Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	352,853	C→T	R50* (CGA→TGA)	prpE →	propionyl‑CoA synthetase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	352,853	0	C	T	100.0%	108.6 / NA	32	R50* (CGA→TGA)	prpE	propionyl‑CoA synthetase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (22/10);  total (22/10)

ACGAACCGGAGCAGTTCTGGGCCGAGCAGGCCCGGCGTATTGACTGGCAGACGCCCTTTACGCAAACGCTCGATCACAGCAATCCGCCGTTTGCCCGTTGGTTTTGTGAAGGCCGAACCAACTTGTGCCACAACGCCATCGACCGCTGGCTGGAGAAACAGCCAGAGGCGCTGGCGCTGATTGCCGTCTCTTCGGAAACAGAAGAAGAGCGCACCTTTACCTTTCGTCAGCTGCATGACGA  >  NC_000913/352740‑352980                                                                                                                  |                                                                                                                                aCGAACCGGAGCAGTTCTGGGCCGAGCAGGCCCGGCGTATTGACTGGCAGACGCCCTTTACGCAAACGCTCGATCACAGCAATCCGCCGTTTGCCCGTTGGTTTTGTGAAGGCTGAACCAACTTGTGCCACAACGCCATc                                                                                                       >  1:955071/1‑140 (MQ=255)      ccGGAGCAGTTCTGGGCCGAGCAGGCCCGGCGTATTGACTGGCAGACGCCCTTTACGCAAACGCTCGATCACAGCAATCCGCCGTTTGCCCGTTGGTTTTGTGAAGGCTGAACCAACTTGTGCCACAACGCCATCGACCg                                                                                                  <  1:102920/140‑1 (MQ=255)      ccGGAGCAGTTCTGGGCCGAGCAGGCCCGGCGTATTGACTGGCAGACGCCCTTTACGCAAACGCTCGATCACAGCAATCCGCCGTTTGCCCGTTGGATTTGTGAAGGCTGAACCAACTTGTGCCACAACGCCATCGACCg                                                                                                  >  2:901058/1‑140 (MQ=255)                 cTGGGCCGAGCAGGCCCGGCGTATTGACTGGCAGACGCCCTTTACGCAAACGCTCGATCACAGCAATCCGCCGTTTGCCCGTTGGTTTTGTGAAGGCTGAACCAACTTGTGCCACAACGCCATCGACCGCTGGCTGgaga                                                                                       >  1:71235/1‑140 (MQ=255)                  tGGGCCGAGCAGGCCCGGCGTATTGACTGGCAGACGCCCTTTACGCAAACGCTCGATCACAGCAATCCGCCGTTTGCCCGTTGGTTTTGTGAAGGCTGAACCAACTTGTGCCACAACGCCATCGACCGCTGGCTGGAGaa                                                                                      <  2:336589/140‑1 (MQ=255)                         aGCAGGCCCGGCGTATTGACTGGCAGACGCCCTTTACGCAAACGCTCGATCACAGCAATCCGCCGTTTGCCCGTTGGTTTTGTGAAGGCTGAACCAACTTGTGCCACAACGCCATCGACCGCTGGCTGGAGAAACAGCCa                                                                               >  2:364403/1‑140 (MQ=255)                         aGCAGGCCCGGCGTATTGACTGGCAGACGCCCTTTACGCAAACGCTCGATCACAGCAATCCGCCGTTTGCCCGTTGGTTTTGTGAAGGCTGAACCAACTTGTGCCACAACGCCATCGACCGCTGGCTGGAGAAACAGCCa                                                                               >  1:905685/1‑140 (MQ=255)                                   gCGTATTGACTGGCAGACGCCCTTTACGCAAACGCTCGATCACAGCAATCCGCCGTTTGCCCGTTGGTTTTGTGAAGGCTGAACCAACTTGTGCCACAACGCCATCGACCGCTGGCTGGAGAAACAGCCAGAggcgctgg                                                                     >  2:472772/1‑140 (MQ=255)                                                cAGACGCCCTTTACGCAAACGCTCGATCACAGCAATCCGCCGTTTGCCCGTTGGTTTTGTGAAGGCTGAACCAACTTGTGCCACAACGCCATCGACCGCTGGCTGGAGAAACAGCCAGAGGCGCTGGCGCTGATTGCCGt                                                        <  2:71235/140‑1 (MQ=255)                                                   aCGCCCTTTACGCAAACGCTCGATCACAGCAATCCGCCGTTTGCCCGTTGGTTTTGTGAAGGCTGAACCAACTTGTGCCACAACGCCATCGACCGCTGGCTGGAGAAACAGCCAGAGGCGCTGGCGCTGATTGCCGtctc                                                     >  1:467623/1‑140 (MQ=255)                                                     gCCCTTTACGCAAACGCTCGATCACAGCAATCCGCCGTTTGCCCGTTGGTTTTGTGAAGGCTGAACCAACTTGTGCCACAACGCCATCGACCGCTGGCTGGAGAAACAGCCAGAGGCGCTGGCGCTGATTGCCGTCTCtt                                                   >  1:924932/1‑140 (MQ=255)                                                          ttACGCAAACGCTCGATCACAGCAATCCGCCGTTTGCCCGTTGGTTTTGTGAAGGCTGAACCAACTTGTGCCACAACGCCATCGACCGCTGGCTGGAGAAACAGCCAGAGGCGCTGGCGCTGATTGCCGTCTCTTCGGaa                                              >  1:33162/1‑140 (MQ=255)                                                           tACGCAAACGCTCGATCACAGCAATCCGCCGTTTGCCCGTTGGTTTTGTGAAGGCTGAACCAACTTGTGCCACAACGCCATCGACCGCTGGCTGGAGAAACAGCCAGAGGCGCTGGCGCTGATTGCCGTCTCTTCGGaaa                                             >  1:111270/1‑140 (MQ=255)                                                                 aaCGCTCGATCACAGCAATCCGCCGTTTGCCCGTTGGTTTTGTGAAGGCTGAACCAACTTGTGCCACAACGCCATCGACCGCTGGCTGGAGAAACAGCCAGAGGCGCTGGCGCTGATTGCCGTCTCTTCGGAAACagaag                                       >  2:376833/1‑140 (MQ=255)                                                                 aaCGCTCGATCACAGCAATCCGCCGTTTGCCCGTTGGTTTTGTGAAGGCTGAACCAACTTGTGCCACAACGCCATCGACCGCTGGCTGGAGAAACAGCCAGAGGCGCTGGCGCTGATTGCCGTCTCTTCGGAAACagaag                                       >  1:228002/1‑140 (MQ=255)                                                                  aCGCTCGATCACAGCAATCCGCCGTTTGCCCGTTGGTTTTGTGAAGGCTGAACCAACTTGTGCCACAACGCCATCGACCGCTGGCTGGAGAAACAGCCAGAGGCGCTGGCGCTGATTGCCGTCTCTTCGGAAACagaaga                                      >  2:408970/1‑140 (MQ=255)                                                                   cGCTCGATCACAGCAATCCGCCGTTTGCCCGTTGGTTTTGTGAAGGCTGAACCAACTTGTGCCACAACGCCATCGACCGCTGGCTGGAGAAACAGCCAGAGGCGCTGGCGCTGATTGCCGTCTCTTCGGAAACagaagaa                                     >  2:307525/1‑140 (MQ=255)                                                                   cGCTCGATCACAGCAATCCGCCGTTTGCCCGTTGGTTT‑‑TGAAGGCTGAACCAACTTGTGCCACAACGCCATCGACCGCTGGCTGGAGAAACAGCCAGAGGCGCTGGCGCTGATTGCCGTCTCTTCGGAAACagaagaaga                                   >  2:539897/1‑140 (MQ=255)                                                                       cGATCACAGCAATCCGCCGTTTGCCCGTTGGTTTTGTGAAGGCTGAACCAACTTGTGCCACAACGCCATCGACCGCTGGCTGGAGAAACAGCCAGAGGCGCTGGCGCTGATTGCCGTCTCTTCGGAAACAGAAGAAGAgc                                 <  2:639649/140‑1 (MQ=255)                                                                        gATCACAGCAATCCGCCGTTTGCCCGTTGGTTTTGTGAAGGCTGAACCAACTTGTGCCACAACGCCATCGACCGCTGGCTGGAGAAACAGCCAGAGGCGCTGGCGCTGATTGCCGTCTCTTCGGAAACAGAAGAAGAgcg                                <  1:307525/140‑1 (MQ=255)                                                                              aGCAATCCGCCGTTTGCCCGTTGGTTTTGTGAAGGCTGAACCAACTTGTGCCACAACGCCATCGACCGCTGGCTGGAGAAACAGCCAGAGGCGCTGGCGCTGATTGCCGTCTCTTCGGAAACAGAAGAAGAGCGCacctt                          >  2:966632/1‑140 (MQ=255)                                                                                cAATCCGCCGTTTGCCCGTTGGTTTTGTGAAGGCTGAACCAACTTGTGCCACAACGCCATCGACCGCTGGCTGGAGAAACAGCCAGAGGCGCTGGCGCTGATTGCCGTCTCTTCGGAAACAGAAGAAGAGCGCaccttta                        <  2:924932/140‑1 (MQ=255)                                                                                 actCCGCCGTTTGCCCGTTGGTTTTGTGAAGGCTGAACCAACTTGTGCCACAACGCCATCGACCGCTGGCTGGAGAAACAGCCAGAGGCGCTGGCGCTGATTGCCGTCTCTTCGGAAACAGAAGAAGAGCGCacctttac                       <  2:898518/138‑1 (MQ=255)                                                                                 aaTCCGCCGTTTGCCCGTTGGTTTTGTGAAGGCTGAACCAACTTGTGCCACAACGCCATCGACCGCTGGCTGGAGAAACAGCCAGAGGCGCTGGCGCTGATTGCCGTCTCTTCGGAAACAGAAGAAGAGCGCacctttac                       <  1:408970/140‑1 (MQ=255)                                                                                   tCCGCCGTTTGCCCGTTGGTTTTGTGAAGGCTGAACCAACTTGTGCCACAACGCCATCGACCGCTGGCTGGAGAAACAGCCAGAGGCGCTGGCGCTGATTGCCGTCTCTTCGGAAACAGAAGAAGAGCGCacctttacct                     >  1:763880/1‑140 (MQ=255)                                                                                   tCCGCCGTTTGCCCGTTGGTTTTGTGAAGGCTGAACCAACTTGTGCCACAACGCCATCGACCGCTGGCTGGAGAAACAGCCAGAGGCGCTGGCGCTGATTGCCGTCTCTTCGGAAACAGAAGAAGAGCGCacctttacct                     >  1:491708/1‑140 (MQ=255)                                                                                    ccgccgTTTGCCCGTTGGTTTTGTGAAGGCTGAACCAACTTGTGCCACAACGCCATCGACCGCTGGCTGGAGAAACAGCCAGAGGCGCTGGCGCTGATTGCCGTCTCTTCGGAAACAGAAGAAGAGCGCacctttacctt                    >  2:432915/1‑140 (MQ=255)                                                                                     cgccgTTTGCCCGTTGGTTTTGTGAAGGCTGAACCAACTTGTGCCACAACGCCATCGACCGCTGGCTGGAGAAACAGCCAGAGGCGCTGGCGCTGATTGCCGTCTCTTCGGAAACAGAAGAAGAGCGCacctttaccttt                   >  2:190074/1‑140 (MQ=255)                                                                                         gTTTGCCCGTTGGTTTTGTGAAGGCTGAACCAACTTGTGCCAAAACGCCATCGACCGCTGGCTGGAGAAACAGCCAGAGGCGCTGGCGCTGATTGCCGTCTCTTCGGAAACAGAAGAAGAGCGCACCTTTACCTTTCGTc               <  2:905685/140‑1 (MQ=255)                                                                                          tttGCCCGTTGGTTTTGTGAAGGCTGAACCAACTTGTGCCACAACGCCATCGACCGCTGGCTGGAGAAACAGCCAGAGGCGCTGGCGCTGATTGCCGTCTCTTCGGAAACAGAAGAAGAGCGCa                              >  2:362665/1‑124 (MQ=255)                                                                                          tttGCCCGTTGGTTTTGTGAAGGCTGAACCAACTTGTGCCACAACGCCATCGACCGCTGGCTGGAGAAACAGCCAGAGGCGCTGGCGCTGATTGCCGTCTCTTCGGAAACAGAAGAAGAGCGCa                              <  1:362665/124‑1 (MQ=255)                                                                                                      ttttGTGAAGGCTGAACCAACTTGTGCCACAACGCCATCGACCGCTGGCTGGAGAAACAGCCAGAGGCGCTGGCGCTGATTGCCGTCTCTTCGGAAACAGAAGAAGAGCGCACCTTTACCTTTCGTCAGCTGCATGACGa  >  1:766136/1‑140 (MQ=255)                                                                                                                  |                                                                                                                                ACGAACCGGAGCAGTTCTGGGCCGAGCAGGCCCGGCGTATTGACTGGCAGACGCCCTTTACGCAAACGCTCGATCACAGCAATCCGCCGTTTGCCCGTTGGTTTTGTGAAGGCCGAACCAACTTGTGCCACAACGCCATCGACCGCTGGCTGGAGAAACAGCCAGAGGCGCTGGCGCTGATTGCCGTCTCTTCGGAAACAGAAGAAGAGCGCACCTTTACCTTTCGTCAGCTGCATGACGA  >  NC_000913/352740‑352980

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑