Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,787,733	G→A	V97I (GTT→ATT)	ppsR →	posphoenolpyruvate synthetase regulatory protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,787,733	0	G	A	100.0%	192.8 / NA	55	V97I (GTT→ATT)	ppsR	posphoenolpyruvate synthetase regulatory protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (27/28);  total (27/28)

TGAAGGATCAGATTGACGCAATTTATCACCAGACAGGCGTGCGCCCGCTGGTCTTCTACTCCATCGTGTTGCCGGAGATTCGCGCCATCATCTTGCAAAGTGAAGGCTTTTGCCAGGATATCGTTCAGGCGCTGGTTGCCCCGCTACAACAAGAGATGAAACTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATAATCTCAATAAATATGATGCGCGCATTGCGGCGATTGATTACACCCTCGCCCACGATG  >  NC_000913/1787599‑1787865                                                                                                                                       |                                                                                                                                     tGAAGGATCAGATTGACGCAATTTATCACCAGACAGGCGTGCGCCCGCTGGTCTTCTACTCCATCGTGTTGCCGGAGATTCGCGCCATCATCTTGCAAAGTGAAGGCTTTTGCCAGGATATCGTTCAGGCGCTGATTGcc                                                                                                                                 >  1:131084/1‑140 (MQ=255) tGAAGGATCAGATTGACGCAATTTATCACCAGACAGGCGTGCGCCCGCTGGTCTTCTACTCCATCGTGTTGCCGGAGATTCGCGCCATCATCTTGCAAAGTGAAGGCTTTTGCCAGGATATCGTTCAGGCGCTGATTGcc                                                                                                                                 >  1:76522/1‑140 (MQ=255)  gAAGGATCAGATTGACGCAATTTATCACCAGACAGGCGTGCGCCCGCTGGTCTTCTACTCCATCGTGTTGCCGGAGATTCGCGCCATCATCTTGCAAAGTGAAGGCTTTTGCCAGGATATCGTTCAGGCGCTGATTGccc                                                                                                                                <  1:361229/140‑1 (MQ=255)   aaGGATCAGATTGACGCAATTTATCACCAGACAGGCGTGCGCCCGCTGGTCTTCTACTCCATCGTGTTGCCGGAGATTCGCGCCATCATCTTGCAAAGTGAAGGCTTTTGCCAGGATATCGTTCAGGCGCTGATTGcccc                                                                                                                               <  1:918865/140‑1 (MQ=255)       aTCAGATTGACGCAATTTATCACCAGACAGGCGTGCGCCCGCTGGTCTTCTACTCCATCGTGTTGCCGGAGATTCGCGCCATCATCTTGCAAAGTGAAGGCTTTTGCCAGGATATCGTTCAGGCGCTGATTGCCCCGCTa                                                                                                                           >  1:197586/1‑140 (MQ=255)           gATTGACGCAATTTATCACCAGACAGGCGTGCGCCCGCTGGTCTTCTACTCCATCGTGTTGCCGGAGATTCGCGCCATCATCTTGCAAAGTGAAGGCTTTTGCCAGGATATCGTTCAGGCGCTGATTGCCCCGCTacaac                                                                                                                       >  1:71892/1‑140 (MQ=255)           gATTGACGCAATTTATCACCAGACAGGCGTGCGCCCGCTGGTCTTCTACTCCATCGTGTTGCCGGAGATTCGCGCCATCATCTTGCAAAGTGAAGGCTTTTGCCAGGATATCGTTCAGGCGCTGATTGCCCCGCTacaac                                                                                                                       >  2:128076/1‑140 (MQ=255)                aCGCAATTTATCACCAGACAGGCGTGCGCCCGCTGGTCTTCTACTCCATCGTGTTGCCGGAGATTCGCGCCATCATCTTGCAAAGTGAAGGCTTTTGCCAGGATATCGTTCAGGCGCTGATTGCCCCGCTacaaca                                                                                                                      >  2:344939/1‑136 (MQ=255)                aCGCAATTTATCACCAGACAGGCGTGCGCCCGCTGGTCTTCTACTCCATCGTGTTGCCGGAGATTCGCGCCATCATCTTGCAAAGTGAAGGCTTTTGCCAGGATATCGTTCAGGCGCTGATTGCCCCGCTacaaca                                                                                                                      <  1:344939/136‑1 (MQ=255)                 cGCAATTTATCACCAGACAGGCGTGCGCCCGCTGGTCTTCTACTCCATCGTGTTGCCGGAGATTCGCGCCATCATCTTGCAAAGTGAAGGCTTTTGCCAGGATATCGTTCAGGCGCTGATTGCCCCGCTACAACAAgaga                                                                                                                 <  2:82142/140‑1 (MQ=255)                                        tGCGCCCGCTGGTCTTCTACTCCATCGTGTTGCCGGAGATTCGCGCCATCATCTTGCAAAGTGAAGGCTTTTGCCAGGATATCGTTCAGGCGCTGATTGCCCCGCTACAACAAGAGATGAAACTGGATCCAACGCCGAtt                                                                                          >  1:912467/1‑140 (MQ=255)                                         gcgcCCGCTGGTCTTCTACTCCATCGTGTTGCCGGAGATTCGCGCCATCATCTTGCAAAGTGAAGGCTTTTGCCAGGATATCGTTCAGGCGCTGATTGCCCCGCTACAACAAGAGATGAAACTGGATCCAACGCCGATTg                                                                                         <  1:47487/140‑1 (MQ=255)                                              cGCTGGTCTTCTACTCCATCGTGTTGCCGGAGATTCGCGCCATCATCTTGCAAAGTGAAGGCTTTTGCCAGGATATCGTTCAGGCGCTGATTGCCCCGCTACAACAAGAGATGAAACTGGATCCAACGCCGATTGCTCAt                                                                                    <  2:131084/140‑1 (MQ=255)                                              cGCTGGTCTTCTACTCCATCGTGTTGCCGGAGATTCGCGCCATCATCTTGCAAAGTGAAGGCTTTTGCCAGGATATCGTTCAGGCGCTGATTGCCCCGCTACAACAAGAGATGAAACTGGATCCAACGCCGATTGCTCAt                                                                                    <  2:76522/140‑1 (MQ=255)                                               gCTGGTCTTCTACTCCATCGTGTTGCCGTAGATTCGCGCCATCATCTTGCAAAGTGAAGGCTTTTGCCAGGATATCGTTCAGGCGCTGATTGCCCCGCTACAACAAGAGATGAAACTGGATCCAACGCCGATTGCTCATc                                                                                   >  2:294179/1‑140 (MQ=255)                                               gCTGGTCTTCTACTCCATCGTGTTGCCGGAGATTCGCGCCATCATCTTGCAAAGTGAAGGCTTTTGCCAGGATATCGTTCAGGCGCTGATTGCCCCGCTACAACAAGAGATGAAACTGGATCCAACGCCGATTGCTCATc                                                                                   >  2:470058/1‑140 (MQ=255)                                               gCTGGTCTTCTACTCCATCGTGTTGCCGGAGATTCGCGCCATCATCTTGCAAAGTGAAGGCTTTTGCCAGGATATCGTTCAGGCGCTGATTGCCCCGCTACAACAAGAGATGAAACTGGATCCAACGCCGATTGCTCATc                                                                                   >  1:124274/1‑140 (MQ=255)                                               gCTGGTCTTCTACTCCATCGTGTTGCCGGAGATTCGCGCCATCATCTTGCAAAGTGAAGGCTTTTGCCAGGATATCGTTCAGGCGCTGATTGCCCCGCTACAACAAGAGATGAAACTGGATCCAACGCCGATTGCTCATc                                                                                   >  1:30294/1‑140 (MQ=255)                                                cTGGTCTTCTACTCCATCGTGTTGCCGTAGATTCGCGCCATCATCTTGCAAAGTGAAGGCTTTTGCCAGGATATCGTTCAGGCGCTGATTGCCCCGCTACAACAAGAGATGAAACTGGATCCAACGCCGATTGCTCATCg                                                                                  <  1:294179/140‑1 (MQ=255)                                                cTGGTCTTCTACTCCATCGTGTTGCCGGAGATTCGCGCCATCATCTTGCAAAGTGAAGGCTTTTGCCAGGATATCGTTCAGGCGCTGATTGCCCCGCTACAACAAGAGATGAAACTGGATCCAACGCCGATTGCTCATCg                                                                                  <  1:128076/140‑1 (MQ=255)                                                           cTCCATCGTGTTGCCGGAGATTCGCGCCATCATCTTGCAAAGTGAAGGCTTTTGCCAGGATATCGTTCAGGCGCTGATTGCCCCGCTACAACAAGAGATGAAACTGGATCCAACGCCGATTGCTCATCg                                                                                  >  2:95432/1‑129 (MQ=255)                                                           cTCCATCGTGTTGCCGGAGATTCGCGCCATCATCTTGCAAAGTGAAGGCTTTTGCCAGGATATCGTTCAGGCGCTGATTGCCCCGCTACAACAAGAGATGAAACTGGATCCAACGCCGATTGCTCATCg                                                                                  <  1:95432/129‑1 (MQ=255)                                                            tCCATCGTGTTGCCGGAGATTCGCGCCATCATCTTGCAAAGTGAAGGCTTTTGCCAGGATATCGTTCAGGCGCTGATTGCCCCGCTACAACAAGAGATGAAACTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCt                                                                      <  1:470058/140‑1 (MQ=255)                                                            tCCATCGTGTTGCCGGAGATTCGCGCCATCATCTTGCAAAGTGAAGGCTTTTGCCAGGATATCGTTCAGGCGCTGATTGCCCCGCTACAACAAGAGATGAAACTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCt                                                                      <  2:912467/140‑1 (MQ=255)                                                             ccATCGTGTTGCCGGAGATTCGCGCCATCATCTTGCAAAGTGAAGGCTTTTGCCAGGATATCGTTCAGGCGCTGATTGCCCCGCTACAACAAGAGATGAAACTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCtt                                                                     >  2:30616/1‑140 (MQ=255)                                                             ccATCGTGTTGCCGGAGATTCGCGCCATCATCTTGCAAAGTGAAGGCTTTTGCCAGGATATCGTTCAGGCGCTGATTGCCCCGCTACAACAAGAGATGAAACTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCtt                                                                     >  2:515035/1‑140 (MQ=255)                                                              cATCGTGTTGCCGGAGATTCGCGCCATCATCTTGCAAAGTGAAGGCTTTTGCCAGGATATCGTTCAGGCGCTGATTGCCCCGCTACAACAAGAGATGAAACTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTa                                                                    >  1:421160/1‑140 (MQ=255)                                                              cATCGTGTTGCCGGAGATTCGCGCCATCATCTTGCAAAGTGAAGGCTTTTGCCAGGATATCGTTCAGGCGCTGATTGCCCCGCTACAACAAGAGATGAAACTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTa                                                                    >  2:175533/1‑140 (MQ=255)                                                               aTCGTGTTGCCGGAGATTCGCGCCATCATCTTGCAAAGTGAAGGCTTTTGCCAGGATATCGTTCAGGCGCTGATTGCCCCGCTACAACAAGAGATGAAACTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTaa                                                                   <  2:417152/140‑1 (MQ=255)                                                                tCGTGTTGCCGGAGATTCGCGCCATCATCTTGCAAAGTGAAGGCTTTTGCCAGGATATCGTTCAGGCGCTGATTGCCCCGCTACAACAAGAGATGAAACTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAAc                                                                  >  1:362497/1‑140 (MQ=255)                                                                tCGTGTTGCCGGAGATTCGCGCCATCATCTTGCAAAGTGAAGGCTTTTGCCAGGATATCGTTCAGGCGCTGATTGCCCCGCTACAACAAGAGATGAAACTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAAc                                                                  <  1:30616/140‑1 (MQ=255)                                                                 cGTGTTGCCGGAGATTCGCGCCATCATCTTGCAAAGTGAAGGCTTTTGCCAGGATATCGTTCAGGCGCTGATTGCCCCGCTACAACAAGAGATGAAACTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAAcc                                                                 >  1:710638/1‑140 (MQ=255)                                                                  gtgtTGCCGGAGATTCGCGCCATCATCTTGCAAAGTGAAGGCTTTTGCCAGGATATCGTTCAGGCGCTGATTGCCCCGCTACAACAAGAGATGAAACTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAAccc                                                                >  1:673250/1‑140 (MQ=255)                                                                   tgtTGCCGGAGATTCGCGCCATCATCTTGCAAAGTGAAGGCTTTTGCCAGGATATCGTTCAGGCGCTGATTGCCCCGCTACAACAAGAGATGAAACTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCt                                                               >  2:169954/1‑140 (MQ=255)                                                                   tgtTGCCGGAGATTCGCGCCATCATCTTGCAAAGTGAAGGCTTTTGCCAGGATATCGTTCAGGCGCTGATTGCCCCGCTACAACAAGAGATGAAACTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCt                                                               >  2:67008/1‑140 (MQ=255)                                                                     ttGCCGGAGATTCGCGCCATCATCTTGCAAAGTGAAGGCTTTTGCCAGGATATCGTTCAGGCGCTGATTGCCCCGCTACAACAAGAGATGAAACTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCtaa                                                             >  2:93433/1‑140 (MQ=255)                                                                       gCCGGAGATTCGCGCCATCATCTTGCAAAGTGAAGGCTTTTGCCAGGATATCGTTCAGGCGCTGATTGCCCCGCTACAACAAGAGATGAAACTGGATCCAACGCCGATCGCTCATCGTACCCATGGCCTTAACCCtaata                                                           <  2:684122/140‑1 (MQ=255)                                                                        ccGGAGATTCGCGCCATCATCTTGCAAAGTGAAGGCTTTTGCCAGGATATCGTTCAGGCGCTGATTGCCCCGCTACAACAAGAGATGAAACTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCtaataa                                                          <  2:362497/140‑1 (MQ=255)                                                                         cGGAGATTCGCGCCATCATCTTGCAAAGTGAAGGCTTTTGCCAGGATATCGTTCAGGCGCTGATTGCCCCGCTACAACAAGAGATGAAACTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTaataat                                                         <  1:713102/140‑1 (MQ=255)                                                                         cGGAGATTCGCGCCATCATCTTGCAAAGTGAAGGCTTTTGCCAGGATATCGTTCAGGCGCTGATTGCCCCGCTACAACAAGAGATGAAACTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTaataat                                                         <  1:169954/140‑1 (MQ=255)                                                                         cGGAGATTCGCGCCATCATCTTGCAAAGTGAAGGCTTTTGCCAGGATATCGTTCAGGCGCTGATTGCCCCGCTACAACAAGAGATGAAACTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTaataat                                                         <  2:421160/140‑1 (MQ=255)                                                                         cGGAGATTCGCGCCATCATCTTGCAAAGTGAAGGCTTTTGCCAGGATATCGTTCAGGCGCTGATTGCCCCGCTACAACAAGAGATGAAACTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTaataat                                                         <  1:175533/140‑1 (MQ=255)                                                                         cGGAGATTCGCGCCATCATCTTGCAAAGTGAAGGCTTTTGCCAGGATATCGTTCAGGCGCTGATTGCCCCGCTACAACAAGAGATGAAACTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTaataat                                                         <  1:67008/140‑1 (MQ=255)                                                                            agaTTCGCGCCATCATCTTGCAAAGTGAAGGCTTTTGCCAGGATATCGTTCAGGCGCTGATTGCCCCGCTACAACAAGAGATGAAACTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATAAtctc                                                      <  2:710638/140‑1 (MQ=255)                                                                              aTTCGCGCCATCATCTTGCAAAGTGAAGGCTTTTGCCAGGATATCGTTCAGGCGCTGATTGCCCCGCTACAACAAGAGATGAAACTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATAATCTCaa                                                    <  2:197586/140‑1 (MQ=255)                                                                                   cgcCATCATCTTGCAAAGTGAAGGCTTTTGCCAGGATATCGTTCAGGCGCTGATTGCCCCGCTACAACAAGAGATGAAACTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTaataat                                                         <  2:66762/130‑1 (MQ=255)                                                                                   cgcCATCATCTTGCAAAGTGAAGGCTTTTGCCAGGATATCGTTCAGGCGCTGATTGCCCCGCTACAACAAGAGATGAAACTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTaataat                                                         >  1:66762/1‑130 (MQ=255)                                                                                                        aGGCTTTTGCCAGGATATCGTTCAGGCGCTGATTGCCCCGCTACAACAAGAGATGAAACTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATAATCTCAATAAATATGATGCGCGCATTGCGGCga                          <  2:673250/140‑1 (MQ=255)                                                                                                         ggCTTTTGCCAGGATATCGTTCAGGCGCTGATTGCTCCGCTACAACAAGAGATGAAACTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATAATCTCAATAAATATGATGCGCGCATTGCGGCgat                         <  1:114857/140‑1 (MQ=255)                                                                                                                      atatCGTTCAGGCGCTGATTGCCCCGCTACAACAAGAGATGAAACTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATAATCTCAATAAATATGATGCGCGCATTGCGGCGATTGATTACACCCTc            <  2:30294/140‑1 (MQ=255)                                                                                                                      atatCGTTCAGGCGCTGATTGCCCCGCTACAACAAGAGATGAAACTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATAATCTCAATAAATATGATGCGCGCATTGCGGCGATTGATTACACCCTc            >  1:868125/1‑140 (MQ=255)                                                                                                                        atCGTTCAGGCGCTGATTGCCCCGCTACAACAAGAGATGAAACTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATAATCTCAATAAATATGATGCGCGCATTGCGGCGATTGATTACACCCTCGc          >  1:696447/1‑140 (MQ=255)                                                                                                                            ttCAGGCGCTGATTGCCCCGCTACAACAAGAGATGAAACTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATAATCTCAATAAATATGATGCGCGCATTGCGGCGATTGATTACACCCTCGCCCAc      >  1:350278/1‑140 (MQ=255)                                                                                                                               aGGCGCTGATTGCCCCGCTACAACAAGAGATGAAACTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATAATCTCAATAAATATGATGCGCGCATTGCGGCGATTGATTACACCCTCGCCCACGAt   >  1:551463/1‑140 (MQ=255)                                                                                                                                ggCGCTGATTGCCCCGCTACAACAAGAGATGAAACTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATAATCTCAATAAATATGATGCGCGCATTGCGGCGATTGATTACACCCTCGCCCACGATg  <  2:258108/140‑1 (MQ=255)                                                                                                                                       |                                                                                                                                     TGAAGGATCAGATTGACGCAATTTATCACCAGACAGGCGTGCGCCCGCTGGTCTTCTACTCCATCGTGTTGCCGGAGATTCGCGCCATCATCTTGCAAAGTGAAGGCTTTTGCCAGGATATCGTTCAGGCGCTGGTTGCCCCGCTACAACAAGAGATGAAACTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATAATCTCAATAAATATGATGCGCGCATTGCGGCGATTGATTACACCCTCGCCCACGATG  >  NC_000913/1787599‑1787865

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑