Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,860,480	A→G	F285L (TTC→CTC)	ydjL ←	putative zinc‑binding dehydrogenase YdjL

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,860,480	0	A	G	100.0%	112.7 / NA	32	F285L (TTC→CTC)	ydjL	putative zinc‑binding dehydrogenase YdjL
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (13/19);  total (13/19)

TGCGTGATCATCGGTTTGACTTTGATAGCGCCGCTGGCTAATAGCCTGATAGCGTTACGCCATGAGGTGGAGTCATAGGCCATATGCCCAATGATGCTTTTGTTCCAGGCGGTAATGTCATTAATCGAGAAATCAAGAGGTTTGAAGCCCATTCCAACGCGTACCACTTCCCCGTTCGGGCGGAGCATTTCGATGGCTTGTTTCAGTGCGATATTGGCACCGGAGCATTCAATCACCAGTCCCAGATTGTCTTTGCCACAAA  >  NC_000913/1860350‑1860611                                                                                                                                   |                                                                                                                                    tGCGTGATCATCGGTTTGACTTTGATAGCGACGCTGGCTAATAGCCTGATAGCGTTACGCCATGAGGTGGAGTCATAGGCCATATGCCCAATGATGCTTTTGTTCCAGGCGGTAATGTCATTAATCGAGAGATCAAGAgg                                                                                                                            >  2:551217/1‑140 (MQ=255)     tGATCATCGGTTTGACTTTGATAGCGCCGCTGGCTAATAGCCTGATAGCGTTACGCCATGAGGTGGAGTCATAGGCCATATGCCCAATGATGCTTTTGTTCCAGGCGGTAATGTCATTAATCGAGAGATCAAGAGGTTTg                                                                                                                        >  1:438820/1‑140 (MQ=255)              gTTTGACTTTGATAGCGCCGCTGGCTAATAGCCTGATAGCGTTACGCCATGAGGTGGAGTCATAGGCCATATGCCCAATGATGCTTTTGTTCCAGGCGGTAATGTCATTAATCGAGAGATCAAGAGGTTTGAAGCCCAtt                                                                                                               <  2:250261/140‑1 (MQ=255)                 tGACTTTGATAGCGCCGCTGGCTAATAGCCTGATAGCGTTACGCCATGAGGTGGAGTCATAGGCCATATGCCCAATGATGCTTTTGTTCCAGGCGGTAATGTCATTAATCGAGAGATCAAGAGGTTTGAAGCCCATTCCa                                                                                                            >  2:795698/1‑140 (MQ=255)                   aCTTTGATAGCGCCGCTGGCTAATAGCCTGATAGCGTTACGCCATGAGGTGGAGTCATAGGCCATATGCCCAATGATGCTTTTGTTCCAGGCGGTAATGTCATTAATCGAGAGATCAAGAGGTTTGAAGCCCATTCCAAc                                                                                                          >  2:70248/1‑140 (MQ=255)                      ttGATAGCGCCGCTGGCTAATAGCCTGATAGCGTTACGCCATGAGGTGGAGTCATAGGCCATATGCCCAATGATGCTTTTGTTCCAGGCGGTAATGTCATTAATCGAGAGATCAAGAGGTTTGAAGCCCATTCCAAcgcg                                                                                                       <  2:226650/140‑1 (MQ=255)                        gATAGCGCCGCTGGCTAATAGCCTGATAGCGTTACGCCATGAGGTGGAGTCATAGGCCATATGCCCAATGATGCTTTTGTTCCAGGCGGTAATGTCATTAATCGAGAGATCAAGAGGTTTGAAGCCCATTCCAACGCGTa                                                                                                     <  1:612852/140‑1 (MQ=255)                           aGCGCCGCTGGCTAATAGCCTGATAGCGTTACGCCATGAGGTGGAGTCATAGGCCATATGCCCAATGATGCTTTTGTTCCAGGCGGTAATGTCATTAATCGAGAGATCAAGAGGTTTGAAGCCCATTCCAACGCGTACCa                                                                                                  <  1:442385/140‑1 (MQ=255)                             cgccgcTGGCTAATAGCCTGATAGCGTTACGCCATGAGGTGGAGTCATAGGCCATATGCCCAATGATGCTTTTGTTCCAGGCGGTAATGTCATTAATCGAGAGATCAAGAGGTTTGAAGCCCATTCCAACGCGTACCACt                                                                                                >  1:343525/1‑140 (MQ=255)                                               tGATAGCGTTACGCCATGAGGTGGAGTCATAGGCCATATGCCCAATGATGCTTTTGTTCCAGGCGGTAATGTCATTAATCGAGAGATCAAGAGGTTTGAAGCCCATTCCAACGCGTACCACTTCCCCGTTCGGGCGGAGc                                                                              <  2:438820/140‑1 (MQ=255)                                                      gTTACGCCATGAGGTGGAGTCATAGGCCATATGCCCAATGATGCTTTTGTTCCAGGCGGTAATGTCATTAATCGAGAGATCAAGAGGTTTGAAGCCCATTCCAACGCGTACCACTTCCCCGTTCGGGCGGAGCATTTCGa                                                                       <  2:937266/140‑1 (MQ=255)                                                      gTTACGCCATGAGGTGGAGTCATAGGCCATATGCCCAATGATGCTTTTGTTCCAGGCGGTAATGTCATTAATCGAGAGATCAAGAGGTTTGAAGCCCATTCCAACGCGTACCACTTCCCCGTTCGGGCGGAGCATTTCGa                                                                       <  2:722927/140‑1 (MQ=255)                                                        tACGCCATGAGGTGGAGTCATAGGCCATATGCCCAATGATGCTTTTGTTCCAGGCGGTAATGTCATTAATCGAGAGATCAAGAGGTTTGAAGCCCATTCCAACGCGTACCACTTCCCCGTTCGGGCGGAGCATTTCGATg                                                                     <  1:551217/140‑1 (MQ=255)                                                          cGCCATGAGGTGGAGTCATAGGCCATATGCCCAATGATGCTTTTGTTCCAGGCGGTAATGTCATTAATCGAGAGATCAAGAGGTTTGAAGCCCATTCCAACGCGTACCACTTCCCCGTTCGGGCGGAGCATTTCGATGGc                                                                   >  1:412091/1‑140 (MQ=255)                                                          cGCCATGAGGTGGAGTCATAGGCCATATGCCCAATGATGCTTTTGTTCCAGGCGGTAATGTCACTAATCGAGAGATCAAGAGGTTTGAAGCCCATTCCAACGCGTACCACTTCCCCGTTCGGGCGGAGCATTTCGATGGc                                                                   >  1:229663/1‑140 (MQ=255)                                                                      gAGTCATAGGCCATATGCCCAATGATGCTTTTGTTCCAGGCGGTAATGTCATTAATCGAGAGATCAAGAGGTTTGAAGCCCATTCCAACGCGTACCACTTCCCCGTTCGGGCGGAGCATTTCGATGGCTTGTTTCAGTGc                                                       <  2:247556/140‑1 (MQ=255)                                                                      gAGTCATAGGCCATATGCCCAATGATGCTTTTGTTCCAGGCGGTAATGTCATTAATCGAGAGATCAAGAGGTTTGAAGCCCATTCCAACGCGTACCACTTCCCCGTTCGGGCGGAGCATTTCGATGGCTTGTTTCAGTGc                                                       <  1:75899/140‑1 (MQ=255)                                                                              ggCCATATGCCCAATGATGCTTTTGTTCCAGGCGGTAATGTCATTAATCGAGAGATCAAGAGGTTTGAAGCCCATTCCAACGCGTACCACTTCCCCGTTCGGGCGGAGCATTTCGATGGCTTGTTTCAGTGCGATATTgg                                               <  2:412091/140‑1 (MQ=255)                                                                              ggCCATATGCCCAATGATGCTTTTGTTCCAGGCGGTAATGTCATTAATCGAGAGATCAAGAGGTTTGAAGCCCATTCCAACGCGTACCACTTCCCCGTTCGGGCGGAGCATTTCGATGGCTTGTTTCAGTGCGATATTgg                                               >  1:53003/1‑140 (MQ=255)                                                                              ggCCATATGCCCAATGATGCTTTTGTTCCAGGCGGTAATGTCACTAATCGAGAGATCAAGAGGTTTGAAGCCCATTCCAACGCGTACCACTTCCCCGTTCGGGCGGAGCATTTCGATGGCTTGTTTCAGTGCGATATTgg                                               <  2:229663/140‑1 (MQ=255)                                                                                   tatGCCCAATGATGCTTTTGTTCCAGGCGGTAATGTCATTAATCGAGAGATCAAGAGGTTTGAAGCCCATTCCAACGCGTACCACTTCCCCGTTCGGGCGGAGCATTTCGATGGCTTGTTTCAGTGCGATATTGGCACCg                                          <  2:912440/140‑1 (MQ=255)                                                                                   tatGCCCAATGATGCTTTTGTTCCAGGCGGTAATGTCATTAATCGAGAGATCAAGAGGTTTGAAGCCCATTCCAACGCGTACCACTTCCCCGTTCGGGCGGAGCATTTCGATGGCTTGTTTCAGTGCGATATTGGCACCg                                          >  1:262707/1‑140 (MQ=255)                                                                                       cccAATGATGCTTTTGTTCCAGGCGGTAATGTCATTAATCGAGAGATCAAGAGGTTTGAAGCCCATTCCAACGCGTACCACTTCCCCGTTCGGGCGGAGCATTTCGATGGCTTGTTTCAGTGCGATATTGGCACCGGAGc                                      <  2:342571/140‑1 (MQ=255)                                                                                       cccAATGATGCTTTTGTTCCAGGCGGTAATGTCATTAATCGAGAGATCAAGAGGTTTGAAGCCCATTCCAACGCGTACCACTTCCCCGTTCGGGCGGAGCATTTCGATGGCTTGTTTCAGTGCGATATTGGCACCGGAGc                                      >  1:508820/1‑140 (MQ=255)                                                                                                 cTTTTGTTCCAGGCGGTAATGTCATTAATCGAGAGATCAAGAGGTTTGAAGCCCATTCCAACGCGTACCACTTCCCCGTTCGGGCGGAGCATTTCGATGGCTTGTTTCAGTGCGATATTGGCACCGGAGCATTCAATCAc                            <  2:53003/140‑1 (MQ=255)                                                                                                   tttGTTCCAGGCGGTAATGTCATTAATCGAGAGATCAAGAGGTTTGAAGCCCATTCCAACGCGTACCACTTCCCCGTTCGGGCGGAGCATTTCGATGGCTTGTTTCAGTGCGATATTGGCACCGGAGCATTCAATCACCa                          <  2:508820/140‑1 (MQ=255)                                                                                                           aGGCGGTAATGTCATTAATCGAGAGATCAAGAGGTTTGAAGCCCATTCCAACGCGTACCACTTCCCCGTTCGGGCGGAGCATTTCGATGGCTTGTTTCAGTGCGATATTGGCACCGGAGCATTCAATCACCAGTCCCAGa                  <  1:70248/140‑1 (MQ=255)                                                                                                                 tAATGTCATTAATCGAGAGATCAAGAGGTTTGAAGCCCATTCCAACGCGTACCACTTCCCCGTTCGGGCGGAGCATTTCGATGGCTTGTTTCAGTGCGATATTGGCACCGGAGCATTCAATCAc                            >  2:700170/1‑124 (MQ=255)                                                                                                                 tAATGTCATTAATCGAGAGATCAAGAGGTTTGAAGCCCATTCCAACGCGTACCACTTCCCCGTTCGGGCGGAGCATTTCGATGGCTTGTTTCAGTGCGATATTGGCACCGGAGCATTCAATCAc                            <  1:700170/124‑1 (MQ=255)                                                                                                                 tAATGTCATTAATCGAGAGATCAAGAGGTTTGAAGCCCATTCCAACGCGTACCACTTCCCCGTTCGGGCGGAGCATTTCGATGGCTTGTTTCAGTGCGATATTGGCACCGGAGCATTCAATCACCAGTCCCAGATTGTCt            >  2:241867/1‑140 (MQ=255)                                                                                                                 tAATGTCATTAATCGAGAGATCAAGAGGTTTGAAGCCCATTCCAACGCGTACCACTTCCCCGTTCGGGCGGAGCATTTCGATGGCTTGTTTCAGTGCGATATTGGCACCGGAGCATTCAATCACCAGTCCCAGATTGTCt            >  2:737792/1‑140 (MQ=255)                                                                                                                           aaTCGAGAGATCAAGAGGTTTGAAGCCCATTCCAACGCGTACCACTTCCCCGTTCGGGCGGAGCATTTCGATGGCTTGTTTCAGTGCGATATTGGCACCGGAGCATTCAATCACCAGTCCCAGATTGTCTTTGCCACaaa  <  2:343525/140‑1 (MQ=255)                                                                                                                                   |                                                                                                                                    TGCGTGATCATCGGTTTGACTTTGATAGCGCCGCTGGCTAATAGCCTGATAGCGTTACGCCATGAGGTGGAGTCATAGGCCATATGCCCAATGATGCTTTTGTTCCAGGCGGTAATGTCATTAATCGAGAAATCAAGAGGTTTGAAGCCCATTCCAACGCGTACCACTTCCCCGTTCGGGCGGAGCATTTCGATGGCTTGTTTCAGTGCGATATTGGCACCGGAGCATTCAATCACCAGTCCCAGATTGTCTTTGCCACAAA  >  NC_000913/1860350‑1860611

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑