| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 1,873,384 | A→G | K448K (AAA→AAG) | dgcJ → | putative diguanylate cyclase DgcJ |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,873,384 | 0 | A | G | 97.7% | 149.7 / ‑5.0 | 44 | K448K (AAA→AAG) | dgcJ | putative diguanylate cyclase DgcJ |
| Reads supporting (aligned to +/- strand): ref base A (0/1); new base G (18/25); total (18/26) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01 | |||||||||||
TTACGTTATTAGCTCAGGCGATTAAACAGTCGATTCGTAAAAGTGATTATGCCATCCGACTCGGTGGCGATG‑AATTCTGCATCATTCTTGTCGATTCGACGCCGCAAATTGCAGCACAACTGCCTGAACGTATCGAAAAACGTCTGCAACATATCGCGCCGCAGAAAGAGATCGGCTTCTCTTCCGGTATTTACGCGATGAAAGAAAACGATACGTTACATGATGCGTATAAAGCTTCCGATGAGCGTTTATATGTCAATAAGCAG > NC_000913/1873245‑1873510 | ttACGTTATTAGCTCAGGCGATTAAACAGTCGATTCGTAAAAGGGATTATGCATCCCGCCCCGGT‑GCGAAGAAATCCTCAATAATTTTTGCCGTTTCG‑CCCCCCAAATTGCCGCACAACCGGCTGAAAGGAACGgaaaag > 2:720397/1‑139 (MQ=255) cGTTATTAGCTCAGGCGATTAAACAGTCGATTCGTAAAAGTGATTATGCCATCCGACTCGGTGGCGATG‑AATTCTGCATCATTCTTGTCGATTCGACGCCGCAAATTGCAGCACAACTGCCTGAACGTATCGAAAAGCGt < 2:797545/140‑1 (MQ=255) tattaGCTCAGGCGATTACACCGTCGATTCGTAAAAGTGATTATGCCATCCGACTCGGTGGCGATG‑AATTCTGACTAATTCTTGTCGATTCGACGCCGCAAATTGCAGAACAACTGCCTGAACGTATCGAAAAGCGTCTg < 2:749300/140‑1 (MQ=255) tCAGGCGATTAAACAGTCGATTCGTAAAAGTGATTATGCCATCCGACTCGGTGGCGATG‑AATTCTGCATCATTCTTGTCGATTCGACGCCGCAAATTGCAGCACAACTGCCTGAACGTATCGAAAAGCGTCTGCAACata > 2:206164/1‑140 (MQ=255) aGGCGATTAAACAGTCGATTCGTAAAAGTGATTATGCCATCCGACTCGGTGGCGATG‑AATTCTGCATCATTCTTGTCGATTCGACGCCGCAAATTGCAGCACAACTGCCTGAACGTATCGAAAAGCGTCTGCAACATATc > 2:619156/1‑140 (MQ=255) ggCGATTAAACAGTCGATTCGTAAAAGTGATTATGCCATCCGACTCGGTGGCGATG‑AATTCTGCATCATTCTTGTCGATTCGACGCCGCAAATTGCAGCACAACTGCCTGAACGTATCGAAAAGCGTCTGCAACATATcg > 2:402296/1‑140 (MQ=255) gCGATTAAACAGTCGATTCGTAAAAGTGATTATGCCATCCGACTCGGTGGCGATG‑AATTCTGCATCATTCTTGTCGATTCGACGCCGCAAATTGCAGCACAACTGCCTGAACGTATCGAAAAGCGTCTGCAACATATcgc > 1:946513/1‑140 (MQ=255) gCGATTAAACAGTCGATTCGTAAAAGTGATTATGCCATCCGACTCGGTGGCGATG‑AATTCTGCATCATTCTTGTCGATTCGACGCCGCAAATTGCAGCACAACTGCCTGAACGTATCGAAAAGCGTCTGCAACATATcgc > 1:880496/1‑140 (MQ=255) ttAAACAGTCGATTCGTAAAAGTGATTATGCCATCCGACTCGGTGGCGATG‑AATTCTGCATCATTCTTGTCGATTCGACGCCGCAAATTGCAGCACAACTGCCTGAACGTATCGAAAAGCGTCTGCAACATATCGcgccg < 1:220874/140‑1 (MQ=255) tAAACAGTCGATTCGTAAAAGTGATTATGCCATCCGACTCGGTGGCGATG‑AATTCTGCATCATTCTTGTCGATTCGACGCCGCAAATTGCAGCACAACTGCCTGAACGTATCGAAAAGCGTCTGCAACATATCGcgccgc > 2:346178/1‑140 (MQ=255) gATTCGTAAAAGTGATTATGCCATCCGACTCGGTGGCGATG‑AATTCTGCATCATTCTTGTCGATTCGACGCCGCAAATTGCAGCACAACTGCCTGAACGTATCGAAAAGCGTCTGCAACATATCGCGCCGCAGAAAgaga < 2:704759/140‑1 (MQ=255) gATTCGTAAAAGTGATTATGCCATCCGACTCGGTGGCGATG‑AATTCTGCATCATTCTTGTCGATTCGACGCCGCAAATTGCAGCACAACTGCCTGAACGTATCGAAAAGCGTCTGCAACATATCGCGCCGCAGAAAgaga > 2:658176/1‑140 (MQ=255) aaaaGTGATTATGCCATCCGACTCGGTGGCGATG‑AATTCTGCATCATTCTTGTCGATTCGACGCCGCAAATTGCAGCACAACTGCCTGAACGTATCGAAAAGCGTCTGCAACATATCGCGCCGCAGAAAGAGATCGGCtt < 2:946513/140‑1 (MQ=255) aaaaGTGATTATGCCATCCGACTCGGTGGCGATG‑AATTCTGCATCATTCTTGTCGATTCGACGCCGCAAATTGCAGCACAACTGCCTGAACGTATCGAAAAGCGTCTGCAACATATCGCGCCGCAGAAAGAGATCGGCtt < 1:399247/140‑1 (MQ=255) aaaaGTGATTATGCCATCCGACTCGGTGGCGATG‑AATTCTGCATCATTCTTGTCGATTCGACGCCGCAAATTGCAGCACAACTGCCTGAACGTATCGAAAAGCGTCTGCAACATATCGCGCCGCAGAAAGAGATCGGCtt < 2:880496/140‑1 (MQ=255) gATTATGCCATCCGACTCGGTGGCGATG‑AATTCTGCATCATTCTTGTCGATTCGACGCCGCAAATTGCAGCACAACTGCCTGAACGTATCGAAAAGCGTCTGCAACATATCGCGCCGCAGAAAGAGATCGGCTTCTCTTc < 1:619156/140‑1 (MQ=255) aTTATGCCATCCGACTCGGTGGCGATG‑AATTCTGCATCATTCTTGTCGATTCGACGCCGCAAATTGCAGCACAACTGCCTGAACGTATCGAAAAGCGTCTGCAACATATCGCGCCGCAGAAAGAGATGGGCTTCTCTTcc > 2:745607/1‑140 (MQ=255) aTTATGCCATCCGACTCGGTGGCGATG‑AATTCTGCATCATTCTTGTCGATTCGACGCCGCAAATTGCAGCACAACTGCCTGAACGTATCGAAAAGCGTCTGCAACATATCGCGCCGCAGAAAGAGATCGGCTTCTCTTcc > 2:861933/1‑140 (MQ=255) ttATGCCATCCGACTCGGTGGCGATG‑AATTCTGCATCATTCTTGTCGATTCGACGCCGCAAATTGCAGCACAACTGCCTGAACGTATCGAAAAGCGTCTGCAACATATCGCGCCGCAGAAAGAGATCGGCTTCTCTTCCg > 2:670253/1‑140 (MQ=255) ccGACTCGGTGGCGATG‑AATTCTGCATCATTCTTGTCGATTCGACGCCGCAAATTGCAGCACAACTGCCTGAACGTATCGAAAAACGTCTGCAACATATCGCGCCGCAGAAAGAGATCGGCTGCTCCTCCGGTATTTAcg < 2:707508/140‑1 (MQ=255) gACTCGGTGGCGATG‑AATTCTGCATCATTCTTGTCGATTCGACGCCGCAAATTGCAGCACAACTGCCTGAACGTATCGAAAAGCGTCTGCAACATATCGCGCCGCAGAAAGAGATCGGCTTCTCTTCCGGTATTTAcgcg < 1:611467/140‑1 (MQ=255) aCTCGGTGGCGATG‑AATTCTGCATCATTCTTGTCGATTCGACGCCGCAAATTGCAGCACAACTGCCTGAACGTATCGAAAAGCGTCTGCAACATATCGCGCCGCAGAAAGAGATCGGCTTCTCTTCCGGTATTTACGCGa > 1:471825/1‑140 (MQ=255) aCTCGGTGGCGATG‑AATTCTGCATCATTCTTGTCGATTCGACGCCGCAAATTGCAGCACAACTGCCTGAACGTATCGAAAAGCGTCTGCAACATATCGCGCCGCAGAAAGAGATCGGCTTCTCTTCCGGTATTTACGCGa < 1:346178/140‑1 (MQ=255) ggTGGCGATG‑AATTCTGCATCATTCTTGTCGATTCGACGCCGCAAATTGCAGCACAACTGCCTGAACGTATCGAAAAGCGTCTGCAACATATCGCGCCGCAGAAAGAGATCGGCTTCTCTTCCGGTATTTACGCGATgaa > 2:542861/1‑140 (MQ=255) tGGCGATG‑AATTCTGCATCATTCTTGTCGATTCGACGCCGCAAATTGCAGCACAACTGCCTGAACGTATCGAAAAGCGTCTGCAACATATCGCGCCGCAGAAAGAGATCGGCTTCTCTTCCGGTATTTACGCGATgaaag > 1:534078/1‑140 (MQ=255) gATG‑AATTCTGCATCATTCTTGTCGATTCGACGCCGCAAATTGCAGCACAACTGCCTGAACGTATCGAAAAGCGTCTGCAACATATCGCGCCGCAGAAAGAGATCGGCTTCTCTTCCGGTATTTACGCGATGAAAGaaaa < 2:471825/140‑1 (MQ=255) tG‑AATTCTGCATCATTCTTGTCGATTCGACGCCGCAAATTGCAGCACAACTGCCTGAACGTATCGAAAAGCGTCTGCAACATATCGCGCCGCAGAAAGAGATCGGCTTCTCTTCCGGTATTTACGCGATGAAAGAAAACg < 2:534078/140‑1 (MQ=255) aaTTCTGCATCATTCTTGTCGATTCGACGCCGCAAATTGCAGCACAACTGCCTGAACGTATCGAAAAGCGTCTGCAACATATCGCGCCGCAGAAAGAGATCGGCTTCTCTTCCGGTATTTACGCGATGAAAGAAAACGAt < 2:796813/140‑1 (MQ=255) aaTTCTGCATCATTCTTGTCGATTCGACGCCGCAAATTGCAGCACAACTGCCTGAACGTATCGAAAAGCGTCTGCAACATATCGCGCCGCAGAAAGAGATCGGCTTCTCTTCCGGTATTTACGCGATGAAAGAAAACGAt < 1:467612/140‑1 (MQ=255) aaTTCTGCATCATTCTTGTCGATTCGACGCCGCAAATTGCAGCACAACTGCCTGAACGTATCGAAAAGCGTCTGCAACATATCGCGCCGCAGAAAGAGATCGGCTTCTCTTCCGGTATTTACGCGATGAAAGAAAACGAt < 1:670253/140‑1 (MQ=255) aaTTCTGCATCATTCTTGTCGATTCGACGCCGCAAATTGCAGCACAACTGCCTGAACGTATCGAAAAGCGTCTGCAACATATCGCGCCGCAGAAAGAGATCGGCTTCTCTTCCGGTATTTACGCGATGAAAGAAAACGAt < 1:542861/140‑1 (MQ=255) catcatTCTTGTCGATTCGACGCCGCAAATTGCAGCACAACTGCCTGAACGTATCGAAAAGCGTCTGCAACATATCGCGCCGCAGAAAGAGATCGGCTTCTCTTCCGGTATTTACGCGATGAAAGAAAACGATACGTTAc < 2:409913/140‑1 (MQ=255) catTCTTGTCGATTCGACGCCGCAAATTGCAGCACAACTGCCTGAACGTATCGAAAAGCGTCTGCAACATATCGCGCCGCAGAAAGAGATCGGCTTCTCTTCCGGTATTTACGCGATGAAAGAAAACGATACGTTACatg > 1:408872/1‑140 (MQ=255) atTCTTGTCGATTCGACGCCGCAAATTGCAGCACAACTGCCTGAACGTATCGAAAAGCGTCTGCAACATATCGCGCCGCAGAAAGAGATCGGCTTCTCTTCCGGTATTTACGCGATGAAAGAAAACGATACGTTACatga < 1:848900/140‑1 (MQ=255) ttCTTGTCGATTCGACGCCGCAAATTGCAGCACAACTGCCTGAACGTATCGAAAAGCGTCTGCAACATATCGCGCCGCAGAAAGAGATCGGCTTCTCTTCCGGTATTTACGCGATGAAAGAAAACGATAc < 1:587996/130‑1 (MQ=255) ttCTTGTCGATTCGACGCCGCAAATTGCAGCACAACTGCCTGAACGTATCGAAAAGCGTCTGCAACATATCGCGCCGCAGAAAGAGATCGGCTTCTCTTCCGGTATTTACGCGATGAAAGAAAACGATAc > 2:587996/1‑130 (MQ=255) ttGTCGATTCGACGCCGCAAATTGCAGCACAACTGCCTGAACGTATCGAAAAGCGTCTGCAACATATCGCGCCGCAGAAAGAGATCGGCTTCTCTTCCGGTATTTACGCGATGAAAGAAAACGATACGTTACATGATGCg > 1:710374/1‑140 (MQ=255) gTCGATTCGACGCCGCAAATTGCAGCACAACTGCCTGAACGTATCGAAAAGCGTCTGCAACATATCGCGCCGCAGAAAGAGATCGGCTTCTCTTCCGGTATTTACGCGATGAAAGAAAACGATACGTTACATGATGCGta > 2:474271/1‑140 (MQ=255) cgcAAATTGCAGCACAACTGCCTGAACGTATCGAAAAGCGTCTGCAACATATCGCGCCGCAGAAAGAGATCGGCTTCTCTTCCGGTATTTACGCGATGAAAGAAAACGATACGTTACATGATGCGTATAAAGCTTCCGAt < 1:861933/140‑1 (MQ=255) gcAAATTGCAGCACAACTGCCTGAACGTATCGAAAAGCGTCTGCAACATATCGCGCCGCAGAAAGAGATCGGCTTCTCTTCCGGTATTTACGCGATGAAAGAAAACGATACGTTACATGATGCGTATAAAGCTTCCGATg < 2:710374/140‑1 (MQ=255) cAAATTGCAGCACAACTGCCTGAACGTATCGAAAAGCGTCTGCAACATATCGCGCCGCAGAAAGAGATCGGCTTCTCTTCCGGTATTTACGCGATGAAAGAAAACGATACGTTACATGATGCGTATAAAGCTTCCGATGa < 2:408872/140‑1 (MQ=255) aaTTGCAGCACAACTGCCTGAACGTATCGAAAAGCGTCTGCAACATATCGCGCCGCAGAAAGAGATCGGCTTCTCTTCCGGTATTTACGCGATGAAAGAAAACGATACGTTACATGATGCGTATAAAGCTTCCGATGAGc < 1:402296/140‑1 (MQ=255) cTGAACGTATCGAAAAGCGTCTGCAACATATCGCGCCGCAGAAAGAGATGGGCTTCTCTTCCGGTATTTACGCGATGAAAGAAAACGATACGTTACATGATGCGTATAAAGCTTCCGATGAGCGTTTATATGTCAATAAg < 1:745607/140‑1 (MQ=255) tGAACGTATCGAAAAGCGTCTGCAACATATCGCGCCGCAGAAAGAGATCGGCTTCTCTTCCGGTATTTACGCGATGAAAGAAAACGATACGTTACATGATGCGTATAAAGCTTCCGATGAGCGTTTATATGTCAATAAGc > 1:570993/1‑140 (MQ=255) aaCGTATCGAAAAGCGTCTGCAACATATCGCGCCGCAGAAAGAGATCGGCTTCTCTTCCGGTATTTACGCGATGAAAGAAAACGATACGTTACATGATGCGTATAAAGCTTCCGATGAGCGTTTATATGTCAATAAGCAg < 1:206164/140‑1 (MQ=255) | TTACGTTATTAGCTCAGGCGATTAAACAGTCGATTCGTAAAAGTGATTATGCCATCCGACTCGGTGGCGATG‑AATTCTGCATCATTCTTGTCGATTCGACGCCGCAAATTGCAGCACAACTGCCTGAACGTATCGAAAAACGTCTGCAACATATCGCGCCGCAGAAAGAGATCGGCTTCTCTTCCGGTATTTACGCGATGAAAGAAAACGATACGTTACATGATGCGTATAAAGCTTCCGATGAGCGTTTATATGTCAATAAGCAG > NC_000913/1873245‑1873510 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |