Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,600,066	T→C	V48V (GTA→GTG)	lsrK ←	autoinducer‑2 kinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,600,066	0	T	C	100.0%	150.0 / NA	44	V48V (GTA→GTG)	lsrK	autoinducer‑2 kinase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (21/23);  total (21/23)

CACGCATCGAACATGCCGAAACGGCAGCGATATACTCCGGGGCTATGCCGGCGTTGTGCAGCGCCTGGCGCATACACTCACACGCCAGTTGCCAGTTTTTGTTGAGATCAAATTCCATAGAACCAGGAACGTCCGGTACTGCCAGATGCCGCCACTCCGCCTGTCCCACTGCTATTTGATTGCCTTCCAGGTCGAATATCACAGCCCGAATACTTCCGGTGCCTGCATCCAGCGCCATCAGGTAGTACTTTGATTCTGAAAGGGTAAAGAG  >  NC_000913/1599930‑1600200                                                                                                                                         |                                                                                                                                       cACGCATCGAACATGCCGAAACGGCAGCGATATACTCCGGGGCTATGCCGGCGTTGTGCAGCGCCTGGCGCATACACTCACACGCCAGTTGCCAGTTTTTGTTGAGATCAAATTCCATAGAACCAGGAACGTCCGGCACt                                                                                                                                     >  2:952738/1‑140 (MQ=255)  aCGCATCGAACATGCCGAAACGGCAGCGATATACTCCGGGGCTATGCCGGCGTTGTGCAGCGCCTGGCGCATACACTCACACGCCAGTTGCCAGTTTTTGTTGAGATCAAATTCCATAGAACCAGGAACGTCCGGCACTg                                                                                                                                    <  1:558832/140‑1 (MQ=255)       tCGAACATGCCGAAACGGCAGCGATATACTCCGGGGCTATGCCGGCGTTGTGCAGCGCCTGGCGCATACACTCACACGCCAGTTGCCAGTTTTTGTTGAGATCAAATTCCATAGAACCAGGAACGTCCGGCACTGCCAGa                                                                                                                               <  1:319431/140‑1 (MQ=255)           aCATGCCGAAACGGCAGCGATATACTCCGGGGCTATGCCGGCGTTGTGCAGCGCCTGGCGCATACACTCACACGCCAGTTGCCAGTTTTTGTTGAGATCAAATTCCATAGAACCAGGAACGTCCGGCACTGCCAGATgcc                                                                                                                           <  1:853448/140‑1 (MQ=255)               gCCGAAACGGCAGCGATATACTCCGGGGCTATGCCGGCGTTGTGCAGCGCCTGGCGCATACACTCACACGCCAGTTGCCAGTTTTTGTTGAGATCAAATTCCATAGAACCAGGAACGTCCGGCACTGCCAGATGCCGCCa                                                                                                                       >  1:278244/1‑140 (MQ=255)                ccGAAACGGCAGCGATATACTCCGGGGCTATGCCGGCGTTGTGCAGCGCCTGGCGCATACACTCACACGCCAGTTGCCAGTTTTTGTTGAGATCAAATTCCATAGAACCAGGAACGTCCGGCACTGCCAGATGCCGCCAc                                                                                                                      >  1:494655/1‑140 (MQ=255)                ccGAAACGGCAGCGATATACTCCGGGGCTATGCCGGCGTTGTGCAGCGCCTGGCGCATACACTCACACGCCAGTTGCCAGTTTTTGTTGAGATCAAATTCCATAGAACCAGGAACGTCCGGCACTGCCAGATGCCGCCAc                                                                                                                      >  2:936859/1‑140 (MQ=255)                 cGAAACGGCAGCGATATACTCCGGGGCTATGCCGGCGTTGTGCAGCGCCTGGCGCATACACTCACACGCCAGTTGCCAGTTTTTGTTGAGATCAAATTCCATAGAACCAGGAACGTCCGGCACTGCCAGATGCCGCCACt                                                                                                                     <  1:401224/140‑1 (MQ=255)                     aCGGCAGCGATATACTCCGGGGCTATGCCGGCGTTGTGCAGCGCCTGGCGCATACACTCACACGCCAGTTGCCAGTTTTTGTTGAGATCAAATTCCATAGAACCAGGAACGTCCGGCACTGCCAGATGCCGCCACTCCGc                                                                                                                 <  1:641342/140‑1 (MQ=255)                      cGGCAGCGATATACTCCGGGGCTATGCCGGCGTTGTGCAGCGCCTGGCGCATACACTCACACGCCAGTTGCCAGTTTTTGTTGAGATCAAATTCCATAGAACCAGGAACGTCCGGCACTGCCAGATGCCGCCACTCCGcc                                                                                                                <  1:952738/140‑1 (MQ=255)                         cAGCGATATACTCCGGGGCTATGCCGGCGTTGTGCAGCGCCTGGCGCATACACTCACACGCCAGTTGCCAGTTTTTGTTGAGATCAAATTCCATAGAACCAGGAACGTCCGGCACTGCCAGATGCCGCCACTCCGCCTGt                                                                                                             >  2:500444/1‑140 (MQ=255)                                     ccGGGGCTATGCCGGCGTTGTGCAGCGCCTGGCGCATACACTCACACGCCAGTTGCCAGTTTTTGTTGAGATCAAATTCCATAGAACCAGGAACGTCCGGCACTGCCAGATGCCGCCACTCCGCCTGTCCCACTGCTAtt                                                                                                 <  1:650535/140‑1 (MQ=255)                                      cGGGGCTATGCCGGCGTTGTGCAGCGCCTGGCGCATACACTCACACGCCAGTTGCCAGTTTTTGTTGAGATCAAATTCCATAGAACCAGGAACGTCCGGCACTGCCAGATGCCGCCACTCCGCCTGTcc                                                                                                           >  2:301926/1‑129 (MQ=255)                                      cGGGGCTATGCCGGCGTTGTGCAGCGCCTGGCGCATACACTCACACGCCAGTTGCCAGTTTTTGTTGAGATCAAATTCCATAGAACCAGGAACGTCCGGCACTGCCAGATGCCGCCACTCCGCCTGTcc                                                                                                           <  1:301926/129‑1 (MQ=255)                                        gggCTATGCCGGCGTTGTGCAGCGCCTGGCGCATACACTCACACGCCAGTTGCCAGTTTTTGTTGAGATCAAATTCCATAGAACCAGGAACGTCCGGCACTGCCAGATGCCGCCACTCCGCCTGTCCCACTGCTATTTGa                                                                                              >  1:57724/1‑140 (MQ=255)                                          gCTATGCCGGCGTTGTGCAGCGCCTGGCGCATACACTCACACGCCAGTTGCCAGTTTTTGTTGAGATCAAATTCCATAGAACCAGGAACGTCCGGCACTGCCAGATGCCGCCACTCCGCCTGTCCCACTGCTATTTGAtt                                                                                            <  2:57724/140‑1 (MQ=255)                                              tGCCGGCGTTGTGCAGCGCCTGGCGCATACACTCACACGCCAGTTGCCAGTTTTTGTTGAGATCAAATTCCATAGAACCAGGAACGTCCGGCACTGCCAGATGCCGCCACTCCGCCTGTCCCACTGCTATTTGATTGCCt                                                                                        >  2:188393/1‑140 (MQ=255)                                              tGCCGGCGTTGTGCAGCGCCTGGCGCATACACTCACACGCCAGTTGCCAGTTTTTGTTGAGATCAAATTCCATAGAACCAGGAACGTCCGGCACTGCCAGATGCCACCACTCCGCCTGTCCCACTGCTATTTGATTGCCt                                                                                        >  2:641028/1‑140 (MQ=255)                                                               gcCTGGCGCATACACTCACACGCCAGTTGCCAGTTTTTGTTGAGATCAAATTCCATAGAACCAGGAACGTCCGGCACTGCCAGATGCCGCCACTCCGCCTGTCCCACTGCTATTTGATTGCCTTCCAGGTCGAATATcac                                                                       >  1:930125/1‑140 (MQ=255)                                                               gcCTGGCGCATACACTCACACGCCAGTTGCCAGTTTTTGTTGAGATCAAATTCCATAGAACCAGGAACGTCCGGCACTGCCAGATGCCGCCACTCCGCCTGTCCCACTGCTATTTGATTGCCTTCCAGGTCGAATATcac                                                                       <  2:933774/140‑1 (MQ=255)                                                                   ggCGCATACACTCACACGCCAGTTGCCAGTTTTTGTTGAGATCAAATTCCATAGAACCAGGAACGTCCGGCACTGCCAGATGCCGCCACTCCGCCTGTCCCACTGCTATTTGATTGCCTTCCAgg                                                                                  >  1:568627/1‑125 (MQ=255)                                                                   ggCGCATACACTCACACGCCAGTTGCCAGTTTTTGTTGAGATCAAATTCCATAGAACCAGGAACGTCCGGCACTGCCAGATGCCGCCACTCCGCCTGTCCCACTGCTATTTGATTGCCTTCCAgg                                                                                  <  2:568627/125‑1 (MQ=255)                                                                   ggCGCATACACTCACACGCCAGTTGCCAGTTTTTGTTGAGATCAAATTCCATAGAACCAGGAACGTCCGGCACTGCCAGATGCCGCCACTCCGCCTGTCCCACTGCTATTTGATTGCCTTCCAGGTCGAATATCACAGcc                                                                   <  2:930125/140‑1 (MQ=255)                                                                   ggCGCATACACTCACACGCCAGTTGCCAGTTTTTGTTGAGATCAAATTCCATAGAACCAGGAACGTCCGGCACTGCCAGATGCCGCCACTCCGCCTGTCCCACTGCTATTTGATTGCCTTCCAGGTCGAATATCACAGcc                                                                   >  2:740113/1‑140 (MQ=255)                                                                         tACACTCACACGCCAGTTGCCAGTTTTTGTTGAGATCAAATTCCATAGAACCAGGAACGTCCGGCACTGCCAGATGCCGCCACTCCGCCTGTCCCACTGCTATTTGATTGCCTTCCAGGTCGAATATCACAGCCCGAATa                                                             <  1:740113/140‑1 (MQ=255)                                                                               cacaCGCCAGTTGCCAGTTTTTGTTGAGATCAAATTCCATAGAACCAGGAACGTCCGGCACTGCCAGATGCCGCCACTCCGCCTGTCCCACTGCTATTTGATTGCCTTCCAGGTCGAATATCACAGCCCGAATACTTCCg                                                       <  2:387516/140‑1 (MQ=255)                                                                                acacGCCAGTTGCCAGTTTTTGTTGAGATCAAATTCCATAGAACCAGGAACGTCCGGCACTGCCAGATGCCGCCACTCCGCCTGTCCCACTGCTATTTGATTGCCTTCCAGGTCGAATATCACAGCCCGAATACTTCCgg                                                      <  2:278244/140‑1 (MQ=255)                                                                                   cGCCAGTTGCCAGTTTTTGTTGAGATCAAATTCCATAGAACCAGGAACGTCCGGCACTGCCAGATGCCGCCACTCCGCCTGTCCCACTGCTATTTGATTGCCTTCCAGGTCGAATATCACAGCCCGAATACTTCCGGTGc                                                   <  2:6091/140‑1 (MQ=255)                                                                                     ccagttgccagttTTTGTTGAGATCAAATTCCATAGAACCAGGAACGTCCGGCACTGCCAGATGCCGCCACTCCGCCTGTCCCACTGCTATTTGATTGCCTTCCAGGTCGAATATCACAGCCCGAATACTTCCGGTGCCt                                                 >  1:665441/1‑140 (MQ=255)                                                                                     ccagttgccagttTTTGTTGAGATCAAATTCCATAGAACCAGGAACGTCCGGCACTGCCAGATGCCACCACTCCGCCTGTCCCACTGCTATTTGATTGCCTTCCAGGTCGAATATCACAGCCCGAATACTTCCGGTGCCt                                                 <  1:641028/140‑1 (MQ=255)                                                                                        gttgccagttTTTGTTGAGATCAAATTCCATAGAACCAGGAACGTCCGGCACTGCCAGATGCCGCCACTCCGCCTGTCCCACTGCTATTTGATTGCCTTCCAGGTCGAATATCACAGCCCGAATACTTCCGGTGCCTGCa                                              >  2:805065/1‑140 (MQ=255)                                                                                        gttgccagttTTTGTTGAGATCAAATTCCATAGAACCAGGAACGTCCGGCACTGCCAGATGCCGCCACTCCGCCTGTCCCACTGCTATTTGATTGCCTTCCAGGTCGAATATCACAGCCCGAATACTTCCGGTGCCTGCa                                              >  1:313424/1‑140 (MQ=255)                                                                                             cagttTTTGTTGAGATCAAATTCCATAGAACCAGGAACGTCCGGCACTGCCAGATGCCGCCACTCCGCCTGTCCCACTGCTATTTGATTGCCTTCCAGGTCGAATATCACAGCCCGAATACTTCCGGTGCCTGCATCCAg                                         <  2:164107/140‑1 (MQ=255)                                                                                             cagttTTTGTTGAGATCAAATTCCATAGAACCAGGAACGTCCGGCACTGCCAGATGCCGCCACTCCGCCTGTCCCACTGCTATTTGATTGCCTTCCAGGTCGAATATCACAGCCCGAATACTTCCGGTGCCTGCATCCAg                                         <  2:618829/140‑1 (MQ=255)                                                                                                  tttGTTGAGATCAAATTCCATAGAACCAGGAACGTCCGGCACTGCCAGATGCCGCCACTCCGCCTGTCCCACTGCTATTTGATTGCCTTCCAGGTCGAATATCACAGCCCGAATACTTCCGGTGCCTGCATCCAGCGCCa                                    <  2:738783/140‑1 (MQ=255)                                                                                                       tgAGATCAAATTCCATAGAACCAGGAACGTCCGGCACTGCCAGATGCCGCCACTCCGCCTGTCCCACTGCTATTTGATTGCCTTCCAGGTCGAATATCACAGCCCGAATACTTCCGGTGCCTGCATCCAGCGCCATCAgg                               <  2:454500/140‑1 (MQ=255)                                                                                                        gagaTCAAATTCCATAGAACCAGGAACGTCCGGCACTGCCAGATGCCGCCACTCCGCCTGTCCCACTGCTATTTGATTGCCTTCCAGGTCGAATATCACAGCCCGAATACTTCCGGTGCCTGCATCCAGCGCCATCAGgt                              <  1:805065/140‑1 (MQ=255)                                                                                                           aTCAAATTCCATAGAACCAGGAACGTCCGGCACTGCCAGATGCCGCCACTCCGCCTGTCCCACTGCTATTTGATTGCCTTCCAGGTCGAATATCACAGCCCGAATACTTCCGGTGCCTGCATCCAGCGCCATCAGgtagt                           >  1:165940/1‑140 (MQ=255)                                                                                                           aTCAAATTCCATAGAACCAGGAACGTCCGGCACTGCCAGATGCCGCCACTCCGCCTGTCCCACTGCTATTTGATTGCCTTCCAGGTCGAATATCACAGCCCGAATACTTCCGGTGCCTGCATCCAGCGCCATCAGgtagt                           >  1:912374/1‑140 (MQ=255)                                                                                                           aTCAAATTCCATAGAACCAGGAACGTCCGGCACTGCCAGATGCCGCCACTCCGCCTGTCCCACTGCTATTTGATTGCCTTCCAGGTCGAATATCACAGCCCGAATACTTCCGGTGCCTGCATCCAGCGCCATCAGgtagt                           >  1:415788/1‑140 (MQ=255)                                                                                                                         aaCCAGGAACGTCCGGCACTGCCAGATGCCGCCACTCCGCCTGTCCCACTGCTATTTGATTGCCTTCCAGGTCGAATATCACAGCCCGAATACTTCCGGTGCCTGCATCCAGCGCCATCAGGTAGTACTTTGATTCTGaa             >  1:629469/1‑140 (MQ=255)                                                                                                                              ggAACGTCCGGCACTGCCAGATGCCGCCACTCCGCCTGTCCCACTGCTATTTGATTGCCTTCCAGGTCGAATATCACAGCCCGAATACTTCCGGTGCCTGCATCCAGCGCCATCAGGTAGTACTTTGATTCTGaaa            >  2:601134/1‑136 (MQ=255)                                                                                                                              ggAACGTCCGGCACTGCCAGATGCCGCCACTCCGCCTGTCCCACTGCTATTTGATTGCCTTCCAGGTCGAATATCACAGCCCGAATACTTCCGGTGCCTGCATCCAGCGCCATCAGGTAGTACTTTGATTCTGaaa            <  1:601134/136‑1 (MQ=255)                                                                                                                                    tCCGGCACTGCCAGATGCCGCCACTCCGCCTGTCCCACTGCTATTTGATTGCCTTCCAGGTCGAATATCACAGCCCGAATACTTCCGGTGCCTGCATCCAGCGCCATCAGGTAGTACTTTGATTCTGAAAGGGTAAagag  >  2:942302/1‑140 (MQ=255)                                                                                                                                         |                                                                                                                                       CACGCATCGAACATGCCGAAACGGCAGCGATATACTCCGGGGCTATGCCGGCGTTGTGCAGCGCCTGGCGCATACACTCACACGCCAGTTGCCAGTTTTTGTTGAGATCAAATTCCATAGAACCAGGAACGTCCGGTACTGCCAGATGCCGCCACTCCGCCTGTCCCACTGCTATTTGATTGCCTTCCAGGTCGAATATCACAGCCCGAATACTTCCGGTGCCTGCATCCAGCGCCATCAGGTAGTACTTTGATTCTGAAAGGGTAAAGAG  >  NC_000913/1599930‑1600200

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑