140‑1 (MQ=255)                        gATATTCGCGGTGCCGATGCCAACAACGCTGGGTTATGGCTGGAGCTGGACGCCATTCTCGCGGTGGTGATTGGCGGCGGATCGCTGATGGGCGGGCGCTTTAACCTGCTGCTTTCGGTGGTGGGGgcg                                                                                                               <  2:827432/129‑1 (MQ=255)                        gATATTCGCGGTGCCGATGCCAACAACGCTGGGTTATGGCTGGAGCTGGACGCCATTCTCGCGGTGGTGATTGGCGGCGGATCGCTGATGGGCGGGCGCTTTAACCTGCTGCTTTCGGTGGTGGGGgcg                                                                                                               >  1:827432/1‑129 (MQ=255)                                   tGCCGATGCCAACAACGCTGGGTTATGGCTGGAGCTGGACGCCATTCTCGCGGTGGTGATTGGCGGCGGATCGCTGATGGGCGGGCGCTTTAACCTGCTGCTTTCGGTGGTGGGGGCGCTGATTATTCAg                                                                                                   <  2:529906/130‑1 (MQ=255)                                   tGCCGATGCCAACAACGCTGGGTTATGGCTGGAGCTGGACGCCATTCTCGCGGTGGTGATTGGCGGCGGATCGCTGATGGGCGGGCGCTTTAACCTGCTGCTTTCGGTGGTGGGGGCGCTGATTATTCAg                                                                                                   >  1:529906/1‑130 (MQ=255)                                     ccGATGCCAACAACGCTGGGTTATGGCTGGAGCTGGACGCCATTCTCGCGGTGGTGATTGGCGGCGGATCGCTGATGGGCGGGCGCTTTAACCTGCTGCTTTCGGTGGTGGGGGCGCTGATTATTCAGGGGATGAACAcc                                                                                       >  2:702357/1‑140 (MQ=255)                                                    cTGGGTTATGGCTGGAGCTGGACGCCATTCTCGCGGTGGTGATTGGCGGCGGATCGCTGATGGGCGGGCGCTTTAACCTGCTGCTTTCGGTGGTGGGGGCGCTGATTATTCAGGGGATGAACACCGGAATTTTGCTTTCg                                                                        >  1:307727/1‑140 (MQ=255)                                                     tGGGTTATGGCTGGAGCTGGACGCCATTCTCGCGGTGGTGATTGGCGGTGGATCGCTGATGGGCGGGCGCTTTAACCTGCTGCTTTCGGTGGTGGGGGCGCTGATTATTCAGGGGATGAACACCGGAATTTTGCTTTCgg                                                                       >  2:57930/1‑140 (MQ=255)                                                      gggTTATGGCTGGAGCTGGACGCCATTCTCGCGGTGGTGATTGGCGGCGGATCTCTGATGGGCGGGCGCTTTAACCTGCTGCTTTCGGTGGTGGGGGCGCTGATTATTCAGGGGATGAACACCGGAATTTTGCTTTCggg                                                                      <  2:845284/140‑1 (MQ=255)                                                              gctggagctggaCGCCATTCTCGCGGTGGTGATTGGCGGCGGATCGCTGATGGGCGGGCGCTTTAACCTGCTGCTTTCGGTGGTGGGGGCGCTGATTATTCAGGGGATGAACACCGGAATTTTGCTTTCGGGCTTTCCGc                                                              <  1:370813/140‑1 (MQ=255)                                                               ctggagctggaCGCCATTCTCGCGGTGGTGATTGGCGGTGGATCGCTGATGGGCGGGCGCTTTAACCTGCTGCTTTCGGTGGTGGGGGCGCTGATTATTCAGGGGATGAACACCGGAATTTTGCTTTCGGGCTTTCCGcc                                                             <  1:57930/140‑1 (MQ=255)                                                                 ggagctggaCGCTATTCTCGCGGTGGTGATTGGCGGCGGATCGCTGATGGGCGGGCGCTTTAACCTGCTGCTTTCGGTGGTGGGGGCGCTGATTATTCAGGGGATGAACACCGGAATTTTGCTTTCGGGCTTTCCGCCag                                                           >  2:834203/1‑140 (MQ=255)                                                                 ggagctggaCGCCATTCTCGCGGTGGTGATTGGCGGCGGATCGCTGATGGGCGGGCGCTTTAACCTGCTGCTTTCGGTGGTGGGGGCGCTGATTATTCAGGGGATGAACACCGGAATTTTGCTTTCGGGCTTTCCGCCag                                                           >  2:122542/1‑140 (MQ=255)                                                                 ggagctggaCGCCATTCTCGCGGTGGGGATTGGCGGCGGATCGCTGATGGGCGGGCGCTTTAACCTGCTGCTTTCGGTGGTGGGGGCGGTGATTATTCAGGGGGTGAACACCGGAATTTTGGTTTCCGGCTTTCCGCCaa                                                           >  2:515321/1‑139 (MQ=255)                                                                  gagctggaCGCCATTCTCGCGGTGGTGATTGGCGGCGGATCGCTGATGGGCGGGCGCTTTAACCTGCTGCTTTCGGTGGTGGGGGCGCTGATTATTCAGGGGATGAACACCGGAATTTTGCTTTCGGGCTTTCCGCCaga                                                          >  2:503313/1‑140 (MQ=255)                                                                        gaCGCCATTCTCGCGGTGGTGATTGGCGGCGGATCGCTGATGGGCGGGCGCTTTAACCTGCTGCTTTCGGTGGTGGGGGCGCTGATTATTCAGGGGATGAACACCGGAATTTTGCTTTCGGGCTTTCCGCCAGAGATGaa                                                    >  1:281159/1‑140 (MQ=255)                                                                          cGCCATTCTCGCGGTGGTGATTGGCGGCGGATCGCTGATGGGCGGGCGCTTTAACCTGCTGCTTTCGGTGGTGGGGGCGCTGATTATTCAGGGGATGAACACCGGAATTTTGCTTTCGGGCTTTCCGCCAGAGATGAAcc                                                  <  1:173485/140‑1 (MQ=255)                                                                            ccATTCTCGCGGTGGTGATTGGCGGCGGATCGCTGATGGGCGGGCGCTTTAACCTGCTGCTTTCGGTGGTGGGGGCGCTGATTATTCAGGGGATGAACACCGGAATTTTGCTTTCGGGCTTTCCGCCAGAGATGAACCAg                                                <  2:234990/140‑1 (MQ=255)                                                                                         ggtgATTGGCGGCGGATCGCTGATGGGCGGGCGCTTTAACCTGCTGCTTTCGGTGGTGGGGGCGCTGATTATTCAGGGGATGAACACCGGAATTTTGCTTTCGGGCTTTCCGCCAGAGATGAACCAGGTGGTGAAAGCgg                                   <  1:503313/140‑1 (MQ=255)                                                                                                   ggcggATCGCTGATGGGCGGGCGCTTTAACCTGCTGCTTTCGGTGGTGGGGGCGCTGATTATTCAGGGGATGAACACCGGAATTTTGCTTTCGGGCTTTCCGCCAGAGATGAACCAGGTGGTGAAAGCGGTGGTGGTGCt                         <  2:281159/140‑1 (MQ=255)                                                                                                     cggATCGCTGATGGGCGGGCGCTTTAACCTGCTGCTTTCGGTGGTGGGGGCGCTGATTATTCAGGGGATGAACACCGGAATTTTGCTTTCGGGCTTTCCGCCAGAGATGAACCAGGTGGTGAAAGCGGTGGTGGTGCttt                       <  1:702357/140‑1 (MQ=255)                                                                                                              gATGGGCGGGCGCTTTAACCTGCTGCTTTCGGTGGTGGGGGCGCTGATTATTCAGGGGATGAACACCGGAATTTTGCTTTCGGGCTTTCCGCCAGAGATGAACCAGGTGGTGAAAGCGGTGGTGGTGCTTTGCGTGCTGa              <  1:834203/140‑1 (MQ=255)                                                                                                               aTGGGCGGGCGCTTTAACCTGCTGCTTTCGGTGGTGGGGGCGCTGATTATTCAGGGGATGAACACCGGAATTTTGCTTTCGGGCTTTCCGCCAGAGATGAACCAGGTGGTGAAAGCGGTGGTGGTGCTTTGCGTGCTGAt             <  1:122542/140‑1 (MQ=255)                                                                                                                  ggcgggcgCTTTAACCTGCTGCTTTCGGTGGTGGGGGCGCTGATTATTCAGGGGATGAACACCGGAATTTTGCTTTCGGGCTTTCCGCCAGAGATGAACCAGGTGGTGAAAGCGGTGGTGGTGCTTTGCGTGCTGATTGt          >  1:138572/1‑140 (MQ=255)                                                                                                                          cTTTAACCTGCTGCTTTCGGTGGTGGGGGCGCTGATTATTCAGGGGATGAACACCGGAATTTTGCTTTCGGGCTTTCCGCCAGAGATGAACCAGGTGGTGAAAGCGGTGGTGGTGCTTTGCGTGCTGATTGTTCAGTCGc  <  2:66431/140‑1 (MQ=255)                                                                                                                          cTTTAACCTGCTGCTTTCGGTGGTGGGGGCGCTGATTATTCAGGGGATGAACACCGGAATTTTGCTTTCGGGCTTTCCGCCAGAGATGAACCAGGTGGTGAAAGCGGTGGTGGTGCTTTGCGTGCTGATTGTTCAGTCGc  <  1:515321/140‑1 (MQ=255)                                                                                                                          cTTTAACCTGCTGCTTTCGGTGGTGGGGGCGCTGATTATTCAGGGGATGAACACCGGAATTTTGCTTTCGGGCTTTCCGCCAGAGATGAACCAGGTGGTGAAAGCGGTGGTGGTGCTTTGCGTGCTGATTGTTCAGTCGc  <  1:466498/140‑1 (MQ=255)                                                                                                                                      |                                                                                                                                TTGCGGGCATTATCGTGGCGGCGGATATTCGCGGTGCCGATGCCAACAACGCTGGGTTATGGCTGGAGCTGGACGCCATTCTCGCGGTGGTGATTGGCGGCGGATCGCTGATGGGCGGGCGCTTTAACCTGCTACTTTCGGTGGTGGGGGCGCTGATTATTCAGGGGATGAACACCGGAATTTTGCTTTCGGGCTTTCCGCCAGAGATGAACCAGGTGGTGAAAGCGGTGGTGGTGCTTTGCGTGCTGATTGTTCAGTCGC  >  NC_000913/4453286‑4453546

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑