Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,279,635	T→C	V42V (GTA→GTG)	bcr ←	multidrug efflux pump Bcr

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,279,635	0	T	C	100.0%	148.6 / NA	42	V42V (GTA→GTG)	bcr	multidrug efflux pump Bcr
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (22/20);  total (22/20)

GCAAACACCAGCGTACCGCCGAGCACCACCGGCTTACGCCCGAAGCTGTCTGCCATCGGCCCGTAGATTAACTGCCCCAACGCAAAGCCCAGAATATAAGTACTGAGGGTCATCTGCGTACTGCCCGCCGGTACGCCAAACTGCGCTGAAATTACCGGTAGCGCGGGCAGATACATATCAATCGACAGCGGCATCAACATGGCCAGCAGGCCAAGGATAAAAACAATAGCAAACGACGAATGCTGTCGGGTGGTCACAACGGG  >  NC_000913/2279504‑2279766                                                                                                                                    |                                                                                                                                    gcAAACACCAGCGTACCGCCGAGCACCACCGGCTTACGCCCGAAGCTGTCTGCCATCGGCCCGTAGATTAACTGCCCCAACGCAAAGCCCAGAATATAAGTACTGAGGGTCATCTGCGTACTGCCCGCCGGCACGCCaaa                                                                                                                             <  1:863945/140‑1 (MQ=255)   aaaCACCAGCGTACCGCCGAGCACCACCGGCTTACGCCCGAAGCTGTCTGCCATCGGCCCGTAGATTAACTGCCCCAACGCAAAGCCCAGAATATAAGTACTGAGGGTCATCTGCGTACTGCCCGCCGGCACGCCAAACt                                                                                                                           <  2:573097/140‑1 (MQ=255)           gCGTACCGCCGAGCACCACCGGCTTACGCCCGAAGCTGTCTGCCATCGGCCCGTAGATTAACTGCCCCAACGCAAAGCCCAGAATATAAGTACTGAGGGTCATCTGCGTACTGCCCGCCGGCACGCCAAACTGCGCTGaa                                                                                                                   >  1:735279/1‑140 (MQ=255)            cGTACCGCCGAGCACCACCGGCTTACGCCCGAAGCTGTCTGCCATCGGCCCGTAGATTAACTGCCCCAACGCAAAGCCCAGAATATAAGTACTGAGGGTCATCTGCGTACTGCCCGCCGGCACGCCAAACTGCGCt                                                                                                                      <  1:38222/136‑1 (MQ=255)            cGTACCGCCGAGCACCACCGGCTTACGCCCGAAGCTGTCTGCCATCGGCCCGTAGATTAACTGCCCCAACGCAAAGCCCAGAATATAAGTACTGAGGGTCATCTGCGTACTGCCCGCCGGCACGCCAAACTGCGCt                                                                                                                      >  2:38222/1‑136 (MQ=255)              tACCGCCGAGCACCACCGGCTTACGCCCGAAGCTGTCTGCCATCGGCCCGTAGATTAACTGCCCCAACGCAAAGCCCAGAATATAAGTACTGAGGGTCATCTGCGTACTGCCCGCCGGCACGCCAAACTgcg                                                                                                                        >  1:277802/1‑132 (MQ=255)              tACCGCCGAGCACCACCGGCTTACGCCCGAAGCTGTCTGCCATCGGCCCGTAGATTAACTGCCCCAACGCAAAGCCCAGAATATAAGTACTGAGGGTCATCTGCGTACTGCCCGCCGGCACGCCAAACTgcg                                                                                                                        <  2:277802/132‑1 (MQ=255)                 cgccgAGCACCACCGGCTTACGCCCGAAGCTGTCTGCCATCGGCCCGTAGATTAACTGCCCCAACGCAAAGCCCAGAATATAAGTACTGAGGGTCATCTGCGTACTGCCCGCCGGCACGCCAAACTGCGCTGAAATTAcc                                                                                                             >  2:359242/1‑140 (MQ=255)                        caccacCGGCTTACGCCCGAAGCTGTCTGCCATCGGCCCGTAGATTAACTGCCCCAACGCAAAGCCCAGAATATAAGTACTGAGGGTCATCTGCGTACTGCCCGCCGGCACGCCAAACTGCGCTGAAATTACCGGTAgcg                                                                                                      <  1:406537/140‑1 (MQ=255)                        caccacCGGCTTACGCCCGAAGCTGTCTGCCATCGGCCCGTAGATTAACTGCCCCAACGCAAAGCCCAGAATATAAGTACTGAGGGTCATCTGCGTACTGCCCGCCGGCACGCCAAACTGCGCTGAAATTACCGGTAgcg                                                                                                      <  1:955160/140‑1 (MQ=255)                        caccacCGGCTTACGCCCGAAGCTGTCTGCCATCGGCCCGTAGATTAACTGCCCCAACGCAAAGCCCAGAATATAAGTACTGAGGGTCATCTGCGTACTGCCCGCCGGCACGCCAAACTGCGCTGAAATTACCGGTAgcg                                                                                                      <  2:399485/140‑1 (MQ=255)                            accGGCTTACGCCCGAAGCTGTCTGCCATCGGCCCGTAGATTAACTGCCCCAACGCAAAGCCCAGAATATAAGTACTGAGGGTCATCTGCGTACTGCCCGCCGGCACGCCAAACTGCGCTGAAATTACCGGTAGCGCggg                                                                                                  <  2:184717/140‑1 (MQ=255)                             ccGGCTTACGCCCGAAGCTGTCTGCCATCGGCCCGTAGATTAACTGCCCCAACGCAAAGCCCAGAATATAAGTACTGAGGGTCATCTGCGTACTGCCCGCCGGCACGCCAAACTGCGCTGAAATTACCGGTAGCGCGGGc                                                                                                 >  2:520927/1‑140 (MQ=255)                             ccGGCTTACGCCCGAAGCTGTCTGCCATCGGCCCGTAGATTAACTGCCCCAACGCAAAGCCCAGAATATAAGTACTGAGGGTCATCTGCGTACTGCCCGCCGGCACGCCAAACTGCGCTGAAATTACCGGTAGCGCGGGc                                                                                                 <  2:642486/140‑1 (MQ=255)                                     cGCCCGAAGCTGTCTGCCATCGGCCCGTAGATTAACTGCCCCAACGCAAAGCCCAGAATATAAGTACTGAGGGTCATCTGCGTACTGCCCGCCGGCACGCCAAACTGCGCTGAAATTACCGGTAGCGCGGGCAGATACat                                                                                         >  1:80377/1‑140 (MQ=255)                                                gTCTGCCATCGGCCCGTAGATTAACTGCCCCAACGCAAAGCCCAGAATATAAGTACTGAGGGTCATCTGCGTACTGCCCGCCGGCACGCCAAACTGCGCTGAAATTACCGGTAGCGCGGGCAGATACATATCAATCGACa                                                                              <  1:359242/140‑1 (MQ=255)                                                        tCGGCCCGTAGATTAACTGCCCCAACGCAAAGCCCAGAATATAAGTACTGAGGGTCATCTGCGTACTGCCCGCCGGCACGCCAAACTGCGCTGAAATTACCGGTAGCGCGGGCAGATACATATCAATCGACAGCGGCATc                                                                      >  1:610975/1‑140 (MQ=255)                                                           gCCCGTAGATTAACTGCCCCAACGCAAAGCCCAGAATATAAGTACTGAGGGTCATCTGCGTACTGCCCGCCGGCACGCCAAACTGCGCTGAAATTACCGGTAGCGCGGGCAGATACATATCAATCGACAGCGGCATCAAc                                                                   >  1:513726/1‑140 (MQ=255)                                                               gTAGATTAACTGCCCCAACGCAAAGCCCAGAATATAAGTACTGAGGGTCATCTGCGTACTGCCCGCCGGCACGCCAAACTGCGCTGAAATTACCGGTAGCGCGGGCAGATACATATCAATCGACAGCGGCATCAACATgg                                                               <  1:520927/140‑1 (MQ=255)                                                                   aTTAACTGCCCCAACGCAAAGCCCAGAATATAAGTACTGAGGGTCATCTGCGTACTGCCCGCCGGCACGCCAAACTGCGCTGAAATTACCGGTAGCGCGGGCAGATACATATCAATCGACAGCGGCATCAACATGGCcag                                                           <  2:464322/140‑1 (MQ=255)                                                                        cTGCCCCAACGCAAAGCCCAGAATATAAGTACTGAGGGTCATCTGCGTACTGCCCGCCGGCACGCCAAACTGCGCTGAAATTACCGGTAGCGCGGGCAGATACATATCAATCGACAGCGGCATCAACATg                                                                <  2:863064/130‑1 (MQ=255)                                                                        cTGCCCCAACGCAAAGCCCAGAATATAAGTACTGAGGGTCATCTGCGTACTGCCCGCCGGCACGCCAAACTGCGCTGAAATTACCGGTAGCGCGGGCAGATACATATCAATCGACAGCGGCATCAACATg                                                                >  1:863064/1‑130 (MQ=255)                                                                          gCCCCAACGCAAAGCCCAGAATATAAGTACTGAGGGTCATCTGCGTACTGCCCGCCGGCACGCCAAACTGCGCTGAAATTACCGGTAGCGCGGGCAGATACATATCAATCGACAGCGGCATCAACATGGCCAGCAGGCCa                                                    <  1:825581/140‑1 (MQ=255)                                                                           ccccAACGCAAAGCCCAGAATATAAGTACTGAGGGTCATCTGCGTACTGCCCGCCGGCACGCCAAACTGCGCTGAAATTACCGGTAGCGCGGGCAGATACATATCAATCGACAGCGGCATCAACATGGCCAGCAGGCCaa                                                   >  2:487963/1‑140 (MQ=255)                                                                                        cccAGAATATAAGTACTGAGGGTCATCTGCGTACTGCCCGCCGGCACGCCAAACTGCGCTGAAATTACCGGTAGCGCGGGCAGATACATATCAATCGACAGCGGCATCAACATGGCCAGCAGGCCAAGGATAAAAACAAt                                      <  2:620152/140‑1 (MQ=255)                                                                                             aaTATAAGTACTGAGGGTCATCTGCGTACTGCCCGCCGGCACGCCAAACTGCGCTGAAATTACCGGTAGCGCGGGCAGATACATATCAATCGACAGCGGCATCAACATGGCCAGCAGGCCAAGGATAAAAACAATAGCaa                                 <  2:513726/140‑1 (MQ=255)                                                                                                       cTGAGGGTCATCTGCGTACTGCCCGCCGGCACGCCAAACTGCGCTGAAATTACCGGTAGCGCGGGCAGATACATATCAATCGACAGCGGCATCAACATGGCCAGCAGGCCAAGGATAAAAACAATAGCAAACGACGAATg                       >  2:675274/1‑140 (MQ=255)                                                                                                       cTGAGGGTCATCTGCGTACTGCCCGCCGGCACGCCAAACTGCGCTGAAATTACCGGTAGCGCGGGCAGATACATATCAATCGACAGCGGCATCAACATGGCCAGCAGGCCAAGGATAAAAACAATAGCAAACGACGAATg                       >  1:132843/1‑140 (MQ=255)                                                                                                         gAGGGTCATCTGCGTACTGCCCGCCGGCACGCCAAACTGCGCTGAAATTACCGGTAGCGCGGGCAGATACATATCAATCGACAGCGGCATCAACATGGCCAGCAGGCCAAGGATAAAAACAATAGCAAACGACGAATGCt                     >  2:631661/1‑140 (MQ=255)                                                                                                          aGGGTCATCTGCGTACTGCCCGCCGGCACGCCAAACTGCGCTGAAATTACCGGTAGCGCGGGCAGATACATATCAATCGACAGCGGCATCAACATGGCCAGCAGGCCAAGGATAAAAACAATAGCAAACGACGAATGCTg                    >  1:676429/1‑140 (MQ=255)                                                                                                          aGGGTCATCTGCGTACTGCCCGCCGGCACGCCAAACTGCGCTGAAATTACCGGTAGCGCGGGCAGATACATATCAATCGACAGCGGCATCAACATGGCCAGCAGGCCAAGGATAAAAACAATAGCAAACGACGAATGCTg                    >  1:219139/1‑140 (MQ=255)                                                                                                            ggTCATCTGCGTACTGCCCGCCGGCACGCCAAACTGCGCTGAAATTACCGGTAGCGCGGGCAGATACATATCAATCGACAGCGGCATCAACATGGCCAGCAGGCCAAGGATAAAAACAATAGCAAACGACGAAt                        <  2:939687/134‑1 (MQ=255)                                                                                                            ggTCATCTGCGTACTGCCCGCCGGCACGCCAAACTGCGCTGAAATTACCGGTAGCGCGGGCAGATACATATCAATCGACAGCGGCATCAACATGGCCAGCAGGCCAAGGATAAAAACAATAGCAAACGACGAAt                        >  1:939687/1‑134 (MQ=255)                                                                                                              tCATCTGCGTACTGCCCGCCGGCACGCCAAACTGCGCTGAAATTACCGGTAGCGCGGGCAGATACATATCAATCGACAGCGGCATCAACATGGCCAGCAGGCCAAGGATAAAAACAATAGCAAACGACGAATGCTGTCgg                <  2:735279/140‑1 (MQ=255)                                                                                                               cATCTGCGTACTGCCCGCCGGCACGCCAAACTGCGCTGAAATTACCGGTAGCGCGGGCAGATACATATCAATCGACAGCGGCATCAACATGGCCAGCAGGCCAAGGATAAAAACAATAGCGAACGACGAATGCTGTCggg               >  2:573320/1‑140 (MQ=255)                                                                                                               cATCTGCGTACTGCCCGCCGGCACGCCAAACTGCGCTGAAATTACCGGTAGCGCGGGCAGATACATATCAATCGACAGCGGCATCAACATGGCCAGCAGGCCAAGGATAAAAACAATAGCAAAcgacga                          >  1:717801/1‑129 (MQ=255)                                                                                                               cATCTGCGTACTGCCCGCCGGCACGCCAAACTGCGCTGAAATTACCGGTAGCGCGGGCAGATACATATCAATCGACAGCGGCATCAACATGGCCAGCAGGCCAAGGATAAAAACAATAGCAAAcgacga                          <  2:717801/129‑1 (MQ=255)                                                                                                                 tCTGCGTACTGCCCGCCGGCACGCCAAACTGCGCTGAAATTACCGGTAGCGCGGGCAGATACATATCAATCGACAGCGGCATCAACATGGCCAGCAGGCCAAGGATAAAAACAATAGCAAACGACGAATGCTGTCGggtg             >  2:48480/1‑140 (MQ=255)                                                                                                                  cTGCGTACTGCCCGCCGGCACGCCAAACTGCGCTGAAATTACCGGTAGCGCGGGCAGATACATATCAATCGACAGCGGCATCAACATGGCCAGCAGGCCAAGGATAAAAACAATAGCAAACGACGAATGCTGTCGggtgg            >  1:374196/1‑140 (MQ=255)                                                                                                                            cccGCCGGCACGCCAAACTGCGCTGAAATTACCGGTAGCGCGGGCAGATACATATCAATCGACAGCGGCATCAACATGGCCAGCAGGCCAAGGATAAAAACAATAGCAAACGACGAATGCTGTCGGGTGGTCACAACggg  <  2:132843/140‑1 (MQ=255)                                                                                                                            cccGCCGGCACGCCAAACTGCGCTGAAATTACCGGTAGCGCGGGCAGATACATATCAATCGACAGCGGCATCAACATGGCCAGCAGGCCAAGGATAAAAACAATAGCAAACGACGAATGCTGTCGGGTGGTCACAACggg  >  2:840233/1‑140 (MQ=255)                                                                                                                            cccGCCGGCACGCCAAACTGCGCTGAAATTACCGGTAGCGCGGGCAGATACATATCAATCGACAGCGGCATCAACATGGCCAGCAGGCCAAGGATAAAAACAATAGCAAACGACGAATGCTGTCGGGTGGTCACAACggg  <  1:675274/140‑1 (MQ=255)                                                                                                                            cccGCCGGCACGCCAAACTGCGCTGAAATTACCGGTAGCGCGGGCAGATACATATCAATCGACAGCGGCATCAACATGGCCAGCAGGCCAAGGATAAAAACAATAGCAAACGACGAATGCTGTCGGGTGGTAACAACggg  >  1:794583/1‑140 (MQ=255)                                                                                                                                    |                                                                                                                                    GCAAACACCAGCGTACCGCCGAGCACCACCGGCTTACGCCCGAAGCTGTCTGCCATCGGCCCGTAGATTAACTGCCCCAACGCAAAGCCCAGAATATAAGTACTGAGGGTCATCTGCGTACTGCCCGCCGGTACGCCAAACTGCGCTGAAATTACCGGTAGCGCGGGCAGATACATATCAATCGACAGCGGCATCAACATGGCCAGCAGGCCAAGGATAAAAACAATAGCAAACGACGAATGCTGTCGGGTGGTCACAACGGG  >  NC_000913/2279504‑2279766

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑