Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,046,454	T→C	V506A (GTC→GCC)	yeeJ →	inverse autotransporter adhesin

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,046,454	0	T	C	100.0%	185.6 / NA	53	V506A (GTC→GCC)	yeeJ	inverse autotransporter adhesin
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (29/24);  total (29/24)

GGCTATAACGTCGAAGCCACCGCACTGGAAGCTGCCGGTGGCAAAGTGGTCACAACGGGTAAAGATATTCTGGTTACCCTGCCGGCTTACCGGTTCACCAGTACGCCAGAAACCGATAACACCTGGCCGATTGAAGTCACCGCCGAAGATGTCAAAGGCAATTTGTCGAATCGTGAACAGAGCATGGTGGTCGTTCAGGCACCTACGCTAAGCCAGAAAGATTCCTCGGTATCGTTAAGTACCCAAACATTGAACGCGGATTCCCATTCA  >  NC_000913/2046318‑2046587                                                                                                                                         |                                                                                                                                      ggCTATAACGTCGAAGCCACCGCACTGGAAGCTGCCGGTGGCAAAGTGGTCACAACGGGTAAAGATATTCTGGTTACCCTGCCGGCTTACCGGTTCACCAGTACGCCAGAAACCGATAACACCTGGCCGATTGAAGCCAc                                                                                                                                    <  2:105920/140‑1 (MQ=255)           tCGAAGCCACCGCACTGGAAGCTGCCGGTGGCAAAGTGGTCACAACGGGTAAAGATATTCTGGTTACCCTGCCGGCTTACCGGTTCACCAGTACGCCAGAAACCGATAACACCTGGCCGATTGAAGCCACCGCCGAAGAt                                                                                                                          >  1:333323/1‑140 (MQ=255)                  cACCGCACTGGAAGCTGCCGGTGGCAAAGTGGTCACAACGGGTAAAGATATTCTGGTTACCCTGCCGGCTTACCGGTTCACCAGTACGCCAGAAACCGATAACACCTGGCCGATTGAAGCCACCGCCGAAGATGTCAAAg                                                                                                                   >  2:544828/1‑140 (MQ=255)                    ccGCACTGGAAGCTGCCGGTGGCAAAGTGGTCACAACGGGTAAAGATATTCTGGTTACCCTGCCGGCTTACCGGTTCACCAGTACGCCAGAAACCGATAACACCTGGCCGATTGAAGCCACCGCCGAAGATGTCaa                                                                                                                     >  2:336080/1‑136 (MQ=255)                    ccGCACTGGAAGCTGCCGGTGGCAAAGTGGTCACAACGGGTAAAGATATTCTGGTTACCCTGCCGGCTTACCGGTTCACCAGTACGCCAGAAACCGATAACACCTGGCCGATTGAAGCCACCGCCGAAGATGTCaa                                                                                                                     <  1:336080/136‑1 (MQ=255)                            gAAGCTGCCGGTGGCAAAGTGGTCACAACGGGTAAAGATATTCTGGTTACCCTGCCGGCTTACCGGTTCACCAGTACGCCAGAAACCGATAACACCTGGCCGATTGAAGCCACCGCCGAAGATGTCAAAGGCAATTTGTc                                                                                                         <  1:764151/140‑1 (MQ=255)                                 tGCCGGTGGCAAAGTGGTCACAACGGGTAAAGATATTCTGGTTACCCTGCCGGCTTACCGGTTCACCAGTACGCCAGAAACCGATAACACCTGGCCGATTGAAGCCACCGCCGAAGATGTCAAAGGCAATTTGTCGAATc                                                                                                    <  2:716580/140‑1 (MQ=255)                                   ccGGTGGCAAAGTGGTCACAACGGGTAAAGATATTCTGGTTACCCTGCCGGCTTACCGGTTCACCAGTACGCCAGAAACCGATAACACCTGGCCGATTGAAGCCACCGCCGAAGATGTCAAAGGCAATTTGTCGAATCGt                                                                                                  <  1:629328/140‑1 (MQ=255)                                    cGGTGGCAAAGTGGTCACAACGGGTAAAGATATTCTGGTTACCCTGCCGGCTTACCGGTTCACCAGTACGCCAGAAACCGATAACACCTGGCCGATTGAAGCCACCGCCGAAGATGTCAAAGGCAATTTGTCGAATCGTg                                                                                                 >  2:918554/1‑140 (MQ=255)                                      gTGGCAAAGTGGTCACAACGGGTAAAGATATTCTGGTTACCCTGCCGGCTTACCGGTTCACCAGTACGCCAGAAACCGATAACACCTGGCCGATTGAAGCCACCGCCGAAGATGTCAAAGGCAATTTGTCGAATCGTGaa                                                                                               >  1:120573/1‑140 (MQ=255)                                                   cacaACGGGTAAAGATATTCTGGTTACCCTGCCGGCTTACCGGTTCACCAGTACGCCAGAAACCGATAACACCTGGCCGATTGAAGCCACCGCCGAAGATGTCAAAGGCAATTTGTCGAATCGTGAACAGAGCAtggtgg                                                                                  >  2:855538/1‑140 (MQ=255)                                                   cacaACGGGTAAAGATATTCTGGTTACCCTGCCGGCTTACCGGTTCACCAGTACGCCAGAAACCGATAACACCTGGCCGATTGAAGCCACCGCCGAAGATGTCAAAGGCAATTTGTCGAATCGTGAACAGAGCAtggtgg                                                                                  >  1:940586/1‑140 (MQ=255)                                                   cacaACGGGTAAAGATATTCTGGTTACCCTGCCGGCTTACCGGTTCACCAGTACGCCAGAAACCGATAACACCTGGCCGATTGAAGCCACCGCCGAAGATGTCAAAGGCAATTTGTCGAATCGTGAACAGAGCAtggtgg                                                                                  <  1:855538/140‑1 (MQ=255)                                                    acaACGGGTAAAGATATTCTGGTTACCCTGCCGGCTTACCGGTTCACCAGTACGCCAGAAACCGATAACACCTGGCCGATTGAAGCCACCGCCGAAGATGTCAAAGGCAATTTGTCGAATCGTGAACAGAGCATggtggt                                                                                 <  2:333323/140‑1 (MQ=255)                                                    acaACGGGTAAAGATATTCTGGTTACCCTGCCGGCTTACCGGTTCACCAGTACGCCAGAAACCGATAACACCTGGCCGATTGAAGCCACCGCCGAAGATGTCAAAGGCAATTTGTCGAATCGTGAACAGAGCATggtggt                                                                                 <  1:918554/140‑1 (MQ=255)                                                                gATATTCTGGTTACCCTGCCGGCTTACCGGTTCACCAGTACGCCAGAAACCGATAACACCTGGCCGATTGAAGCCACCGCCGAAGATGTCAAAGGCAATTTGTCGAATCGTGAACAGAGCATGGTGGTCGTTCAGGCAcc                                                                     <  2:967044/140‑1 (MQ=255)                                                                gATATTCTGGTTACCCTGCCGGCTTACCGGTTCACCAGTACGCCAGAAACCGATAACACCTGGCCGATTGAAGCCACCGCCGAAGATGTCAAAGGCAATTTGTCGAATCGTGAACAGAGCATGGTGGTCGTTCAGGCAcc                                                                     <  1:544828/140‑1 (MQ=255)                                                                gATATTCTGGTTACCCTGCCGGCTTACCGGTTCACCAGTACGCCAGAAACCGATAACACCTGGCCGATTGAAGCCACCGCCGAAGATGTCAAAGGCAATTTGTCGAATCGTGAACAGAGCATGGTGGTCGTTCAGGCAcc                                                                     <  1:618468/140‑1 (MQ=255)                                                                gATATTCTGGTTACCCTGCCGGCTTACCGGTTCACCAGTACGCCAGAAACCGATAACACCTGGCCGATTGAAGCCACCGCCGAAGATGTCAAAGGCAATTTGTCGAATCGTGAACAGAGCATGGTGGTCGTTCAGGCAcc                                                                     <  1:480786/140‑1 (MQ=255)                                                                    ttCTGGTTACCCTGCCGGCTTACCGGTTCACCAGTACGCCAGAAACCGATAACACCTGGCCGATTGAAGCCACCGCCGAAGATGTCAAAGGCAATTTGTCGAATCGTGAACAGAGCATGGTGGTCGTTCAGGCACCTACg                                                                 >  2:902184/1‑140 (MQ=255)                                                                    ttCTGGTTACCCTGCCGGCTTACCGGTTCACCAGTACGCCAGAAACCGATAACACCTGGCCGATTGAAGCCACCGCCGAAGATGTCAAAGGCAATTTGTCGAATCGTGAACAGAGCATGGTGGTCGTTCAGGCACCTACg                                                                 >  1:763077/1‑140 (MQ=255)                                                                      cTGGTTACCCTGCCGGCTTACCGGTTCACCAGTACGCCAGAAACCGATAACCCCTGGCCGATTGAAGCCACCGCCGAAGATGTCAAAGGCAATTTGTCGAATCGTGAACAGAGCATGGTGGTCGTTCAGGCACCTACGCt                                                               <  2:191445/140‑1 (MQ=255)                                                                      cTGGTTACCCTGCCGGCTTACCGGTTCACCAGTACGCCAGAAACCGATAACACCTGGCCGATTGAAGCCACCGCCGAAGATGTCAAAGGCAATTTGTCGAATCGTGAACAGAGCATGGTGGTCGTTCAGGCACCTACGCt                                                               <  2:120573/140‑1 (MQ=255)                                                                      cTGGTTACCCTGCCGGCTTACCGGTTCACCAGTACGCCAGAAACCGATAACACCTGGCCGATTGAAGCCACCGCCGAAGATGTCAAAGGCAATTTGTCGAATCGTGAACAGAGCATGGTGGTCGTTCAGGCACCTACGCt                                                               <  1:902184/140‑1 (MQ=255)                                                                         gTTACCCTGCCGGCTTACCGGTTCACCAGTACGCCAGAAACCGATAACACCTGGCCGATTGAAGCCACCGCCGAAGATGTCAAAGGCAATTTGTCGAATCGTGAACAGAGCATGGTGGTCGTTCAGGCACCTACGCTAAg                                                            >  1:730497/1‑140 (MQ=255)                                                                         gTTACCCTGCCGGCTTACCGGTTCACCAGTACGCCAGAAACCGATAACACCTGGCCGATTGAAGCCACCGCCGAAGATGTCAAAGGCAATTTGTCGAATCGTGAACAGAGCATGGTGGTCGTTCAGGCACCTACGCTAAg                                                            >  1:724975/1‑140 (MQ=255)                                                                         gTTACCCTGCCGGCTTACCGGTTCACCAGTACGCCAGAAACCGATAACACCTGGCCGATTGAAGCCACCGCCGAAGATGTCAAAGGCAATTTGTCGAATCGTGAACAGAGCATGGTGGTCGTTCAGGCACCTACGCTAAg                                                            >  1:701719/1‑140 (MQ=255)                                                                          ttACCCTGCCGGCTTACCGGTTCACCAGTACGCCAGAAACCGATAACACCTGGCCGATTGAAGCCACCGCCGAAGATGTCAAAGGCAATTTGTCGAATCGTGAACAGAGCATGGTGGTCGTTCAGGCACCTACGCTAAGc                                                           >  2:317173/1‑140 (MQ=255)                                                                          ttACCCTGCCGGCTTACCGGTTCACCAGTACGCCAGAAACCGATAACACCTGGCCGATTGAAGCCACCGCCGAAGATGTCAAAGGCAATTTGTCGAATCGTGAACAGAGCATGGTGGTCGTTCAGGCACCTACGCTAAGc                                                           >  1:62683/1‑140 (MQ=255)                                                                           tACCCTGCCGGCTTACCGGTTCACCAGTACGCCAGAAACCGATAACACCTGGCCGATTGAAGCCACCGCCGAAGATGTCAAAGGCAATTTGTCGAATCGTTAACAGAGCATGGTGGTCGTTCAGGCACCTACGCTAAGcc                                                          >  1:933785/1‑140 (MQ=255)                                                                           tACCCTGCCGGCTTACCGGTTCACCAGTACGCCAGAAACCGATAACACCTGGCCGATTGAAGCCACCGCCGAAGATGTCAAAGGCAATTTGTCGAATCGTGAACAGAGCATGGTGGTCGTTCAGGCACCTACGCTAAGcc                                                          >  1:308367/1‑140 (MQ=255)                                                                            aCCCTGCCGGCTTACCGGTTCACCAGTACGCCAGAAACCGATAACACCTGGCCGATTGAAGCCACCGCCGAAGATGTCAAAGGCAATTTGTCGAATCGTGAACAGAGCATGGTGGTCGTTCAGGCACCTACGCTAAGCCa                                                         >  1:838232/1‑140 (MQ=255)                                                                              ccTGCCGGCTTACCGGTTCACCAGTACGCCAGAAACCGATAACACCTGGCCGATTGAAGCCACCGCCGAAGATGTCAAAGGCAATTTGTCGAATCGTGAACAGAGCATGGTGGTCGTTCAGGCACCTACGCTAAGCCAGa                                                       >  1:226197/1‑140 (MQ=255)                                                                                 gCCGGCTTACCGGTCCACCAGTACGCCAGAAACCGATAACACCTGGCCGATTGAAGCCACCGCCGAAGATGTCAAAGGCAATTTGTCGAATCGTGAACAGAGCATGGTGGTCGTTCAGGCAcc                                                                     >  1:660026/1‑123 (MQ=255)                                                                                 gCCGGCTTACCGGTCCACCAGTACGCCAGAAACCGATAACACCTGGCCGATTGAAGCCACCGCCGAAGATGTCAAAGGCAATTTGTCGAATCGTGAACAGAGCATGGTGGTCGTTCAGGCAcc                                                                     <  2:660026/123‑1 (MQ=255)                                                                                  ccGGCTTACCGGTTCACCAGTACGCCAGAAACCGATAACACCTGGCCGATTGAAGCCACCGCCGAAGATGTCAAAGGCAATTTGTCGAATCGTGAACAGAGCATGGTGGTCGTTCAGGCACCTACGCTAAGCCAGAAAGa                                                   <  2:243972/140‑1 (MQ=255)                                                                                  ccGGCTTACCGGTTCACCAGTACGCCAGAAACCGATAACACCTGGCCGATTGAAGCCACCGCCGAAGATGTCAAAGGCAATTTGTCGAATCGTGAACAGAGCATGGTGGTCGTTCAGGCACCTACGCTAAGCCAGAAAGa                                                   >  2:817713/1‑140 (MQ=255)                                                                                   cGGCTTACCGGTTCACCAGTACGCCAGAAACCGATAACACCTGGCCGATTGAAGCCACCGCCGAAGATGTCAAAGGCAATTTGTCGAATCGTGAACAGAGCATGGTGGTCGTTCAGGCACCTACGCTAAGCCAGAAAGAt                                                  >  1:537100/1‑140 (MQ=255)                                                                                       ttACCGGTTCACCAGTACGCCAGAAACCGATAACACCTGGCCGATTGAAGCCACCGCCGAAGATGTCAAAGGCAATTTGTCGAATCGTGAACAGAGCATGGTGGTCGTTCAGGCACCTACGCTAAGCCAGAAAGATTCCt                                              <  2:308367/140‑1 (MQ=255)                                                                                       ttACCGGTTCACCAGTACGCCAGAAACCGATAACACCTGGCCGATTGAAGCCACCGCCGAAGATGTCAAAGGCAATTTGTCGAATCGTGAACAGAGCATGGTGGTCGTTCAGGCACCTACGCTAAGCCAGAAAGATTCCt                                              <  2:724975/140‑1 (MQ=255)                                                                                            ggTTCACCAGTACGCCAGAAACCGATAACACCTGGCCGATTGAAGCCACCGCCGAAGATGTCAAAGGCAATTTGTCGAATCGTGAACAGAGCATGGTGGTCGTTCAGGCACCTACGCTAAGCCAGAAAGATTCCTCGGTa                                         <  1:817713/140‑1 (MQ=255)                                                                                            ggTTCACCAGTACGCCAGAAACCGATAACACCTGGCCGATTGAAGCCACCGCCGAAGATGTCAAAGGCAATTTGTCGAATCGTGAACAGAGCATGGTGGTCGTTCAGGCACCTACGCTAAGCCAGAAAGATTCCTCGGTa                                         >  1:311104/1‑140 (MQ=255)                                                                                             gTTCACCAGTACGCCAGAAACCGATAACACCTGGCCGATTGAAGCCACCGCCGAAGATGTCAAAGGCAATTTGTCGAATCGTGAACAGAGCATGGTGGTCGTTCAGGCACCTACGCTAAGCCAGAAAGATTCCTCGGTAt                                        <  2:730497/140‑1 (MQ=255)                                                                                                   cAGTACGCCAGAAACCGATAACACCTGGCCGATTGAAGCCACCGCCGAAGATGTCAAAGGCAATTTGTCGAATCGTGAACAGAGCATGGTGGTCGTTCAGGCACCTACGCTAAGCCAGAAAGATTCCTCGGTa                                         >  2:413979/1‑133 (MQ=255)                                                                                                   cAGTACGCCAGAAACCGATAACACCTGGCCGATTGAAGCCACCGCCGAAGATGTCAAAGGCAATTTGTCGAATCGTGAACAGAGCATGGTGGTCGTTCAGGCACCTACGCTAAGCCAGAAAGATTCCTCGGTa                                         <  1:413979/133‑1 (MQ=255)                                                                                                    aGTACGCCAGAAACCGATAACACCTGGCCGATTGAAGCCACCGCCGAAGATGTCAAAGGCAATTTGTCGAATCGTGAACAGAGCATGGTGGTCGTTCAGGCACCTACGCTAAGCCAGAAAGATTCCTCGGTATCGTTAAg                                 <  1:317173/140‑1 (MQ=255)                                                                                                        cGCCAGAAACCGATAACACCTGGCCGATTGAAGCCACCGCCGAAGATGTCAAAGGCAATTTGTCGAATCGTGAACAGAGCATGGTGGTCGTTCAGGCACCTACGCTAAGCCAGAAAGATTCCTCg                                            <  2:647639/125‑1 (MQ=255)                                                                                                        cGCCAGAAACCGATAACACCTGGCCGATTGAAGCCACCGCCGAAGATGTCAAAGGCAATTTGTCGAATCGTGAACAGAGCATGGTGGTCGTTCAGGCACCTACGCTAAGCCAGAAAGATTCCTCg                                            >  1:647639/1‑125 (MQ=255)                                                                                                        cGCCAGAAACCGATAACACCTGGCCGATTGAAGCCACCGCCGAAGATGTCAAAGGCAATTTGTCGAATCGTGAACAGAGCATGGTGGTCGTTCAGGCACCTACGCTAAGCCAGAAAGATTCCTCGGTATCGTTAAGTAcc                             >  1:855194/1‑140 (MQ=255)                                                                                                                              gCCGATTGAAGCCACCGCCGAAGATGTCAAAGGCAATTTGTCGAATCGTGAACAGAGCATGGTGGTCGTTCAGGCACCTACGCTAAGCCAGAAAGATTCCTCGGTATCGTTAAGTACCCAAACATTGAACGCGGATTccc       >  1:703235/1‑140 (MQ=255)                                                                                                                                   ttGAAGCCACCGCCGAAGATGTCAAAGGCAATTTGTCGAATCGTGAACAGAGCATGGTGGTCGTTCAGGCACCTACGCTAAGCCAGAAAGATTCCTCGGTATCGTTAAGTACCCAAACATTGAACGTGGATTCCCATTCa  >  2:557313/1‑140 (MQ=255)                                                                                                                                   ttGAAGCCACCGCCGAAGATGTCAAAGGCAATTTGTCGAATCGTGAACAGAGCATGGTGGTCGTTCAGGCACCTACGCTAAGCCAGAAAGATTCCTCGGTATCGTTAAGTACCCAAACATTGAACGCGGATTCCCATTCa  >  1:42851/1‑140 (MQ=255)                                                                                                                                   ttGAAGCCACCGCCGAAGATGTCAAAGGCAATTTGTCGAATCGTGAACAGAGCATGGTGGTCGTTCAGGCACCTACGCTAAGCCAGAAAGATTCCTCGGTATCGTTAAGTACCCAAACATTGAACGCGGATTCCCATTCa  >  1:256699/1‑140 (MQ=255)                                                                                                                                         |                                                                                                                                      GGCTATAACGTCGAAGCCACCGCACTGGAAGCTGCCGGTGGCAAAGTGGTCACAACGGGTAAAGATATTCTGGTTACCCTGCCGGCTTACCGGTTCACCAGTACGCCAGAAACCGATAACACCTGGCCGATTGAAGTCACCGCCGAAGATGTCAAAGGCAATTTGTCGAATCGTGAACAGAGCATGGTGGTCGTTCAGGCACCTACGCTAAGCCAGAAAGATTCCTCGGTATCGTTAAGTACCCAAACATTGAACGCGGATTCCCATTCA  >  NC_000913/2046318‑2046587

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑