Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	895,717	A→G	T243A (ACC→GCC)	potG →	putrescine ABC transporter ATP binding subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	895,717	0	A	G	100.0%	146.6 / NA	43	T243A (ACC→GCC)	potG	putrescine ABC transporter ATP binding subunit
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (20/23);  total (20/23)

CGCGTCGGTGTGACTTGTGTGATGGTCACCCACGATCAGGAAGAGGCGATGACCATGGCGGGGCGCATCGCCATTATGAATCGTGGGAAATTTGTCCAGATTGGCGAACCGGAAGAGATCTACGAGCATCCGACTACCCGCTATAGCGCCGAATTTATTGGCTCGGTAAACGTCTTTGAAGGCGTACTCAAAGAGCGTCAGGAAGATGGCCTGGTGCTTGATTCGCCGGGGCTGGTGCATCCACTGAAAGTCGACGCGGATGCCTCGGT  >  NC_000913/895582‑895850                                                                                                                                        |                                                                                                                                      cgcgTCGGTGTGACTTGTGTGATGGTCACCCACGATCAGGAAGAGGCGATGACCATGGCGGGGCGCATCGCCATTATGAATCGTGGGAAATTTGTCCAGATTGGCGAACCGGAAGAGATCTACGAGCATCCGACTGCCCg                                                                                                                                   >  1:104817/1‑140 (MQ=255)   cgTCGGTGTGACTTGTGTGATGGTCACCCACGATCAGGAAGAGGCGATGACCATGGCGGGGCGCATCGCCATTATGAATCGTGGGAATTTTGTCCAGATTGGCGAACCGGAAGAGATCTACGAGCATCCGACTGCCCGCt                                                                                                                                 >  1:241385/1‑140 (MQ=255)   cgTCGGTGTGACTTGTGTGATGGTCACCCACGATCAGGAAGAGGCGATGACCATGGCGGGGCGCATCGCCATTATGAATCGTGGGAAATTTGTCCAGATTGGCGAACCGGAAGAGATCTACGGGCATCCGACTGCCCGCt                                                                                                                                 >  1:536824/1‑140 (MQ=255)   cgTCGGTGTGACTTGTGTGATGGTCACCCACGATCAGGAAGAGGCGATGACCATGGCGGGGCGCATCGCCATTATGAATCGTGGGAAATTTGTCCAGATTGGCGAACCGGAAGAGATCTACGAGCATCCGACTGCCCGCt                                                                                                                                 >  1:347863/1‑140 (MQ=255)    gTCGGTGTGACTTGTGTGATGGTCACCCACGATCAGGAAGAGGCGATGACCATGGCGGGGCGCATCGCCATTATGAATCGTGGGAAATTTGTCCAGATTGGCGAACCGGAAGAGATCTACGAGCATCCGACTGCCCGCta                                                                                                                                <  2:542779/140‑1 (MQ=255)          gtgACTTGTGTGATGGTCACCCACGATCAGGAAGAGGCGATGACCATGGCGGGGCGCATCGCCATTATGAATCGTGGGAAATTTGTCCAGATTGGCGAACCGGAAGAGATCTACGAGCATCCGACTGCCCGCTATAgcgc                                                                                                                          <  2:521494/140‑1 (MQ=255)                  tgtgATGGTCACCCACGATCAGGAAGAGGCGATGACCATGGCGGGGCGCATCGCCATTATGAATCGTGGGAAATTTGTCCAGATTGGCGAACCGGAAGAGATCTACGAGCATCCGACTGCCCGCTATAGCGCCGAATTTa                                                                                                                  <  2:455463/140‑1 (MQ=255)                      aTGGTCACCCACGATCAGGAAGAGGCGATGACCATGGCGGGGCGCATCGCCATTATGAATCGTGGGAAATTTGTCCAGATTGGCGAACCGGAAGAGATCTACGGGCATCCGACTGCCCGCTATAGCGCCGAATTTATTgg                                                                                                              <  2:536824/140‑1 (MQ=255)                       tGGTCACCCACGATCAGGAAGAGGCGATGACCATGGCGGGGCGCATCGCCATTATGAATCGTGGGAAATTTGTCCAGATTGGCGAACCGGAAGAGATCTACGAGCATCCGACTGCCCGCTATAGCGCCGAATTTATTGGc                                                                                                             <  1:333337/140‑1 (MQ=255)                       tGGTCACCCACGATCAGGAAGAGGCGATGACCATGGCGGGGCGCATCGCCATTATGAATCGTGGGAAATTTGTCCAGATTGGCGAACCGGAAGAGAACTACGAGCATCCGACTGCCCGCTATAGCGCCGAATATATTGGc                                                                                                             <  2:223790/140‑1 (MQ=255)                        ggTCACCCACGATCAGGAAGAGGCGATGACCATGGCGGGGCGCATCGCCATTATGAATCGTGGGAAATTTGTCCAGATTGGCGAACCGGAAGAGATCTACGAGCATCCGACTGCCCGCTATAGCGCCGAATTTATTGGCt                                                                                                            <  2:934558/140‑1 (MQ=255)                            aCCCACGATCAGGAAGAGGCGATGACCATGGCGGGGCGCATCGCCATTATGAATCGTGGGAAATTTGTCCAGATTGGCGAACCGGAAGAGATCTACGAGCATCCGACTGCCCGCTATAGCGCCGAATTTATTGGCTCGGt                                                                                                        >  2:170623/1‑140 (MQ=255)                            aCCCACGATCAGGAAGAGGCGATGACCATGGCGGGGCGCATCGCCATTATGAATCGTGGGAAATTTGTCCAGATTGGCGAACCGGAAGAGATCTACGAGCATCCGACTGCCCGCTATAGCGCCGAATTTATTGGCTCGGt                                                                                                        >  2:414554/1‑140 (MQ=255)                               cACGATCAGGAAGAGGCGATGACCATGGCGGGGCGCATCGCCATTATGAATCGTGGGAAATTTGTCCAGATTGGCGAACCGGAAGAGATCTACGAGCATCCGACTGCCCGCTATAGCGCCGAATTTATTGGCTCGGTaaa                                                                                                     <  1:405229/140‑1 (MQ=255)                                   aTCAGGAAGAGGCGATGACCATGGCGGGGCGCATCGCCATTATGAATCGTGGGAAATTTGTCCAGATTGGCGAACCGGAAGAGATCTACGAGCATCCGACTGCCCGCTATAGCGCCGAATTTATTGGCTCGGTAAACGTc                                                                                                 >  2:161565/1‑140 (MQ=255)                                        gAAGAGGCGATGACCATGGCGGGGCGCATCGCCATTATGAATCGTGGGAAATTTGTCCAGATTGGCGAACCGGAAGAGATCTACGAGCATCCGACTGCCCGCTATAGCGCCGAATTTATTGGCTCGGTAAAAATCTTTGa                                                                                            >  2:461037/1‑140 (MQ=255)                                           gagGCGATGACCATGGCGGGGCGCATCGCCATTATGAATCGTGGGAAATTTGTCCAGATTGGCGAACCGGAAGAGATCTACGAGCATCCGACTGCCCGCTATAGCGCCGAATTTATTGGCTCGGTAAACGTCTTTGAAgg                                                                                         <  2:967605/140‑1 (MQ=255)                                           gagGCGATGACCATGGCGGGGCGCATCGCCATTATGAATCGTGGGAAATTTGTCCAGATTGGCGAACCGGAAGAGATCTACGAGCATCCGACTGCCCGCTATAGCGCCGAATTTATTGGCTCGGTAAACGTCTTTGAAgg                                                                                         <  1:414554/140‑1 (MQ=255)                                            agGCGATGACCATGGCGGGGCGCATCGCCATTATGAATCGTGGGAAATTTGTCCAGATTGGCGAACCGGAAGAGATCTACGAGCATCCGACTGCCCGCTATAGCGCCGAATTTATTGGCTCGGTAAACGTCTTTGAAGGc                                                                                        >  1:398147/1‑140 (MQ=255)                                            agGCGATGACCATGGCGGGGCGCATCGCCATTATGAATCGTGGGAAATTTGTCCAGATTGGCGAACCGGAAGAGATCTACGAGCATCCGACTGCCCGCTATAGCGCCGAATTTATTGGCTCGGTAAACGTCTTTGAAGGc                                                                                        >  1:798176/1‑140 (MQ=255)                                              gCGATGACCATGGCGGGGCGCATCGCCATTATGAATCGTGGGAAATTTGTCCAGATTGGCGAACCGGAAGAGATCTACGAGCATCCGACTGCCCGCTATAGTGCCGAATTTATTGGCTCGGTAAACGTCTTTGAAGGCGt                                                                                      >  1:861130/1‑140 (MQ=255)                                              gCGATGACCATGGCGGGGCGCATCGCCATTATGAATCGTGGGAAATTTGTCCAGATTGGCGAACCGGAAGAGATCTACGAGCATCCGACTGCCCGCTATAGCGCCGAATTTATTGGCTCGGTAAACGTCTTTGAAGGCGt                                                                                      >  1:926260/1‑140 (MQ=255)                                                       aTGGCGGGGCGCATCGCCATTATGAATCGTGGGAAATTTGTCCAGATTGGCGAACCGGAAGAGATCTACGAGCATCCGACTGCCCGCTATAGCGCCGAATTTATTGGCTCGGTAAACGTCTTTGAAGGCGTACTCAAaga                                                                             <  2:926260/140‑1 (MQ=255)                                                              ggCGCATCGCCATTATGAATCGTGGGAAATTTGTCCAGATTGGCGAACCGGAAGAGATCTACGAGCATCCGACTGCCCGCTATAGCGCCGAATTTATTGGCTCGGTAAACGTCTTTGAAGGCGTACTCAAAGAGCGTCAg                                                                      >  1:149854/1‑140 (MQ=255)                                                                    tCGCCATTATGAATCGTGGGAAATTTGTCCAGATTGGCGAACCGGAAGAGATCTACGAGCATCCGACTGCCCGCTATAGCGCCGAATTTATTGGCTCGGTAAACGTCTTTGAAGGCGTACTCAAAGAGc                                                                           <  2:107850/129‑1 (MQ=255)                                                                    tCGCCATTATGAATCGTGGGAAATTTGTCCAGATTGGCGAACCGGAAGAGATCTACGAGCATCCGACTGCCCGCTATAGCGCCGAATTTATTGGCTCGGTAAACGTCTTTGAAGGCGTACTCAAAGAGc                                                                           >  1:107850/1‑129 (MQ=255)                                                                    tCGCCATTATGAATCGTGGGAAATTTGTCCAGATTGGCGAACCGGAAGAGATCTACGAGCATCCGACTGCCCGCTATAGCGCCGAATTTATTGGCTCGGTAAACGTCTTTGAAGGCGTACTCAAAGAGCGTCAGGAAGAt                                                                <  2:149854/140‑1 (MQ=255)                                                                       ccATTATGAATCGTGGGAAATTTGTCCAGATTGGCGAACCGGAAGAGATCTACGAGCATCCGACTGCCCGCTATAGCGCCGAATTTATTGGCTCGGTAAACGTCTTTGAAGGCGTACTCAAAGAGCGTCAGGAAGATGGc                                                             <  2:128412/140‑1 (MQ=255)                                                                       ccATTATGAATCGTGGGAAATTTGTCCAGATTGGCGAACCGGAAGAGATCTACGAGCATCCGACTGCCCGCTATAGCGCCGAATTTATTGGCTCGGTAAACGTCTTTGAAGGCGTACTCAAAGAGCGTCAGGAAGATGGc                                                             <  1:170623/140‑1 (MQ=255)                                                                        cATTATGAATCGTGGGAAATTTGTCCAGATTGGCGAACCGGAAGAGATCTACGAGCATCCGACTGCCCGCTATAGCGCCGAATTTATTGGCTCGGTAAACGTCTTTGAAGGCGTACTCAAAGAGCGTCAGGAAGATGGcc                                                            <  1:498560/140‑1 (MQ=255)                                                                        cATTATGAATCGTGGGAAATTTGTCCAGATTGGCGAACCGGAAGAGATCTACGAGCATCCGACTGCCCGCTATAGCGCCGAATTTATTGGCTCGGTAAACGTCTTTGAAGGCGTACTCAAAGAGCGTCAGGAAGATGGcc                                                            <  1:161565/140‑1 (MQ=255)                                                                          ttATGAATCGTGGGAAATTTGTCCAGATTGGCGAACCGGAAGAGATCTACGAGCATCCGACTGCCCGCTATAGTGCCGAATTTATTGGCTCGGTAAACGTCTTTGAAGGCGTACTCAAAGAGCGTCAGGAAGATGGCCTg                                                          <  2:861130/140‑1 (MQ=255)                                                                               aaTCGTGGGAAATTTGTCCAGATTGGCGAACCGGAAGAGATCTACGAGCATCCGACTGCCCGCTATAGCGCCGAATTTATTGGCTCGGTAAACGTCTTTGAAGGCGTACTCAAAGAGCGTCAGGAAGATGGcc                                                            <  2:173465/133‑1 (MQ=255)                                                                               aaTCGTGGGAAATTTGTCCAGATTGGCGAACCGGAAGAGATCTACGAGCATCCGACTGCCCGCTATAGCGCCGAATTTATTGGCTCGGTAAACGTCTTTGAAGGCGTACTCAAAGAGCGTCAGGAAGATGGcc                                                            >  1:173465/1‑133 (MQ=255)                                                                                 tCGTGGGAAATTTGTCCAGATTGGCGAACCGGAAGAGATCTACGAGCATCCGACTGCCCGCTATAGCGCCGAATTTATTGGCTCGGTAAACGTCTTTGAAGGCGTACTCAAAGAGCGTCAGGAAGATGGCCTGGTGCTTg                                                   >  1:731195/1‑140 (MQ=255)                                                                                  cGTGGGAAATTTGTCCAGATTGGCGAACCGGAAGAGATCTACGAGCATCCGACTGCCCGCTATAGCGCCGAATTTATTGGCTCGGTAAACGTCTTTGAAGGCGTACTCAAAGAGCGTCAGGAAGATGGCCTGGTGCTTGa                                                  <  2:347863/140‑1 (MQ=255)                                                                                  cGTGGGAAATTTGTCCAGATTGGCGAACCGGAAGAGATCTACGAGCATCCGACTGCCCGCTATAGCGCCGAATTTATTGGCTCGGTAAACATCTTTGAAGGCGTACTCAAAGAGCGTCAGGAAGATGGCCTGGTGCTTGa                                                  <  1:461037/140‑1 (MQ=255)                                                                                    tGGGAAATTTGTCCAGATTGGCGAACCGGAAGAGATCTACGAGCATCCGACTGCCCGCTATAGCGCCGAATTTATTGGCTCGGTAAACGTCTTTGAAGGCGTACTCAAAGAGCGTCAGGAAGATGGCCTGGTGCTTGAtt                                                >  2:702516/1‑140 (MQ=255)                                                                                            ttGTCCAGATTGGCGAACCGGAAGAGATCTACGAGCATCCGACTGCCCGCTATAGCGCCGAATTTATTGGCTCGGTAAACGTCTTTGAAGGCGTACTCAAAGAGCGTCAGGAAGATGGCCTGGTGCTTGATTCGCCgggg                                        <  2:731195/140‑1 (MQ=255)                                                                                                         cGAACCGGAAGAGATCTACGAGCATCCGACTGCCCGCTATAGCGCCGAATTTATTGGCTCGGTAAACGTCTTTGAAGGCGTACTCAAAGAGCGTCAGGAAGATGGCCTGGTGCTTGATTCGc                                             >  1:819379/1‑122 (MQ=255)                                                                                                         cGAACCGGAAGAGATCTACGAGCATCCGACTGCCCGCTATAGCGCCGAATTTATTGGCTCGGTAAACGTCTTTGAAGGCGTACTCAAAGAGCGTCAGGAAGATGGCCTGGTGCTTGATTCGc                                             <  2:819379/122‑1 (MQ=255)                                                                                                                      aTCTACGAGCATCCGACTGCCCGCTATAGCGCCGAATTTATTGGCTCGGTAAACGTCTTTGAAGGCGTACTCAAAGAGCGTCAGGAAGATGGCCTGGTGCTTGATTCGCCGGGGCTGGTGCATCCACTGAAAGTCGAcgc              >  1:43439/1‑140 (MQ=255)                                                                                                                         tACGAGCATCCGACTGCCCGCTATAGCGCCGAATTTATTGGCTCGGTAAACGTCTTTGAAGGCGTACTCAAAGAGCGTCAGGAAGATGGCCTGGTGCTTGATTCGCCGGGGCTGGTGCATCCACTGAAAGTCGACGCGGa           <  1:702516/140‑1 (MQ=255)                                                                                                                                aTCCGACTGCCCGCTATAGCGCCGAATTTATTGGCTCGGTAAACGTCTTTGAAGGCGTACTCAAAGAGCGTCAGGAAGATGGCCTGGTGCTTGATTCGCCGGGGCTGGTGCATCCACTGAAAGTCGACGCGGATGCCTCg    >  1:341850/1‑140 (MQ=255)                                                                                                                                  ccGACTGCCCGCTATAGCGCCGAATTTATTGGCTCGGTAAACGTCTTTGAAGGCGTACTCAAAGAGCGTCAGGAAGATGGCCTGGTGCTTGATTCGCCGGGGCTGGTGCATCCACTGAAAGTCGACGCGGATGCCTCggg  >  2:230575/1‑139 (MQ=255)                                                                                                                                        |                                                                                                                                      CGCGTCGGTGTGACTTGTGTGATGGTCACCCACGATCAGGAAGAGGCGATGACCATGGCGGGGCGCATCGCCATTATGAATCGTGGGAAATTTGTCCAGATTGGCGAACCGGAAGAGATCTACGAGCATCCGACTACCCGCTATAGCGCCGAATTTATTGGCTCGGTAAACGTCTTTGAAGGCGTACTCAAAGAGCGTCAGGAAGATGGCCTGGTGCTTGATTCGCCGGGGCTGGTGCATCCACTGAAAGTCGACGCGGATGCCTCGGT  >  NC_000913/895582‑895850

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑