Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,327,646	T→C	E166E (GAA→GAG)	yfaP ←	DUF2135 domain‑containing protein YfaP

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,327,646	0	T	C	100.0%	225.9 / NA	62	E166E (GAA→GAG)	yfaP	DUF2135 domain‑containing protein YfaP
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (33/29);  total (33/29)

AGTAGTTGATATACACCTGATAGCGCCCGTGAATTGGCGCGGGCATGGCGAAAATCTCGGGTCCGTACCCCGTCGTGACATCCATATCCAGTGCACCACTGTTTTTCAGCACGGTGTTACCGTACCAGGCGTGTTCGCCATCGGGCGTAACAACGTGAAGGTCGAGGTCGGTATTGTCCGTATCCCACGAGAGAACCAGCCGTAAACGTGCACGAATCGTTCCTGTACCCGGCGTTGAGTAAAACTGCATTTTTTGTCGGCTTTGC  >  NC_000913/2327513‑2327778                                                                                                                                      |                                                                                                                                     agtagtTGATATACACCTGATAGCGCCCGTGAATTGGCGCGGGCATGGCGAAAATCTCGGGTCCGTACCCCGTCGTGACATCCATATCCAGTGCACCACTGTTTTTCAGCACGGTGTTACCGTACCAGGCGTGCTCGCCa                                                                                                                                >  2:937345/1‑140 (MQ=255) agtagtTGATATACACCTGATAGCGCCCGTGAATTGGCGCGGGCATGGCGAAAATCTCGGGTCCGTACCCCGTCGTGACATCCATATCCAGTGCACCACTGTTTTTCAGCACGGTGTTACCGTACCAGGCGTGCTCGCCa                                                                                                                                <  1:728941/140‑1 (MQ=255)   tagtTGATATACACCTGATAGCGCCCGTGAATTGGCGCGGGCATGGCGAAAATCTCGGGTCCGTACCCCGTCGTGACATCCATATCCAGTGCACCACTGTTTTTCAGCACGGTGTTACCGTACCAGGCGTGCTCGCCATc                                                                                                                              >  2:527283/1‑140 (MQ=255)   tagtTGATATACACCTGATAGCGCCCGTGAATTGGCGCGGGCATGGCGAAAATCTCGGGTCCGTACCCCGTCGTGACATCCATATCCAGTGCACCACTGTTTTTCAGCACGGTGTTACCGTACCAGGCGTGCTCGCCATc                                                                                                                              <  1:359277/140‑1 (MQ=255)        gATATACACCTGATAGCGCCCGTGAATTGGCGCGGGCATGGCGAAAATCTCGGGTCCGTACCCCGTCGTGACATCCATATCCAGTGCACCACTGTTTTTCAGCACGGTGTTACCGTACCAGGCGTGCTCGCCATCGGGCg                                                                                                                         >  1:762157/1‑140 (MQ=255)           ataCACCTGATAGCGCCCGTGAATTGGCGCGGGCATGGCGAAAATCTCGGGTCCGTACCCCGTCGTGACATCCATATCCAGTGCACCACTGTTTTTCAGCACGGTGTTACCGTACCAGGCGTGCTCGCCATCGGGCGTaa                                                                                                                      <  1:446179/140‑1 (MQ=255)             acacCTGATAGCGCCCGTGAATTGGCGCGGGCATGGCGAAAATCTCGGGTCCGTACCCCGTCGTGACATCCATATCCAGTGCACCACTGTTTTTCAGCACGGTGTTACCGTACCAGGCGTGCTCGCCATCGGGCGTaaca                                                                                                                    >  2:5336/1‑140 (MQ=255)             acacCTGATAGCGCCCGTGAATTGGCGCGGGCATGGCGAAAATCTCGGGTCCGTACCCCGTCGTGACAT‑CATATCCAGTGCACCACTGTTTTTCAGCACGGTGTTACCGTACCAGGCGTGCTCGCCATCGGGCGTaacaa                                                                                                                   >  2:2838/1‑140 (MQ=255)                ccTGATAGCGCCCGTGAATTGGCGCGGGCATGGCGAAAATCTAGGGTCCGTACCCCGTCGTGACATCCATATCCAGTGCACCACTGTTTTTCAGCACGGTGTTACCGTACCAGGCGTGCTCGCCATCGGGCGTAACAACg                                                                                                                 >  2:543234/1‑140 (MQ=255)                   gATAGCGCCCGTGAATTGGCGCGGGCATGGCGAAAATCTCGGGTCCGTACCCCGTCGTGACATCCATATCCAGTGCACCACTGTTTTTCAGCACGGTGTTACCGTACCAGGCGTGCTCGCCATCGGGCGTAACAACGTGa                                                                                                              >  1:219225/1‑140 (MQ=255)                   gATAGCGCCCGTGAATTGGCGCGGGCATGGCGAAAATCTCGGGTCCGTACCCCGTCGTGACATCCATATCCAGTGCACCACTGTTTTTCAGCACGGTGTTACCGTACCAGGCGTGCTCGCCATCGGGCGTAACAACGTGa                                                                                                              >  1:438662/1‑140 (MQ=255)                    aTAGCGCCCGTGAATTGGCGCGGGCATGGCGAAAATCTCGGGTCCGTACCCCGTCGTGACATCCATATCCAGTGCACCACTGTTTTTCAGCACGGTGTTACCGTACCAGGCGTGCTCGCCATCGGGCGTAACAACGTGaa                                                                                                             >  2:321935/1‑140 (MQ=255)                    aTAGCGCCCGTGAATTGGCGCGGGCATGGCGAAAATCTCGGGTCCGTACCCCGTCGTGACATCCATATCCAGTGCACCACTGTTTTTCAGCACGGTGTTACCGTACCAGGCGTGCTCGCCATCGGGCGTAACAACGTGaa                                                                                                             >  2:867377/1‑140 (MQ=255)                    aTAGCGCCCGTGAATTGGCGCGGGCATGGCGAAAATCTCGGGTCCGTACCCCGTCGTGACATCCATATCCAGTGCACCACTGTTTTTCAGCACGGTGTTACCGTACCAGGCGTGCTCGCCATCGGGCGTAACAACGTGaa                                                                                                             >  1:785959/1‑140 (MQ=255)                      aGCGCCCGTGAATTGGCGCGGGCATGGCGAAAATCTAGGGTCCGTACCCCGTCGTGACATCCATATCCAGTGCACCACTGTTTTTCAGCACGGTGTTACCGTACCAGGCGTGCTCGCCATCGGGCGTAACAACGTGAagg                                                                                                           <  1:543234/140‑1 (MQ=255)                         gcCCGTGAATTGGCGCGGGCATGGCGAAAATCTCGGGTCCGTACCCCGTCGTGACATCCATATCCAGTGCACCACTGTTTTTCAGCACGGTGTTACCGTACCAGGCGTGCTCGCCATCGGGCGTAACAACGTGAaggtcg                                                                                                        >  2:107432/1‑140 (MQ=255)                         gcCCGTGAATTGGCGCGGGCATGGCGAAAATCTCGGGTCCGTACCCCGTCGTGACAT‑CATATCCAGTGCACCACTGTTTTTCAGCACGGTGTTACCGTACCAGGCGTGCTCGCCATCGGGCGTAACAACGTGAaggtcga                                                                                                       <  1:2838/140‑1 (MQ=255)                         gcCCGTGAATTGGCGC‑GGCATGGCGAAAATCTCGGGTCCGTACCCCGTCGTGACATCCATATCCAGTGCACCACTGTTTTTCAGCACGGTGTTACCGTACCAGGCGTGCTCGCCATCGGGCGTAACAACGTGAaggtcga                                                                                                       >  1:963712/1‑140 (MQ=255)                          cccGTGAATTGGCGCGGGCATGGCGAAAATCTCGGGTCCGTACCCCGTCGTGACATCCATATCCAGTGCACCACTGTTTTTCAGCACGGTGTTACCGTACCAGGCGTGCTCGCCATCGGGCGTAACAACGTGAaggtcga                                                                                                       <  2:679674/140‑1 (MQ=255)                           ccGTGAATTGGCGCGGGCATGGCGAAAATCTCGGGTCCGTACCCCGTCGTGACATCCATATCCAGTGCACCACTGTTTTTCAGCACGGTGTTACCGTACCAGGCGTGCTCGCCATCGGGCGTAACAACGTGAaggtcgag                                                                                                      >  2:217450/1‑140 (MQ=255)                           ccGTGAATTGGCGCGGGCATGGCGAAAATCTCGGGTCCGTACCCCGTCGTGACATCCATATCCAGTGCACCACTGTTTTTCAGCACGGTGTTACCGTACCAGGCGTGCTCGCCATCGGGCGTAACAACGTGAaggtcgag                                                                                                      >  2:715060/1‑140 (MQ=255)                            cGTGAATTGGCGCGGGCATGGCGAAAATCTCGGGTCCGTACCCCGTCGTGACATCCATATCCAGTGCACCACTGTTTTTCAGCACGGTGTTACCGTACCAGGCGTGCTCGCCATCGGGCGTAACAACGTGAaggtcgagg                                                                                                     <  2:762157/140‑1 (MQ=255)                                 aTTGGCGCGGGCATGGCGAAAATCTCGGGTCCGTACCCCGTCGTGAAATCCATATCCAGTGCACCACTGTTTTTCAGCACGGTGTTACCGTACCAGGCGTGCTCGCCATCGGGCGTAACAACGTGAAGGTCGAGGTCGGt                                                                                                <  1:232578/140‑1 (MQ=255)                                  ttGGCGCGGGCATGGCGAAAATCTCGGGTCCGTACCCCGTCGTGACATCCATATCCAGTGCACCACTGTTTTTCAGCACGGTGTTACCGTACCAGGCGTGCTCGCCATCGGGCGTAACAACGTGAAGGTCGAGGTCGGTa                                                                                               <  1:217450/140‑1 (MQ=255)                                  ttGGCGCGGGCATGGCGAAAATCTCGGGTCCGTACCCCGTCGTGACATCCATATCCAGTGCACCACTGTTTTTCAGCACGGTGTTACCGTACCAGGCGTGCTCGCCATCGGGCGTAACAACGTGAAGGTCGAGGTCGGTa                                                                                               <  1:5336/140‑1 (MQ=255)                                   tGGCGCGGGCATGGCGAAAATCTCGGGTCCGTACCCCGTCGTGACATCCATATCCAGTGCACCACTGTTTTTCAGCACGGTGTTACCGTACCAGGCGTGCTCGCCATCGGGCGTAACAACGTGAAGGTCGAGGTCGGTAt                                                                                              <  2:222196/140‑1 (MQ=255)                                       gcgGGCATGGCGAAAATCTCGGGTCCGTACCCCGTCGTGACATCCATATCCAGTGCACCACTGTTTTTCAGCACGGTGTTACCGTACCAGGCGTGCTCGCCATCGGGCGTAACAACGTGAAGGTCGAGGTCGGTATTGTc                                                                                          <  1:527283/140‑1 (MQ=255)                                         gggCATGGCGAAAATCTCGGGTCCGTACCCCGTCGTGACATCCATATCCAGTGCACCACTGTTTTTCAGCACGGTGTTACCGTACCAGGCGTGCTCGCCATCGGGCGTAACAACGTGAAGGTCGAGGTCGGTATTGTCCg                                                                                        <  1:121701/140‑1 (MQ=255)                                         gggCATGGCGAAAATCTCGGGTCCGTACCCCGTCGTGACATCCATATCCAGTGCACCACTGTTTTTCAGCACGGTGTTACCGTACCAGGCGTGCTCGCCATCGGGCGTAACAACGTGAAGGTCGAGGTCGGTATTGTCCg                                                                                        <  1:937345/140‑1 (MQ=255)                                               ggCGAAAATCTCGGGTCCGTACCCCGTCGTGACATCCATATCCAGTGCACCACTGTTTTTCAGCACGGTGTTACCGTACCAGGCGTGCTCGCCATCGGGCGTAACAACGTGAAGGTCGAGGTCGGt                                                                                                >  2:109793/1‑126 (MQ=255)                                               ggCGAAAATCTCGGGTCCGTACCCCGTCGTGACATCCATATCCAGTGCACCACTGTTTTTCAGCACGGTGTTACCGTACCAGGCGTGCTCGCCATCGGGCGTAACAACGTGAAGGTCGAGGTCGGt                                                                                                <  1:109793/126‑1 (MQ=255)                                               ggCGAAAATCTCGGGTCCGTACCCCGTCGTGACATCCATATCCAGTGCACCACTGTTTTTCAGCACGGTGTTACCGTACCAGGCGTGCTCGCCATCGGGCGTAACAACGTGAAGGTCGAGGTCGGTATTGTCCGTATccc                                                                                  <  1:107432/140‑1 (MQ=255)                                                     aaTCTCGGGTCCGTACCCCGTCGTGACATCCATATCCAGTGCACCACTGTTTTTCAGCACGGTGTTACCGTACCAGGCGTGCTCGCCATCGGGCGTAACAACGTGAAGGTCGAGGTCGGTATTGTCCGTATCCCACgaga                                                                            >  2:912983/1‑140 (MQ=255)                                                        ctcGGGTCCGTACCCCGTCGTGACATCCATATCCAGTGCACCACTGTTTTTCAGCACGGTGTTACCGTACCAGGCGTGCTCGCCATCGGGCGTAACAACGTGAAGGTCGAGGTCGGTATTGTCCGTATCCCACGAGAGaa                                                                         <  2:438662/140‑1 (MQ=255)                                                                         tcgtGACATCCATATCCAGTGCACCACTGTTTTTCAGCACGGTGTTACCGTACCAGGCGTGCTCGCCATCGGGCGTAACAACGTGAAGGTCGAGGTCGGTATTGTCCGTATCCCACGAGAGAACCAGCCGTAAACGTGCa                                                        <  1:543359/140‑1 (MQ=255)                                                                         tcgtGACATCCATATCCAGTGCACCACTGTTTTTCAGCACGGTGTTACCGTACCAGGCGTGCTCGCCATCGGGCGTAACAACGTGAAGGTCGAGGTCGGTATTGTCCGTATCCCACGAGAGAACCAGCCGTAAACGTGCa                                                        >  2:543359/1‑140 (MQ=255)                                                                          cgtGACATCCATATCCAGTGCACCACTGTTTTTCAGCACGGTGTTACCGTACCAGGCGTGCTCGCCATCGGGCGTAACAACGTGAAGGTCGAGGTCGGTATTGTCCGTATCCCACGAGAGAACCAGCCGTAAACGTGCAc                                                       >  1:908898/1‑140 (MQ=255)                                                                            tGACATCCATATCCAGTGCACCACTGTTTTTCAGCACGGTGTTACCGTACCAGGCGTGCTCGCCATCGGGCGTAACAACGTGAAGGTCGAGGTCGGTATTGTCCGTATCCCACGAGAGAACCAGCCGTAAACGTGCACGa                                                     >  1:559186/1‑140 (MQ=255)                                                                              aCATCCATATCCAGTGCACCACTGTTTTTCAGCACGGTGTTACCGTACCAGGCGTGCTCGCCATCGGGCGTAACAACGTGAAGGTCGAGGTCGGTATTGTCCGTATCCCACGAGAGAACCAGCCGTAAACGTGCACGAAt                                                   <  1:794104/140‑1 (MQ=255)                                                                              aCATCCATATCCAGTGCACCACTGTTTTTCAGCACGGTGTTACCGTACCAGGCGTGCTCGCCATCGGGCGTAACAACGTGAAGGTCGAGGTCGGTATTGTCCGTATCCCACGAGAGAACCAGCCGTAAACGTGCACGAAt                                                   <  2:785959/140‑1 (MQ=255)                                                                                aTCCATATCCAGTGCACCACTGTTTTTCAGCACGGTGTTACCGTACCAGGCGTGCTCGCCATCGGGCGTAACAACGTGAAGGTCGAGGTCGGTATTGTCCGTATCCCACGAGAGAACCAGCCGTAAACGTGCACGAATCg                                                 <  1:867377/140‑1 (MQ=255)                                                                                aTCCATATCCAGTGCACCACTGTTTTTCAGCACGGTGTTACCGTACCAGGCGTGCTCGCCATCGGGCGTAACAACGTGAAGGTCGAGGTCGGTATTGTCCGTATCCCACGAGAGAACCAGCCGTAAACGTGCACGAATCg                                                 <  1:321935/140‑1 (MQ=255)                                                                                   cATATCCAGTGCACCACTGTTTTTCAGCACGGTGTTACCGTACCAGGCGTGCTCGCCATCGGGCGTAACAACGTGAAGGTCGAGGTCGGTATTGTCCGTATCCCACGAGAGAACCAGCCGTAAACGTGCACGAATCGTTc                                              <  2:219225/140‑1 (MQ=255)                                                                                   cATATCCAGTGCACCACTGTTTTTCAGCACGGTGTTACCGTACCAGGCGTGCTCGCCATCGGGCGTAACAACGTGAAGGTCGAGGTCGGTATTGTCCGTATCCCACGAGAGAACCAGCCGTAAACGTGCACGAATCGTTc                                              >  1:582897/1‑140 (MQ=255)                                                                                           gTGCACCACTGTTTTTCAGCACGGTGTTACCGTACCAGGCGTGCTCGCCATCGGGCGTAACAACGTGAAGGTCGAGGTCGGTATTGTCCGTATCCCACGAGAGAACCAGCCGTAAACGTGCACGAATCGTTCCTGTAccc                                      >  1:913296/1‑140 (MQ=255)                                                                                            tGCACCACTGTTTTTCAGCACGGTGTTACCGTACCAGGCGTGCTCGCCATCGGGCGTAACAACGTGAAGGTCGAGGTCGGTATTGTCCGTATCCCACGAGAGAACCAGCCGTAAACGTGCACGAAt                                                   <  1:2729/126‑1 (MQ=255)                                                                                            tGCACCACTGTTTTTCAGCACGGTGTTACCGTACCAGGCGTGCTCGCCATCGGGCGTAACAACGTGAAGGTCGAGGTCGGTATTGTCCGTATCCCACGAGAGAACCAGCCGTAAACGTGCACGAAt                                                   >  2:2729/1‑126 (MQ=255)                                                                                            tGCACCACTGTTTTTCAGCACGGTGTTACCGTACCAGGCGTGCTCGCCATCGGGCGTAACAACGTGAAGGTCGAGGTCGGTATTGTCCGTATCCCACGAGAGAACCAGCCGTAAACGTGCACGAATCGTTCCTGTACCCg                                     >  1:944368/1‑140 (MQ=255)                                                                                            tGCACCACTGTTTTTCAGCACGGTGTTACCGTACCAGGCGTGCTCGCCATCGGGCGTAACAACGTGAAGGTCGAGGTCGGTATTGTCCGTATCCCACGAGAGAACCAGCCGTAAACGTGCACGAATCGTTCCTGTACCCg                                     >  2:474362/1‑140 (MQ=255)                                                                                            tGCACCACTGTTTTTCAGCACGGTGTTACCGTACCAGGCGTGCTCGCCATCGGGCGTAACAACGTGAAGGTCGAGGTCGGTATTGTCCGTATCCCACGAGAGAACCAGCCGTAAACGTGCACGAATCGTTCCTGTACCCg                                     >  2:308967/1‑140 (MQ=255)                                                                                            tGCACCACTGTTTTTCAGCACGGTGTTACCGTACCAGGCGTGCTCGCCATCGGGCGTAACAACGTGAAGGTCGAGGTCGGTATTGTCCGTATCCCACGAGAGAACCAGCCGTAAACGTGCACGAATCGTTCCTGTACCCg                                     >  2:287593/1‑140 (MQ=255)                                                                                            tGCACCACTGTTTTTCAGCACGGTGTTACCGTACCAGGCGTGCTCGCCATCGGGCGTAACAACGTGAAGGTCGAGGTCGGTATTGTCCGTATCCCACGAGAGAACCAGCCGTAAACGTGCACGAATCGTTCCTGTACCCg                                     >  1:464229/1‑140 (MQ=255)                                                                                               accacTGTTTTTCAGCACGGTGTTACCGTACCAGGCGTGCTCGCCATCGGGCGTAACAACGTGAAGGTCGAGGTCGGTATTGTCCGTATCCCACGAGAGAACCAGCCGTAAACGTGCACGAATCGTTCCTGTACCCGGCg                                  <  2:908898/140‑1 (MQ=255)                                                                                               accacTGTTTTTCAGCACGGTGTTACCGTACCAGGCGTGCTCGCCATCGGGCGTAACAACGTGAAGGTCGAGGTCGGTATTGTCCGTATCCCACGAGAGAACCAGCCGTAAACGTGCACGAATCGTTCCTGTACCCGGCg                                  <  2:963712/140‑1 (MQ=255)                                                                                                              cACGGTGTTACCGTACCAGGCGTGCTCGCCATCGGGCGTAACAACGTGAAGGTCGAGGTCGGTATTGTCCGTATCCCACGAGAGAACCAGCCGTAAACGTGCACGAATCGTTCCTGTACCCGGCGTTGAGTAAAACTGCa                   >  2:749488/1‑140 (MQ=255)                                                                                                                     ttACCGTACCAGGCGTGCTCGCCATCGGGCGTAACAACGTGAAGGTCGAGGTCGGTATTGTCCGTATCCCACGAGAGAACCAGCCGTAAACGTGCACGAATCGTTCCTGTACCCGGCGTTGAGTAAAACTGCATTTTTTg            <  1:474362/140‑1 (MQ=255)                                                                                                                      tACCGTACCAGGCGTGCTCGCCATCGGGCGTAACAACGTGAAGGTCGAGGTCGGTATTGTCCGTATCCCACGAGAGAACCAGCCGTAAACGTGCACGAATCGTTCCTGTACCCGGCGTTGAGTAAAACTGCATTTTTTGt           <  2:913296/140‑1 (MQ=255)                                                                                                                             ccAGGCGTGCTCGCCATCGGGCGTAACAACGTGAAGGTCGAGGTCGGTATTGTCCGTATCCCACGAGAGAACCAGCCGTAAACGTGCACGAATCGTTCCTGTACCCGGCGTTGAGTAAAACTGCATTTTTTGTCGGCttt    >  1:213651/1‑140 (MQ=255)                                                                                                                             ccAGGCGTGCTCGCCATCGGGCGTAACAACGTGAAGGTCGAGGTCGGTATTGTCCGTATCCCACGAGAGAACCAGCCGTAAACGTGCACGAATCGTTCCTGTACCCGGCGTTGAGTAAAACTGCATTTTTTGTCGGCttt    >  1:116207/1‑140 (MQ=255)                                                                                                                               aGGCGTGCTCGCCATCGGGCGTAACAACGTGAAGGTCGAGGTCGGTATTGTCCGTATCCCACGAGAGAACCAGCCGTAAACGTGCACGAATCGTTCCTGTACCCGGCGTTGAGTAAAACTGCATTTTTTGTCGGCTTTGc  >  2:196101/1‑140 (MQ=255)                                                                                                                                  cGTGCTCGCCATCGGGCGTAACAACGTGAAGGTCGAGGTCGGTATTGTCCGTATCCCACGAGAGAACCAGCCGTAAACGTGCACGAATCGTTCCTGTACCCGGCGTTGAGTAAAACTGCATTTTTTGTCgg        <  2:589003/131‑1 (MQ=255)                                                                                                                                  cGTGCTCGCCATCGGGCGTAACAACGTGAAGGTCGAGGTCGGTATTGTCCGTATCCCACGAGAGAACCAGCCGTAAACGTGCACGAATCGTTCCTGTACCCGGCGTTGAGTAAAACTGCATTTTTTGTCgg        >  1:589003/1‑131 (MQ=255)                                                                                                                                      |                                                                                                                                     AGTAGTTGATATACACCTGATAGCGCCCGTGAATTGGCGCGGGCATGGCGAAAATCTCGGGTCCGTACCCCGTCGTGACATCCATATCCAGTGCACCACTGTTTTTCAGCACGGTGTTACCGTACCAGGCGTGTTCGCCATCGGGCGTAACAACGTGAAGGTCGAGGTCGGTATTGTCCGTATCCCACGAGAGAACCAGCCGTAAACGTGCACGAATCGTTCCTGTACCCGGCGTTGAGTAAAACTGCATTTTTTGTCGGCTTTGC  >  NC_000913/2327513‑2327778

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑