Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,598,771	C→T	R480H (CGC→CAC)	lsrK ←	autoinducer‑2 kinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,598,771	0	C	T	100.0%	220.7 / NA	64	R480H (CGC→CAC)	lsrK	autoinducer‑2 kinase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (34/30);  total (34/30)

CGTCGTCAGTCCATGATCAACCAGCCCCAGCTGATCCTGATAAACTGCCTGCCACTTATCGCGTGAATCCTGATAAAGTTCATGCTTTTCCGGGTCTGGTGTGTGCGTCCGTTCCCAGCGAACCAGGCGTTCTCCGGTTTCTGCCATTGATGAAAAAATTCCGGCACCGACGCCAGCTGCAATGGCACATCCTAATGCAGTGGCTTCTTTGACCACCGGAATATTGACGGGTAATCCCGAGACATCAG  >  NC_000913/1598644‑1598891                                                                                                                                |                                                                                                                         cgtcgtCAGTCCATGATCAACCAGCCCCAGCTGATCCTGATAAACTGCCTGCCACTTATCGCGTGAATCCTGATAAAGTTCATGCTTTTCCGGGTCTGGTGTGTGCGTCCGTTCCCAGCGAACCAGGTGTTCTCCGGttt                                                                                                              >  2:638857/1‑140 (MQ=255) cgtcgtCAGTCCATGATCAACCAGCCCCAGCTGATCCTGATAAACTGCCTGCCACTTATCGCGTGAATCCTGATAAAGTTCATGCTTTTCCGGGTCTGGTGTGTGCGTCCGTTCCCAGCGAACCAGGTGTTCTCCGGttt                                                                                                              >  2:621608/1‑140 (MQ=255) cgtcgtCAGTCCATGATCAACCAGCCCCAGCTGATCCTGATAAACTGCCTGCCACTTATCGCGTGAATCCTGATAAAGTTCATGCTTTTCCGGGTCTGGTGTGTGCGTCCGTTCCCAGCGAACCAGGTGTTCTCCGGttt                                                                                                              >  2:179266/1‑140 (MQ=255) cgtcgtCAGTCCATGATCAACCAGCCCCAGCTGATCCTGATAAACTGCCTGCCACTTATCGCGTGAATCCTGATAAAGTTCATGCTTTTCCGGGTCTGGTGTGTGCGTCCGTTCCCAGCGAACCAGGTGTTCTCCGGttt                                                                                                              >  2:181689/1‑140 (MQ=255) cgtcgtCAGTCCATGATCAACCAGCCCCAGCTGATCCTGATAAACTGCCTGCCACTTATCGCGTGAATCCTGATAAAGTTCATGCTTTTCCGGGTCTGGTGTGTGCGTCCGTTCCCAGCGAACCAGGTGTTCTCCGGttt                                                                                                              >  2:311907/1‑140 (MQ=255) cgtcgtCAGTCCATGATCAACCAGCCCCAGCTGATCCTGATAAACTGCCTGCCACTTATCGCGTGAATCCTGAT‑AAGTTCATGCTTTTCCGGGTCTGGTGTGTGCGTCCGTTCCCAGCGAACCAGGTGTTCTCCGGTTTc                                                                                                             >  2:128667/1‑140 (MQ=255)           ccATGATCAACCAGCCCCAGCTGATCCTGATAAACTGCCTGCCACTTATCGCGTGAATCCTGATAAAGTTCATGCTTTTCCGGGTCTGGTGTGTGCGTCCGTTCCCAGCGAACCAGGTGTTCTCCGGTTTCTGCCATtga                                                                                                    <  1:179266/140‑1 (MQ=255)             aTGATCAACCAGCCCCAGCTGATCCTGATAAACTGCCTGCCACTTATCGCGTGAATCCTGATAAAGTTCATGCTTTTCCGGGTCTGGTGTGTGCGTCCGTTCCCAGCGAACCAGGTGTTCTCCGGTTTCTGCCATtgatg                                                                                                  >  1:587808/1‑140 (MQ=255)                    aCCAGCCCCAGCTGATCCTGATAAACTGCCTGCCACTTATCGCGTGAATCCTGATAAAGTTCATGCTTTTCCGGGTCTGGTGTGTGCGTCCGTTCCCAGCGAACCAGGTGTTCTCCGGTTTCTGCCa                                                                                                        >  1:365982/1‑127 (MQ=255)                    aCCAGCCCCAGCTGATCCTGATAAACTGCCTGCCACTTATCGCGTGAATCCTGATAAAGTTCATGCTTTTCCGGGTCTGGTGTGTGCGTCCGTTCCCAGCGAACCAGGTGTTCTCCGGTTTCTGCCa                                                                                                        <  2:365982/127‑1 (MQ=255)                    aCCAGCCCCAGCTGATCCTGATAAACTGCCTGCCACTTATCGCGTGAATCCTGATAAAGTTCATGCTTTTCCGGGTCTGGTGTGTGCGTCCGTTCCCAGCGAACCAGGTGTTCTCCGGTTTCTGCCATTGATGAAAAAAt                                                                                           >  2:96021/1‑140 (MQ=255)                    aCCAGCCCCAGCTGATCCTGATAAACTGCCTGCCACTTATCGCGTGAATCCTGATAAAGTTCATGCTTTTCCGGGTCTGGTGTGTGCGTCCGTTCCCAGCGAACCAGGTGTTCTCCGGTTTCTGCCATTGATGAAAAAAt                                                                                           >  1:753484/1‑140 (MQ=255)                    aCCAGCCCCAGCTGATCCTGATAAACTGCCTGCCACTTATCGCGTGAATCCTGATAAAGTTCATGCTTTTCCGGGTCTGGTGTGTGCGTCCGTTCCCAGCGAACCAGGTGTTCTCCGGTTTCTGCCATTGATGAAAAAAt                                                                                           >  2:500193/1‑140 (MQ=255)                    aCCAGCCCCAGCTGATCCTGATAAACTGCCTGCCACTTATCGCGTGAATCCTGATAAAGTTCATGCTTTTCCGGGTCTGGTGTGTGCGTCCGTTCCCAGCGAACCAGGTGTTCTCCGGTTTCTGCCATTGATGAAAAAAt                                                                                           >  1:133756/1‑140 (MQ=255)                         ccccAGCTGATCCTGATAAACTGCCTGCCACTTATCGCGTGAATCCTGATAAAGTTCATGCTTTTCCGGGTCTGGTGTGTGCGTCCGTTCCCAGCGAACCAGGTGTTCTCCGGTTTCTGCCATTGATGaaa                                                                                               >  1:270793/1‑131 (MQ=255)                         ccccAGCTGATCCTGATAAACTGCCTGCCACTTATCGCGTGAATCCTGATAAAGTTCATGCTTTTCCGGGTCTGGTGTGTGCGTCCGTTCCCAGCGAACCAGGTGTTCTCCGGTTTCTGCCATTGATGaaa                                                                                               <  2:270793/131‑1 (MQ=255)                         ccccAGCTGATCCTGATAAACTGCCTGCCACTTATCGCGTGAATCCTGATAAAGTTCATGCTTTTCCGGGTCTGGTGTGTGCGTCCGTTCCCAGCGAACCAGGTGTTCTCCGGTTTCTGCCATTGATGAAAAAATTc                                                                                         <  2:454222/137‑1 (MQ=255)                         ccccAGCTGATCCTGATAAACTGCCTGCCACTTATCGCGTGAATCCTGATAAAGTTCATGCTTTTCCGGGTCTGGTGTGTGCGTCCGTTCCCAGCGAACCAGGTGTTCTCCGGTTTCTGCCATTGATGAAAAAATTc                                                                                         >  1:454222/1‑137 (MQ=255)                         ccccAGCTGATCCTGATAAACTGCCTGCCACTTATCGCGTGAATCCTGATAAAGTTCATGCTTTTCCGGGTCTGGTGTGTGCGTCCGTTCCCAGCGAACCAGGTGTTCTCCGGTTTCTGCCATTGATGAAAAAATTCCgg                                                                                      >  1:479005/1‑140 (MQ=255)                             aGCTGATCCTGATAAACTGCCTGCCACTTATCGCGTGAATCCTGATAAAGTTCATGCTTTTCCGGGTCTGGTGTGTGCGTCCGTTCCCAGCGAACCAGGTGTTCTCCGGTTTCTGCCATTGATGAAAAAATTCCGGCAcc                                                                                  >  2:469924/1‑140 (MQ=255)                                   tCCTGATAAACTGCCTGCCACTTATCGCGTGAATCCTGATAAAGTTCATGCTTTTCCGGGTCTGGTGTGTGCGTCCGTTCCCAGCGAACCAGGTGTTCTCCGGTTTCTGCCATTGATGAAAAAATTCCGGCACCGACGcc                                                                            >  1:202023/1‑140 (MQ=255)                                   tCCTGATAAACTGCCTGCCACTTATCGCGTGAATCCTGATAAAGTTCATGCTTTTCCGGGTCTGGTGTGTGCGTCCGTTCCCAGCGAACCAGGTGTTCTCCGGTTTCTGCCATTGATGAAAAAATTCCGGCACCGACGcc                                                                            >  2:16967/1‑140 (MQ=255)                                   tCCTGATAAACTGCCTGCCACTTATCGCGTGAATCCTGATAAAGTTCATGCTTTTCCGGGTCTGGTGTGTGCGTCCGTTCCCAGCGAACCAGGTGTTCTCCGGTTTCTGCCATTGATGAAAAAATTCCGGCACCGACGcc                                                                            >  2:831802/1‑140 (MQ=255)                                   tCCTGATAAACTGCCTGCCACTTATCGCGTGAATCCTGATAAAGTTCATGCTTTTCCGGGTCTGGTGTGTGCGTCCGTTCCCAGCGAACCAGGTGTTCTCCGGTTTCTGCCATTGATGAAAAAATTCCGGCACCGACGcc                                                                            >  1:747810/1‑140 (MQ=255)                                      tGATAAACTGCCTGCCACTTATCGCGTGAATCCTGATAAAGTTCATGCTTTTCCGGGTCTGGTGTGTGCGTCCGTTCCCAGCGAACCAGGTGTTCTCCGGTTTCTGCCATTGATGAAAAAATTCCGGCACCGACGCCAGc                                                                         <  1:888015/140‑1 (MQ=255)                                       gATAAACTGCCTGCCACTTATCGCGTGAATCCTGATAAAGTTCATGCTTTTCCGGGTCTGGTGTGTGCGTCCGTTCCCAGCGAACCAGGTGTTCTCCGGTTTCTGCCATTGATGAAAAAATTCCGGCACCGACGCCAGCt                                                                        >  2:224802/1‑140 (MQ=255)                                        aTAAACTGCCTGCCACTTATCGCGTGAATCCTGATAAAGTTCATGCTTTTCCGGGTCTGGTGTGTGCGTCCGTTCCCAGCGAACCAGGTGTTCTCCGGTTTCTGCCATTGATGAAAAAATTCCGGCACCGACGCCAGCTg                                                                       >  1:541853/1‑140 (MQ=255)                                        aTAAACTGCCTGCCACTTATCGCGTGAATCCTGATAAAGTTCATGCTTTTCCGGGTCTGGTGTGTGCGTCCGTTCCCAGCGAACCAGGTGTTCTCCGGTTTCTGCCATTGATGAAAAAATTCCGGCACCGACGCCAGCTg                                                                       >  1:449774/1‑140 (MQ=255)                                          aaaCTGCCTGCCACTTATCGCGTGAATCCTGATAAAGTTCATGCTTTTCCGGGTCTGGTGTGTGCGTCCGTTCCCAGCGAACCAGGTGTTCTCCGGTTTCTGCCATTGATGAAAAAATTCCGGCACCGACGCCAGCTGCa                                                                     <  1:224802/140‑1 (MQ=255)                                           aaCTGCCTGCCACTTATCGCGTGAATCCTGATAAAGTTCATGCTTTTCCGGGTCTGGTGTGTGCGTCCGTTCCCAGCGAACCAGGTGTTCTCCGGTTTCTGCCATTGATGAAAAAATTCCGGCACCGACGCCAGCTGCaa                                                                    <  2:587808/140‑1 (MQ=255)                                              tgcctgccACTTATCGCGTGAATCCTGATAAAGTTCATGCTTTTCCGGGTCTGGTGTGTGCGTCCGTTCCCAGCGAACCAGGTGTTCTCCGGTTTCTGCCATTGATGAAAAAATTCCGGCACCGACGCCAGCTGCAATgg                                                                 <  2:114111/140‑1 (MQ=255)                                                cctgccACTTATCGCGTGAATCCTGATAAAGTTCATGCTTTTCCGGGTCTGGTGTGTGCGTCCGTTCCCAGCGAACCAGGTGTTCTCCGGTTTCTGCCATTGATGAAAAAATTCTGGCACCGACGCCAGCTGCAATGGca                                                               <  2:772495/140‑1 (MQ=255)                                                cctgccACTTATCGCGTGAATCCTGATAAAGTTCATGCTTTTCCGGGTCTGGTGTGTGCGTCCGTTCCCAGCGAACCAGGTGTTCTCCGGTTTCTGCCATTGATGAAAAAATTCCGGCACCGACGCCAGCTGCAATGGca                                                               <  1:500193/140‑1 (MQ=255)                                                cctgccACTTATCGCGTGAATCCTGATAAAGTTCATGCTTTTCCGGGTCTGGTGTGTGCGTCCGTTCCCAGCGAACCAGGTGTTCTCCGGTTTCTGCCATTGATGAAAAAATTCCGGCACCGACGCCAGCTGCAATGGca                                                               <  1:606830/140‑1 (MQ=255)                                                cctgccACTTATCGCGTGAATCCTGATAAAGTTCATGCTTTTCCGGGTCTGGTGTGTGCGTCCGTTCCCAGCGAACCAGGTGTTCTCCGGTTTCTGCCATTGATGAAAAAATTCCGGCACCGACGCCAGCTGCAATGGca                                                               <  1:96021/140‑1 (MQ=255)                                                cctgccACTTATCGCGTGAATCCTGATAAAGTTCATGCTTTTCCGGGTCTGGTGTGTGCGTCCGTTCCCAGCGAACCAGGTGTTCTCCGGTTTCTGCCATTGATGAAAAAATTACGGCACCGACGCCAGCTGCAATGGca                                                               <  1:218320/140‑1 (MQ=255)                                                    ccACTTATCGCGTGAATCCTGATAAAGTTCATGCTTTTCCGGGTCTGGTGTGTGCGTCCGTTCCCAGCGAACCAGGTGTTCTCCGGTTTCTGCCATTGATGAAAAAATTCCGGCACCGACGCCAGCTGCAATGGCACATc                                                           >  2:265553/1‑140 (MQ=255)                                                     cACTTATCGCGTGAATCCTGATAAAGTTCATGCTTTTCCGGGTCTGGTGTGTGCGTCCGTTCCCAGCGAACCAGGTGTTCTCCGGTTTCTGCCATTGATGAAAAAATTCCGGCACCGACGCCAGCTGCAATGGCACATcc                                                          <  1:469924/140‑1 (MQ=255)                                                      aCTTATCGCGTGAATCCTGATAAAGTTCATGCTTTTCCGGGTCTGGTGTGTGCGTCCGTTCCCAGCGAACCAGGTGTTCTCCGGTTTCTGCCATTGATGAAAAAATTCCGGCACCGACGCCAGCTGCAATGGCACATCCt                                                         <  2:449774/140‑1 (MQ=255)                                                          aTCGCGTGAATCCTGATAAAGTTCATGCTTTTCCGGGTCTGGTGTGTGCGTCCGTTCCCAGCGAACCAGGTGTTCTCCGGTTTCTGCCATTGATGAAAAAATTCCGGCACCGACGCCAGCTGCAATGGCACATCCTAATg                                                     >  2:423602/1‑140 (MQ=255)                                                             gcgTGAATCCTGATAAAGTTCATGCTTTTCCGGGTCTGGTGTGTGCGTCCGTTCCCAGCGAACCAGGTGTTCTCCGGTTTCTGCCATTGATGAAAAAATTCCGGCACCGACGCCAGCTGCAATGGCACATCCTAATGCAg                                                  <  1:16967/140‑1 (MQ=255)                                                               gTGAATCCTGATAAAGTTCATGCTTTTCCGGGTCTGGTGTGTGCGTCCGTTCCCAGCGAACCAGGTGTTCTCCGGTTTCTGCCATTGATGAAAAAATTCCGGCACCGACGCCAGCTGCAATGGCACATCCTAATGCAGTg                                                <  2:669263/140‑1 (MQ=255)                                                                tGAATCCTGATAAAGTTCATGCTTTTCCGGGTCTGGTGTGTGCGTCCGTTCCCAGCGAACCAGGTGTTCTCCGGTTTCTGCCATTGATGAAAAAATTCCGGCACCGACGCCAGCTGCAATGGCACATCCTAATGCAGTgg                                               <  1:265553/140‑1 (MQ=255)                                                                  aaTCCTGATAAAGTTCATGCTTTTCCGGGTCTGGTGTGTGCGTCCGTTCCCAGCGAACCAGGTGTTCTCCGGTTTCTGCCATTGATGAAAAAATTCCGGCACCGACGCCAGCTGCAATGGCACATCCTAATGCAGTgg                                               >  2:51007/1‑138 (MQ=255)                                                                  aaTCCTGATAAAGTTCATGCTTTTCCGGGTCTGGTGTGTGCGTCCGTTCCCAGCGAACCAGGTGTTCTCCGGTTTCTGCCATTGATGAAAAAATTCCGGCACCGACGCCAGCTGCAATGGCACATCCTAATGCAGTgg                                               <  1:51007/138‑1 (MQ=255)                                                                  aaTCCTGATAAAGTTCATGCTTTTCCGGGTCTGGTGTGTGCGTCCGTTCCCAGCGAACCAGGTGTTCTCCGGTTTCTGCCATTGATGAAAAAATTCCGGCACCGACGCCAGCTGCAATGGCACATCCTAATGCAGTGGct                                             >  1:147559/1‑140 (MQ=255)                                                                    tCCTGATAAAGTTCATGCTTTTCCGGGTCTGGTGTGTGCGTCCGTTCCCAGCGAACCAGGTGTTCTCCGGTTTCTGCCATTGATGAAAAAATTCCGGCACCGACGCCAGCTGCAATGGCACATCCTAATGCAGTgg                                               >  2:443855/1‑136 (MQ=255)                                                                    tCCTGATAAAGTTCATGCTTTTCCGGGTCTGGTGTGTGCGTCCGTTCCCAGCGAACCAGGTGTTCTCCGGTTTCTGCCATTGATGAAAAAATTCCGGCACCGACGCCAGCTGCAATGGCACATCCTAATGCAGTgg                                               <  1:443855/136‑1 (MQ=255)                                                                        gATAAAGTTCATGCTTTTCCGGGTCTGGTGTGTGCGTCCGTTCCCAGCGAACCAGGTGTTCTCCGGTTTCTGCCATTGATGAAAAAATTCCGGCACCGACGCCAGCTGCAATGGCACATCCTAATGCAGTGGCTTCTTTg                                       <  2:753484/140‑1 (MQ=255)                                                                          tAAAGTTCATGCTTTTCCGGGTCTGGTGTGTGCGTCCGTTCCAAGCGAACCAGGTGTTCTCCGGTTTCTGCCATTGATGAAAAAATTCCGGCACCGACGCCAGCTGCAATGGCACATCCTAATGCAGTGGCTTCTTTGac                                     <  2:905657/140‑1 (MQ=255)                                                                             aGTTCATGCTTTTCCGGGTCTGGTGTGTGCGTCCGTTCCCAGCGAACCAGGTGTTCTCCGGTTTCTGCCATTGATGAAAAAATTCCGGCACCGACGCCAGCTGCAATGGCACATCCTAATGCAGTGGCTTCTTTGaccac                                  <  1:128667/140‑1 (MQ=255)                                                                             aGTTCATGCTTTTCCGGGTCTGGTGTGTGCGTCCGTTCCCAGCGAACCAGGTGTTCTCCGGTTTCTGCCATTGATGAAAAAATTCCGGCACCGACGCCAGCTGCAATGGCACATCCTAATGCAGTGGCTTCTTTGaccac                                  <  1:311907/140‑1 (MQ=255)                                                                             aGTTCATGCTTTTCCGGGTCTGGTGTGTGCGTCCGTTCCCAGCGAACCAGGTGTTCTCCGGTTTCTGCCATTGATGAAAAAATTCCGGCACCGACGCCAGCTGCAATGGCACATCCTAATGCAGTGGCTTCTTTGaccac                                  <  1:181689/140‑1 (MQ=255)                                                                              gTTCATGCTTTTCCGGGTCTGGTGTGTGCGTCCGTTCCCAGCGAACCAGGTGTTCTCCGGTTTCTGCCATTGATGAAAAAATTCCGGCACCGACGCCAGCTGCAATGGCACATCCTAATGCAGTGGCTTCTTTGaccacc                                 >  1:65380/1‑140 (MQ=255)                                                                               ttCATGCTTTTCCGGGTCTGGTGTGTGCGTCCGTTCCCAGCGAACCAGGTGTTCTCCGGTTTCTGCCATTGATGAAAAAATTCCGGCACCGACGCCAGCTGCAATGGCACATCCTAATGCAGTGGCTTCTTTGACCACCg                                >  1:748498/1‑140 (MQ=255)                                                                                 cATGCTTTTCCGGGTCTGGTGTGTGCGTCCGTTCCCAGCGAACCAGGTGTTCTCCGGTTTCTGCCATTGATGAAAAAATTCCGGCACCGACGCCAGCTGCAATGGCACATCCTAATGCAGTGGCTTCTTTGACCACCGGa                              <  1:417719/140‑1 (MQ=255)                                                                                 cATGCTTTTCCGGGTCTGGTGTGTGCGTCCGTTCCCAGCGAACCAGGTGTTCTCCGGTTTCTGCCATTGATGAAAAAATTCCGGCACCGACGCCAGCTGCAATGGCACATCCTAATGCAGTGGCTTCTTTGACCACCGGa                              >  1:357704/1‑140 (MQ=255)                                                                                      ttttCCGGGTCTGGTGTGTGCGTCCGTTCCCAGCGAACCAGGTGTTCTCCGGTTTCTGCCATTGATGAAAAAATTCCGGCACCGACGCCAGCTGCAATGGCACATCCTAATGCAGTGGCTTCTTTGACCACCGGAATAtt                         <  2:747810/140‑1 (MQ=255)                                                                                       tttCCGGGTCTGGTGTGTGCGTCCGTTCCCAGCGAACCAGGTGTTCTCCGGTTTCTGCCATTGATGAAAAAATTCCGGCACCGACGCCAGCTGCAATGGCACATCCTAATGCAGTGGCTTCTTTGACCACCGGAATATTg                        <  2:133756/140‑1 (MQ=255)                                                                                           cGGGTCTGGTGTGTGCGTCCGTTCCCAGCGAACCAGGTGTTCTCCGGTTTCTGCCATTGATGAAAAAATTCCGGCACCGACGCCAGCTGCAATGGCACATCCTAATGCAGTGGCTTCTTTGACCACCGGAATATTGACgg                    <  2:479005/140‑1 (MQ=255)                                                                                                cTGGTGTGTGCGTCCGTTCCCAGCGAACCAGGTGTTCTCCGGTTTCTGCCATTGATGAAAAAATTCCGGCACCGACGCCAGCTGCAATGGCACATCCTAATGCAGTGGCTTCTTTGACCACCGGAATATTGACGGGTAAt               <  2:541853/140‑1 (MQ=255)                                                                                                        tgCGTCCGTTCCCAGCGAACCAGGTGTTCTCCGGTTTCTGCCATTGATGAAAAAATTCCGGCACCGACGCCAGCTGCAATGGCACATCCTAATGCAGTGGCTTCTTTGACCACCGGAATATTGACGGGTAATCCCGAGAc       >  1:411789/1‑140 (MQ=255)                                                                                                            tCCGTTCCCAGCGAACCAGGTGTTCTCCGGTTTCTGCCATTGATGAAAAAATTCCGGCACCGACGCCAGCTGCAATGGCACATCCTAATGCAGTGGCTTCTTTGACCACCGGAATATTGACGGGTAATCCCGAGACATCa   >  1:236740/1‑140 (MQ=255)                                                                                                             ccGTTCCCAGCGAACCAGGTGTTCTCCGGTTTCTGCCATTGATGAAAAAATTCCGGCACCGACGCCAGCTGCAATGGCACATCCTAATGCAGTGGCTTCTTTGACCACCGGAATATTGACGGGTAATCCCGAGACATCAg  >  1:123120/1‑140 (MQ=255)                                                                                                                                |                                                                                                                         CGTCGTCAGTCCATGATCAACCAGCCCCAGCTGATCCTGATAAACTGCCTGCCACTTATCGCGTGAATCCTGATAAAGTTCATGCTTTTCCGGGTCTGGTGTGTGCGTCCGTTCCCAGCGAACCAGGCGTTCTCCGGTTTCTGCCATTGATGAAAAAATTCCGGCACCGACGCCAGCTGCAATGGCACATCCTAATGCAGTGGCTTCTTTGACCACCGGAATATTGACGGGTAATCCCGAGACATCAG  >  NC_000913/1598644‑1598891

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑