Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,208,053 A→G T185A (ACA→GCA)  ycfK → e14 prophage; protein StfP

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,208,0530AG100.0% 171.2 / NA 48T185A (ACA→GCA) ycfKe14 prophage; protein StfP
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (22/26);  total (22/26)

CAAAAGCATCTGCCTCT‑‑GGGGGGGATGGAAATACAGTTGTATTCCCGGTTGCGTTTAATAATGCCTGTGTTGCCGTTGTTGCAAATTATGACAATGTCAGCGCACCTATCAATGCAGTGGCAACGGGGGGATATACAACCACTTCGTTTTTATTACGGTGCGCAGCTCAAACGGGTAGTTATTACTATAACTGGATTGCTATTGGGTATTAAGATGAAAATATACTGTTGCTTAAATACCGTTGGTTTTTTTATGGATGGCTGTGG  >  NC_000913/1207919‑1208184
                                                                                                                                        |                                                                                                                                   
cAAAAGCATCTGCCTCTGGGGGGGGGATGGAAATACAGTTGTATTCCCGGTTGCGTTTAATAATGCCTGTGTTGCCGTTGTTGCAAATTATGACAATGTCAGCGCACCTATCAATGCAGTGGCAACGGGGGGATATGCaa                                                                                                                                  >  1:173520/1‑140 (MQ=255)
cAAAAGCATCTGCCTCTGGGGGGGGGATGGAAATACAGTTGTATTCCCGGTTGCGTTTAATAATGCCTGTGTTGCCGTTGTTGCAAATTATGACAATGTCAGCGCACCTATCAATGCAGTGGCAACGGGGGGATATGCaa                                                                                                                                  >  2:332192/1‑140 (MQ=255)
         cTGCCTCTGGGGGGGGGATGGAAATACAGTTGTATTCCCGGTTGCGTTTAATAATGCCTGTGTTGCCGTTGTTGCAAATTATGACAATGTCAGCGCACCTATCAATGCAGTGGCAACGGGGGGATATGCAACCACTTCGt                                                                                                                         <  1:332192/140‑1 (MQ=255)
         cTGCCTCTGGGGGGGGGATGGAAATACAGTTGTATTCCCGGTTGCGTTTAATAATGCCTGTGTTGCCGTTGTTGCAAATTATGACAATGTCAGCGCACCTATCAATGCAGTGGCAACGGGGGGATATGCAACCACTTCGt                                                                                                                         <  1:765952/140‑1 (MQ=255)
                tgggggggggATGGAAATACAGTTGTATTCCCGGTTGCGTTTAATAATGCCTGTGTTGCCGTTGTTGCAAATTATGACAATGTCAGCGCACCTATCAATGCAGTGGCAACGGGGGGATATGCAACCACTTCGTTTttatt                                                                                                                  >  2:895321/4‑140 (MQ=255)
                      ggggATGGAAATACAGTTGTATTCCCGGTTGCGTTTAATAATGCCTGTGTTGCCGTTGTTGCAAATTATGACAATGTCAGCGCACCTATCAATGCAGTGGCAACGGGGGGATATGCAACCACTTCGTTTTTATTACGGTg                                                                                                            >  1:778548/1‑140 (MQ=255)
                       gggATGGAAATACAGTTGTATTCCCGGTTGCGTTTAATAATGCCTGTGTTGCCGTTGTTGCAAATTATGACAATGTCAGCGCACCTATCAATGCAGTGGCAACGGGGGGATATGCAACCACTTCGTTTTTATTACGGTgc                                                                                                           >  1:194048/1‑140 (MQ=255)
                        ggATGGAAATACAGTTGTATTCCCGGTTGCGTTTAATAATGCCTGTGTTGCCGTTGTTGCAAATTATGACAATGTCAGCGCACCTATCAATGCAGTGGCAACGGGGGGGTATGCAACCACTTCGTTTTTATTACGGTgcg                                                                                                          >  1:960986/1‑140 (MQ=255)
                          aTGGAAATACAGTTGTATTCCCGGTTGCGTTTAATAATGCCTGTGTTGCCGTTGTTGCAAATTATGACAATGTCAGCGCACCTATCAATGCAGTGGCAACGGGGGGATATGCAACCACTTCGTTTTTATTACGGTGCGCa                                                                                                        >  2:816094/1‑140 (MQ=255)
                           tGGAAATACAGTTGTATTCCCGGTTGCGTTTAATAATGCCTGTGTTGCCGTTGTTGCAAATTATGACAATGTCAGCGCACCTATCAATGCAGTGGCAACGGGGGGATATGCAACCACTTCGTTTTTATTACGGTGCGCAg                                                                                                       >  2:285700/1‑140 (MQ=255)
                           tGGAAATACAGTTGTATTCCCGGTTGCGTTTAATAATGCCTGTGTTGCCGTTGTTGCAAATTATGACAATGTCAGCGCACCTATCAATGCAGTGGCAACGGGGGGATATGCAACCACTTCGTTTTTATTACGGTGCGCAg                                                                                                       <  1:895321/140‑1 (MQ=255)
                            ggAAATACAGTTGTATTCCCGGTTGCGTTTAATAATGCCTGTGTTGCCGTTGTTGCAAATTATGACAATGTCAGCGCACCTATCAATGCAGTGGCAACGGGGGGATATGCAACCACTTCGTTTTTATTACGGTgcg                                                                                                          >  2:383611/1‑136 (MQ=255)
                            ggAAATACAGTTGTATTCCCGGTTGCGTTTAATAATGCCTGTGTTGCCGTTGTTGCAAATTATGACAATGTCAGCGCACCTATCAATGCAGTGGCAACGGGGGGATATGCAACCACTTCGTTTTTATTACGGTgcg                                                                                                          <  1:383611/136‑1 (MQ=255)
                             gAAATACAGTTGTATTCCCGGTTGCGTTTAATAATGCCTGTGTTGCCGTTGTTGCAAATTATGACAATGTCAGCGCACCTATCAATGCAGTGGCAACGGGGGGATATGCAACCACTTCGTTTTTATTACGGTGCGCAGCt                                                                                                     <  2:173520/140‑1 (MQ=255)
                                 tACAGTTGTATTCCCGGTTGCGTTTAATAATGCCTGTGTTGCCGTTGTTGCAAATTATGACAATGTTAGCGCACCTATCAATGCAGTGGCAACGGGGGGATATGCAACCACTTCGTTTTTATTACGGTGCGCAGCTCaaa                                                                                                 <  1:26588/140‑1 (MQ=255)
                                    aGTTGTATTCCCGGTTGCGTTTAATAATGCCTGTGTTGCCGTTGTTGCAAATTATGACAATGTCAGCGCACCTATCAATGCAGTGGCAACGGGGGGATATGCAACCACTTCGTTTTTATTACGGTGCGCAGCTCAAACgg                                                                                              <  2:960986/140‑1 (MQ=255)
                                     gTTGTATTCCCGGTTGCGTTTAATAATGCCTGTGTTGCCGTTGTTGCAAATTATGACAATGTCAGCGCACCTATCAATGCAGTGGCAACGGGGGGATATGCAACCACTTCGTTTTTATTACGGTGCGCAGCTCAAACggg                                                                                             <  1:285700/140‑1 (MQ=255)
                                     gTTGTATTCCCGGTTGCGTTTAATAATGCCTGTGTTGCCGTTGTTGCAAATTATGACAATGTCAGCGCACCTATCAATGCAGTGGCAACGGGGGGATATGCAACCACTTCGTTTTTATTACGGTGCGCAGCTCAAACggg                                                                                             >  2:906612/1‑140 (MQ=255)
                                      ttGTATTCCCGGTTGCGTTTAATAATGCCTGTGTTGCCGTTGTTGCAAATTATGACAATGTCAGCGCACCTATCAATGCAGTGGCAACGGGGGGATATGCAACCACTTCGTTTTTATTACGGTGCGCAGCTCAAACGGGt                                                                                            <  1:816094/140‑1 (MQ=255)
                                      ttGTATTCCCGGTTGCGTTTAATAATGCCTGTGTTGCCGTTGTTGCAAATTATGACAATGTCAGCGCACCTATCAATGCAGTGGCAACGGGGGGATATGCAACCACTTCGTTTTTATTACGGTGCGCAGCTCAAACGGGt                                                                                            >  2:195159/1‑140 (MQ=255)
                                       tGTATTCCCGGTTGCGTTTAATAATGCCTGTGTTGCCGTTGTTGCAAATTATGACAATGTCAGCGCACCTATCAATGCAGTGGCAACGGGGGGATATGCAACCACTTCGTTTTTATTACGGTGCGCAGCTCAAACGGGTa                                                                                           >  2:891014/1‑140 (MQ=255)
                                            tCCCGGTTGCGTTTAATAATGCCTGTGTTGCCGTTGTTGCAAATTATGACAATGTCAGCGCACCTATCAATGCAGTGGCAACGGGGGGATATGCAACCACTTCGTTTTTATTACGGTGCGCAGCTCAAACGGGTAGttat                                                                                      <  1:891014/140‑1 (MQ=255)
                                                ggTTGCGTTTAATAATGCCTGTGTTGCCGTTGTTGCAAATTATGACAATGTCAGCGCACCTATCAATGCAGTGGCAACGGGGGGATATGCAACCACTTCGTTTTTATTACGGTGCGCAGCTCAAACgg                                                                                              >  1:354313/1‑128 (MQ=255)
                                                ggTTGCGTTTAATAATGCCTGTGTTGCCGTTGTTGCAAATTATGACAATGTCAGCGCACCTATCAATGCAGTGGCAACGGGGGGATATGCAACCACTTCGTTTTTATTACGGTGCGCAGCTCAAACgg                                                                                              <  2:354313/128‑1 (MQ=255)
                                                   tGCGTTTAATAATGCCTGTGTTGACGTTGTTGCAAATTATGACAATGTCAGCGCACCTATCAATGCAGTGGCAACGGGGGGATATGCAACCACTTCGTTTTTATTACGGTGCGCAGCTCAAAC‑GGTAGTTATCACTATaa                                                                              >  2:571848/1‑140 (MQ=255)
                                                    gCGTTTAATAATGCCTGTGTTGCCGTTGTTGCAAATTATGACAATGTCAGCGCACCTATCAATGCAGTGGCAACGGGGGGATATGCAACCACTTCGTTTTTATTACGGTGCGCAGCTCAAACGGGTAGTTATTACTATaa                                                                              <  1:906612/140‑1 (MQ=255)
                                                    gCGTTTAATAATGCCTGTGTTGCCGTTGTTGCAAATTATGACAATGTCAGCGCACCTATCAATGCAGTGGCAACGGGGGGATATGCAACCACTTCGTTTTTATTACGGTGCGCAGCTCAAACGGGTAGTTATTACTATaa                                                                              <  1:828600/140‑1 (MQ=255)
                                                     cGTTTAATAATGCCTGTGTTGCCGTTGTTGCAAATTATGACAATGTCAGCGCACCTATCAATGCAGTGGCAACGGGGGGATATGCAACCACTTCGTTTTTATTACGGTGCGCAGCTCAAACGGGTAGTTATTACTATAAc                                                                             >  1:608682/1‑140 (MQ=255)
                                                          aataatGCCTGTGTTGCCGTTGTTGCAAATTATGACAATGTCAGCGCACCTATCAATGCAGTGGCAACGGGGGGATATGCAACCACTTCGTTTTTATTACGGTGCGCAGCTCAAACGGGTAGTTATTACTATAACTGGAt                                                                        <  1:543020/140‑1 (MQ=255)
                                                                       ttGCCGTTGTTGCAAATTATGACAATGTCAGCGCACCTATCAATGCAGTGGCAACGGGGGGATATGCAACCACTTCGTTTTTATTACGGTGCGCAGCTCAAACGGGTAGTTATTACTATAACTGGATTGCTATTGGGTAt                                                           >  1:301023/1‑140 (MQ=255)
                                                                       ttGCCGTTGTTGCAAATTATGACAATGTCAGCGCACCTATCAATGCAGTGGCAACGGGGGGATATGCAACCACTTCGTTTTTATTACGGTGCGCAGCTCAAACGGGTAGTTATTACTATAACTGGATTGCTATTGGGTAt                                                           <  2:608682/140‑1 (MQ=255)
                                                                          ccGTTGTTGCAAATTATGACAATGTCAGCGCACCTATCAATGCAGTGGCAACGGGGGGATATGCAACCACTTCGTTTTTATTACGGTGCGCAGCTCAAACGGGTAGTTATTACTATAACTGGATTGCTATTGGGTATTaa                                                        >  1:447695/1‑140 (MQ=255)
                                                                          ccGTTGTTGCAAATTATGACAATGTCAGCGCACCTATCAATGCAGTGGCAACGGGGGGATATGCAACCACTTCGTTTTTATTACGGTGCGCAGCTCAAACGGGTAGTTATTACTATAACTGGATTGCTATTGGGTATTaa                                                        >  2:653827/1‑140 (MQ=255)
                                                                            gttgttGCAAATTATGACAATGTCAGCGCACCTATCAATGCAGTGGCAACGGGGGGATATGCAACCACTTCGTTTTTATTACGGTGCGCAGCTCAAACGGGTAGTTATTACTATAACTGGATTGCTATTGGGTATTAAGa                                                      <  2:447695/140‑1 (MQ=255)
                                                                             ttgttgCAAATTATGACAATGTCAGCGCACCTATCAATGCAGTGGCAACGGGGGGATATGCAACCACTTCGTTTTTATTACGGTGCGCAGCTCAAACGGGTAGTTATTACTATAACTGGATTGCTATTGGGTATTAAGAt                                                     >  1:651887/1‑140 (MQ=255)
                                                                               gttgCAAATTATGACAATGTCAGCGCACCTATCAATGCAGTGGCAACGGGGGGATATGCAACCACTTCGTTTTTATTACGGTGCGCAGCTCAAACGGGTAGTTATTACTATAACTGGATTGCTATTGGGTATTAAGATGa                                                   <  1:653827/140‑1 (MQ=255)
                                                                               gttgCAAATTATGACAATGTCAGCGCACCTATCAATGCAGTGGCAACGGGGGGATATGCAACCACTTCGTTTTTATTACGGTGCGCAGCTCAAACGGGTAGTTATTACTATAACTGGATTGCTATTGGGTATTAAGATGa                                                   <  2:301023/140‑1 (MQ=255)
                                                                                 tgCAAATTATGACAATGTCAGCGCACCTATCAATGCAGTGGCAACGGGGGGATATGCAACCACTTCGTTTTTATTACGGTGCGCAGCTCAAACGGGTAGTTATTACTATAACTGGATTGCTATTGGGTATTAAGATGaaa                                                 <  1:195159/140‑1 (MQ=255)
                                                                                 tgCAAATTATGACAATGTCAGCGCACCTATCAATGCAGTGGCAACGGGGGGATATGCAACCACTTCGTTTTTATTACGGTGCGCAGCTCAAAC‑GGTAGTTATCACTATAACTGGATTGCTATTGGGTATTAAGATGaaaa                                                <  1:571848/140‑1 (MQ=255)
                                                                                       ttATGACAATGTCAGCGCACCTATCAATGCAGTGGCAACGGGGGGATATGCAACCACTTCGTTTTTATTACGGTGCGCAGCTCAAACGGGTAGTTATTACTATAACTGGATTGCTATTGGGTATTAAGATGAAAATATAc                                           <  2:174935/140‑1 (MQ=255)
                                                                                        tATGACAATGTCAGCGCACCTATCAATGCAGTGGCAACGGGGGGATATGCAACCACTTCGTTTTTATTACGGTGCGCAGCTCAAACGGGTAGTTATTACTATAACTGGATTGCTATTGGGTATTAAGATGAAAATATACt                                          <  2:778548/140‑1 (MQ=255)
                                                                                        tATGACAATGTCAGCGCACCTATCAATGCAGTGGCAACGGGGGGATATGCAACCACTTCGTTTTTATTACGGTGCGCAGCTCAAACGGGTAGTTATTACTATAACTGGATTGCTATTGGGTATTAAGATGAAAATATACt                                          <  2:16534/140‑1 (MQ=255)
                                                                                          tGACAATGTCAGCGCACCTATCAATGCAGTGGCAACGGGGGGATATGCAACCACTTCGTTTTTATTACGGTGCGCAGCTCAAACGGGTAGTTATTACTATAACTGGATTGCTATTGGGTATTAAGa                                                      <  2:3902/126‑1 (MQ=255)
                                                                                          tGACAATGTCAGCGCACCTATCAATGCAGTGGCAACGGGGGGATATGCAACCACTTCGTTTTTATTACGGTGCGCAGCTCAAACGGGTAGTTATTACTATAACTGGATTGCTATTGGGTATTAAGa                                                      >  1:3902/1‑126 (MQ=255)
                                                                                          tGACAATGTCAGCGCACCTATCAATGCAGTGGCAACGGGGGGATATGCAACCACTTCGTTTTTATTACGGTGCGCAGCTCAAACGGGTAGTTATTACTATAACTGGATTGCTATTGGGTATTAAGATGAAAATATACTGt                                        <  2:194048/140‑1 (MQ=255)
                                                                                                           cTATCAATGCAGTGGCAACGGGGGGATATGCAACCACTTCGTTTTTATTACGGTGCGCAGCTCAAACGGGTAGTTATTACTATAACTGGATTGCTATTGGGTATTAAGATGAAAATATACTGTTGCTTAAATACCGTTgg                       <  2:591208/140‑1 (MQ=255)
                                                                                                                            aCGGGGGGATATGCAACCACTTCGTTTTTATTACGGTGCGCAGCTCAAACGGGTAGTTATTACTATAACTGGATTGCTATTGGGTATTAAGATGAAAATATACTGTTGCTTAAATACCGTTGGTTTTTTTATGGATGGCt      >  2:659012/1‑140 (MQ=255)
                                                                                                                                ggggATATGCAACCACTTCGTTTTTATTACGGTGCGCAGCTCAAACGGGTAGTTATTACTATAACTGGATTGCTATTGGGTATTAAGATGAAAATATACTGTTGCTTAAATACCGTTGGTTTTTTTATGGATGGCTGTgg  >  2:205547/1‑140 (MQ=255)
                                                                                                                                        |                                                                                                                                   
CAAAAGCATCTGCCTCT‑‑GGGGGGGATGGAAATACAGTTGTATTCCCGGTTGCGTTTAATAATGCCTGTGTTGCCGTTGTTGCAAATTATGACAATGTCAGCGCACCTATCAATGCAGTGGCAACGGGGGGATATACAACCACTTCGTTTTTATTACGGTGCGCAGCTCAAACGGGTAGTTATTACTATAACTGGATTGCTATTGGGTATTAAGATGAAAATATACTGTTGCTTAAATACCGTTGGTTTTTTTATGGATGGCTGTGG  >  NC_000913/1207919‑1208184

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑