Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,605,121	T→C	V24A (GTG→GCG)	lsrB →	Autoinducer‑2 ABC transporter periplasmic binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,605,121	0	T	C	100.0%	181.1 / NA	51	V24A (GTG→GCG)	lsrB	Autoinducer‑2 ABC transporter periplasmic binding protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (27/24);  total (27/24)

TCGCCAGCAAATTAAAGAGTGGCTGGCGCGTCGGGCCAATAACCCATTGCCATAAAGGATATCTTCATGACACTTCATCGCTTTAAGAAAATCGCCTTACTTAGCGCTCTTGGCATTGCCGCAATCTCTATGAATGTGCAGGCCGCAGAGCGTATTGCATTTATTCCCAAACTGGTTGGCGTGGGATTTTTTACCAGCGGTGGCAACGGCGCACAACAAGCGGGTAAAGAGCTGGGCGTTGATGTGACCTACGACGGGCCGACAGAACCCAGT  >  NC_000913/1604985‑1605257                                                                                                                                         |                                                                                                                                         tCGCCAGCAAATTAAAGAGTGGCTGGCGCGTCGGGCCAATAACCCATTGCCATAAAGGATATCTTCATGACACTTCATCGCTTTAAGAAAATCGCCTTACTTAGCGCTCTTGGCATTGCCGCAATCTCTATGAATGCGCa                                                                                                                                       <  1:487200/140‑1 (MQ=255)    ccAGCAAATTAAAGAGTGGCTGGCGCGTCGGGCCAATAACCCATTGCCATAAAGGATATCTTCATGACACTTCATCGCTTTAAGAAAATCGCCTTACTTAGCGCTCTTGGCATTGCCGCAATCTCTATGAATGCGCAGGc                                                                                                                                    >  1:588433/1‑140 (MQ=255)    ccAGCAAATTAAAGAGTGGCTGGCGCGTCGGGCCAATAACCCATTGCCATAAAGGATATCTTCATGACACTTCATCGCTTTAAGAAAATCGCCTTACTTAGCGCTCTTGGCATTGCCGCAATCTCTATGAATGCGCAGGc                                                                                                                                    >  1:307743/1‑140 (MQ=255)       gCAAATTAAAGAGTGGCTGGCGCGTCGGGCCAATAACCCATTGCCATAAAGGATATCTTCATGACACTTCATCGCTTTAAGAAAATCGCCTTACTTAGCGCTCTTGGCATTGCCGCAATCTCTATGAATGCGCAGGCCGc                                                                                                                                 >  1:228807/1‑140 (MQ=255)        cAAATTAAAGAGTGGCTGGCGCGTCGGGCCAATAACCCATTGCCATAAAGGATATCTTCATGACACTTCATCGCTTTAAGAAAATCGCCTTACTTAGCGCTCTTGGCATTGCCGCAATCTCTATGAATGCGCAGGCCGCa                                                                                                                                >  2:912483/1‑140 (MQ=255)                      gctggcGCGTCGGGCCAATAACCCATTGCCATAAAGGATATCTTCATGACACTTCATCGCTTTAAGAAAATCGCCTTACTTAGCGCTCTTGGCATTGCCGCAATCTCTATGAATGCGCAGGCCGCa                                                                                                                                >  2:305704/1‑126 (MQ=255)                      gctggcGCGTCGGGCCAATAACCCATTGCCATAAAGGATATCTTCATGACACTTCATCGCTTTAAGAAAATCGCCTTACTTAGCGCTCTTGGCATTGCCGCAATCTCTATGAATGCGCAGGCCGCa                                                                                                                                <  1:305704/126‑1 (MQ=255)                      gctggcGCGTCGGGCCAATAACCCATTGCCATAAAGGATATCTTCATGACACTTCATCGCTTTAAGAAAATCGCCTTACTTAGCGCTCTTGGCATTGCCGCAATCTCTATGAATGCGCAGGCCGCAGAGCGTATTGCAtt                                                                                                                  >  1:893069/1‑140 (MQ=255)                           cgcgTCGGGCCAATAACCCATTGCCATAAAGGATATCTTCATGACACTTCATCGCTTTAAGAAAATCGCCTTACTTAGCGCTCTTGGCATTGCCGCAATCTCTATGAATGCGCAGGCCGCAGAGCGTATTACATTTATTc                                                                                                             <  1:253104/140‑1 (MQ=255)                             cgTCGGGCCAATAACCCATTGCCATAAAGGATATCTTCATGACACTTCATCGCTTTAAGAAAATCGCCTTACTTAGCGCTCTTGGCATTGCCGCAATCTCTATGAATGCGCAGGCCGCAGAGCGTATTGCATTTATTccc                                                                                                           <  1:190595/140‑1 (MQ=255)                                     cAATAACCCATTGCCATAAAGGATATCTTCATGACACTTCATCGCTTTAAGAAAATCGCCTTACTTAGCGCTCTTGGCATTGCCGCAATCTCTATGAATGCGCAGGCCGCAGAGCGTATTGCATTTATTCCCAAACTGGt                                                                                                   <  1:912483/140‑1 (MQ=255)                                             cATTGCCATAAAGGATATCTTCATGACACTTCATCGCTTTAAGAAAATCGCCTTACTTAGCGCTCTTGGCATTGCCGCAATCTCTATGAATGCGCAGGCCGCAGAGCGTATTGCATTTATTCCCAAACTGGTTGGCGTgg                                                                                           <  2:307743/140‑1 (MQ=255)                                             cATTGCCATAAAGGATATCTTCATGACACTTCATCGCTTTAAGAAAATCGCCTTACTTAGCGCTCTTGGCATTGCCGCAATCTCTATGAATGCGCAGGCCGCAGAGCGTATTGCATTTATTCCCAAACTGGTTGGCGTgg                                                                                           >  2:250603/1‑140 (MQ=255)                                             cATTGCCATAAAGGATATCTTCATGACACTTCATCGCTTTAAGAAAATCGCCTTACTTAGCGCTCTTGGCATTGCCGCAATCTCTATGAATGCGCAGGCCGCAGAGCGTATTGCATTTATTCCCAAACTGGTTGGCGTgg                                                                                           >  2:133932/1‑140 (MQ=255)                                                 gCCATAAAGGATATCTTCATGACACTTCATCGCTTTAAGAAAATCGCCTTACTTAGCGCTCTTGGCATTGCCGCAATCTCTATGAATGCGCAGGCCGCAGAGCGTATTGCATTTATTCCCAAACTGGTTGGCGTGGGAtt                                                                                       >  2:288086/1‑140 (MQ=255)                                                     tAAAGGATATCTTCATGACACTTCATCGCTTTAAGAAAATCGCCTTACTTAGCGCTCTTGGCATTGCCGCAATCTCTATGAATGCGCAGGCCGCAGAGCGTATTGCATTTATTCCCAAACTGGTTGGCGTGGGAtttttt                                                                                   >  2:600708/1‑140 (MQ=255)                                                     tAAAGGATATCTTCATGACACTTCATCGCTTTAAGAAAATCGCCTTACTTAGCGCTCTTGGCATTGCCGCAATCTCTATGAATGCGCAGGCCGCAGAGCGTATTGCATTTATTCCCAAACTGGTTGGCGTGGGAtttttt                                                                                   >  2:824370/1‑140 (MQ=255)                                                            tatCTTCATGACACTTCATCGCTTTAAGAAAATCGCCTTACTTAGCGCTCTTGGCATTGCCGCAATCTCTATGAATGCGCAGGCCGCAGAGCGTATTGCATTTACTCCCAAACTGGTTGGCGTGGGATTTTTTACCAGCg                                                                            >  2:569423/1‑140 (MQ=255)                                                                   aTGACACTTCATCGCTTTAAGAAAATCGCCTTACTTAGCGCTCTTGGCATTGCCGCAATCTCTATGAATGCGCAGGCCGCAGAGCGTATTGCATTTATTCCCAAACTGGTTGGCGTGGGATTTTTTACCAGCGGTGGCaa                                                                     <  2:228807/140‑1 (MQ=255)                                                                   aTGACACTTCATCGCTTTAAGAAAATCGCCTTACTTAGCGCTCTTGGCATTGCCGCAATCTCTATGAATGCGCAGGCCGCAGAGCGTATTGCATTTATTCCCAAACTGGTTGGCGTGGGATTTTTTACCAGCGGTGGCaa                                                                     <  2:214495/140‑1 (MQ=255)                                                                    tGACACTTCATCGCTTTAAGAAAATCGCCTTACTTAGCGCTCTTGGCATTGCCGCAATCTCTATGAATGCGCAGGCCGCAGAGCGTATTGCATTTATTCCCAAACTGGTTGGCGTGGGATTTTTTACCAGCGGTGGCaa                                                                     >  2:467614/1‑139 (MQ=255)                                                                    tGACACTTCATCGCTTTAAGAAAATCGCCTTACTTAGCGCTCTTGGCATTGCCGCAATCTCTATGAATGCGCAGGCCGCAGAGCGTATTGCATTTATTCCCAAACTGGTTGGCGTGGGATTTTTTACCAGCGGTGGCaa                                                                     <  1:467614/139‑1 (MQ=255)                                                                    tGACACTTCATCGCTTTAAGAAAATCGCCTTACTTAGCGCTCTTGGCATTGCCGCAATCTCTATGAATGCGCAGGCCGCAGAGCGTATTGCATTTATTCCCAAACTGGTTGGCGTGGGATTTTTTACCAGCGGTGGCAAc                                                                    >  1:545883/1‑140 (MQ=255)                                                                        acTTCATCGCTTTAAGAAAATCGCCTTACTTAGCGCTCTTGGCATTGCCGCAATCTCTATGAATGCGCAGGCCGCAGAGCGTATTGCATTTATTCCCAAACTGGTTGGCGTGGGATTTTTTACCAGCGGTGGCAACGgcg                                                                <  1:611795/140‑1 (MQ=255)                                                                        acTTCATCGCTTTAAGAAAATCGCCTTACTTAGCGCTCTTGGCATTGCCGCAATCTCTATGAATGCGCAGGCCGCAGAGCGTATTGCATTTATTCCCAAACTGGTTGGCGTGGGATTTTTTACCAGCGGTGGCAACGgcg                                                                <  2:228624/140‑1 (MQ=255)                                                                        acTTCATCGCTTTAAGAAAATCGCCTTACTTAGCGCTCTTGGCATTGCCGCAATCTCTATGAATGCGCAGGCCGCAGAGCGTATTGCATTTATTCCCAAACTGGTTGGCGTGGGATTTTTTACCAGCGGTGGCAACGgcg                                                                <  2:228623/140‑1 (MQ=255)                                                                         cTTCATCGCTTTAAGAAAATCGCCTTACTTAGCGCTCTTGGCATTGCCGCAATCTCTATGAATGCGCAGGCCGCAGAGCGTATTGCATTTATTCCCAAACTGGTTGGCGTGGGATTTTTTACCAGCGGTGGCAACGgcgc                                                               >  1:563718/1‑140 (MQ=255)                                                                          ttCATCGCTTTAAGAAAATCGCCTTACTTAGCGCTCTTGGCATTGCCGCAATCTCTATGAATGCGCAGGCCGCAGAGCGTATTGCATTTATTCCCAAACTGGTTGGCGTGGGATTTTTTACCAGCGGTGGCAACGGCGca                                                              >  2:952484/1‑140 (MQ=255)                                                                          ttCATCGCTTTAAGAAAATCGCCTTACTTAGCGCTCTTGGCATTGCCGCAATCTCTATGAATGCGCAGGCCGCAGAGCGTATTGCATTTATTCCCAAACTGGTTGGCGTGGGATTTTTTACCAGCGGTGGCAACGGCGca                                                              >  2:707129/1‑140 (MQ=255)                                                                          ttCATCGCTTTAAGAAAATCGCCTTACTTAGCGCTCTTGGCATTGCCGCAATCTCTATGAATGCGCAGGCCGCAGAGCGTATTGCATTTATTCCCAAACTGGTTGGCGTGGGATTTTTTACCAGCGGTGGCAACGGCGca                                                              >  1:267907/1‑140 (MQ=255)                                                                          ttCATCGCTTTAAGAAAATCGCCTTACTTAGCGCTCTTGGCATTGCCGCAATCTCTATGAATGCGCAGGCCGCAGAGCGTATTGCATTTATTCCCAAACTGGTTGGCGTGGGATTTTTTACCAGCGGTGGCAACGGCGca                                                              >  2:11911/1‑140 (MQ=255)                                                                            cATCGCTTTAAGAAAATCGCCTTACTTAGCGCTCTTGGCATTGCCGCAATCTCTATGAATGCGCAGGCCGCAGAGCGTATTGCATTTATTCCCAAACTGGTTGGCGTGGGATTTTTTACCAGCGGTGGCAACGGCGcaca                                                            >  1:568546/1‑140 (MQ=255)                                                                               cGCTTTAAGAAAATCGCCTTACTTAGCGCTCTTGGCATTGCCGCAATCTCTATGAATGCGCAGGCCGCAGAGCGTATTGCATTTATTCCCAAACTGGTTGGCGTGGGATTTTTTACCAGCGGTGGCAACGGCGCacaaca                                                         <  1:133932/140‑1 (MQ=255)                                                                               cGCTTTAAGAAAATCGCCTTACTTAGCGCTCTTGGCATTGCCGCAATCTCTATGAATGCGCAGGCCGCAGAGCGTATTGCATTTATTCCCAAACTGGTTGGCGTGGGATTTTTTACCAGCGGTGGCAACGGCGCacaaca                                                         <  2:588433/140‑1 (MQ=255)                                                                                 cTTTAAGAAAATCGCCTTACTTAGCGCTCTTGGCATTGCCGCAATCTCTATGAATGCGCAGGCCGCAGAGCGTATTGCATTTATTCCCAAACTGGTTGGCGTGGGATTTTTTACCAGCGGTGGCAACGGCGCACAACAAg                                                       <  1:707129/140‑1 (MQ=255)                                                                                 cTTTAAGAAAATCGCCTTACTTAGCGCTCTTGGCATTGCCGCAATCTCTATGAATGCGCAGGCCGCAGAGCGTATTGCATTTATTCCCAAACTGGTTGGCGTGGGATTTTTTACCAGCGGTGGCAACGGCGCACAACAAg                                                       >  1:799094/1‑140 (MQ=255)                                                                                  tttAAGAAAATCGCCTTACTTAGCGCTCTTGGCATTGCCGCAATCTCTATGAATGCGCAGGCCGCAGAGCGTATTGCATTTATTCCCAAACTGGTTGGCGTGGGATTTTTTACCAGCGGTGGCAACGGCGCACAACAAGc                                                      >  1:140894/1‑140 (MQ=255)                                                                                  tttAAGAAAATCGCCTTACTTAGCGCTCTTGGCATTGCCGCAATCTCTATGAATGCGCAGGCCGCAGAGCGTATTGCATTTATTCCCAAACTGGTTGGCGTGGGATTTTTTACCAGCGGTGGCAACGGCGCACAACAAGc                                                      <  1:952484/140‑1 (MQ=255)                                                                                      aGAAAATCGCCTTACTTAGCGCTCTTGGCATTGCCGCAATCTCTATGAATGCGCAGGCCGCAGAGCGTATTGCATTTATTCCCAAACTGGTTGGCGTGGGATTTTTTACCAGCGGTGGCAACGGCGCACAACAAGCGGGt                                                  >  1:324360/1‑140 (MQ=255)                                                                                                   acttcGCGCTCTTGGCATTGCCGCAATCTCTATGAATGCGCAGGCCGCAGAGCGTATTGCATTTATTCCCAAACTGGTTGGCGTGGGATTTTTTACCAGCGGTGGCAACGGCGCACAACAAGCGGGTAAAGAGCTGGGCg                                     <  2:893069/140‑1 (MQ=255)                                                                                                   acttaGCGCTCTTGGCATTGCCGCAATCTCTATGAATGCGCAGGCCGCAGAGCGTATTGCATTTATTCCCAAACTGGTTGGCGTGGGATTTTTTACCAGCGGTGGCAACGGCGCACAACAAGCGGGTAAAGAGCTGGGCg                                     <  2:799094/140‑1 (MQ=255)                                                                                                   acttaGCGCTCTTGGCATTGCCGCAATCTCTATGAATGCGCAGGCCGCAGAGCGTATTGCATTTATTCCCAAACTGGTTGGCGTGGGATTTTTTACCAGCGGTGGCAACGGCGCACAACAAGCGGGTAAAGAGCTGGGCg                                     <  2:202134/140‑1 (MQ=255)                                                                                                     ttaGCGCTCTTGGCATTGCCGCAATCTCTATGAATGCGCAGGCCGCAGAGCGTATTGCATTTATTCCCAAACTGGTTGGCGTGGGATTTTTTACCAGCGGTGGCAACGGCGCACAACAAGCGGGTAAAGAGCTGGGCGtt                                   >  2:319600/1‑140 (MQ=255)                                                                                                         cgcTCTTGGCATTGCCGCAATCTCTATGAATGCGCAGGCCGCAGAGCGTATTGCATTTATTCCCAAACTGGTTGGCGTGGGATTTTTTACCAGCGGTGGCAACGGCGCACAACAAGCGGGTAAAGAGCTGGGCGTTGAtg                               <  1:824370/140‑1 (MQ=255)                                                                                                         cgcTCTTGGCATTGCCGCAATCTCTATGAATGCGCAGGCCGCAGAGCGTATTGCATTTATTCCCAAACTGGTTGGCGTGGGATTTTTTACCAGCGGTGGCAACGGCGCACAACAAGCGGGTAAAGAGCTGGGCGTTGAtg                               <  1:600708/140‑1 (MQ=255)                                                                                                         cgcTCTTGGCATTGCCGCAATCTCTATGAATGCGCAGGCCGCAGAGCGTATTGCATTTATTCCCAAACTGGTTGGCGTGGGATTTTTTACCAGCGGTGGCAACGGCGCACAACAAGCGGGTAAAGAGCTGGGCGTTGAtg                               >  2:61669/1‑140 (MQ=255)                                                                                                         cgcTCTTGGCATTGCCGCAATCTCTATGAATGCGCAGGCCGCAGAGCGTATTGCATTTATTCCCAAACTGGTTGGCGTGGGATTTTTTACCAGCGGTGGCAACGGCGCACAACAAGCGGGTAAAGAGCTGGGCGTTGAtg                               <  2:140894/140‑1 (MQ=255)                                                                                                                   aTTGCCGCAATCTCTATGAATGCGCAGGCCGCAGAGCGTATTGCATTTATTCCCAAACTGGTTGGCGTGGGATTTTTTACCAGCGGTGGCAACGGCGCACAACAAGCGGGTAAAGAGCTGGGCGTTGATGTGACCTacga                     <  1:250603/140‑1 (MQ=255)                                                                                                                     tGCCGCAATCTCTATGAATGCGCAGGCCGCAGAGCGTATTGCATTTATTCCCAAACTGGTTGGCGTGGGATTTTTTACCAGCGGTGGCAACGGCGCACAACAAGCGGGTAAAGAGCTGGGCGTTGATGTGACCTacgacg                   >  2:723698/1‑140 (MQ=255)                                                                                                                      gCCGCAATCTCTATGAATGCGCAGGCCGCAGAGCGTATTGCATTTATTCCCAAACTGGTTGGCGTGGGATTTTTTACCAGCGGTGGCAACGGCGCACAACAAGCGGGTAAAGAGCTGGGCGTTGATGTGACCTACGACgg                  <  2:563718/140‑1 (MQ=255)                                                                                                                                      aTGCGCAGGCCGCAGAGCGTATTGCATTTATTCCCAAACTGGTTGGCGTGGGATTTTTTACCAGCGGTGGCAACGGCGCACAACAAGCGGGTAAAGAGCTGGGCGTTGATGTGACCTACGACGGGCCGACAGAACCCAgt  >  2:506456/1‑140 (MQ=255)                                                                                                                                         |                                                                                                                                         TCGCCAGCAAATTAAAGAGTGGCTGGCGCGTCGGGCCAATAACCCATTGCCATAAAGGATATCTTCATGACACTTCATCGCTTTAAGAAAATCGCCTTACTTAGCGCTCTTGGCATTGCCGCAATCTCTATGAATGTGCAGGCCGCAGAGCGTATTGCATTTATTCCCAAACTGGTTGGCGTGGGATTTTTTACCAGCGGTGGCAACGGCGCACAACAAGCGGGTAAAGAGCTGGGCGTTGATGTGACCTACGACGGGCCGACAGAACCCAGT  >  NC_000913/1604985‑1605257

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑