Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	724,394	A→G	R7R (CGT→CGC)	kdpD ←	sensory histidine kinase KdpD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	724,394	0	A	G	100.0%	94.9 / NA	27	R7R (CGT→CGC)	kdpD	sensory histidine kinase KdpD
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (13/14);  total (13/14)

AGCCCTTGCGCCCGCAGTCGCTGGGCTTCTGCCAGCATCGCCCAGGTCTTCCCGACGCCTGCACAGGCACCGAAGAAAACTTTCAGCTTCCCCCGATGCGGCGCGGCAGTTTGTTCCAGCAGACGATCGGGGTCGGGACGTAAGGGTTCGTTATTCATCAAGTTTATCCAGCGCCAGATTGAGTTCAACAATGTTGACAACCGGCTGGCCGATATATTTCACCAGCGGTTGTTGGCTGTATTTTGCGATCAGTTGCGTGAGCT  >  NC_000913/724257‑724519                                                                                                                                          |                                                                                                                              aGCCCTTGCGCCCGCAGTCGCTGGGCTTCTGCCAGCATCGCCCAGGTCTTCCCGACGCCTGCACAGGCACCGAAGAAAACTTTCAGCTTCCCCCGATGCGGCGCGGCAGTTTGTTCCAGCAGACGATCGGGGTCGGGGCg                                                                                                                             >  1:826275/1‑140 (MQ=255)   cccTTGCGCCCGCAGTCGCTGGGCTTCTGCCAGCATCGCCCAGGTCTTCCCGACGCCTGCACAGGCACCGAAGAAAACTTTCAGCTTCCCCCGATGCGGCGCGGCAGTTTGTTCCAGCAGACGATCGGGGTCGGGGCGTa                                                                                                                           >  1:437433/1‑140 (MQ=255)    ccTTGCGCCCGCAGTCGCTGGGCTTCTGCCAGCATCGCCCAGGTCTTCCCGACGCCTGCACAGGCACCGAAGAAAACTTTCAGCTTCCCCCGATGCGGCGCGGCAGTTTGTTCCAGCAGACGATCGGGGTCGGGGCGTaa                                                                                                                          <  2:346137/140‑1 (MQ=255)      ttGCGCCCGCAGTCGCTGGGCTTCTGCCAGCATCGCCCAGGTCTTCCCGACGCCTGCACAGGCACCGAAGAAAACTTTCAGCTTCCCCCGATGCGGCGCGGCAGTTTGTTCCAGCAGACGATCGGGGTCGGGGCGTAAgg                                                                                                                        <  1:384916/140‑1 (MQ=255)       tGCGCCCGCAGTCGCTGGGCTTCTGCCAGCATCGCCCAGGTCTTCCCGACGCCTGCACAGGCACCGAAGAAGACTTTCAGCTTCCCCCGATGCGGCGCGGCAGTTTGTTCCAGCAGACGATCGGGGTCGGGGCGTAAggg                                                                                                                       >  2:423362/1‑140 (MQ=255)        gcgcCCGCAGTCGCTGGGCTTCTGCCAGCATCGCCCAGGTCTTCCCGACGCCTGCACAGGCACCGAAGAAAACTTTCAGCTTCCCCCGATGCGGCGCGGCAGTTTGTTCCAGCAGACGATCGGGGTCGGGGCGTAAGGGt                                                                                                                      >  1:359323/1‑140 (MQ=255)        gcgcCCGCAGTCGCTGGGCTTCTGCCAGCATCGCCCAGGTCTTCCCGACGCCTGCACAGGCACCGAAGAAAACTTTCAGCTTCCCCCGATGCGGCGCGGCAGTTTGTTCCAGCAGACGATCGGGGTCGGGGCGTAAGGGt                                                                                                                      >  1:305707/1‑140 (MQ=255)            ccGCAGTCGCTGGGCTTCTGCCAGCATCGCCCAGGTCTTCCCGACGCCTGCACAGGCACCGAAGAAAACTTTCAGCTTCCCCCGATGCGGCGCGGCAGTTTGTTCCAGCAGACGATCGGGGTCGGGGCGTAAGGGTTCGt                                                                                                                  <  2:437433/140‑1 (MQ=255)                   cGCTGGGCTTCTGCCAGCATCGCCCAGGTCTTCCCGACGCCTGCACAGGCACCGAAGAAAACTTTCAGCTTCCCCCGATGCGGCGCGGCAGTTTGTTCCAGCAGACGATCGGGGTCGGGGCGTAAGGGTTCGTTATtcat                                                                                                           <  2:305707/140‑1 (MQ=255)                   cGCTGGGCTTCTGCCAGCATCGCCCAGGTCTTCCCGACGCCTGCACAGGCACCGAAGAAAACTTTCAGCTTCCCCCGATGCGGCGCGGCAGTTTGTTCCAGCAGACGATCGGGGTCGGGGCGTAAGGGTTCGTTATtcat                                                                                                           <  2:321122/140‑1 (MQ=255)                               gCCAGCATCGCCCAGGTCTTCCCGACGCCTGCACAGGCACCGAAGAAAACTTTCAGCTTCCCCCGATGCGGCGCGGCAGTTTGTTCCAGCAGACGATCGGGGTCGGGGCGTAAGGGTTCGTTATTCATCAAGTTTATCCa                                                                                               <  1:369565/140‑1 (MQ=255)                               gCCAGCATCGCCCAGGTCTTCCCGACGCCTGCACAGGCACCGAAGAAAACTTTCAGCTTCCCCCGATGCGGCGCGGCAGTTTGTTCCAGCAGACGATCGGGGTCGGGGCGTAAGGGTTCGTTATTCATCAAGTTTATCCa                                                                                               <  2:359323/140‑1 (MQ=255)                                    cATCGCCCAGGTCTTCCCGACGCCTGCACAGGCACCGAAGAAGACTTTCAGCTTCCCCCGATGCGGCGCGGCAGTTTGTTCCAGCAGACGATCGGGGTCGGGGCGTAAGGGTTCGTTATTCATCAAGTTTATCCAGCGcc                                                                                          <  1:423362/140‑1 (MQ=255)                                         cccAGGTCTTCCCGACGCCTGCACAGGCACCGAAGAAAACTTTCAGCTTCCCCCGATGCGGCGCGGCAGTTTGTTCCAGCAGACGATCGGGGTCGGGGCGTAAGGGTTCGTTATTCATCAAGTTTATCCAGCGCCAGAtt                                                                                     >  1:403591/1‑140 (MQ=255)                                                cTTCCCGACGCCTGCACAGGCACCGAAGAAAACTTTCAGCTTCCCCCGATGCGGCGCGGCAGTTTGTTCCAGCAGACGATCGGGGTCGGGGCGTAAGGGTTCGTTATTCATCAAGTTTATCCAGCGCCAGATTGAGTTca                                                                              <  2:403591/140‑1 (MQ=255)                                                 ttCCCGACGCCTGCACAGGCACCGAAGAAAACTTTCAGCTTCCCCCGATGCGGCGCGGCAGTTTGTTCCAGCAGACGATCGGGGTCGGGGCGTAAGGGTTCGTTATTCATCAAGTTTATCCAGCGCCAGATTGAGTTcaa                                                                             <  2:843333/140‑1 (MQ=255)                                                   cccGACGCCTGCACAGGCACCGAAGAAAACTTTCAGCTTCCCCCGATGCGGCGCGGCAGTTTGTTCCAGCAGACGATCGGGGTCGGGGCGTAAGGGTTCGTTATTCATCAAGTTTATCCAGCGCCAGATTGAGTTcaaca                                                                           >  2:163295/1‑140 (MQ=255)                                                   cccGACGCCTGCACAGGCACCGAAGAAAACTTTCAGCTTCCCCCGATGCGGCGCGGCAGTTTGTTCCAGCAGACGATCGGGGTCGGGGCGTAAGGGTTCGTTATTCATCAAGTTTATCCAGCGCCAGATTGAGTTcaaca                                                                           >  2:420157/1‑140 (MQ=255)                                                   cccGACGCCTGCACAGGCACCGAAGAAAACTTTCAGCTTCCCCCGATGCGGCGCGGCAGTTTGTTCCAGCAGACGATCGGGGTCGGGGCGTAAGGGTTCGTTATTCATCAAGTTTATCCAGCGCCAGATTGAGTTcaaca                                                                           >  2:271278/1‑140 (MQ=255)                                                        cGCCTGCACAGGCACCGAAGAAAACTTTCAGCTTCCCCCGATGCGGCGCGGCAGTTTGTTCCAGCAGACGATCGGGGTCGGGGCGTAAGGGTTCGTTATTCATCAAGTTTATCCAGCGCCAGATTGAGTTCAACAATGtt                                                                      >  2:668506/1‑140 (MQ=255)                                                                   gCACCGAAGAAAACTTTCAGCTTCCCCCGATGCGGCGCGGCAGTTTGTTCCAGCAGACGATCGGGGTCGGGGCGTAAGGGTTCGTTATTCATCAAGTTTATCCAGCGCCAGATTGAGTTCAACAATGTTGACAACCGGCt                                                           >  1:815185/1‑140 (MQ=255)                                                                   gCACCGAAGAAAACTTTCAGCTTCCCCCGATGCGGCGCGGCAGTTTGTTCCAGCAGACGATCGGGGTCGGGGCGTAAGGGTTCGTTATTCATCAAGTTTATCCAGCGCCAGATTGAGTTCAACAATGTTGACAACCGGCt                                                           <  1:668506/140‑1 (MQ=255)                                                                     aCCGAAGAAAACTTTCAGCTTCCCCCGATGCGGCGCGGCAGTTTGTTCCAGCAGACGATCGGGGTCGGGGCGTAAGGGTTCGTTATTCATCAAGTTTATCCAGCGCCAGATTGAGTTCAACAATGTTGACAACCGGCTgg                                                         >  2:543459/1‑140 (MQ=255)                                                                           gaaAACTTTCAGCTTCCCCCGATGCGGCGCGGCAGTTTGTTCCAGCAGACGATCGGGGTCGGGGCGTAAGGGTTCGTTATTCATCAAGTTTATCCAGCGCCAGATTGAGTTCAACAATGTTGACAACCGGCTGGCCGata                                                   <  1:420157/140‑1 (MQ=255)                                                                                                  gcggcgcggcAGTTTGTTCCAGCAGACGATCGGGGTCGGGGCGTAAGGGTTCGTTATTCATCAAGTTTATCCAGCGCCAGATTGAGTTCAACAATGTTGACAACCGGCTGGCCGATATATTTCACCAGCGGTTGTTGGCt                            <  1:546972/140‑1 (MQ=255)                                                                                                            aGTTTGTTCCAGCAGACGATCGGGGTCGGGGCGTAAGGGTTCGTTATTCATCAAGTTTATCCAGCGCCAGATTGAGTTCAACAATGTTGACAACCGGCTGGCCGATATATTTCACCAGCGGTTGTTGGCTGTATTTTGCg                  <  2:815185/140‑1 (MQ=255)                                                                                                                            cGATCGGGGTCGGGGCGTAAGGGTTCGTTATTCATCAAGTTTATCCAGCGCCAGATTGAGTTCAACAATGTTGACAACCGGCTGGCCGATATATTTCACCAGCGGTTGTTGGCTGTATTTTGCGATCAGTTGCGTGAGCt  >  1:334863/1‑140 (MQ=255)                                                                                                                                          |                                                                                                                              AGCCCTTGCGCCCGCAGTCGCTGGGCTTCTGCCAGCATCGCCCAGGTCTTCCCGACGCCTGCACAGGCACCGAAGAAAACTTTCAGCTTCCCCCGATGCGGCGCGGCAGTTTGTTCCAGCAGACGATCGGGGTCGGGACGTAAGGGTTCGTTATTCATCAAGTTTATCCAGCGCCAGATTGAGTTCAACAATGTTGACAACCGGCTGGCCGATATATTTCACCAGCGGTTGTTGGCTGTATTTTGCGATCAGTTGCGTGAGCT  >  NC_000913/724257‑724519

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑