Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,846,611	T→C	D117G (GAC→GGC)	topB ←	DNA topoisomerase III

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,846,611	0	T	C	100.0%	120.7 / NA	34	D117G (GAC→GGC)	topB	DNA topoisomerase III
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (15/19);  total (15/19)

CGGTACAAACTCACTGTTGGAACGAAGACGGTCGATCGCCCGCTCAACCGCCTGCGGGTTCAGGTCGTTTATCAAGCAACGCTGTACCTGCTGGCGCTTTTCCGGTGCCAGTTGCAGATAGTCCAGCACTTCATCCACCAGCAATTGCCCTTCACGATCCGGGTCCCCGGCGTGAACGATTTCGCTGGCTTCATGCAGGAACCGTTTGATGACGTTAAGTTGTTTGGTCACGGAGGGT  >  NC_000913/1846490‑1846727                                                                                                                          |                                                                                                                     cGGTACAAACTCACTGTTGGAACGAAGACGGTCGATCGCCCGCTCAACCGCCTGCGGGTTCAGGTCGTTTATCAAGCAACGCTGTACCTGCTGGCGCTTTTCCGGTGCCAGTTGCAGATAGCCCAGCACTTCATccacca                                                                                                    >  2:515324/1‑140 (MQ=255)            cACTGTTGGAACGAAGACGGTCAATCGCCCGCTCAACCGCCTGCGGGTTCAGGTCGTTTATCAAGCAACGCTGTACCTGCTGGCGCTTTTCCGGTGCCAGTTGCAGATAGCCCAGCACTTCATCCACCAGCAATTGCCCt                                                                                         <  1:165770/140‑1 (MQ=255)             aCTGTTGGAACGAAGACGGTCGATCGCCCGCTCAACCGCCTGCGGGTTCAGGTCGTTTATCAAGCAACGCTGTACCTGCTGGCGCTTTTCCGGTGCCAGTTGCAGATAGCCCAGCACTTCATCCACCAGCAATTGCCCtt                                                                                        >  1:282159/1‑140 (MQ=255)             aCTGTTGGAACGAAGACGGTCGATCGCCCGCTCAACCGCCTGCGGGTTCAGGTCGTTTATCAAGCAACGCTGTACCTGCTGGCGCTTTTCCGGTGCCAGTTGCAGATAGCCCAGCACTTCATCCACCAGCAATTGCCCtt                                                                                        >  2:361714/1‑140 (MQ=255)              cTGTTGGAACGAAGACGGTCGATCGCCCGCTCAACCGCCTGCGGGTTCAGGTCGTTTATCAAGCAACGCTGTACCTGCTGGCGCTTTTCCGGTGCCAGTTGCAGATAGCCCAGCACTTCATCCACCAGCAATTGCCCTTc                                                                                       >  2:69457/1‑140 (MQ=255)                   ggAACGAAGACGGTCGATCGCCCGCTCAACCGCCTGCGGGTTCAGGTCGTTTATCAAGCAACGCTGTACCTGCTGGCGCTTTTCCGGTGCCAGTTGCAGATAGCCCAGCACTTCATCCACCAGCAATTGc                                                                                            <  1:117626/130‑1 (MQ=255)                   ggAACGAAGACGGTCGATCGCCCGCTCAACCGCCTGCGGGTTCAGGTCGTTTATCAAGCAACGCTGTACCTGCTGGCGCTTTTCCGGTGCCAGTTGCAGATAGCCCAGCACTTCATCCACCAGCAATTGc                                                                                            >  2:117626/1‑130 (MQ=255)                    gAACGAAGACGGTCGATCGCCCGCTCAACCGCCTGCGGGTTCAGGTCGTTTATCAAGCAACGCTGTACCTGCTGGCGCTTTTCCGGTGCCAGTTGCAGATAGCCCAGCACTTCATCCACCAGCAATTGCCCTTCACGATc                                                                                 <  1:69457/140‑1 (MQ=255)                         aaGACGGTCGATCGCCCGCTCAACCGCCTGCGGGTTCAGGTCGTTTATCAAGCAACGCTGTACCTGCTGGCGCTTTTCCGGTGCCAGTTGCAGATAGCCCAGCACTTCATCCACCAGCAATTGCCCTTCACGATCCGGGt                                                                            >  2:177013/1‑140 (MQ=255)                                tCGATCGCCCGCTCAACCGCCTGCGGGTTCAGGTCGTTTATCAAGCAACGCTGTACCTGCTGGCGCTTTTCCGGTGCCAGTTGCAGATAGCCCAGCACTTCATCCACCAGCAATTGCCCTTCACGATCCGGGTCCCCGGc                                                                     <  1:89140/140‑1 (MQ=255)                                tCGATCGCCCGCTCAACCGCCTGCGGGTTCAGGTCGTTTATCAAGCAACGCTGTACCTGCTGGCGCTTTTCCGGTGCCAGTTGCAGATAGCCCAGCACTTCATCCACCAGCAATTGCCCTTCACGATCCGGGTCCCCGGc                                                                     <  1:177013/140‑1 (MQ=255)                                              aaCCGCCTGCGGGTTCAGGTCGTTTATCAAGCAACGCTGTACCTGCTGGCGCTTTTCCAGTGCCAGTTGCAGATAGCCCAGCACTTCATCCACCAGCAATTGCCCTTCACGATCCGGGTCCCCGGCGTGAACGATTTCGc                                                       <  1:195534/140‑1 (MQ=255)                                                ccGCCTGCGGGTTCAGGTCGTTTATCAAGCAACGCTGTACCTGCTGGCGCTTTTCCGGTGCCAGTTGCAGATAGCCCAGCACTTCATCCACCAGCAATTGCCCTTCACGATCCGGGTCCCCGGCGTGAACGATTTCGCTg                                                     <  2:504922/140‑1 (MQ=255)                                                ccGCCTGCGGGTTCAGGTCGTTTATCAAGCAACGCTGTACCTGCTGGCGCTTTTCCGGTGCCAGTTGCAGATAGCCCAGCACTTCATCCACCAGCAATTGCCCTTCACGATCCGGGTCCCCAGCGTGAACGATTTCGCTg                                                     >  1:473458/1‑140 (MQ=255)                                                    cTGCGGGTTCAGGTCGTTTATCAAGCAACGCTGTACCTGCTGGCGCTTTTCCGGTGCCAGTTGCAGATAGCCCAGCACTTCATCCACCAGCAATTGCCCTTCACGATCCGGGTCCCCGGCGTGAACGATTTCGCTGGCtt                                                 <  2:282159/140‑1 (MQ=255)                                                    cTGCGGGTTCAGGTCGTTTATCAAGCAACGCTGTACCTGCTGGCGCTTTTCCGGTGCCAGTTGCAGATAGCCCAGCACTTCATCCACCAGCAATTGCCCTTCACGATCCGGGTCCCCGGCGTGAACGATTTCGCTGGCtt                                                 <  1:961760/140‑1 (MQ=255)                                                    cTGCGGGTTCAGGTCGTTTATCAAGCAACGCTGTACCTGCTGGCGCTTTTCCGGTGCCAGTTGCAGATAGCCCAGCACTTCATCCACCAGCAATTGCCCTTCACGATCCGGGTCCCCGGCGCGAACGATTTCGCTGGCtt                                                 <  1:471542/140‑1 (MQ=255)                                                     tGCGGGTTCAGGTCGTTTATCAAGCAACGCTGTACCTGCTGGCGCTTTTCCGGTGCCAGTTGCAGATAGCCCAGCACTTCATCCACCAGCAATTGCCCTTCACGATCCGGGTCCCCGGCGTGAACGATTTCGCTGGCTTc                                                >  1:482191/1‑140 (MQ=255)                                                      gCGGGTTCAGGTCGTTTATCAAGCAACGCTGTACCTGCTGGCGCTTTTCCGGTGCCAGTTGCAGATAGCCCAGCACTTCATCCACCAGCAATTGCCCTTCACGATCCGGGTCCCCGGCGTGAACGATTTCGCTGGCTTCa                                               <  1:605471/140‑1 (MQ=255)                                                        gggTTCAGGTCGTTTATCAAGCAACGCTGTACCTGCTGGCGCTTTTCCGGTGCCAGTTGCAGATAGCCCAGCACTTCATCCACCAGCAATTGCCCTTCACGATCCGGGTCCCCGGCGTGAACGATTTCGCTGGCTTCATg                                             >  1:91041/1‑140 (MQ=255)                                                        gggTTCAGGTCGTTTATCAAGCAACGCTGTACCTGCTGGCGCTTTTCCGGTGCCAGTTGCAGATAGCCCAGCACTTCATCCACCAGCAATTGCCCTTCACGATCCGGGTCCCCGGCGTGAACGATTTCGCTGGCTTCATg                                             <  1:515324/140‑1 (MQ=255)                                                            tCAGGTCGTTTATCAAGCAACGCTGTACCTGCTGGCGCTTTTCCGGTGCCAGTTGCAGATAGCCCAGCACTTCATCCACCAGCAATTGCCCTTCACGATCCGGGTCCCCGGCGTGAACGATTTCGCTGGCTTCATGCAgg                                         <  2:482191/140‑1 (MQ=255)                                                              aGGTCGTTTATCAAGCAACGCTGTACCTGCTGGCGCTTTTCCGGTGCCAGTTGCAGATAGCCCAGCACTTCATCCACCAGCAATTGCCCTTCACGATCCGGGTCCCCGGCGTGAACGATTTCGCTGGCTTCATGCAGGaa                                       <  2:733070/140‑1 (MQ=255)                                                                      tATCAAGCAACGCTGTACCTGCTGGCGCTTTTCCGGTGCCAGTTGCAGATAGCCCAGCACTTCATCCACCAGCAATTGCCCTTCACGATCCGGGTCCCCGGCGTGAACGATTTCGCTGGCTTCATGCAGGAACCGTTtga                               <  1:508431/140‑1 (MQ=255)                                                                      tATCAAGCAACGCTGTACCTGCTGGCGCTTTTCCGGTGCCAGTTGCAGATAGCCCAGCACTTCATCCACCAGCAATTGCCCTTCACGATCCGGGTCCCCGGCGTGAACGATTTCGCTGGCTTCATGCAGGAACCGTTtga                               <  1:162451/140‑1 (MQ=255)                                                                                cGCTGTACCTGCTGGCGCTTTTCCGGTGCCAGTTGCAGATAGCCCAGCACTTCATCCACCAGCAATTGCCCTTCACGATCCGGGTCCCCGGCGTGAACGATTTCGCTGGCTTCATGCAGGAACCGTTTGATGACGTTAAg                     >  2:124221/1‑140 (MQ=255)                                                                                  cTGTACCTGCTGGCGCTTTTCCGGTGCCAGCTGCAGTTAGCCCAGCACTTCATCCACCAGCAATTGCCCTTCACGATCCGGGTCCCCGGCGTGAACGATTTCGCTGGCTTCATGCAGGAACCGTTTGATGACGTTacggt                   >  2:296082/1‑136 (MQ=255)                                                                                      aCCTGCTGGCGCTTTTCCGGTGCCAGTTGCAGATAGCCCAGCACTTCATCCACCAGCAATTGCCCTTCACGATCCGGGTCCCCGGCGTGAACGATTTCGCTGGCTTCATGCAGGAACCGTTTGATGACGTTAAGTTGttt               >  1:272323/1‑140 (MQ=255)                                                                                            tgGCGCTTTTCCGGTGCCAGTTGCAGATAGCCCAGCACTTCATCCACCAGCAATTGCCCTTCACGATCCGGGTCCCCGGCGTGAACGATTTCGCTGGCTTCATGCAGGAACCGTTTGATGACGTTAAGTTGTTTGGTCAc         >  1:505865/1‑140 (MQ=255)                                                                                             ggCGCTTTTCCGGTGCCAGTTGCAGATAGCCCAGCACTTCATCCACCAGCAATTGCCCTTCACGATCCGGGTCCCCGGCGTGAACGATTTCGCTGGCTTCATGCAGGAACCGTTTGATGACGTTAAGTTGTTTGGTCACg        >  2:263330/1‑140 (MQ=255)                                                                                              gcgcTTTTCCGGTGCCAGTTGCAGATAGCCCAGCACTTCATCCACCAGCAATTGCCCTTCACGATCCGGGTCCCCGGCGTGAACGATTTCGCTGGCTTCATGCAGGAACCGTTTGATGACGTTAAGTTGTTTGGTCACgg       <  2:954690/140‑1 (MQ=255)                                                                                               cgcTTTTCCGGTGCCAGTTGCAGATAGCCCAGCACTTCATCCACCAGCAATTGCCCTTCACGATCCGGGTCCCCGGCGTGAACGATTTCGCTGGCTTCATGCAGGAACCGTTTGATGACGTTAAGTTGTTTGGTCACGGa      >  1:882789/1‑140 (MQ=255)                                                                                               cgcTTTTCCGGTGCCAGTTGCAGATAGCCCAGCACTTCATCCACCAGCAATTGCCCTTCACGATCCGGGTCCCCGGCGTGAACGATTTCGCTGGCTTCATGCAGGAACCGTTTGATGACGTTAAGTTGTTTGGTCACGGa      >  1:460834/1‑140 (MQ=255)                                                                                                   tttCCGGTGCCAGTTGCAGATAGCCCAGCACTTCATCCACCAGCAATTGCCCTTCACGATCCGGGTCCCCGGCGTGAACGATTTCGCTGGCTTCATGCAGGAACCGTTTGATGACGTTAAGTTGTTTGGTCACGGAGGGt  <  2:91041/140‑1 (MQ=255)                                                                                                   tttCCGGTGCCAGTTGCAGATAGCCCAGCACTTCATCCACCAGCAATTGCCCTTCACGATCCGGGTCCCCGGCGTGAACGATTTCGCTGGCTTCATGCAGGAACCGTTTGATGACGTTAAGTTGTTTGGTCACGGAGGGt  <  1:194909/140‑1 (MQ=255)                                                                                                                          |                                                                                                                     CGGTACAAACTCACTGTTGGAACGAAGACGGTCGATCGCCCGCTCAACCGCCTGCGGGTTCAGGTCGTTTATCAAGCAACGCTGTACCTGCTGGCGCTTTTCCGGTGCCAGTTGCAGATAGTCCAGCACTTCATCCACCAGCAATTGCCCTTCACGATCCGGGTCCCCGGCGTGAACGATTTCGCTGGCTTCATGCAGGAACCGTTTGATGACGTTAAGTTGTTTGGTCACGGAGGGT  >  NC_000913/1846490‑1846727

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑