Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,800,484	T→C	D52G (GAC→GGC)	infC ←	translation initiation factor IF‑3

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,800,484	0	T	C	100.0%	86.7 / NA	25	D52G (GAC→GGC)	infC	translation initiation factor IF‑3
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (13/12);  total (13/12)

GATAACTTTTTGCTTTTTCTTCTGTTCCTTAGAAGACTTGCTCTTTTCATAGAGGAATTTGCCGTAATCCATTATACGACAAACCGGCGGCTCGGCGTTAGGGCTGATCTCGACTAAGTCTACTCCGGCTTCTTCTGCTTTCTCCAGAGCTTCTCTCAGACTCACAATACCAAGCTGCTCGCCTTCCAGACCTGTTAAGCGAACTTCCTGGGCGCGAATTTCGCCATTGATACGGTTAGGGCGCGCCGTTTG  >  NC_000913/1800366‑1800617                                                                                                                       |                                                                                                                                      gATAACTTTTTGCTTTTTCTTCTGTTCCTTAGAAGACTTGCTCTTTTCATAGAGGAATTTGCCGTAATCCATTATACGACAAACCGGCGGCTCGGCGTTAGGGCTGATCTCGACTAAGCCTACTCCGGCTTCTTCTGCtt                                                                                                                  >  2:648607/1‑140 (MQ=255) gATAACTTTTTGCTTTTTCTTCTGTTCCTTAGAAGACTTGCTCTTTTCATAGAGGAATTTGCCGTAATCCATTATACGACAAACCGGCGGCTCGGCGTTAGGGCTGATCTCGACTAAGCCTACTCCGGCTTCTTCTGCtt                                                                                                                  >  2:567249/1‑140 (MQ=255)      cTTTTTGCTTTTTCTTCTGTTCCTTAGAAGACTTGCTCTTTTCATAGAGGAATCTGCCGTAATCCATTATACGACAAACCGGCGGCTCGGCGTTAGGGCTGATCTCGACTAAGCCTACTCCGGCTTCTTCTGCTTTCTcc                                                                                                             <  2:369954/140‑1 (MQ=255)       tttttGCTTTTTCTTCTGTTCCTTAGAAGACTTGCTCTTTTCATAGAGGAATTTGCCGTAATCCATTATACGACAAACCGGCGGCTCGGCGTTAGGGCTGATCTCGACTAAGCCTACTCCGGCTTCTTCTGCTTTCTCCa                                                                                                            >  2:530468/1‑140 (MQ=255)             cTTTTTCTTCTGTTCCTTAGAAGACTTGCTCTTTTCATAGAGGAATTTGCCGTAATCCATTATACGACAAACCGGCGGCTCGGCGTTAGGGCTGATCTCGACTAAGCCTACTCCGGCTTCTTCTGCTTTCTCCAGAGCtt                                                                                                      <  2:484980/140‑1 (MQ=255)                 ttcttcTGTTCCTTAGAAGACTTGCTCTTTTCATAGAGGAATTTGCCGTAATCCATTATACGACAAACCGGCGGCTCGGCGTTAGGGCTGATCTCGACTAAGCCTACTCCGGCTTCTTCTGCTTTCTCCAGAGCTtctct                                                                                                  <  1:648607/140‑1 (MQ=255)                            cTTAGAAGACTTGCTCTTTTCATAGAGGAATTTGCCGTAATCCATTATACGACAAACCGGCGGCTCGGCGTTAGGGCTGATCTCGACTAAGCCTACTCCGGCTTCTTCTGCTTTCTCCAGAGCTTCTCTCAGACTCACaa                                                                                       >  1:626575/1‑140 (MQ=255)                                   gaCTTGTTCTTTTCATAGAGGAATTTGCCGTAATCCATTATACGACAAACCGGCGGCTCGGCGTTAGGGCTGATCTCGACTAAGCCTACTCCGGCTTCTTCTGCTTTCTCCAGAGCTTCTCTCAGACTCACAATACCAAg                                                                                >  1:35885/1‑140 (MQ=255)                                        gCTCTTTTCATAGAGGAATTTGCCGTAATCCATTATACGACAAACCGGCGGCTCGGCGTTAGGGCTGATCTCGACTAAGCCTACTCCGGCTTCTTCTGCTTTCTCCAGAGCTTCTCTCAGACTca                                                                                          <  2:581939/125‑1 (MQ=255)                                        gCTCTTTTCATAGAGGAATTTGCCGTAATCCATTATACGACAAACCGGCGGCTCGGCGTTAGGGCTGATCTCGACTAAGCCTACTCCGGCTTCTTCTGCTTTCTCCAGAGCTTCTCTCAGACTca                                                                                          >  1:581939/1‑125 (MQ=255)                                          tctTTTCATAGAGGAATTTGCCGTAATCCATTATACGACAAACCGGCGGCTCGGCGTTAGGGCTGATCTCGACTAAGCCTACTCCGGCTTCTTCTGCTTTCTCCAGAGCTTCTCTCAGACTCACAATACCAAGCTGCTCg                                                                         <  1:567249/140‑1 (MQ=255)                                              ttCATAGAGGAATTTGCCGTAATCCATTATACGACAAACCGGCGGCTCGGCGTTAGGGCTGATCTCGACTAAGCCTACTCCGGCTTCTTCTGCTTTCTCCAGAGCTTCTCTCAGACTCACAATACCAAGCTGCTCGCCtt                                                                     >  1:542211/1‑140 (MQ=255)                                                 aTAGAGGAATTTGCCGTAATCCATTATACGACAAACCGGCGGCTCGGCGTTAGGGCTGATCTCGACTAAGCCTACTCCGGCTTCTTCTGCTTTCTCCAGAGCTTCTCTCAGACTCACAATACCAAGCTGCTCGCCTTCCa                                                                  <  2:626575/140‑1 (MQ=255)                                                      ggAATTTGCCGTAATCCATTATACGACAAACCGGCGGCTCGGCGTTAGGGCTGATCTCGACTAAGCCTACTCCGGCTTCTTCTGCTTTCTCCAGAGCTTCTCTCAGACTCACAATACCAAGCTGCTCGCCTTCCAGACCt                                                             >  2:166132/1‑140 (MQ=255)                                                                                cAAACCGGCGGCTCGGCGTTAGGGCTGATCTCGACTAAGCCTACTCCGGCTTCTTCTGCTTTCTCCAGAGCTTCTCTCAGACTCACAATACCAAGCTGCTCGCCTTCCAGACCTGTTAAGCGAACTTCCTGGGCGCGAAt                                   >  2:600570/1‑140 (MQ=255)                                                                                  aaCCGGCGGCTCGGCGTTAGGGCTGATCTCGACTAAGCCTACTCCGGCTTCTTCTGCTTTCTCCAGAGCTTCTCTCAGACTCACAATACCAAGCTGCTCGCCTTCCAGACCTGTTAAGCGAACTTCCTGGGCGCGAAttt                                 >  2:29904/1‑140 (MQ=255)                                                                                  aaCCGGCGGCTCGGCGTTAGGGCTGATCTCGACTAAGCCTACTCCGGCTTCTTCTGCTTTCTCCAGAGCTTCTCTCAGACTCACAATACCAAGCTGCTCGCCTCCCAGACCTGTTAAGCGAACTTCCTGGGCGCGAAttt                                 <  1:923243/140‑1 (MQ=255)                                                                                   aCCGGCGGCTCGGCGTTAGGGCTGATCTCGACTAAGCCTACTCCGGCTTCTTCTGCTTTCTCCAGAGCTTCTCTCAGACTCACAATACCAAGCTGCTCGCCTTCCAGACCTGTTAAGCGAACTTCCTGGGCGCGAATTTc                                <  1:600570/140‑1 (MQ=255)                                                                                       gcggcTCGGCGTTAGGGCTGATCTCGACTAAGCCTACTCCGGCTTCTTCTGCTTTCTCCAGAGCTTCTCTCAGACTCACAATACCAAGCTGCTCGCCTTCCAGACCTGTTAAGCGAACTTCCTGGGCGCGAATTTCGCCa                            <  2:366185/140‑1 (MQ=255)                                                                                       gcggcTCGGCGTTAGGGCTGATCTCGACTAAGCCTACTCCGGCTTCTTCTGCTTTCTCCAGAGCTTCTCTCAGACTCACAATACCAAGCTGCTCGCCTTCCAGACCTGTTAAGCGAACTTCCTGGGCGCGAATTTCGCCa                            <  2:542211/140‑1 (MQ=255)                                                                                           cTCGGCGTTAGGGCTGATCTCGACTAAGCCTACTCCGGCTTCTTCTGCTTTCTCCAGAGCTTCTCTCAGACTCACAATACCAAGCTGCTCGCCTTCCAGACCTGTTAAGCGAACTTCCTGGGCGCGAATTTCGCCATTGa                        <  1:166132/140‑1 (MQ=255)                                                                                                           aTCTCGACTAAGCCTACTCCGGCTTCTTCTGCTTTCTCCAGAGCTTCTCTCAGACTCACAATACCAAGCTGCTCGCCTTCCAGACCTGTTAAGCGAACTTCCTGGGCGCGAATTTCGCCATTGATACGGTTAGGgcgcgc        >  2:288667/1‑140 (MQ=255)                                                                                                                 aCTAAGCCTACTCCGGCTTCTTCTGCTTTCTCCAGAGCTTCTCTCAGACTCACAATACCAAGCTGCTCGCCTTCCAGACCTGTTAAGCGAACTTCCTGGGCGCGAATTTCGCCATTGATACGGTTAGGgcgc          <  2:280103/132‑1 (MQ=255)                                                                                                                 aCTAAGCCTACTCCGGCTTCTTCTGCTTTCTCCAGAGCTTCTCTCAGACTCACAATACCAAGCTGCTCGCCTTCCAGACCTGTTAAGCGAACTTCCTGGGCGCGAATTTCGCCATTGATACGGTTAGGgcgc          >  1:280103/1‑132 (MQ=255)                                                                                                                 aCTAAGCCTACTCCGGCTTCTTCTGCTTTCTCCAGAGCTTCTCTCAGACTCACAATACCAAGCTGCTCGCCTTCCAGACCTGTTAAGCGAACTTCCTGGGCGCGAATTTCGCCATTGATACGGTTAGGGCGCGCCGTTTg  >  2:41368/1‑140 (MQ=255)                                                                                                                       |                                                                                                                                      GATAACTTTTTGCTTTTTCTTCTGTTCCTTAGAAGACTTGCTCTTTTCATAGAGGAATTTGCCGTAATCCATTATACGACAAACCGGCGGCTCGGCGTTAGGGCTGATCTCGACTAAGTCTACTCCGGCTTCTTCTGCTTTCTCCAGAGCTTCTCTCAGACTCACAATACCAAGCTGCTCGCCTTCCAGACCTGTTAAGCGAACTTCCTGGGCGCGAATTTCGCCATTGATACGGTTAGGGCGCGCCGTTTG  >  NC_000913/1800366‑1800617

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑