Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,166,814	T→C	F383L (TTC→CTC)	yegQ →	putative peptidase YegQ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,166,814	0	T	C	100.0%	158.7 / NA	45	F383L (TTC→CTC)	yegQ	putative peptidase YegQ
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (19/26);  total (19/26)

GTTTCCTGCGTCGTCATACTCACGACGATTATCAGAACTACGAATACGGTTATTCAGTTTCTGACCGCCAGCAGTTTGTTGGTGAGTTTACCGGTGAGCGCAAGGGGGACCTCGCGGCGGTAGCGGTGAAAAATAAATTCTCCGTTGGCGACAGCCTTGAGCTGATGACGCCGCAAGGCAACATTAATTTTACCCTTGAGCACATGGAAAACGCCAAAGGCGAAGCTATGCCGATAGCACCAGGCGATGGTTAT  >  NC_000913/2166677‑2166930                                                                                                                                          |                                                                                                                     gTTTCCTGCGTCGTCATACTCACGACGATTATCAGAACTACGAATACGGTTATTCAGTTTCTGACCGCCAGCAGTTTGTTGGTGAGTTTACCGGTGAGCGCAAGGGGGACCTCGCGGCGGTAGCGGTGAAAAATAAACtc                                                                                                                    >  1:644375/1‑140 (MQ=255)   ttCCTGCGTCGTCATACTCACGACGATTATCAGAACTACGAATACGGTTATTCAGTTTCTGACCGCCAGCAGTTTGTTGGTGAGTTTACCGGTGAGCGCAAGGGGGACCTCGCGGCGGTAGCGGTGAAAAATAAACtctc                                                                                                                  >  1:131807/1‑140 (MQ=255)            cgtcATACTCACGACGATTATCAGAACTACGAATACGGTTATTCAGTTTCTGACCGCCAGCAGTTTGTTGGTGAGTTTACCGGTGAGCGCAAGGGGGACCTCGCGGCGGTAGCGGTGAAAAATAAACTCTCCGTTGGCGa                                                                                                         <  2:908671/140‑1 (MQ=255)                      acgacgATTATCAGAACTACGAATACGGTTATTCAGTTTCTGACCGCCAGCAGTTTGTTGGTGAGTTTACCGGTGAGCGCAAGGGGGACCTCGCGGCGGTAGCGGTGAAAAATAAACTCTCCGTTGGCGACAGCCTTGAg                                                                                               >  1:393881/1‑140 (MQ=255)                      acgacgATTATCAGAACTACGAATACGGTTATTCAGTTTCTGACCGCCAGCAGTTTGTTGGTGAGTTTACCGGTGAGCGCAAGGGGGACCTCGCGGCGGTAGCGGTGAAAAATAAACTCTCCGTTGGCGACAGCCTTGAg                                                                                               >  2:556195/1‑140 (MQ=255)                        gacgaTTATCAGAACTACGAATACGGTTATTCAGTTTCTGACCGCCAGCAGTTTGTTGGTGAGTTTACCGGTGAGCGCAAGGGGGACCTCGCGGCGGTAGCGGTGAAAAATAAACTCTCCGTTGGCGACAGCCTTGAGCt                                                                                             >  2:4193/1‑140 (MQ=255)                        gacgaTTATCAGAACTACGAATACGGTTATTCAGTTTCTGACCGCCAGCAGTTTGTTGGTGAGTTTACCGGTGAGCGCAAGGGGGACCTCGCGGCGGTAGCGGTGAAAAATAAACTCTCCGTTGGCGACAGCCTTGAGCt                                                                                             >  2:43016/1‑140 (MQ=255)                        gacgaTTATCAGAAATACGAATACGGGTATTCAGTTTCTGACCGCCAGCAG‑TTGTTGGTGAGTTTACCGGTGAGCGCAAGGGGGACCTCGCGGCGGGAGCGGGGGAAAAAAAACTCTCCGTTGGGGGCAGCCCTGAGCtg                                                                                            >  2:496509/1‑140 (MQ=255)                           gaTTATCAGAACTACGAATACGGTTATTCAGTTTCTGACCGCCAGCAGTTTGTTGGTGAGTTTACCGGTGAGCGCAAGGGGGACCTCGCGGCGGTAGCGGTGAAAAATAAACTCTCCGTTGGCGACAGCCTTGAGCtgat                                                                                          <  1:469689/140‑1 (MQ=255)                             ttATCAGAACTACGAATACGGTTATTCAGTTTCTGACCGCCAGCAGTTTGTTGGTGAGTTTACCGGTGAGCGCAAGGGGGACCTCGCGGCGGTAGCGGTGAAAAATAAACTCTCCGTTGGCGACAGCCTTGAGCtgatga                                                                                        >  1:517860/1‑140 (MQ=255)                                    aaCTACGAATACGGTTATTCAGTTTCTGACCGCCAGCAGTTTGTTGGTGAGTTTACCGGTGAGCGCAAGGGGGACCTCGCGGCGGTAGCGGTGAAAAATAAACTCTCCGTTGGCGACAGCCTTGAGCTGATGACGCCGCa                                                                                 <  2:393881/140‑1 (MQ=255)                                     tcTACGAATACGGTTATTCAGTTTCTGACCGCCAGCAGTTTGTTGGTGAGTTTACCGGTGAGCGCAAGGGGGACCTCGCGGCGGTAGCGGTGAAAAATAAACTCTCCGTTGGCGACAGCCTTGAGCtgatga                                                                                        >  2:126892/2‑132 (MQ=255)                                     tcTACGAATACGGTTATTCAGTTTCTGACCGCCAGCAGTTTGTTGGTGAGTTTACCGGTGAGCGCAAGGGGGACCTCGCGGCGGTAGCGGTGAAAAATAAACTCTCCGTTGGCGACAGCCTTGAGCtgatga                                                                                        <  1:126892/131‑1 (MQ=255)                                       tACGAATACGGTTATTCAGTTTCTGACCGCCAGCAGTTTGTTGGTGAGTTTACCGGTGAGCGCAAGGGGGACCTCGCGGCGGTAGCGGTGAAAAATAAACTCTCCGTTGGCGACAGCCTTGAGCTGATGACGCCGCAAg                                                                               >  2:488566/1‑139 (MQ=255)                                       tACGAATACGGTTATTCAGTTTCTGACCGCCAGCAGTTTGTTGGTGAGTTTACCGGTGAGCGCAAGGGGGACCTCGCGGCGGTAGCGGTGAAAAATAAACTCTCCGTTGGCGACAGCCTTGAGCTGATGACGCCGCAAg                                                                               <  1:488566/139‑1 (MQ=255)                                              aCGGTTATTCAGTTTCTGATCGCCAGCAGTTTGTTGGTGAGTTTACCGGTGAGCGCAAGGGGGACCTCGCGGCGGTAGCGGTGAAAAATAAACTCTCCGTTGGCGACAGCCTTGAGCTGATGACGCCGCAAGGCAACAtt                                                                       <  1:665411/140‑1 (MQ=255)                                              aCGGTTATTCAGTTTCTGACCGCCAGCAGTTTGTTGGTGAGTTTACCGGTGAGCGCAAGGGGGACCTCGCGGCGGTAGCGGTGAAAAATAAACTCTCCGTTGGCGACAGCCTTGAGCTGATGACGCCGCAAGGCAACAtt                                                                       >  2:109173/1‑140 (MQ=255)                                                  ttATTCAGTTTCTGACCGCCAGCAGTTTGTTGGTGAGTTTACCGGTGAGCGCAAGGGGGACCTCGCGGCAGTAGCGGTGAAAAATAAACTCTCCGTTGGCGACAGCCTTGAGCTGATGACGCCGCAAGGCAACATTAAtt                                                                   >  2:601316/1‑140 (MQ=255)                                                   tATTCAGTTTCTGACCGCCAGCAGTTTGTTGGTGAGTTTACCGGTGAGCGCAAGGGGGACCTCGCGGCGGTAGCGGTGAAAAATAAACTCTCCGTTGGCGACAGCCTTGAGCTGATGACGCCGCAAGGCAACATTAAttt                                                                  <  1:43016/140‑1 (MQ=255)                                                   tATTCAGTTTCTGACCGCCAGCAGTTTGTTGGTGAGTTTACCGGTGAGCGCAAGGGGGACCTCGCGGCGGTAGCGGTGAAAAATAAACTCTCCGTTGGCGACAGCCTTGAGCTGATGACGCCGCAAGGCAACATTAAttt                                                                  <  1:820833/140‑1 (MQ=255)                                                          tttCTGACCGCCAGCAGTTTGTTGGTGAGTTTACCGGTGAGCGCAAGGGGGACCTCGCGGCGGTAGCGGTGAAAAATAAACTCTCCGTTGGCGACAGCCTTGAGCTGATGACGCCGCAAGGCAACATTaa                                                                     <  2:293051/130‑1 (MQ=255)                                                          tttCTGACCGCCAGCAGTTTGTTGGTGAGTTTACCGGTGAGCGCAAGGGGGACCTCGCGGCGGTAGCGGTGAAAAATAAACTCTCCGTTGGCGACAGCCTTGAGCTGATGACGCCGCAAGGCAACATTaa                                                                     >  1:293051/1‑130 (MQ=255)                                                           ttCTGACCGCCAGCAGTTTGTTGGTGAGTTTACCGGTGAGCGCAAGGGGGACCTCGCGGCGGTAGCGGTGAAAAATAAACTCTCCGTTGGCGACAGCCTTGAGCTGATGACGCCGCAAGGCAACATTAATTTTACCCtt                                                           <  1:40091/139‑1 (MQ=255)                                                           ttCTGACCGCCAGCAGTTTGTTGGTGAGTTTACCGGTGAGCGCAAGGGGGACCTCGCGGCGGTAGCGGTGAAAAATAAACTCTCCGTTGGCGACAGCCTTGAGCTGATGACGCCGCAAGGCAACATTAATTTTACCCtt                                                           >  2:40091/1‑139 (MQ=255)                                                            tCTGACCGCCAGCAGTTTGTTGGTGAGTTTACCGGTGAGCGCAAGGGGGACCTCGCGGCGGTAGCGGTGAAAAATAAACTCTCCGTTGGCGACAGCCTTGAGCTGATGACGCCGCAAGGCAACATTAATTTTACCCTTGa                                                         >  1:365951/1‑140 (MQ=255)                                                             cTGACCGCCAGCAGTTTGTTGGTGAGTTTACCGGTGAGCGCAAGGGGGACCTCGCGGCGGTAGCGGTGAAAAATAAACTCTCCGTTGGCGACAGCCTTGAGCTGATGACGCCGCAAGGCAACATTAATTTTACCCTTGAg                                                        <  2:356423/140‑1 (MQ=255)                                                             cTGACCGCCAGCAGTTTGTTGGTGAGTTTACCGGTGAGCGCAAGGGGGACCTCGCGGCGGTAGCGGTGAAAAATAAACTCTCCGTTGGCGACAGCCTTGAGCTGATGACGCCGCAAGGCAACATTAATTTTACCCTTGAg                                                        <  2:885030/140‑1 (MQ=255)                                                                                 ggTGAGTTTACCGGTGAGCGCAAGGGGGACCTCGCGGCGGTAGCGGTGAAAAATAAACTCTCCGTTGGCGACAGCCTTGAGCTGATGACGCCGCAAGGCAACATTAATTTTACCCTTGAGCACATGGAAAACGCCAAAgg                                    <  2:449494/140‑1 (MQ=255)                                                                                 ggTGAGTTTACCGGTGAGCGCAAGGGGGACCTCGCGGCGGTAGCGGTGAAAAATAAACTCTCCGTTGGCGACAGCCTTGAGCTGATGACGCCGCAAGGCAACATTAATTTTACCCTTGAGCACATGGAAAACGCCAAAgg                                    <  2:228513/140‑1 (MQ=255)                                                                                          aCCGGTGAGCGCAAGGGGGACCTCGCGGCGGTAGCGGTGAAAAATAAACTCTCCGTTGGCGACAGCCTTGAGCTGATGACGCCGCAAGGCAACATTAATTTTACCCTTGAGCACATGGAAAACGCCAAAGGCGAAGCTAt                           <  1:4193/140‑1 (MQ=255)                                                                                           ccGGTGAGCGCAAGGGGGACCTCGCGGCGGTAGCGGTGAAAAATAAACTCTCCGTTGGCGACAGCCTTGAGCTGATGACGCCGCAAGGCAACATTAATTTTACCCTTGAGCACATGGAAAACGCCAAAGGCGAAGCTATg                          <  2:963872/140‑1 (MQ=255)                                                                                                 aGCGCAAGGGGGACCTCGCGGCGGTAGCGGTGAAAAATAAACTCTCCGTTGGCGACAGCCTTGAGCTGATGACGCCGCAAGGCAACATTAATTTTACCCTTGAGCACATGGAAAACGCCAAAGGCGAAGCTATGCCGATa                    >  2:258627/1‑140 (MQ=255)                                                                                                 aGCGCAAGGGGGACCTCGCGGCGGTAGCGGTGAAAAATAAACTCTCCGTTGGCGACAGCCTTGAGCTGATGACGCCGCAAGGCAACATTAATTTTACCCTTGAGCACATGGAAAACGCCAAAGGCGAAGCTATGCCGATa                    >  1:867994/1‑140 (MQ=255)                                                                                                        gggggACCTCGCGGCGGTAGCGGTGAAAAATAAACTCTCCGTTGGCGACAGCCTTGAGCTGATGACGCCGCAAGGCAACATTAATTTTACCCTTGAGCACATGGAAAACGCCAAAGGCGAAGCTATGCCGATAGCACCAg             >  2:69333/1‑140 (MQ=255)                                                                                                          gggACCTCGCGGCGGTAGCGGTGAAAAATAAACTCTCCGTTGGCGACAGCTTTGAGCTGACGACGCCGCAAGGCAACATTAATTTTACCCTTGAGCACATGGAAAACGCCAAAGGCGAAGCTATGCCGATAGCACCAGGc           <  2:867994/140‑1 (MQ=255)                                                                                                          gggACCTCGCGGCGGTAGCGGTGAAAAATAAACTCTCCGTTGGCGACAGCCTTGAGCTGATGACGCCGCAAGGCAACATTAATTTTACCCTTGAGCACATGGAAAACGCCAAAGGCGAAGCTATGCCGATAGCACCAGGc           <  2:131807/140‑1 (MQ=255)                                                                                                          gggACCTCGCGGCGGTAGCGGTGAAAAATAAACTCTCCGTTGGCGACAGCCTTGAGCTGATGACGCCGCAAGGCAACATTAATTTTACCCTTGAGCACATGGAAAACGCCAAAGGCGAAGCTATGCCGATAGCACCAGGc           <  1:556195/140‑1 (MQ=255)                                                                                                          gggACCTCGCGGCGGTAGCGGTGAAAAATAAACTCTCCGTTGGCGACAGCCTTGAGCTGATGACGCCGCAAGGCAACATTAATTTTACCCTTGAGCACATGGAAAACGCCAAAGGCGAAGCTATGCCGATAGCACCAGGc           <  2:644375/140‑1 (MQ=255)                                                                                                            gACCTCGCGGCGGTAGCGGTGAAAAATAAACTCTCCGTTGGCGACAGCCTTGAGCTGATGACGCCGCAAGGCAACATTAATTTTACCCTTGAGCACATGGAAAACGCCAAAGGCGAAGCTATGCCGATAGCACCAGGCGa         <  2:365951/140‑1 (MQ=255)                                                                                                            gACCTCGCGGCGGTAGCGGTGAAAAATAAACTCTCCGTTGGCGACAGCCTTGAGCTGATGACGCCGCAAGGCAACATTAATTTTACCCTTGAGCACATGGAAAACGCCAAAGGCGAAGCTATGCCGATAGCACCAGGCGa         <  1:69333/140‑1 (MQ=255)                                                                                                            gACCTCGCGGCGGTAGCGGTGAAAAATAAACTCTCCGTTGGCGACAGCCTTGAGCTGATGACGCCGCAAGGCAACATTAATTTTACCCTTGAGCACATGGAAAACGCCAAAGGCGAAGCTATGCCGATAGCACCAGGCGa         <  2:725965/140‑1 (MQ=255)                                                                                                            gACCTCGCGGCGGTAGCGGTGAAAAATAAACTCTCCGTTGGCGACAGCCTTGAGCTGATGACGCCGCAAGGCAACATTAATTTTACCCTTGAGCACATGGAAAACGCCAAAGGCGAAGCTATGCCGATAGCACCAGGCGa         <  1:307090/140‑1 (MQ=255)                                                                                                             aCCTCGCGGCGGTAGCGGTGAAAAATAAACTCTCCGTTGGCGACAGCCTTGAGCTGATGACGCCGCAAGGCAACATTAATTTTACCCTTGAGCACATGGAAAACGCCAAAGGCGAAGCTATGCCGATAGCACCAGGCGAt        >  1:684817/1‑140 (MQ=255)                                                                                                               cTCGCGGCGGTAGCGGTGAAAAATAAACTCTCCGTTGGCGACAGCCTTGAGCTGATGACGCCGCAAGGCAACATTAATTTTACCCTTGAGCACATGGAAAACGCCAAAGGCGAAGCTATGCCGATAGCACCAGGCGATgg      <  2:684817/140‑1 (MQ=255)                                                                                                               cTCGCGGCGGTAGCGGTGAAAAATAAACTCTCCGTTGGCGACAGCCTTGAGCTGATGACGCCGCAAGGCAACATTAATTTTACCCTTGAGCACATGGAAAACGCCAAAGGCGAAGCTATGCCGATAGCACCAGGCGATgg      <  1:258627/140‑1 (MQ=255)                                                                                                                   cggcggTAGCGGTGAAAAATAAACTCTCCGTTGGCGACAGCCTTGAGCTGATGACGCCGCAAGGCAACATTAATTTTACCCTTGAGCACATGGAAAACGCCAAAGGCGAAGCTATGCCGATAGCACCAGGCGATGGTtat  >  1:281416/1‑140 (MQ=255)                                                                                                                                          |                                                                                                                     GTTTCCTGCGTCGTCATACTCACGACGATTATCAGAACTACGAATACGGTTATTCAGTTTCTGACCGCCAGCAGTTTGTTGGTGAGTTTACCGGTGAGCGCAAGGGGGACCTCGCGGCGGTAGCGGTGAAAAATAAATTCTCCGTTGGCGACAGCCTTGAGCTGATGACGCCGCAAGGCAACATTAATTTTACCCTTGAGCACATGGAAAACGCCAAAGGCGAAGCTATGCCGATAGCACCAGGCGATGGTTAT  >  NC_000913/2166677‑2166930

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑