Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,575,399	T→C	T741A (ACG→GCG)	yddB ←	putative TonB‑dependent receptor

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,575,399	0	T	C	100.0%	201.6 / NA	56	T741A (ACG→GCG)	yddB	putative TonB‑dependent receptor
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (30/26);  total (30/26)

AGCCAGAAAGTACGGCCACTGGCGTAGGTCGCCACACCGGTGTTCGTTGTATCAACAGCGGTTTTGCTATCCAGTACATTGAGAATATCGGCACTGATCGTCAGGTTTTGTTGTTGCAGAAATTGTGGCGTCCAGGACAAGCGGGTGTCCCAGGTCAGGCTGCTATCCAGTTTTTCGTCAACATACTGCTTGTAATCTGAATATTCGCTGATGTATTGCGCATTTGTCTTACCAAGGATAATGCGAGCTTTACGCGCTTCTTGCC  >  NC_000913/1575269‑1575533                                                                                                                                   |                                                                                                                                       aGCCAGAAAGTACGGCCACTGGCGTAGGTCGCCACACCGGTGTTCGTTGTATCAACAGCGGTTTTGCTATCCAGTACATTGAGAATATCGGCACTGATCGTCAGGTTTTGTTGTTGCAGAAATTGTGGCGCCCAGGACaa                                                                                                                               >  2:597165/1‑140 (MQ=255)     aGAAAGTACGGCCACTGGCGTAGGTCGCCACACCGGTGTTCGTTGTATCAACAGCGGTTTTGCTATCCAGTACATTGAGAATATCGGCACTGATCGTCAGGTTTTGTTGTTGCAGAAATTGTGGCGCCCAGGACAAGCgg                                                                                                                           >  1:733803/1‑140 (MQ=255)      gAAAGTACGGCCACTGGCGTAGGTCGCCACACCGGTGTTCGTTGTATCAACAGCGGTTTTGCTATCCAGTACATTGAGAATATCGGCACTGATCGTCAGGTTTTGTTGTTGCAGAAATTGTGGCGCCCAGGACAAGCggg                                                                                                                          <  1:597165/140‑1 (MQ=255)      gAAAGTACGGCCACTGGCGTAGGTCGCCACACCGGTGTTCGTTGTATCAACAGCGGTTTTGCTATCCAGTACATTGAGAATATCGGCACTGATCGTCAGGTTTTGTTGTTGCAGAAATTGTGGCGCCCAGGACAAGCggg                                                                                                                          <  1:410726/140‑1 (MQ=255)             cGGCCACTGGCGTAGGTCGCCACACCGGTGTTCGTTGTATCAACAGCGGTTTTGCTATCCAGTACATTGAGAATATCGGCACTGATCGTCAGGTTTTGTTGTTGCAGAAATTGTGGCGCCCAGGACAAGCGGGTGTCCCa                                                                                                                   >  1:431885/1‑140 (MQ=255)                   cTGGCGTAGGTCGCCACACCGGTGTTCGTTGTATCAACAGCGGTTTTGCTATCCAGTACATTGAGAATATCGGCACTGATCGTCAGGTTTTGTTGTTGCAGAAATTGTGGCGCCCAGGACAAGCGGGTGTCCCAGGTCAg                                                                                                             >  1:806557/1‑140 (MQ=255)                     ggCGTAGGTCGCCACACCGGTGTTCGTTGTATCAACAGCGGTTTTGCTATCCAGTACATTGAGAATATCGGCACTGATCGTCAGGTTTTGTTGTTGCAGAAATTGTGGCGCCCAGGACAAGCGGGTGTCCCAGGTCAGgc                                                                                                           >  2:257018/1‑140 (MQ=255)                                ccACACCGGTGTTCGTTGTATCAACAGCGGTTTTGCTATCCAGTACATTGAGAATATCGGCACTGATCGTCAGGTTTTGTTGTTGCAGAAATTGTGGCGCCCAGGACAAGCGGGTGTCCCAGGTCAGGCTGCTATCCAGt                                                                                                >  1:331122/1‑140 (MQ=255)                                ccACACCGGTGTTCGTTGTATCAACAGCGGTTTTGCTATCCAGTACATTGAGAATATCGGCACTGATCGTCAGGTTTTGTTGTTGCAGAAATTGTGGCGCCCAGGACAAGCGGGTGTCCCAGGTCAGGCTGCTATCCAGt                                                                                                >  1:931912/1‑140 (MQ=255)                                    acCGGTGTTCGTTGTATCAACAGCGGTTTTGCTATCCAGTACATTGAGAATATCGGCACTGATCGTCAGGTTTTGTTGTTGCAGAAATTGTGGCGCCCAGGACAAGCGGGTGTCCCAGGTCAGGCTGCTATCCAGttttt                                                                                            <  1:325019/140‑1 (MQ=255)                                      cGGTGTTCGTTGTATCAACAGCGGTTTTGCTATCCAGTACATTGAGAATATCGGCACTGATCGTCAGGTTTTGTTGTTGCAGAAATTGTGGCGCCCAGGACAAGCGGGTGTCCCAGGTCAGGCTGCTATCCAGTTTTTCg                                                                                          >  2:399574/1‑140 (MQ=255)                                       ggTGTTCGTTGTATCAACAGCGGTTTTGCTATCCAGTACATTGAGAATATCGGCACTGATCGTCAGGTTTTGTTGTTGCAGAAATTGTGGCGCCCAGGACAAGCGGGTGTCCCAGGTCAGGCTGCTATCCAGTTTTTCGt                                                                                         <  2:431885/140‑1 (MQ=255)                                            tCGTTGTATCAACAGCGGTTTTGCTATCCAGTACATTGAGAATATCGGCACTGATCGTCAGGTTTTGTTGTTGCAGAAATTGTGGCGCCCAGGACAAGCGGGTGTCCCAGGTCAGGCTGCTATCCAGTTTTTCGTCAACa                                                                                    <  2:601786/140‑1 (MQ=255)                                               ttGTATCAACAGCGGTTTTGCTATCCAGTACATTGAGAATATCGGCACTGATCGTCAGGTTTTGTTGTTGCAGAAATTGTGGCGCCCAGGACAAGCGGGTGTCCCAGGTCAGGCTGCTATCCAGTTTTTCGTCAACATAc                                                                                 <  1:399574/140‑1 (MQ=255)                                                  tATCAACAGCGGTTTTGCTATCCAGTACATTGAGAATATCGGCACTGATCGTCAGGTTTTGTTGTTGCAGAAATTGTGGCGCCCAGGACAAGCGGGTGTCCCAGGTCAGGCTGCTATCCAGTTTTTCGTCAACATACTGc                                                                              <  2:733803/140‑1 (MQ=255)                                                   aTCAACAGCGGTTTTGCTATCCAGTACATTGAGAATATCGGCACTGATCGTCAGGTTTTGTTGTTGCAGAAATTGTGGCGCCCAGGACAAGCGGGTGTCCCAGGTCAGGCTGCTATCCAGTTTTTCGTCAACATACTGCt                                                                             >  1:149034/1‑140 (MQ=255)                                                       aCAGCGGTTTTGCTATCCAGTACATTGAGAATATCGGCACTGATCGTCAGGTTTTGTTGTTGCAGAAATTGTGGCGCCCAGGACAAGCGGGTGTCCCAGGTCAGGCTGCTATCCAGTTTTTCGt                                                                                         >  1:628304/1‑124 (MQ=255)                                                       aCAGCGGTTTTGCTATCCAGTACATTGAGAATATCGGCACTGATCGTCAGGTTTTGTTGTTGCAGAAATTGTGGCGCCCAGGACAAGCGGGTGTCCCAGGTCAGGCTGCTATCCAGTTTTTCGt                                                                                         <  2:628304/124‑1 (MQ=255)                                                        cAGCGGTTTTGCTATCCAGTACATTGAGAATATCGGCACTGATCGTCAGGTTTTGTTGTTGCAGAAATTGTGGCGCCCAGGACAAGCGGGTGTCCCAGGTCAGGCTGCTATCCAGTTTTTCGTCAACATACTGCTTGTaa                                                                        <  2:149034/140‑1 (MQ=255)                                                           cGGTTTTGCTATCCAGTACATTGAGAATATCGGCACTGATCGTCAGGTTTTGTTGTTGCAGAAATTGTGGCGCCCAGGACAAGCGGGTGTCCCAGGTCAGGCTGCTATCCAGTTTTTCGTCAACATACTGCTTGTAATCt                                                                     >  1:481124/1‑140 (MQ=255)                                                            ggTTTTGCTATCCAGTACATTGAGAATATCGGCACTGATCGTCAGGTTTTGTTGTTGCAGAAATTGTGGCGCCCAGGACAAGCGGGTGTCCCAGGTCAGGCTGCTATCCAGTTTTTCGTCAACa                                                                                    <  2:177216/124‑1 (MQ=255)                                                            ggTTTTGCTATCCAGTACATTGAGAATATCGGCACTGATCGTCAGGTTTTGTTGTTGCAGAAATTGTGGCGCCCAGGACAAGCGGGTGTCCCAGGTCAGGCTGCTATCCAGTTTTTCGTCAACa                                                                                    >  1:177216/1‑124 (MQ=255)                                                            ggTTTTGCTATCCAGTACATTGAGAATATCGGCACTGATCGTCAGGTTTTGTTGTTGCAGAAATTGTGGCGCCCAGGACAAGCGGGTGTCCCAGGTCAGGCTGCTATCCAGTTTTTCGTCAACATACTGCTTGt                                                                          >  1:223391/1‑134 (MQ=255)                                                            ggTTTTGCTATCCAGTACATTGAGAATATCGGCACTGATCGTCAGGTTTTGTTGTTGCAGAAATTGTGGCGCCCAGGACAAGCGGGTGTCCCAGGTCAGGCTGCTATCCAGTTTTTCGTCAACATACTGCTTGt                                                                          <  2:223391/134‑1 (MQ=255)                                                                  gCTATCCAGTACATTGAGAATATCGGCACTGATCGTCAGGTTTTGTTGTTGCAGAAATTGTGGCGCCCAGGACAAGCGGGTGTCCCAGGTCAGGCTGCTATCCAGTTTTTCGTCAACATACTGCTTGTAATCTGAATAtt                                                              <  2:778446/140‑1 (MQ=255)                                                                  gCTATCCAGTACATTGAGAATATCGGCACTGATCGTCAGGTTTTGTTGTTGCAGAAATTGTGGCGCCCAGGACAAGCGGGTGTCCCAGGTCAGGCTGCTATCCAGTTTTTCGTCAACATACTGCTTGTAATCTGAATAtt                                                              <  2:10480/140‑1 (MQ=255)                                                                    tATCCAGTACATTGAGAATATCGGCACTGATCGTCAGGTTTTGTTGTTGCAGAAATTGTGGCGCCCAGGACAAGCGGGTGTCCCAGGTCAGGCTGCTATCCAGTTTTTCGTCAACATACTGCTTGTAATCTGAATATTCg                                                            <  2:736665/140‑1 (MQ=255)                                                                     aTCCAGTACATTGAGAATATCGGCACTGATCGTCAGGTTTTGTTGTTGCAGAAATTGTGGCGCCCAGGACAAGCGGGTGTCCCAGGTCAGGCTGCTATCCAGTTTTTCGTCAACATACTGCTTGTAATCTGAATATTCGc                                                           <  1:257018/140‑1 (MQ=255)                                                                           tACATTGAGAATATCGGCACTGATCGTCAGGTTTTGTTGTTGCAGAAATTGTGGCGCCCAGGACAAGCGGGTGTCCCAGGTCAGGCTGCTATCCAGTTTTTCGTCAACATACTGCTTGTAATCTGAATAtt                                                              <  1:315728/131‑1 (MQ=255)                                                                           tACATTGAGAATATCGGCACTGATCGTCAGGTTTTGTTGTTGCAGAAATTGTGGCGCCCAGGACAAGCGGGTGTCCCAGGTCAGGCTGCTATCCAGTTTTTCGTCAACATACTGCTTGTAATCTGAATAtt                                                              >  2:315728/1‑131 (MQ=255)                                                                           tACATTGAGAATATCGGCACTGATCGTCAGGTTTTGTTGTTGCAGAAATTGTGGCGCCCAGGACAAGCGGGTGTCCCAGGTCAGGCTGCTATCCAGTTTTTCGTCAACATACTGCTTGTAATCTGAATATTCGCTGATGt                                                     >  2:244611/1‑140 (MQ=255)                                                                             cATTGAGAATATCGGCACTGATCGTCAGGTTTTGTTGTTGCAGAAATTGTGGCGCCCAGGACAAGCGGGTGTCCCAGGTCAGGCTGCTATCCAGTTTTTCGTCAACATACTGCTTGTAATCTGAATATTCGCTGATGTAt                                                   >  2:254445/1‑140 (MQ=255)                                                                                tGAGAATATCGGCACTGATCGTCAGGTTTTGTTGTTGCAGAAATTGTGGCGCCCAGGACAAGCGGGTGTCCCAGGTCAGGCTGCTATCCAGTTTTTCGTCAACATACTGCTTGTAATCTGAATATTCGCTGATGTATTgc                                                >  2:773899/1‑140 (MQ=255)                                                                                tGAGAATATCGGCACGGATCGTCAGGTTTTGTTGTTGCAGAAATTGTGGCGCCCAGGACAAGCGGGTGTCCCAGGTCAGGCTGCTATCCAGTTTTTCGTCAACATACTGCTTGTAATCTGAATATTCGCTg                                                         <  1:688408/131‑1 (MQ=255)                                                                                tGAGAATATCGGCACGGATCGTCAGGTTTTGTTGTTGCAGAAATTGTGGCGCCCAGGACAAGCGGGTGTCCCAGGTCAGGCTGCTATCCAGTTTTTCGTCAACATACTGCTTGTAATCTGAATATTCGCTg                                                         >  2:688408/1‑131 (MQ=255)                                                                                  agaATATCGGCACTGATCGTCAGGTTTTGTTGTTGCAGAAATTGTGGCGCCCAGGACAAGCGGGTGTCCCAGGTCAGGCTGCTATCCAGTTTTTCGTCAACATACTGCTTGTAATCTGAATATTCGCTGATGTATTgcgc                                              >  1:278144/1‑140 (MQ=255)                                                                                    aaTATCGGCACTGATCGTCAGGTTTTGTTGTTGCAGAAATTGTGGCGCCCAGGACAAGCGGGTGTCCCAGGTCAGGCTGCTATCCAGTTTTTCGTCAACATACTGCTTGTAATCTGAATATTCGCTGATGTATTGCGCAt                                            <  2:806557/140‑1 (MQ=255)                                                                                         cGGCACTGATCGTCAGGTTTTGTTGTTGCAGAAATTGTGGCGCCCAGGACAAGCGGGTGTCCCAGGTCAGGCTGCTATCCAGTTTTTCGTCAACATACTGCTTGTAATCTGAATATTCGCTGATGTATTGCGCATTTGTc                                       <  2:481124/140‑1 (MQ=255)                                                                                         cGGCACTGATCGTCAGGTTTTGTTGTTGCAGAAATTGTGGCGCCCAGGACAAGCGGGTGTCCCAGGTCAGGCTGCTATCCAGTTTTTCGTCAACATACTGCTTGTAATCTGAATATTCGCTGATGTATTGCGCATTTGTc                                       <  2:897148/140‑1 (MQ=255)                                                                                             aCTGATCGTCAGGTTTTGTTGTTGCAGAAATTGTGGCGCCCAGGACAAGCGGGTGTCCCAGGTCAGGCTGCTATCCAGTTTTTCGTCAACATACTGCTTGTAATCTGAATATTCGCTGATGTATTGCGCATTTGTCTTAc                                   <  1:773899/140‑1 (MQ=255)                                                                                                gATCGTCAGGTTTTGTTGTTGCAGAAATTGTGGCGCCCAGGACAAGCGGGTGTCCCAGGTCAGGCTGCTATCCAGTTTTTCGTCAACATACTGCTTGTAATCTGAATATTCGCTGATGTATTGCGCATTTGTCTTACCaa                                >  1:688908/1‑140 (MQ=255)                                                                                                 aTCGTCAGGTTTTGTTGTTGCAGAAATTGTGGCGCCCAGGACAAGCGGGTGTCCCAGGTCAGGCTGCTATCCAGTTTTTCGTCAACATACTGCTTGTAATCTGAATATTCGCTGATGTATTGCGCATTTGTCTTACCAAg                               >  1:939773/1‑140 (MQ=255)                                                                                                 aTCGTCAGGTTTTGTTGTTGCAGAAATTGTGGCGCCCAGGACAAGCGGGTGTCCCAGGTCAGGCTGCTATCCAGTTTTTCGTCAACATACTGCTTGTAATCTGAATATTCGCTGATGTATTGCGCATTTGTCTTACCAAg                               >  1:678720/1‑140 (MQ=255)                                                                                                 aTCGTCAGGTTTTGTTGTTGCAGAAATTGTGGCGCCCAGGACAAGCGGGTGTCCCAGGTCAGGCTGCTATCCAGTTTTTCGTCAACATACTGCTTGTAATCTGAATATTCGCTGATGTATTGCGCATTTGTCTTACCAAg                               >  1:445111/1‑140 (MQ=255)                                                                                                  tCGTAAGGTTTTGTTGTTGCAGAAATTGTGGCGCCCAGGACAAGCGGGTGTCCCAGGTCAGGCTGCTATCCAGTTTTTCGTCAACATACTGCTTGTAATCTGAATATTCGCTGATGTATTGCGCATTTGTCTTACCAAgg                              >  2:633798/1‑140 (MQ=255)                                                                                                      cAGGTTTTGTTGTTGCAGAAATTGTGGCGCCCAGGACAAGCGGGTGTCCCAGGTCAGGCTGCTATCCAGTTTTTCGTCAACATACTGCTTGTAATCTGAATATTCGCTGATGTATTGCGCATTTGTCTTACCAAGGATaa                          >  2:325925/1‑140 (MQ=255)                                                                                                      cAGGTTTTGTTGTTGCAGAAATTGTGGCGCCCAGGACAAGCGGGTGTCCCAGGTCAGGCTGCTATCCAGTTTTTCGTCAACATACTGCTTGTAATCTGAATATTCGCTGATGTATTGCGCATTTGTCTTACCAAGGATaa                          <  1:516083/140‑1 (MQ=255)                                                                                                         gTTTTGTTGTTGCAGAAATTGTGGCGCCCAGGACAAGCGGGTGTCCCAGGTCAGGCTGCTATCCAGTTTTTCGTCAACATACTGCTTGTAATCTGAATATTCGCTGATGTATTGCGCATTTGTCTTACCAAGGATAATGc                       >  1:34660/1‑140 (MQ=255)                                                                                                           tttGTTGTTGCAGAAATTGTGGCGCCCAGGACAAGCGGGTGTCCCAGGTCAGGCTGCTATCCAGTTTTTCGTCAACATACTGCTTGTAATCTGAATATTCGCTGATGTATTGCGCATTTGTCTTACCAAGGATAATGCGa                     >  1:503972/1‑140 (MQ=255)                                                                                                            ttgttgttgCAGAAATTGTGGCGCCCAGGACAAGCGGGTGTCCCAGGTCAGGCTGCTATCCAGTTTTTCGTCAACATACTGCTTGTAATCTGAATATTCGCTGATGTATTGCGCATTTGTCTTACCAAGGATAATGCGAg                    >  2:956389/1‑140 (MQ=255)                                                                                                             tgttgttgCAGAAATTGTGGCGCCCAGGACAAGCGGGTGTCCCAGGTCAGGCTGCTATCCAGTTTTTCGTCAACATACTGCTTGTAATCTGAATATTCGCTGATGTATTGCGCATTTGTCTTACCAAGGATAATGCGAGc                   >  2:592525/1‑140 (MQ=255)                                                                                                              gttgttgCAGAAATTGTGGCGCCCAGGACAAGCGGGTGTCCCAGGTCAGGCTGCTATCCAGTTTTTCGTCAACATACTGCTTGTAATCTGAATATTCGCTGATGTATTGCGCATTTGTCTTACCAAGGATAATGCGAGCt                  <  1:956389/140‑1 (MQ=255)                                                                                                              gttgttgCAGAAATTGTGGCGCCCAGGACAAGCGGGTGTCCCAGGTCAGGCTGCTATCCAGTTTTTCGTCAACATACTGCTTGTAATCTGAATATTCGCTGATGTATTGCGCATTTGTCTTACCAAGGATAATGCGAGCt                  <  1:592525/140‑1 (MQ=255)                                                                                                                   tgCAGAAATTGTGGCGCCCAGGACAAGCGGGTGTCCCAGGTCAGGCTGCTATCCAGTTTTTCGTCAACATACTGCTTGTAATCTGAATATTCGCTGATGTATTGCGCATTTGTCTTACCAAGGATAATGCGAGCTTTAcg             <  1:244611/140‑1 (MQ=255)                                                                                                                        aaaTTGTGGCGCCCAGGACAAGCGGGTGTCCCAGGTCAGGCTGCTATCCAGTTTTTCGTCAACATACTGCTTGTAATCTGAATATTCGCTGATGTATTGCGCATTTGTCTTACCAAGGATAATGCGAGCTTTACGCGctt        <  2:503972/140‑1 (MQ=255)                                                                                                                              tgGCGCCCAGGACAAGCGGGTGTCCCAGGTCAGGCTGCTATCCAGTTTTTCGTCAACATACTGCTTGTAATCTGAATATTCGCTGATGTATTGCGCATTTGTCTTACCAAGGATAATGCGAGCTTTACGCGCTTCTTgcc  >  1:632006/1‑140 (MQ=255)                                                                                                                                   |                                                                                                                                       AGCCAGAAAGTACGGCCACTGGCGTAGGTCGCCACACCGGTGTTCGTTGTATCAACAGCGGTTTTGCTATCCAGTACATTGAGAATATCGGCACTGATCGTCAGGTTTTGTTGTTGCAGAAATTGTGGCGTCCAGGACAAGCGGGTGTCCCAGGTCAGGCTGCTATCCAGTTTTTCGTCAACATACTGCTTGTAATCTGAATATTCGCTGATGTATTGCGCATTTGTCTTACCAAGGATAATGCGAGCTTTACGCGCTTCTTGCC  >  NC_000913/1575269‑1575533

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑