Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,167,896	C→T	intergenic (‑76/+93)	yegZ ← / ← yegR	phage protein D/uncharacterized protein YegR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,167,896	0	C	T	100.0%	168.3 / NA	48	intergenic (‑76/+93)	yegZ/yegR	phage protein D/uncharacterized protein YegR
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (25/23);  total (25/23)

TAAATATCTGCCCGACCGGTAGCCAGGCTGATGGAGAACTCTGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGTAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCTTTCACTTATCGGCATCGTGCTCCCACAGGTTCCGGCTACGCACAGCCAGAACGCGCATATTTGACGCTTACCAAAAAATATTCTCACTCTCCACATTTGAATGTCAGACGAGCGACACCATGTAA  >  NC_000913/2167768‑2168030                                                                                                                                 |                                                                                                                                       tAAATATCTGCCCGACCGGTAGCCAGGCTGATGGAGAACTCTGCTAC‑CGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGTAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGTACGATGCCTTTc                                                                                                                            >  1:455935/1‑140 (MQ=255)          gCCCGACCGGTAGCCAGGCTGATGGAGAACTCTGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGTAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGTACGATGCCTTTCACTTATCg                                                                                                                    <  1:476065/140‑1 (MQ=255)                        cAGGCTGATGGAGAACTCTGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGTAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGTACGATGCCTTTCACTTATCGGCATCGTGCTCCca                                                                                                      <  1:363724/140‑1 (MQ=255)                        cAGGCTGATGGAGAACTCTGCTACACAGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGTAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGTACGATGCCTTTCACTTATCGGCATCGTGCTCCca                                                                                                      <  2:283470/140‑1 (MQ=255)                                  gagaACTCTGCTACACGGGCTTA‑TTTTTTATGCATAAGCCCTATCTCTGGTAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGTACGATGCCTTTCACTTATCGGCATCGTGCTCCCACAGGTTCCGGc                                                                                           <  1:878917/140‑1 (MQ=255)                                   agaACTCTGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGTAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGTACGATGCCTTTCACTTATCGGCATCGTGCTCCCACAGGTTCCGGc                                                                                           <  1:154410/140‑1 (MQ=255)                                      aCTCTGCTAC‑CGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGTAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGTACGATGCCTTTCACTTATCGGCATCGTGCTCCCACAGGTTCCGGCTACg                                                                                       <  2:455935/140‑1 (MQ=255)                                       ctctGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGTAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGTACGATGCCTTTCACTTATCGGCATCGTGCTCCCACAGGTTCCGGCTACg                                                                                       <  2:231488/140‑1 (MQ=255)                                         ctGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGTAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGTACGATGCCTTTCCCTTATCGGCATCGTGCTCCCACAGGTTCCGGCTACGca                                                                                     >  1:357209/1‑140 (MQ=255)                                         ctGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGTAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGTACGATGCCTTTCACTTATCGGCATCGTGCTCCCACAGGTTCCGGCTACGca                                                                                     >  2:273852/1‑140 (MQ=255)                                                    gCTTATTTTTTTATGCATAAGCCCTATCTCTGGTAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGTACGATGCCTTTCACTTATCGGCATCGTGCTCCCACAGGTTCCGGCTACGCACAGCCAGAAcg                                                                          >  1:818020/1‑140 (MQ=255)                                                    gCTTATTTTTTTATGCATAAGCCCTATCTCTGGTAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGTACGATGCCTTTCACTTATCGGCATCGTGCTCCCACAGGTTCCGGCTACGCACAGCCAGAAcg                                                                          <  1:227712/140‑1 (MQ=255)                                                      ttATTTTTTTATGCATAAGCCCTATCTCTGGTAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGTACGATGCCTTTCACTTATCGGCATCGTGCTCCCACAGGTTCCGGCTACg                                                                                       <  2:956116/125‑1 (MQ=255)                                                      ttATTTTTTTATGCATAAGCCCTATCTCTGGTAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGTACGATGCCTTTCACTTATCGGCATCGTGCTCCCACAGGTTCCGGCTACg                                                                                       >  1:956116/1‑125 (MQ=255)                                                       tATTTTTTTATGCATAAGCCCTATCTCTGGTAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGTACGATGCCTTTCACTTATCGGCATCGTGCTCCCACAGGTTCCGGCTACGCACAGCCAGAACgcgc                                                                       <  2:357209/140‑1 (MQ=255)                                                            ttttATGCATAAGCCCTATCTCTGGTAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGTACGATGCCTTTCACTTATCGGCATCGTGCTCCCACAGGTTCCGGCTACGCACAGCCAGa                                                                             >  2:249691/1‑129 (MQ=255)                                                            ttttATGCATAAGCCCTATCTCTGGTAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGTACGATGCCTTTCACTTATCGGCATCGTGCTCCCACAGGTTCCGGCTACGCACAGCCAGa                                                                             <  1:249691/129‑1 (MQ=255)                                                                   cATAAGCCCTATCTCTGGTAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGTACGATGCCTTTCACTTATCGGCATCGTGCTCCCACAGGTTCCGGCTACGCACAGCCAGAACGCGCATATTTGACGCt                                                           <  2:818020/140‑1 (MQ=255)                                                                      aaGCCCTATCTCTGGTAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGTACGATGCCTTTCACTTATCGGCATCGTGCTCCCACAGGTTCCGGCTACGCACAGCCAGAACGCGCATATTTGACGCTTAc                                                        >  1:501582/1‑140 (MQ=255)                                                                              tctcTGGTAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGTACGATGCCTTTCACTTATCGGCATCGTGCTCCCACAGGTTCCGGCTACGCACAGCCAGAACGCGCATATTTGACGCTTAcc                                                       >  2:773938/1‑133 (MQ=255)                                                                              tctcTGGTAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGTACGATGCCTTTCACTTATCGGCATCGTGCTCCCACAGGTTCCGGCTACGCACAGCCAGAACGCGCATATTTGACGCTTAcc                                                       <  1:773938/133‑1 (MQ=255)                                                                              tctcTGGTAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGTACGATGCCTTTCACTTATCGGCATCGTGCTCCCACAGGTTCCGGCTACGCACAGCCAGAACGCGCATATTTGACGCTTACCAAAAAat                                                >  2:382376/1‑140 (MQ=255)                                                                              tctcTGGTAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGTACGATGCCTTTCACTTATCGGCATCGTGCTCCCACAGGTTCCGGCTACGCACAGCCAGAACGCGCATATTTGACGCTTACCAAAAAat                                                >  2:217997/1‑140 (MQ=255)                                                                                tctGGTAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGTACGATGCCTTTCACTTATCGGCATCGTGCTCCCACAGGTTCCGGCTACGCACAGCCAGAACGCGCATATTTGACGCTTACCAAAAAatat                                              <  2:501582/140‑1 (MQ=255)                                                                                   ggTAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGTACGATGCCTTTCACTTATCGGCATCGTGCTCCCACAGGTTCCGGCTACGCACAGCCAGAACGCGCATATTTGACGCTTACCAAAAAATATtct                                           >  2:965458/1‑140 (MQ=255)                                                                                   ggTAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGTACGATGCCTTTCACTTATCGGCATCGTGCTCCCACAGGTTCCGGCTACGCACAGCCAGAACGCGCATATTTGACGCTTACCAAAAAATATtct                                           >  2:954032/1‑140 (MQ=255)                                                                                     tAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGTACGATGCCTTTCACTTATCGGCATCGTGCTCCCACAGGTTCCGGCTACGCACAGCCAGAACGCGCATATTTGACGCTTACCAAAAAATATTCTCa                                         <  1:954032/140‑1 (MQ=255)                                                                                        ccGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGTACGATGCCTTTCACTTATCGGCATCGTGCTCCCACAGGTTCCGGCTACGCACAGCCAGAACGCGCATATTTGACGCTTACCAAAAAATATTCTCActc                                      >  2:371725/1‑140 (MQ=255)                                                                                        ccGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGTACGATGCCTTTCACTTATCGGCATCGTGCTCCCACAGGTTCCGGCTACGCACAGCCAGAACGCGCATATTTGACGCTTACCAAAAAATATTCTCActc                                      >  1:358499/1‑140 (MQ=255)                                                                                            cTTCCATTGACCACATCGATAGAATCCTCCTTCATAGTACGATGCCTTTCACTTATCGGCATCGTGCTCCCACAGGTTCCGGCTACGCACAGCCAGAACGCGCATATTTGACGCTTACCAAAAAATATTCTCACTCTCca                                  >  2:361768/1‑140 (MQ=255)                                                                                                       cacaTCGATAGAATCCTCCTTCATAGTACGATGCCTTTCACTTATCGGCATCGTGCTCCCACAGGTTCCGGCTACGCACAGCCAGAACGCGCATATTTGACGCTTACCAAAAAATATTCTCACTCTCCACATTTGAATGt                       >  1:938436/1‑140 (MQ=255)                                                                                                         caTCGATAGAATCCTCCTTCATAGTACGATGCCTTTCACTTATCGGCATCGTGCTCCCACAGGTTCCGGCTACGCACAGCCAGAACGCGCATATTTGACGCTTACCAAAAAATATTCTCACTCTCCACATTTGAATGTCa                     >  2:648590/1‑140 (MQ=255)                                                                                                          aTCGATAGAATCCTCCTTCATAGTACGATGCCTTTCACTTATCGGCATCGTGCTCCCACAGGTTCCGGCTACGCACAGCCAGAACGCGCATATTTGACGCTTACCAAAAAATATTCTCACTCTCCACATTTGAATGTCAg                    >  1:892650/1‑140 (MQ=255)                                                                                                            cGATAGAATCCTCCTTCATAGTACGATGCCTTTCACTTATCGGCATCGTGCTCCCACAGGTTCCGGCTACGCACAGCCAGAACGCGCATATTTGACGCTTACCAAAAAATATTCTCACTCTCCACATTTGAATGTCAGAc                  <  2:892650/140‑1 (MQ=255)                                                                                                            cGATAGAATCCTCCTTCATAGTACGATGCCTTTCACTTATCGGCATCGTGCTCCCACAGGTTCCGGCTACGCACAGCCAGAACGCGCATATTTGACGCTTACCAAAAAATATTCTCACTCTCCACATTTGAATGTCAGAc                  >  1:726635/1‑140 (MQ=255)                                                                                                            cGATAGAATCCTCCTTCATAGTACGATGCCTTTCACTTATCGGCATCGTGCTCCCACAGGTTCCGGCTACGCACAGCCAGAACGCGCATATTTGACGCTTACCAAAAAATATTCTCACTCTCCACATTTGAATGTCAGAc                  <  1:273852/140‑1 (MQ=255)                                                                                                                  aaTCCTCCTTCATAGTACGATGCCTTTCACTTATCGGCATCGTGCTCCCACAGGTTCCGGCTACGCACAGCCAGAACGCGCATATTTGACGCTTACCAAAAAATATTCTCACTCTCCACATTTGAATGTCAGACGAGCGa            <  1:371725/140‑1 (MQ=255)                                                                                                                    tcctccTTCATAGTACGATGCCTTTCACTTATCGGCATCGTGCTCCCACAGGTTCCGGCTACGCACAGCCAGAACGCGCATATTTGACGCTTACCAAAAAATATTCTCACTCTCCACATTTGAATGTCAGACGAGCGaca          <  2:358499/140‑1 (MQ=255)                                                                                                                    tcctccTTCATAGTACGATGCCTTTCACTTATCGGCATCGTGCTCCCACAGGTTCCGGCTACGCACAGCCAGAACGCGCATATTTGACGCTTACCAAAAAATATTCTCACTCTCCACATTTGAATGTCAGACGAGCGaca          <  1:361768/140‑1 (MQ=255)                                                                                                                    tcctccTTCATAGTACGATGCCTTTCACTTATCGGCATCGTGCTCCCACAGGTTCCGGCTACGCACAGCCAGAACGCGCATATTTGACGCTTACCAAAAAATATTCTCACTCTCCACATTTGAATGTCAGACGAGCGaca          >  2:816952/1‑140 (MQ=255)                                                                                                                      ctcctTCATAGTACGATGCCTTTCACTTATCGGCATCGTGCTCCCACAGGTTCCGGCTACGCACAGCCAGAACGCGCATATTTGACGCTTACCAAAAAATATTCTCACTCTCCACATTTGAATGTCAGACGAGCGACAcc        >  2:180410/1‑140 (MQ=255)                                                                                                                      ctcctTCATAGTACGATGCCTTTCACTTATCGGCATCGTGCTCCCACAGGTTCCGGCTACGCACAGCCAGAACGCGCATATTTGACGCTTACCAAAAAATATTCTCACTCTCCACATTTGAATGTCAGACGAGCGACAcc        >  2:811911/1‑140 (MQ=255)                                                                                                                       tcctTCATAGTACGATGCCTTTCACTTATCGGCATCGTGCTCCCACAGGTTCCGGCTACGCACAGCCAGAACGCGCATATTTGACGCTTACCAAAAAATATTCTCACTCTCCACATTTGAATGTCAGACGAGCGACACCa       >  1:392870/1‑140 (MQ=255)                                                                                                                         ctTCATAGTACGATGCCTTTCACTTATCGGCATCGTGCTCCCACAGGTTCCGGCTACGCACAGCCAGAACGCGCATATTTGACGCTTACCAAAAAATATTCTCACTCTCCACATTTGAATGTCAGACGAGCGACACCATg     <  2:392870/140‑1 (MQ=255)                                                                                                                         ctTCATAGTACGATGCCTTTCACTTATCGGCATCGTGCTCCCACAGGTTCCGGCTACGCACAGCCAGAACGCGCATATTTGACGCTTACCAAAAAATATTCTCACTCTCCACATTTGAATGTCAGACGAGCGACACCATg     >  2:129616/1‑140 (MQ=255)                                                                                                                         ctTCATAGTACGATGCCTTTCACTTATCGGCATCGTGCTCCCACAGGTTCCGGCTACGCACAGCCAGAACGCGCATATTTGACGCTTACCAAAAAATATTCTCACTCTCCACATTTGAATGTCAGACGAGCGACACCATg     <  1:129616/140‑1 (MQ=255)                                                                                                                          ttCATAGTACGATGCCTTTCACTTATCGGCATCGTGCTCCCACAGGTTCCGGCTACGCACAGCCAGAACGCGCATATTTGACGCTTACCAAAAAATATTCTCACTCTCCACATTTGAATGTCAGACGAGCGACACCATGt    >  1:11121/1‑140 (MQ=255)                                                                                                                            cATAGTACGATGCCTTTCACTTATCGGCATCGTGCTCCCACAGGTTCCGGCTACGCACAGCCAGAACGCGCATATTTGACGCTTACCAAAAAATATTCTCACTCTCCACATTTGAATGTCAGACGAGCGACACCATGTaa  <  2:726635/140‑1 (MQ=255)                                                                                                                                 |                                                                                                                                       TAAATATCTGCCCGACCGGTAGCCAGGCTGATGGAGAACTCTGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGTAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCTTTCACTTATCGGCATCGTGCTCCCACAGGTTCCGGCTACGCACAGCCAGAACGCGCATATTTGACGCTTACCAAAAAATATTCTCACTCTCCACATTTGAATGTCAGACGAGCGACACCATGTAA  >  NC_000913/2167768‑2168030

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑