Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,464,003	A→G	T155T (ACA→ACG)	paaX →	DNA‑binding transcriptional repressor PaaX

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,464,003	0	A	G	100.0%	188.3 / NA	52	T155T (ACA→ACG)	paaX	DNA‑binding transcriptional repressor PaaX
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (24/28);  total (24/28)

GCTCCTGTTGCTCTCGGAAGGTTTAGATAAATCAACGCTGGCTGATGTCAAAAAGCAGTTGATCTGGCAAGGTTTTGGCGCACTGGCACCCAGCCTGATGGCATCGCCGTCGCAAAAACTGGCCGATGTACAGACACTTTTGCATGAAGCGGGTGTGGCGGATAACGTGATTTGTTTTGAAGCGCAAATACCACTGGCGCTTTCTCGCGCAGCACTGCGTGCCAGAGTAGAAGAGTGCTGGCATTTAACTGAACAAAATGCCATGTACGA  >  NC_000913/1463868‑1464137                                                                                                                                        |                                                                                                                                       gCTCCTGTTGCTCTCGGAAGGTTTAGATAAATCAACGCTGGCTGATGTCAAAAAGCAGTTGATCTGGCAAGGTTTTGGCGCACTGGCACCCAGCCTGATGGCATCGCCGTCGCAAAAACTGGCCGATGTACAGACGCttt                                                                                                                                    >  2:459879/1‑140 (MQ=255)    ccTGTTGCTCTCGGAAGGTTTAGATAAATCAACGCTGGCTGATGTCAAAAAGCAGTTGATCTGGCAAGGTTTTGGCGCACTGGCACCCAGCCTGATGGCATCGCCGTCGCGAAAACTGGCCGATGTACAGACGCTTTTGc                                                                                                                                 >  1:548263/1‑140 (MQ=255)    ccTGTTGCTCTCGGAAGGTTTAGATAAATCAACGCTGGCTGATGTCAAAAAGCAGTTGATCTGGCAAGGTTTTGGCGCACTGGCACCCAGCCTGATGGCATCGCCGTCGCAAAAACTGGCCGATGTACAGACGCTTTTGc                                                                                                                                 >  2:369461/1‑140 (MQ=255)    ccTGTTGCTCTCGGAAGGTTTAGATAAATCAACGCTGGCTGATGTCAAAAAGCAGTTGATCTGGCAAGGTTTTGGCGCACTGGCACCCAGCCTGATGGCATCGCCGTCGCAAAAACTGGCCGATGTACAGACGCTTTTGc                                                                                                                                 >  2:756320/1‑140 (MQ=255)     cTGTTGCTCTCGGAAGGTTTAGATAAATCAACGCTGGCTGATGTCAAAAAGCAGTTGATCTGGCAAGGTTTTGGCGCACTGGCACCCAGCCTGATGGCATCGCCGTCGCAAAAACTGGCCGATGTACAGACGCTTTTGc                                                                                                                                 <  2:426749/139‑1 (MQ=255)     cTGTTGCTCTCGGAAGGTTTAGATAAATCAACGCTGGCTGATGTCAAAAAGCAGTTGATCTGGCAAGGTTTTGGCGCACTGGCACCCAGCCTGATGGCATCGCCGTCGCAAAAACTGGCCGATGTACAGACGCTTTTGc                                                                                                                                 >  1:426749/1‑139 (MQ=255)               cGGAAGGTTTAGATAAATCAACGCTGGCTGATGTCAAAAAGCAGTTGATCTGGCAAGGTTTTGGCGCACTGGCACCCAGCCTGATGGCATCGCCGTCGCAAAAACTGGCCGATGTACAGACGCTTTTGCa                                                                                                                                <  2:621402/130‑1 (MQ=255)               cGGAAGGTTTAGATAAATCAACGCTGGCTGATGTCAAAAAGCAGTTGATCTGGCAAGGTTTTGGCGCACTGGCACCCAGCCTGATGGCATCGCCGTCGCAAAAACTGGCCGATGTACAGACGCTTTTGCa                                                                                                                                >  1:621402/1‑130 (MQ=255)               cGGAAGGTTTAGATAAATCAACGCTGGCTGATGTCAAAAAGCAGTTGATCTGGCAAGGTTTTGGCGCACTGGCACCCAGCCTGATGGCATCGCCGTCGCAAAAACTGGCCGATGTACAGACGCTTTTGCATGAAGCGGgt                                                                                                                      >  2:67172/1‑140 (MQ=255)                     gTTTAGATAAATCAACGCTGGCTGATGTCAAAAAGCAGTTGATCTGGCAAGGTTTTGGCGCACTGGCACCCAGCCTGATGGCATCGCCGTCGCAAAAACTGGCCTATGTACAGACGCTTTTGCATGAAGCGGGTGTGGCg                                                                                                                >  1:870499/1‑140 (MQ=255)                     gTTTAGATAAATCAACGCTGGCTGATGTCAAAAAGCAGTTGATCTGGCAAGGTTTTGGCGCACTGGCACCCAGCCTGATGGCATCGCCGTCGCAAAAACTGGCCGATGTACAGACGCTTTTGCATGAAGCGGGTGTGGCg                                                                                                                >  2:369689/1‑140 (MQ=255)                           aTAAATCAACGCTGGCTGATGTCAAAAAGCAGTTGATCTGGCAAGGTTTTGGCGCACTGGCACCCAGCCTGATGGCATCGCCGTCGCAAAAACTGGCCGATGTACAGACGCTTTTGCATGAAGCGGGTGTGGCGGATAAc                                                                                                          <  1:84456/140‑1 (MQ=255)                              aaTCAACGCTGGCTGATGTCAAAAAGCAGTTGATCTGGCAAGGTTTTGGCGCACTGGCACCCAGCCTGATGGCATCGCCGTCGCGAAAACTGGCCGATGTACAGACGCTTTTGCATGAAGCGGGTGTGGCGGATAACGTg                                                                                                       <  2:548263/140‑1 (MQ=255)                               aTCAACGCTGGCTGATGTCAAAAAGCAGTTGATCTGGCAAGGTTTTGGCGCTCTGGCACCCAGCCTGATGGCATCGCCGTCGCAAAAACTGGCCGATGTACAGACGCTTTTGCATGAAGCGGGTGTGGCGGATAACGTGa                                                                                                      >  1:220965/1‑140 (MQ=255)                               aTCAACGCTGGCTGATGTCAAAAAGCAGTTGATCTGGCAAGGTTTTGGCGCACTGGCACCCAGCCTGATGGCATCGCCGTCGCAAAAACTGGCCGATGTACAGACGCTTTTGCATGAAGCGGGTGTGGCgg                                                                                                               <  2:519147/131‑1 (MQ=255)                               aTCAACGCTGGCTGATGTCAAAAAGCAGTTGATCTGGCAAGGTTTTGGCGCACTGGCACCCAGCCTGATGGCATCGCCGTCGCAAAAACTGGCCGATGTACAGACGCTTTTGCATGAAGCGGGTGTGGCgg                                                                                                               >  1:519147/1‑131 (MQ=255)                               aTCAACGCTGGCTGATGTCAAAAAGCAGTTGATCTGGCAAGGTTTTGGCGCACTGGCACCCAGCCTGATGGCATCGCCGTCGCAAAAACTGGCCGATGTACAGACGCTTTTGCATGAAGCGGGTGTGGCGGATAAc                                                                                                          <  1:611620/136‑1 (MQ=255)                               aTCAACGCTGGCTGATGTCAAAAAGCAGTTGATCTGGCAAGGTTTTGGCGCACTGGCACCCAGCCTGATGGCATCGCCGTCGCAAAAACTGGCCGATGTACAGACGCTTTTGCATGAAGCGGGTGTGGCGGATAAc                                                                                                          >  2:611620/1‑136 (MQ=255)                               aTCAACGCTGGCTGATGTCAAAAAGCAGTTGATCTGGCAAGGTTTTGGCGCACTGGCACCCAGCCTGATGGCATCGCCGTCGCAAAAACTGGCCGATGTACAGACGCTTTTGCATGAAGCGGGTGTGGCGGATAACGTGa                                                                                                      >  1:71783/1‑140 (MQ=255)                                tCAACGCTGGCTGATGTCAAAAAGCAGTTGATCTGGCAAGGTTTTGGCGCACTGGCACCCAGCCTGATGGCATCGCCGTCGCAAAAACTGGCCGATGTACAGACGCTTTTGCATGAAGCGGGTGTGGCGGATAACGTGAt                                                                                                     >  2:290258/1‑140 (MQ=255)                                         gctgATGTCAAAAAGCAGTTGATCTGGCAAGGTTTTGGCGCACTGGCACCCAGCCTGATGGCATCGCCGTCGCAAAAACTGGCCGATGTACAGACGCTTTTGCATGAAGCGGGTGTGGCGGATa                                                                                                            >  2:758695/1‑124 (MQ=255)                                         gctgATGTCAAAAAGCAGTTGATCTGGCAAGGTTTTGGCGCACTGGCACCCAGCCTGATGGCATCGCCGTCGCAAAAACTGGCCGATGTACAGACGCTTTTGCATGAAGCGGGTGTGGCGGATa                                                                                                            <  1:758695/124‑1 (MQ=255)                                          ctgATGTCAAAAAGCAGTTGATCTGGCAAGGTTTTGGCGCACTGGCACCCAGCCTGATGGCATCGCCGTCGCAAAAACTGGCCGATGTACAGACGCTTTTGCATGAAGCGGGTGTGGCGGATAACGTGATTTGTTTTGaa                                                                                           <  2:759723/140‑1 (MQ=255)                                            gATGTCAAAAAGCAGTTGATCTGGCAAGGTTTTGGCGCACTGGCACCCAGCCTGATGGCATCGCCGTCGCAAAAACTGGCCGATGTACAGACGCTTTTGCATGAAGCGGGTGTGGCGGATAACGTGATTTGTTTTGAAgc                                                                                         <  2:71783/140‑1 (MQ=255)                                            gATGTCAAAAAGCAGTTGATCTGGCAAGGTTTTGGCGCACTGGCACCCAGCCTGATGGCATCGCCGTCGCAAAAACTGGCCGATGTACAGACGCTTTTGCATGAAGCGGGTGTGGCGGATAACGTGATTTGTTTTGAAgc                                                                                         <  1:122308/140‑1 (MQ=255)                                               gTCAAAAAGCAGTTGATCTGGCAAGGTTTTGGCGCACTGGCACCCAGCCTGATGGCATCGCCGTCGCAAAAACTGGCCGATGTACAGACGCTTTTGCATGAAGCGGGTGTGGCGGATAACGTGATTTGTTTTGAAGCGCa                                                                                      <  1:684574/140‑1 (MQ=255)                                                            tGATCTGGCAAGGTTTTGGCGCACTGGCACCCAGCCTGATGGCATCGCCGTCGCAAAAACTGGCCGATGTACAGACGCTTTTGCATGAAGCGGGTGTGGCGGATAACGTGATTTGTTTTGAAGCGCAAATACCACTGgcg                                                                         <  1:774312/140‑1 (MQ=255)                                                                 tGGCAAGGTTTTGGCGCACTGGCACCCAGCCTGATGGCATCGCCGTCGCAAAAACTGGCCGATGTACAGACGCTTTTGCATGAAGCGGGTGTGGCGGATAACGTGATTTGTTTTGAAGCGCAAATACCACTGGCGCTTtc                                                                    <  1:369461/140‑1 (MQ=255)                                                                 tGGCAAGGTTTTGGCGCACTGGCACCCAGCCTGATGGCATCGCCGTCGCAAAAACTGGCCGATGTACAGACGCTTTTGCATGAAGAGGGTGTGGCGGATAACGTGATTTGTTTTGAAGCGCAAATACCACTGGCGCTTtc                                                                    <  1:756320/140‑1 (MQ=255)                                                                   gCAAGGTTTTGGCGCACTGGCACCCAGCCTGATGGCATCGCCGTCGCAAAAACTGGCCGATGTACAGACGCTTTTGCATGAAGCGGGTGTGGCGGATAACGTGATTTGTTTTGAAGCGCAAATACCACTGGCGCTTtctc                                                                  >  1:266514/1‑140 (MQ=255)                                                                      aGGTTTTGGCGCACTGGCACCCAGCCTGATGGCATCGCCGTCGCAAAAACTGGCCGATGTACAGACGCTTTTGCATGAAGCGGGTGTGGCGGATAACGTGATTTGTTTTGAAGCGCAAATACCACTGGCGCTTTCTcgcg                                                               <  2:391768/140‑1 (MQ=255)                                                                         ttttGGCGCACTGGCACCCAGCCTGATGGCATCGCCGTCGCAAAAACTGGCCGATGTACAGACGCTTTTGCATGAAGCGGGTGTGGCGGATAACGTGATTTGTTTTGAAGCGCAAATACCACTGGCGCTTTCTCGCgcag                                                            <  1:459879/140‑1 (MQ=255)                                                                             ggCGCACTGGCACCCAGCCTGATGGCATCGCCGTCGCAAAAACTGGCCGATGTACAGACGCTTTTGCATGAAGCGGGTGTGGCGGATAACGTGATTTTTTTTTAAACGCAAATAACACTGGCGCCTTTTCCCGCAGCACt                                                        >  2:452254/1‑140 (MQ=255)                                                                             ggCGCACTGGCACCCAGCCTGATGGCATCGCCGTCGCAAAAACTGGCCGATGTACAGACGCTTTTGCATGAAGCGGGTGTGGCGGATAACGTGATTTGTTTTGAAGCGCAAATACCACTGGCGCTTTCTCGCGCAGCACt                                                        <  1:452254/140‑1 (MQ=255)                                                                                gcACTGGCACCCAGCCTGATGGCATCGCCGTCGCAAAAACTGGCCGATGTACAGACGCTTTTGCATGAAGCGGGTGTGGCGGATAACGTGATTTGTTTTGAAGCGCAAATACCACTGGCGCTTTCTCGCGCAGCACTGCg                                                     <  1:67172/140‑1 (MQ=255)                                                                                   cTGGCACCCAGCCTGATGGCATCGCCGTCGCAAAAACTGGCCGATGTACAGACGCTTTTGCATGAAGCGGGTGTGGCGGATAACGTGATTTGTTTTGAAGCGCAAATACCACTGGCGCTTTCTCGCGCAGCACTGCGTGc                                                  >  1:924890/1‑140 (MQ=255)                                                                                     ggCACCCAGCCTGATGGCATCGCCGTCGCAAAAACTGGCCGATGTACAGACGCTTTTGCATGAAGCGGGTGTGGCGGATAACGTGATTTGTTTTGAAGCGCAAATACTACTGGCGCTTTCTCGCGCAGCACTGCGTGCCa                                                <  2:220965/140‑1 (MQ=255)                                                                                     ggCACCCAGCCTGATGGCATCGCCGTCGCAAAAACTGGCCGATGTACAGACGCTTTTGCATGAAGCGGGTGTGGCGGATAACGTGATTTGTTTTGAAGCGCAAATACCACTGGCGCTTTCTCGCGCAGCACTGCGTGCCa                                                <  2:924890/140‑1 (MQ=255)                                                                                     ggCACCCAGCCTGATGGCATCGCCGTCGCAAAAACTGGCCGATGTACAGACGCTTTTGCATGAAGCGGGTGTGGCGGATAACGTGATTTGTTTTGAAGCGCAAATACCACTGGCGCTTTCTCGCGCAGCACTGCGTGCCa                                                <  1:290258/140‑1 (MQ=255)                                                                                        aCCCAGCCTGATGGCATCGCCGTCGCAAAAACTGGCCGATGTACAGACGCTTTTGCATGAAGCGGGTGTGGCGGATAACGTGATTTGTTTTGAAGCGCAAATACCACTGGCGCTTTCTCGCGCAGCACTGCGTGCCagag                                             <  2:221066/140‑1 (MQ=255)                                                                                            aGCCTGATGGCATCGCCGTCGCAAAAACTGGCCGATGTACAGACGCTTTTGCATGAAGCGGGTGTGGCGGATAACGTGATTTGTTTTGAAGCGCAAATACCACTGGCGCTTTCTCGCGCAGCACTGCGTGCCAGAGTaga                                         >  1:137247/1‑140 (MQ=255)                                                                                             gCCTGATGGCATCGCCGTCGCAAAAACTGGCCGATGTACAGACGCTTTTGCATGAAGCGGGTGTGGCGGATAACGTGATTTGTTTTGAAGCGCAAATACCACTGGCGCTTTCTCGCGCAGCACTGCGTGCCAGAGTagaa                                        <  2:137247/140‑1 (MQ=255)                                                                                              ccTGATGGCATCGCCGTCGCAAAAACTGGCCGATGTACAGACGCTTTTGCATGAAGCGGGTGTGGCGGATAACGTGATTTGTTTTGAAGCGCAAATACCACTGGCGCTTTCTCGCGCAGCACTGCGTGCCAGAGTagaag                                       >  1:98278/1‑140 (MQ=255)                                                                                                tGATGGCATCGCCGTCGCAAAAACTGGCCGATGTACAGACGCTTTTGCATGAAGCGGGTGTGGCGGATAACGTGATTTGTTTTGAAGCGCAAATACCACTGGCGCTTTCTCGCGCAGCACTGCGTGCCAGAGTAGAagag                                     <  1:369689/140‑1 (MQ=255)                                                                                                   tGGCATCGCCGTCGCAAAAACTGGCCGATGTACAGACGCTTTTGCATGAAGCGGGTGTGGCGGATAACGTGATTTGTTTTGAAGCGCAAATACCACTGGCGCTTTCTCGCGCAGCACTGCGTGCCAGAGTAGAAGAGTGc                                  <  2:98278/140‑1 (MQ=255)                                                                                                       aTCGCCGTCGCAAAAACTGGCCGATGTACAGACGCTTTTGCATGAAGCGGGTGTGGCGGATAACGTGATTTGTTTTGAAGCGCAAATACCACTGGCGCTTTCTCGCGCAGCACTGCGTGCCAGAGTAGAAGAGTGCTGGc                              >  1:308948/1‑140 (MQ=255)                                                                                                        tCGCCGTCGCAAAAACTGGCCGATGTACAGACGCTTTTGCATGAAGCGGGTGTGGCGGATAACGTGATTTGTTTTGAAGCGCAAATACCACTGGCGCTTTCTCGCGCAGCACTGCGTGCCAGAGTAGAAGAGTGCTGGCa                             >  1:945463/1‑140 (MQ=255)                                                                                                         cGCCGTCGCAAAAACTGGCCGATGTACAGACGCTTTTGCATGAAGCGGGTGTGGCGGATAACGTGATTTGTTTTGAAGCGCAAATACCACTGGCGCTTTCTCGCGCAGCACTGCGTGCCAGAGTGGAAGAGTGCTGGCAt                            >  1:317468/1‑140 (MQ=255)                                                                                                            cGTCGCAAAAACTGGCCGATGTACAGACGCTTTTGCATGAAGCGGGTGTGGCGGATAACGTGATTTGTTTTGAAGCGCAAATACCACTGGCGCTTTCTCGCGCAGCACTGCGTGCCAGAGTAGAAGAGTGCTGGCATTTa                         >  1:92018/1‑140 (MQ=255)                                                                                                               cGCAAAAACTGGCCGATGTACAGACGCTTTTGCATGAAGCGGGTGTGGCGGATAACGTGATTTGTTTTGAAGCGCAAATACCACTGGCGCTTTCTCGCGCAGCACTGCGTGCCAGAGTAGAAGAGTGCTGGCATTTAACt                      <  2:945463/140‑1 (MQ=255)                                                                                                                                   cAGACGCTTTTGCATGAAGCGGGTGTGGCGGATAACGTGATTTGTTTTGAAGCGCAAATACCACTGGCGCTTTCTCGCGCAGCACTGCGTGCCAGAGTGGAAGAGTGCTGGCATTTAACTGAACAAAATGCCATGTACGa  <  2:317468/140‑1 (MQ=255)                                                                                                                                   cAGACGCTTTTGCATGAAGCGGGTGTGGCGGATAACGTGATTTGTTTTGAAGCGCAAATACCACTGGCGCTTTCTCGCGCAGCACTGCGTGCCAGAGTAGAAGAGTGCTGGCATTTAACTGAACAAAATGCCATGTACGa  <  2:625286/140‑1 (MQ=255)                                                                                                                                        |                                                                                                                                       GCTCCTGTTGCTCTCGGAAGGTTTAGATAAATCAACGCTGGCTGATGTCAAAAAGCAGTTGATCTGGCAAGGTTTTGGCGCACTGGCACCCAGCCTGATGGCATCGCCGTCGCAAAAACTGGCCGATGTACAGACACTTTTGCATGAAGCGGGTGTGGCGGATAACGTGATTTGTTTTGAAGCGCAAATACCACTGGCGCTTTCTCGCGCAGCACTGCGTGCCAGAGTAGAAGAGTGCTGGCATTTAACTGAACAAAATGCCATGTACGA  >  NC_000913/1463868‑1464137

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑