Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	800,297	A→G	G93G (GGT→GGC)	ybhD ←	putative DNA‑binding transcriptional regulator YbhD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	800,297	0	A	G	100.0%	146.1 / NA	41	G93G (GGT→GGC)	ybhD	putative DNA‑binding transcriptional regulator YbhD
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (21/20);  total (21/20)

GCACCGATTCCATGCAATTATTCGTGCCTTGTTCCAGAATCCGCACTTTAATATTGGGATACAGCTCATTAAATTTACCGATTGCCAGCGGTAAAAAATAAAATACTGCGGTCGGAATACACGCTAACGTCACCATACCACGATGATAAGCATTCATATCACGAATATTAAAAAGCGTTTCATCGAATTTCTTTATTAATTCCCGCGCCTCCGGGAGCAACCTTTTTCCTGCTTTTGTTAACGTAACCTTGCGAGTTGTACGCTCA  >  NC_000913/800161‑800426                                                                                                                                         |                                                                                                                                  gCACCGATTCCATGCAATTATTCGTGCCTTGTTCCAGAATCCGCACTTTAATATTGGGATACAGCTCATTAAATTTACCGATTGCCAGCGGTAAAAAATAAAATACTGCGGTCGGAATACACGCTAACGTCACCATGCCa                                                                                                                                >  1:930293/1‑140 (MQ=255)  cACCGATTCCATGCAATTATTCGTGCCTTGTTCCAGAATCCGCACTTTAATATTGGGATACAGCTCATTAAATTTACCGATTGCCAGCGGTAAAAAATAAAATACTGCGGTCGGAATACACGCTAACGTCACCATGCCAc                                                                                                                               >  2:333200/1‑140 (MQ=255)  cACCGATTCCATGCAATTATTCGTGCCTTGTTCCAGAATCCGCACTTTAATATTGGGATACAGCTCATTAAATTTACCGATTGCCAGCGGTAAAAAATAAAATACTGCGGTCGGAATACACGCTAACGTCACCATGCCAc                                                                                                                               >  2:365157/1‑140 (MQ=255)   aCCGATTCCATGCAATTATTCGTGCCTTGTTCCAGAATCCGCACTTTAATATTGGGATACAGCTCATTAAATTTACCGATTGCCAGCGGTAAAAAATAAAATACTGCGGTCGGAATACACGCTAACGTCACCATGCCACg                                                                                                                              <  1:333200/140‑1 (MQ=255)       aTTCCATGCAATTATTCGTGCCTTGTTCCAGAATCCGCACTTTAATATTGGGATACAGCTCATTAAATTTACCGATTGCCAGCGGTAAAAAATAAAATACTGCGGTCGGAATACACGCTAACGTCACCATGCCACgatga                                                                                                                          >  2:342189/1‑140 (MQ=255)           cATGCAATTATTCGTGCCTTGTTCCAGAATCCGCACTTTAATATTGGGATACAGCTCATTAAATTTACCGATTGCCAGCGGTAAAAAATAAAATACTGCGGTCGGAATACACGCTAACGTCACCATGCCACGATGATAAg                                                                                                                      <  1:66885/140‑1 (MQ=255)             tGCAATTATTCGTGCCTTGTTCCAGAATCCGCACTTTAATATTGGGATACAGCTCATTAAATTTACCGATTGCCAGCGGTAAAAAATAAAATACTGCGGTCGGAATACACGCTAACGTCACCATGCCACGATGATAAGCa                                                                                                                    <  2:105238/140‑1 (MQ=255)                         tGCCTTGTTCCAGAATCCGCACTTTAATATTGGGATACAGCTCATTAAATTTACCGATTGCCAGAGGTAAAAAATAAAATACTGCGGTCGGAATACACGCTAACGTCACCATGCCACGATGATAAGCATTCATATCACGa                                                                                                        >  2:261049/1‑140 (MQ=255)                          gCCTTGTTCCAGAATCCGCACTTTAATATTGGGATACAGCTCATTAAATTTACCGATTGCCAGCGGTAAAAAATAAAATACTGCGGTCGGAATACACGCTAACGTCACCATGCCACGATGATAAGCATTCATATCACGaa                                                                                                       <  2:779828/140‑1 (MQ=255)                                         ccGCACTTTAATATTGGGATACAGCTCATTAAATTTACCGATTGCCAGCGGTAAAAAATAAAATACTGCGGTCGGAATACACGCTAACGTCACCATGCCACGATGATAAGCATTCATATCACGAATATTAAAAAGCGttt                                                                                        <  1:75249/140‑1 (MQ=255)                                          cGCACTTTAATATTGGGATACAGCTCATTAAATTTACCGATTGCCAGCGGTAAAAAATAAAATACTGCGGTCGGAATACACGCTAACGTCACCATGCCACGATGATAAGCATTCATATCACGAATATTAAAAAGCGTTTc                                                                                       >  2:368620/1‑140 (MQ=255)                                          cGCACTTTAATATTGGGATACAGCTCATTAAATTTACCGATTGCCAGCGGTAAAAAATAAAATACTGCGGTCGGAATACACGCTAACGTCACCATGCCACGATGATAAGCATTCATATCACGAATATTAAAAAGCGTTTc                                                                                       >  2:205661/1‑140 (MQ=255)                                          cGCACTTTAATATTGGGATACAGCTCATTAAATTTACCGATTGCCAGCGGTAAAAAATAAAATACTGCGGTCGGAATACACGCTAACGTCACCATGCCACGATGATAAGCATTCATATCACGAATATTAAAAAGCGTTTc                                                                                       >  2:816935/1‑140 (MQ=255)                                              cTTTAATATTGGGATACAGCTCATTAAATTTACCGATTGCCAGCGGTAAAAAATAAAATACTGCGGTCGGAATACACGCTAACGTCACCATGCCACGATGATAAGCATTCATATCACGAATATTAAAAAGCGTTTCATCg                                                                                   <  1:342189/140‑1 (MQ=255)                                                ttAATATTGGGATACAGCTCATTAAATTTACCGATTGCCAGCGGTAAAAAATAAAATACTGCGCTCGGAATACACGCTAACGTCACCATGCCACGATGATAAGCATTCATATCACGAATATTAAAAAGCGTTTCATCGaa                                                                                 <  1:806693/140‑1 (MQ=255)                                                         ggATACAGCTCATTAAATTTACCGATTGCCAGCGGTAAAAAATAAAATACTGCGGTCGGAATACACGCTAACGTCACCATGCCACGATGATAAGCATTCATATCACGAATATTAAAAAGCGTTTCATCGAATTTCTttat                                                                        >  2:511732/1‑140 (MQ=255)                                                         ggATACAGCTCATTAAATTTACCGATTGCCAGCGGTAAAAAATAAAATACTGCGGTCGGAATACACGCTAACGTCACCATGCCACGATGATAAGCATTCATATCACGAATATTAAAAAGCGTTTCATCGAATTTCTttat                                                                        >  1:502205/1‑140 (MQ=255)                                                                gCTCATTAAATTTACCGATTGCCAGCGGTAAAAAATAAAATACTGCGGTCGGAATACACGCTAACGTCACCATGCCACGATGATAAGCATTCATATCACGAATATTAAAAAGCGTTTCATCGAATTTCTTTATTAATTcc                                                                 >  2:576171/1‑140 (MQ=255)                                                                 cTCATTAAATTTACCGATTGCCAGCGGTAAAAAATAAAATACTGCGGTCGGAATACACGCTAACGTCACCATGCCACGATGATAAGCATTCATATCACGAATATTAAAAAGCGTTTCATCGAATTTCTTTATTAATTccc                                                                >  2:846681/1‑140 (MQ=255)                                                                 cTCATTAAATTTACCGATTGCCAGCGGTAAAAAATAAAATACTGCGGTCGGAATACACGCTAACGTCACCATGCCACGATGATAAGCATTCATATCACGAATATTAAAAAGCGTTTCATCGAATTTCTTTATTAATTccc                                                                >  1:458860/1‑140 (MQ=255)                                                                  tCATTAAATTTACCGATTGCCAGCGGTAAAAAATAAAATACTGCGGTCGGAATACACGCTAACGTCACCATGCCACGATGATAAGCATTCATATCACGAATATTAAAAAGCGTTTCATCGAATTTCTTTATTAATTCCcg                                                               <  1:365157/140‑1 (MQ=255)                                                                  tCATTAAATTTACCGATTGCCAGCGGTAAAAAATAAAATACTGCGGTCGGAATACACGCTAACGTCACCATGCCACGATGATAAGCATTCATATCACGAATATTAAAAAGCGTTTCATCGAATTTCTTTATTAATTCCcg                                                               <  2:502205/140‑1 (MQ=255)                                                                   cATTAAATTTACCGATTGCCAGCGGTAAAAAATAAAATACTGCGGTCGGAATACATGCTAACGTCACCATGCCACGATGATAAGCATTCATATCACGAATATTAAAAAGCGTTTCATCGAATTTCTTTATTAATTCCcgc                                                              >  1:264678/1‑140 (MQ=255)                                                                    aTTAAATTTACCGATTGCCAGCGGTAAAAAATAAAATACTGCGGTCGGAATACACGCTAACGTCACCATGCCACGATGATAAGCATTCATATCACGAATATTAAAAAGCGTTTCATCGAATTTCTTTATTAATTCCcgcg                                                             <  1:205661/140‑1 (MQ=255)                                                                      tAAATTTACCGATTGCCAGCGGTAAAAAATAAAATACTGCGGTCGGAATACACGCTAACGTCACCATGCCACGATGATAAGCATTCATATCACGAATATTAAAAAGCGTTTCATCGAATTTCTTTATTAATTCCCGCGcc                                                           >  2:819828/1‑140 (MQ=255)                                                                        aaTTTACCGATTGCCAGCGGTAAAAAATAAAATACTGCGGTCGGAATACACGCTAACGTCACCATGCCACGATGATAAGCATTCATATCACGAATATTAAAAAGCGTTTCATCGAATTTCTTTATTAATTCCCGCGCCTc                                                         <  1:846681/140‑1 (MQ=255)                                                                              ccGATTGCCAGCGGTAAAAAATAAAATACTGCGGTCGGAATACACGCTAACGTCACCATGCCACGATGATAAGCATTCATATCACGAATATTAAAAAGCGTTTCATCGAATTTCTTTATTAATTCCCGCGCCTCCGGGAg                                                   >  1:410946/1‑140 (MQ=255)                                                                                         cGGTAAAAAATAAAATACTGCGGTCGGAATACACGCTAACGTCACCATGCCACGATGATAAGCATTCATATCACGAATATTAAAAAGCGTTTCATCGAATTTCTTTATTAATTCCCGCGCCTCCGGGAGCAACCTTTTTc                                        <  2:585306/140‑1 (MQ=255)                                                                                         aggTAAAAAATAAAATACTGCGGTCGGAATACACGCTAACGTCACCATGCCACGATGATAAGCATTCATATCACGAATATTAAAAAGCGTTTCATCGAATTTCTTTATTAATTCCCGCGCCTCCGGGAGCAACCTTTTTc                                        <  1:261049/139‑1 (MQ=255)                                                                                                 aataaaataCTGCGGTCGGAATACACGCTAACGTCACCATGCCACGATGATAAGCATTCATATCACGAATATTAAAAAGCGTTTCATCGAATTTCTTTATTAATTCCCGCGCCTCCGGGAGCAACCTTTTTCCTGCtttt                                <  1:368620/140‑1 (MQ=255)                                                                                                 aataaaataCTGCGGTCGGAATACACGCTAACGTCACCATGCCACGATGATAAGCATTCATATCACGAATATTAAAAAGCGTTTCATCGAATTTCTTTATTAATTCCCGCGCCTCCGGGAGCAACC‑TTTTCCTGCTTTTg                               <  2:458860/140‑1 (MQ=255)                                                                                                  ataaaataCTGCGGTCGGAATACACGCTAACGTCACCATGCCACGATGATAAGCATTCATATCACGAATATTAAAAAGCGTTTCATCGAATTTCTTTATTAATTCCCGCGCCTCCGGGAGCAACCTTTTTCCTGCTTTTg                               <  1:341633/140‑1 (MQ=255)                                                                                                       ataCTGCGGTCGGAATACACGCTAACGTCACCATGCCACGATGATAAGCATTCATATCACGAATATTAAAAAGCGTTTCATCGAATTTCTTTATTAATTCCCGCGCCTCCGGGAGCAACCTTTTTCCTGCTTTTGTTAAc                          <  1:576171/140‑1 (MQ=255)                                                                                                           tGCGGTCGGAATACACGCTAACGTCACCATGCCACGATGATAAGCATTCATATCACGAATATTAAAAAGCGTTTCATCGAATTTCTTTATTAATTCCCGCGCCTCCGGGAGCAACCTTTTTCCTGCTTTTGTTAACGTaa                      >  2:68387/1‑140 (MQ=255)                                                                                                                       acacGCTAACGTCACCATGCCACGATGATAAGCATTCATATCACGAATATTAAAAAGCGTTTCATCGAATTTCTTTATTAATTCCCGCGCCTCCGGGAGCAACCTTTTTCCTGCTTTTGTTAACGTAACCTTGCGAGTTg          <  1:816935/140‑1 (MQ=255)                                                                                                                            cTAACGTCACCATGCCACGATGATAAGCATTCATATCACGAATATTAAAAAGCGTTTCATCGAATTTCTTTATTAATTCCCGCGCCTCCGGGAGCAACCTTTTTCCTGCTTTTGTTAACGTAACCTTGCGAGTTGTACGc     <  1:68387/140‑1 (MQ=255)                                                                                                                              aaCGTCACCATGCCACGATGATAAGCATTCATATCACGAATATTAAAAAGCGTTTCATCGAATTTCTTTATTAGTTCCCGCGCCTCCGGGAGCAACCTTTTTCCTGCTTTTGTTAACGTAACCTTGCGAGTTGTACGCTc   >  2:842267/1‑140 (MQ=255)                                                                                                                              aaCGTCACCATGCCACGATGATAAGCATTCATATCACGAATATTAAAAAGCGTTTCATCGAATTTCTTTATTAATTCCCGCGCCTCCGGGAGCAACCTTTTTCCTGCTTTTGTTAACGTAACCTTGCGAGTTGTACGCTc   >  1:6499/1‑140 (MQ=255)                                                                                                                              aaCGTCACCATGCCACGATGATAAGCATTCATATCACGAATATTAAAAAGCGTTTCATCGAATTTCTTTATTAATTCCCGCGCCTCCGGGAGCAACCTTTTTCCTGCTTTTGTTAACGTAACCTTGCGAGTTGTACGCTc   >  1:960123/1‑140 (MQ=255)                                                                                                                              aaCGTCACCATGCCACGATGATAAGCATTCATATCACGAATATTAAAAAGCGTTTCATCGAATTTCTTTATTAATTCCCGCGCCTCCGGGAGCAACCTTTTTCCTGCTTTTGTTAACGTAACCTTGCGAGTTGTACGCTc   >  2:823954/1‑140 (MQ=255)                                                                                                                               aCGTCACCATGCCACGATGATAAGCATTCATATCACGAATATTAAAAAGCGTTTCATCGAATTTCTTTATTAATTCCCGCGCCTCCGGGAGCAACCTTTTTCCTGCTTTTGTTAACGTAACCTTGCGAGTTGTACGCTCa  <  1:819828/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                  GCACCGATTCCATGCAATTATTCGTGCCTTGTTCCAGAATCCGCACTTTAATATTGGGATACAGCTCATTAAATTTACCGATTGCCAGCGGTAAAAAATAAAATACTGCGGTCGGAATACACGCTAACGTCACCATACCACGATGATAAGCATTCATATCACGAATATTAAAAAGCGTTTCATCGAATTTCTTTATTAATTCCCGCGCCTCCGGGAGCAACCTTTTTCCTGCTTTTGTTAACGTAACCTTGCGAGTTGTACGCTCA  >  NC_000913/800161‑800426

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑