Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,892,742	T→C	N165S (AAC→AGC)	yoaA ←	putative 5' to 3' DNA helicase implicated in DNA repair

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,892,742	0	T	C	100.0%	171.9 / NA	48	N165S (AAC→AGC)	yoaA	putative 5' to 3' DNA helicase implicated in DNA repair
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (24/24);  total (24/24)

ACTCTCTTTAACCACCATATCCGCCAGAAAGAGATGATGGTTTACCACCACCACATCGGCGTCCATCGCTTTTTTACGTGCTTTGACCACAAAGCAATCTTTATACATCGGGCAGTCGCTGCCAAGACAGTTGTCGTTGGTGCTGGTGACCAGCGGCCACGCCTGTGAATCTTCCGCCACGCTGACGCAGGTGCTGATATCACCATCGACTGTTTGATTAGACCAGGAGCGCAGCAGGATCACATCGCTTAAGATTTGTACCGGCAGATCG  >  NC_000913/1892606‑1892876                                                                                                                                         |                                                                                                                                       aCTCTCTTTAACCACCATATCCGCCAGAAAGAGATGATGGTTTACCACCACCACATCGGCGTCCATCGCTTTTTTACGTGCTTTGACCACAAAGCAATCTTTATACATCGGGCAGTCGCTGCCAAGACAGTTGTCGCTgg                                                                                                                                     <  2:455194/140‑1 (MQ=255)      ctTTAACCACCATATCCGCCAGAAAGAGATGATGGTTTACCACCACCACATCGGCGTCCATCGCTTTTTTACGTGCTTTGACCACAAAGCAATCTTTATACATCGGGCAGTCGCTGCCAAGACAGTTGTCGCTGGTGCTg                                                                                                                                <  2:851894/140‑1 (MQ=255)               ccaTATCCGCCAGAAAGAGATGATGGTTTACCACCACCACATCGGCGTCCATCGCTTTTTTACGTGCTTTGACCACAAAGCAATCTTTATACATCGGGCAGTCGCTGCCAAGACAGTTGTCGCTGGTGCTTGTGACCAGc                                                                                                                       >  1:323975/1‑140 (MQ=255)               ccaTATCCGCCAGAAAGAGATGATGGTTTACCACCACCACATCGGCGTCCATCGCTTTTTTACGTGCTTTGACCACAAAGCAATCTTTATACATCGGGCAGTCGCTGCCAAGACAGTTGTCGCTGGTGCTGGTGACCAGc                                                                                                                       >  2:98378/1‑140 (MQ=255)               ccaTATCCGCCAGAAAGAGATGATGGTTTACCACCACCACATCGGCGTCCATCGCTTTTTTACGTGCTTTGACCACAAAGCAATCTTTATACATCGGGCAGTCGCTGCCAAGACAGTTGTCGCTGGTGCTGGTGACCAGc                                                                                                                       >  2:880333/1‑140 (MQ=255)               ccaTATCCGCCAGAAAGAGATGATGGTTTACCACCACCACATCGGCGTCCATCGCTTTTTTACGTGCTTTGACCACAAAGCAATCTTTATACATCGGGCAGTCGCTGCCAAGACAGTTGTCGCTGGTGCTGGTGACCAGc                                                                                                                       >  2:759757/1‑140 (MQ=255)                 atatCCGCCAGAAAGAGATGATGGTTTACCACCACCACATCGGCGTCCATCGCTTTTTTACGTGCTTTGACCACAAAGCAATCTTTATACATCGGGCAGTCGCTGCCAAGACAGTTGTCGCTGGTGCTGGTGACCAGCgg                                                                                                                     >  2:856836/1‑140 (MQ=255)                 acatCCGCCAGAAAGAGATGATGGTTTACCACCACCACATCGGCGTCCATCGCTTTTTTACGTGCTTTGACCACAAAGCAATCTTTATACATCGGGCAGTCGCTGCCAAGACAGTTGTCGCTGGTGCTGGTGACCAGCgg                                                                                                                     >  1:662112/3‑140 (MQ=255)                  tatCCGCCAGAAAGAGATGATGGTTTACCACCACCACATCGGCGTCCATCGCTTTTTTACGTGCTTTGACCACAAAGCAATCTTTATACATCGGGCAGTCGCTGCCAAGACAGTTGTCGCTGGTGCTGGTGACCAGCGGc                                                                                                                    <  1:635820/140‑1 (MQ=255)                     ccGCCAGAAAGAGATGATGGTTTACCACCACCACATCGGCGTCCATCGCTTTTTTACGTGCTTTGACCACAAAGCAATCTTTATACATCGGGCAGTCGCTGCCAAGACAGTTGCCGCTGGTGCTGGTGACCAGCGGCCAc                                                                                                                 >  2:716426/1‑140 (MQ=255)                      cGCCAGAAAGAGATGATGGTTTACCACCACCACATCGGCGTCCATCGCTTTTTTACGTGCTTTGACCACAAAGCAATCTTTATACATCGGGCAGTCGCTGCCAAGACAGTTGTCGCTGGTGCTGGTGACCAGCGGCCACg                                                                                                                <  1:98378/140‑1 (MQ=255)                      cGCCAGAAAGAGATGATGGTTTACCACCACCACATCGGCGTCCATCGCTTTTTTACGTGCTTTGACCACAAAGCAATCTTTATACATCGGGCAGTCGCTGCCAAGACAGTTGTCGCTGGTGCTGGTGACCAGCGGCCACg                                                                                                                <  1:251232/140‑1 (MQ=255)                            aaaGAGATGATGGTTTACCACCACCACATCGGCGTCCATCGCTTTTTTACGTGCTTTGACCACAAAGCAATCTTTATACATCGGGCAGTCGCTGCCAAGACAGTTGTCGCTGGTGCTGGTGACCAGCGGCCACCCCtgtg                                                                                                          >  2:287732/1‑140 (MQ=255)                             aaGAGATGATGGTTTACCACCACCACATCGGCGTCCATCGCTTTTTTACGTGCTTTGACCACAAAGCAATCTTTATACATCGGGCAGTCGCTGCCAAGACAGTTGTCGCTGGTGCTGGTGACCAGCGGCCACGCCTGTGa                                                                                                         <  2:953451/140‑1 (MQ=255)                                   tgatgGTTTACCACCACCACATCGGCGTCCATCGCTTTTTTACGTGCTTTGACCACAAAGCAATCTTTATACATCGGGCAGTCGCTGCCAAGACAGTTGTCGCTGGTGCTGGTGACCAGCGGCCACGCCTGTGAATCTTc                                                                                                   >  1:428728/1‑140 (MQ=255)                                      tgGTTTACCACCACCACATCGGCGTCCATCGCTTTTTTACGTGCTTTGACCACAAAGCAATCTTTATACATCGGGCAGTCGCTGCCAAGACAGTTGTCGCTGGTGCTGGTGACCAGCGGCCACGCCTGTGAATCTTCCGc                                                                                                <  1:868353/140‑1 (MQ=255)                                        gTTTACCACCACCACATCGGCGTCCATCGCTTTTTTACGTGCTTTGACCACAAAGCAATCTTTATACATCGGGCAGTCGCTGCCAAGACAGTTGTCGCTGGTGCTGGTGACCAGCGGCCACCCCTGTGAATCTTCCGCCa                                                                                              <  1:287732/140‑1 (MQ=255)                                          ttACCACCACCACATCGGCGTCCATCGCTTTTTTACGTGCTTTGACCACAAAGCAATCTTTATACATCGGGCAGTCGCTGCCAAGACAGTTGTCGCTGGTGCTGGTGACCAGCGGCCACGCCTGTGAATCTTCCGCCACg                                                                                            <  2:367726/140‑1 (MQ=255)                                            accaccaccACATCGGCGTCCATCGCTTTTTTATGTGCTTTGACCACAAAGCAATCTTTATACATCGGGCAGTCGCTGCCAAGACAGTTGTCGCTGGTGCTGGTGACCAGCGGCCACGCCTGTGAATCTTCCGCCACGCt                                                                                          >  1:683928/1‑140 (MQ=255)                                            accaccaccACATCGGCGTCCATCGCTTTTTTACGTGCTTTGACCACAAAGCAATCTTTATACATCGGGCAGTCGCTGCCAAGACAGTTGTCGCTGGTGCTGGTGACCAGCGGCCACGCCTGTGAATCTTCCGc                                                                                                <  1:909027/134‑1 (MQ=255)                                            accaccaccACATCGGCGTCCATCGCTTTTTTACGTGCTTTGACCACAAAGCAATCTTTATACATCGGGCAGTCGCTGCCAAGACAGTTGTCGCTGGTGCTGGTGACCAGCGGCCACGCCTGTGAATCTTCCGc                                                                                                >  2:909027/1‑134 (MQ=255)                                                 caccacATCGGCGTCCATCGCTTTTTTACGTGCTTTGACCACAAAGCAATCTTTATACATCGGGCAGTCGCTGCCAAGACAGTTGTCGCTGGTGCTGGTGACCAGCGGCCACGCCTGTGAATCTTCCGCCACGCTGACGc                                                                                     <  1:856836/140‑1 (MQ=255)                                                   ccacATCGGCGTCCATCGCTTTTTTACGTGCTTTGACCACAAAGCAATCTTTATACATCGGGCAGTCGCTGCCAAGACAGTTGTCGCTGGTGCTGGTGACCAGCGGCCACGCCTGTGAATCTTCCGCCACGCTGACGCAg                                                                                   >  2:249334/1‑140 (MQ=255)                                                   ccacATCGGCGTCCATCGCTTTTTTACGTGCTTTGACCACAAAGCAATCTTTATACATCGGGCAGTCGCTGCCAAGACAGTTGTCGCTGGTGCTGGTGACCAGCGGCCACGCCTGTGAATCTTCCGCCACGCTGACGCAg                                                                                   >  2:270778/1‑140 (MQ=255)                                                      caTCGGCGTCCATCGCTTTTTTACGTGCTTTGACCACAAAGCAATCTTTATACATCGGGCAGTCGCTGCCAAGACAGTTGTCGCTGGTGCTGGTGACCAGCGGCCACGCCTGTGAATCTTCCGCCACGCTGACGCAGGTg                                                                                <  2:662112/140‑1 (MQ=255)                                                              tCCATCGCTTTTTTACGTGCTTTGACCACAAAGAAATCTTTATACATCGGGCAGTCGCTGCCAAGACAGTTGTCGCTGGTGCTGGTGACCAGCGGCCACGCCTGTGAATCTTCCGCCACGCTGACGCAGGTGCTGATATc                                                                        >  2:324636/1‑140 (MQ=255)                                                                     cTTTTTTACGTGCTTTGACCACAAAGCAATCTTTATACATCGGGCAGTCGCTGCCAAGACAGTTGTCGCTGGTGCTGGTGACCAGCGGCCACGCCTGTGAATCTTCCGCCACGCTGACGCAGGTGCTGATATCACCATCg                                                                 <  1:270778/140‑1 (MQ=255)                                                                          ttACGTGCTTTGACCACAAAGCAATCTTTATACATCGGGCAGTCGCTGCCAAGACAGTTGTCGCTGGTGCTGGTGACCAGCGGCCACGCCTGTGAATCTTCCGCCACGCTGACGCAGGTGCTGATATCACCATCGACTGt                                                            <  1:759757/140‑1 (MQ=255)                                                                             cGTGCTTTGACCACAAAGCAATCTTTATACATCGGGCAGTCGCTGCCAAGACAGTTGTCGCTGGTGCTGGTGACCAGCGGCCACGCCTGTGAATCTTCCGCCACGCTGACGCAGGTGCTGATATCACCATCGACTGTTTg                                                         >  2:349859/1‑140 (MQ=255)                                                                               tGCTTTGACCACAAAGCAATCTTTATACATCGGGCAGTCGCTGCCAAGACAGTTGTCGCTGGTGCTGGTGACCAGCGGCCACGCCTGTGAATCTTCCGCCACGCTGACGCAGGTGCTGATATCACCATCGACTGTTTGAt                                                       <  1:249334/140‑1 (MQ=255)                                                                               tGCTTTGACCACAAAGCAATCTTTATACATCGGGCAGTCGCTGCCAAGACAGTTGTCGCTGGTGCTGGTGACCAGCGGCCACGCCTGTGAATCTTCCGCCACGCTGACGCAGGTGCTGATATCACCATCGACTGTTTGAt                                                       <  2:142465/140‑1 (MQ=255)                                                                               tGCTTTGACCACAAAGCAATCTTTATACATCGGGCAGTCGCTGCCAAGACAGTTGTCGCTGGTGCTGGTGACCAGCGGCCACGCCTGTGAATCTTCCGCCACGCTGACGCAGGTGCTGATATCACCATCGACTGTTTGAt                                                       <  1:39979/140‑1 (MQ=255)                                                                                  tttGACCACAAAGCAATCTTTATACATCGGGCAGTCGCTGCCAAGACAGTTGTCGCTGGTGCTGGTGACCAGCGGCCACGCCTGTGAATCTTCCGCCACGCTGACGCAGGTGCTGATATCACCATCGACTGTTTGATTAg                                                    <  1:508514/140‑1 (MQ=255)                                                                                        cacaAAGCAATCTTTATACATCGGGCAGTCGCTGCCAAGACAGTTGTCGCTGGTGCTGGTGACCAGCGGCCACGCCTGTGAATCTTCCGCCACGCTGACGCAGGTGCTGATATCACCATCGACTGTTTGATTAGACCAgg                                              <  1:349859/140‑1 (MQ=255)                                                                                           aaaGAAATCTTTATCCATCGGGCAGTCGCTGCCAAGACAGTTGTCGCTGGTGCTGGTGACCAGCGGCCACGCCTGTGAATCTTCCGCCACGCTGACGCAGGTGCTGATATCACCATCGACTGTTTGATTAGACCAGGAgc                                           <  1:324636/140‑1 (MQ=255)                                                                                               cAATCTTTATACATCGGGCAGTCGCTGCCAAGACAGTTGTCGCTGGTGCTGGTGACCAGCGGCCACGCCTGTGAATCTTCCGCCACGCTGACGCAGGTGCTGATATCACCATCGACTGTTTGATTAGACCAGGAGCgcag                                       <  2:683928/140‑1 (MQ=255)                                                                                                            tCGGGCAGTCGCTGCCAAGACAGTTGTCGCTGGTGCTGGTGACCAGCGGCCACGCCTGTGAATCTTCCGCCACGCTGACGCAGGTGCTGATATCACCATCGACTGTTTGATTAGACCAGGAGCgcag                                       <  2:111509/127‑1 (MQ=255)                                                                                                            tCGGGCAGTCGCTGCCAAGACAGTTGTCGCTGGTGCTGGTGACCAGCGGCCACGCCTGTGAATCTTCCGCCACGCTGACGCAGGTGCTGATATCACCATCGACTGTTTGATTAGACCAGGAGCgcag                                       >  1:111509/1‑127 (MQ=255)                                                                                                            tCGGGCAGTCGCTGCCAAGACAGTTGTCGCTGGTGCTGGTGACCAGCGGCCACGCCTGTGAATCTTCCGCCACGCTGACGCAGGTGCTGATATCACCATCGACTGTTTGATTAGACCAGGAGCGCAGCAGGATCACATCg                          >  1:310147/1‑140 (MQ=255)                                                                                                            tCGGGCAGTCGCTGCCAAGACAGTTGTCGCTGGTGCTGGTGACCAGCGGCCACGCCTGTGAATCTTCCGCCACGCTGACGCAGGTGCTGATATCACCATCGACTGTTTGATTAGACCAGGAGCGCAGCAGGATCACATCg                          >  1:554305/1‑140 (MQ=255)                                                                                                             cGGGCAGTCGCTGCCAAGACAGTTGTCGCTGGTGCTGGTGACCAGCGGCCACGCCTGTGAATCTTCCGCCACGCTGACGCAGGTGCTGATATCACCATCGACTGTTTGATTAGACCAGGAGCGCAGCAGGATcac                              <  1:949862/135‑1 (MQ=255)                                                                                                             cGGGCAGTCGCTGCCAAGACAGTTGTCGCTGGTGCTGGTGACCAGCGGCCACGCCTGTGAATCTTCCGCCACGCTGACGCAGGTGCTGATATCACCATCGACTGTTTGATTAGACCAGGAGCGCAGCAGGATcac                              >  2:949862/1‑135 (MQ=255)                                                                                                             cGGGCAGTCGCTGCCAAGACAGTTGTCGCTGGTGCTGGTGACCAGCGGCCACGCCTGTGAATCTTCCGCCACGCTGACGCAGGTGCTGATATCACCATCGACTGTTTGATTAGACCAGGAGCGCAGCAGGATCACATCGc                         >  2:81175/1‑140 (MQ=255)                                                                                                             cGGGCAGTCGCTGCCAAGACAGTTGTCGCTGGTGCTGGTGACCAGCGGCCACGCCTGTGAATCTTCCGCCACGCTGACGCAGGTGCTGATATCACCATCGACTGTTTGATTAGACCAGGAGCGCAGCAGGATCACATCGc                         >  2:91988/1‑140 (MQ=255)                                                                                                             cGGGCAGTCGCTGCCAAGACAGTTGTCGCTGGTGCTGGTGACCAGCGGCCACGCCTGTGAATCTTCCGCCACGCTGACGCAGGTGCTGATATCACCATCGACTGTTTGATTAGACCAGGAGCGCAGCAGGATCACATCGc                         >  1:207150/1‑140 (MQ=255)                                                                                                                gCAGTCGCTGCCAAGACAGTTGTCGCTGGTGCTGGTGACCAGCGGCCACGCCTGTGAATCTTCCGCCACGCTGACGCAGGTGCTGATATCACCATCGACTGTTTGATTAGACCAGGAGCGCAGCAGGATCACATCGCTTa                      >  2:467545/1‑140 (MQ=255)                                                                                                                              gACAGTTGCCGCTGGTGCTGGTGACCAGCGGCCACGCCTGTGAATCTTCCGCCACGCTGACGCAGGTGCTGATATCACCATCGACTGTTTGATTAGACCAGGAGCGCAGCAGGATCACATCGCTTAAGATTTGTACCGGc        <  1:716426/140‑1 (MQ=255)                                                                                                                                  gTTGTCGCTGGTGCTGGTGACCAGCGGCCACGCCTGTGAATCTTCCGCCACGCTGACGCAGGTGCTGATATCACCATCGACTGTTTGATTAGACCAGGAGCGCAGCAGGATCACATCGCTTAAGATTTGTACCGGCAGAt    <  2:207150/140‑1 (MQ=255)                                                                                                                                    tGTCGCTGGTGCTGGTGACCAGCGGCCACGCCTGTGAATCTTCCGCCACGCTGACGCAGGTGCTGATATCACCATCGACTGTTTGATTAGACCAGGAGCGCAGCAGGATCACATCGCTTAAGATTTGTACCGGCAGATCg  >  1:846197/1‑140 (MQ=255)                                                                                                                                         |                                                                                                                                       ACTCTCTTTAACCACCATATCCGCCAGAAAGAGATGATGGTTTACCACCACCACATCGGCGTCCATCGCTTTTTTACGTGCTTTGACCACAAAGCAATCTTTATACATCGGGCAGTCGCTGCCAAGACAGTTGTCGTTGGTGCTGGTGACCAGCGGCCACGCCTGTGAATCTTCCGCCACGCTGACGCAGGTGCTGATATCACCATCGACTGTTTGATTAGACCAGGAGCGCAGCAGGATCACATCGCTTAAGATTTGTACCGGCAGATCG  >  NC_000913/1892606‑1892876

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑