Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,094,819	G→A	L95L (TTG→TTA)	hisH →	imidazole glycerol phosphate synthase subunit HisH

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,094,819	0	G	A	100.0%	125.1 / NA	37	L95L (TTG→TTA)	hisH	imidazole glycerol phosphate synthase subunit HisH
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (25/12);  total (25/12)

CGCAAGCGGCGATGGATCAGGTACGTGAGCGCGAGCTGTTTGATCTCATCAAAGCCTGTACCCAACCGGTGCTGGGCATCTGCTTAGGGATGCAACTGCTGGGGCGGCGCAGCGAAGAGAGCAACGGCGTCGACTTGCTGGGCATCATCGACGAAGACGTGCCGAAAATGACCGACTTTGGTCTGCCACTGCCACATATGGGCTGGAACCGCGTTTACCCGCAGGCAGGCAACCGCCTGTTTCAGGGGATTGAAGACGGCGCGTACTTT  >  NC_000913/2094683‑2094951                                                                                                                                         |                                                                                                                                     cgcAAGCGGCGATGGATCAGGTACGTGAGCGCGAGCTGTTTGATCTCATCAAAGCCTGTACCCAACCGGTGCTGGGCATCTGCTTAGGGATGCAACTGCTGGGGCGGCGCAGCGAAGAGAGCAACGGCGTCGACTTACTg                                                                                                                                   >  1:891807/1‑140 (MQ=255)                aTCAGGTACGTGAGCGCGAGCTGTTTGATCTCATCAAAGCCTGTACCCAACCGGTGCTGGGCATCTGCTTAGGGATGCAACTGCTGGGGCGGCGCAGCGAAGAGAGCAACGGCGTCGACTTACTGGGCATCATCGACGaa                                                                                                                    >  2:851112/1‑140 (MQ=255)                        cGTGAGCGCGAGCTGTTTGATCTCATCAAAGCCTGTACCCAACCGGTGCTGGGCATCTGCTTAGGGATGCAACTGCTGGGGCGGCGCAGCGAAGAGAGCAACGGCGTCGACTTACTGGGCATCATCGACGAAGACGTGcc                                                                                                            >  2:280749/1‑140 (MQ=255)                          tGAGCGCGAGCTGCTTGATCTCATCAAAGCCTGTACCCAACCGGTGCTGGGCATCTGCTTAGGGATGCAACTGCTGGGGCGGCGCAGCGAAGAGAGCAACGGCGTCGACTTACTGGGCATCATCGACGAAGACGTGCCGa                                                                                                          >  2:386871/1‑140 (MQ=255)                           gAGCGCGAGCTGTTTGATCTCATCAAAGCCTGTACCCAACCGGTGCTGGGCATCTGCTTAGGGATGCAACTGCTGGGGCGGCGCAGCGAAGAGAGCAACGGCGTCGACTTACTGGGCATCATCGACGAAGACGTGCCGaa                                                                                                         >  2:233786/1‑140 (MQ=255)                              cgcgAGCTGTTTGATCTCATCAAAGCCTGTACCCAACCGGTGCTGGGCATCTGCTTAGGGATGCAACTGCTGGGGCGGCGCAGCGAAGAGAGCAACGGCGTCGACTTACTGGGCATCATCGACGAAGACGTGCCGAAAAt                                                                                                      <  1:851112/140‑1 (MQ=255)                              cgcgAGCTGTTTGATCTCATCAAAGCCTGTACCCAACCGGTGCTGGGCATCTGCTTAGGGATGCAACTGCTGGGGCGGCGCAGCGAAGAGAGCAACGGCGTCGACTTACTGGGCATCATCGACGAAGACGTGCCGAAAAt                                                                                                      >  1:642283/1‑140 (MQ=255)                              cgcgAGCTGTTTGATCTCATCAAAGCCTGTACCCAACCGGTGCTGGGCATCTGCTTAGGGATGCAACTGCTGGGGCGGCGCAGCGAAGAGAGCAACGGCGTCGACTTACTGGGCATCATCGACGAAGACGTGCCGAAAAt                                                                                                      >  1:31709/1‑140 (MQ=255)                               gcgAGCTGTTTGATCTCATCAAAGCCTGTACCCAACCGGTGCTGGGCATCTGCTTAGGGATGCAACTGCTGGGGCGGCGCAGCGAAGAGAGCAACGGCGTCGACTTACTGGGCATCATCGACGAAGACGTGCCGAAAATg                                                                                                     >  1:438408/1‑140 (MQ=255)                               gcgAGCTGTTTGATCTCATCAAAGCCTGTACCCAACCGGTGCTGGGCATCTGCTTAGGGATGCAACTGCTGGGGCGGCGCAGCGAAGAGAGCAACGGCGTCGACTTACTGGGCATCATCGACGAAGACGTGCCGAAAATg                                                                                                     <  2:642283/140‑1 (MQ=255)                                cgAGCTGTTTGATCTCATCAAAGCCTGTACCCAACCGGTGCTGGGCATCTGCTTAGGGATGCAACTGCTGGGGCGGCGCAGCGAAGAGAGCAACGGCGTCGACTTACTGGGCATCATCGACGAAGACGTGCCGAAAATg                                                                                                     <  1:346606/139‑1 (MQ=255)                                cgAGCTGTTTGATCTCATCAAAGCCTGTACCCAACCGGTGCTGGGCATCTGCTTAGGGATGCAACTGCTGGGGCGGCGCAGCGAAGAGAGCAACGGCGTCGACTTACTGGGCATCATCGACGAAGACGTGCCGAAAATg                                                                                                     >  2:346606/1‑139 (MQ=255)                                 gAGCTGTTTGATCTCATCAAAGCCTGTACCCAACCGGTGCTGGGCATCTGCTTAGGGATGCAACTGCTGGGGCGGCGCAGCGAAGAGAGCAACGGCGTCGACTTACTGGGCATCATCGACGAAGACGTGCCGAAAATGAc                                                                                                   <  2:891807/140‑1 (MQ=255)                                                 tcaAAGCCTGTACCCAACCGGTGCTGGGCATCTGCTTAGGGATGCAACTGCTGGGGCGGCGCAGCGAAGAGAGCAACGGCGTCGACTTACTGGGCATCATCGACGAAGACGTGCCGAAAATGACCGACTTTGGTctgcca                                                                                   <  1:280749/140‑1 (MQ=255)                                                 tcaAAGCCTGTACCCAACCGGTGCTGGGCATCTGCTTAGGGATGCAACTGCTGGGGCGGCGCAGCGAAGAGAGCAACGGCGTCGACTTACTGGGCATCATCGACGAAGACGTGCCGAAAATGACCGACTTTGGTctgcca                                                                                   <  1:233786/140‑1 (MQ=255)                                                          gTACCCAACCGGTGCTGGGCATCTGCTTAGGGATGCAACTGCTGGGGCGGCGCAGCGAAGAGAGCAACGGCGTCGACTTACTGGGCATCATCGACGAAGACGTGCCGAAAATGACCGACTTTGGTCTGCCACTGCCACat                                                                          >  1:212011/1‑140 (MQ=255)                                                                aaCCGGTGCTGGGCATCTGCTTAGGGATGCAACTGCTGGGGCGGCGCAGCGAAGAGAGCAACGGCGTCGACTTACTGGGCATCATCGACGAAGACGTGCCGAAAATGACCGACTTTGGTctgccactgc                                                                               >  1:528665/1‑129 (MQ=255)                                                                aaCCGGTGCTGGGCATCTGCTTAGGGATGCAACTGCTGGGGCGGCGCAGCGAAGAGAGCAACGGCGTCGACTTACTGGGCATCATCGACGAAGACGTGCCGAAAATGACCGACTTTGGTctgccactgc                                                                               <  2:528665/129‑1 (MQ=255)                                                                aaCCGGTGCTGGGCATCTGCTTAGGGATGCAACTGCTGGGGCGGCGCAGCGAAGAGAGCAACGGCGTCGACTTACTGGGCATCATCGACGAAGACGTGCCGAAAATGACCGACTTTGGTCTGCCACTGCCACATATGGGc                                                                    <  2:326247/140‑1 (MQ=255)                                                                 aCCGGTGCTGGGCATCTGCTTAGGGATGCAACTGCTGGGGCGGCGCAGCGAAGAGAGCAACGGCGTCGACTTACTGGGCATCATCGACGAAGACGTGCCGAAAATGACCGACTTTGGTCTGCCACTGCCACATATGGGCt                                                                   >  1:519633/1‑140 (MQ=255)                                                                 aCCGGTGCTGGGCATCTGCTTAGGGATGCAACTGCTGGGGCGGCGCAGCGAAGAGAGCAACGGCGTCGACTTACTGGGCATCATCGACGAAGACGTGCCGAAAATGACCGACTTTGGTCTGCCACTGCCACATATGGGCt                                                                   >  2:62986/1‑140 (MQ=255)                                                                 aCCGGTGCTGGGCATCTGCTTAGGGATGCAACTGCTGGGGCGGCGCAGCGAAGAGAGCAACGGCGTCGACTTACTGGGCATCATCGACGAAGACGTGCCGAAAATGACCGACTTTGGTCTGCCACTGCCACATATGGGCt                                                                   >  1:116533/1‑140 (MQ=255)                                                                     gTGCTGGGCATCTGCTTAGGGATGCAACTGCTGGGGCGGCGCAGCGAAGAGAGCAACGGCGTCGACTTACTGGGCATCATCGACGAAGACGTGCCGAAAATGACCGACTTTGGTCTGCCACTGCCACATATGGGCTGGaa                                                               >  2:418420/1‑140 (MQ=255)                                                                           ggCATCTGCTTAGGGATGCAACTGCTGGGGCGGCGCAGCGAAGAGAGCAACGGCGTCGACTTACTGGGCATCATCGACGAAGACGTGCCGAAAATGACCGACTTTGGTCTGCCACTGCCACATATGGGCTGGAACCGCGt                                                         <  2:212011/140‑1 (MQ=255)                                                                                cTGCTTAGGGATGCAACTGCTGGGGCGGCGCAGCGAAGAGAGCAACGGCGTCGACTTACTGGGCATCATCGACGAAGACGTGCCGAAAATGACCGGCTTTGGTCTGCCACTGCCACATATGGGCTGGAACCGCGTTTAcc                                                    <  2:519633/140‑1 (MQ=255)                                                                                       gggATGCAACTGCTGGGGCGGCGCAGCGAAGAGAGCAACGGCGTCGACTTACTGGGCATCATCGACGAAGACGTGCCGAAAATGACCGACTTTGGTCTGCCACTGCCACATATGGGCTGGAACCGCGTTTACCCgcaggc                                             >  1:15055/1‑140 (MQ=255)                                                                                           tGCAACTGCTGGGGCGGCGCAGCGAAGAGAGCAACGGCGTCGACTTACTGGGCATCATCGACGAAGACGTGCCGAAAATGACCGACTTTGGTCTGCCACTGCCACATATGGGCTGGAACCGCGTTTACCCgcaggc                                             >  2:452614/1‑136 (MQ=255)                                                                                           tGCAACTGCTGGGGCGGCGCAGCGAAGAGAGCAACGGCGTCGACTTACTGGGCATCATCGACGAAGACGTGCCGAAAATGACCGACTTTGGTCTGCCACTGCCACATATGGGCTGGAACCGCGTTTACCCgcaggc                                             <  1:452614/136‑1 (MQ=255)                                                                                           tGCAACTGCTGGGGCGGCGCAGCGAAGAGAGCAACGGCGTCGACTTACTGGGCATCATCGACGAAGACGTGCCGAAAATGACCGACTTTGGTCTGCCACTGCCACATATGGGCTGGAACCGCGTTTACCCGCaggcaggc                                         >  1:135061/1‑140 (MQ=255)                                                                                           tGCAACTGCTGGGGCGGCGCAGCGAAGAGAGCAACGGCGTCGACTTACTGGGCATCATCGACGAAGACGTGCCGAAAATGACCGACTTTGGTCTGCCACTGCCACATATGGGCTGGAACCGCGTTTACCCGCaggcaggc                                         >  2:402483/1‑140 (MQ=255)                                                                                           tGCAACTGCTGGGGCGGCGCAGCGAAGAGAGCAACGGCGTCGACTTACTGGGCATCATCGACGAAGACGTGCCGAAAATGACCGACTTTGGTCCGCCACTGCCACATATGGGCTGGAACCGCGTTTACCCGCaggcaggc                                         >  1:465141/1‑140 (MQ=255)                                                                                             cAACTGCGGGGGCGGCGCAGCGAAGAGAGCAACGGCGTCGACTTACGGGGCATCATCGACGAAGACGTGCCGAAAATGCCCGACTTTGGTCTGCCACTGCCACATATGGGCTGGAACCGCGTTTCCCCGCAGGCAGGCaa                                       <  2:845370/140‑1 (MQ=255)                                                                                                  gctgGGGCGGCGCAGCGAAGAGAGCAACGGCGTCGACTTACTGGGCATCATCGACGAAGACGTGCCGAAAATGACCGACTTTGGTCTGCCCCTGCCACATATGGGCTGGAACCGCGTTTACCCGCAGGCAGGCAACCGcc                                  <  2:31709/140‑1 (MQ=255)                                                                                                  gctgGGGCGGCGCAGCGAAGAGAGCAACGGCGTCGACTTACTGGGCATCATCGACGAAGACGTGCCGAAAATGACCGACTTTGGTCTGCCACTGCCACATATGGGCTGGAACCGCGTTTCCCCGCAGGCAGGCAACCGcc                                  <  1:402483/140‑1 (MQ=255)                                                                                                  gctgGGGCGGCGCAGCGAAGAGAGCAACGGCGTCGACTTACTGGGCATCATCGACGAAGACGTGCCGAAAATGACCGACTTTGGTCTGCCACTGCCACATATGGGCTGGAACCGCGTTTACCCGCAGGCAGGCAACCGcc                                  >  2:941645/1‑140 (MQ=255)                                                                                                            cgcAGCGAAGAGAGCAACGGCGTCGACTTACTGGGCATCATCGACGAAGACGTGCCGAAAATGACCGACTTTGGTCTGCCACTGCCACATATGGGCTGGAACCGCGTTTACCCGCAGGCAGGCAACCGCCTGTTTCAggg                        >  2:305949/1‑140 (MQ=255)                                                                                                                            cgggCGTCGATTTAGGGGGCATCATCGACGAAGACGGGCGAAAAATGCCCGTTTTTGGCCCGCCACTGCCACATATGGGCTGGAACCGCGTTTCCCCGCAGGCAGGCAACCGCCTGTTTCAGGGGATTGAAGACGGcgcg        <  2:465141/138‑1 (MQ=255)                                                                                                                            aCGGCGTCGACTTACTGGGCATCATCGACGAAGACGTGCCGAAAATGACCGACTTTGGTCTGCCACTGCCACATATGGGCTGGAACCGCGTTTACCCGCAGGCAGGCAACCGCCTGTTTCAGGGGATTGAAGACGGcgcg        <  2:135061/140‑1 (MQ=255)                                                                                                                                cGTCGACTTACTGGGCATCATCGACGAAGACGTGCCGAAAATGACCGACTTTGGTCTGCCACTGCCACATATGGGCTGGAACCGCGTTTACCCGCAGGCAGGCAACCGCCTGTTTCAGGGGATTGAAGACGGCGCGtact    >  2:368751/1‑140 (MQ=255)                                                                                                                                cGTCGACTTACTGGGCATCATCGACGAAGACGTGCCGAAAATGACCGACTTTGGTCTGCCACTGCCACATATGGGCTGGAACCGCGTTTACCCGCAGGCAGGCAACCGCCTGTTTCAGGGGATTGAAGACGGCGCGtact    >  1:479220/1‑140 (MQ=255)                                                                                                                                  tCGACTTACTGGGCATCATCGACGAAGACGTGCCGAAAATGACCGACTTTGGTCTGCCACTGCCACATATGGGCTGGAACCGCGTTTACCCGCAGGCAGGCAACCGCCTGTTTCAGGGGATTGAAGACGGCGCGtacttt  >  1:152590/1‑140 (MQ=255)                                                                                                                                         |                                                                                                                                     CGCAAGCGGCGATGGATCAGGTACGTGAGCGCGAGCTGTTTGATCTCATCAAAGCCTGTACCCAACCGGTGCTGGGCATCTGCTTAGGGATGCAACTGCTGGGGCGGCGCAGCGAAGAGAGCAACGGCGTCGACTTGCTGGGCATCATCGACGAAGACGTGCCGAAAATGACCGACTTTGGTCTGCCACTGCCACATATGGGCTGGAACCGCGTTTACCCGCAGGCAGGCAACCGCCTGTTTCAGGGGATTGAAGACGGCGCGTACTTT  >  NC_000913/2094683‑2094951

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑