Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,709,822	G→A	W227* (TGG→TGA)	rsxD →	SoxR [2Fe‑2S] reducing system protein RsxD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,709,822	0	G	A	100.0%	172.1 / NA	49	W227* (TGG→TGA)	rsxD	SoxR [2Fe‑2S] reducing system protein RsxD
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (23/26);  total (23/26)

GGCATTAGTCAGGCGACACCGCTGGATACATTTAAAACCTCTGTCCGTGCCGGTCATTCGGTTGAACAGATTATGCAATATCCGATCTACAGCGGTATTCTGGCGGGCGCTGGTTGGCAATGGGTAAATCTCGCCTGGCTGGCTGGCGGCGTATGGTTGCTATGGCAGAAAGCGATTCGCTGGCATATTCCCCTCAGCTTCTTAGTAACGCTGGCGTTATGCGCAATGTTGGGCTGGTTGTTCTCACCAGAAACACTGGCAGCACCGCAAATTC  >  NC_000913/1709685‑1709958                                                                                                                                          |                                                                                                                                         ggCATTAGTCAGGCGACACCGCTGGATACATTTAAAACCTCTGTCCGTGCCGGTCATTCGGTTGAACAGATTATGCAATATCCGATCTACAGCGGTATTCTGGCGGGCGCTGGTTGGCAATGGGTAAATCTCGCctgact                                                                                                                                        >  1:205465/1‑140 (MQ=255)    aTTAGTCAGGCGACACCGCTGGATACATTTAAAACCTCTGTCCGTGCCGGTCATTCGGTTGAACAGATTATGCAATATCCGATCTACAGCTGTATTCTGGCGGGCGCTGGTTGGCAATGGGTAAATCTCGCctgactggc                                                                                                                                     >  1:367633/1‑140 (MQ=255)       aGTCAGGCGACACCGCTGGATACATTTAAAACCTCTGTCCGTGCCGGTCATTCGGTTGAACAGATTATGCAATATCCGATCTACAGCGGTATTCTGGCGGGCGCTGGTTGGCAATGGGTAAATCTCGCctgactggctgg                                                                                                                                  <  1:446974/140‑1 (MQ=255)       aGTCAGGCGACACCGCTGGATACATTTAAAACCTCTGTCCGTGCCGGTCATTCGGTTGAACAGATTATGCAATATCCGATCTACAGCGGTATTCTGGCGGGCGCTGGTTGGCAATGGGTAAATCTCGCctgactggctgg                                                                                                                                  <  2:205465/140‑1 (MQ=255)          cAGGCGACACCGCTGGATACATTTAAAACCTCTGTCCGTGCCGGTCATTCGGTTGAACAGATTATGCAATATCCGATCTACAGCGGTATTCTGGCGGGCGCTGGTTGGCAATGGGTAAATCTCGCCTGACTGGCTggcgg                                                                                                                               <  2:727151/140‑1 (MQ=255)            ggCGACACCGCTGGATACATTTAAAACCTCTGTCCGTGCCGGTCATTCGGTTGAACAGATTATGCAATATCCGATCTACAGCGGTATTCTGGCGGGCGCTGGTTGGCAATGGGTAAATCTCGCCTGACTGGCTGgcggcg                                                                                                                             <  2:879865/140‑1 (MQ=255)             gCGACACCGCTGGATACATTTAAAACCTCTGTCCGTGCCGGTCATTCGGTTGAACAGATTATGCAATATCCGATCTACAGCGGTATTCTGGCGGGCGCTGGTTGGCAATGGGTAAATCTCGCCTGACTGGCTGgcggcgg                                                                                                                            >  1:515194/1‑139 (MQ=255)             gCGACACCGCTGGATACATTTAAAACCTCTGTCCGTGCCGGTCATTCGGTTGAACAGATTATGCAATATCCGATCTACAGCGGTATTCTGGCGGGCGCTGGTTGGCAATGGGTAAATCTCGCCTGACTGGCTGGCGGCGt                                                                                                                            >  1:944059/1‑140 (MQ=255)                acacCGCTGGATACATTTAAAACCTCTGTCCGTGCCGGTCATTCGGTTGAACAGATTATGCAATATCCGATCTACAGCGGTATTCTGGCGGGCGCTGGTTGGCAATGGGTAAATCTCGCctgactggctgg                                                                                                                                  <  2:635784/131‑1 (MQ=255)                acacCGCTGGATACATTTAAAACCTCTGTCCGTGCCGGTCATTCGGTTGAACAGATTATGCAATATCCGATCTACAGCGGTATTCTGGCGGGCGCTGGTTGGCAATGGGTAAATCTCGCctgactggctgg                                                                                                                                  >  1:635784/1‑131 (MQ=255)                acacCGCTGGATACATTTAAAACCTCTGTCCGTGCCGGTCATTCGGTTGAACAGATTATGCAATATCCGATCTACAGCGGTATTCTGGCGGGCGCTGGTTGGCAATGGGTAAATCTCGCCTGACTGGCTGGCGGCGTATg                                                                                                                         <  1:236036/140‑1 (MQ=255)                  acCGCTGGATACATTTAAAACCTCTGTCCGTGCCGGTCATTCGGTTGAACAGATTATGCAATATCCGATCTACAGCGGTATTCTGGCGGGCGCTGGTTGGCAATGGGTAAATCTCGCCTGACTGGCTGGCGGCGTATGGt                                                                                                                       >  1:294924/1‑140 (MQ=255)                  acCGCTGGATACATTTAAAACCTCTGTCCGTGCCGGTCATTCGGTTGAACAGATTATGCAATATCCGATCTACAGCGGTATTCTGGCGGGCGCTGGTTGGCAATGGGTAAATCTCGCCTGACTGGCTGGCGGCGTATGGt                                                                                                                       >  2:432439/1‑140 (MQ=255)                  acCGCTGGATACATTTAAAACCTCTGTCCGTGCCGGTCATTCGGTTGAACAGATTATGCAATATCCGATCTACAGCGGTATTCTGGCGGGCGCTGGTTGGCAATGGGTAAATCTCGCCTGACTGGCTGGCGGCGTATGGt                                                                                                                       <  2:944059/140‑1 (MQ=255)                   ccGCTGGATACATTTAAAACCTCTGTCCGTGCCGGTCATTCGGTTGAACAGATTATGCAATATCCGATCTACAGCGGTATTCTGGCGGGCGCTGGTTGGCAATGGGTAAATCTCGCCTGACTGGCTGGCGGCGTATggat                                                                                                                      >  1:118564/1‑138 (MQ=255)                       tGGATACATTTAAAACCTCTGTCCGTGCCGGTCATTCGGTTGAACAGATTATGCAATATCCGATCTACAGCGGTATTCTGGCGGGCGCTGGTTGGCAATGGGTAAATCTCGCCTGACTGGCTGGCGGCGTATGGTTGCTa                                                                                                                  <  1:450869/140‑1 (MQ=255)                                ttAAAACCTCTGTCCGTGCCGGTCATTCGGTTGAACAGATTATGCAATATCCGATCTACAGCGGTATTCTGGCGGGCGCTGGTTGGCAATGGGTAAATCTCGCCTGACTGGCTGGCGGCGTATGGTTGCTATGGCAGaaa                                                                                                         >  2:273820/1‑140 (MQ=255)                                   aaaCCTCTGTCCGTGCCGGTCATTCGGTTGAACAGATTATGCAATATCCGATCTACAGCGGTATTCTGGCGGGCGCTGGTTGGCAATGGGTAAATCTCGCCTGACTGGCTGGCGGCGTATGGTTGCTATGGCAGAAAGCg                                                                                                      >  1:605800/1‑140 (MQ=255)                                       ctctGTCCGTGCCGGTCATTCGGTTGAACAGATTATGCAATATCCGATCTACAGCGGTATTCTGGCGGGCGCTGGTTGGCAATGGGTAAATCTCGCCTGACTGGCTGGCGGCGTATGGTTGCTATGGCAGAAAGCGATTc                                                                                                  >  2:40367/1‑140 (MQ=255)                                        tctGTCCGTGCCGGTCATTCGGTTGAACAGATTATGCAATATCCGATCTACAGCGGTATTCTGGCGGGCGCTGGTTGGCAATGGGTAAATCTCGCCTGACTGGCTGGCGGCGTATGGTTGCTATGGCAGAAAGCGATTCg                                                                                                 <  1:431304/140‑1 (MQ=255)                                        tctGTCCGTGCCGGTCATTCGGTTGAACAGATTATGCAATATCCGATCTACAGCGGTATTCTGGCGGGCGCTGGTTGGCAATGGGTAAATCTCGCCTGACTGGCTGGCGGCGTATGGTTGCTATGGCAGAAAGCGATTCg                                                                                                 <  2:545717/140‑1 (MQ=255)                                        tctGTCCGTGCCGGTCATTCGGTTGAACAGATTATGCAATATCCGATCTACAGCGGTATTCTGGCGGGCGCTGGTTGGCAATGGGTAAATCTCGCCTGACTGGCTGGCGGCGTATGGTTGCTATGGCAGAAAGCGATTCg                                                                                                 <  1:613582/140‑1 (MQ=255)                                        tctGTCCGTGCCGGTCATTCGGTTGAACAGATTATGCAATATCCGATCTACAGCGGTATTCTGGCGGGCGCTGGTTGGCAATGGGTAAATCTCGCCTGACTGGCTGGCGGCGTATGGTTGCTATGGCAGAAAGCGATTCg                                                                                                 <  1:273820/140‑1 (MQ=255)                                        tctGTCCGTGCCGGTCATTCGGTTGAACAGATTATGCAATATCCGATCTACAGCGGTATTCTGGCGGGCGCTGGTTGGCAATGGGTAAATCTCGCCTGACTGGCTGGCGGCGTATGGTTGCTATGGCAGAAAGCGATTCg                                                                                                 >  1:150720/1‑140 (MQ=255)                                         ctGTCCGTGCCGGTCATTCGGTTGAACAGATTATGCAATATCCGATCTACAGCGGTATTCTGGCGGGCGCTGGTTGGCAATGGGTAAATCTCGCCTGACTGGCTGGCGGCGTATGGTTGCTATGGCAGAAAGCGATTCGc                                                                                                >  1:162664/1‑140 (MQ=255)                                         ctGTCCGTGCCGGTCATTCGGTTGAACAGATTATGCAATATCCGATCTACAGCGGTATTCTGGCGGGCGCTGGTTGGCAATGGGTAAATCTCGCCTGACTGGCTGGCGGCGTATGGTTGCTATGGCAGAAAGCGATTCGc                                                                                                >  2:12068/1‑140 (MQ=255)                                          tGTCCGTGCCGGTCATTCGGTTGAACAGATTATGCAATATCCGATCTACAGCGGTATTCTGGCGGGCGCTGGTTGGCAATGGGTAAATCTCGCCTGACTGGCTGGCGGCGTATGGTTGCTATGGCAGAAAGCGATTCGCt                                                                                               <  2:294924/140‑1 (MQ=255)                                           gTCCGTGCCGGTCATTCGGTTGAACAGATTATGCAATATCCGATCTACAGCGGTATTCTGGCGGGCGCTGGTTGGCAATGGGTAAATCTCGCCTGACTGGCTGGCGGCGTATGGTTGCTATGGCAGAAAGCGATTCGCTg                                                                                              <  1:767139/140‑1 (MQ=255)                                           gTCCGTGCCGGTCATTCGGTTGAACAGATTATGCAATATCCGATCTACAGCGGTATTCTGGCGGGCGCTGGTTGGCAATGGGTAAATCTCGCCTGACTGGCTGGCGGCGTATGGTTGCTATGGCAGAAAGCGATTCGCTg                                                                                              <  2:808463/140‑1 (MQ=255)                                                        aTTCGGTTGAACAGATTATGCAATATCCGATCTACAGCGGTATTCTGGCGGGCGCTGGTTGGCAATGGGTAAATCTCGCCTGACTGGCTGGCGGCGTATGGTTGCTATGGCAGAAAGCGATTCGCTGGCATATTCCCCTc                                                                                 <  2:188244/140‑1 (MQ=255)                                                          tCGGTTGAACAGATTATGCAATATCCGATCTACAGCGGTATTCTGGCGGGCGCTGGTTGGCAATGGGTAAATCTCGCCTGACTGGCTGGCGGCGTATGGTTGCTATGGCAGAAAGCGATTCGCTGGCATATTCCCCTCAg                                                                               <  1:432439/140‑1 (MQ=255)                                                          tCGGTTGAACAGATTATGCAATATCCGATCTACAGCGGTATTCTGGCGGGCGCTGGTTGGCAATGGGTAAATCTCGCCTGACTGGCTGGCGGCGTATGGTTGCTATGGCAGAAAGCGATTCGCTGGCATATTCCCCTCAg                                                                               <  2:150720/140‑1 (MQ=255)                                                            ggTTGAACAGATTATGCAATATCCGATCTACAGCGGTATTCTGGCGGGCGCTGGTTGGCAATGGGTAAATCTCGCCTGACTGGCTGGCGGCGTATGGTTGCTATGGCAGAAAGCGATTCGCTGGCATATTCCCCTCAGct                                                                             <  2:605800/140‑1 (MQ=255)                                                                   cAGATTATGCAATATCCGATCTACAGCTGTATTCTGGCGGGCGCTGGTTGGCAATGGGTAAATCTCGCCTGACTGGCTGGCGGCGTATGGTTGCTATGGCAGAAAGCGATTCGCTGGCATATTCCCCTCAGCTTCTTAGt                                                                      <  2:367633/140‑1 (MQ=255)                                                                   cAGATTATGCAATATCCGATCTACAGCGGTATTCTGGCGGGCGCTGGTTGGCAATGGGTAAATCTCGCCTGACTGGCTGGCGGCGTATGGTTGCTATGGCAGAAAGCGATTCGCTGGCATATTCCCCTCAGCTTCTTAGt                                                                      <  1:40367/140‑1 (MQ=255)                                                                        tATGCAATATCCGATCTACAGCGGTATTCTGGCGGGCGCTGGTTGGCAATGGGTAAATCTCGCCTGACTGGCTGGCGGCGTATGGTTGCTATGGCAGAAAGCGATTCGCTGGCATATTCCCCTCAGCTTCTTAg                                                                       <  2:283841/134‑1 (MQ=255)                                                                        tATGCAATATCCGATCTACAGCGGTATTCTGGCGGGCGCTGGTTGGCAATGGGTAAATCTCGCCTGACTGGCTGGCGGCGTATGGTTGCTATGGCAGAAAGCGATTCGCTGGCATATTCCCCTCAGCTTCTTAg                                                                       >  1:283841/1‑134 (MQ=255)                                                                             aaTATCCGATCTACAGCGGTATTCTGGCGGGCGCTGGTTGGCAATGGGTAAATCTCGCCTGACTGGCTGGCGGCGTATGGTTGCTATGGCAGAAAGCGATTCGCTGGCATATTCCCCTCAGCTTCTTAGTAACGCTGGCg                                                            <  2:491413/140‑1 (MQ=255)                                                                               tatCCGATCTACAGCGGTATTCTGGCGGGCGCTGGTTGGCAATGGGTAAATCTCGCCTGACTGGCTGGCGGCGTATGGTTGCTATGGCAGAAAGCGATTCGCTGGCATATTCCCCTCAGCTTCTCAGTAACGCTGGCGtt                                                          >  2:93972/1‑140 (MQ=255)                                                                                atCCGATCTACAGCGGTATTCTGGCGGGCGCTGGTTGGCAATGGGTAAATCTCGCCTGACTGGCTGGCGGCGTATGGTTGCTATGGCAGAAAGCGATTCGCTGGCATATTCCCCTCAGCTTCTTAGTAACGCTGGCGTTa                                                         >  2:195541/1‑140 (MQ=255)                                                                                atCCGATCTACAGCGGTATTCTGGCGGGCGCTGGTTGGCAATGGGTAAATCTCGCCTGACTGGCTGGCGGCGTATGGTTGCTATGGCAGAAAGCGATTCGCTGGCATATTCCCCTCAGCTTCTTAGTAACGCTGGCGTTa                                                         >  1:122351/1‑140 (MQ=255)                                                                                 tCCGATCTACAGCGGTATTCTGGCGGGCGCTGGTTGGCAATGGGTAAATCTCGCCTGACTGGCTGGCGGCGTATGGTTGCTATGGCAGAAAGCGATTCGCTGGCATATTCCCCTCAGCTTCTTAGTAACGCTGGCGTTAt                                                        >  2:248510/1‑140 (MQ=255)                                                                                             cGGTATTCTGGCGGGCGCTGGTTGGCAATGGGTAAATCTCGCCTGACTGGCTGGCGGCGTATGGTTGCTATGGCAGAAAGCGATTCGCTGGCATATTCCCCTCAGCTTCTTAGTAACGCTGGCGTTATGCGCAATGTTgg                                            >  1:710362/1‑140 (MQ=255)                                                                                              ggTATTCTGGCGGGCGCTGGTTGGCAATGGGTAAATCTCGCCTGACTGGCTGGCGGCGTATGGTTGCTATGGCAGAAAGCGATTCGCTGGCATATTCCCCTCAGCTTCTTAGTAACGCTGGCGTTATGCGCAATGTTggg                                           <  1:195541/140‑1 (MQ=255)                                                                                               gTATTCTGGCGGGCGCTGGTTGGCAATGGGTAAATCTCGCCTGACTGGCTGGCGGCGTATGGTTGCTATGGCAGAAAGCGATTCGCTGGCATATTCCCCTCAGCTTCTTAGTAACGCTGGCGTTATGCGCAATGTTGGGc                                          >  1:924751/1‑140 (MQ=255)                                                                                               gTATTCTGGCGGGCGCTGGTTGGCAATGGGTAAATCTCGCCTGACTGGCTGGCGGCGTATGGTTGCTATGGCAGAAAGCGATTCGCTGGCATATTCCCCTCAGCTTCTTAGTAACGCTGGCGTTATGCGCAATGTTGGGc                                          <  1:12068/140‑1 (MQ=255)                                                                                                tATTCTGGCGGGCGCTGGTTGGCAATGGGTAAATCTCGCCTGACTGGCTGGCGGCGTATGGTTGCTATGGCAGAAAGCGATTCGCTGGCATATTCCCCTCAGCTTCTTAGTATCGCTGGCGTTATGCGCAATGTTGGGCt                                         >  1:223823/1‑140 (MQ=255)                                                                                                tATTCTGGCGGGCGCTGGTTGGCAATGGGTAAATCTCGCCTGACTGGCTGGCGGCGTATGGTTGCTATGGCAGAAAGCGATTCGCTGGCATATTCCCCTCAGCTTCTTAGTAACGCTGGCGTTATGCGCAATGTTgg                                            >  2:15007/1‑137 (MQ=255)                                                                                                tATTCTGGCGGGCGCTGGTTGGCAATGGGTAAATCTCGCCTGACTGGCTGGCGGCGTATGGTTGCTATGGCAGAAAGCGATTCGCTGGCATATTCCCCTCAGCTTCTTAGTAACGCTGGCGTTATGCGCAATGTTgg                                            <  1:15007/137‑1 (MQ=255)                                                                                                  ttCTGGCGGGCGCTGGTTGGCAATGGGTAAATCTCGCCTGACTGGCTGGCGGCGTATGGTTGCTATGGCAGAAAGCGATTCGCTGGCATATTCCCCTCAGCTTCTTAGTAACGCTGGCGTTATgcg                                                     >  1:837189/1‑126 (MQ=255)                                                                                                  ttCTGGCGGGCGCTGGTTGGCAATGGGTAAATCTCGCCTGACTGGCTGGCGGCGTATGGTTGCTATGGCAGAAAGCGATTCGCTGGCATATTCCCCTCAGCTTCTTAGTAACGCTGGCGTTATgcg                                                     <  2:837189/126‑1 (MQ=255)                                                                                                                           ggTAAATCTCGCCTGACTGGCTGGCGGCGTATGGTTGCTATGGCAGAAAGCGATTCGCTGGCATATTCCCCTCAGCTTCTTAGTAACGCTGGCGTTATGCGCAATGTTGGGCTGGTTGTTCTCACCAGAAACACTGgcag              <  2:515194/140‑1 (MQ=255)                                                                                                                            gTAAATCTCGCCTGACTGGCTGGCGGCGTATGGTTGCTATGGCAGAAAGCGATTCGCTGGCATATTCCCCTCAGCTTCTTAGTAACGCTGGCGTTATGCGCAATGTTGGGCTGGTTGTTCTCACCAGAAACACTGgcagc             >  1:141824/1‑140 (MQ=255)                                                                                                                                  ctcGCCTGACTGGCTGGCGGCGTATGGTTGCTATGGCAGAAAGCGATTCGCTGGCATATTCCCCTCAGCTTCTTAGTAACGCTGGCGTTATGCGCAATGTTGGGCTGGTTGTTCTCACCAGAAACACTGGCAGCACCGCa       <  2:534097/140‑1 (MQ=255)                                                                                                                                      ccTGACTGGCTGGCGGCGTATGGTTGCTATGGCAGAAAGCGATTCGCTGGCATATTCCCCTCAGCTTCTTAGTAACGCTGGCGTTATGCGCAATGTTGGGCTGGTTGTTCTCACCAGAAACACTGGCAGCACCGCAAAtt   >  1:345732/1‑140 (MQ=255)                                                                                                                                       ctgactggctggCGGCGTATGGTTGCTATGGCAGAAAGCGATTCGCTGGCATATTCCCCTCAGCTTCTTAGTATCGCTGGCGTTATGCGCAATGTTGGGCTGGTTGTTCTCACCAGAAACACTGGCAGCACCGCAAATTc  <  2:223823/140‑1 (MQ=255)                                                                                                                                          |                                                                                                                                         GGCATTAGTCAGGCGACACCGCTGGATACATTTAAAACCTCTGTCCGTGCCGGTCATTCGGTTGAACAGATTATGCAATATCCGATCTACAGCGGTATTCTGGCGGGCGCTGGTTGGCAATGGGTAAATCTCGCCTGGCTGGCTGGCGGCGTATGGTTGCTATGGCAGAAAGCGATTCGCTGGCATATTCCCCTCAGCTTCTTAGTAACGCTGGCGTTATGCGCAATGTTGGGCTGGTTGTTCTCACCAGAAACACTGGCAGCACCGCAAATTC  >  NC_000913/1709685‑1709958

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑