Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,058,589	C→T	A420T (GCT→ACT)	yeeO ←	FMN/FAD exporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,058,589	0	C	T	100.0%	157.4 / NA	46	A420T (GCT→ACT)	yeeO	FMN/FAD exporter
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (24/22);  total (24/22)

TTTAAATCCAGCGGGTAGCACCCATGAGGCGGACCAAATAGGCATAAATAAAGCATTTAGCCAAATCAGAATCACAACGACATGTTTAACCTGTGGATCCTGGGTGTAAAACGATGCCATAACCCCGGCAAAGGGAGCCGTTAGCCAGGCGATGGCCGTTAATCCAAGAGTGGAAAGCCAGAACACATGCCGCAACTGAATCTCTGCTTGCGCTATCTGCCCTACCCCCAACCTTCGGCCTGTAATGATCGTAGAAGCAGAGCCGAGCG  >  NC_000913/2058452‑2058720                                                                                                                                          |                                                                                                                                    tttAAATCCAGCGGGTAGCACCCATGAGGCGGACCAAATAGGCATAAATAAAGCATTTAGCCAAATCAGAATCACAACGACATGTTTAACCTGTGGATCCTGGGTGTAAAACGATGCCATAACCCCGGCAAAGGGAGTCg                                                                                                                                   >  1:736877/1‑140 (MQ=255)          aGCGGGTAGCACCCATGAGGCGGACCAAATAGGCATAAATAAAGCATTTAGCCAAATCAGAATCACAACGACATGTTTAACCTGTGGATCCTGGGTGTAAAACGATGCCATAACCCCGGCAAAGGGAGTCGTTAGCCAgg                                                                                                                          >  2:621225/1‑140 (MQ=255)          aGCGGGTAGCACCCATGAGGCGGACCAAATAGGCATAAATAAAGCATTTAGCCAAATCAGAATCACAACGACATGTTTAACCTGTGGATCCTGGGTGTAAAACGATGCCATAACCCCGGCAAAGGGAGTCGTTAGCCAgg                                                                                                                          >  1:945879/1‑140 (MQ=255)                tAGCACCCATGAGGCGGACCAAATAGGCATAAATAAAGCATTTAGCCAAATCAGAATCACAACGACATGTTTAACCTGTGGATCCTGGGTGTAAAACGATGCCATAACCCCGGCAAAGGGAGTCGTTAGCCAGGCGATgg                                                                                                                    <  1:295594/140‑1 (MQ=255)                  gCACCCATGAGGCGGACCAAATAGGCATAAATAAAGCATTTAGCCAAATCAGAATCACAACGACATGTTTAACCTGTGGATCCTGGGTGTAAAACGATGCCATAACCCCGGCAAAGGGAGTCGTTAGCCAGGCGATGGcc                                                                                                                  <  2:848928/140‑1 (MQ=255)                    aCCCATGAGGCGGACCAAATAGGCATAAATAAAGCATTTAGCCAAATCAGAATCACAACGACATGTTTAACCTGTGGATCCTGGGTGTAAAACGATGCCATAACCCCGGCAAAGGGAGTCGTTAGCCAGGCGATGGCCGt                                                                                                                <  2:883768/140‑1 (MQ=255)                       cATGAGGCGGACCAAATAGGCATAAATAAAGCATTTAGCCAAATCAGAATCACAACGACATGTTTAACCTGTGGATCCTGGGTGTAAAACGATGCCATAACCCCGGCAAAGGGAGTCGTTAGCCAGGCGATGGCCGTTaa                                                                                                             <  2:736877/140‑1 (MQ=255)                             gCGGACCAAATAGGCATAAATAAAGCATTTAGCCAAATCAGAATCACAACGACATGTTTAACCTGTGGATCCTGGGTGTAAAACGATGCCATAACCCCGGCAAAGGGAGTCGTTAGCCAGGCGATGGCCGTTAATCCAag                                                                                                       <  1:305147/140‑1 (MQ=255)                                gACCAAATAGGCATAAATAAAGCATTTAGCCAAATCAGAATCACAACGACATGTTTAACCTGTGGATCCTGGGTGTAAAACGATGCCATAACCCCGGCAAAGGGAGTCGTTAGCCAGGCGATGGCCGTTAATCCAAGAGt                                                                                                    >  2:310125/1‑140 (MQ=255)                                    aaaTAGGCATAAATAAAGCATTTAGCCAAATCAGAATCACAACGACATGTTTAACCTGTGGATCCTGGGTGTAAAACGATGCCATAACCCCGGCAAAGGGAGTCGTTAGCCAGGCGATGGCCGTTAATCCAAGAGTGGaa                                                                                                <  1:551287/140‑1 (MQ=255)                                           cATAAATAAAGCATTTAGCCAAATCAGAATCACAACGACATGTTTAACCTGTGGATCCTGGGTGTAAAACGATGCCATAACCCCGGCAAAGGGAGTCGTTAGCCAGGCGATGGCCGTTAATCCAAGAGTGGAAAGCCAGa                                                                                         >  1:525905/1‑140 (MQ=255)                                           cATAAATAAAGCATTTAGCCAAATCAGAATCACAACGACATGTTTAACCTGTGGATCCTGGGTGTAAAACGATGCCATAACCCCGGCAAAGGGAGTCGTTAGCCAGGCGATGGCCGTTAATCCAAGAGTGGAAAGCCAGa                                                                                         >  1:597678/1‑140 (MQ=255)                                           cATAAATAAAGCATTTAGCCAAATCAGAATCACAACGACATGTTTAACCTGTGGATCCTGGGTGTAAAACGATGCCATAACCCCGGCAAAGGGAGTCGTTAGCCAGGCGATGGCCGTTAATCCAAGAGTGGAAAGCCAGa                                                                                         >  2:52120/1‑140 (MQ=255)                                           cATAAATAAAGCATTTAGCCAAATCAGAATCACAACGACATGTTTAACCTGTGGATCCTGGGTGTAAAACGATGCCATAACCCCGGCAAAGGGAGTCGTTAGCCAGGCGATGGCCGTTAATCCAAGAGTGGAAAGCCAGa                                                                                         >  1:339471/1‑140 (MQ=255)                                                ataaaGCATTTAGCCAAATCAGAATCACAACGACATGTTTAACCTGTGGATCCTGGGTGTAAAACGATGCCATAACCCCGGCAAAGGGAGTCGTTAGCCAGGCGATGGCCGTTAATCCAAGAGTGGAAAGCCAGAAcaca                                                                                    <  1:320844/140‑1 (MQ=255)                                                    aGCATTTAGCCAAATCAGAATCACAACGACATGTTTAACCTGTGGATCCTGGGTGTAAAACGATGCCATAACCCCGGCAAAGGGAGTCGTTAGCCAGGCGATGGCCGTTAATCCAAGAGTGGAAAGCCAGAACACATGcc                                                                                >  1:71123/1‑140 (MQ=255)                                                    aGCATTTAGCCAAATCAGAATCACAACGACATGTTTAACCTGTGGATCCTGGGTGTAAAACGATGCCATAACCCCGGCAAAGGGAGTCGTTAGCCAGGCGATGGCCGTTAATCCAAGAGTGGAAAGCCAGAACACATGcc                                                                                <  2:273060/140‑1 (MQ=255)                                                        tttAGCCAAATCAGAATCACAACGACATGTTTAACCTGTGGATCCTGGGTGTAAAACGATGCCATAACCCCGGCAAAGGGAGTCGTTAGCCAGGCGATGGCCGTTAATCCAAGAGTGGAAAGCCAGAACACATGCCGCaa                                                                            <  2:339471/140‑1 (MQ=255)                                                         ttAGCCAAATCAGAATCACAACGACATGTTTAACCTGTGGATCCTGGGTGTAAAACGATGCCATAACCCCGGCAAAGGGAGTCGTTAGCCAGGCGATGGCCGTTAATCCAAGAGTGGAAAGCCAGAACACATGCCGCAAc                                                                           >  1:450627/1‑140 (MQ=255)                                                          tAGCCAAATCAGAATCACAACGACATGTTTAACCTGTGGATCCTGGGTGTAAAACGATGCCATAACCCCGGCAAAGGGAGTCGTTAGCCAGGCGATGGCCGTTAATCCAAGAGTGGAAAGCCAGAACACATGCCGCAACt                                                                          <  1:621225/140‑1 (MQ=255)                                                           aGCCAAATCAGAATCACAACGACATGTTTAACCTGTGGATCCTGGGTGTAAAACGATGCCATAACCCCGGCAAAGGGAGTCGTTAGCCAGGCGATGGCCGTTAATCCAAGAGTGGAAAGCCAGAACACATGCCGCAACTg                                                                         >  2:114586/1‑140 (MQ=255)                                                                aaTCAGAATCACAACGACATGTTTAACCTGTGGATCCTGGGTGTAAAACGATGCCATAACCCCGGCAAAGGGAGTCGTTAGCCAGGCGATGGCCGTTAATCCAAGAGTGGAAAGCCAGAACACATGCCGCAACTGAAtct                                                                    <  1:52120/140‑1 (MQ=255)                                                                      aaTCACAACGACATGTTTAACCTGTGGATCCTGGGTGTAAAACGATGCCATAACCCCGGCAAAGGGAGTCGTTAGCCAGGCGATGGCCGTTAATCCAAGAGTGGAAAGCCAGAACACATGCCGCAACTGAATCTCTGCtt                                                              >  2:25025/1‑140 (MQ=255)                                                                       aTCACAACGACATGTTTAACCTGTGGATCCTGGGTGTAAAACGATGCCATAACCCCGGCAAAGGGAGTCGTTAGCCAGGCGATGGCCGTTAATCCAAGAGTGGAAAGCCAGAACACATGCCGCAACTGAATCTCTGCTTg                                                             <  1:25025/140‑1 (MQ=255)                                                                       aTCACAACGACATGTTTAACCTGTGGATCCTGGGTGTAAAACGATGCCATAACCCCGGCAAAGGGAGTCGTTAGCCAGGCGATGGCCGTTAATCCAAGAGTGGAAAGCCAGAACACATGCCGCAACTGAATCTCTGCTTg                                                             <  2:123836/140‑1 (MQ=255)                                                                            aaCGACATGTTTAACCTGTGGATCCTGGGTGTAAAACGATGCCATAACCCCGGCAAAGGGAGTCGTTAGCCAGGCGATGGCCGTTAATCCAAGAGTGGAAAGCCAGAACACATGCCGCAACTGAATCTCTGCtt                                                              >  2:309071/1‑134 (MQ=255)                                                                            aaCGACATGTTTAACCTGTGGATCCTGGGTGTAAAACGATGCCATAACCCCGGCAAAGGGAGTCGTTAGCCAGGCGATGGCCGTTAATCCAAGAGTGGAAAGCCAGAACACATGCCGCAACTGAATCTCTGCtt                                                              <  1:309071/134‑1 (MQ=255)                                                                                aCATGTTTAACCTGTGGATCCTGGGTGTAAAACGATGCCATAACCCCGGCAAAGGGAGTCGTTAGCCAGGCGATGGCCGTTAATCCAAGAGTGGAAAGCCAGAACACATGCCGCAACTGAATCTCTGCTTGCGCTATCTg                                                    >  2:285002/1‑140 (MQ=255)                                                                                          ccTGTGGATCCTGGGTGTAAAACGATGCCATAACCCCGGCAAAGGGAGTCGTTAGCCAGGCGATGGCCGTTAATCCAAGAGTGGAAAGCCAGAACACATGCCGCAACTGAATCTCTGCTTGCGCTATCTGCCCTAccccc                                          >  2:915244/1‑140 (MQ=255)                                                                                              tgGATCCTGGGTGTAAAACGATGCCATAACCCCGGC‑AAGGGAGGCGTTAACCAGGCGAATGCCGGTAATCCAAAAGGGGAAAACCAGAACACATGCCGCAACTGAATCCCCGCCTGCGCCAACCGCCCTAACCCCAAccc                                     >  2:826075/1‑139 (MQ=255)                                                                                              tgGATCCGGGGTGGAAAACGATTCAATAATCCCGGCAAAGGGAGCCGTTAGCCAGAAGAGGGCCGTGAATCCAAGAGCGGAAAACCACAACACATGCCTCAACTGAATCTCTGCTTGCGCTAGCTGCCCTACCCCCAAct                                      >  2:728874/1‑139 (MQ=255)                                                                                               ggATCCTGGGTGTAAAACGATGCCATAACCCCGGCAAAGGGAGTCGTTAGCCAGGCGATGGCCGTTAATCCAAGAGTGGAAAGCCAGAACACATGCCGCAACTGAATCTCTGCTTGCGCTATCTGCCCTACCCCCAACCt                                     <  1:114586/140‑1 (MQ=255)                                                                                               ggATCCTGGGTGTAAAACGATGCCATAACCCCGGCAAAGGGAGTCGTTAGCCAGGCGATGGCCGTTAATCCAAGAGTGGAAAGCCAGAACACATGCCGCAACTGAATCTCTGCTTGCGCTATCTGCCCTACCCCCAACCt                                     >  1:294988/1‑140 (MQ=255)                                                                                               ggATCCTGGGTGTAAAACGATGCCATAACCCCGGCAAAGGGAGTCGTTAGCCAGGCGATGGCCGTTAATCCAAGAGTGGAAAGCCAGAACACATGCCGCAACTGAATCTCTGCTTGCGCTATCTGCCCTACCCCCAACCt                                     <  2:525905/140‑1 (MQ=255)                                                                                                        gtgtAAAACGATGCCATAACCCCGGCAAAGGGAGTCGTTAGCCAGGCGATGGCCGTTAATCCAAGAGTGGAAAGCCAGAACACATGCCGCAACTGAATCTCTGCTTGCGCTATCTGCCCTACCCCCAACCTTCGGCCTGt                            >  1:347057/1‑140 (MQ=255)                                                                                                           tAAAACGATGCCATAACCCCGGCAAAGGGAGTCGTTAGCCAGGCGATGGCCGTTAATCCAAGAGTGGAAAGCCAGAACACATGCCGCAACTGAATCTCTGCTTGCGCTATCTGCCCTACCCCCAACCTTCGGCCTGTAAt                         >  2:376869/1‑140 (MQ=255)                                                                                                             aaaCGATGCCATAACCCCGGCAAAGGGAGTCGTTAGCCAGGCGATGGCCGTTAATCCAAGAGTGGAAAGCCAGAACACATGCCGCAACTGAATCTCTGCTTGCGCTATCTGCCCTACCCCCAACCTTCGGc                                >  1:712515/1‑131 (MQ=255)                                                                                                             aaaCGATGCCATAACCCCGGCAAAGGGAGTCGTTAGCCAGGCGATGGCCGTTAATCCAAGAGTGGAAAGCCAGAACACATGCCGCAACTGAATCTCTGCTTGCGCTATCTGCCCTACCCCCAACCTTCGGc                                <  2:712515/131‑1 (MQ=255)                                                                                                             aaaCGATGCCATAACCCCGGCAAAGGGAGTCGTTAGCCAGGCGATGGCCGTTAATCCAAGAGTGGAAAGCCAGAACACATGCCGCAACTGAATCTCTGCTTGCGCTATCTGCCCTACCCCCAACCTTCGGCCTGTaa                          >  1:55170/1‑137 (MQ=255)                                                                                                             aaaCGATGCCATAACCCCGGCAAAGGGAGTCGTTAGCCAGGCGATGGCCGTTAATCCAAGAGTGGAAAGCCAGAACACATGCCGCAACTGAATCTCTGCTTGCGCTATCTGCCCTACCCCCAACCTTCGGCCTGTaa                          <  2:55170/137‑1 (MQ=255)                                                                                                             aaaCGATGCCATAACCCCGGCAAAGGGAGTCGTTAGCCAGGCGATGGCCGTTAATCCAAGAGTGGAAAGCCAGAACACATGCCGCAACTGAATCTCTGCTTGCGCTATCTGCCCTACCCCCAACCTTCGGCCTGTAATGa                       >  2:135914/1‑140 (MQ=255)                                                                                                                 gATGCCATAACCCCGGCAAAGGGAGTCGTTAGCCAGGCGATGGCCGTTAATCCAAGAGTGGAAAGCCAGAACACATGCCGCAACTGAATCTCTGCTTGCGCTATCTGCCCTACCCCCAACCTTCGGCCTGTAATGATCGt                   >  2:128184/1‑140 (MQ=255)                                                                                                                   tGCCATAACCCCGGCAAAGGGAGTCGTTAGCCAGGCGATGGCCGTTAATCCAAGAGTGGAAAGCCAGAACACATGCCGCAACTGAATCTCTGCTTGCGCTATCTGCCCTACCCCCAACCTTCGGCCTGTAATGATCGTAg                 <  1:915244/140‑1 (MQ=255)                                                                                                                   tGCCATAACCCCGGCAAAGGGAGTCGTTAGCCAGGCGATGGCCGTTAATCCAAGAGTGGAAAGCCAGAACACATGCCGCAACTGAATCTCTGCTTGCGCTATCTGCCCTACCCCCAACCTTCGGCCTGTAATGATCGTAg                 >  1:772730/1‑140 (MQ=255)                                                                                                                           ccccGGCAAATGGACTCGTTAGCCAGGCGAAGGCCGTTAATCCAAGAGTGGAAAGCCAGAACACATGCCGCAACTGAATCTCTGCTTGCGCTATCTGCCCTACCCCCAACCTTCGGCCTGTAATGATCGTAGAAGCagag         <  2:597678/140‑1 (MQ=255)                                                                                                                                gCAAAGGGAGTCGTTAGCCAGGCGATGGCCGTTAATCCAAGAGTGGAAAGCCAGAACACATGCCGCAACTGAATCTCTGCTTGCGCTATCTGCCCTACCCCCAACCTTCGGCCTGTAATGATCGTAGAAGCAGAGCCGAg    >  1:299351/1‑140 (MQ=255)                                                                                                                                  aaaGGGAGTCGTTAGCCAGGCGATGGCCGTTAATCCAAGAGTGGAAAGCCAGAACACATGCCGCAACTGAATCTCTGCTTGCGCTATCTGCCCTACCCCCAACCTTCGGCCTGTAATGATCGTAGAAGCAGAGCCGAgcg  <  2:294988/140‑1 (MQ=255)                                                                                                                                  aaaGGGAGTCGTTAGCCAGGCGATGGCCGTTAATCCAAGAGTGGAAAGCCAGAACACATGCCGCAACTGAATCTCTGCTTGCGCTATCTGCCCTACCCCCAACCTTCGGCCTGTAATGATCGTAGAAGCAGAGCCGAgcg  <  1:826075/140‑1 (MQ=255)                                                                                                                                          |                                                                                                                                    TTTAAATCCAGCGGGTAGCACCCATGAGGCGGACCAAATAGGCATAAATAAAGCATTTAGCCAAATCAGAATCACAACGACATGTTTAACCTGTGGATCCTGGGTGTAAAACGATGCCATAACCCCGGCAAAGGGAGCCGTTAGCCAGGCGATGGCCGTTAATCCAAGAGTGGAAAGCCAGAACACATGCCGCAACTGAATCTCTGCTTGCGCTATCTGCCCTACCCCCAACCTTCGGCCTGTAATGATCGTAGAAGCAGAGCCGAGCG  >  NC_000913/2058452‑2058720

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑