Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,668,519 T→C T16A (ACC→GCC)  mlc ← DNA‑binding transcriptional repressor Mlc

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,668,5190TC97.2% 120.9 / ‑4.9 36T16A (ACC→GCC) mlcDNA‑binding transcriptional repressor Mlc
Reads supporting (aligned to +/- strand):  ref base T (1/0);  new base C (17/18);  total (18/18)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01

CAGGTGTGCTTCGAGCATCTCACGGACAATTTTAGTGATACTGGCAGGAGCCAGTTGCGCCAGACGGGAAAGATCGATACGCGAGACTGGACCAAGCTGATCAATCAGGCGATAAACCGCGCCCGCGTTGGTCTGCTTTATTTGATCAATGTGCCCAGGCTGGTTTTCAGCAACCACCGCATACTCCCTATATTTTCGCGCTCCGAAATAATCTGTAGGCTATGGTGAAGCACTTCAATACGTGTCGTCAAATTTTTACTTAGGC  >  NC_000913/1668388‑1668652
                                                                                                                                   |                                                                                                                                     
cAGGTGTGCTTCGAGCATCTCACGGACAATTTTAGTGATACTGGCAGGAGCCAGTTGCGCCAGACGGGAAAGATCGATACGCGAGACTGGACCAAGCTGATCAATCAGGCGATAAACCGCGCCCGCGTTGGCCTGCTTTa                                                                                                                               <  1:569608/140‑1 (MQ=255)
            gAGCATCTCACGGACAATTTTAGTGATACTGGCAGGAGCCAGTTGCGCCAGACGGGAAAGATCGATACGCGAGACTGGACCAAGCTGATCAATCAGGCGATAAACCGCGCCCGCGTTGGCCTGCTTTATTTGATCAAtgg                                                                                                                   >  2:770946/1‑139 (MQ=255)
             aGCATCTCACGGACAATTTTAGTGATACTGGCAGGAGCCAGTTGCGCCAGACGGGAAAGATCGATACGCGAGACTGGACCAAGCTGATCAATCAGGCGATAAACCGCGCCCGCGTTGGCCTGCTTTATTTGATCAAtgtg                                                                                                                  >  2:684028/1‑140 (MQ=255)
             aGCATCTCACGGACAATTTTAGTGATACTGGCAGGAGCCAGTTGCGCCAGACGGGAAAGATCGATACGCGAGACTGGACCAAGCTGATCAATCAGGCGATAAACCGCGCCCGCGTTGGCCTGCTTTATTTGATCAAtgtg                                                                                                                  >  1:904926/1‑140 (MQ=255)
             aGCATCTCACGGACAATTTTAGTGATACTGGCAGGAGCCAGTTGCGCCAGACGGGAAAGATCGATACGCGAGACTGGACCAAGCTGATCAATCAGGCGATAAACCGCGCCCGCGTTGGCCTGCTTTATTTGATCAAtgtg                                                                                                                  >  2:848640/1‑140 (MQ=255)
             aGCATCTCACGGACAATTTTAGTGATACTGGCAGGAGCCAGTTGCGCCAGACGGGAAAGATCGATACGCGAGACTGGACCAAGCTGATCAATCAGGCGATAAACCGCGCCCGCGTTGGCCTGCTTTATTTGATCAAt                                                                                                                     <  2:525759/137‑1 (MQ=255)
             aGCATCTCACGGACAATTTTAGTGATACTGGCAGGAGCCAGTTGCGCCAGACGGGAAAGATCGATACGCGAGACTGGACCAAGCTGATCAATCAGGCGATAAACCGCGCCCGCGTTGGCCTGCTTTATTTGATCAAt                                                                                                                     >  1:525759/1‑137 (MQ=255)
                          cAATTTTAGTGATACTGGCAGGAGCCAGTTGCGCCAGACGGGAAAGATCGATACGCGAGACTGGACCAAGCTGAACAATCAGGCGATAAACCGCGCCCGCGTTGGCCTGCTTTATTTGATCAATGTGCCCAGGCTGGttt                                                                                                     <  1:848640/140‑1 (MQ=255)
                             ttttAGTGATACTGGCAGGAGCCAGTTGCGCCAGACGGGAAAGATCGATACGCGAGACTGGACCAAGCTGATCAATCAGGCGATAAACCGCGCCCGCGTTGGCCTGCTTTATTTGATCAATGTGCCCAGGCTGGTTTTCa                                                                                                  >  1:835911/1‑140 (MQ=255)
                               ttAGTGATACTGGCAGGAGCCAGTTGCGCCAGACGGGAAAGATCGATACGCGAGACTGGACCAAGCTGATCAATCAGGCGATAAACCGCGCCCGCGTTGGCCTGCTTTATTTGATCAATGTGCCCAGGCTGGTTTTCAGc                                                                                                <  2:702039/140‑1 (MQ=255)
                                 aGTGATACTGGCAGGAGCCAGTTGCGCCAGACGGGAAAGATCGATACGCGAGACTGGACCAAGCTGATCAATCAGGCGATAAACCGCGCCCGCGTTGGCCTGCTTTATTTGATCAATGTGCCCAGGCTGGTTTTCAGCaa                                                                                              <  2:557451/140‑1 (MQ=255)
                                       aCTGGCAGGAGCCAGTTGCGCCAGACGGGAAAGATCGATACGCGAGACTGGACCAAGCTGATCAATCAGGCGATAAACCGCGCCCGCGTTGGCCTGCTTTATTTGATCAATGTGCCCAGGCTGGtttt                                                                                                    >  2:91413/1‑128 (MQ=255)
                                       aCTGGCAGGAGCCAGTTGCGCCAGACGGGAAAGATCGATACGCGAGACTGGACCAAGCTGATCAATCAGGCGATAAACCGCGCCCGCGTTGGCCTGCTTTATTTGATCAATGTGCCCAGGCTGGtttt                                                                                                    <  1:91413/128‑1 (MQ=255)
                                       aCTGGCAGGAGCCAGTTGCGCCAGACGGGAAAGATCGATACGCGAGACTGGACCAAGCTGATCAATCAGGCGATAAACCGCGCCCGCGTTGGCCTGCTTTATTTGATCAATGTGCCCAGGCTGGTTTTCAGCAACCACCg                                                                                        >  2:166187/1‑140 (MQ=255)
                                       aCTGGCAGGAGCCAGTTGCGCCAGACGGGAAAGATCGATACGCGAGACTGGACCAAGCTGATCAATCAGGCGATAAACCGCGCCCGCGTTGGCCTGCTTTATTTGATCAATGTGCCCAGGCTGGTTTTCAGCAACCACCg                                                                                        >  2:257631/1‑140 (MQ=255)
                                       aCTGGCAGGAGCCAGTTGCGCCAGACGGGAAAGATCGATACGCGAGACTGGACCAAGCTGATCAATCAGGCGATAAACCACGCCCGCGTTGGCCTGCTTTATTTGATCAATGTGCCCAGGCTGGTTTTCAGCAACCACCg                                                                                        >  2:600592/1‑140 (MQ=255)
                                        cTGGCAGGAGCCAGTTGCGCCAGACGGGAAAGATCGATACGCGAGACTGGACCCAGCTGATCAATCAGGCGATAAACCGCGCCCGCGTTGGTCTGCTTTATTTGATCAATGTGCCCAGGCTGGGTTTCAGCAACCACCGc                                                                                       >  2:530412/1‑140 (MQ=255)
                                          ggCAGGAGCCAGTTGCGCCAGACGGGAAAGATCGATACGCGAGACTGGACCAAGCTGATCAATCAGGCGATAAACCGCGCCCGCGTTGGCCTGCTTTATTTGATCAATGTGCCCAGGCTGGTTTTCAGCAACCACCGCAt                                                                                     <  1:166187/140‑1 (MQ=255)
                                           gCAGGAGCCAGTTGCGCCAGACGGGAAAGATCGATACGCGAGACTGGACCAAGCTGATCAATCAGGCGATAAACCGCGCCCGCGTTGGCCTGCTTTATTTGATCAATGTGCCCAGGCTGGTTTTCAGCAACCACCGCATa                                                                                    <  1:521980/140‑1 (MQ=255)
                                                aGCCAGTTGCGCCAGACGGGAAAGATCGATACGCGAGACTGGACCAAGCTGATCAATCAGGCGATAAACCGCGCCCGCGTTGGCCTGCTTTATTTGATCAATGTGCCCAGGCTGGTTTTCAGCAACCACCGCATACTccc                                                                               >  2:492647/1‑140 (MQ=255)
                                                  ccAGTTGCGCCAGACGGGAAAGATCGATACGCGAGACTGGACCAAGCTGATCAATCAGGCGATAAACCGCGCCCGCGTTGGCCTGCTTTATTTGATCAATGTGCCCAGGCTGGTTTTCAGCAACCACCGCAt                                                                                     >  1:829015/1‑132 (MQ=255)
                                                  ccAGTTGCGCCAGACGGGAAAGATCGATACGCGAGACTGGACCAAGCTGATCAATCAGGCGATAAACCGCGCCCGCGTTGGCCTGCTTTATTTGATCAATGTGCCCAGGCTGGTTTTCAGCAACCACCGCAt                                                                                     <  2:829015/132‑1 (MQ=255)
                                                          gcCAGACGGGAAAGATCGATACGCGAGACTGGACCAAGCTGATCAATCAGGCGATAAACCGCGCCCGCGTTGGCCTGCTTTATTTGATCAATGTGCCCAGGCTGGTTTTCAGCAACCACCGCATACTCCCTATATTTTcg                                                                     <  1:492647/140‑1 (MQ=255)
                                                          gcCAGACGGGAAAGATCGATACGCGAGACTGGACCAAGCTGATCAATCAGGCGATAAACCGCGCCCGCGTTGGCCTGCTTTATTTGATCAATGTGCCCAGGCTGGTTTTCAGCAACCACCGCATACTCCCTATATTTTcg                                                                     >  2:588437/1‑140 (MQ=255)
                                                              gACGGGAAAGATCGATACGCGAGACTGGACCAAGCTGATCAATCAGGCGATAAACCGCGCCCGCGTTGGCCTGCTTTATTTGATCAATGTGCCCAGGCTGGTTTTCAGCAACCACCGCATACTCCCTATATTTTCGCGCt                                                                 <  2:835911/140‑1 (MQ=255)
                                                                           gctACGCGAGACTGGACCAAGCTGGTCAAGCAAGCGATAAACCGCGCGCGCGGTGGCCGGCGTGCTTTGTTGAAAGAGCCCCGGCTAGCTTTCAGCAACACGCGCATATTCTCTATATTTTCGCGCGCCGAAATAAtgtg                                                    >  2:116132/3‑140 (MQ=255)
                                                                            aTACGCGAGACTGGACCAAGCTGATCAATCAGGCGATAAACCGCGCCCGCGTTGGCCTGCTTTATTTGATCAATGTGCCCAGGCTGGTTTTCAGCAACCACCGCATACTCCCTATATTTTCGCGCTCCGAaata                                                         >  2:435846/1‑134 (MQ=255)
                                                                            aTACGCGAGACTGGACCAAGCTGATCAATCAGGCGATAAACCGCGCCCGCGTTGGCCTGCTTTATTTGATCAATGTGCCCAGGCTGGTTTTCAGCAACCACCGCATACTCCCTATATTTTCGCGCTCCGAaata                                                         <  1:435846/134‑1 (MQ=255)
                                                                               cgcgAGACTGGACCAAGCTGATCAATCAGGCGATAAACCGCGCCCGCGTTGGCCTGCTTTATTTGATCAATGTGCCCAGGCTGGTTTTCAGCAACCACCGCATACTCCCTATATTTTCGCGCTCCGAAATAATCTGTAgg                                                <  1:257631/140‑1 (MQ=255)
                                                                                  gagaCTGGACCAAGCTGATCAATCAGGCGATAAACCGCGCCCGCGTTGGCCTGCTTTATTTGATCAATGTGCCCAGGCTGGTTTTCAGCAACCACCGCATACTCCCTATATTTTCGCGCTCCGAAATAATCTGTAGGCTa                                             <  1:686324/140‑1 (MQ=255)
                                                                                       tGGACCAAGCTGATCAATCAGGCGATAAACCGCGCCCGCGTTGGCCTGCTTTATTTGATCAATGTGCCCAGGCTGGTTTTCAGCAACCACCGCATACTCCCTATATTTTCGCGCTCCGAAATAATCTGTAGGCTATGGTg                                        >  2:813713/1‑140 (MQ=255)
                                                                                             aaGCTGATCAATCAGGCGATAAACCGCGCCCGCGTTGGCCTGCTTTATTTGATCAATGTGCCCAGGCTGGTTTTCAGCAACCACCGCATACTCCCTATATTTTCGCGCTCCGAAATAATCTGTAGGCTATGGTGAAGCAc                                  <  1:684028/140‑1 (MQ=255)
                                                                                                    tcaatcaGGCGATAAACCGCGCCCGCGTTGGCCTGCTTTATTTGATCAATGTGCCCAGGCTGGTTTTCAGCAACCACCGCATACTCCCTATATTTTCGCGCTCCGAAATAATCTGTAGGCTATGGTGAAGCACTTCAATa                           >  1:9749/1‑140 (MQ=255)
                                                                                                           ggCGATAAACCGCGCCCGCGTTGGCCTGCTTTATTTGATCAATGTGCCCAGGCTGGTTTTCAGCAACCACCGCATACTCCCTATATTTTCGCGCTCCGAAATAATCTGTAGGCTATGGTGAAGCACTTCAATACGTgtcg                    >  1:718599/1‑140 (MQ=255)
                                                                                                                             cgTTGGCCTGCTTTATTTGATCAATGTGCCCAGGCTGGTTTTCAGCAACCACCGCATACTCCCTATATTTTCGCGCTCCGAAATAATCTGTAGGCTATGGTGAAGCACTTCAATACGTGTCGTCAAATTTTTACTTAGGc  <  2:904926/140‑1 (MQ=255)
                                                                                                                             cgTTGGCCTGCTTTATTTGATCAATGTGCCCAGGCTGGTTTTCAGCAACCACCGCATACTCCCTATATTTTCGCGCTCCGAAATAATCTGTAGGCTATGGTGAAGCACTTCAATACGTGTCGTCAAATTTTTACTTAGGc  <  1:600592/140‑1 (MQ=255)
                                                                                                                             cgTTGGCCTGCTTTATTTGATCAATGTGCCCAGGCTGGTTTTCAGCAACCACCGCATACTCCCTATATTTTCGCGCTCCGAAATAATCTGTAGGCTATGGTGAAGCACTTCAATACGTGTCGTCAAATTTTTACTTAGGc  <  2:9749/140‑1 (MQ=255)
                                                                                                                                   |                                                                                                                                     
CAGGTGTGCTTCGAGCATCTCACGGACAATTTTAGTGATACTGGCAGGAGCCAGTTGCGCCAGACGGGAAAGATCGATACGCGAGACTGGACCAAGCTGATCAATCAGGCGATAAACCGCGCCCGCGTTGGTCTGCTTTATTTGATCAATGTGCCCAGGCTGGTTTTCAGCAACCACCGCATACTCCCTATATTTTCGCGCTCCGAAATAATCTGTAGGCTATGGTGAAGCACTTCAATACGTGTCGTCAAATTTTTACTTAGGC  >  NC_000913/1668388‑1668652

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑