Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,495,421	C→T	A263A (GCG→GCA)	ahr ←	aldehyde reductase, NADPH‑dependent

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,495,421	0	C	T	100.0%	114.6 / NA	36	A263A (GCG→GCA)	ahr	aldehyde reductase, NADPH‑dependent
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (21/15);  total (21/15)

GTGGTCGGCGCAACCTTGCTGCGGGCGGCAAAACGCATCAGCTTACGCAGCTCATAAGGCGTGCCGGTAGCAGAACCAGAGACGCTGCGATCGCCCGCAATTAACGTAAAGGCCGGAACAGACAGCGGCGTGAGAACCGCACCGACCGTATGGAAATT‑ACCGCCATAGGTCAGCGCCTCAAAATAGGGCTGCCAGTCGAGGCTGACGTTGACGGTGTTGATAATGAGATCAAACTGCCCCGCCAGTGCTTTCAGTGCCTGCGGATCGC  >  NC_000913/4495284‑4495551                                                                                                                                          |                                                                                                                                    gtggtCGGCGCAACCTTGCTGCGGGCGGCAAAACGCATCAGCTTACGCAGCTCATAAGGCGTGCCGGTAGCAGAACCAGAGACGCTGCGATCGCCCGCAATTAACGTAAAGGCCGGAACAGACAGCGGCGTGAGAACTGc                                                                                                                                   >  2:814608/1‑140 (MQ=255)  tggtCGGCGCAACCTTGCTGCGGGCGGCAAAACGCATCAGCTTACGCAGCTCATAAGGCGTGCCGGTAGCAGAACCAGAGACGCTGCGATCGCCCGCAATTAACGTAAAGGCCGGAACAGACAGCGGCGTGAGAACTGCa                                                                                                                                  >  1:102205/1‑140 (MQ=255)  tggtCGGCGCAACCTTGCTGCGGGCGGCAAAACGCATCAGCTTACGCAGCTCATAAGGCGTGCCGGTAGCAGAACCAGAGACGCTGCGATCGCCCGCAATTAACGTAAAGGCCGGAACAGACAGCGGCGTGAGAACTGCa                                                                                                                                  >  1:221697/1‑140 (MQ=255)  tggtCGGCGCAACCTTGCTGCGGGCGGCAAAACGCATCAGCTTACGCAGCTCATAAGGCGTGCCGGTAGCAGAACCAGAGACGCTGCGATCGCCCGCAATTAACGTAAAGGCCGGAACAGACAGCGGCGTGAGAACTGCa                                                                                                                                  >  1:420341/1‑140 (MQ=255)      cGGCGCAACCTTGCTGCGGGCGGCAAAACGCATCAGCTTACGCAGCTCATAAGGCGTGCCGGTAGCAGAACCAGAGACGCTGCGATCGCCCGCAATTAACGTAAAGGCCGGAACAGACAGCGGCGTGAGAACTGCaccga                                                                                                                              <  2:215501/140‑1 (MQ=255)       ggCGCAACCTTGCTGCGGGCGGCAAAACGCATCAGCTTACGCAGCTCATAAGGCGTGCCGGTAGCAGAACCAGAGACGCTGCGATCGCCCGCAATTAACGTAAAGGCCGGAACAGACAGCGGCGTGAGAACTGCaccgac                                                                                                                             >  1:875098/1‑140 (MQ=255)       ggCGCAACCTTGCTGCGGGCGGCAAAACGCATCAGCTTACGCAGCTCATAAGGCGTGCCGGTAGCAGAACCAGAGACGCTGCGATCGCCCGCAATTAACGTAAAGGCCGGAACAGACAGCGGCGTGAGAACTGCaccgac                                                                                                                             >  2:699156/1‑140 (MQ=255)        gcgcAACCTTGCTGCGGGCGGCAAAACGCATCAGCTTACGCAGCTCATAAGGCGTGCCGGTAGCAGAACCAGAGACGCTGCGATCGCCCGCAATTAACGTAAAGGCCGGAACAGACAGCGGCGTGAGAACTGCaccgacc                                                                                                                            <  2:102205/140‑1 (MQ=255)                    tgcGGGCGGCAAAACGCATCAGCTTACGCAGCTCATAAGGCGTGCCGGTAGCAGAACCAGAGACGCTGCGATCGCCCGCAATTAACGTAAAGGCCGGAACAGACAGCGGCGTGAGAACTGCACCGACCGTATGGAAATT‑a                                                                                                               <  2:875098/140‑1 (MQ=255)                    tgcGGGCGGCAAAACGCATCAGCTTACGCAGCTCATAAGGCGTGCCGGTAGCAGAACCAGAGACGCTGCGATCGCCCGCAATTAACGTAAAGGCCGGAACAGACAGCGGCGTGAGAACTGCACCGACCGTATGGAAATT‑a                                                                                                               <  1:699156/140‑1 (MQ=255)                        ggcggcAAAACGCATCAGCTTACGCAGCTCATAAGGCGTGCCGGTAGCAGAACCAGAGACGCTGCGATCGCCCGCAATTAACGTAAAGGCCGGAACAGACAGCGGCGTGAGAACTGCACCGACCGTATGGAAATT‑ACCGc                                                                                                           >  2:460545/1‑140 (MQ=255)                               aaaCGCATCAGCTTACGCAGCTCATAAGGCGTGCCGGTAGCAGAACCAGAGACGCTGCGATCGCCCGCAATTAACGTAAAGGCCGGAACAGACAGCGGCGTGAGAACTGCACCGACCGTATGGAAATT‑ACCGCCATAGGt                                                                                                    <  1:460545/140‑1 (MQ=255)                               aaaCGCATCAGCTTACGCAGCTCATAAGGCGTGCCGGTAGCAGAACCAGAGACGCTGCGATCGCCCGCAATTAACGTAAAGGCCGGAACAGACAGCGGCGTGAGAACTGCACCGACCGTATGGAAATT‑ACCGCCATAGGt                                                                                                    <  1:814608/140‑1 (MQ=255)                                       cAGCTAACGCAGCTCATAGGGCGTGCCGGTAGGGAACCCAGGGACGCTGCGATCGCCCGCAATAACCGAAAAGGCCGGAACAGACAGCGGCGTGAAAACTCCCCCGACCGTATGGAAATT‑ACCGCCATAGGTCAGCGCCt                                                                                            <  2:253411/140‑1 (MQ=255)                                                   cTCATAAGGCGTGCCGGTAGCAGAACCAGAGACGCTGCGATCGCCCGCAATTAACGTAAAGGCCGGAACAGACAGCGGCGTGAGAACTGCACCGACCGTATGGAAATT‑ACCGCCATAGGTCAGCGCCTCAAAATAGGGCt                                                                                >  1:372046/1‑140 (MQ=255)                                                        aaGGCGTGCCGGTAGCAGAACCAGAGACGCTGCGATCGCCCGCAATTAACGTAAAGGCCGGAACAGACAGCGGCGTGAGAACTGCACCGACCGTATGGAAATT‑ACCGCCATAGGTCAGCGCCTCAAAATAGGGCTGCCAg                                                                           >  1:522215/1‑140 (MQ=255)                                                             gTGCCGGTAGCAGAACCAGAGACGCTGCGATCGCCCGCAATTAACGTAAAGGCCGGAACAGACAGCGGCGTGAGAACTGCACCGACCGTATGGAAATT‑ACCGCCATAGGTCAGCGCCTCAAAATAGGGCTGCCAGTCGAg                                                                      >  1:709026/1‑140 (MQ=255)                                                             gTGCCGGTAGCAGAACCAGAGACGCTGCGATCGCCCGCAATTAACGTAAAGGCCGGAACAGACAGCGGCGTGAGAACTGCACCGACCGTATGGAAATT‑ACCGCCATAGGTCAGCGCCTCAAAATAGGGCTGCCAGTCGAg                                                                      >  2:490302/1‑140 (MQ=255)                                                             gTGCCGGTAGCAGAACCAGAGACGCTGCGATCGCCCGCAATTAACGTAAAGGCCGGAACAGACAGCGGCGTGAGAACTGCACCGACCGTATGGAAATT‑ACCGCCATAGGTCAGCGCCTCAAAATAGGGCTGCCAATCGAg                                                                      >  1:538015/1‑140 (MQ=255)                                                               gCCGGTAGCAGAACCAGAGACGCTGCGATCGCCCGCAATTAACGTAAAGGCCGGAACAGACAGCGGCGTGAGAACTGCACCGACCGTATGGAAATT‑ACCGCCATAGGTCAGCGCCTCAAAATAGGGCTGCCAGTCGAGGc                                                                    <  2:420341/140‑1 (MQ=255)                                                                ccGGTAGCAGAACCAGAGACGCTGCGATCGCCCGCAATTAACGTAAAGGCCGGAACAGACAGCGGCGTGAGAACTGCACCGACCGTATGGAAATT‑ACCGCCATAGGTCAGCGCCTCAAAATAGGGCTGCCAGTCGAGGCt                                                                   <  1:641448/140‑1 (MQ=255)                                                                   gTAGCAGAACCAGAGACGCGGCGATCGCCCGCAATTAACGTAAAGGCCGGAACAGACAGCGGCGTGAGAACTGCACCGACCGTATGGAAATT‑ACCGCCATAGGTCAGCGCCTCAAAATAGGGCTGCCAGTCGAGGCTGAc                                                                <  2:80249/140‑1 (MQ=255)                                                                     aGCAGAACCAGAGACGCTGCGATCGCCCGCAATTAACGTAAAGGCCGGAACAGACAGCGGCGTGAGAACTGCACCGACCGTATGGAAATT‑ACCGCCATAGGTCAGCGCCTCAAAATAGGGCTGCCAGTCGAGGCTGACGt                                                              >  2:81147/1‑140 (MQ=255)                                                                        aGAACCAGAGACGCTGCGATCGCCCGCAATTAACGTAAAGGCCGGAACAGACAGCGGCGTGAGAACTGCACCGACCGTATGGAAATT‑ACCGCCATAGGTCAGCGCCTCAAAATAGGGCTGCCAGTCGAGGCTGACGTTGa                                                           <  2:372046/140‑1 (MQ=255)                                                                           aCCAGAGACGCTGCGATCGCCCGCAATTAACGTAAAGGCCGGAACAGACAGCGGCGTGAGAACTGCACCGACCGTATGGAAATT‑ACCGCCATAGGTCAGCGCCTCAAAATAGGGCTGCCAGTCGAGGCTGACGTTGACgg                                                        <  1:81147/140‑1 (MQ=255)                                                                                    gCTGCGATCGCCCGCAATTAACGTAAAGGCCGGAACAGACAGCGGCGTGAGAACTGCACCGACCGTATGGAAATT‑ACCGCCATAGGTCAGCGCCTCAAAATAGGGCTGCCAGTCGAGGCTGACGTTGa                                                           >  1:172156/1‑128 (MQ=255)                                                                                    gCTGCGATCGCCCGCAATTAACGTAAAGGCCGGAACAGACAGCGGCGTGAGAACTGCACCGACCGTATGGAAATT‑ACCGCCATAGGTCAGCGCCTCAAAATAGGGCTGCCAGTCGAGGCTGACGTTGa                                                           <  2:172156/128‑1 (MQ=255)                                                                                    gCTGCGATCGCCCGCAATTAACGTAAAGGCCGGAACAGACAGCGGCGTGAGAACTGCACCGACCGTATGGAAATT‑ACCGCCATAGGTCAGCGCCTCAAAATAGGGCTGCCAGTCGAGGCTGACGTTGACGGTGTTGATaa                                               <  1:818406/140‑1 (MQ=255)                                                                                     cTGCGATCGCCCGCAATTAACGTAAAGGCCGGAACAGACAGCGGCGTGAGAACTGCACCGACCGTATGGAAATT‑ACCGCCATAGGTCAGCGCCTCAAAATAGGGCTGCCAGTCGAGGCTGACGTTGACGGTGTTGATAAt                                              <  2:221697/140‑1 (MQ=255)                                                                                     cTGCGATCGCCCGCAATTAACGTAAAGGCCGGAACAGACAGCGGCGTGAGAACTGCACCGACCGTATGGAAATT‑ACCGCCATAGGTCAGCGCCTCAAAATAGGGCTGCCAGTCGAGGCTGACGTTGACGGTGTTGATAAt                                              <  1:218782/140‑1 (MQ=255)                                                                                       gCGATCGCCCGCAATTAACGTAAAGGCCGGAACAGACAGCGGCGTGAGAACTGCACCGACCGTATGGAAATT‑ACCGCCATAGGTCAGCGCCTCAAAATAGGGCTGCCAGTCGAGGCTGACGTTGACGGTGTTGATAATga                                            >  1:763853/1‑140 (MQ=255)                                                                                                  cAATTAACGTAAAGGCCGGAACAGACAGCGGCGTGAGAACTGCACCGACCGTATGGAAATT‑ACCGCCATAGGTCAGCGCCTCAAAATAGGGCTGCCAGTCGAGGCTGACGTTGACGGTGTTGATAATGAGATCAAACTGc                                 >  2:660481/1‑140 (MQ=255)                                                                                                             aaGGCCGGAACAGACAGCGGCGTGAGAACTGCACCGACCGTATGGAAATT‑ACCGCCATAGGTCAGCGCCTCAAAATAGGGCTGCCAGTCGAGGCTGACGTTGACGGTGTTGATAATGAGATCAAACTGCCCCGCCAGTGc                      >  1:838858/1‑140 (MQ=255)                                                                                                                 cggaaaCAGACGGGGGCGAAAAACCCCCCCCGCCCGTAGAAAAATC‑CCCGCCATAGGTCAGCGCCTAAAAATAGGGCTGCCAGTCGAGGCTGACGTTGACGGTGTTGATAATGAGATCAAACTGCCCCGCCAGTGCTTTc                  <  2:763853/136‑1 (MQ=255)                                                                                                                   ggAACAGACAGCGGCGTGAGAACTGCACCGACCGTATGGAAATT‑ACCGCCATAGGTCAGCGCCTCAAAATAGGGCTGCCAGTCGAGGCTGACGTTGACGGTGTTGATAATGAGATCAAACTGCCCCGCCAGTGCTTTCAg                >  1:931499/1‑140 (MQ=255)                                                                                                                       cagacagCGGCGTGAGAACTGCACCGACCGTATGGAAATT‑ACCGCCATAGGTCAGCGCCTCAAAATAGGGCTGCCAGTCGAGGCTGACGTTGACGGTGTTGATAATGAGATCAAACTGCCCCGCCAGTGCTTTCAGTGcc            >  2:68258/1‑140 (MQ=255)                                                                                                                          acagCGGCGTGAGAACTGCACCGACCGTATGGAAATT‑ACCGCCATAGGTCAGCGCCTCAAAATAGGGCTGCCAGTCGAGGCTGACGTTGACGGTGTTGATAATGAGATCAAACTGCCCCGCCAGTGCTTTCAGTGCCTGc         >  2:172171/1‑140 (MQ=255)                                                                                                                          acagCGGCGTGAGAACTGCACCGACCGTATGGAAATT‑ACCGCCATAGGTCAGCGCCTCAAAATAGGGCTGCCAGTCGAGGCTGACGTTGACGGTGTTGATAATGAGATCAAACTGCCCCGCCAGTGCTTTCAGTGCCTGc         >  1:70858/1‑140 (MQ=255)                                                                                                                                  aaaagaACCCCCCCCGCCGTTAGGAAATTCCCCGCCATAGGTCAGCGCCTAAAAATAGGGCTGCCAGTCGAGGCTGACGTTGAGGGTGTTGATAATGAGATCAAACTGCCCCGCCAGTGCTTTCAGTGCCTGCGGATcgc  <  2:125481/137‑1 (MQ=255)                                                                                                                                          |                                                                                                                                    GTGGTCGGCGCAACCTTGCTGCGGGCGGCAAAACGCATCAGCTTACGCAGCTCATAAGGCGTGCCGGTAGCAGAACCAGAGACGCTGCGATCGCCCGCAATTAACGTAAAGGCCGGAACAGACAGCGGCGTGAGAACCGCACCGACCGTATGGAAATT‑ACCGCCATAGGTCAGCGCCTCAAAATAGGGCTGCCAGTCGAGGCTGACGTTGACGGTGTTGATAATGAGATCAAACTGCCCCGCCAGTGCTTTCAGTGCCTGCGGATCGC  >  NC_000913/4495284‑4495551

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑