Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	350,112	C→T	T34I (ACC→ATC)	prpC →	2‑methylcitrate synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	350,112	0	C	T	100.0%	255.6 / NA	72	T34I (ACC→ATC)	prpC	2‑methylcitrate synthase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (37/35);  total (37/35)

TCTCGACCCTACAAATGATAACAATGACGAGGACAACATGAGCGACACAACGATCCTGCAAAACAGTACCCATGTCATTAAACCGAAAAAATCTGTGGCACTTTCTGGCGTTCCGGCGGGCAATACGGCGCTCTGCACCGTGGGTAAAAGTGGCAATGACCTGCATTACCGCGGCTACGATATTCTTGATCTGGCGAAACATTGCGAATTTGAAGAAGTGGCGCATCTGCTGATCCACGGCAAACTGCCGACCCGTGACGAACTCGCCGCTTA  >  NC_000913/349975‑350247                                                                                                                                          |                                                                                                                                        tctcGACCCTACAAATGATAACAATGACGAGGACAACATGAGCGACACAACGATCCTGCAAAACAGTACCCATGTCATTAAACCGAAAAAATCTGTGGCACTTTCTGGCGTTCCGGCGGGCAATACGGCGCTCTGCATCg                                                                                                                                       >  1:228273/1‑140 (MQ=255)    cGACCCTACAAATGATAACAATGACGAGGACAACATGAGCGACACAACGATCCTGCAAAACAGTACCCATGTCATTAAACCGAAAAAATCTGTGGCACTTTCTGGCGTTCCGGCGGGCAATACGGCGCTCTGCATCGTgg                                                                                                                                    >  1:287065/1‑140 (MQ=255)           aCAAATGATAACAATGACGAGGACAACATGAGCGACACAACGATCCTGCAAAACAGTACCCATGTCATTAAACCGAAAAAATCTGTGGCACTTTCTGGCGTTCCGGCGGGCAATACGGCGCTCTGCATCGTGGGTAAAAg                                                                                                                             >  1:764160/1‑140 (MQ=255)            cAAATGATAACAATGACGAGGACAACATGAGCGACACAACGATCCTGCAAAACAGTACCCATGTCATTAAACCGAAAAAATCTGTGGCACTTTCTGGCGTTCCGGCGGGCAATACGGCGCTCTGCATCGTGGGTAAAAGt                                                                                                                            >  2:563729/1‑140 (MQ=255)                tGATAACAATGACGAGGACAACATGAGCGACACAACGATCCTGCAAAACAGTACCCATGTCATTAAACCGAAAAAATCTGTGGCACTTTCTGGCGTTCCGGCGGGCAATACGGCGCTCTGCATCGTGGGTAAAAGTGGCa                                                                                                                        <  2:228273/140‑1 (MQ=255)                tGATAACAATGACGAGGACAACATGAGCGACACAACGATCCTGCAAAACAGTACCCATGTCATTAAACCGAAAAAATCTGTGGCACTTTCTGGCGTTCCGGCGGGCAATACGGCGCTCTGCATCGTGGGTAAAAGTGGCa                                                                                                                        <  1:792633/140‑1 (MQ=255)                 gATAACAATGACGAGGACAACATGAGCGACACAACGATCCTGCAAAACAGTACCCATGTCATTAAACCGAAAAAATCTGTGGCACTTTCTGGCGTTCCGGCGGGCAATACGGCGCTCTGCATCGTGGGTAAAAGTGGCaa                                                                                                                       >  2:880066/1‑140 (MQ=255)                   tAACAATGACGAGGACAACATGAGCGACACAACGATCCTGCAAAACAGTACCCATGTCATTAAACCGAAAAAATCTGTGGCACTTTCTGGCGTTCCGGCGGGCAATACGGCGCTCTGCATCGTGGGTAAAAGTGGCAATg                                                                                                                     <  1:563729/140‑1 (MQ=255)                    aaCAATGACGAGGACAACATGAGCGACACAACGATCCTGCAAAACAGTACCCATGTCATTAAACCGAAAAAATCTGTGGCACTTTCTGGTGTTCCGGCGGGCAATACGGCGCTCTGCATCGTGGGTAAAAGTGGCAATGa                                                                                                                    >  2:963588/1‑140 (MQ=255)                    aaCAATGACGAGGACAACATGAGCGACACAACGATCCTGCAAAACAGTACCCATGTCATTAAACCGAAAAAATCTGTGGCACTTTCTGGCGTTCCGGCGGGCAATACGGCGCTCTGCATCGTGGGTAAAAGTGGCAATGa                                                                                                                    >  1:706528/1‑140 (MQ=255)                    aaCAATGACGAGGACAACATGAGCGACACAACGATCCTGCAAAACAGTACCCATGTCATTAAACCGAAAAAATCTGTGGCACTTTCTGGCGTTCCGGCGGGCAATACGGCGCTCTGCATCGTGGGTAAAAGTGGCAATGa                                                                                                                    >  1:248594/1‑140 (MQ=255)                     aCAATGACGAGGACAACATGAGCGACACAACGATCCTGCAAAACAGTACCCATGTCATTAAACCGAAAAAATCTGTGGCACTTTCTGGCGTTCCGGCGGGCAATACGGCGCTCTGCATCGTGGGTAAAAGTGGCAATGAc                                                                                                                   <  1:430001/140‑1 (MQ=255)                      cAATGACGAGGACAACATGAGCGACACAACGATCCTGCAAAACAGTACCCATGTCATTAAACCGAAAAAATCTGTGGCACTTTCTGGCGTTCCGGCGGGCAATACGGCGCTCTGCATCGTGGGTAAAAGTGGCAATGAcc                                                                                                                  >  1:396002/1‑140 (MQ=255)                           aCGAGGACAACATGAGCGACACAACGATCCTGCAAAACAGTACCCATGTCATTAAACCGAAAAAATCTGTGGCACTTTCTGGCGTTCCGGCGGGCAATACGGCGCTCTGCATCGTGGGTAAAAGTGGCAATGACCTGCAt                                                                                                             >  2:191818/1‑140 (MQ=255)                            cGAGGACAACATGAGCGACACAACGATCCTGCAAAACAGTACCCATGTCATTAAACCGAAAAAATCTGTGGCACTTTCTGGCGTTCCGGCGGGCAATACGGCGCTCTGCATCGTGGGTAAAAGTGGCAATGAc                                                                                                                   >  2:404495/1‑133 (MQ=255)                            cGAGGACAACATGAGCGACACAACGATCCTGCAAAACAGTACCCATGTCATTAAACCGAAAAAATCTGTGGCACTTTCTGGCGTTCCGGCGGGCAATACGGCGCTCTGCATCGTGGGTAAAAGTGGCAATGAc                                                                                                                   <  1:404495/133‑1 (MQ=255)                             gAGGACAACATGAGCGACACAACGATCCTGCAAAACAGTACCCATGTCATTAAACCGAAAAAATCTGTGGCACTTTCTGGCGTTCCGGCGGGCAATACGGCGCTCTGCATCGTGGGTAAAAGTGGCAATGACCTGCATTa                                                                                                           >  1:23934/1‑140 (MQ=255)                              aGGACAACATGAGCGACACAACGATCCTGCAAAACAGTACCCATGTCATTAAACCGAAAAAATCTGTGGCACTTTCTGGCGTTCCGGCGGGCAATACGGCGCTCTGCATCGTGGGTAAAAGTGGCa                                                                                                                        >  1:266837/1‑126 (MQ=255)                              aGGACAACATGAGCGACACAACGATCCTGCAAAACAGTACCCATGTCATTAAACCGAAAAAATCTGTGGCACTTTCTGGCGTTCCGGCGGGCAATACGGCGCTCTGCATCGTGGGTAAAAGTGGCa                                                                                                                        <  2:266837/126‑1 (MQ=255)                                gACAACATGAGCGACACAACGATCCTGCAAAACAGTACCCATGTCATTAAACCGAAAAAATCTGTGGCACTTTCTGGCGTTCCGGCGGGCAATACGGCGCTCTGCATCGTGGGTAAAAGTGGCAATGACCTGCATTACcg                                                                                                        <  1:33485/140‑1 (MQ=255)                                  caacaTGAGCGACACAACGATCCTGCAAAACAGTACCCATGTCATTAAACCGAAAAAATCTGTGGCACTTTCTGGCGTTCCGGCGGGCAATACGGCGCTCTGCATCGTGGGTAAAAGTGGCAATGACCTGCATTACcgcg                                                                                                      >  2:172215/1‑140 (MQ=255)                                     caTGAGCGACACAACGATCCTGCAAAACAGTACCCATGTCATTAAACCGAAAAAATCTGTGGCACTTTCTGGCGTTCCGGCGGGCAATACGGCGCTCTGCATCGTGGGTAAAAGTGGCAATGACCTGCATTACCGCGGCt                                                                                                   >  2:74159/1‑140 (MQ=255)                                     caTGAGCGACACAACGATCCTGCAAAACAGTACCCATGTCATTAAACCGAAAAAATCTGTGGCACTTTCTGGCGTTCCGGCGGGCAATACGGCGCTCTGCATCGTGGGTAAAAGTGGCAATGACCTGCATTACCGCGGCt                                                                                                   >  1:332285/1‑140 (MQ=255)                                      aTGAGCGACACAACGATCCTGCAAAACAGTACCCATGTCATTAAACCGAAAAAATCTGTGGCACTTTCTGGCGTTCCGGCGGGCAATACGGCGCTCTGCATCGTGGGTAAAAGTGGCAATGACCTGCATTACCGCGGCTa                                                                                                  <  1:880066/140‑1 (MQ=255)                                      aTGAGCGACACAACGATCCTGCAAAACAGTACCCATGTCATTAAACCGAAAAAATCTGTGGCACTTTCTGGCGTTCCGGCGGGCAATACGGCGCTCTGCATCGTGGGTAAAAGTGGCAATGACCTGCATTACCGCGGCTa                                                                                                  <  2:287065/140‑1 (MQ=255)                                        gAGCGACACAACGATCCTGCAAAACAGTACCCATGTCATTAAACCGAAAAAATCTGTGGCACTTTCTGGCGTTCCGGCGGGCAATACGGCGCTCTGCATCGTGGGTAAAAGTGGCAATGACCTGCATTACCGCGGCTACg                                                                                                <  1:74159/140‑1 (MQ=255)                                         aGCGACACAACGATCCTGCAAAACAGTACCCATGTCATTAAACCGAAAAAATCTGTGGCACTTTCTGGCGTTCCGGCGGGCAATACGGCGCTCTGCATCGTGGGTAAAAGTGGCAATGACCTGCATTACCGCGGCTACGa                                                                                               <  2:248594/140‑1 (MQ=255)                                          gCGACACAACGATCCTGCAAAACAGTACCCATGTCATTAAACCGAAAAAATCTGTGGCACTTTCTGGCGTTCCGGCGGGCAATACGGCGCTCTGCATCGTGGGTAAAAGTGGCAATGACCTGCATTACCGCGGCTa                                                                                                  <  2:142634/136‑1 (MQ=255)                                          gCGACACAACGATCCTGCAAAACAGTACCCATGTCATTAAACCGAAAAAATCTGTGGCACTTTCTGGCGTTCCGGCGGGCAATACGGCGCTCTGCATCGTGGGTAAAAGTGGCAATGACCTGCATTACCGCGGCTa                                                                                                  >  1:142634/1‑136 (MQ=255)                                             acacAACGATCCTGCAAAACAGTACCCATGTCATTAAACCGAAAAAATCTGTGGCACTTTCTGGCGTTCCGGCGGGCAATACGGCGCTCTGCATCGTGGGTAAAAGTGGCAATGACCTGCATTACCGCGGCTACGa                                                                                               <  1:889896/136‑1 (MQ=255)                                             acacAACGATCCTGCAAAACAGTACCCATGTCATTAAACCGAAAAAATCTGTGGCACTTTCTGGCGTTCCGGCGGGCAATACGGCGCTCTGCATCGTGGGTAAAAGTGGCAATGACCTGCATTACCGCGGCTACGa                                                                                               >  2:889896/1‑136 (MQ=255)                                             acacAACGATCCTGCAAAACAGTACCCATGTCATTAAACCGAAAAAATCTGTGGCACTTTCTGGCGTTCCGGCGGGCAATACGGCGCTCTGCATCGTGGGTAAAAGTGGCAATGACCTGCATTACCGCGGCTACGATAtt                                                                                           >  1:455288/1‑140 (MQ=255)                                             acacAACGATCCTGCAAAACAGTACCCATGTCATTAAACCGAAAAAATCTGTGGCACTTTCTGGCGTTCCGGCGGGCAATACGGCGCTCTGCATCGTGGGTAAAAGTGGCAATGACCTGCATTACCGCGGCTACGATAtt                                                                                           >  1:333437/1‑140 (MQ=255)                                                caACGATCCTGCAAAACAGTACCCATGTCATTAAACCGAAAAAATCTGTGGCACTTTCTGGCGTTCCGGCGGGCAATACGGCGCTCTGCATCGTGGGTAAAAGTGGCAATGACCTGCATTACCGCGGCTACGATATTCtt                                                                                        >  1:38492/1‑140 (MQ=255)                                                  aCGATCCTGCAAAACAGTACCCATGTCATTAAACCGAAAAAATCTGTGGCACTTTCTGGCGTTCCGGCGGGCAATACGGCGCTCTGCATCGTGGGTAAAAGTGGCAATGACCTGCATTACCGCGGCTACGATATTCTTGa                                                                                      <  2:526598/140‑1 (MQ=255)                                                    gATCCTGCAAAACAGTACCCATGTCATTAAACCGAAAAAATCTGTGGCACTTTCTGGCGTTCCGGCGGGCAATACGGCGCTCTGCATCGTGGGTAAAAGTGGCAATGACCTGCATTACCGCGGCTACGATATTCTTGATc                                                                                    <  1:310885/140‑1 (MQ=255)                                                        cTGCAAAACAGTACCCATGTCATTAAACCGAAAAAATCTGTGGCACTTTCTGGCGTTCCGGCGGGCAATACGGCGCTCTGCATCGTGGGTAAAAGTGGCAATGACCTGCATTACCGCGGCTACGATATTCTTGATCTGGc                                                                                >  1:564167/1‑140 (MQ=255)                                                        cTGCAAAACAGTACCCATGTCATTAAACCGAAAAAATCTGTGGCACTTTCTGGCGTTCCGGCGGGCAATACGGCGCTCTGCATCGTGGGTAAAAGTGGCAATGACCTGCATTACCGCGGCTACGATATTCTTGATCTGGc                                                                                >  2:249077/1‑140 (MQ=255)                                                        cTGCAAAACAGTACCCATGTCATTAAACCGAAAAAATCTGTGGCACTTTCTGGCGTTCCGGCGGGCAATACGGCGCTCTGCATCGTGGGTAAAAGTGGCAATGACCTGCATTACCGCGGCTACAATATTCTTGATCTGGc                                                                                >  1:56880/1‑140 (MQ=255)                                                                 aGTACCCATGTCATTAAACCGAAAAAATCTGTGGCACTTTCTGGCGTTCCGGCGGGCAATACGGCGCTCTGCATCGTGGGTAAAAGTGGCAATGACCTGCATTACCGCGGCTACGATATTCTTGATCTGGCGAAACATTg                                                                       <  2:720281/140‑1 (MQ=255)                                                                  gTACCCATGTCATTAAACCGAAAAAATCTGTGGCACTTTCTGGCGTTCCGGCGGGCAATACGGCGCTCTGCATCGTGGGTAAAAGTGGCAATGACCTGCATTACCGCGGCTACGATATTCTTGATCTGGCGAAACATTGc                                                                      >  2:263680/1‑140 (MQ=255)                                                                   tACCCATGTCATTAAACCGAAAAAATCTGTGGCACTTTCTGGCGTTCCGGCGGGCAATACGGCGCTCTGCATCGTGGGTAAAAGTGGCAATGACCTGCATTACCGCGGCTACGATATTCTTGATCTGGCGAAACATTGCg                                                                     >  2:551791/1‑140 (MQ=255)                                                                    aCCCATGTCATTAAACCGAAAAAATCTGTGGCACTTTCTGGCGTTCCGGCGGGCAATACGGCGCTCTGCATCGTGGGTAAAAGTGGCAATGACCTGCATTACCGCGGCTACAATATTCTTGATCTGGCGAAACATTGCGa                                                                    <  2:56880/140‑1 (MQ=255)                                                                     cccATGTCATTAAACCGAAAAAATCTGTGGCACTTTCTGGCGTTCCGGCGGGCAATACGGCGCTCTGCATCGTGGGTAAAAGTGGCAATGACCTGCATTACCGCGGCTACGATATTCTTGATCTGGCGAAACATTGCGaa                                                                   >  1:146703/1‑140 (MQ=255)                                                                        aTGTCATTAAACCGAAAAAATCTGTGGCACTTTCTGGCGTTCCGGCGGGCAATACGGCGCTCTGCATCGTGGGTAAAAGTGGCAATGACCTGCATTACCGCGGCTACGATATTCTTGATCTGGCGAAACATTGCGAAttt                                                                >  2:25409/1‑140 (MQ=255)                                                                           tCATTAAACCGAAAAAATCTGTGGCACTTTCTGGCGTTCCGGCGGGCAATACGGCGCTCTGCATCGTGGGTAAAAGTGGCAATGACCTGCATTACCGCGGCTACGATATTCTTGATCTGGCGAAACATTGCGAATTTgaa                                                             <  2:899822/140‑1 (MQ=255)                                                                           tCATTAAACCGAAAAAATCTGTGGCACTTTCTGGCGTTCCGGCGGGCAATACGGCGCTCTGCATCGTGGGTAAAAGTGGCAATGACCTGCATTACCGCGGCTACGATATTCTTGATCTGGCGAAACATTGCGAATTTgaa                                                             >  1:899822/1‑140 (MQ=255)                                                                             aTTAAACCGAAAAAATCTGTGGCACTTTCTGGCGTTCCGGCGGGCAATACGGCGCTCTGCATCGTGGGTAAAAGTGGCAATGACCTGCATTACCGCGGCTACGATATTCTTGATCTGGCGAAACATTGCGAATTTgaaga                                                           <  2:564167/140‑1 (MQ=255)                                                                               tAAACCGAAAAAATCTGTGGCACTTTCTGGCGTTCCGGCGGGCAATACGGCGCTCTGCATCGTGGGTAAAAGTGGCAATGACCTGCATTACCGCGGCTACGATATTCTTGATCTGGCGAAACATTGCGAATTTGaagaag                                                         >  2:940044/1‑140 (MQ=255)                                                                                    cGAAAAAATCTGTGGCACTTTCTGGCGTTCCGGCGGGCAATACGGCGCTCTGCATCGTGGGTAAAAGTGGCAATGACCTGCATTACCGCGGCTACGATATTCTTGATCTGGCGAAACATTGCGAATTTGAAGAAGTGgcg                                                    >  1:526189/1‑140 (MQ=255)                                                                                     gAAAAAATCTGTGGCACTTTCTGGCGTTCCGGCGGGCAATACGGCGCTCTGCATCGTGGGTAAAAGTGGCAATGACCTGCATTACCGCGGCTACGATATTCTTGATCTGGCGAAACATTGCGAATTTGAAGAAGTGgcgc                                                   <  2:332285/140‑1 (MQ=255)                                                                                     gAAAAAATCTGTGGCACTTTCTGGCGTTCCGGCGGGCAATACGGCGCTCTGCATCGTGGGTAAAAGTGGCAATGACCTGCATTACCGCGGCTACGATATTCTTGATCTGGCGAAACATTGCGAATTTGAAGAAGTGgcgc                                                   <  1:249077/140‑1 (MQ=255)                                                                                     gAAAAAATCTGTGGCACTTTCTGGCGTTCCGGCGGGCAATACGGCGCTCTGCATCGTGGGTAAAAGTGGCAATGACCTGCATTACCGCGGCTACGATATTCTTGATCTGGCGAAACATTGCGAATTTGAAGAAGTGgcgc                                                   <  2:56596/140‑1 (MQ=255)                                                                                     gAAAAAATCTGTGGCACTTTCTGGCGTTCCGGCGGGCAATACGGCGCTCTGCATCGTGGGTAAAAGTGGCAATGACCTGCATTACCGCGGCTACGATATTCTTGATCTGGCGAAACATTGCGAATTTGAAGAAGTGgcgc                                                   <  2:526189/140‑1 (MQ=255)                                                                                     gAAAAAATCTGTGGCACTTTCTGGCGTTCCGGCGGGCAATACGGCGCTCTGCATCGTGGGTAAAAGTGGCAATGACCTGCATTACCGCGGCTACGATATTCTTGATCTGGCGAAACATTGCGAATTTGAAGAAGTGgcgc                                                   <  2:455288/140‑1 (MQ=255)                                                                                     gAAAAAATCTGTGGCACTTTCTGGCGTTCCGGCGGGCAATACGGCGCTCTGCATCGTGGGTAAAAGTGGCAATGACCTGCATTACCGCGGCTACGATATTCTTGATCTGGCGAAACATTGCGAATTTGAAGAAGTGgcgc                                                   <  2:333437/140‑1 (MQ=255)                                                                                      aaaaaaTCTGTGGCACTTTCTGGCGTTCCGGCGGGCAATACGGCGCTCTGCATCGTGGGTAAAAGTGGCAATGACCTGCATTACCGCGGCTACGATATTCTTGATCTGGCGAAACATTGCGAATTTGAAGAAGTGGCGCa                                                  <  1:172215/140‑1 (MQ=255)                                                                                      aaaaaaTCTGTGGCACTTTCTGGCGTTCCGGCGGGCAATACGGCGCTCTGCATCGTGGGTAAAAGTGGCAATGACCTGCATTACCGCGGCTACGATATTCTTGATCTGGCGAAACATTGCGAATTTGAAGAAGTGGCGCa                                                  >  1:938057/1‑140 (MQ=255)                                                                                                  gCACTTTCTGGCGTTCCGGCGGGCAATACGGCGCTCTGCATCGTGGGTAAAAGTGGCAATGACCTGCATTACCGCGGCTACGATATTCTTGATCTGGCGAAACATTGCGAATTTGAAGAAGTGGCGCATCTGCTGATCCa                                      <  2:706528/140‑1 (MQ=255)                                                                                                   cACTTTCTGGCGTTCCGGCGGGCAATACGGCGCTCTGCATCGTGGGTAAAAGTGGCAATGACCTGCATTACCGCGGCTACGATATTCTTGATCTGGCGAAACATTGCGAATTTGAAGAAGTGGCGCATCTGCTGATCCAc                                     >  1:329682/1‑140 (MQ=255)                                                                                                       ttCTGGTGTTCCGGCGGGCAATACGGCGCTCTGCATCGTGGGTAAAAGTGGCAATGACCTGCATTACCGCGGCTACGATATTCTTGATCTGGCGAAACATTGCGAATTTGAAGAAGTCGCGCATCTGCTGATCCACGGCa                                 <  1:963588/140‑1 (MQ=255)                                                                                                       ttCTGGCGTTCCGGCGGGCAATACGGCGCTCTGCATCGTGGGTAAAAGTGGCAATGACCTGCATTACCGCGGCTACGATATTCTTGATCTGGCGAAACATTGCGAATTTGAAGAAGTGGCGCATCTGCTGATCCACGGCa                                 <  2:38492/140‑1 (MQ=255)                                                                                                       ttCTGGCGTTCCGGCGGGCAATACGGCGCTCTGCATCGTGGGTAAAAGTGGCAATGACCTGCATTACCGCGGCTACGATATTCTTGATCTGGCGAAACATTGCGAATTTGAAGAAGTGGCGCATCTGCTGATCCACGGCa                                 <  1:191818/140‑1 (MQ=255)                                                                                                            gCGTTCCGGCGGGCAATACGGCGCTCTGCATCGTGGGTAAAAGTGGCAATGACCTGCATTACCGCGGCTACGATATTCTTGATCTGGCGAAACATTGCGAATTTGAAGAAGTGGCGCATCTGCTGATCCACGGCAAACTg                            <  1:263680/140‑1 (MQ=255)                                                                                                              gTTCCGGCGGGCAATACGGCGCTCTGCATCGTGGGTAAAAGTGGCAATGACCTGCATTACCGCGGCTACGATATTCTTGATCTGGCGAAACATTGCGAATTTGAAGAAGTGGCGCATCTGCTGATCCACGGCAAACTGcc                          <  2:329682/140‑1 (MQ=255)                                                                                                                tCCGGCGGGCAATACGGCGCTCTGCATCGTGGGTAAAAGTGGCAATGACCTGCATTACCGCGGCTACGATATTCTTGATCTGGCGAAACATTGCGAATTTGAAGAAGTGGCGCATCTGCTGATCCACGGCAAACTGCCGa                        >  1:888686/1‑140 (MQ=255)                                                                                                                 ccGGCGGGCAATACGGCGCTCTGCATCGTGGGTAAAAGTGGCAATGACCTGCATTACCGCGGCTACGATATTCTTGATCTGGCGAAACATTGCGAATTTGAAGAAGTGGCGCATCTGCTGATCCACGGCAAACTGCCGAc                       >  1:473172/1‑140 (MQ=255)                                                                                                                                 cgcTCTGCATCGTGGGTAAAAGTGGCAATGACCTGCATTACCGCGGCTACGATATTCTTGATCTGGCGAAACATTGCGAATTTGAAGAAGTGGCGCATCTGCTGATCCACGGCAAACTGCCGACCCGTGACGAACTcgcc       <  1:25409/140‑1 (MQ=255)                                                                                                                                  gcTCTGCATCGTGGGTAAAAGTGGCAATGACCTGCATTACCGCGGCTACGATATTCTTGATCTGGCGAAACATTGCGAATTTGAAGAAGTGGCGCATCTGCTGATCCACGGCAAACTGCCGACCCGTGACGAACTcgccg      <  2:146703/140‑1 (MQ=255)                                                                                                                                    tctGCATCGTGGGTAAAAGTGGCAATGACCTGCATTACCGCGGCTACGATATTCTTGATCTGGCGAAACATTGCGAATTTGAAGAAGTGGCGCATCTGCTGATCCACGGCAAACTGCCGACCCGTGACGAACTcg         <  2:636393/135‑1 (MQ=255)                                                                                                                                    tctGCATCGTGGGTAAAAGTGGCAATGACCTGCATTACCGCGGCTACGATATTCTTGATCTGGCGAAACATTGCGAATTTGAAGAAGTGGCGCATCTGCTGATCCACGGCAAACTGCCGACCCGTGACGAACTcg         >  1:636393/1‑135 (MQ=255)                                                                                                                                      tGCATCGTGGGTAAAAGTGGCAATGACCTGCATTACCGCGGCTACGATATTCTTGATCTGGCGAAACATTGCGAATTTGAAGAAGTGGCGCATCTGCTGATCCACGGCAAACTGCCGACCCGTGACGAACTCGCCGCTTa  >  2:263604/1‑140 (MQ=255)                                                                                                                                          |                                                                                                                                        TCTCGACCCTACAAATGATAACAATGACGAGGACAACATGAGCGACACAACGATCCTGCAAAACAGTACCCATGTCATTAAACCGAAAAAATCTGTGGCACTTTCTGGCGTTCCGGCGGGCAATACGGCGCTCTGCACCGTGGGTAAAAGTGGCAATGACCTGCATTACCGCGGCTACGATATTCTTGATCTGGCGAAACATTGCGAATTTGAAGAAGTGGCGCATCTGCTGATCCACGGCAAACTGCCGACCCGTGACGAACTCGCCGCTTA  >  NC_000913/349975‑350247

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑