Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,342,321	A→G	E134G (GAA→GGA)	pyrF →	orotidine‑5'‑phosphate decarboxylase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,342,321	0	A	G	100.0%	206.7 / NA	57	E134G (GAA→GGA)	pyrF	orotidine‑5'‑phosphate decarboxylase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (28/29);  total (28/29)

AGCTGACTTAGGCGTGTGGATGGTGAATGTTCATGCCTCTGGTGGGGCGCGTATGATGACCGCAGCGCGTGAGGCACTGGTTCCGTTTGGCAAAGATGCACCGCTTTTGATTGCTGTGACAGTGTTGACCAGCATGGAAGCCAGCGACCTGGTCGATCTTGGCATGACACTGTCACCTGCAGATTATGCAGAACGTCTGGCGGCACTGACGCAAAAATGTGGCCTTGATGGTGTGGTGTGTTCTGCTCAGGAAGCTGTGCGCTTTAAACAGG  >  NC_000913/1342184‑1342455                                                                                                                                          |                                                                                                                                       aGCTGACTTAGGCGTGTGGATGGTGAATGTTCATGCCTCTGGTGGGGCGCGTATGATGACCGCAGCGCGTGAGGCACTGGTTCCGTTTGGCAAAGATGCACCGCTTTTGATTGCTGTGACAGTGTTGACCAGCATGGGAg                                                                                                                                      >  2:352733/1‑140 (MQ=255)       cTTAGGCGTGTGGATGGTGAATGTTCATGCCTCTGGTGGGGCGCGTATGATGACCGCAGCGCGTGAGGCACTGGTTCCGTTTGGCAAAGATGCACCGCTTTTGATTGCTGTGACAGTGTTGACCAGCATGGGAGCCAGCg                                                                                                                                >  1:842800/1‑140 (MQ=255)              gtgtGGATGGTGAATGTTCATGCCTCTGGTGGGGCGCGTATGATGACCGCAGCGCGTGAGGCACTGGTTCCGTTTGGCAAAGATGCACCGCTTTTGATTGCTGTGACAGTGTTGACCAGCATGGGAGCCAGCGACCTGGt                                                                                                                         <  1:352733/140‑1 (MQ=255)              gtgtGGATGGTGAATGTTCATGCCTCTGGTGGGGCGCGTATGATGACCGCAGCGCGTGAGGCACTGGTTCCGTTTGGCAAAGATGCACCGCTTTTGATTGCTGTGACAGTGTTGACCAGCATGGGAGCCAGCGACCTGGt                                                                                                                         <  1:63311/140‑1 (MQ=255)                gtgGATGGTGAATGTTCATGCCTCTGGTGGGGCGCGTATGATGACCGCAGCGCGTGAGGCACTGGTTCCGTTTGGCAAAGATGCACCGCTTTTGATTGCTGTGACAGTGTTGACCAGCATGGGAGCCAGCGACCTGGTCg                                                                                                                       >  1:842597/1‑140 (MQ=255)                gtgGATGGTGAATGTTCATGCCTCTGGTGGGGCGCGTATGATGACCGCAGCGCGTGAGGCACTGGTTCCGTTTGGCAAAGATGCACCGCTTTTGATTGCTGTGACAGTGTTGACCAGCATGGGAGCCAGCGACCTGGTCg                                                                                                                       >  1:824483/1‑140 (MQ=255)                 tgGATGGTGAATGTTCATGCCTCTGGTGGGGCGCGTATGATGACCGCAGCGCGTGAGGCACTGGTTCCGTTTGGCAAAGATGCACCGCTTTTGATTGCTGTGACAGTGTTGACCAGCATGGGAGCCAGCGACCTGGTCGa                                                                                                                      >  1:804874/1‑140 (MQ=255)                  ggATGGTGAATGTTCATGCCTCTGGTGGGGCGCGTATGATGACCGCAGCGCGTGAGGCACTGGTTCCGTTTGGCAAAGATGCACCGCTTTTGATTGCTGTGACAGTGTTGACCAGCATGGGAGCCAGCGACCTGGTCGAt                                                                                                                     <  1:191746/140‑1 (MQ=255)                  ggATGGTGAATGTTCATGCCTCTGGTGGGGCGCGTATGATGACCGCAGCGCGTGAGGCACTGGTTCCGTTTGGCAAAGATGCACCGCTTTTGATTGCTGTGACAGTGTTGACCAGCATGGGAGCCAGCGACCTGGTCGAt                                                                                                                     <  1:633846/140‑1 (MQ=255)                   gATGGTGAATGTTCATGCCTCTGGTGGGGCGCGTATGATGACCGCAGCGCGTGAGGCACTGGTTCCGTTTGGCAAAGATGCACCGCTTTTGATTGCTGTGACAGTGTTGACCAGCATGGGAGCCAGCGACCTGGTCGATc                                                                                                                    <  2:616369/140‑1 (MQ=255)                      ggTGAATGTTCATGCCTCTGGTGGGGCGCGTATGATGACCGCAGCGCGTGAGGCACTGGTTCCGTTTGGCAAAGATGCACCGCTTTTGATTGCTGTGACAGTGTTGACCAGCATGGGAGCCAGCGACCTGGTCGATCTTg                                                                                                                 >  2:673830/1‑140 (MQ=255)                       gTGAATGTTCATGCCTCTGGTGGGGCGCGTATGATGACCGCAGCGCGTGAGGCACTGGTTCCGTTTGGCAAAGATGCACCGCTTTTGATTGCTGTGACAGTGTTGACCAGCATGGGAGCCAGCGACCTGGTCGATCTTgg                                                                                                                <  2:824483/140‑1 (MQ=255)                          aaTGTTCATGCCTCTGGTGGGGCGCGTATGATGACCGCAGCGCGTGAGGCACTGGTTCCGTTTGGCAAAGATGCACCGCTTTTGATTGCTGTGACAGTGTTGACCAGCATGGGAGCCAGCGACCTGGTCGATCTTGGCAt                                                                                                             >  2:764897/1‑140 (MQ=255)                              ttCATGCCTCTGGTGGGGCGCGTATGATGACCGCAGCGCGTGAGGCACTGGTTCCGTTTGGCAAAGATGCACCGCTTTTGATTACTGTGACAGTGTTGACCAGCATGGGAGCCAGCGACCTGGTCGATCTTGGCATGaca                                                                                                         >  1:548716/1‑140 (MQ=255)                                  tGCCTCTGGTGGGGCGCGTATGATGACCGCAGCGCGTGAGGCACTGGTTCCGTTTGGCAAAGATGCACCGCTTTTGATTACTGTGACAGTGTTGACCAGCATGGGAGCCAGCGACCTGGTCGATCTTGGCATGACACTGt                                                                                                     <  2:548716/140‑1 (MQ=255)                                    ccTCTGGTGGGGCGCGTATGATGACCGCAGCGCGTGAGGCACTGGTTCCGTTTGGCAAAGATGCACCGCTTTTGATTGCTGTGACAGTGTTGACCAGCATGGGAGCCAGCGACCTGGTCGATCTTGGCATGACACTGTCa                                                                                                   >  2:528438/1‑140 (MQ=255)                                       ctGGTGGGGCGCGTATGATGACCGCAGCGCGTGAGGCACTGGTTCCGTTTGGCAAAGATGCACCGCTTTTGATTGCTGTGACAGTGTTGACCAGCATGGGAGCCAGCGACCTGGTCGATCTTGGCATGACACTGTCACCt                                                                                                <  2:408210/140‑1 (MQ=255)                                       ctGGTGGGGCGCGTATGATGACCGCAGCGCGTGAGGCACTGGTTCCGTTTGGCAAAGATGCACCGCTTTTGATTGCTGTGACAGTGTTGACCAGCATGGGAGCCAGCGACCTGGTCGATCTTGGCATGACACTGTCACCt                                                                                                <  1:868711/140‑1 (MQ=255)                                        tggtggGGCGCGTATGATGACCGCAGCGCGTGAGGCACTGGTTCCGTTTGGCAAAGATGCACCGCTTTTGATTGCTGTGACAGTGTTGACCAGCATGGGAGCCAGCGACCTGGTCGATCTTGGCATGACACTGt                                                                                                     <  1:532368/134‑1 (MQ=255)                                        tggtggGGCGCGTATGATGACCGCAGCGCGTGAGGCACTGGTTCCGTTTGGCAAAGATGCACCGCTTTTGATTGCTGTGACAGTGTTGACCAGCATGGGAGCCAGCGACCTGGTCGATCTTGGCATGACACTGt                                                                                                     >  2:532368/1‑134 (MQ=255)                                        tggtggGGCGCGTATGATGACCGCAGCGCGTGAGGCACTGGTTCCGTTTGGCAAAGATGCACCGCTTTTGATTGCTGTGACAGTGTTGACCAGCATGGGAGCCAGCGACCTGGTCGATCTTGGCATGACACTGTCACCTg                                                                                               <  2:287853/140‑1 (MQ=255)                                            ggggCGCGTATGATGACCGCAGCGCGTGAGGCACTGGTTCCGTTTGGCAAAGATGCACCGCTTTTGATTGCTGTGACAGTGTTGACCAGCATGGGAGCCAGCGACCTGGTCGATCTTGGCATGACACTGTCACCTGCAGa                                                                                           <  2:824760/140‑1 (MQ=255)                                               gcgcGTATGATGACCGCAGCGCGTGAGGCACTGGTTCCGTTTGGCAAAGATGCACCGCTTTTGATTGCTGTGACAGTGTTGACCAGCATGGGAGCCAGCGACCTGGTCGATCTTGGCATGACACTGTCACCTGCAGa                                                                                           <  2:450237/137‑1 (MQ=255)                                               gcgcGTATGATGACCGCAGCGCGTGAGGCACTGGTTCCGTTTGGCAAAGATGCACCGCTTTTGATTGCTGTGACAGTGTTGACCAGCATGGGAGCCAGCGACCTGGTCGATCTTGGCATGACACTGTCACCTGCAGa                                                                                           >  1:450237/1‑137 (MQ=255)                                               gcgcGTATGATGACCGCAGCGCGTGAGGCACTGGTTCCGTTTGGCAAAGATGCACCGCTTTTGATTGCTGTGACAGTGTTGACCAGCATGGGAGCCAGCGACCTGGGCGATCTTGGCATGACACTGTCACCTGCAGa                                                                                           <  1:761348/137‑1 (MQ=255)                                               gcgcGTATGATGACCGCAGCGCGTGAGGCACTGGTTCCGTTTGGCAAAGATGCACCGCTTTTGATTGCTGTGACAGTGTTGACCAGCATGGGAGCCAGCGACCTGGGCGATCTTGGCATGACACTGTCACCTGCAGa                                                                                           >  2:761348/1‑137 (MQ=255)                                                cgcgTATGATGACCGCAGCGCGTGAGGCACTGGTTCCGTTTGGCAAAGATGCACCGCTTTTGATTGCTGTGACAGTGTTGACCAGCATGGGAGCCAGCGACCTGGTCGATCTTGGCATGACACTGTCACCTGCAGATTAt                                                                                       >  1:927626/1‑140 (MQ=255)                                                cgcgTATGATGACCGCAGCGCGTGAGGCACTGGTTCCGTTTGGCAAAGATGCACCGCTTTTGATTGCTGTGACAGTGTTGACCAGCATGGGAGCCAGCGACCTGGTCGATCTTGGCATGACACTGTCACCTGCAGATTAt                                                                                       <  2:613458/140‑1 (MQ=255)                                                              gCAGCGCGTGAGGCACTGGTTCCGTTTGGCAAAGATGCACCGCTTTTGATTGCTGTGACAGTGTTGACCAGCATGGGAGCCAGCGACCTGGTCGATCTTGGCATGACACTGTCACCTGCAGATTATGCAGAACGTCTggc                                                                         >  2:848875/1‑140 (MQ=255)                                                               cAGCGCGTGAGGCACTGGTTCCGTTTGGCAAAGATGCACCGCTTTTGATTGCTGTGACAGTGTTGACCAGCATGGGAGCCAGCGACCTGGTCGATCTTGGCATGACACTGTCACCTGCAGATTATGCAGAACGTCTggcg                                                                        >  2:440767/1‑140 (MQ=255)                                                                                gTTCCGTTTGGCAAAGATGCACCGCTTTTGATTGCTGTGACAGTGTTGACCAGCATGGGAGCCAGCGACCTGGTCGATCTTGGCATGACACTGTCACCTGCAGATTATGCAGAACGTCTggcgg                                                                       >  2:663384/1‑124 (MQ=255)                                                                                gTTCCGTTTGGCAAAGATGCACCGCTTTTGATTGCTGTGACAGTGTTGACCAGCATGGGAGCCAGCGACCTGGTCGATCTTGGCATGACACTGTCACCTGCAGATTATGCAGAACGTCTggcgg                                                                       <  1:663384/124‑1 (MQ=255)                                                                                    cGTTTGGCAAAGATGCACCGCTTTTGATTGCTGTGACAGTGTTGACCAGCATGCGAGCCAGCGACCTGGTCGATCTTGGCATGACACTGTCACCTGCAGATTATGCAGAACGTCTGGCGGCACTGACGCAAAAATGTGGc                                                   <  2:842597/140‑1 (MQ=255)                                                                                      tttGGCAAAGATGCACCGCTTTTGATTGCTGTGACAGTGTTGACCAGCATGGGAGCCAGCGACCTGGTCGATCTTGGCATGACACTGTCACCTGCAGATTATGCAGAACGTCTGGCGGCACTGACGCAAAAAtgt                                                      >  2:541322/1‑135 (MQ=255)                                                                                      tttGGCAAAGATGCACCGCTTTTGATTGCTGTGACAGTGTTGACCAGCATGGGAGCCAGCGACCTGGTCGATCTTGGCATGACACTGTCACCTGCAGATTATGCAGAACGTCTGGCGGCACTGACGCAAAAAtgt                                                      <  1:541322/135‑1 (MQ=255)                                                                                      tttGGCAAAGATGCACCGCTTTTGATTGCTGTGACAGTGTTGACCAGCATGGGAGCCAGCGACCTGGTCGATCTTGGCATGACACTGTCACCTGCAGATTATGCAGAACGTCTGGCGGCACTGACGCAAAAATGTGGCCt                                                 <  1:848875/140‑1 (MQ=255)                                                                                         ggCAAAGATGCACCGCTTTTGATTGCTGTGACAGTGTTGACCAGCATGGGAGCCAGCGACCTGGTCGATCTTGGCATGACACTGTCACCTGCAGATTATGCAGAACGTCTGGCGGCACTGACGCAAAAATGTGGCCTTGa                                              >  2:529455/1‑140 (MQ=255)                                                                                          gCAAAGATGCACCGCTTTTGATTGCTGTGACAGTGTTGACCAGCATGGGAGCCAGCGACCTGGTCGATCTTGGCATGACACTGTCACCCGCAGATTATGCAGAACGTCTGGCGGCACTGACGCAAAAATGTGGc                                                   <  2:177993/134‑1 (MQ=255)                                                                                          gCAAAGATGCACCGCTTTTGATTGCTGTGACAGTGTTGACCAGCATGGGAGCCAGCGACCTGGTCGATCTTGGCATGACACTGTCACCCGCAGATTATGCAGAACGTCTGGCGGCACTGACGCAAAAATGTGGc                                                   >  1:177993/1‑134 (MQ=255)                                                                                           cAAAGATGCACCGCTTTTGATTGCTGTGACAGTGTTGACCAGCATGGGAGCCAGCGACCTGGTCGATCTTGGCATGACACTGTCACCTGCAGATTATGCAGAACGTCTGGCGGCACTGACGCAAAAATGTGGCCTTGAtg                                            >  1:692/1‑140 (MQ=255)                                                                                           cAAAGATGCACCGCTTTTGATTGCTGTGACAGTGTTGACCAGCATGGGAGCCAGCGACCTGGTCGATCTTGGCATGACACTGTCACCTGCAGATTATGCAGAACGTCTGGCGGCACTGACGCAAAAATGTGGCCTTGAtg                                            <  1:440767/140‑1 (MQ=255)                                                                                             aaGATGCACCGCTTTTGATTGCTGTGACAGTGTTGACCAGCATGGGAGCCAGCGACCTGGTCGATCTTGGCATGACACTGTCACCTGCAGATTATGCAGAACGTCTGGCGGCACTGACGCAAAAATGTGGCCTTGAtggt                                          <  1:803231/140‑1 (MQ=255)                                                                                                aTGCACCGCTTTTGATTGCTGTGACAGTGTTGACCAGCATGGGAGCCAGCGACCTGGTCGATCTTGGCATGACACTGTCACCTGCAGATTATGCAGAACGTCTGGCGGCACTGACGCAAAAAtg                                                       <  1:11559/124‑1 (MQ=255)                                                                                                aTGCACCGCTTTTGATTGCTGTGACAGTGTTGACCAGCATGGGAGCCAGCGACCTGGTCGATCTTGGCATGACACTGTCACCTGCAGATTATGCAGAACGTCTGGCGGCACTGACGCAAAAAtg                                                       >  2:11559/1‑124 (MQ=255)                                                                                                  gCACCGCTTTTGATTGCTGTGACAGTGTTGACCAGCATGGGAGCCAGCGACCTGGTCGATCTTGGCATGACACTGTCACCTGCAGATTATGCAGAACGTCTGGCGGCACTGACGCAAAAATGTGGCCTTGAtggtgtggt                                     <  2:842800/140‑1 (MQ=255)                                                                                                       gCTTTTGATTGCTGTGACAGTGTTGACCAGCATGGGAGCCAGCGACCTGGTCGATCTTGGCATGACACTGTCACCTGCAGATTATGCAGAACGTCTGGCGGCACTGACGCAAAAATGTGGCCTTGATGGTGTGGTGTGtt                                <  2:468598/140‑1 (MQ=255)                                                                                                        cTTTTGATTGCTGTGACAGTGTTGACCAGCATGGGAGCCAGCGACCTGGTCGATCTTGGCATGACACTGTCACCTGCAGATTATGCAGAACGTCTGGCGGCACTGACGCAAAAATGTGGCCTTGATGGTGTGGTGTGTTc                               >  1:449098/1‑140 (MQ=255)                                                                                                                       aCAGTGTTGACCAGCATGGGAGCCAGCGACCTGGTCGATCTTGGCATGACACTGTCACCTGCAGATTATGCAGAACGTCTGGCGGCACTGACGCAAAAATGTGGCCTTGATGGTGTGGTGTGTTCTGCTCAGGAAGCtgt                >  2:239865/1‑140 (MQ=255)                                                                                                                                 ccAGCATGGGAGCCAGCGACCTGGTCGATCTTGGCATGACACTGTCACCTGCAGATTATGCAGAACGTCTGGCGGCACTGACGCAAAAATGTGGCCTTGATGGTGTGGTGTGTTCTGCTCAGGAAGCTGTGCGCTTTaaa      <  2:692/140‑1 (MQ=255)                                                                                                                                  cAGCATGGGAGCCAGCGACCTGGTCGATCTTGGCATGACACTGTCACCTGCAGATTATGCAGAACGTCTGGCGGCACTGACGCAAAAATGTGGCCTTGATGGTGTGGTGTGTTCTGCTCAGGAAGCTGTGCGCTTTaaa      >  2:429375/1‑139 (MQ=255)                                                                                                                                  cAGCATGGGAGCCAGCGACCTGGTCGATCTTGGCATGACACTGTCACCTGCAGATTATGCAGAACGTCTGGCGGCACTGACGCAAAAATGTGGCCTTGATGGTGTGGTGTGTTCTGCTCAGGAAGCTGTGCGCTTTaaa      <  1:429375/139‑1 (MQ=255)                                                                                                                                  cAGCATGGGAGCCAGCGACCTGGTCGATCTTGGCATGACACTGTCACCTGCAGATTATGCAGAACGTCTGGCGGCACTGACGCAAAAATGTGGCCTTGATGGTGTGGTGTGTTCTGCTCAGGAAGCTGTGCGCTTTAAAc     <  1:186602/140‑1 (MQ=255)                                                                                                                                     cATGGGAGCCAGCGACCTGGTCGATCTTGGCATGACACTGTCACCTGCAGATTATGCAGAACGTCTGGCGGCACTGACGCAAAAATGTGGCCTTGATGGTGTGGTGTGTTCTGCTCAGGAAGCTGTgcg             <  1:643534/129‑1 (MQ=255)                                                                                                                                     cATGGGAGCCAGCGACCTGGTCGATCTTGGCATGACACTGTCACCTGCAGATTATGCAGAACGTCTGGCGGCACTGACGCAAAAATGTGGCCTTGATGGTGTGGTGTGTTCTGCTCAGGAAGCTGTgcg             >  2:643534/1‑129 (MQ=255)                                                                                                                                     cATGGGAGCCAGCGACCTGGTCGATCTTGGCATGACACTGTCACCTGCAGATTATGCAGAACGTCTGGCGGCACTGACGCAAAAATGTGGCCTTGATGGTGTGGTGTGTTCTGCTCAGGAAGCTGTGCGCTTTAAACAgg  >  2:674515/1‑140 (MQ=255)                                                                                                                                     cATGGGAGCCAGCGACCTGGTCGATCTTGGCATGACACTGTCACCTGCAGATTATGCAGAACGTCTGGCGGCACTGACGCAAAAATGTGGCCTTGATGGTGTGGTGTGTTCTGCTCAGGAAGCTGTGCGCTTTAAACAgg  >  1:397438/1‑140 (MQ=255)                                                                                                                                     cATGGGAGCCAGCGACCTGGTCGATCTTGGCATGACACTGTCACCTGCAGATTATGCAGAACGTCTGGCGGCACTGACGCAAAAATGTGGCCTTGATGGTGTGGTGTGTTCTGCTCAGGAAGCTGTGCGCTTTAAACAgg  >  2:380811/1‑140 (MQ=255)                                                                                                                                          |                                                                                                                                       AGCTGACTTAGGCGTGTGGATGGTGAATGTTCATGCCTCTGGTGGGGCGCGTATGATGACCGCAGCGCGTGAGGCACTGGTTCCGTTTGGCAAAGATGCACCGCTTTTGATTGCTGTGACAGTGTTGACCAGCATGGAAGCCAGCGACCTGGTCGATCTTGGCATGACACTGTCACCTGCAGATTATGCAGAACGTCTGGCGGCACTGACGCAAAAATGTGGCCTTGATGGTGTGGTGTGTTCTGCTCAGGAAGCTGTGCGCTTTAAACAGG  >  NC_000913/1342184‑1342455

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑