Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,504,834	A→G	I259M (ATA→ATG)	yjhB →	putative sialic acid transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,504,834	0	A	G	100.0%	223.3 / NA	61	I259M (ATA→ATG)	yjhB	putative sialic acid transporter
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (30/31);  total (30/31)

CTTTCAATCTCTATGATCGTTTTCCTCGTCTGTTTTTGTCTATTTGGTGCAAACTGGCCGATAAACGGACTACTTCCTTCCTACCTGGCAGATAATGGAGTTAATACAGTGGTCATTTCAACTCTGATGACAATAGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGTAAAAAAAGCCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTCTTTTTTTTATTTCTGT  >  NC_000913/4504700‑4504965                                                                                                                                       |                                                                                                                                    cTTTCAATCTCTATGATCGTTTTCCTCGTCTGTTTTTGTCTATTTGGTGCAAACTGGCCGATAAACGGACTACTTCCTTCCTACCTGGCAGATAATGGAGTTAATACAGTGGTTATTTCAACTCTGATGACAATGGCAgg                                                                                                                                <  1:239851/140‑1 (MQ=255) cTTTCAATCTCTATGATCGTTTTCCTCGTCTGTTTTTGTCTATTTGGTGCAAACTGGCCGATAAACGGACTACTTCCTTCCTACCTGGCAGATAATGGAGTTAATACAGTGGTCATTTCAACTCTGATGACAATGGCAgg                                                                                                                                <  1:166739/140‑1 (MQ=255)   ttCAATCTCTATGATCGTTTTCCTCGTCTGTTTTTGTCTATTTGGTGCAAACTGGCCGATAAACGGACTACTTCCTTCCTACCTGGCAGATAATGGAGTTAATACAGTGGTCATTTCAACTCTGATGACAATGGCAGGtt                                                                                                                              >  1:266454/1‑140 (MQ=255)   ttCAATCTCTATGATCGTTTTCCTCGTCTGTTTTTGTCTATTTGGTGCAAACTGGCCGATAAACGGACTACTTCCTTCCTACCTGGCAGATAATGGAGTTAATACAGTGGTCATTTCAACTCTGATGACAATGGCAGGtt                                                                                                                              >  2:737542/1‑140 (MQ=255)      aaTCTCTATGATCGTTTTCCTCGTCTGTTTTTGTCTATTTGGTGCAAACTGGCCGATAAACGGACTACTTCCTTCCTACCTGGCAGATAATGGAGTTAATACAGTGGTCATTTCAACTCTGATGACAATGGCAGGTTTAg                                                                                                                           <  2:341912/140‑1 (MQ=255)         ctctATGATCGTTTTCCTCGTCTGTTTTTGTCTATTTGGTGCAAACTGGCCGATAAACGGACTACTTCCTTCCTACCTGGCAGATAATGGAGTTAATACAGTGGTCATTTCAACTCTGATGACAATGGCAGGTTTAGGaa                                                                                                                        <  2:164166/140‑1 (MQ=255)         ctctATGATCGTTTTCCTCGTCTGTTTTTGTCTATTTGGTGCAAACTGGCCGATAAACGGACTACTTCCTTCCTACCTGGCAGATAATGGAGTTAATACAGTGGTCATTTCAACTCTGATGACAATGGCAGGTTTAGGaa                                                                                                                        <  2:744425/140‑1 (MQ=255)         ctctATGATCGTTTTCCTCGTCTGTTTTTGTCTATTTGGTGCAAACTGGCCGATAAACGGACTACTTCCTTCCTACCTGGCAGATAATGGAGTTAATACAGTGGTCATTTCAACTCTGATGACAATGGCAGGTTTAGGaa                                                                                                                        <  2:434498/140‑1 (MQ=255)           ctATGATCGTTTTCCTCGTCTGTTTTTGTCTATTTGGTGCAAACTGGCCGATAAACGGACTACTTCCTTCCTACCTGGCAGATAATGGAGTTAATACAGTGGTCATTTCAACTCTGATGACAATGGCAGGTTTAAGAaca                                                                                                                      >  2:406232/1‑140 (MQ=255)            tATGATCGTTTTCCTCGTCTGTTTTTGTCTATTTGGTGCAAACTGGCCGATAAACGGACTACTTCCTTCCTACCTGGCAGATAATGGAGTTAATACAGTGGTCATTTCAACTCTGATGACAATGGCAGGTTTAGGAacac                                                                                                                     >  1:497254/1‑140 (MQ=255)            tATGATCGTTTTCCTCGTCTGTTTTTGTCTATTTGGTGCAAACTGGCCGATAAACGGACTACTTCCTTCCTACCTGGCAGATAATGGAGTTAATACAGTGGTCATTTCAACTCTGATGACAATGGCAGGTTTAGGAacac                                                                                                                     >  2:752282/1‑140 (MQ=255)             aTGATCGTTTTCCTCGTCTGTTTTTGTCTATTTGGTGCAAACTGGCCGATAAACGGACTACTTCCTTCCTACCTGGCAGATAATGGAGTTAATACAGTGGTCATTTCAACTCTGATGACAATGGCAGGTTTAGGAACACt                                                                                                                    <  2:266454/140‑1 (MQ=255)                aTCGTTTTCCTCGTCTGTTTTTGTCTATTTGGTGCAAACTGGCCGATAAACGGACTACTTCCTTCCTACCTGGCAGATAATGGAGTTAATACAGTGGTCATTTCAACTCTGATGACAATGGCAGGTTTAGGAACACTGAc                                                                                                                 <  1:589189/140‑1 (MQ=255)                    ttttCCTCGTCTGTTTTTGTCTATTTGGTGCAAACTGGCCGATAAACGGACTACTTCCTTCCTACCTGGCAGATAATGGAGTTAATACAGTGGTCATTTCAACTCTGATGACAATGGCAGGTTTAGGAACACTGACAggg                                                                                                             >  2:220323/1‑139 (MQ=255)                    ttttCCTCGTCTGTTTTTGTCTATTTGGTGCAAACTGGCCGATAAACGGACTACTTCCTTCCTACCTGGCAGATAATGGAGTTAATACAGTGGTCATTTCAACTCTGATGACAATGGCAGGTTTAGGAACACTGACAGGt                                                                                                             >  1:503033/1‑140 (MQ=255)                      ttCCTCGTCTGTTTTTGTCTATTTGGTGCAAACTGGCCGATAAACGGACTACTTCCTTCCTACCTGGCAGATAATGGAGTTAATACAGTGGTCATTTCAACTCTGATGACAATGGCAGGTTTAg                                                                                                                           >  1:951347/1‑124 (MQ=255)                      ttCCTCGTCTGTTTTTGTCTATTTGGTGCAAACTGGCCGATAAACGGACTACTTCCTTCCTACCTGGCAGATAATGGAGTTAATACAGTGGTCATTTCAACTCTGATGACAATGGCAGGTTTAg                                                                                                                           <  2:951347/124‑1 (MQ=255)                        ccTCGTCTGTTTTTGTCTATTTGGTGCAAACTGGCCGATAAACGGACTACTTCCTTCCTACCTGGCAGATAATGGAGTTAATACAGTGGTCATTTCAACTCTGATGACAATGGCAGGTTTAGGAACACTGACAGGTACaa                                                                                                         <  2:630648/140‑1 (MQ=255)                        ccTCGTCTGTTTTTGTCTATTTGGTGCAAACTGGCCGATAAACGGACTACTTCCTTCCTACCTGGCAGATAATGGAGTTAATACAGTGGTCATTTCAACTCTGATGACAATGGCAGGTTTAGGAACACTGACAGGTACaa                                                                                                         <  2:774080/140‑1 (MQ=255)                            gTCTGTTTTTGTCTATTTGGTGCAAACTGGCCGATAAACGGACTACTTCCTTCCTACCTGGCAGATAATGGAGTTAATACAGTGGTCATTTCAACTCTGATGACAATGGCAGGTTTAGGAACACTGACAGGTACAATAtt                                                                                                     >  2:478860/1‑140 (MQ=255)                               tGTTTTTGTCTATTTGGTGCAAACTGGCCGATAAACGGACTACTTCCTTCCTACCTGGCAGATAATGGAGTTAATACAGTGGTCATTTCAACTCTGATGACAATGGCAGGTTTAGGAACACTGACAGGTACAATAttttt                                                                                                  >  1:503013/1‑140 (MQ=255)                                       tCTATTTGGTGCAAACTGGCCGATAAACGGACTACTTCCTTCCTACCTGGCAGATAATGGAGTTAATACAGTGGTCATTTCAACTCTGATGACAATGGCAGGTTTAAGAAAACTGACAGGTAAAATATTTTTTGGttttt                                                                                          >  2:361994/1‑139 (MQ=255)                                        cTATTTGGTGCAAACTGGCCGATAAACGGACTACTTCCTTCCTACCTGGCAGATAATGGAGTTAATACAGTGGTCATTTCAACTCTGATGACAATGGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTttgt                                                                                         <  1:649723/140‑1 (MQ=255)                                         tATTTGGTGCAAACTGGCCGATAAACGGACTACTTCCTTCCTACCTGGCAGATAATGGAGTTAATACAGTGGTCATTTCAACTCTGATGACAATGGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTttgtt                                                                                        <  1:737542/140‑1 (MQ=255)                                         tATTTGGTGCAAACTGGCCGATAAACGGACTACTTCCTTCCTACCTGGCAGATAATGGAGTTAATACAGTGGTCATTTCAACTCTGATGACAATGGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTttgtt                                                                                        <  2:843284/140‑1 (MQ=255)                                               gTGCAAACTGGCCGATAAACGGACTACTTCCTTCCTACCTGGCAGATAATGGAGTTAATACAGTGGTCATTTCAACTCTGATGACAATGGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGAt                                                                                  <  1:752282/140‑1 (MQ=255)                                                    aaCTGGCCGATAAACGGCCTACTTCCTTCCTACCTGGCAGATAATGGAGTTAATACAGTGGTCATTTCAACTCTGATGACAATGGCAGGTTTAGGAACACTGACAGGTACAATATGCTTTGGTTTTGTTGGTGATAAGAt                                                                             <  1:466356/140‑1 (MQ=255)                                                    aaCTGGCCGATAAACGGACTACTTCCTTCCTACCTGGCAGATAATGGAGTTAATACAGTGGTCATTTCAACTCTGATGACAATGGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGAt                                                                             <  2:412055/140‑1 (MQ=255)                                                         gtcGATAAACGGACTACTTCCTTCCTACCTGGCAGATAATGGAGTTAATACAGTGGTCATTTCAACTCTGATGACAATGGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGgtg                                                                        >  2:560789/3‑140 (MQ=255)                                                         gCCGATAAACGGACTACTTCCTTCCTACCTGGCAGATAATGGAGTTAATACAGTGGTCATTTCAACTCTGATGACAATGGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTttgttg                                                                                       >  1:89213/1‑125 (MQ=255)                                                         gCCGATAAACGGACTACTTCCTTCCTACCTGGCAGATAATGGAGTTAATACAGTGGTCATTTCAACTCTGATGACAATGGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTttgttg                                                                                       <  2:89213/125‑1 (MQ=255)                                                         gCCGATAAACGGACTACTTCCTTCCTACCTGGCAGATAATGGAGTTAATACAGTGGTCATTTCAACTCTGATGACAATGGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTttgttg                                                                                       <  2:369111/125‑1 (MQ=255)                                                         gCCGATAAACGGACTACTTCCTTCCTACCTGGCAGATAATGGAGTTAATACAGTGGTCATTTCAACTCTGATGACAATGGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTttgttg                                                                                       >  1:369111/1‑125 (MQ=255)                                                         gCCGATAAACGGACTACTTCCTTCCTACCTGGCAGATAATGGAGTTAATACAGTGGTCATTTCAACTCTGATGACAATGGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGgtg                                                                        <  1:659812/140‑1 (MQ=255)                                                                aaCGGACTACTTCCTTCCTACCTGGCAGATAATGGAGTTAATACAGTGGTCATTTCAACTCTGATGACAATGGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGTaaaaaa                                                                 >  2:721995/1‑140 (MQ=255)                                                                aaCGGACTACTTCCTTCCTACCTGGCAGATAATGGAGTTAATACAGTGGTCATTTCAACTCTGATGACAATGGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGTaaaaaa                                                                 <  2:497254/140‑1 (MQ=255)                                                                 aCGGACTACTTCCTTCCTACCTGGCAGATAATGGAGTTAATACAGTGGTCATTTCAACTCTGATGACAATGGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGCTTTTGTTGGTGATAAGATTGGTGTaaaaaaa                                                                <  2:689956/140‑1 (MQ=255)                                                                           tccttcctACCTGGCAGATAATGGAGTTAATACAGTGGTCATTTCAACTCTGATGACAATGGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGTAAAAAAAGCCTTTGTAg                                                      <  1:478860/140‑1 (MQ=255)                                                                            ccttcctACCTGGCAGATAATGGAGTTAATACAGTGGTCATTTCAACTCTGATGACAATGGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGTAAAAAAGGCCTTTGTAGt                                                     <  2:606673/140‑1 (MQ=255)                                                                            ccttcctACCTGGCAGATAATGGAGTTAATACAGTGGTCATTTCAACTCTGATGACAATGGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGTAAAAAAAGCCTTTGTAGt                                                     <  2:559504/140‑1 (MQ=255)                                                                             cttcctACCTGGCAGATAATGGAGTTAATACAGTGGTCATTTCAACTCTGATGACAATGGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGTAAAAAAAGCCTTTGTAGTc                                                    <  1:721995/140‑1 (MQ=255)                                                                               tcctACCTGGCAGATAATGGAGTTAATACAGTGGTCATTTCAACTCTGATGACAATGGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGTAAAAAAAGCCTTTGTAGt                                                     <  1:790774/137‑1 (MQ=255)                                                                               tcctACCTGGCAGATAATGGAGTTAATACAGTGGTCATTTCAACTCTGATGACAATGGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGTAAAAAAAGCCTTTGTAGt                                                     >  2:790774/1‑137 (MQ=255)                                                                               tcctACCTGGCAGATAATGGAGTTAATACAGTGGTCATTTCAACTCTGATGACAATGGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGTAAAAAAAGCCTTTGTAGTCgg                                                  >  1:787544/1‑140 (MQ=255)                                                                                   aCCTGGCAGATAATGGAGTTAATACAGTGGTCATTTCAACTCTGATGACAATGGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGTAAAAAAAGCCTTTGTAGt                                                     <  2:133808/133‑1 (MQ=255)                                                                                   aCCTGGCAGATAATGGAGTTAATACAGTGGTCATTTCAACTCTGATGACAATGGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGTAAAAAAAGCCTTTGTAGt                                                     >  1:133808/1‑133 (MQ=255)                                                                                   aCCTGGCAGATAATGGAGTTAATACAGTGGTCATTTCAACTCTGATGACAATGGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGTAAAAAAAGCCTTTGTAGTCGGTCta                                              >  1:748111/1‑140 (MQ=255)                                                                                    ccTGGCAGATAATGGAGTTAATACAGTGGTCATTTCAACTCTGATGACAATGGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGTAAAAAAAGCCTTTGTAGTCGGTCtaa                                             >  1:251760/1‑140 (MQ=255)                                                                                            aTAATGGAGTTAATACAGTGGTCATTTCAACTCTGATGACAATGGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGTAAAAAAAGCCTTTGTAGTCGGTCTAATAACTTCa                                     <  1:560789/140‑1 (MQ=255)                                                                                            aTAATGGAGTTAATACAGTGGTCATTTCAACTCTGATGACAATGGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGTAAAAAAAGCCTTTGTAGTCGGTCTAATAACTTCa                                     >  1:508834/1‑140 (MQ=255)                                                                                              aaTGGAGTTAATACAGTGGTCATTTCAACTCTGATGACAATGGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGTAAAAAAAGCCTTTGTAGTCGGTCTAATAACTTCatt                                   >  2:631597/1‑140 (MQ=255)                                                                                                              tGGTCATTTCAACTCTGATGACAATGGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGTAAAAAAAGCCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCct                   >  1:408442/1‑140 (MQ=255)                                                                                                               ggTCATTTCAACTCTGATGACAATGGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGTAAAAAAAGCCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCt                          <  2:40961/132‑1 (MQ=255)                                                                                                               ggTCATTTCAACTCTGATGACAATGGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGTAAAAAAAGCCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCt                          >  1:40961/1‑132 (MQ=255)                                                                                                               ggTCATTTCAACTCTGATGACAATGGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGTAAAAAAAGCCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCctc                  >  1:778102/1‑140 (MQ=255)                                                                                                                          ctctGATGACAATGGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGTAAAAAAAGCCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCctct                 >  1:323436/1‑130 (MQ=255)                                                                                                                          ctctGATGACAATGGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGTAAAAAAAGCCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCctct                 <  2:323436/130‑1 (MQ=255)                                                                                                                          ctctGATGACAATGGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGTAAAAAAAGCCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTCTTTTTTTTAtt       >  2:8408/1‑140 (MQ=255)                                                                                                                               atgaCAATGGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGTAAAAAAAGCCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTCTTTTTTTTATTTCtgt  >  1:528883/1‑140 (MQ=255)                                                                                                                               atgaCAATGGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGTAAAAAAAGCCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTCTTTTTTTTATTTCtgt  >  2:375603/1‑140 (MQ=255)                                                                                                                               atgaCAATGGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGTAAAAAAAGCCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTCTTTTTTTTATTTCtgt  >  2:496632/1‑140 (MQ=255)                                                                                                                                       |                                                                                                                                    CTTTCAATCTCTATGATCGTTTTCCTCGTCTGTTTTTGTCTATTTGGTGCAAACTGGCCGATAAACGGACTACTTCCTTCCTACCTGGCAGATAATGGAGTTAATACAGTGGTCATTTCAACTCTGATGACAATAGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGTAAAAAAAGCCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTCTTTTTTTTATTTCTGT  >  NC_000913/4504700‑4504965

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑